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1.
Artigo em Russo | MEDLINE | ID: mdl-36279373

RESUMO

Postthrombophlebitic syndrome (PTPS) develops in 20-50% of patients who have had deep vein thrombosis (DVT). Patients with chronic venous insufficiency (CVI) of class C4-C5 according to the CEAP clinical classification, which developed as a result of DVT of the lower extremities, including those who underwent endovascular treatment (iliac vein stenting), are subject to staged medical rehabilitation. In this regard, the development of personalized complex technologies for the sanatorium treatment of patients with PTPS is an important medical and social problem. PURPOSE OF THE STUDY: Study of clinical efficacy and identification of the mechanisms of action of a new complex of spa treatment of patients with PTPS of the lower extremities using supravenous laser radiation, low-frequency magnetotherapy, dry-air carbon dioxide baths and structured therapeutic exercises in the gym. MATERIAL AND METHODS: 60 patients with PTPS of the lower extremities (CVI C4-C5 according to CEAP) were under observation. All patients were randomly divided into 2 groups: first group (main group) included 30 patients who received a treatment, including procedures for supravascular laser blood irradiation, pulsed magnetotherapy and dry-air carbon dioxide baths, as well as structured therapeutic exercises in gym under the supervision of an exercise therapy instructor; second group (control group) included 30 patients who received standard elastic compression (compression class 2-3) while taking lymphovenotonics (a combination of diosmin and hesperidin) and therapeutic exercises in the gym. RESULTS: Against the background of the course of treatment in patients of the main group, to a greater extent than in the control group, a decrease in the clinical symptoms of the disease was noted: a more pronounced regression of edema, a decrease in heaviness in the legs, as evidenced by the data of anthropometric studies and questionnaires on the CIVIQ-2 scale. Positive dynamics in the microcirculation system (MC) was established, which was confirmed by the data of laser Doppler flowmetry. In patients with spastic-congestive type of MC, a decrease in the initially increased myogenic and neurogenic tone of arterioles was registered. There was a decreasing of stagnation in the venular link. In patients with hyperemic-congestive type of MC, the initially reduced tone of arterioles increased, which contributed to the improvement of blood flow in the capillaries. There was also a decrease in congestion in the venular link of the microvasculature. CONCLUSION: A new effective complex method for the rehabilitation of patients with PTPS has been developed, including laser exposure according to the general method, pulsed magnetotherapy and dry-air carbon dioxide baths, which have a multifocal effect on different links in the pathogenesis of PTPS.


Assuntos
Diosmina , Hesperidina , Insuficiência Venosa , Humanos , Diosmina/uso terapêutico , Hesperidina/uso terapêutico , Dióxido de Carbono/uso terapêutico , Insuficiência Venosa/terapia , Fluxometria por Laser-Doppler , Síndrome
2.
Ter Arkh ; 94(8): 957-962, 2022 Oct 12.
Artigo em Russo | MEDLINE | ID: mdl-36286975

RESUMO

AIM: To assess the state of the microvasculature, tissue metabolism and its reserve capabilities for the purpose of early intervention for the prevention of diabetic foot syndrome. MATERIALS AND METHODS: Thirty patients were examined. The patients were divided into three groups depending on the hypoglycemic therapy (metformin, empagliflozin, dapagliflozin). The comparison group consisted of 12 volunteers who did not have carbohydrate metabolism disorders. RESULTS: It was found: the HbA1c indicator significantly decreased in all three groups; significant dynamics in the state of the microvasculature was not observed in any of the three groups; in all groups, there was an improvement in tissue metabolism, however, in this case, no significant dynamics were observed in any of the 3 groups, which indicates the need for provocative tests. CONCLUSION: For this purpose, the authors recommend the method of combined use of laser Doppler flowmetry and laser fluorescence spectroscopy. This technique allows diagnosing the state of the microvasculature and tissue metabolism and its reserve capabilities, using thermal and cold tests.


