Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 867
Filtrar
1.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(1): 80-85, 2020 Feb 01.
Artigo em Chinês | MEDLINE | ID: mdl-32037771

RESUMO

The relationship between areca nut as a primary carcinogen and oral cancer has been widely concerned. Areca can change the levels of reactive oxygen species (ROS) and autophagy in cells, and the levels of ROS and autophagy are closely related to the occurrence and development of tumors. This paper reviewed the relationships among areca nut, intracellular ROS, and autophagy.


Assuntos
Autofagia , Neoplasias Bucais , Fibrose Oral Submucosa , Areca , Humanos , Nozes , Espécies Reativas de Oxigênio
2.
Braz Oral Res ; 34: e002, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049107

RESUMO

Oral potentially malignant disorders (OPMD) possess significant chances of malignancy conversion. In order to develop an early diagnostic tool, the present study evaluated the expression of miRNA-21 and 31 as salivary markers. The case-control study was carried out in 36 healthy participants as controls and in 36 patients who were newly diagnosed as OPMD having four different lesions including leucoplakia, oral sub mucous fibrosis (OSMF)궱, oral lichen planus, and (OSMF)궱 with leucoplakia. The samples were also classified as non-dysplastic, or with mild, moderate, and severe dysplasia according to their histopathological reports. The salivary miRNA-21 and 31 expressions were studied using real-time PCR. The statistical analysis was carried out using SPSS version 22. Salivary miRNA-21 (p-value = 0.02) and 31 (p-value = 0.01) were significantly upregulated in severe dysplasia compared with control. Among the different lesions, leucoplakia had significant upregulation of miRNA-21 and 31. miRNA-21 can be used as a diagnostic marker with specificity of 66% and sensitivity of 69%. The area under the ROC curve was 0.820 for miRNA-21 and 0.5 for miRNA-31, which proved that miRNA-21 is a better diagnostic marker than miRNA-31 for OPMD.


Assuntos
MicroRNAs/análise , Neoplasias Bucais/patologia , Lesões Pré-Cancerosas/patologia , Saliva/química , Análise de Variância , Biomarcadores Tumorais/análise , Estudos de Casos e Controles , Humanos , Leucoplasia Oral/patologia , Líquen Plano Bucal/patologia , Modelos Lineares , Neoplasias Bucais/química , Fibrose Oral Submucosa/patologia , Curva ROC , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Índice de Gravidade de Doença
3.
Med Oral Patol Oral Cir Bucal ; 25(1): e49-e55, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31880289

RESUMO

BACKGROUND: The objective of this study investigated the distribution of immature dendritic cells (DCs), Langerhans cells and plasmacytoid DCs in oral submucous fibrosis (OSMF), OSMF associated with oral squamous cell carcinoma (OSMF-OSCC), oral leukoplakia (OL), and oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS: Fourteen cases of OSMF, 9 of OSMF-OSCC, 8 of OL¸ 45 of OSCC and 8 of normal epithelium were retrospectively retrieved and their diagnoses confirmed. Immunoreactions against CD1a, CD207 e CD303 were performed and the number of positive cells quantified. RESULTS: A significant decrease of CD1a+ was found in OSMF (p≤0.05), OSMF-OSCC (p ≤ 0.01), and OSCC (p ≤ 0.001) when compared to normal epithelium. For CD207+ the significance decrease was observed in OSMF-OSCC (p ≤ 0.05), and OSCC (p ≤ 0.01) when compared with normal epithelium, and in OSMF when compared with OL (p ≤ 0.05). There was no significant difference for CD303, but increased in CD303+ was observed in OSCC when compared with normal epithelium. CONCLUSION: The decrease in the number of CD1a+ and CD207+ cells may be associate to the development of oral OSCC, and in OPMDs they might be indicators of malignant transformation.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Bucais , Fibrose Oral Submucosa , Antígenos CD , Humanos , Lectinas Tipo C , Leucoplasia Oral , Lectinas de Ligação a Manose , Estudos Retrospectivos
4.
J Contemp Dent Pract ; 20(8): 970-973, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31797856

