The effects of boar seminal plasma extracellular vesicles on sperm fertility.

Extracellular vesicles (EVs) are abundant in body fluid and are critical in cell interaction. Seminal plasma contains numerous EVs which affecting sperm function via transferring regulatory cargoes to the sperm. However, the mechanism of seminal plasma extracellular vesicles (SP-EVs) is still not clear. The present study aimed to isolate the boar SP-EVs and explore its potential function, then identify the key protein involved in SP-EVs and sperms interaction, and elucidate mechanism of SP-EVs protein on sperms. Here, we successfully isolated and concentrated boar SP-EVs, the SP-EVs showed a typical vesicle structure under transmission electron microscopy, most of their diameters range between 50 and 200 nm and express EVs biomarkers CD9 and CD63. We proved that SP-EVs could inhibit sperm acrosome reaction and in vitro fertility. Through a data-independent acquisition analysis of protein profiles of noncapacitated sperms, normal capacitated sperms and SP-EVs treated capacitated sperms, we identified that EZRIN was one of the active proteins that participated in SP-EVs and sperms interaction. Furthermore, we tested that the inhibition of EZRIN could promote boar sperm fertility, which is in consistence with the function of SP-EVs. The results may facilitate future research of SP-EVs on sperm function and male infertility.

Association of exposure to ozone and fine particulate matter with ovarian reserve among women with infertility.

Evidence linking diminished ovarian reserve, a significant cause of female infertility, and exposure to particulate matter with aerodynamic diameters ≤2.5 µm (PM2.5) or O3 exposure remains a critical knowledge gap in female fertility. This study investigated the association between ambient PM2.5, O3 pollution, and anti-Müllerian hormone (AMH), a sensitive marker of ovarian reserve, in reproductive-aged Chinese women. We enrolled 2212 women with spontaneous menstrual cycles who underwent AMH measurements at a reproductive medicine center between 2018 and 2021. The daily mean concentrations of outdoor PM2.5 and O3 were estimated using a validated spatiotemporal model, followed by matching the participants' residential addresses. Three exposure periods were designed according to AMH expression patterns during follicle development. A generalized linear model was used to investigate changes in AMH associated with air pollution. The results showed a mean AMH level of 3.47 ± 2.61 ng/mL. During the six months from primary to early antral follicle stage (Period 1), each 10 µg/m3 increase in PM2.5 and O3 exposure was associated with AMH changes of -0.21 (95% confidence interval [CI]: -0.48, 0.06) ng/mL and -0.31 (95% CI: -0.50, -0.12) ng/mL, respectively. Further analyses indicated that the reduced ovarian reserve measured by AMH level was only significantly associated with PM2.5 exposure during follicle development from the primary to preantral follicle stage (Period 2) but was significantly associated with O3 exposure during Periods 1, 2, and 3. These observations were robust in the dual-pollutant model considering co-exposure to PM2.5 and O3. The results indicated an inverse association between ovarian reserve and ambient O3 exposure and suggested distinct susceptibility windows for O3 and PM2.5 for reduced ovarian reserve. These findings highlight the need to control ambient air pollution to reduce invisible risks to women's fertility, especially at high O3 concentrations.

Transcriptome analysis of meiotic and post-meiotic spermatogenic cells reveals the potential hub genes of aging on the decline of male fertility.

Genetic and epigenetic changes in sperm caused by male aging may be essential factors affecting semen parameters, but the effects and specific molecular mechanisms of aging on male reproduction have not been fully clarified. In this study, to explore the effect of aging on male fertility and seek the potential molecular etiology, we performed high-throughput RNA-sequencing in isolated spermatogenic cells, including pachytene spermatocytes (marked by the completion of chromosome synapsis) and round spermatids (produced by the separation of sister chromatids) from the elderly and the young men. Functional enrichment analysis of differentially expressed genes (DEGs) in round spermatids between the elderly and young showed that they were significantly enriched in gamete generation, spindle assembly, and cilium movement involved in cell motility. In addition, the expression levels of DEGs in round spermatids (post-meiotic cells) were found to be more susceptible to age. Furthermore, ten genes (AURKA, CCNB1, CDC20, CCNB2, KIF2C, KIAA0101, NR5A1, PLK1, PTTG1, RAD51AP1) were identified to be the hub genes involved in the regulation of sperm quality in the elderly through Protein-Protein Interaction (PPI) network construction and measuring semantic among GO terms and gene products. Our data provide aging-related molecular alterations in meiotic and post-meiotic spermatogenic cells, and the information gained from this study may explain the abnormal aging-related male fertility decline.

