RESUMO
Objective.Carbon fiber (CF) is good for chronic neural recording due to the small diameter (7µm), high Young's modulus, and low electrical resistance, but most high-density carbon fiber (HDCF) arrays are manually assembled with labor-intensive procedures and limited by the accuracy and repeatability of the operator handling. A machine to automate the assembly is desired.Approach.The HDCF array assembly machine contains: (1) a roller-based CF extruder, (2) a motion system with three linear and one rotary stages, (3) an imaging system with two digital microscope cameras, and (4) a laser cutter. The roller-based extruder automatically feeds single CF as raw material. The motion system aligns the CF with the array backend then places it. The imaging system observes the relative position between the CF and the backend. The laser cutter cuts off the CF. Two image processing algorithms are implemented to align the CF with the support shanks and circuit connection pads.Main results.The machine was capable of precisely handling 6.8µm carbon fiber electrodes (CFEs). Each electrode was placed into a 12µm wide trenches in a silicon support shank. Two HDCF arrays with 16 CFEs populated on 3 mm shanks (with 80µm pitch) were fully assembled. Impedance measurements were found to be in good agreement with manual assembled arrays. One HDCF array was implanted in the motor cortex in an anesthetized rat and was able to detect single unit activity.Significance.This machine can eliminate the manual labor-intensive handling, alignment and placement of single CF during assembly, providing a proof-of-concepts towards fully automated HDCF array assembly and batch production.
Assuntos
Fenômenos Eletrofisiológicos , Ratos , Animais , Fibra de Carbono , Microeletrodos , Eletrodos Implantados , Impedância ElétricaRESUMO
A carbon-fiber plate (CFP) embedded into running shoes is a commonly applied method to improve running economy, but little is known in regard the effects of CFP design features on internal foot mechanics. This study aimed to explore how systematic changes in CFP geometrical variations (i.e., thickness and location) can alter plantar pressure and strain under the forefoot as well as metatarsal stress state through computational simulations. A foot-shoe finite element (FE) model was built and different CFP features including three thicknesses (1 mm, 2 mm, and 3 mm) and three placements (high-loaded (just below the insole), mid-loaded (in between the midsole), and low-loaded (just above the outsole)) were further modulated within the shoe sole. Simulations were conducted at the impact peak instant during forefoot strike running. Compared with the no-CFP shoe, peak plantar pressure and compressive strain under the forefoot consistently decreased when the CFP thickness increased, and the low-loaded conditions were found more effective (peak pressure decreased up to 31.91% and compressive strain decreased up to 18.61%). In terms of metatarsal stress, CFP designs resulted in varied effects and were dependent on their locations. Specifically, high-loaded CFP led to relatively higher peak metatarsal stress without the reduction trend as thickness increased (peak stress increased up to 12.91%), while low-loaded conditions showed a gradual reduction in peak stress, decreasing by 0.74%. Therefore, a low-loaded thicker CFP should be considered to achieve the pressure-relief effects of running shoes without the expense of increased metatarsal stress.
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Ossos do Metatarso , Corrida , Sapatos , Fibra de Carbono , PéRESUMO
This study deals with the development of an inexpensive and single-step sorbent manufacturing methodology for the analysis of air pollutants. Disposable carbon fibre sorbents were prepared in a few minutes using the electrothermal conditioning technique. The sorbent conditioning current and time were optimised to obtain the best extraction of benzene, toluene, ethylbenzene and xylenes (BTEX) from the air samples. After sorbent characterisation, analysis parameters affecting the BTEX extraction efficiency, such as sampling volume, humidity and sampling flow rate, were optimised for active BTEX sampling. Under optimum conditions, validation parameters such as the limit of detection (LOD), repeatability, reproducibility, and linear range were found to be 0.07-0.11 mg m - 3, 1.1%-1.8%, 5.6%-9.5% and 0.24-45 mg m - 3, respectively. Thereafter, the BTEX analysis was successfully conducted using the proposed method, with acceptable recovery values (96%-103%) in the real indoor environments.