RESUMO

AIM: To evaluate the salivary lactate dehydrogenase (LDH) levels in clinico-pathologically confirmed oral submucous fibrosis (OSMF), oral cancer and clinically diagnosed tobacco pouch keratosis patients. MATERIALS AND METHODS: A prospective, comparative study was carried out in a tertiary healthcare centre located in Loni from October 2013 to January 2014. A total of 120 patients were separated into 4 groups depending upon the clinical diagnosis as follows. Group I: healthy control (with no addictions and diseases). Group II: oral cancer. Group III: oral submucous fibrosis. Group IV: habitual tobacco chewers (tobacco addiction without any disease). Substantiation was done using biopsy. The samples were inspected for salivary LDH levels by the technique in line with the recommendations of the International Federation of Clinical Chemistry with the help of Erba Chem semi auto analyser. RESULTS: The mean salivary LDH levels in the control, oral cancer OSMF and habitual tobacco chewer group were 86.12 ± 7.05 IU/L, 592.09 ± 28.57 IU/L, 350.43 ± 5.90 IU/L and 125.19 ± 13.42 IU/L, respectively. Out of 4 groups, LDH activity was increased in saliva of patients with tobacco pouch keratosis, OSMF, and oral cancer consistently. Notable difference was found in the mean salivary levels of the above groups. Results were subjected to appropriate statistical analysis: one-way ANOVA, Student's unpaired t test for group-wise comparison followed by post hoc Tukey's test. CONCLUSION: We observed congruous higher levels of salivary LDH in oral precancer and cancer, and hence it could be a future marker.


Assuntos
Neoplasias Bucais , Fibrose Oral Submucosa , Biomarcadores , Humanos , L-Lactato Desidrogenase , Estudos Prospectivos , Papel (figurativo) , Tabaco
5.
Indian J Dent Res ; 30(5): 755-762, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31854369

RESUMO

Background: Oral submucous fibrosis (OSF) is an insidious, chronic, disabling disease, in which there is lack of perfusion due to reduced level of the vasculature and this is said to be responsible for the epithelial atrophy seen in OSF. The degree of vasculature of the affected mucosa and its effects on the epithelial thickness remains controversial till date. Aims: This study attempts to analyze the role of angiogenesis in OSF and its progression using vascular endothelial growth factor (VEGF) and CD34 markers. Materials and Methods: The study samples for the present study comprised of 10 cases each of early OSF, moderately advanced, advanced OSF, and 10 cases of normal oral mucosa were used as controls. All the cases were subjected to immunohistochemical staining with VEGF and CD34 markers. Results: Among the different grades of OSF, we did not find any noticeable difference in VEGF expression although we found a upregulation in microvessel density (CD34) in early and moderately advanced OSF followed by a downregulation in advanced OSF. Conclusions: As the disease progresses, there is an increased production of the extracellular matrix component (collagen I and II and fibronectin) and results in fibrosis. Subsequently, it leads to the reduction in the level of corium vascularity and results in hypoxia which ultimately causes reduction and constriction of the vascular channels. This sequence of events alerts us to the relevance of early disease diagnosis and management in an irreversible pathology such as OSF.


Assuntos
Fibrose Oral Submucosa , Humanos , Imuno-Histoquímica , Mucosa Bucal , Neovascularização Patológica , Fator A de Crescimento do Endotélio Vascular
6.
Indian J Pathol Microbiol ; 62(4): 523-528, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31611434