Assuntos
Diabetes Mellitus , Pé Diabético , Metformina , Humanos , Pé Diabético/diagnóstico , Pé Diabético/etiologia , Pé Diabético/prevenção & controle , Microcirculação , Hemoglobina A Glicada , Fluxometria por Laser-Doppler/métodos , Hipoglicemiantes/uso terapêutico
3.
Bull Exp Biol Med ; 173(5): 665-669, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36214986

RESUMO

A new non-invasive method for assessing stability of functioning of the human regional circulatory system was developed. The method includes synchronous measurements of the level of cutaneous blood microcirculation of symmetrical areas of paired human organs by laser Doppler flowmetry and subsequent mathematical analysis of Dopplerograms using an original computational and experimental technique based on a mathematical model developed by us in the form of a system of differential equations describing experimental patterns of left-right asymmetry of oscillatory processes in the human regional circulatory system. The proposed method allows evaluating the reserve capabilities of the regional circulatory system in a patient and increases informative value of studies. This non-invasive method can be used in clinical practice to perform a personalized assessment of the stability of regional blood circulation and predict the risks of vascular pathologies.


Assuntos
Fluxometria por Laser-Doppler , Humanos , Fluxometria por Laser-Doppler/métodos , Microcirculação , Fluxo Sanguíneo Regional
4.
Annu Int Conf IEEE Eng Med Biol Soc ; 2022: 459-462, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-36086430

RESUMO

The incidence of burn injuries is higher in low-and middle-income countries, and particularly in remote areas where the access to specialized burn assessment, care and recovery is limited. Given the high costs associated with one of the most used techniques to evaluate the severity of a burn, namely laser Doppler imaging (LDI), an alternative approach could be beneficial for remote locations. This study proposes a novel approach to estimate the LDI from digital images of a burn. The approach is a pixel-wise regression model based on convolutional neural networks. To minimize the dependency on the conditions in which the images are taken, the effect of two image normalization techniques is also studied. Results indicate that the model performs satisfactorily on average, presenting low mean absolute and squared errors and high structural similarity index. While no significant differences are found when changing the normalization of the images, the performance is affected by their quality. This suggests that changes in the intensity of the images do not alter the relevant information about the wound, whereas changes in brightness, contrast and sharpness do.


Assuntos
Queimaduras , Pele , Queimaduras/diagnóstico por imagem , Diagnóstico por Imagem , Humanos , Fluxometria por Laser-Doppler/métodos , Lasers
5.
Microcirculation ; 29(6-7): e12783, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36070200

RESUMO

BACKGROUND: Previous studies have used regional cerebral blood flow (CBF) hemodynamic response to measure brain activities. In this work, we use a laser speckle contrast imaging (LSCI) apparatus to sample the CBF activation in somatosensory cortex (S1BF) with repetitive whisker stimulation. Traditionally, the CBF activations were processed by depicting the change percentage above baseline; however, it is not clear how different methods influence the detection of activations. AIMS: Thus, in this work we investigate the influence of different methods to detect activations in LSCI. MATERIALS & METHODS: First, principal component analysis (PCA) was performed to denoise the CBF signal. As the signal of the first principal component (PC1) showed the highest correlation with the S1BF CBF response curve, PC1 was used in the subsequent analyses. Then, we used fast Fourier transform (FFT) to evaluate the frequency properties of the LSCI images and the activation map was generated based on the amplitude of the central frequency. Furthermore, Pearson's correlation coefficient (C-C) analysis and a general linear model (GLM) were performed to estimate the S1BF activation based on the time series of PC1. RESULTS: We found that GLM performed better in identifying activation than C-C. Additionally, the activation maps generated by FFT were similar to those obtained by GLM. Particularly, the superficial vein and arterial vessels separated the activation region as segmented activated areas, and the regions with unresolved vessels showed a common activation for whisker stimulation. DISCUSSION AND CONCLUSION: Our research analyzed the extent to which PCA can extract meaningful information from the signal and we compared the performance for detecting brain functional activation between different methods that rely on LSCI. This can be used as a reference for LSCI researchers on choosing the best method to estimate brain activation.


