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1.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3312-3315, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602888

RESUMO

Phytochemical investigation of the flowers of Hosta plantaginea led to isolate of one new flavonoid glycoside,plantanone C( 1) by silica gel,Sephadex LH-20,and RP-HPLC column chromatographies. Its structure was extensively determined on basis of HR-ESI-MS and NMR spectroscopic data. Compound 1 exhibited moderate antioxidant activity against DPPH radical scavenging activity,with an IC50 value of 240. 2 µmol·L~(-1).


Assuntos
Antioxidantes/isolamento & purificação , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Hosta/química , Antioxidantes/análise , Flavonoides/análise , Flores/química , Glicosídeos/análise
2.
Zhongguo Zhong Yao Za Zhi ; 44(14): 3100-3106, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31602859

RESUMO

Sugar-poison caused blood-heat is the pathological basis of many complications of diabetes. Advanced glycation end products( AGEs) are considered as the potential glycotoxic factor that can cause blood-heat. Sophorae Flos hold the effect of removing pathogenic heat from blood. In this study,chromatographic non-enzymatic glycation reaction system of bovine serum albumin( BSA)/methylglyoxal( MGO) and Sophorae Flos was established to identify active components in Sophorae Flos inhibiting AGEs formation. The HPLC was used to analyze chromatograms before and after the incubation of Sophorae Flos and methylglyoxal. Changes of chromatographic peaks of eight compounds was found. It is speculated that this change may be due to new substance produced by the reaction of active components in Sophorae Flos and methylglyoxal,and these active components may be flavonoid component rutin. Further investigation for the effects of rutin and MGO reaction( 1 ∶ 1,1 ∶ 3,3 ∶ 1) for 6 days on the formation of AGEs was performed. The results showed that the inhibition activity of rutin on AGEs production was most obvious when the reaction ratio was 1 ∶3,and the most inhibition was in 24 h and stabilized after 3 d. The product of the reaction of rutin with MGO was identified by LC-ESI-MS/MS,which indicated that the newly formed seven substances were the mono-and di-MGO adducts of rutin. This study showed that rutin is the active component on Sophorae Flos for removing pathogenic heat from blood by forming new compounds to inhibit the formation of sugar poison products,which provides reference for rational application of Sophorae Flos.


Assuntos
Medicamentos de Ervas Chinesas/análise , Flores/química , Produtos Finais de Glicação Avançada/antagonistas & inibidores , Rutina/química , Sophora/química , Cromatografia Líquida de Alta Pressão , Aldeído Pirúvico , Espectrometria de Massas em Tandem
3.
J Chromatogr A ; 1604: 460472, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31471136

RESUMO

Pomegranate flowers is an ancient medicine that has commonly been used to treat various diseases such as diabetes. However, no reports are available on the metabolic profile of pomegranate flowers in vivo. In the present study, with the aid of HPLC-Q-TOF-MS2, 67 compounds were identified in pomegranate flowers extract, including 18 ellagitannins, 14 gallic acid and galloyl derivatives, five anthocyanins and 18 flavonoids. Seven compounds were firstly identified. In vivo, 22 absorbed compounds and 35 metabolites were identified in rat biosamples (urine, feces, plasma and tissues) after orally administered with pomegranate flowers extract. This result showed that not all compounds abundant in pomegranate flowers extract could be absorbed well in plasma and tissues. This finding also suggested a potential correlation between study on metabolic profile of these compounds in vivo and study on strategy of screening bioactivity of the isolates with in vitro cell systems evaluation. Notably, mono-glucuronide conjugated metabolite of ellagitannin compound (corilagin) was firstly identified. In addition, this is first report to identify phase II conjugate metabolites of ellagitannins in vivo after oral administration of ellagitannins-rich extracts (or foods). Thus, characterizing its multiple constitution, absorption and metabolic fate of these compounds in vivo is helpful to better analyze the active components in pomegranate flowers.