RESUMO

Background: An alarming increase in incidence of high-risk human papillomavirus (HPV) positive tumors in head and neck squamous cell carcinoma (HNSCC) by 25% and 70% in oropharyngeal HNSCC cannot be ignored. The early oncogenes of HPV, E6, and E7 play a key role in carcinogenesis. HPV associated tumors have a better clinical outcome and a favorable prognosis. The p16 expression has high concordance with other methods of HPV detection, ascertaining p16 as a surrogate marker for HPV. Objective: To assess the immunohistochemical expression of p16 in oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC) with and without coexistent OSF as a marker for high-risk HPV detection. Materials and. Methods: Tissue blocks of 70 cases including normal, OSF, OSCC with and without OSF were subjected to IHC staining with a p16INK4A monoclonal antibody. (Biogenex, San Roman). The p16 expression was noted according to percent positivity and pattern. The data were tabulated, statistically analyzed using the Chi-square test and the P value was assessed. Results: The percentage of p16 positive cells raised from normal to OSF to OSCC with and without OSF. In addition, a shift from nuclear to cytoplasmic expression from normal to OSCC was noted with a statistical significance (P < 0.001). However, no statistical significance was established with any clinicopathologic parameters except age (P = 0.012) and habits (P= 0.023). Conclusion: The presence of HPV using p16 was not detected in OSF but was positive in OSCC. Altered pattern of expression from normal to OSF to OSCC indicates promising use of p16 as a diagnostic marker.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma de Células Escamosas/genética , Genes p16 , Fibrose Oral Submucosa/genética , Adulto , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/virologia , Feminino , Humanos , Masculino , Estadiamento de Neoplasias , Fibrose Oral Submucosa/diagnóstico , Fibrose Oral Submucosa/virologia , Papillomaviridae/patogenicidade , Infecções por Papillomavirus/complicações , Inclusão em Parafina
7.
Indian J Pathol Microbiol ; 62(4): 537-543, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31611436

RESUMO

Background: Oral submucous fibrosis (OSMF), a well-recognized oral potentially malignant disorder, results due to increased collagen production and reduced collagen degradation. Aims and Objectives: To qualitatively compare the staining properties of collagen in OSMF using two special stains based on their birefringent property using polarizing microscopy. The study also assessed the distribution and orientation of collagen fibers in different grades of OSMF. Materials and Methods: A total of 73 subjects with different clinical and histopathological staging of OSMF comprised the study population. Histopathological examination was done using hematoxylin and eosin stain, Van Gieson and picrosirius red. Collagen fibers were analyzed for polarization colors, distribution, and orientation. Results: Picrosirius red stained both thick and thin collagen fibers. Irrespective of the histopathological grades reddish orange and yellowish orange were the most predominant colors. Parallel arrangement of fibers was observed when stained with Van Gieson but picrosirius red stained sections showed a majority of parallel type I fibers with perpendicular type III fibers which increased with advancement in the histopathological grade. Yellowish orange and greenish yellow fibers were predominant in the lamina propria, while reddish orange fibers were predominant in the submucosa. Conclusion: Picrosirius red was found to be a better stain. Histopathological grading and polarization colors showed no association with each other. Collagen fibers were more thickly and tightly packed in the submucosa indicating that the process of fibrosis began there. The increase in perpendicular type III fibers with advancing histopathological grades suggested their role in fibrosis.


Assuntos
Colágeno/ultraestrutura , Corantes/química , Microscopia de Polarização/métodos , Fibrose Oral Submucosa/patologia , Coloração e Rotulagem , Cor , Humanos
8.
J Investig Clin Dent ; 10(4): e12464, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31605442

RESUMO

AIM: The present study aims to evaluate the efficacy and safety of curcumin in both topical and systemic forms for management of oral submucous fibrosis in comparison with the antioxidants. METHODS: In this randomized parallel-group single-center trial, 119 patients were enrolled. Group I received antioxidants, group II received curcumin in systemic form and group III received curcumin in both systemic and topical forms. The primary outcomes assessed were interincisal mouth opening and burning sensation using a visual analog scale. The secondary outcomes were tongue protrusion and adverse reactions. The response to treatment was analyzed using ANOVA and Fisher's exact test. RESULTS: Significant improvement in mouth opening, burning sensation and tongue protrusion was observed in all groups at 12 weeks. Mean improvement in burning sensation did not show statistical difference across the groups. A significant difference between groups II and III for improvement in mouth opening and groups I and III for improvement in tongue protrusion was noted. CONCLUSION: When administrated in both systemic and topical forms together, curcumin showed better results in the management of oral submucous fibrosis as compared with the systemic form alone or antioxidants. Curcumin has the potential to emerge as an effective alternative to conventionally prescribed medications.