Associations among post-partum rumen fill and motility, subclinical ketosis and fertility in Holstein dairy cows.

This prospective observational study aimed to investigate the association of rumen fill and motility in post-partum Holstein cows with their future reproductive performance and subclinical ketosis (SCK). The study population consisted of two independent data sets: the first (DS1) included 237 cows from 6 herds and the second one (DS2) 709 cows from 9 herds. Rumen Fill Score (RFS) was transformed into a 3 level-trait, representing very low, low and adequate dry matter intake, respectively. A binary Rumen Contraction Score (RCS) was defined as: 0: <2 contractions/2 min, impaired rumen motility and 1: ≥2 contractions/2 min, normal rumen motility. A combined binary trait based on RFS and RCS (RFCS) was also established, representing unsatisfactory and satisfactory rumen function. Three SCK traits were defined, based on 3 different thresholds, SCK_I: BHB≥1,000 mmol/L, SCK_II: BHB≥1,100 mmol/L and SCK_III: BHB≥1,200 mmol/L. Scores were assessed and blood samples collected on day 7 (DS1) or day 8 (DS2), postpartum. Kaplan-Meier survival analysis, multivariable Cox proportional hazards models and Generalized Linear Mixed Models were performed to evaluate the association of rumen and SCK traits with reproduction. Herd, parity, calving season and several postparturient diseases were also included as potential explanatory variables. Mean days from calving to pregnancy after the 1st artificial insemination (AI) and from calving to pregnancy (all AIs) were shorter for levels of rumen traits representing adequate DMI and normal rumen motility; in most cases these differences were statistically significant in both datasets. Cows with adequate DMI and normal rumen motility (only in DS2) had greater hazard (hazard ratio [HR] = 1.84 and 1.61, for RFS and RFCS, respectively) and odds (odds ratio [OR] = 2.49 and 1.98, for RFS and RFCS, respectively) for pregnancy at 1st AI. Assessment of the association of examined rumen traits with hazard and odds for pregnancy at all AIs yielded statistically significant results in both datasets. For RFS, RCS and RFCS, HRs ranged from 1.57 to 3.31 and ORs from 1.95 to 4.83. No statistically significant associations with hazard and odds for pregnancy at 1st or all AIs were detected, for any of the 3 SCK traits, in either dataset. Overall, the combined RFCS trait constantly identified more than twice the number of cows with future reproductive problems than a positive SCK blood test.

Cerium oxide nanoparticles improve the post-thaw quality and in-vivo fertility of Beetal buck spermatozoa.