Assuntos
Circulação Cerebrovascular , Imagem de Contraste de Manchas a Laser , Fluxometria por Laser-Doppler/métodos , Hemodinâmica , Veias , Fluxo Sanguíneo Regional
7.
Microvasc Res ; 144: 104422, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35970407

RESUMO

OBJECTIVE: Examine the effects of sensory nerve blockade on cutaneous post-occlusive reactive hyperemia (PORH) and local thermal hyperemia (LTH) following prolonged upper limb ischemia. MATERIALS AND METHODS: In nine males [28 years (standard deviation:6)], volar forearm skin blood flux normalized to maximum vasodilation (%SkBFmax) was assessed at control (CTRL) and sensory nerve blockade (EMLA) treated sites during the PORH response following 20-min of complete arm ischemia and during rapid LTH (33-42 °C, 1 °C·20 s-1, held for ~30-min + 20-min at 44 °C) before and after ischemia-reperfusion (IR) injury. RESULTS: EMLA increased mean [95 % confidence-interval] PORH amplitude by 21%SkBFmax ([9,33]; p = 0.003), delayed time to peak by 111 s ([40,182]; p = 0.007) and increased area under the curve by 19,462%SkBFmax·s ([11,346,27,579]; p < 0.001) compared to CTRL. For LTH, EMLA delayed onset time by 76 s ([46,106]; p < 0.001) Pre-IR and by 46 s ([27,65]; p < 0.001) Post-IR compared to CTRL. Post-IR onset time was delayed for CTRL by 26 s ([8,43]; p = 0.007), but was not different for EMLA (p > 0.050) compared to Pre-IR. EMLA delayed time to initial peak by 24 s ([4,43]; p = 0.022, Main time effect) and it attenuated the initial peak by 27%SkBFmax ([12,43]; p = 0.002) Pre-IR and by 16%SkBFmax ([3,29]; p = 0.020) post-IR compared to CTRL. Post-IR, the initial peak was not different for CTRL (p > 0.050), but it was increased by 16%SkBFmax ([5,26]; p = 0.005) for EMLA compared to Pre-IR. Neither EMLA nor IR altered the steady-state heating plateau (all p > 0.050). CONCLUSION: For the current model of IR injury, sensory nerves appear to have a negligible influence on the LTH response in non-glabrous forearm skin once vasodilation has been initiated.


Assuntos
Hiperemia , Bloqueio Nervoso , Traumatismo por Reperfusão , Humanos , Isquemia , Fluxometria por Laser-Doppler , Masculino , Microcirculação , Fluxo Sanguíneo Regional , Traumatismo por Reperfusão/prevenção & controle , Pele/irrigação sanguínea , Vasodilatação
8.
Stomatologiia (Mosk) ; 101(4): 30-33, 2022.
Artigo em Russo | MEDLINE | ID: mdl-35943497

RESUMO

The article presents the development of functional research methods in the maxillofacial region, methods of their application and the results of scientific research on the study of the neuromuscular balance of the masticatory muscles with defects and deformations in the maxillofacial region. Methods of blood circulation research (laser doppler flowmetry, ultrasound dopplerography, computer capillaroscopy) have been developed and implemented in the tissues of the maxillofacial region: periodontal, tooth pulp, in the mucous membrane of the alveolar ridge, which allowed us to study the mechanisms of disease pathogenesis and monitor the effectiveness of treatment in the maxillofacial region.