Assuntos
Curcumina , Fibrose Oral Submucosa , Antioxidantes , Humanos , Escala Visual Analógica
9.
J Investig Clin Dent ; 10(4): e12473, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31631564

RESUMO

AIM: The aim of the present study was to correlate the immunoexpression of alpha-smooth muscle actin (α-SMA) for myofibroblasts with the serum levels of transforming growth factor-ß1 (TGF-ß1) in oral submucous fibrosis (OSMF). METHODS: A total of 100 cases of histopathologically confirmed OSMF were assessed for α-SMA expression. Clinical data, such as age, sex, mouth opening, and habit history, were obtained for each case. Serum TGF-ß1 levels were recorded in 73 patients with the help of enzymelinked immunosorbent assay technique. RESULTS: The staining index of α-SMA increased concomitantly with higher myofibroblast count in the increasing histopathological grades of OSMF (P ≤ .05). Serum TGF-ß1 levels were highest in the intermediate grades of OSMF. Clinical parameters, such as mouth opening, cheek flexibility, and tongue protrusion, showed a direct correlation with increasing clinical grades of OSMF. CONCLUSIONS: The progressive increase in myofibroblasts from early to advanced stages suggests their potential use as markers for evaluating the severity of OSMF. Additionally, as myofibroblasts are responsible for producing a variety of factors that are involved in the fibrotic processes; they could be the key link in the pathogenesis of OSMF. Interruption of their development, recruitment, or activation could provide a unique therapeutic target for future treatment options in patients with OSMF.


Assuntos
Fibrose Oral Submucosa , Actinas , Humanos , Músculo Liso , Fator de Crescimento Transformador beta1 , Fatores de Crescimento Transformadores
10.
Tissue Cell ; 59: 82-87, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31383292

RESUMO

Abnormal epithelial stratification is a sign of oral dysplasia and hence evaluation of surface characteristics of oral epithelial region can help in detection of cancerous progression. Surface characteristics can be better visualised by Scanning Electron Microscopy (SEM) in comparison to light microscopy. In our study we have developed automated image processing algorithms i.e. Gaussian with median filtering and Gradient filtering, using MATLAB 2016b, to segment the surface characteristics i.e. the ridges and pits in the SEM images of oral tissue of normal (13 samples) and Oral Submucous Fibrosis (OSF) (36 samples) subjects. After segmentation, quantitative measurement of the parameters like area, thickness and textural features like entropy, contrast and range filter of ridges as well as area of pit and the ratio of area of ridge vs. area of pit was done. Statistical significant differences were obtained in between normal and OSF study groups for thickness (p=0.0107), entropy (p<0.00001) and contrast of ridge (p<0.00001) for Gaussian with median filtering and for all the parameters except thickness of the ridge(p=1.386), for Gradient filtering. Thus, computer aided image processing by Gradient filter followed by quantitative measurement of the surface characteristics provided precise differentiation between normal and precancerous oral condition.


Assuntos
Algoritmos , Processamento de Imagem Assistida por Computador , Microscopia Eletrônica de Varredura , Mucosa Bucal , Neoplasias Bucais , Fibrose Oral Submucosa , Lesões Pré-Cancerosas , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/metabolismo , Mucosa Bucal/ultraestrutura , Neoplasias Bucais/metabolismo , Neoplasias Bucais/ultraestrutura , Fibrose Oral Submucosa/metabolismo , Fibrose Oral Submucosa/patologia , Lesões Pré-Cancerosas/metabolismo , Lesões Pré-Cancerosas/patologia
11.
Int J Pharm ; 569: 118610, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31415875