The motility, health quality, and membrane disorders of spermatozoa are adversely affected during the process of semen cryopreservation due to the over-production of reactive oxygen species (ROS). Cerium oxide nanoparticles (CeO2NPs) possess properties to scavenge ROS either by mimicking specific antioxidants or by enhancing the activities of antioxidant enzymes. Therefore, we aimed at evaluating the effects of adding the CeO2NPs in the TRIS-citrate-yolk extender on in-vitro antioxidant enzyme activities, spermatozoa quality attributes, and in-vivo fertility of post-thaw Beetal buck spermatozoa. The CeO2NPs were prepared and characterized (UV-spectrophotometry, FTIR, and XRD). Semen samples, collected from bucks (n = 5), were distributed into five aliquots and diluted in an extender containing increasing concentrations of nanoparticles (0 µg/ml, called the control group, 25 µg/mL, 50 µg/mL, 75 µg/mL, and 100 µg/mL). At post-thaw, spermatozoa were evaluated for the above-mentioned attributes and the pregnancy rate by inseminating Beetal does (n = 252). Results demonstrated that CeO2NPs mitigated the detrimental effects of cryopreservation as ROS production and lipid peroxidation were lower (P < 0.001) in the 25, 50, and 75 µg/mL CeO2NPs-added groups compared to the control and 100 µg/ml CeO2NPs-added group. The addition of 25 µg/mL CeO2NPs improved (P < 0.001) the activities of superoxide dismutase, catalase, and peroxidase and the concentration of reduced glutathione (P < 0.001) compared to the other groups. In terms of sperm kinematics and velocity parameters, the groups added with the 25 and 50 µg/mL CeO2NPs exhibited higher total motility (P < 0.001), sperm progressive motility (P = 0.003), and rapid velocity (P < 0.001). The group added with the 50 µg/mL CeO2NPs had the highest (P = 0.04) average path velocity. The groups added with the 25 and 50 µg/mL CeO2NPs also exhibited higher plasma membrane integrity (P = 0.003), acrosomal integrity, and viability (P < 0.001) compared to the control group. The DNA integrity was also higher (P < 0.001) in all the CeO2NPs-added groups. The pregnancy rate was higher (P = 0.003) in the 25 (51.92 %) and 50 µg/mL CeO2NPs (58.33 %) groups compared to the other groups. Conclusively, our findings suggest that the inclusion of cerium oxide nanoparticles in the TRIS-citrate-yolk freezing extender can reduce the occurrence of cryopreservation-induced damages to Beetal's buck spermatozoa and ultimately enhance the pregnancy rate in does.

Coevolutionary stability of host-symbiont systems with mixed-mode transmission.

The coevolution of hosts and symbionts based on virulence and mode of transmission is a complex and diverse biological phenomenon. We introduced a conceptual model to study the stable coexistence and coevolution of an obligate symbiont (mutualist or parasite) with mixed-mode transmission and its host. Using an age-structured Leslie model for the host, we demonstrated how the obligate symbiont could modify the host's life history traits (survival and fecundity) and the long-term growth rate of the infected lineage. When the symbiont is vertically transmitted, we found that the host and its symbiont could maximize the infected lineage's evolutionary success (multi-level selection). Our model showed that symbionts' effect on host longevity and reproduction might differ, even be opposing, and their net effect might often be counterintuitive. The evolutionary stability of the ecologically stable coexistence was analyzed in the framework of coevolutionary dynamics. Moreover, we found conditions for the ecological and evolutionary stability of the resident host-symbiont pair, which does not allow invasion by rare mutants (each mutant dies out by ecological selection). We concluded that, within the context of our simplified model conditions, a host-symbiont system with mixed-mode transmission is evolutionarily stable unconditionally only if the host can maximize the Malthusian parameters of the infected and non-infected lineages using the same strategy. Finally, we performed a game-theoretical analysis of our selection situation and compared two stability definitions.

Breast cancer and pregnancy: From an obstetrician's perspective / Cáncer de mama y embarazo: desde la perspectiva de un obstetra

The most frequent malignant tumour affecting women throughout their reproductive years is breast cancer, which is also the most often identified cancer worldwide.The purpose of this literature review is to examine current treatments for this condition, assess how they affect pregnancy outcomes, and assess how pregnancy affects the course and prognosis of the condition itself via this narrative review. (AU)

La neoplasia que afecta con mayor frecuencia a las mujeres a lo largo de su vida reproductiva es el cáncer de mama, siendo también el tipo de cáncer más identificado a nivel mundial. El objetivo de esta revisión de la literatura es examinar los tratamientos actuales para esta situación, evaluar cómo afecta a los resultados del embarazo, así como el modo en que afecta al curso y pronóstico de la enfermedad en sí, a través de esta revisión de la narrativa. (AU)

Fibroids and Fertility.

Uterine fibroids significantly impact women's reproductive health, influencing fertility potential and pregnancy outcomes. Their growth, often facilitated by hormonal influences like estrogen and progesterone, can cause considerable disruptions in the uterus, leading to symptoms and complications that impact the quality of life and reproductive prospects of women.This article provides an exhaustive discussion of uterine fibroids, including pathophysiology, their impact on endometrial function, receptivity, fertility, and pregnancy outcomes, and the management of infertility in patients with uterine fibroids. It underlines the critical role of uterine fibroids in women's reproductive health, emphasizing the importance of effective diagnosis and treatment to promote fertility and improve pregnancy outcomes.