Assuntos
Processo Alveolar , Polpa Dentária , Odontologia , Humanos , Fluxometria por Laser-Doppler , Microcirculação/fisiologia
9.
Sci Rep ; 12(1): 11456, 2022 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-35794150

RESUMO

The purpose of this study is to evaluate whether monitoring the changes of skin blood flow may be effective in assessing blood perfusion during endoscopic lumbar sympathectomy (ELS) in patients with plantar hyperhidrosis. In this study, a total of 30 patients who underwent surgical treatment for plantar hyperhidrosis at the Department of Thoracic and Cardiovascular Surgery in Yonsei University Gangnam Severance Hospital, Seoul, Korea, between July 2020 and December 2020, were retrospectively analyzed. Sympathetic denervation was performed on the third lumbar ganglion, and intraoperative laser doppler flowmetry (LDF) was used to detect the lumbar sympathetic chain accurately. We observed an abrupt increase of peripheral blood flow after sympathetic denervation, and the median percent changes of perfusion unit were 173.27 (inter-quartile range, IQR 195.48) and 392.98 (IQR 597.27) for the left and right sympathectomies, respectively. This study demonstrated the efficacy of monitoring skin blood flow via LDF during ELS. This result suggests that exact detection of blood flow using LDF is essential for improving the accuracy of ELS by checking the perfusion site on the sole in patients with plantar hyperhidrosis.


Assuntos
Hiperidrose , Humanos , Hiperidrose/cirurgia , Fluxometria por Laser-Doppler , Perfusão , Estudos Retrospectivos , Simpatectomia
10.
Jpn J Ophthalmol ; 66(5): 425-433, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35788445

RESUMO

PURPOSE: To determine whether choroidal blood flow (BF) is related to visual field (VF) defect severity and progression in eyes with open-angle glaucoma (OAG). STUDY DESIGN: Retrospective and longitudinal. METHODS: This study comprised 443 eyes of 285 OAG patients who underwent laser speckle flowgraphy (LSFG), optical coherence tomography, and visual-field (VF) testing at baseline. The patients were then observed for at least 2 years and at least 5 reliable VF tests were performed. In the LSFG images, we set regions of interest at the optic nerve head (ONH) and the parapapillary choroid to obtain ONH-tissue mean blur rate (MBR) and choroidal MBR, respectively. We used univariable and multivariable linear mixed-effects models to determine clinical factors associated with choroidal MBR at baseline. We also used a linear mixed-effects model to determine the contribution of ONH-tissue MBR and choroidal MBR to baseline mean deviation (MD) and to MD slope during follow-up, adjusting for potential confounding factors, including circumpapillary retinal nerve fiber layer thickness. RESULTS: Choroidal MBR was associated with age, MD slope, and ONH-tissue MBR (ß = -0.181, P = 0.001; ß = 0.134, P = 0.002; ß = 0.096, P = 0.049, respectively). ONH-tissue MBR was associated with both MD and MD slope (ß = 0.146, P = 0.004; ß = 0.152, P = 0.009, respectively), whereas choroidal MBR was associated only with MD slope (ß = 0.147, P = 0.005). CONCLUSION: LSFG-derived choroidal MBR might be a useful biomarker to predict VF defect progression in a Japanese population.


Assuntos
Glaucoma de Ângulo Aberto , Glaucoma , Corioide/irrigação sanguínea , Glaucoma de Ângulo Aberto/diagnóstico , Humanos , Pressão Intraocular , Japão/epidemiologia , Fluxometria por Laser-Doppler/métodos , Fluxo Sanguíneo Regional/fisiologia , Estudos Retrospectivos , Tomografia de Coerência Óptica/métodos , Transtornos da Visão
11.
J Invest Surg ; 35(9): 1673-1678, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35836365