RESUMO

The aim was to investigate the effect of constant current iontophoresis on the delivery and biodistribution of buflomedil hydrochloride (BUF) in the buccal mucosa. Quantification was by UHPLC-MS/MS; in addition to total delivery, the amounts present in the epithelia and the lamina propria (the target tissue) were also determined. Two-compartment vertical diffusion cells were used to investigate the effect of current density (0.5, 1 and 2 mA/cm2), application time (5, 10 and 20 min) and concentration (5, 10 and 20 mM) on iontophoretic delivery of BUF from aqueous solutions. In contrast to passive delivery, iontophoresis for 10 min at 1 mA/cm2 resulted in statistically equivalent transport from a 20 mM solution and a 2% HEC hydrogel (with equivalent BUF loading; 20 µmol). BUF delivery from the hydrogel using diffusion cells and a new coplanar "side-by-side" set-up was statistically equivalent (304.2 ±â€¯28.9 and 278.2 ±â€¯40.3 µg/cm2) - passive delivery was also similar. Iontophoresis (10 min at 1 mA/cm2) using a thin film (20 µmol BUF) was superior to the passive control (323.3 ±â€¯5.9 and 24.8 ±â€¯5.9 µg/cm2). Concentrations in the LP were ~700-fold > IC50 to block collagen production, potentially providing a new therapeutic strategy for oral submucous fibrosis.


Assuntos
Mucosa Esofágica/metabolismo , Hidrogéis/administração & dosagem , Iontoforese , Fibrose Oral Submucosa/tratamento farmacológico , Pirrolidinas/administração & dosagem , Vasodilatadores/administração & dosagem , Administração Cutânea , Animais , Disponibilidade Biológica , Suínos
12.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(7): 801-804, 2019 Jul 28.
Artigo em Chinês | MEDLINE | ID: mdl-31413219

RESUMO

OBJECTIVE: To evaluate therapeutic effect of combined injection of salvizanolic acid B with triamcinolone acetonide on the treatment of the soft palate with oral submucous fibrosis.
 Methods: Salvizanolic acid B combined with triamcinolone acetonide was consecutively applied once a week for 30 weeks for 33 patients with middle and later periods of oral submucous fibrosis. The response rate of color change in the soft palate and the increase of capillary vessels (determined by degree I-IV visual analog scale) were evaluated at the 12th, 24th, and 36th months after 30 weeks treatment.
 Results: Thirty-three patients were fulfilled the study without obvious adverse reactions and they were followed up for 24 months, while 31 patients were followed up for 36 months. The color change in the soft palatal mucus and the increase of capillary vessels at the 36th month follow-up after treatment were significantly better than those at the 12th month (P=0.004).
 Conclusion: Combined injection of salvizanolic acid B with triamcinolone acetonide in the treatment of the soft palate with oral submucous fibrosis is effective.


Assuntos
Fibrose Oral Submucosa , Triancinolona Acetonida/uso terapêutico , Glucocorticoides , Humanos , Fibrose Oral Submucosa/tratamento farmacológico , Palato Mole , Resultado do Tratamento
13.
Indian J Dent Res ; 30(3): 472-477, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31397427

RESUMO

Oral submucous fibrosis [OSF] is a premalignant condition characterized by inflammation and progressive fibrosis of submucosal tissue, resulting in trismus. It is associated with chewing of areca nut in betel quid. Mortality rate is significant because it transforms into oral squamous cell carcinoma at a rate of 2.3%-7.6%. The aim of this article is to share our experience in managing a case of recurrent oral submucous fibrosis with nil mouth opening by surgical excision, coronoidotomy, and reconstruction of buccal defect using bilateral inferiorly based nasolabial flap, followed by active oral physiotherapy. The patient had reached an acceptable mouth opening with no further recurrence. The patient was observed closely for any malignant transformation. Surgical excision of bands and coronoidotomy followed by reconstruction with nasolabial flaps and active physiotherapy in the postoperative period remains a good option for recurrent and advanced cases of OSF with acceptable functional and cosmetic results.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Bucais , Fibrose Oral Submucosa , Areca , Humanos , Recidiva Local de Neoplasia
14.
Medicine (Baltimore) ; 98(27): e16339, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31277186