Assuntos
Cromatografia Líquida de Alta Pressão , Flores/química , Espectrometria de Massas , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Polifenóis/análise , Punicaceae/química , Animais , Masculino , Polifenóis/sangue , Polifenóis/urina , Ratos
4.
J Agric Food Chem ; 67(40): 11053-11065, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31525973

RESUMO

Oilseed rape (Brassica napus L.) is the second highest yielding oil crop worldwide. In addition to being used as an edible oil and a feed for livestock, rapeseed has high ornamental value. In this study, we identified and characterized the main floral major constituents, including phenolic acids and flavonoids components, in rapeseed accessions with different-colored petals. A total of 144 constituents were identified using ultrahigh-performance liquid chromatography-HESI-mass spectrometry (UPLC-HESI-MS/MS), 57 of which were confirmed and quantified using known standards and mainly contained phenolic acids, flavonoids, and glucosinolates compounds. Most of the epicatechin, quercetin, and isorhamnetin derivates were found in red and pink petals of B. napus, while kaempferol derivates were in yellow and pale white petals. Moreover, petal-specific compounds, including a putative hydroxycinnamic acid derivative, sinapoyl malate, 1-O-sinapoyl-ß-d-glucose, feruloyl glucose, naringenin-7-O-glucoside, cyanidin-3-glucoside, cyanidin-3,5-di-O-glucoside, petunidin-3-O-ß-glucopyranoside, isorhamnetin-3-O-glucoside, kaempferol-3-O-glucoside-7-O-glucoside, quercetin-3,4'-O-di-ß-glucopyranoside, quercetin-3-O-glucoside, and delphinidin-3-O-glucoside, might contribute to a variety of petal colors in B. napus. In addition, bound phenolics were tentatively identified and contained three abundant compounds (p-coumaric acid, ferulic acid, and 8-O-4'-diferulic acid). These results provide insight into the molecular mechanisms underlying petal color and suggest strategies for breeding rapeseed with a specific petal color in the future.


Assuntos
Brassica napus/química , Flores/química , Extratos Vegetais/química , Cromatografia Líquida de Alta Pressão/métodos , Cor , Ácidos Cumáricos/química , Flavonoides/química , Hidroxibenzoatos/química , Quempferóis/química , Espectrometria de Massas em Tandem/métodos
5.
J Agric Food Chem ; 67(39): 10853-10862, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31496237

RESUMO

Leptosperin (methyl syringate ß-d-gentiobioside) is abundantly found in manuka honey, which is widely used because of its antibacterial and possible anti-inflammatory activities. The aim of this study was to examine the molecular mechanism underlying the metabolism of leptosperin. Five phytochemicals (leptosperin, methyl syringate (MSYR), glucuronate conjugate of MSYR (MSYR-GA), sulfonate conjugate of MSYR (MSYR-S), and syringic acid (SYR)) were separately incubated with HepG2 and Caco-2 cells. After incubation, we found that the concentration of MSYR decreased, whereas the concentrations of SYR, MSYR-GA, and MSYR-S increased. By profiling with inhibitors and carboxylesterases (CES1, 2), we found that the conversion from MSYR to SYR was mediated by CES1. Lipopolysaccharide-stimulated RAW264.7 cells restored MSYR-GA to MSYR possibly by the secreted ß-glucuronidase. All of the mice administered with leptosperin, MSYR, or manuka honey showed higher MSYR (13.84 ± 11.51, 14.29 ± 9.19, or 6.66 ± 2.30 nM) and SYR (1.85 ± 0.66, 6.01 ± 1.20, or 8.16 ± 3.10 nM) levels in the plasma compared with that of the vehicle controls (3.33 ± 1.45 (MSYR) and 1.85 ± 0.66 (SYR) nM). The findings of our study indicate that the unique metabolic pathways of these compounds may account for possible functionalities of manuka honey.