Assuntos
Poluentes Atmosféricos , Técnicas Eletroquímicas , Poluentes Atmosféricos/análise , Poluentes Atmosféricos/química , Técnicas Eletroquímicas/métodos , Volatilização , Fibra de Carbono/química , Fibra de Carbono/ultraestrutura , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Microscopia Eletrônica de VarreduraRESUMO
Carbon fiber is one of the most widely used materials in high demand applications due to its high specific properties, however, its post-recycling properties limit its use to low performance applications. In this research, the carbon fiber recovering is examined using two methods: two-step pyrolysis and microwave-assisted thermolysis. The results indicate that the fibers recovered by pyrolysis show reduced surface and structural damage, maintaining the original mechanical properties of the fiber with losses below 5%. The fibers recovered by microwaves undergo significant surface changes that reduce their tensile strength by up to 60% and changes in their graphitic structure, increasing their degree of crystallinity by Raman index ID/IG from 1.98 to 2.86 and their amorphous degree by ID"/IG ratio from 0.411 to 1.599. Recovering fibers from microwave technique is 70% faster compared to two step pyrolysis, and provides recycled fibers with superior surface activation with the presence of polar functional groups -OH, -CO, and -CH that react with the epoxy matrix. The thermal, morphological, structural and mechanical characterizations of the recovered fibers detailed in this work provide valuable findings to evaluate their direct reuse in new composite materials.
Assuntos
Pirólise , Fibra de Carbono , Resistência à TraçãoRESUMO
In this study, the engineering-oriented three-dimensional (3D) bioanode concept was applied, demonstrating that spiral-stairs-like/rolled carbon felt (SCF/RCF) configurations achieved good performances in air-cathode microbial fuel cells (ACMFCs). With the 3D anodes, ACMFCs generated significantly higher power densities of 1535 mW/m3 (SCF) and 1800 mW/m3 (RCF), compared with that of a traditional flat carbon felt anode (FCF, 315 mW/m3). The coulombic efficiency of 15.39 % at SCF anode and 14.34 % at RCF anode also is higher than the 7.93 % at FCF anode. The 3D anode ACMFCs exhibited favorable removal of chemical oxygen demand (96 % of SCF and RCF) and total nitrogen (97 % of SCF, 99 % of RCF). Further results show that three-dimensional anode structures could enrich more electrode surface biomass and diversify the biofilm microbial communities for promoting bioelectroactivity, denitrification, and nitrification. These results demonstrate that three-dimensional anodes with active biofilm is a promising strategy for creating scalable MFCs-based wastewater treatment system.
Assuntos
Fontes de Energia Bioelétrica , Desnitrificação , Nitrogênio , Fibra de Carbono , Carbono , Eletrodos , EletricidadeRESUMO
For over 30 years, carbon fiber microelectrodes have been the gold standard for measurements related to exocytosis and more generally to the processes taking place at the synaptic level. However, this method has a low throughput and molecules can escape detection due to the featureless nature of the planar microelectrodes it uses. Here we present a new electrochemical sensor that addresses these limitations. It is based on insulated protruding volcano-shaped tips of 2 µm in diameter housing two individually addressable microelectrodes. The sensor enables volume confined and parallelizable recordings of exocytosis from adherent cells. Exocytotic releases from PC12 cells measured by amperometry on our device have quantal size in agreement with commonly admitted values but happen on a much smaller time scale; mostly within half a millisecond. We demonstrate that this faster kinetics must involve a faster vesicle fusion mechanism and is plausibly due to perturbation of the plasma membrane brought by the topography of our sensor. This suggests that exocytosis kinetics may be manipulated by the adequate substrate geometry, which opens up promising new leads of investigation in the study of synaptic processes.
Assuntos
Exocitose , Ratos , Animais , Células PC12 , Cinética , Membrana Celular , Fibra de CarbonoRESUMO
Wearable biosensors monitoring various biomarkers in sweat provide comprehensive and prompt profiling of health states at molecular levels. Cytokines existed in sweat with trace amounts play an important role in cellular activity modulation. Unfortunately, flexible and wearable biosensors for cytokine monitoring have not yet been achieved due to the limitation of membrane-based structure and sensing strategy. Herein, we develop a novel electrochemical fabric based on aptamer-functionalized carbon nanotube/graphene fibers for real-time and in situ monitoring of IL-6, a paramount cytokine biomarker for inflammation and cancer. This fabric system possesses flexibility, anti-fatigue ability and breathability for wearable applications and can apply to different body parts in various forms. Moreover, the electrochemical fabric can track other biomarkers by replacing the coupling aptamer, serving as a universal platform for sweat analysis. This fabric-based platform holds the potential to facilitate an intelligent and personalized health monitoring approach.
Assuntos
Técnicas Biossensoriais , Dispositivos Eletrônicos Vestíveis , Citocinas/análise , Fibra de Carbono , Suor/química , Biomarcadores/análise , Oligonucleotídeos/análise , Monitorização FisiológicaRESUMO
With the gradual popularity of wearable devices, the demand for high-performance flexible wearable sensors is also increasing. Flexible sensors based on the optical principle have advantages e.g. anti-electromagnetic interference, antiperspirant, inherent electrical safety, and the potential for biocompatibility. In this study, an optical waveguide sensor integrating a carbon fiber layer, fully constraining stretching deformation, partly constraining pressing deformation, and allowing bending deformation, was proposed. The sensitivity of the proposed sensor is three times higher than that of the sensor without a carbon fiber layer, and good repeatability is maintained. We also attached the proposed sensor to the upper limb to monitor grip force, and the sensor signal showed a good correlation with grip force (the R-squared of the quadratic polynomial fitting was 0.9827) and showed a linear relationship when the grip force was greater than 10N (the R-squared of the linear fitting was 0.9523). The proposed sensor has the potential for applications in recognizing the intention of human movement to help the amputees control the prostheses.
Assuntos
Dispositivos Eletrônicos Vestíveis , Humanos , Fibra de Carbono , Próteses e Implantes , Extremidade Superior , Força da MãoRESUMO
The present study aimed to evaluate a semi-wet biocathode composed of oak white charcoal and agarose gel as an alternative to the standard carbon felt biocathodes used in microbial fuel cells (MFCs). The MFC containing the oak white charcoal cathode (Oak-MFC) recorded a higher current value than that of the MFC containing a carbon felt cathode (CF-MFC). The Oak-MFC produced approximately 4.0-fold more electrons in the external circuit and 1.7-fold more methane (CH4) than the CF-MFC. A real-time PCR targeting mcrA showed that the number of methanogens adhering to the oak white charcoal cathode was approximately 15-fold that adhering to the carbon felt cathode. These results suggest that the methanogens attached to the cathode of both MFCs received electrons and CH4 was produced from carbon dioxide (CO2). Furthermore, Oak-MFC performed better than CF-MFC, thereby suggesting that oak white charcoal bound by agarose gel can be used as an alternative methanogen cathode. The propionic acid degradation rate of Oak-MFC was faster than that of CF-MFC suggesting that the cathodic reaction may affect the anodic reaction. The use of oak-derived electrode as a methanogen cathode also could contribute to sustainable forest management and promote regular thinning of oak trees. Further, its use will enable carbon fixation and efficient energy conversion from CO2 to CH4, thus contributing to sustainable energy use.
Assuntos
Fontes de Energia Bioelétrica , Quercus , Eletricidade , Carvão Vegetal , Dióxido de Carbono , Fibra de Carbono , Sefarose , EletrodosRESUMO
The increase in anthropogenic pollution raises serious concerns regarding contamination of water bodies and aquatic species with potential implications on human health. Pharmaceutical compounds are a type of contaminants of emerging concern that are increasingly consumed and, thus, being frequently found in the aquatic environment. In this sense, an electrochemical sensor based on an unmodified and untreated carbon fiber paper (CPS-carbon paper sensor) was simply employed for the analysis of trimethoprim antibiotic in fish samples. First, the analytical conditions were thoroughly optimized in order for the CPS to achieve maximum performance in trimethoprim determination. Therefore, an electrolyte (0.1 M Britton-Robinson buffer) pH of 7 was selected and for square wave voltammetry parameters, optimum values of amplitude, frequency and step potential corresponded to 0.02 V, 50 Hz, and 0.015 V, respectively, whereas the deposition of analyte occurred at +0.7 V for 60 s. In these optimum conditions, the obtained liner range (0.05 to 2 µM), sensitivity (48.8 µA µM-1 cm-2), and LOD (0.065 µM) competes favorably with the commonly used GCE-based sensors or BDD electrodes that employ nanostructuration or are more expensive. The CPS was then applied for trimethoprim determination in fish samples after employing a solid phase extraction procedure based on QuEChERS salts, resulting in recoveries of 105.9 ± 1.8% by the standard addition method.