RESUMO

BACKGROUND: This study was performed to systematically review the efficacy of Salvia miltiorrhiza injection combined with steroids in the treatment of oral submucous fibrosis. METHODS: We searched 9 databases: Web of Science, PubMed, Medline, EBSCO, Embase, The Cochrane Library, WanFang Data, the China National Knowledge Infrastructure (CNKI), and Chinese Scientific Journals Full-text Database (VIP). Randomized controlled trials were collected to study the treatment of oral submucous fibrosis by S miltiorrhiza injection combined with steroids. Each database was searched from inception to November 2018. RevMan 5.3 software was used for the meta-analysis. RESULTS: In total, 13 randomized controlled trials involving 1190 patients were included. The results of the meta-analysis showed that compared with conventional treatment, S miltiorrhiza injection combined with steroids could significantly increase the maximal mouth opening [mean difference (MD), 0.23; 95% confidence interval (CI), 0.16-0.30; P <.0001], decrease the oral mucosal lesion area (MD, -1.35; 95% CI, -2.46 to -0.25; P = .02), improve the subjective symptom burning sensation (MD, -0.77; 95% CI, -1.38 to -0.16; P = .01), and reduce adverse drug reactions (risk ratio, 0.27; 95% CI, 0.14-0.49; P <.0001). CONCLUSIONS: The results of this meta-analysis from current evidence showed that compared with conventional treatment, S miltiorrhiza injection combined with steroid could significantly improve the maximal mouth opening and the subjective symptom burning sensation as well as decrease the oral mucosal lesion area without increasing adverse effects.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Fibrose Oral Submucosa/tratamento farmacológico , Salvia miltiorrhiza , Esteroides/uso terapêutico , Humanos , Injeções
15.
J Investig Clin Dent ; 10(4): e12443, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31325233

RESUMO

AIM: The aim of this study was to investigate the expression of Ki67, CD105 and α-smooth muscle actin (α-SMA) expression in oral submucous fibrosis (OSF) and oral squamous cell carcinoma in the background of OSF (OSCC-SMF). METHODS: The study was carried out on paraffin-embedded tissues of 30 normal oral mucosa (NOM), 50 OSF cases and 105 OSCC-SMF. The immunohistochemistry was carried out to evaluate the expression of Ki67, CD105 and α-SMA antigen. RESULTS: Ki67 labelling index (LI), CD105 and α-SMA expression showed increasing trend from NOM, low-risk epithelial dysplasia (LRED), high-risk epithelial dysplasia (HRED), well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma to poorly differentiated squamous cell carcinoma. However, there was no significant difference of α-SMA expression between HRED and WDSCC. In OSCC-SMF, Ki67 LI, CD105 and α-SMA were significantly higher in advanced clinical TNM stage, metastasis and less than 3 years patient survival as compared with early clinical TNM stage, non-metastasis and 3 years or more patient survival. CONCLUSION: Ki67 LI, α-SMA and CD105 expression alone or together correspond with the disease progression model of SMF. Hence, expression of these markers can be used as a predictive marker of clinical outcome of OSCC-SMF.


Assuntos
Carcinoma de Células Escamosas , Neoplasias Bucais , Fibrose Oral Submucosa , Actinas , Biomarcadores Tumorais , Progressão da Doença , Humanos , Antígeno Ki-67 , Músculo Liso
16.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(3): 260-264, 2019 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-31218858

RESUMO

OBJECTIVE: The aim of this study was to induce oral submucous fibrosis (OSF) in Sprague-Dawley(SD) rat models by arecoline and mechanical stimulation. METHODS: Two factors factorial design was used to divide 48 rats into 8 groups (n=6). Different concentrations of arecoline (0, 0.5, 2, and 8 mg·mL⁻¹) and mechanical stimulation (with or without brush) were treated. After 16 weeks of treatment, the mouth opening was measured, the pathological changes of the buccal mucosa were observed, and the expressions of type Ⅲ collagen, transforming growth factor ß1 (TGF-ß1), and interferon-γ (IFN-γ) were detected. RESULTS: In rats with moderate and high concentrations of arecoline, typical OSF pathological features were observed in the buccal mucosa, the mouth openings were significantly reduced, and the expression levels of type Ⅲ colla-gen and TGF-ß1 were significantly increased (P<0.05). Although mechanical stimulation can increase the three indexes of mucosa (P<0.05), no pathological change and difference in the mouth opening was observed (P>0.05). CONCLUSIONS: Moderate and high concentrations of arecoline can induce OSF in SD rats, but mechanical stimulation cannot induce OSF.