Assuntos
Ácido Gálico/análogos & derivados , Glicosídeos/metabolismo , Mel/análise , Animais , Células CACO-2 , Feminino , Flores/química , Ácido Gálico/análise , Ácido Gálico/metabolismo , Glicosídeos/análise , Células Hep G2 , Humanos , Leptospermum/química , Camundongos , Camundongos Endogâmicos ICR
6.
J Chem Ecol ; 45(8): 657-666, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31407199

RESUMO

The box tree moth, Cydalima perspectalis, is an invasive pest in Europe causing damage on Buxus species. In this study, we aimed to develop a "bisexual" lure to attract both female and male moths. Based on a previous screening bioassay we tested methyl salicylate, phenylacetaldehyde and eugenol as potential attractants in different combinations. The trapping results showed that both binary and ternary blends attracted male and female moths. Catches with these blends were comparable to catches with the synthetic pheromone. Subsequently we carried out single sensillum recordings, which proved the peripheral detection of the above-mentioned compounds on male and female antennae. To identify synergistic flower volatiles, which can be also attractive and can increase the trap capture, we collected flower headspace volatiles from 12 different flowering plant species. Several components of the floral scents evoked good responses from antennae of both females and males in gas chromatography-electroantennographic detection. The most active components were tentatively identified by gas chromatography coupled mass spectrometry as benzaldehyde, cis-ß-ocimene, (±)-linalool and phenethyl alcohol. These selected compounds in combination did not increase significantly the trap capture compared to the methyl salicylate- phenyacetaldehyde blend. Based on these results we discovered the first attractive blend, which was able to attract both adult male and female C. perspectalis in field conditions. These results will yield a good basis for the optimization and development of a practically usable bisexual lure against this invasive pest.


Assuntos
Comportamento Animal/efeitos dos fármacos , Mariposas/fisiologia , Feromônios/farmacologia , Animais , Eugenol/química , Eugenol/farmacologia , Feminino , Flores/química , Flores/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Controle de Insetos , Masculino , Monoterpenos/química , Monoterpenos/farmacologia , Feromônios/análise , Robinia/química , Robinia/metabolismo , Rosa/química , Rosa/metabolismo , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/farmacologia
7.
J Agric Food Chem ; 67(36): 10145-10154, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31418564

RESUMO

2-Phenylethanol (2PE) is a representative aromatic aroma compound in tea (Camellia sinensis) leaves. However, its formation in tea remains unexplored. In our study, feeding experiments of [2H8]L-phenylalanine (Phe), [2H5]phenylpyruvic acid (PPA), or (E/Z)-phenylacetaldoxime (PAOx) showed that three biosynthesis pathways for 2PE derived from L-Phe occurred in tea leaves, namely, pathway I (via phenylacetaldehyde (PAld)), pathway II (via PPA and PAld), and pathway III (via (E/Z)-PAOx and PAld). Furthermore, increasing temperature resulted in increased flux into the pathway for 2PE from L-Phe via PPA and PAld. In addition, tomato fruits and petunia flowers also contained the 2PE biosynthetic pathway from L-Phe via PPA and PAld and increasing temperatures led to increased flux into this pathway, suggesting that such a phenomenon might be common among most plants containing 2PE. This represents a characteristic example of changes in flux into the biosynthesis pathways of volatile compounds in plants in response to stresses.


Assuntos
Camellia sinensis/metabolismo , Lycopersicon esculentum/química , Petunia/química , Álcool Feniletílico/metabolismo , Vias Biossintéticas , Flores/química , Frutas/química , Folhas de Planta/metabolismo , Temperatura Ambiente
8.
Chem Biodivers ; 16(10): e1900363, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31385642

RESUMO

In this study, the metabolite profiling of three different parts of Crocus sativus L. was measured by using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTof-MS/MS). Multivariate statistical analysis was used to distinguish among the samples from different parts. A total of 54 compounds were identified in tepals, stigmas and stamens by UPLC-QTof-MS/MS. The results stated that chemical characteristics of saffron were obviously diverse in terms of the parts of flower. Through analysis, coniferin and crocin-2 were special components in stigmas when compared to tepals and stamens. The content of flavonoids was high in tepals when compared with the stigmas. The tepal of saffron may processed as a source of flavonoids in the future. The research provided the basis for the theory that only the stigma can be used as medicine.