Economics of Fertility Care.

Family building is a human right. The high cost and lack of insurance coverage associated with fertility treatments in the United States have made treatment inaccessible for many patients. The universal uptake of "add-on" services has further contributed to high out-of-pocket costs. Expansion in access to infertility care has occurred in several states through implementation of insurance mandates, and more employers are offering fertility benefits to attract and retain employees. An understanding of the economic issues shaping fertility should inform future policies aimed at promoting evidence-based practices and improving access to care in the United States.

Breaking Down Barriers: Advancing Toward Health Equity in Fertility Care for Black and Hispanic Patients.

Infertility can affect all people, regardless of race, ethnicity, or socioeconomic status. Barriers to quality fertility care include access, financial limitations, education, and social stigmas. Although racial disparities in outcomes of assisted reproductive technology can be largely attributed to the influences of systemic racism (not race), we can make changes to improve equity of care. We propose strategies in the areas of advocacy, clinical setting, community, and outcomes to address the racial disparities.

Male Factor Infertility: What Every OB/GYN Should Know.

Male factor infertility plays a role in approximately 30% of infertility cases. Various causes of male factor infertility exist including congenital, acquired, idiopathic, or environmental factors. Identifying the underlying etiology of male factor infertility is a key step toward providing appropriate counseling, effective treatment options, and improving outcomes for couples with infertility. Although the recent advances and developments in assisted reproductive technology have undoubtedly improved fertility outcomes, clinicians must understand the scope of reproductive urologists in the evaluation and treatment of male infertility to provide comprehensive counseling, appropriate referral, comprehensive evaluation, and correct surgical sperm retrieval techniques when needed.

Influence of sex composition of surviving children on childbearing intention among high fertility married women in stable union in Northwestern, Nigeria.

Achievement of Sustainable Development Goals (SDGs), especially goals one (end all forms of poverty), three (ensure healthy lives and promote well-being for all at all ages), four (ensure inclusive and equitable quality education and promote life-long learning opportunities for all) and five (achieve gender equality and empower all women and girls) might be a mirage without conscious efforts on the part of many developing countries to reduce population growth. Rapid population growth due to a high fertility rate and fertility desire may engender strife, poverty, unemployment, competition for scarce resources.The study explored the influence of sex composition of living children on childbearing intention among high fertility married women in stable union. This was a cross-sectional descriptive study and the study population were married women with high fertility in stable union. Results showed that sex composition of surviving children were significantly associated with respondents' fertility intentions. The following were the predictors of fertility intentions; sex composition of surviving children, type of family, contraceptive use, child parity, women empowerment status and others. Sex composition of surviving children was significantly associated with the fert ility intentions of the high fertility married women in stable union.

Réalisation des objectifs de développement durable (ODD), en particulier les objectifs un (mettre fin à toutes les formes de pauvreté), trois (garantir une vie saine et promouvoir le bien-être de tous à tous les âges), quatre (garantir une éducation inclusive et équitable de qualité et promouvoir tout au long de la vie opportunités d'apprentissage pour tous) et cinq (atteindre l'égalité des sexes et autonomiser toutes les femmes et les filles) pourraient être un mirage sans des efforts conscients de la part de nombreux pays en développement pour réduire la croissance démographique. Une croissance démographique rapide due à un taux de fécondité élevé et à un désir de fécondité peut engendrer des conflits, la pauvreté, le chômage et la compétition pour des ressources rares. L'étude a exploré l'influence de la composition par sexe des enfants vivants sur l'intention de procréer parmi les femmes mariées à forte fécondité en union stable. Il s'agissait d'une étude descriptive transversale et la population étudiée était composée de femmes mariées à fécondité élevée et en union stable. Les résultats ont montré que la composition par sexe des enfants survivants était significativement associée aux intentions de fécondité des répondants. Les éléments suivants étaient les prédicteurs des intentions de fécondité : composition par sexe des enfants survivants, type de famille, utilisation de contraceptifs, parité des enfants, statut d'autonomisation des femmes et autres. La composition par sexe des enfants survivants était significativement associée aux intentions de fécondité des femmes mariées à forte fécondité vivant en union stable.