Assuntos
Arecolina , Fibrose Oral Submucosa , Animais , Arecolina/farmacologia , Fibroblastos , Mucosa Bucal , Ratos , Ratos Sprague-Dawley
17.
J Contemp Dent Pract ; 20(3): 390-394, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31204333

RESUMO

AIM: Aim of this study was to assess the efficiency of different treatment modalities for oral submucous fibrosis. MATERIALS AND METHODS: Sixty patients were included in the study, which was diagnosed as stage II oral submucous fibrosis (OSMF) based on habitual history and clinical findings. Three groups were made after randomization, i.e., group 1: capsule lycopene group, group 2: capsule lycopene and injection dexamethasone, group 3: injection dexamethasone and hyaluronidase group. Symptom severity was done by visual analog scale (VAS) scoring system viz burning sensation/pain in the patients; patient satisfaction was assessed. Vernier calipers were used to measure patients' maximum mouth opening at day 1, 1st month, 2nd month, 3rd month. RESULTS: Male and female had the mean age of 28.20 ± 4.26 and 39.34 ± 2.12 in group 1, in group 2 was 27.88 ± 7.12 and 40.92 ± 7.16, in group 3 was 28.90 ± 8.69 and 40.10 ± 6.22, respectively. There was no statistically significant difference between treatment modalities based on satisfaction. On 2nd month, maximum patients with no pain were more in group 3 followed by group 2, and this was statistically significant. At a 3rd month, the maximum reduction in pain was in group 3 followed by group 2 and group 1. Mouth opening was improved in the group 3 followed by groups 2 and 1, respectively. On 3rd month statistically significant difference was observed between the study groups. CONCLUSION: The present study concludes that the treatment with dexamethasone + hyaluronidase group showed better results in improvement in mouth opening in OSMF patients than lycopene, lycopene and dexamethasone groups. Improvement in mouth opening, reduced burning sensation in OSMF patients was also shown by lycopene. Hence lycopene can be considered as a good alternative for treatment for OSMF when dexamethasone is contraindicated due to different reasons. CLINICAL SIGNIFICANCE: Any oral cavity part can be affected by OSMF including the pharynx. It is a potentially malignant disorder. So early recognition and initiation of the effective regimen for the treatment in both early and advanced cases of OSMF are necessary.


Assuntos
Fibrose Oral Submucosa , Dexametasona , Feminino , Humanos , Licopeno , Masculino
18.
Indian J Dent Res ; 30(2): 261-266, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31169160

RESUMO

Background: Oral submucous fibrosis (OSMF) commonly seen in the South Asian countries is mostly associated with the chewing of areca nut (AN). Copper content in the AN has been implicated to play a major role in the pathogenesis of OSMF. It was found that most of the AN plantations in South India commonly use a copper-based fungicide, Bordeaux mixture (BM). Objective: To evaluate the level of copper in the AN, root, and soil of the AN plantation with and without the application of BM and to correlate the role of copper in the pathogenesis of OSMF. Materials and Methods: ANs, roots, and soil were obtained from plantations located in Tanniadi, Kerala. Four areas were selected from the plantation with and without BM application. The samples were collected twice with the interval of 6 months during January 2015 and July 2015, respectively. Statistical Analysis: Arithmetic mean and standard deviation were calculated. The differences between means were calculated by paired sample t-test. Results: There was statistically significant difference in the copper content of ANs, soil, and roots from both groups (P < 0.05). Samples treated with BM showed significantly higher copper levels as compared to their counterparts in January and July 2015. Conclusions: External copper from BM and increased processing for the commercial products could collectively increase the total copper content of the commercial AN products, and this high copper concentration may be implicated to the pathogenesis and the increasing prevalence of OSMF.