Assuntos
Crocus/química , Flavonoides/análise , Flores/química , Metabolômica , Cromatografia Líquida de Alta Pressão , Crocus/metabolismo , Flavonoides/metabolismo , Flores/metabolismo , Espectrometria de Massas em Tandem
9.
Chem Biodivers ; 16(10): e1900371, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31464066

RESUMO

The essential oils of five Lavandula stoechas cultivars grown in Thailand were characterized for their volatile compounds using GC-FID and GC/MS methods as well as screened for antibacterial and antioxidant activities. Dried aerial parts, including flowers and stems from each cultivar, were subjected to hydrodistillation for 4 h. The essential oil yields were 0.18 %-0.82 % w/w. Of the 95 compounds detected and identified, 1,8-cineole, fenchone, and camphor were considered the major compounds. Essential oil from each cultivar demonstrated different patterns of antibacterial activity and a variety of antioxidant properties. The highest antibacterial activity, MIC=0.39 mg mL-1 , was observed from the essential oil of L. stoechas 'major' (against Klebsiella pneumoniae and Salmonella typhimurium) and the essential oil of L. stoechas 'white lavender' (against S. typhimurium). The essential oil of L. stoechas×viridis 'St. Brelade' possessed the highest antioxidant capacity, as determined by the DPPH and ABTS assays (IC50 of 67.65 and 89.26 mg mL-1 , respectively). The results indicated that some of these essential oils could be used as key ingredients in lavender oil products in Thailand to increase their therapeutic efficacy, depending on their intended application.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Klebsiella pneumoniae/efeitos dos fármacos , Lavandula/química , Óleos Voláteis/farmacologia , Salmonella typhimurium/efeitos dos fármacos , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Benzotiazóis/antagonistas & inibidores , Compostos de Bifenilo/antagonistas & inibidores , Flores/química , Lavandula/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Picratos/antagonistas & inibidores , Componentes Aéreos da Planta/química , Ácidos Sulfônicos/antagonistas & inibidores , Tailândia
10.
Molecules ; 24(13)2019 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-31288458

RESUMO

BACKGROUND/AIM: Plants play an important role in anti-cancer drug discovery, therefore, the current study aimed to evaluate the biological activity of Alpinia zerumbet (A. zerumbet) flowers. METHODS: The phytochemical and biological criteria of A. zerumbet were in vitro investigated as well as in mouse xenograft model. RESULTS: A. zerumbet extracts, specially CH2Cl2 and MeOH extracts, exhibited the highest potent anti-tumor activity against Ehrlich ascites carcinoma (EAC) cells. The most active CH2Cl2 extract was subjected to bioassay-guided fractionation leading to isolatation of the naturally occurring 5,6-dehydrokawain (DK) which was characterized by IR, MS, 1H-NMR and 13C-NMR. A. zerumbet extracts, specially MeOH and CH2Cl2 extracts, exhibited significant inhibitory activity towards tumor volume (TV). Furthermore, A. zerumbet extracts declined the high level of malonaldehyde (MDA) as well as elevated the levels of superoxide dismutase (SOD) and catalase (CAT) in liver tissue homogenate. Moreover, DK showed anti-proliferative action on different human cancer cell lines. The recorded IC50 values against breast carcinoma (MCF-7), liver carcinoma (Hep-G2) and larynx carcinoma cells (HEP-2) were 3.08, 6.8, and 8.7 µg/mL, respectively. CONCLUSION: Taken together, these findings open the door for further investigations in order to explore the potential medicinal properties of A. zerumbet.


Assuntos
Alpinia/química , Antineoplásicos Fitogênicos/química , Extratos Vegetais/química , Pironas/química , Animais , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Catalase/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Clorofórmio/química , Flores/química , Xenoenxertos , Humanos , Malondialdeído/metabolismo , Metanol/química , Camundongos , Transplante de Neoplasias , Extratos Vegetais/farmacologia , Plantas Medicinais , Pironas/farmacologia , Solventes , Superóxido Dismutase/metabolismo
11.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2156-2162, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355575

RESUMO

Ultra performance liquid chromatography coupled with time-of-flight mass spectrometry( UPLC-Q-TOF-MS/MS) method was applied to analyze the prototypes and metabolites of the effective components of Polygonum orientale in SD rat serum and urine. The separation was performed on Agilent Eclipse Plus C_(18) column( 2. 1 mm×100 mm,1. 8 µm),with 0. 1% formic acid solution( A)-acetonitrile( B) as the mobile phase for gradient elution. Mass spectrometry data of biological samples were obtained under positive and negative electrospray ion mode. By comparing chromatogram differences between blank samples and drug treatment samples,prototype components and metabolites of the effective components of P. orientale extract were identified. The results showed that 12 metabolites were detected in serum and 26 metabolites in urine( including cross-components) of rats. The main metabolic pathways included hydrogenation,hydroxylation,glucuronidation,sulfation reaction,and methylation-glucuronidation,etc. The method established in this study was reliable and effective for studying the metabolic characteristics of the effective components of P. orientale in rats,and it can provide a reference for further studies on therapeutic material basis of this herb.


Assuntos
Medicamentos de Ervas Chinesas/farmacocinética , Flores/química , Compostos Fitoquímicos/sangue , Compostos Fitoquímicos/urina , Polygonum/química , Animais , Cromatografia Líquida de Alta Pressão , Ratos , Ratos Sprague-Dawley , Espectrometria de Massas em Tandem
12.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2519-2531, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359719

RESUMO

This project is to investigate the chemical constituents of ginsenosides from the flower buds of Panax ginseng. The compounds were isolated by using a variety of chromatographic methods including Diaion HP-20,silica gel,MCI gel and semi-preparative HPLC chromatography. Their structures were identified by NMR,and MS data. As a result,32 compounds were isolated from the extract of P. ginseng flower buds,and identified as ginsenoside Rk_3( 1),ginsenoside Rh_4( 2),ginsenoside Rh_8( 3),pseudoginsenoside Rc_1( 4),ginsenoside Rc( 5),ginsenoside Rb_2( 6),ginsenoside Rg_6( 7),20( E)-ginsenoside F_4( 8),ginsenoside Rb_1( 9),vinaginsenoside R_(16)( 10),ginsenoside Rh_6( 11),vinaginsenoside R_3( 12),5,6-didehydro-ginsenoside Rd( 13),vinaginsenoside R_4( 14),vinaginsenoside R_8( 15),ginsenoside Rf( 16),notoginsenoside E( 17),ginsenoside Ⅲ( 18),3-O-ß-D-glucopyranosyl-3ß,7ß,12ß,20 S-tetrahydroxydammar-5( 6),24-diene-20-O-ß-D-glucopyranoside( 19),20( S)-ginsenoside Rg_2( 20),20( R)-ginsenoside Rg_2( 21),notoginsenoside R_2( 22),ginsenoside F_2( 23),quinquenoside I( 24),ginsenoside M_1( 25),quinquenoside L_(10)( 26),ginsenoside Rh_5( 27),ginsenoside Rg_5( 28),ginsenoside Rk_1( 29),20( R)-ginsenoside Rg_3( 30),oleanolic acid 3-O-ß-D-glucopyranosyl-( 1→2)-ß-D-( 6'-methyl ester)-glucuronopyranoside( 31) and ginsenoside MC( 32). Among them,compounds 10,12,13,15,19,22,24,31 and 32 were isolated from P. ginseng for the first time,and compound 19 was a genuine ginsenoside firstly obtained by separation and identification,with NMR data that were also reported. Compounds 1-3,7,8,23,25-30 were isolated from P. ginseng flower buds for the first time.


Assuntos
Flores/química , Ginsenosídeos/análise , Panax/química , Saponinas/análise , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética
13.
Plant Foods Hum Nutr ; 74(3): 436-442, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31321622

RESUMO

In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO). Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test. The results showed great quantitative differences in the composition of the VO morphological parts tested. Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW). Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW). Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.


Assuntos
Antioxidantes/análise , Carotenoides/análise , Nutrientes/análise , Fenóis/análise , Viburnum/química , Ácidos Carboxílicos/análise , Catequina/análise , Ácido Clorogênico/análise , Fibras na Dieta/análise , Flores/química , Frutas/química , Especificidade de Órgãos , Pectinas/análise , Casca de Planta/química , Açúcares/análise
14.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1822-1828, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342708

RESUMO

The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.


Assuntos
Antioxidantes/farmacologia , Hibiscus/química , Polissacarídeos/farmacologia , Antioxidantes/química , Flores/química , Monossacarídeos/química , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Pigmentação , Polissacarídeos/química
15.
J Chem Ecol ; 45(8): 667-672, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31313134

RESUMO

Agriotes ustulatus is an economically important click beetle in Europe. A female-produced pheromone, (E,E)-farnesyl acetate, has been identified and is used for monitoring and detecting males. More recently, a floral lure targeting females with modest, but significant, activity has been described. Based on preliminary data, we hypothesized, that similar to the effects on the congeneric A. brevis, addition of the pheromone to the floral lure should improve female A. ustulatus catches. Also, as click beetles have been reported to respond to white light, we studied possible interactions between visual and chemical cues. In field trials, the addition of the synthetic pheromone to the floral lure resulted in a dramatic increase in the number of females trapped, whereas male catches remained unaffected and equal to those in traps baited with pheromone only. A white visual cue did not influence trap catches. Maximum catches of both sexes of A. ustulatus can be achieved using the pheromone and the floral lure inside the same trap. Furthermore, the compounds can be formulated in a single polyethylene bag dispenser, making handling of the trap easier. Due to a much larger proportion of females in the catch, this improved trap may be a promising tool for semiochemical-based, environmentally sound agricultural practice against this important pest.


Assuntos
Besouros/fisiologia , Feromônios/química , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacologia , Animais , Anisóis/química , Anisóis/farmacologia , Comportamento Animal/efeitos dos fármacos , Farneseno Álcool/análogos & derivados , Farneseno Álcool/química , Farneseno Álcool/farmacologia , Feminino , Flores/química , Flores/metabolismo , Controle de Insetos , Masculino , Feromônios/farmacologia , Estereoisomerismo
16.
Chem Biodivers ; 16(8): e1900183, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31361076

RESUMO

This work describes the study of the chemical composition and bioactivity of the essential oils (EOs) of the different organs (leaves, flowers, stems and roots) from Eruca vesicaria. According to the GC and GC/MS analysis, all the EOs were dominated by erucin (4-methylthiobutyl isothiocyanate) with a percentage ranging from 17.9 % (leaves) to 98.5 % (roots). The isolated EOs were evaluated for their antioxidant (DPPH, ABTS and ß-carotene/linoleic acid), antibacterial and inhibitory property against α-amylase and α-glucosidase. Most EOs exhibited an interesting α-glucosidase and α-amylase inhibitory potential. The roots essential oil was found to be the most active with IC50 values of 0.80±0.06 and 0.11±0.01 µg mL-1 , respectively. The essential oil of roots exhibited the highest antioxidant activity (DPPH, PI=92.76±0.01 %; ABTS, PI=78.87±0.19; and ß-carotene, PI=56.1±0.01 %). The isolated oils were also tested for their antibacterial activity against two Gram-positive and three Gram-negative bacteria. Moderate results have been noted by comparison with Gentamicin used as positive control.


Assuntos
Antioxidantes/química , Brassicaceae/metabolismo , Óleos Voláteis/química , Antibacterianos/química , Antibacterianos/farmacologia , Brassicaceae/química , Flores/química , Flores/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Hipoglicemiantes/química , Óleos Voláteis/metabolismo , Óleos Voláteis/farmacologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , alfa-Glucosidases/química , alfa-Glucosidases/metabolismo
17.
Food Chem ; 299: 125120, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31288162

RESUMO

Although coffee beans have been widely studied, application of coffee flower (CF) has not been previously investigated. Here, we evaluated the use of CF for the production of bioactive compounds, melanoidins, and bio-sugars through the green process. Pressurized hot water extraction was found to be the most appropriate method for extracting bioactive compounds from CF, which contain high values of total phenolic content and have antioxidant properties. Caffeine and trigonelline were the main compounds in CF with yields of 1070.8 mg and 1092.8 mg/100 g dry weight (DW), respectively. Melanoidins were also identified and quantified in the CF extracts that is approximately 30.2% were efficiently recovered in the initial extracts of CF. Bio-sugar was also obtained from cellulase and pectinase at a 92.8% conversion rate. The aim of this study is to promote a novel approach using high amounts of CFs in the production of functional healthy foods and beverages.


Assuntos
Coffea/química , Flores/química , Compostos Fitoquímicos/metabolismo , Polímeros/metabolismo , Açúcares/metabolismo , Alcaloides/análise , Antioxidantes/análise , Cafeína/análise , Carboidratos/análise , Fenóis/análise
18.
J Photochem Photobiol B ; 197: 111536, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31326846

RESUMO

The latent utilization of biomaterials that are osteo-conducive in the advancement of healing bone fracture has fascinated extensive consideration. This work includes the synthesis of silver nanoparticles (AgNPs) with the help of a Bauhinia acuminate plant flower extract through an ecofriendly synthetic process without any use of harmful reductants. In the fabrication of AgNPs, Bauhinia acuminate plant flower extract bio constituents acts as both stabilizing and reducing agent. The studies of Fourier transform infrared (FTIR) and X-ray diffraction (XRD) techniques confirmed the formation of AgNPS. TEM images revealed that AgNPs are uniform with average particle size of 17 nm. Further, this work explored if silver nanoparticles (AgNPs) might endorse the osteogenesis and proliferation of mesenchymal stem cells (MSCs) and advance the curing of bone fractures. We also exhibited that the prepared AgNPs could promote the in -vitro osteogenic differentiation and proliferation of MSCs'. Also, the prepared AgNps could stimulate the proliferation of mMSCs at specific concentrations of 6-20 µM. Further, cell viability studies showed that AgNPs exhibited no reduction in mouse mesenchymal stem cell viability at <4 µM. Further, these results indicated the induction effects of AgNPs on osteogenic differentiation and proliferation on MSCs, as well as the advancement of meniscus injury healing.


Assuntos
Bauhinia/química , Nanopartículas Metálicas/química , Osteogênese , Extratos Vegetais/química , Prata/química , Animais , Bauhinia/metabolismo , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Flores/química , Flores/metabolismo , Consolidação da Fratura/efeitos dos fármacos , Química Verde , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Nanopartículas Metálicas/toxicidade , Camundongos , Microscopia Eletrônica de Transmissão , Osteogênese/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier
19.
Plant Foods Hum Nutr ; 74(3): 255-265, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31209704

RESUMO

Many studies have proved that bioactive components of Aloe vera have an anti-inflammatory effect and support lipid and carbohydrate metabolism, helping to maintain normal sugar and cholesterol levels in blood and normal body weight. When aloe is applied externally, it accelerates the regeneration of the damaged skin. Aloe contains antioxidants, which may increase the shelf-life and nutritional value of food; therefore, it is widely used in cosmetic, pharmaceutical and food industry. An antioxidant activity was shown for leaf's skin, flowers and gel of aloe. In this work the future of A. vera as effective antioxidants is primarily discussed and expected trends are summarised. Furthermore, the bioactive components and the health-promoting effects of A. vera are investigated.


Assuntos
Aloe/química , Antioxidantes/metabolismo , Compostos Fitoquímicos/metabolismo , Antioxidantes/farmacologia , Flores/química , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química , Folhas de Planta/química
20.
Bioelectrochemistry ; 129: 199-205, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31200249

RESUMO

The identification of plant species not only is a hobby but also has important application value in plant resources science. Traditional plant identification often relies on the experience of botanists. The infrageneric identification of plants is easily mistaken due to similarities in organ features. In this work, we propose an electrochemical method to obtain fingerprints of plant petal tissue. Fourteen species of Lycoris were used as a model for validating this methodology. Pattern and color recognition were established for visualization of electrochemical fingerprints recorded after various solvent extractions. In addition, the infrageneric relationships of these Lycoris species were deduced from the electrochemical fingerprints since the type and content of electroactive compounds in plants are controlled by genes. The results indicate that the electrochemical fingerprints of Lycoris petals are correlated with the infrageneric relationships of native Lycoris species.


Assuntos
Técnicas Eletroquímicas/métodos , Flores/classificação , Lycoris/classificação , Adsorção , Flores/química , Grafite/química , Lycoris/química , Extratos Vegetais/química , Extratos Vegetais/classificação , Solventes , Especificidade da Espécie
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