Does information on age-related fertility decline and fertility policies affect university students' family and career expectations? Evidence from a randomized controlled trial.

BACKGROUND: Past research shows that young adults have poor knowledge of age-related fertility decline and that the provision of information can improve fertility knowledge. We provide university students with information on age-related fertility and fertility-related policies and investigate whether the provision of such information affects their family formation and career expectations. METHODS: A three-armed randomized controlled trial was conducted online in Singapore between September and October 2021. A total of 1000 undergraduate students were recruited through campus advertisements to complete a 30- to 45-minute online survey, which randomly exposed participants to one of three informational brochures on age-related fertility decline, fertility policies, or diabetes (control group). Participants answered questions on family formation and career expectations both before and after the information intervention. Analysis of covariance was used to assess the effects of the information intervention. RESULTS: Exposure to age-related fertility information resulted in significant reductions in the ideal age at first childbirth, significant increases in the expected probability of marriage before age 30, and (among female participants) significant increases in the expected likelihood of undergoing social egg-freezing. No difference existed in child-number ideals, educational aspirations, and income expectations between groups after exposure. No difference existed between the fertility policy information group and the control group after exposure in any of the outcomes of interest. CONCLUSIONS: Information on age-related fertility decline brought forward university students' expected timing of childbearing and marriage without reducing their educational and career expectations. The provision of fertility information at early ages, such as during university, can help correct widespread inaccurate beliefs about fertility and promote realistic family formation planning without adversely affecting educational and career goals. TRIAL REGISTRATION: ClinicalTrials.gov.

'Dysgenic fertility' is an ideological, not a scientific, concept. A Comment on: 'Stability and change in male fertility patterns by cognitive ability across 32 birth cohorts' (2023), by Bratsberg & Rogeberg.

Recently Bratsberg & Rogeberg (2023) presented an analysis in Biology Letters of how cognitive ability is associated with fertility in Norwegian men. Our concern relates to the theoretical framework of this paper. The analysis is framed around the concept of 'dysgenic fertility', which is treated throughout as a scientific theory, but 'dysgenic fertility' is not science, it is an ideological concept.

Novel axonemal protein ZMYND12 interacts with TTC29 and DNAH1, and is required for male fertility and flagellum function.

Male infertility is common and complex, presenting a wide range of heterogeneous phenotypes. Although about 50% of cases are estimated to have a genetic component, the underlying cause often remains undetermined. Here, from whole-exome sequencing on samples from 168 infertile men with asthenoteratozoospermia due to severe sperm flagellum, we identified homozygous ZMYND12 variants in four unrelated patients. In sperm cells from these individuals, immunofluorescence revealed altered localization of DNAH1, DNALI1, WDR66, and TTC29. Axonemal localization of ZMYND12 ortholog TbTAX-1 was confirmed using the Trypanosoma brucei model. RNAi knock-down of TbTAX-1 dramatically affected flagellar motility, with a phenotype similar to the sperm from men bearing homozygous ZMYND12 variants. Co-immunoprecipitation and ultrastructure expansion microscopy in T. brucei revealed TbTAX-1 to form a complex with TTC29. Comparative proteomics with samples from Trypanosoma and Ttc29 KO mice identified a third member of this complex: DNAH1. The data presented revealed that ZMYND12 is part of the same axonemal complex as TTC29 and DNAH1, which is critical for flagellum function and assembly in humans, and Trypanosoma. ZMYND12 is thus a new asthenoteratozoospermia-associated gene, bi-allelic variants of which cause severe flagellum malformations and primary male infertility.

The effect of China's many-child policy on the number of births and the prevalence of serious teratogenic and disabling defects in Hunan Province.