Assuntos
Fibrose Oral Submucosa , Areca , Cobre , Humanos , Índia , Nozes , Prevalência
19.
J Cancer Res Ther ; 15(3): 463-469, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31169205

RESUMO

Oral submucous fibrosis (OSMF) is a chronic progressive, scarring disease affecting oral, oropharyngeal, and sometimes the esophageal mucosa. It is characterized by the progressive fibrosis of the submucosal tissue. The pathogenesis of OSMF has been directly related to the habit of chewing areca nut and its commercial preparation, which is widespread in Indian subcontinent and Southeast Asia. The areca nut has been classified as a "group one human carcinogen." Oral squamous cell carcinoma in the background of OSMF is one of the most common malignancies in South and Southeast Asian countries. Malignant transformation has been reported in 7%-12% cases of OSMF. Histopathological spectrum of OSMF includes the apparent alterations observed in the epithelium and connective tissue. Epithelial atrophy and sometimes epithelial hyperplasia with or without dysplasia are the peculiar alterations seen in the epithelium. In the connective tissue, there is extracellular matrix remodeling which results in excessive collagenization. Further cross-linking of collagen leads to hyalinization which makes the collagen resistant to proteolysis. Owing to fibrosis in the connective tissue, there is narrowing of blood vessels which further results in compromised blood supply to the local tissue milieu, that is, hypoxia. This tissue hypoxia elicits angiogenesis which may result in the malignant transformation of OSMF. Perpetual irritation of areca nut and its constituents to the oral mucosa leads to upregulation of pro-inflammatory cytokines and further juxtaepithelial inflammation. Thus, these coordinated reactions in epithelium and connective tissue leads the OSMF toward malignant transformation.


Assuntos
Fibrose Oral Submucosa/etiologia , Fibrose Oral Submucosa/metabolismo , Animais , Atrofia , Transformação Celular Neoplásica , Progressão da Doença , Suscetibilidade a Doenças , Matriz Extracelular/metabolismo , Humanos , Hiperplasia , Mucosa Bucal/metabolismo , Mucosa Bucal/patologia , Neoplasias Bucais/etiologia , Neoplasias Bucais/metabolismo , Neoplasias Bucais/patologia , Neovascularização Patológica/etiologia , Neovascularização Patológica/metabolismo , Fibrose Oral Submucosa/patologia
20.
J Cancer Res Ther ; 15(3): 620-624, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31169230

RESUMO

Background and Objectives: Quid-chewing habit is a common and old tradition in India. It causes various potentially malignant disorders. Therefore, a study was undertaken to analyze the association of various quid-chewing habit patterns and different oromucosal lesions. Materials and Methods: A cross-sectional study was conducted on 150 cases, where all the individuals selected were having quid-chewing habit and oromucosal lesions. Detailed habit history was taken through preformed questionnaire, clinical examination was done, and the lesion was subjected to incisional biopsy and confirmed histopathologically. Results: The male to female ratio of various quid-chewing habit and oromucosal lesions was 9:1. The middle aged were more commonly involved. Of the various types of quids chewed, a combination of processed betel and processed tobacco which is commercially available was used by majority of the individuals. Oral submucous fibrosis (OSMF) was seen in majority of the cases. Interpretation and Conclusion: The present study confirms the association between betel, tobacco, and various lesions such as OSMF, leukoplakia, chewer's mucosa, lichenoid reaction, and chemical burn. It also confirms the strong association of betel to OSMF and tobacco to leukoplakia.


Assuntos
Doenças da Boca/epidemiologia , Doenças da Boca/etiologia , Mucosa Bucal/patologia , Tabaco sem Fumaça/efeitos adversos , Adolescente , Adulto , Criança , Estudos Transversais , Feminino , Hábitos , Humanos , Índia/epidemiologia , Leucoplasia Oral/epidemiologia , Leucoplasia Oral/etiologia , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/epidemiologia , Neoplasias Bucais/etiologia , Fibrose Oral Submucosa/epidemiologia , Fibrose Oral Submucosa/etiologia , Prevalência , Vigilância em Saúde Pública , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA