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1.
Braz Oral Res ; 36: e129, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36383835

RESUMO

This systematic review evaluated the potential utility of platelet-rich fibrin (PRF) in bone repair in animals. The question is: can the use of PRF in bone defects in healthy rats induce bone repair compared to clot? This systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (Prisma). The protocol was registered with Prospero (CRD [42020162319]). The literature search involved nine databases, including grey literature. All studies evaluated the bone defects created in rats filled with PRF and clots (control). Biomaterial evaluation was also performed in this study. The risk of bias was assessed using the Systematic Review Center for Laboratory Animal Experimentation (Syrcle) tool for animal studies. A meta-analysis of quantitative data was performed to estimate the effect of PRF on bone repair in rats. Heterogeneity among the studies was assessed using the I2 statistic. The literature search retrieved 685 studies, 10 of which fulfilled the eligibility criteria, and 4 were included in the quantitative assessment. Analysis of the risk of bias revealed that most studies had a high risk of bias in performance and detection. Meta-analysis yielded divergent results and the absence of a statistically significant effect: PRF with control (standardized mean difference 2.54, 95% confidence interval -0.80-5.89; p = 0.14). In general, study heterogeneity was high (I2 ≥ 75.0%). The quality of the studies that influenced the conclusion of the review was based on the PICO, the sources and form of the search, the study selection criteria, the form of evaluation of publication bias, the evaluation of the quality of the studies, and data extraction by two researchers. PRF did not provide significant benefits for bone repair, resulting in unpredictable effects.


Assuntos
Fibrina Rica em Plaquetas , Procedimentos Cirúrgicos Reconstrutivos , Ratos , Animais
2.
Int J Implant Dent ; 8(1): 39, 2022 10 03.
Artigo em Inglês | MEDLINE | ID: mdl-36184700

RESUMO

PURPOSE: To compare the release of platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-I) and interleukin 1ß (IL-1ß) of plasma rich in growth factors (PRGF) and leucocyte platelet-rich fibrin (L-PRF) and to evaluate their biological implication in osteoblasts. METHODS: Blood from 3 healthy volunteers was processed into PRGF, immediate L-PRF (L-PRF 0') and L-PRF 30 min after collection (L-PRF-30') and a control group. Growth factors release were analyzed at 7 times by ELISA. Cell proliferation, collagen-I synthesis and alkaline phosphatase activity were assessed in primary cultures of human osteoblasts. RESULTS: A slower controlled release of IGF-I, VEGF and PDGF was observed in the PRGF group at day 14. A higher synthesis of type I collagen was also quantified in PRGF. L-PRF released significantly higher amounts of IL-1ß, that was almost absent in the PRGF. CONCLUSIONS: The addition of leukocytes dramatically increases the secretion of proinflammatory cytokines, which are likely to negatively influence the synthesis of type I collagen and alkaline phosphatase (ALP) by osteoblasts.


Assuntos
Fibrina Rica em Plaquetas , Fosfatase Alcalina/metabolismo , Colágeno Tipo I/metabolismo , Citocinas/metabolismo , Preparações de Ação Retardada/metabolismo , Fibrina/metabolismo , Humanos , Fator de Crescimento Insulin-Like I/metabolismo , Interleucina-1beta/metabolismo , Leucócitos , Osteoblastos/metabolismo , Fator de Crescimento Derivado de Plaquetas/metabolismo , Fibrina Rica em Plaquetas/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo
3.
Int J Periodontics Restorative Dent ; 42(6): e209-e216, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36305938

RESUMO

A lack of bone volume may compromise a correct three-dimensional implant placement. This study was designed to evaluate the clinical and radiographic outcomes of simultaneous horizontal guided bone regeneration (GBR) performed using autogenous bone or blood-derived products mixed with a bone xenograft. The study population consisted of patients operated on using one of two clinical protocols for GBR: group A, which used autogenous bone mixed with a bone xenograft, and group B, which used advanced platelet-rich fibrin (A-PRF) mixed with a bone xenograft. The primary outcome was the clinical gain in the peri-implant defect. The secondary outcomes included an analysis of the postoperative healing, periodontal parameters, marginal bone loss, and occurrence of adverse events. All of the surgeries were carried out successfully. One patient in each group experienced a case of early implant loss, and three patients (one in group A and two in group B) presented biologic complications. The mean peri-implant vertical defect heights at baseline in group A and group B were 3.6 ± 0.9 mm and 4 ± 1.5 mm, respectively (P = .382). No statistically significant differences in the mean residual defect heights (P = .521) or in the postoperative wound healing (P = .611) were observed. Stable peri-implant marginal bone levels were recorded after loading in both groups. The use of A-PRF combined with a particulate bone xenograft and covered with a fixed collagen membrane may provide clinical results similar to those obtained via autogenous bone mixed with bone xenograft.