BACKGROUND: To research the effect of China's many-child policy on the number of births and the prevalence of serious teratogenic and disabling defects (STDDs) in Hunan province. METHODS: We performed an observational study based on the Birth Defect (BD) Surveillance System of Hunan Province and chose STDD case cards. From 2012-2022, we defined the following 4 periods: the one-child policy (OCP) (2012.01-2013.12), partial two-child policy (PTCP) (2014.1-2015.12), universal two-child policy (UTCP) (2016.1-2020.12), and the early stage of the three-child policy (ETCP) (2021.1-2022.12). Crude odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to examine the association of policy changes with STDDs. Crame'r's V was calculated to estimate the effect sizes. Joinpoint regression analysis and annual percent change (APC) were used for each segment of the trend. RESULTS: A total of 1,652,079 births were included in this analysis. Joinpoint regression analysis showed that the number of perinatal births increased from 2012 to 2017, with APC = 9.52 (95% CI: 7.2 to 11.8), and decreased from 2017 to 2022, with an APC = -10.04 (95% CI: -11.9 to -8.1). The number of mothers over 30 years old gradually increased, from 25.54% during the OCP period to 54.05% during the ETCP period (Ptrend < 0.001). With policy changes, the total prevalence of STDDs increased from 28.10 per 10,000 births during the period of OCP into 46.77 per 10,000 births during the ETCP period by 66.44%. The live birth prevalence of STDDs increased only during the ETCP period (PTCP: OR = 1.27, 95% CI: 0.99-1.24, p = 0.057, UTCP: OR = 1.22, 95% CI: 0.99-1.52, p = 0.067, ETCP: OR = 1.75, 95% CI: 1.37-2.24, p < 0.001). Over the past ten years, there was a decrease in the gestational age at diagnosis (*F = 772.520, p < 0.001), from 24.49 ± 5.65 weeks in 2012 to 20.77 ± 5.17 weeks in 2022. From 2012 to 2022, the percentage of deaths within 7 days decreased with APC = -18.85 (95% CI: -26.4- -10.5, P > 0.05). CONCLUSION: Many-child policies were associated with a moderate increase in fertility especially for women in urban areas and older women. However, they have lost the ability to control birth since 2017. The total prevalence of STDDs increased over the entire period, but the live birth prevalence increased only during the ETCP period. The gestational age at diagnosis decreased and the percentage of deaths within 7 days decreased.

[Comparison of production data and reasons for culling in Hinterwald, Vorderwald, Fleckvieh and Holstein cows from 1953 to 2021]. / Leistungsdaten und Abgangsursachen von Hinterwälder-, Vorderwälder-, Fleckvieh- und Holstein-Kühen in den Jahren 1953 bis 2021.

OBJECTIVE: The primary aim of this study was to determine the current inventory of Hinterwald cows in Baden Wurttemberg and in Switzerland. A secondary goal was to compare the production data and registered reasons for culling in Hinterwald, Vorderwald, Fleckvieh and Holstein dairy cows in order to determine possible differences between extensive and intensive husbandry practices. MATERIALS AND METHODS: The relevant breeding organization data from 1953 to 2021 were obtained and analyzed. The data of the Hinterwald Breed Association of Switzerland were also included in this study because a large population has been established in the past 40 years. RESULTS: The number of Hinterwald cows used in dairy production has markedly declined in their original area of distribution in the highland zone of the Southern Black Forest. There is reason to fear that the use of this breed on dairy farms may eventually cease. In contrast, an increase in the number of Hinterwald cattle in extensive farming for meat production was seen in the Black Forest region and in Switzerland. Compared with Vorderwald, Holstein and Fleckvieh cows, the milk yield of Hinterwald cows has not increased significantly over the last 60 years, whereas other production data including fertility parameters, udder health, feet and leg scores and longevity have been outstanding. The breed comparison also revealed that the enormous increase in milk yield in the most popular dairy breeds has occurred at the expense of an unacceptably high disease prevalence and an associated shortened lifespan, often under suboptimal husbandry conditions. CONCLUSIONS AND CLINICAL RELEVANCE: The Hinterwald breed has survived as a result of the resilience of many farmers, the financial commitment of private organizations and governmental support. The characteristics of Hinterwald cattle are excellent, making this breed an ideal choice for sustainable farming, which is a necessity in view of climate change. It would appear prudent to preserve and support this breed and others as they represent a valuable gene pool.