Assuntos
Antibacterianos , Trimetoprima , Humanos , Fibra de Carbono , Eletrodos , Carbono/química , Técnicas EletroquímicasRESUMO
Polychlorodibenzo-p-dioxins (PCDDs), polychlorodibenzofurans (PCDFs), and polychlorobiphenyls (PCBs) are semi-volatile compounds and can be partitioned in the atmosphere between the gas and particulate phase, due to their physicochemical properties. For this reason, the reference standard methods for air sampling include a quartz fiber filter (QFF) for the particulate and a polyurethane foam (PUF) cartridge for the vapor phase, and it is the classical and most popular sampling method in the air. Despite the presence of the two adsorbing media, this method cannot be used for the study of the gas-particulate distribution, but only for a total quantification. This study presents the results and the performance aim to validate an activated carbon fiber (ACF) filter for the sampling of PCDD/Fs and dioxin-like PCBs (dl-PCBs) using laboratory and field tests. The specificity, precision, and accuracy of the ACF in relation to the QFF + PUF were evaluated through the isotopic dilution technique, the recovery rates, and the standard deviations. Then the ACF performance was assessed on real samples, in a naturally contaminated area, through parallel sampling with the reference method (QFF + PUF). The QA/QC was defined according to the standard methods ISO 16000-13 and -14 and EPA TO4A and 9A. Data confirmed that ACF meets the requirements for the quantification of native POPs compounds in atmospheric and indoor samples. In addition, ACF provided accuracy and precision comparable to those offered by standard reference methods using QFF + PUF, but with significant savings in terms of time and costs.
Assuntos
Dioxinas , Bifenilos Policlorados , Dibenzodioxinas Policloradas , Dibenzodioxinas Policloradas/análise , Bifenilos Policlorados/análise , Carvão Vegetal/análise , Dibenzofuranos/análise , Fibra de Carbono , Dioxinas/análise , Poeira/análise , Dibenzofuranos Policlorados/análise , Monitoramento Ambiental/métodosRESUMO
The widespread heavy metal pollution endangers human health; hence, accurate on-site detection and quantification of heavy metal content in the surroundings is a vital step in reversing the harmful effect. Herein, an electrochemical sensor based on flexible cerium metal-organic framework@multi-walled carbon nanotubes/carbon cloth (CeMOF@MWCNTs/CC) was constructed for simultaneous on-site detection of Cd2+ and Pb2+ in food and water samples. The rich carboxyl groups of MWCNTs provided abundant sites for the adsorption of Cd2+ and Pb2+, and the mutual conversion of Ce3+ and Ce4+ in CeMOF facilitated the reduction and reoxidation of metal ions. The prepared electrode showed excellent performance in the simultaneous measurement of Cd2+ and Pb2+, with detection limits of 2.2 ppb and 0.64 ppb, respectively. More importantly, the sensing platform has been successfully used to detect simultaneously Cd2+ and Pb2+ in grain and water samples, and the detection results were consistent with the standard methods, showing great potential in environmental monitoring and food safety.