Assuntos
Produtos Biológicos , Implantes Dentários , Fibrina Rica em Plaquetas , Humanos , Bovinos , Animais , Regeneração Tecidual Guiada Periodontal/métodos , Implantação Dentária Endóssea/métodos , Estudos Retrospectivos , Regeneração Óssea , Minerais/uso terapêutico
4.
Pan Afr Med J ; 42: 154, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36187047

RESUMO

Introduction: for the treatment of varicose ulcer performed clinical and laboratory reasoning of the use of ultrasonic-assisted venous ulcer debridement and Platelet-rich plasma with radiofrequency ablation in an outpatient setting, was carried out. Methods: ultrasonic-assisted debridement of trophic ulcer were performed for 50 patients with lower extremity varicose veins at decompensation stage. The effectiveness of ultrasonic-assisted debridement was evaluated by indicators of bacteriological, morphological, cytological study and assessment of trophic ulcers according to the MEASURE system. After ultrasonic-assisted debridement, the patients were divided into two groups: 30 patients, who underwent combined Platelet-rich plasma to stimulate wound regeneration and 20 patients, for whom the Granuflex hydrocolloid bandage was applied for the same purpose. Results: a comparative analysis of ulcer regeneration in two groups of patients proved that in cases of platelet rich plasma the time of transition from inflammatory-regenerative type to regenerative one is much shorter than when using a hydrocolloid dressing. In 28 patients undergoing Platelet-rich plasma (PRP) and Platelet-rich fibrin (PRF), the radiofrequency ablation of the principal superficial and perforating veins was performed. Another 22 patients performed autodermoplasty of trophic ulcers after radiofrequency ablation. Conclusion: our experience has shown that in a one-day inpatient surgical clinic such a multidisciplinary approach to treatment of venous ulcers, including ultrasonic-assisted debridement that is stimulation of wounded process by Platelet-rich plasma with further surgeries to remove the causes of decompensated chronic insufficiency, is promising regarding low costs of treatment and rehabilitation of these patients.


Assuntos
Ablação por Cateter , Fibrina Rica em Plaquetas , Plasma Rico em Plaquetas , Úlcera Varicosa , Varizes , Desbridamento , Humanos , Extremidade Inferior/cirurgia , Úlcera , Ultrassom , Úlcera Varicosa/etiologia , Úlcera Varicosa/cirurgia , Varizes/complicações , Varizes/cirurgia , Cicatrização
5.
Sci Rep ; 12(1): 17426, 2022 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-36261500

RESUMO

Platelet-rich fibrin (PRF) is a hot research topic because of its regenerative effect in humans. However, data reporting about its application in companion animals is lacking. The study aimed to supplement currently available data on PRF cell composition in canine patients by isolating peripheral blood mononuclear cells (PBMC), namely T cells, matured B cells, monocytes and macrophages, and adapting current protocols of cell flow cytometry for PRF analysis. The canine patient population was divided into three subgroups: animals with periodontitis only, animals with neoplasia and periodontitis, and healthy controls. Individual clinical parameters of the patients and evaluation of the wound healing quality were included in the research. In the present study, canine PRF cell composition was analyzed for the first-time using cell flow cytometry protocol. A higher proportion of PBMC cells related to wound healing (CD3+, CD3+ CD4+ CD8-, CD14+) were found in the PRF of control, periodontitis and neoplasia groups compared to the respective blood samples, which implies a positive outcome associated with clinical PRF usage in canine patients. Proportions of monocytes and macrophages were higher in PRF samples compared to the blood of healthy patients and periodontitis-affected patients. However, inflammatory and neoplastic processes do not affect the distribution of PBMC.


Assuntos
Fibrina Rica em Plaquetas , Humanos , Cães , Animais , Leucócitos Mononucleares , Contagem de Leucócitos , Leucócitos , Doença Crônica
6.
Medicina (Kaunas) ; 58(10)2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36295487

RESUMO

Background and objectives: The study aimed to evaluate and compare the amount of papillary gain and black triangle height reduction after intervention with a microtunnelling technique with either Connective tissue graft (CTG) or Platelet-rich fibrin (PRF) as a biomatrix at 6 months using a microsurgical approach. Materials and Methods: Twenty-six patients with interdental papillary loss were included in the study. The patients were selected randomly for the study groups with thirteen patients in each group: a control group where CTG was utilised as a matrix, and a test group where PRF was utilised as a matrix, for interdental papillary reconstruction. A microtunnelling technique was performed for both the study groups under a surgical microscope. The primary parameters assessed were interdental Papillary height (PH) and Black triangle height (BTH) at baseline, with secondary parameters Visual analogue score by dentist (VAS-D) and patient (VAS-P) assessed at 6 months. Results: Both the control and test groups showed a significant reduction in BTH within their respective group at six months (p < 0.05). The gain in papillary height significantly improved only in the CTG group at 6 months. However, significant differences could not be demonstrated for any of the variables such as BTH (p value = 0.582) and PH (p-value = 0.892) between the study groups at 6 months. Conclusions: IDP reconstruction utilising a microtunnelling approach with CTG or PRF was successful without any significant differences between the groups for the parameters assessed at 6 months.


Assuntos
Retração Gengival , Inosina Difosfato , Fibrina Rica em Plaquetas , Humanos , Retração Gengival/cirurgia , Tecido Conjuntivo/transplante , Transplante Autólogo
7.
Artigo em Inglês | MEDLINE | ID: mdl-36231859

RESUMO

Platelet-rich fibrin (PRF), a second-generation platelet concentrate, has been widely recognized in numerous studies for its performance of wound healing and regeneration in dentistry. However, bibliometric analysis of PRF in dentistry is still scarce. Thus, this study aimed to conduct and delineate a bibliometric analysis of the application of PRF and its changing trend in dentistry. All papers concerning PRF up to 30 June 2022 were included in the literature search from the Web of Science Core Collection database. These data were then entered into Microsoft Excel, analyzed by the SAS statistical software, and visualized by the VOSviewer software. A total of 562 articles were retrieved following the exclusive criteria. The results demonstrated that the trend of annual publication increased continually (p for trend < 0.01), more notably in the last five years. The majority of the articles were original (58.01%), followed by reviews (17.08%), and case reports (10.14%). The three major study designs were clinical studies (20.11%), randomized controlled trials (17.62%), and review/meta-analysis (17.08%). PRF was most frequently employed in oral surgery (31.14%), periodontal regeneration (22.42%), and implant therapy (18.68%). Turkey (16.19%), India (12.28%) and China (7.12%) were the top 3 countries publishing PRF studies. By comparing the total number of publications, total citation counts and varying counting methods, a combination of numerous counting methods was suggested for use since each counting method yields different results. Taken together, we hope with these informative findings, researchers could focus on the future direction or advancement in PRF, laying a foundation for evidence-based dentistry.


Assuntos
Fibrina Rica em Plaquetas , Cirurgia Bucal , Bibliometria , China , Cicatrização
8.
Int Orthod ; 20(4): 100681, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36151016

RESUMO

OBJECTIVES: Primary objective: To evaluate the effect of L-PRF on the rate of maxillary canine retraction. SECONDARY OBJECTIVE: To validate the rate of maxillary canine retraction by identifying the levels of Interleukin-1ß (IL-1ß) and Tumour Necrosis Factor-α (TNF-α) in the Gingival Crevicular Fluid (GCF). MATERIALS AND METHOD: Fifteen females (age range, 18-25 years) with Class I bimaxillary dentoalveolar protrusion malocclusions were included. After levelling and alignment of maxillary arches, 1st premolars were extracted from both sides. Canines were retracted immediately after the extraction of 1st premolars in control sides and placement of L-PRF plugs in the experimental sides. The amount of canine retraction was evaluated from study models recorded before the extraction of 1st premolars (T0) and at 1-week (T1), 2-weeks (T2), 4-weeks (T3), and 8-weeks (T4) after the beginning of canine retraction by using digital model superimpositions. The concentrations of IL-1ß and TNF-α were evaluated from the GCF collected at T0, T1, T2, T3, and T4 by using commercially available human IL-1ß and TNF-α ELISA kits. RESULTS: The amount of canine retraction in the experimental sides was statistically greater during T0-T1 (P=0.038),T1-T2 (P=0.002), and T2-T3 (P=0.011) periods. Total canine retraction during T0-T4 was statistically greater in experimental sides (2.43±0.46mm) than control sides (2.08±0.28mm) (P=0.001). The concentration of IL-1ß increased significantly in the experimental sides at T1 (P=0.000),T2 (P=0.000), and T3 (P=0.032). The concentration of TNF-α increased both in the control and experimental sides, but it was statistically greater in the experimental sides compared to control sides at T1 (P=0.000) and T2 (P=0.006). A positive but not statistically significant correlation was noted between the rate of canine retraction and the concentrations of cytokines such as IL-1ß and TNF-α in GCF. CONCLUSION: Over an 8-week period, autologous L-PRF statistically accelerated the rate of maxillary canine retraction, but only by 0.35mm. This small acceleration occurred mainly in the first 4 weeks. During the following 4 weeks, the amount of canine retraction was comparable on the experimental and control sides.


Assuntos
Líquido do Sulco Gengival , Fibrina Rica em Plaquetas , Feminino , Humanos , Adolescente , Adulto Jovem , Adulto , Técnicas de Movimentação Dentária , Fator de Necrose Tumoral alfa , Boca , Biomarcadores , Leucócitos
9.
Int J Implant Dent ; 8(1): 36, 2022 09 13.
Artigo em Inglês | MEDLINE | ID: mdl-36098849

RESUMO

PURPOSE: Platelet-rich fibrin (PRF) has been proposed as promising biomaterials with the advantages of host accumulation of platelets and leukocytes with entrapment of growth factors and fibrin scaffold. However, limitations including fast resorption rate (~ 2 weeks) restricts its clinical application. Recent studies have demonstrated heating treatment can prolong PRF degradation. Current published articles used the method of 75 °C for 10 min to obtain longer degradation, while few studies investigated the most suitable temperature for heating horizontal PRF. Our present study was to discover and confirm the optimum temperature for heat treatment before obtaining H-PRF gels by investigating their structure, mechanical properties, and bioactivity of the H-PRF gels after heating treatment. METHODS: In the present study, 2-mL upper layer of horizontal PRF was collected and heated at 45 °C, 60 °C, 75 °C, and 90 °C to heat 2-mL upper layer of horizontal PRF for 10 min before mixing with the 2-mL lower layer horizontal PRF. The weight, solidification time and the degradation properties were subsequently recorded. Scanning electron microscopy (SEM) and rheologic tests were carried out to investigate the microstructure and rheologic properties of each H-PRF gel. The biological activity of each H-PRF gel was also evaluated using live/dead staining. RESULTS: H-PRF gel prepared at 75 °C for 10 min had the fast solidification period (over a tenfold increase than control) as well as the best resistance to degradation. The number of living cells in H-PRF gel is greater than 90%. SEM showed that H-PRF gel becomes denser as the heating temperature increases, and rheologic tests also revealed that the heat treatment improved the mechanical properties of H-PRF gels when compared to non-heated control group. Future clinical studies are needed to further support the clinical application of H-PRF gels in tissue regeneration procedures. CONCLUSIONS: Our results demonstrated that the H-PRF gel obtained at 75 °C for 10 min could produce a uniform, moldable gel with a short time for solidification time, great rheologic behavior and, high percent of live cells in PRF gel. A promising use of the commonly utilized PRF gel was achieved facilitating tissue regeneration and preventing degradation.


Assuntos
Fibrina , Fibrina Rica em Plaquetas , Plaquetas , Fibrina/análise , Géis/análise , Calefação , Fibrina Rica em Plaquetas/química , Temperatura
10.
Med. oral patol. oral cir. bucal (Internet) ; 27(5): e468-e475, September 01, 2022. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-209814

RESUMO

Background: We aimed to histomorphometrically evaluate the effects of Leucocyte-Platelet-Rich Fibrin (L-PRF),with and without the combination of a bone grafting material, for alveolar ridge preservation using an in vivocanine model.Material and Methods: Seven dogs (Female Beagles, ~18-month-old) were acquired for the study. L-PRF wasprepared from each individual animal by drawing venous blood and spinning them through a centrifuge at 408RCF-clot (IntrasSpin, Intra-Lock, Boca Raton, FL). L-PRF membranes were obtained from XPression fabrication kit (Biohorizons Implant Systems, Inc., AL, USA). A split mouth approach was adopted with the first molarmesial and distal socket defects treated in an interpolated fashion of the following study groups: 1) Empty socket (negative control); 2) OSS filled defect 3) L-PRF membrane; and 4) Mix of Bio-Oss® with L-PRF. After six weeks,samples were harvested, histologically processed, and evaluated for bone area fraction occupancy (BAFO), vertical/horizontal ridge dimensions (VRD and HRD, respectively), and area of coronal soft tissue infiltration.Results: BAFO was statistically lower for the control group in comparison to all treatment groups. Defects treatedwith Bio-Oss® were not statistically different then defects treated solely with L-PRF. Collapsed across all groups,L-PRF exhibited higher degrees of BAFO than groups without L-PRF. Defects filled with Bio-Oss® and Bio-Oss®with L-PRF demonstrated greater maintenance of VRD relative to the control group. Collapsed across all groups,Bio-Oss® maintained the VRD and resulted in less area of coronal soft tissue infiltration compared to the emptydefect. Soft tissue infiltration observed at the coronal area was not statistically different among defects filled withL-PRF, Bio-Oss®, and Bio-Oss® with L-PRF.Conclusions: Inclusion of L-PRF to particulate xenograft did not promote additional bone heading at 6 weeks invivo. ... (AU)


Assuntos
Animais , Feminino , Cães , Processo Alveolar , Regeneração Óssea , Leucócitos , Dente Molar , Fibrina Rica em Plaquetas , Extração Dentária , Alvéolo Dental/cirurgia
11.
Rev. flum. odontol ; 3(59): 8-13, set.-dez. 2022. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1380506

RESUMO

Obter corretamente a Fibrina Rica em Plaquetas (PRF) depende da execução de cada etapa de maneira protocolada. Nesse sentido, no Laboratório Associado de Pesquisa Clínica (LPCO), da Faculdade de Odontologia da Universidade Federal Fluminense (FO-UFF) é utilizado o sistema IntraSpin® de centrifugação do sangue coletado, que caracteriza o segundo passo para preparo deste material autólogo. O objetivo do presente trabalho é realizar uma descrição prática do correto manuseio da Centrífuga IntraSpin para garantir a obtenção do PRF e sua efetiva aplicabilidade clínica.


Obtaining Platelet Rich Fibrin (PRF) correctly depends on the execution of each step in a protocol manner. In this sense, at the Associated Clinical Research Laboratory (LPCO), of the Faculty of Dentistry in the Federal Fluminense University (FO-UFF) the IntraSpin® centrifugation system is used in the collected blood, that characterizes the second step to prepare this autologous material. The objective of this present work is to make a practical description of the correct handling of the IntraSpin Centrifuge to guarantee the PRF's biocompatibility and its effective clinical applicability.


Assuntos
Centrífugas , Odontologia , Fibrina Rica em Plaquetas
12.
Rev. flum. odontol ; 3(59): 1-7, set.-dez. 2022. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1380373

RESUMO

A Fibrina Rica em Plaquetas (PRF) é caracterizada por sua abrangente aplicabilidade na Odontologia. Neste sentido, a venopunção é uma etapa fundamental para sua obtenção. Tal procedimento consiste na identificação das veias superficiais, localizadas na região da fossa antecubital dos membros superiores, para que através da utilização do sistema a vácuo de coleta seja obtido o sangue venoso do paciente. O objetivo deste trabalho é realizar um guia prático abordando cada etapa que compreende a coleta sanguínea para produção do PRF permitindo sua reprodutibilidade de forma segura e eficiente.


Platelet Rich Fibrin (PRF) is characterized by its wide applicability in Dentistry. In this sense, venipuncture is a fundamental step towards obtaining it. Such procedure consists of the identification of superficial veins, located in the region of the antecubital fossa of the upper limbs, so that through the use of the vacuum collection system, the patient's venous blood is obtained. The objective of this work is to carry out a practical guide covering each step that comprises the blood collection for the production of the PRF allowing its reproducibility in a safe and efficient way.


Assuntos
Veias , Coleta de Amostras Sanguíneas , Odontologia , Fibrina Rica em Plaquetas
13.
F1000Res ; 11: 29, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36101858

RESUMO

Background: Tissue engineering technology has been used globally and proven to accelerate wound healing. This study aimed to analyse the effect of adding hydroxyapatite (HA) as a scaffold to platelet-rich fibrin (PRF) as a growth factor in accelerating the wound healing process as seen from the expression of matrix metalloproteinase-13 (MMP-13). Methods: This research is an animal experiment conducted on 18 rabbits ( Oryctolagus cuniculus). Rabbits were randomly divided into the following three groups of treatment: (G1) the application of PRF group, (G2) the application of PRF+HA group and (C) the control group without any application. Furthermore, each treatment group was split randomly into three groups of observation time. Periodontal tissue biopsy was performed to analyse the histopathological features that were examined on the basis of the level of MMP-13 immunoexpression. Results: MMP-13 immunoexpression in the PRF+HA group showed better histoscore results, indicating a substantial reduction in MMP-13 values compared with other groups. The healing process was shown to increase with increasing observation time (p<0.05), and the PRF+HA group outperformed the PRF and control groups. On day 3, MMP-13 exhibited a dark brown colour of Immunohistochemistry (IHC), which indicated an increase in the expression value of MMP-13 in the early stages of healing, namely, inflammation. On day 14, light brown IHC was seen, especially in group 2, as a reference that the remodeling process had begun. Conclusions: This study indicates that the application of HA can accelerate the socket healing process by decreasing the level of immunoexpression of MMP-13. HA is an alloplastic material that has inherent bioactive properties that support osteoconduction, which functions as a scaffold in the form of a fibrin matrix that can bind MMPs so that it can accelerate the wound healing process.


Assuntos
Fibrina Rica em Plaquetas , Animais , Regeneração Óssea , Durapatita/farmacologia , Metaloproteinase 13 da Matriz , Coelhos , Cicatrização
14.
BMJ Case Rep ; 15(9)2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36113960

RESUMO

Periapical lesions of endodontic origin are caused by microbial infection of pulp. According to various studies, it is known that necrosis of pulp provides a favourable habitat for microbes to replicate and release various toxins into the periapical tissue leading to inflammation and formation of a periapical lesion. A variety of non-invasive methods to manage such lesions include conservative root canal treatment, aspiration-irrigation technique, decompression technique, calcium hydroxide therapy, lesion sterilisation and tissue repair therapy, and the apexum procedure. We present a case report describing non-surgical management of a large periapical lesion associated with a permanent central incisor displaying internal inflammatory resorption using platelet rich fibrin (PRF), bone graft and mineral trioxide aggregate (MTA).


Assuntos
Hidróxido de Cálcio , Fibrina Rica em Plaquetas , Hidróxido de Cálcio/uso terapêutico , Durapatita , Humanos , Pemetrexede , Tratamento do Canal Radicular
15.
J Wound Care ; 31(9): 800-804, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36113546

RESUMO

OBJECTIVE: Platelet-rich fibrin (PRF) plays a role in promoting wound healing by releasing cytokines, chemokines and growth factors, and by inducing proliferation and activation of cells. A pressure injury (PI) is a treatable but serious and costly disease with adverse outcomes for the patient. However, traditional PI treatments are time-consuming, with limited effectiveness. Thus, we aimed to investigate the effects and mechanisms of PRF on skin wound healing in PIs in vivo in a rat model. METHOD: PRF was prepared from the blood of male Wistar rats. A rat model for PI ischaemia/reperfusion injury was established by placing a magnet onto the back skin, where a magnetic steel plate had been previously implanted. The rats were randomised into two groups: the control group was treated with sterile gauze dressings and the iPRF group received additional PRF. Skin wound healing rate was calculated and a CD31/Masson's trichrome stain performed. RESULTS: In this study, 16 rats were allocated to the two groups (n=8 in each group). PRF improved the skin wound healing rate of PIs in the rats; haematoxylin and eosin staining and CD31 staining showed that the number of capillaries increased significantly in the wound. However, Masson's trichrome staining showed no increase in fibrotic tissues after PRF treatment. CONCLUSION: In this in vivo rat model for PI, PRF accelerated skin wound healing by increasing angiogenesis in the wound.


Assuntos
Fibrina Rica em Plaquetas , Animais , Citocinas , Amarelo de Eosina-(YS)/farmacologia , Masculino , Ratos , Ratos Wistar , Aço/farmacologia , Cicatrização/fisiologia
16.
J Appl Oral Sci ; 30: e20220020, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36074433

RESUMO

BACKGROUND: Autologous platelet concentrates (APCs) are promising therapeutic agents in facial rejuvenation since they are a great source of cytokines, growth factors and other biologically active substances. Obtained from the patient's blood, they have the advantages of reducing immunological reactions, making the procedure safer, well tolerated, with minimal adverse effects and lower cost. Currently, they are used for facial rejuvenation both in combination with microneedling and in mesotherapy techniques, as well as to treat facial acne scars, melasma and wounds after laser ablative treatments. This review summarizes current knowledge on the use of APCs, ranging from basic concepts related to their composition and mechanisms of action to up-to-date information on their clinical efficacy. METHODOLOGY: MEDLINE (PubMed) was searched from inception through 2021 for English language publications on APCs for facial rejuvenation. RESULTS: A total of 100 files were found. Based on the available literature, APCs for skin rejuvenation are safe and well tolerated. The most studied product is the first-generation material, platelet-rich plasma (PRP). CONCLUSIONS: The results are in general favorable, but the quality of the studies is low. The second and third generation products, platelet-rich fibrin (PRF) and injectable platelet-rich fibrin (i-PRF), respectively, are easier to be obtained and, at least in vitro , seem to induce greater collagen production than PRP, especially under lower relative centrifugation forces, but to date only a few clinical trials evaluating these products exist. More high-quality trials with appropriate follow-up are necessary to provide adequate evidence that may help to improve the treatment regimens with APCs. Many aspects should be considered when designing clinical trials to evaluate APCs, such as the patients' characteristics that best predict a favorable response, the optimal number of sessions and the interval between them, the characteristics of the studies and the development of better instruments to evaluate skin aging.


Assuntos
Fibrina Rica em Plaquetas , Plasma Rico em Plaquetas , Envelhecimento da Pele , Face , Humanos , Rejuvenescimento
17.
J Evid Based Dent Pract ; 22(3): 101758, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-36162878

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Al-Maawi, S, Becker, K, Schwarz, F, Sader R, Ghanaati, S. Efficacy of platelet-rich fibrin in promoting the healing of extraction sockets: a systematic review. Int J Implant Dent 7, 117 (2021). https://doi.org/10.1186/s40729-021-00393-0 SOURCE OF FUNDING: This study was self-funded by the author TYPE OF STUDY/DESIGN: Systematic review (SR).


Assuntos
Fibrina Rica em Plaquetas , Humanos , Extração Dentária , Alvéolo Dental , Cicatrização
18.
Acta Chir Orthop Traumatol Cech ; 89(4): 279-285, 2022.
Artigo em Tcheco | MEDLINE | ID: mdl-36055668

RESUMO

PURPOSE OF THE STUDY It was a prospective, randomised, unblinded study. Effects of the application of platelet-rich fibrin (PRF) on the anatomic anterior ligament reconstruction were examined. MATERIAL AND METHODS A total of 40 patients who underwent a surgery between 2012 and 2014 were enrolled in the study. The patients were randomised into two groups: the group with PRF application (n=20) and the group without PRF application (n=20, control group). The study included 11 women and 29 men, with the mean age at the time of inclusion into the study 29.1 years. Vivostat ® PRF was obtained perioperatively from the patient s blood and applied intraoperatively to the hamstring tendon graft. The ACL graft healing was assessed through magnetic resonance (MRI) at 6 and 12 months postoperatively. The clinical outcomes (the Lysholm score and the IKDC Subjective score, return to pre-injury sport levels, Pivot-shift test, graft failure) as well as standardised laxity measurements using GenouRob arthrometer were performed preoperatively and 12 months after surgery. RESULTS The study was completed by thirty-three (33) patients (17 in the PRF group and 16 in the control group). In two cases, postoperative graft failure was reported (both cases in the control group). No significant differences were found in the process of graft ligamentization evaluated through MRI (p=0.07). No significant difference between the groups was identified with respect to return to pre-injury sport levels (p=0.232), or the Lysholm score and IKDC Subjective score (p=0.259, and p=0.364 respectively). The postoperative knee laxity measurement using Genourob arthrometer did not reveal any significant differences between the PRF group and the control group. DISCUSSION Results similar to those arrived at by our study have recently been published also by other authors worldwide. We can therefore assume that the application of PRF can be of benefit, especially at the early stage of healing. The results still need to be verified on a larger study group, the study design should focus on the development in the early postoperative period. CONCLUSIONS In our study group, no significant difference was observed in the evaluated subjective and objective clinical parameters. Only the parameter regarding the ACL graft failure fell just short of statistical significance, namely to the disadvantage of the group without the PRF application. Key words: anterior cruciate ligament, arthroscopy, anatomic ACL reconstruction, magnetic resonance imaging of the graft, platelet-rich fibrin, graft healing, MRI signal intensity of the graft.


Assuntos
Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior , Tendões dos Músculos Isquiotibiais , Instabilidade Articular , Fibrina Rica em Plaquetas , Lesões do Ligamento Cruzado Anterior/cirurgia , Reconstrução do Ligamento Cruzado Anterior/métodos , Feminino , Tendões dos Músculos Isquiotibiais/transplante , Humanos , Instabilidade Articular/cirurgia , Articulação do Joelho/cirurgia , Masculino , Estudos Prospectivos , Resultado do Tratamento
19.
Int J Biol Macromol ; 222(Pt A): 1605-1618, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36116591

RESUMO

Advanced platelet-rich fibrin (A-PRF) provides long-term release of growth factors that potentially accelerate wound healing. In this study, core-shell nanofibrous structure of polyvinyl alcohol (PVA) core and gelatin (Gel) shell containing A-PRF is fabricated through coaxial electrospinning method. PVA/(Gel/A-PRF) core-shell nanofibers had the highest porosity, specific surface area and hydrophilicity among all the studied nanofibers. PVA/(Gel/A-PRF) core-shell nanofibers with a tensile stress of 7.43 ± 0.38 MPa and an elastic modulus of 102.05 ± 9.36 MPa had higher mechanical properties than PVA/Gel/A-PRF and PVA/Gel blend nanofibers. PVA/(Gel/A-PRF) nanofibers had a 47.41 ± 1.97 % degradability over 7 days of immersion in PBS. The release of VEGF and PDGF-AB growth factors from PVA/(Gel/A-PRF) core-shell nanofibers and PVA/Gel/A-PRF blend nanofibers were evaluated. It was shown that L929 cell proliferation and adhesion on PVA/(Gel/A-PRF) core-shell nanofibers were significantly higher than other samples. Also, chicken chorioallantoic membrane (CAM) assay revealed that the highest angiogenic potential among the studied samples related to PVA/(Gel/A-PRF) sample. In vivo studies on a rat model showed wound closure for PVA/(Gel/A-PRF) group was 97.83 ± 2.03 % after 11 days. Histopathological and immunohistochemical examinations approved the acceleration of wound healing by PVA/(Gel/A-PRF) core-shell nanofiber dressing. The results strongly recommend the use of PVA/(Gel/A-PRF) core-shell nanofiber dressing for the repair of full-thickness wounds.


Assuntos
Nanofibras , Fibrina Rica em Plaquetas , Ratos , Animais , Nanofibras/química , Cicatrização , Bandagens , Álcool de Polivinil/química , Gelatina/química , Fibrina
20.
Int J Biol Macromol ; 222(Pt A): 1168-1174, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36179874

RESUMO

The present study evaluated the quantitative effects of platelet-rich fibrin (PRF) for the repair of extraction socket in Sprague Dawley (SD) rat model by assessing several key clinical parameters. Seventy two male SD rats were subjected to surgical extraction of the maxillary right incisor. Rats were randomly divided into four groups with eighteen rats in each group based on the treatment received: extraction socket without treatment of PRF was taken as control (group I). Extraction socket implanted with 0.1, 0.2, and 0.4 mL of PRF was taken as study groups (groups II, III, and IV). The obtained results demonstrated that, low dose of PRF efficiently enhanced the natural healing cascade. Whereas, high dose interfered with native tissue contribution and altered the natural healing process. The beneficial effects of quantity-based application of PRF may raise the possibility of a new approach as complementary therapy besides conventional treatment.


Assuntos
Fibrina Rica em Plaquetas , Masculino , Animais , Ratos , Alvéolo Dental/cirurgia , Extração Dentária , Ratos Sprague-Dawley
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