Assuntos
Cério , Estruturas Metalorgânicas , Metais Pesados , Nanotubos de Carbono , Humanos , Cádmio/química , Fibra de Carbono , Chumbo , Estruturas Metalorgânicas/química , Metais Pesados/química , Nanotubos de Carbono/química , Água , Análise de Alimentos , AlimentosRESUMO
In this study, MIL-53(Fe) was innovatively incorporated into carbon felt (CF) by growing in-situ using the solvothermal method. MIL-53(Fe)@carbon felt (MIL-53(Fe)@CF) was prepared and used for the degradation of rhodamine B (RhB). As a new photocatalytic membrane, MIL-53(Fe)@CF photocatalytic membrane has the characteristics of high degradation efficiency and recyclability. Influence of various parameters including MIL-53(Fe)@CF loading, light, electron trapper type, and starting pH on RhB degradation were investigated. The morphology, structure, and degradation properties of MIL-53(Fe)@CF photocatalytic membrane were characterized. Corresponding reaction mechanisms were explored. The results indicated that pH at 4.5 and 1 mmol/L H2O2, 150 mg MIL-53(Fe)@CF could photocatalytically degrade 1 mg/L RhB by 98.8% within 120 min, and the reaction rate constant (k) could reach 0.03635 min-1. The clearance rate of RhB decreased by only 2.8% after three operations. MIL-53(Fe)@CF photocatalytic membrane was found to be stable.
Assuntos
Fibra de Carbono , Rodaminas , Carbono , Fibra de Carbono/química , Peróxido de Hidrogênio/química , Rodaminas/química , Processos FotoquímicosRESUMO
Solar drying represents an attractive way to implement an efficient and green development strategy. The viability of open sorption thermal energy storage (OSTES) can compensate for the inherent shortcomings of intermittency and instability of solar energy for ensuring the continuity of the drying process. Nevertheless, the existing solar-powered OSTES technologies only allow a batch mode while being severely restricted by sunlight's availability, thereby heavily limiting the flexibility in managing OSTES on demand. Herein, a novel proof-of-concept that a standalone solar dryer integrated with a reversible solid-gas OSTES unit is presented. Using in situ electrothermal heating (in situ ETH) could rapidly release adsorbed water of activated carbon fibers (ACFs) in an energy-efficient manner to achieve a charging process with faster kinetics. Applying electrical power by a photovoltaic (PV) module, particularly during sunlight-absent or insufficient time, allowed multiple OSTES cycles to proceed. Moreover, ACFs cylindrical cartridges can be flexibly interconnected in either series or parallel, forming universal assemblies with well-controlled in situ ETH capacity. The mass storage density of ACFs with a water sorption capacity of 570 mg/g is 0.24 kW·h·kg-1. The desorption efficiencies of ACFs are higher than 90%, corresponding to 0.057 kW·h maximum energy consumption. The resulting prototype can diminish the fluctuation of air humidity along the night and provide a relatively steady and lower air humidity for the drying chamber. The energy-exergy and environment analysis of the drying section for both setups are estimated, respectively.
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Arctium , Energia Solar , Luz Solar , Fibra de Carbono , Carvão VegetalRESUMO
Accurate dopamine (DA) monitoring with high stability is essential for investigating the chemical basis of brain function and pathology. Electrochemical-based tissue-implantable carbon fiber electrodes (CFEs) show great potential in sensing the dynamics of neurochemicals at a sub-second timescale. However, their anti-fouling property, selectivity, and stability pose challenges. Here, we presented a novel strategy to enhance electrode biocompatibility and stability by modifying CFE with a chitosan (CS) film, brain cell membrane (M), and aptamer cholesterol amphiphiles (DNA-cho). We found that CFE was uniformly covered by a cicada-like membrane after being modified. Electrochemical characterizations indicated that DNA-cho-M-CS-CFE exhibited a wide linear range of DA concentration and showed high sensitivity, specificity, and stability. The electrode also presented excellent fouling resistance and biocompatibility. Moreover, the biosensor was used to detect DA in K+-induced brain slices and PC12 cells with a satisfactory stability and sensitivity and to prove that LPS treatment leads to the delayed and decreased release of DA. DNA-cho-M-CS-CFE showed excellent electrochemical performance and unique advantages for long-term in vivo sensing of living cells, thus providing a new feasible scheme for studying neurochemical kinetics and brain diseases.
Assuntos
Técnicas Biossensoriais , Ratos , Animais , Fibra de Carbono , Eletrodos , Dopamina/química , Encéfalo/metabolismo , Membrana Celular , Neurônios , Técnicas EletroquímicasRESUMO
BACKGROUND: The Ilizarov fixator is a popular device for treating arthrodesis of the ankle joint in complex situations. However, the therapy can fail, with nonunion or partial union that might not be load stable. There is the possibility of follow-up surgery or extended wearing of the fixator. Full weight bearing with a carbon orthosis remains another treatment option, which has not yet been investigated.The aim of the study was to determine the rate of progress that can be obtained with a carbon orthosis in cases of partial union or nonunion after fixator removal. METHODS: In this retrospective observational study thirty-three patients received a carbon orthosis after fixator removal due to nonunion or partial union. All patients were allowed to walk with the orthosis under full load. The consolidation rate was determined radiologically and compared with the imaging data obtained during the last follow-up. In addition to demographic data, the Foot and Ankle Ability Measure and pain using a numeric rating scale were determined. Nine patients had to be excluded due to insufficient follow-up, and finally n = 24 patients were included in the study. RESULTS: The average duration of fixator use was 21 weeks (range 15-40 weeks), and the total average follow-up after removal of the fixator was 16 months (range 4- 56). For 14 (58.33%) patients, there was a further increase in consolidation with the orthosis after the fixator was removed. CONCLUSION: The results show that if there is only partial union or nonunion, further consolidations can be achieved after the application of a carbon orthosis. In a difficult patient population, using an orthosis should therefore be attempted to avoid unnecessary revision operations.
Assuntos
Tornozelo , Técnica de Ilizarov , Humanos , Fibra de Carbono , Fixadores Externos , Aparelhos Ortopédicos , Articulação do Tornozelo/cirurgia , Artrodese/métodos , Estudos Retrospectivos , Resultado do TratamentoRESUMO
Adsorbents featuring abundant binding sites and high affinity to phosphate have been used to resolve water eutrophication. However, most of the developed adsorbents were focused on improving the adsorption ability of phosphate but ignored the effect of biofouling on the adsorption process especially used in the eutrophic water body. Herein, a novel MOF-supported carbon fibers (CFs) membrane with high regeneration and antifouling capability, was prepared by in-situ synthesis of well-dispersed MOF on CFs membrane, to remove phosphate from algae-rich water. The hybrid UiO-66-(OH)2@Fe2O3@CFs membrane exhibits a maximum adsorption capacity of 333.3 mg g-1 (pH 7.0) and excellent selectivity for phosphate sorption over coexisting ions. Moreover, the Fe2O3 nanoparticles anchored on the surface of UiO-66-(OH)2 through 'phenol-Fe(III)' reaction can endow the membrane with the robust photo-Fenton catalytic activity, which improves long-term reusability even under algae-rich condition. After 4 times photo-Fenton regenerations, the regeneration efficiency of the membrane could remain 92.2%, higher than that of hydraulic cleaning (52.6%). Moreover, the growth of C. pyrenoidosa was significantly reduced by 45.8% within 20 days via metabolism inhibition due to membrane-induced P-deficient conditions. Hence, the developed UiO-66-(OH)2@Fe2O3@CFs membrane holds significant prospects for large-scale application in phosphate sequestration of eutrophic water bodies.
Assuntos
Água , Humanos , Fibra de Carbono , Compostos Férricos/química , Fosfatos , AdsorçãoRESUMO
Methylene blue (MB) acted as a stabilizer for preventing surface-induced denaturation of tyrosinase (TYR) adsorbed on a carbon felt (CF) surface, which is based on shield and anchor roles preventing the unfavorable conformational change of TYR on the hydrophobic CF surface. Furthermore, MB acted as an effective adhesive for TYR immobilization on CF. The resulting TYR and MB coadsorbed CF (TYR/MB-CF) worked as an excellent working electrode unit in an electrochemical detector in a flow injection amperometric biosensor, which allowed highly sensitive consecutive determination of not only TYR substrates but also competitive inhibitors. Simultaneous adsorption of TYR and MB from their mixed solution was much useful as compared with step-wise separated adsorption of TYR on the MB-adsorbed CF, which suggests that the binding interaction of MB with TYR in the solution phase is important for this phenomenon. Fluorescence and UV-vis spectroscopy revealed that not only electrostatic forces between the cationic MB and anionic amino acid residues of TYR but also hydrophobic interactions via the phenothiazine ring of MB play a principal binding driving force of MB with TYR at the surface of the TYR molecules. Synchronous fluorescence, three-dimensional fluorescence, and circular dichroism (CD) spectroscopy clarified that the conformation and the secondary structure of TYR slightly changed upon the MB binding, implying that MB binding leads to the modification of the original intramolecular bonding in part.
Assuntos
Técnicas Biossensoriais , Carbono , Carbono/química , Fibra de Carbono , Monofenol Mono-Oxigenase/química , Azul de Metileno , Fenóis , Técnicas Biossensoriais/métodosRESUMO
The widely applied aromatic nitration in modern industry leads to toxic p-nitrophenol (PNP) in environment. Exploring its efficient degradation routes is of great interests. In this study, a novel four-step sequential modification procedure was developed to increase the specific surface area, functional group, hydrophilicity, and conductivity of carbon felt (CF). The implementation of the modified CF promoted reductive PNP biodegradation, attaining 95.2 ± 0.8% of removal efficiency with less accumulation of highly toxic organic intermediates (e.g., p-aminophenol), compared to carrier-free and CF-packed biosystems. The constructed anaerobic-aerobic process with modified CF in 219-d continuous operation achieved further removal of carbon and nitrogen containing intermediates and partial mineralization of PNP. The modified CF promoted the secretion of extracellular polymeric substances (EPS) and cytochrome c (Cyt c), which were essential components to facilitate direct interspecies electron transfer (DIET). Synergistic relationship was deduced that glucose was converted into volatile fatty acids by fermenters (e.g., Longilinea and Syntrophobacter), which donated electrons to the PNP degraders (e.g., Bacteroidetes_vadinHA17) through DIET channels (CF, Cyt c, EPS) to complete PNP removal. This study proposes a novel strategy using engineered conductive material to enhance the DIET process for efficient and sustainable PNP bioremediation.
Assuntos
Carbono , Elétrons , Fibra de Carbono , Biodegradação Ambiental , Nitrofenóis/metabolismoRESUMO
PURPOSE: Impending and actual pathologic fractures secondary to metastatic bone disease, lymphoma, or multiple myeloma often require intramedullary fixation followed by radiation therapy. Because of carbon's low atomic number, there are reduced computed tomography (CT) imaging artifacts and dose perturbation when planning postoperative radiation for carbon fiber (CF) rods. Herein, we characterize the dosimetric properties of CF implants compared with titanium alloy (TA) for proton and photon. METHODS AND MATERIALS: TA and CF samples were acquired from an implant manufacturer. Material characteristics were evaluated by CT scans with and without metal artifact reduction (MAR). Relative stopping power (RSP) was determined from the range pull-back of each sample in a 20-cm range proton beam. Photon transmission measurements were made in a solid water phantom and compared with the modeled dosimetry from the RayStation planning system. RESULTS: CF caused no visible CT artifacts, and MAR was not necessary for Hounsfield unit (HU) determination (median, 364 HU) or contouring, whereas TA (median, 3071 HU) caused substantial artifacts, which were improved, but not eliminated by MAR. The proton RSP was measured as 3.204 for TA and 1.414 for CF. For 6 MV photons, the measured transmission was 89.3% for TA and 98% for CF. CF RSP calculation and transmission from CT HU showed a physical density overestimate compared with measurements, which would cause a slight, but acceptable, dose uncertainty (<10% proton range or 1% photon transmission). CONCLUSIONS: With a density similar to bone, CF implants did not cause imaging artifacts and minimal dose perturbation compared with TA. Although the CF proton RSP is underestimated and the photon attenuation is overestimated by the HU, both effects are relatively small and may be most easily accounted for by planning with a 2-mm expansion around organs at risk beyond or in close proximity to the implant.