RESUMO

BACKGROUND: Dermal blood flow is crucial for wound healing and survival of flaps in dermatologic surgery. To improve flap viability in cases of compromised perfusion topical agents can easily be applied. The aim of this placebo-controlled study was to characterize changes of DBF in healthy subjects by quantitatively assessing perfusion dynamics after application of capsaicin to establish a reference for measurements at injured sites. METHODS: In 46 healthy subjects perfusion dynamics after local application with capsaicin and placebo was noninvasively assessed, determining cutaneous oxygen saturation, relative hemoglobin count and blood flow using an Oxygen-to-See device. RESULTS: A significant raise in superficial (162% p = 0.000) and deep (144%, p = 0.000) skin oxygenation after 30 min was provoked. A highly significant raise in measurements of flow and velocity was present in superficial (523%, p = 0.000) and deep (242%, p = 0.000) sites. CONCLUSION: With the introduced model applied to observe changes in parameters of dermal blood flow in healthy subjects the authors can reliably monitor effects of topically administered capsaicin. This baseline can be used as reference for further studies in the settings of endangered flap survival or critically perfused wounds as has been proven in animal studies.


Assuntos
Capsaicina , Pele , Administração Tópica , Capsaicina/farmacologia , Humanos , Fluxometria por Laser-Doppler , Microcirculação
12.
Opt Lett ; 47(12): 3083-3086, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35709056

RESUMO

To our knowledge, all existing optical coherence tomography approaches for quantifying blood flow, whether Doppler-based or decorrelation-based, analyze light that is back-scattered by moving red blood cells (RBCs). This work investigates the potential advantages of basing these measurements on light that is forward-scattered by RBCs, i.e., by looking at the signals back-scattered from below the vessel. We show experimentally that flowmetry based on forward-scattering is insensitive to vessel orientation for vessels that are approximately orthogonal to the imaging beam. We further provide proof-of-principle demonstrations of dynamic forward-scattering (DFS) flowmetry in human retinal and choroidal vessels.


Assuntos
Vasos Retinianos , Tomografia de Coerência Óptica , Velocidade do Fluxo Sanguíneo/fisiologia , Eritrócitos , Humanos , Fluxometria por Laser-Doppler/métodos , Retina , Vasos Retinianos/diagnóstico por imagem , Vasos Retinianos/fisiologia , Tomografia de Coerência Óptica/métodos
13.
BMC Ophthalmol ; 22(1): 285, 2022 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-35765019

RESUMO

BACKGROUND: To determine whether reductions in retinal and choroidal blood flow measured by laser speckle flowgraphy are detected after 125I-plaque brachytherapy for uveal melanoma. METHODS: In a cross-sectional study, retinal and choroidal blood flow were measured using laser speckle flowgraphy in 25 patients after treatment with 125I-plaque brachytherapy for uveal melanoma. Flow was analyzed in the peripapillary region by mean blur rate as well as in the entire image area with a novel superpixel-based method. Relationships between measures were determined by Spearman correlation. RESULTS: Significant decreases in laser speckle blood flow were observed in both the retinal and choroidal vascular beds of irradiated, but not fellow, eyes. Overall, 24 of 25 patients had decreased blood flow compared to their fellow eye, including 5 of the 6 patients imaged within the first 6 months following brachytherapy. A significant negative correlation between blood flow and time from therapy was present. CONCLUSIONS: Decreases in retinal and choroidal blood flow by laser speckle flowgraphy were detected within the first 6 months following brachytherapy. Reduced retinal and choroidal blood flow may be an early indicator of microangiographic response to radiation therapy.


Assuntos
Braquiterapia , Velocidade do Fluxo Sanguíneo/fisiologia , Corioide/irrigação sanguínea , Estudos Transversais , Humanos , Radioisótopos do Iodo , Fluxometria por Laser-Doppler , Lasers , Melanoma , Neoplasias Uveais
14.
PLoS One ; 17(6): e0270501, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35759478

RESUMO

PURPOSE: The current study aimed to investigate the time course of changes in choroidal circulation hemodynamics and their relationship to systemic circulation dynamics during the normal menstrual cycle in young, healthy women using laser speckle flowgraphy (LSFG). METHODS: This prospective study included 26 eyes from 13 young, healthy women (21.3 ± 4.0 years) with a normal menstrual cycle and 24 eyes from 12 young, healthy men (21.8 ± 4.4 years) as a control group. The macular mean blur rate (MBR), a quantitative index of relative blood flow velocity in the choroid, was measured using LSFG. MBR, intraocular pressure (IOP), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean blood pressure (MBP), and ocular perfusion pressure (OPP) were evaluated in the late follicular phase and mid-luteal phase in women and at baseline and 10 days after baseline in men, respectively. RESULTS: In the female group, IOP, SBP, DBP, MBP, and OPP values were significantly higher in the mid-luteal phase than those observed in the late follicular phase (P = 0.035, P < 0.001, P = 0.041, P = 0.001, P = 0.014, respectively). The average macular MBR values in the late follicular phase and mid-luteal phase were 12.7 ± 5.3 and 13.7 ± 6.6 (+7.7 ± 19.4%), representing a significant increase in the mid-luteal phase (P = 0.041). The rate of change in MBR exhibited a significant positive correlation with changes in DBP and MBP (R = 0.456, P = 0.019 and R = 0.474, P = 0.014). However, there were no significant changes in any of the factors in the male group during the study period. CONCLUSIONS: Our results suggest that in young, healthy women with a normal menstrual cycle, choroidal blood flow velocity decreases during the late follicular phase and increases during the mid-luteal phase, depending on systemic circulatory dynamics.


Assuntos
Corioide , Hemodinâmica , Velocidade do Fluxo Sanguíneo/fisiologia , Corioide/irrigação sanguínea , Feminino , Humanos , Fluxometria por Laser-Doppler/métodos , Masculino , Ciclo Menstrual , Estudos Prospectivos , Fluxo Sanguíneo Regional/fisiologia
16.
Zhonghua Shao Shang Za Zhi ; 38(5): 481-485, 2022 May 20.
Artigo em Chinês | MEDLINE | ID: mdl-35599424

RESUMO

The accurate diagnosis of burn wound depth is particularly important for evaluating the disease prognosis of burn patients. In the past, the diagnosis of burn wound depth often relied on the subjective judgment of doctors. With the continuous development of diagnostic technology, the methods for judging the depth of burn wound have also been updated. This paper mainly summarizes the research progress in the applications of indocyanine green angiography, laser Doppler imaging, laser speckle contrast imaging, and artificial intelligence in the diagnosis of burn wound depth, and compares the advantages and disadvantages of these techniques, so as to provide ideas for accurate diagnosis of burn wound depth.


Assuntos
Queimaduras , Cicatrização , Angiografia , Inteligência Artificial , Queimaduras/diagnóstico , Humanos , Fluxometria por Laser-Doppler/métodos , Pele
17.
Microvasc Res ; 142: 104373, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35513175

RESUMO

BACKGROUND: Metabolic syndrome (MetS) is associated with high cardiovascular morbidity and mortality, and endothelial dysfunction is an early pathogenetic event in the MetS. Lifestyle changes and pharmacological intervention might partly restore endothelial function in MetS. Whereas an optimal non-invasive test for endothelial dysfunction is still being sought, the aim of this study was to assess the relationship between changes in skin microvascular endothelial function, detected by Laser Doppler flowmetry, and cardiovascular risk factors (CVRFs) of patients with MetS. DESIGN AND METHODS: 3081 patients (1865 women and 1216 men, mean age 53 ± 6 years) with MetS were enrolled in the study, which was conducted during the period of 2010-2014 at Vilnius University Hospital Santaros Klinikos. Skin microvascular endothelial function was evaluated using the Laser Doppler flowmetry in combination with the post-occlusive reactive hyperaemia test. The percentage change of flow from peak to the rest flow (PF-RF) was calculated and used as the main measure of endothelial function. RESULTS: The study showed that decrease in flow-mediated dilatation reflected by PF-RF was associated with increased triglycerides (p = 0.002), male sex (p < 0.001), and diabetes (p = 0.002). Patients with quite a few CVRFs (body mass index ≥25 kg/m2, smoking, diabetes, arterial hypertension, a positive history of dyslipidaemia) had significantly lower PF-RF score than patients only with one of these risk factors (p < 0.001). CONCLUSIONS: Changes in skin microvascular endothelial function are significantly associated with most CVRFs and depend on the number of CVRFs.


Assuntos
Diabetes Mellitus , Hipertensão , Síndrome Metabólica , Feminino , Humanos , Fluxometria por Laser-Doppler , Masculino , Síndrome Metabólica/complicações , Síndrome Metabólica/diagnóstico , Microcirculação , Pessoa de Meia-Idade , Pele/irrigação sanguínea
18.
Anesth Prog ; 69(1): 31-38, 2022 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-35377931

RESUMO

OBJECTIVE: An ideal local anesthetic would be effective, minimally reduce pulpal blood flow (PBF), and not require injection. This study compared the effects of 3% tetracaine plus 0.05% oxymetazoline nasal spray (Kovanaze; KNS) and injections using 2% lidocaine with 1:100,000 epinephrine (LE) or 3% mepivacaine plain (MP) on PBF, anesthetic efficacy, and participant preference. METHODS: In a double-blind cross-over design, 20 subjects randomly received a test anesthetic and placebo at each of 3 visits (KNS/mock infiltration; mock nasal spray/LE; or mock nasal spray/MP). Nasal sprays and infiltration apical to a maxillary central incisor were delivered ipsilaterally. PBF was evaluated by laser Doppler flowmetry, and local anesthetic success was assessed with electric pulp testing. Postoperative pain levels, participant preference, and adverse events were also assessed. RESULTS: LE injections demonstrated significant reductions in PBF at all time intervals compared with baseline (P < .05), whereas KNS and MP did not. Pulpal anesthesia success rates were higher for LE (85%) compared with MP (35%) and KNS (5%). Participants reported significantly higher postoperative pain levels for KNS compared with LE and MP. Additionally, KNS was the least preferred of the anesthetics administered and resulted in more reported adverse events. CONCLUSION: Although KNS showed no significant effect on PBF, it was not effective in achieving pulpal anesthesia as used in this study.


Assuntos
Oximetazolina , Tetracaína , Anestésicos Locais , Humanos , Fluxometria por Laser-Doppler , Maxila
19.
Scand J Clin Lab Invest ; 82(3): 238-245, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35404718

RESUMO

This study aims to compare skin perfusion pressure measurements (SPP) at midfoot and below knee level performed with a novel laser Doppler flowmetry (LD) probe with a reference method based on photo plethysmography (PP). It includes 40 patients referred with known or suspected peripheral arterial disease. The SPP was performed with both devices. Blinded re-interpretation of the SPP measurements was carried out by three observers and a consensus quality score was provided for each measurement. SPP >40 mmHg was considered a clinically relevant cut-off. This study evaluates a total of 48 paired measurements of the midfoot and 54 below knee. The two methods agreed in overall diagnostic classification in 80 of 102 measurements (78%) with both methods showing SPP ≥40 mmHg in 21 cases, and both methods showing SPP <40 mmHg in 59 cases. Of the 22 participants with disagreement (22%) in overall classification, 15 had measurements within the range of 30-50 mmHg, and 7 with a clinically relevant disagreement with one device showing SPP <30 mmHg and the other ≥40 mmHg. Analysis of inter observer variation for the LD readings showed an intraclass correlation coefficient of 0.880 (95% CI: 0.807 to 0.929, p- value <0.05) at midfoot, and 0.933 (95% CI: 0.894 to 0.959, p-value <0.05) at below knee level. The novel probe based on LD showed good correlation with PP in absolute pressures, sufficient agreement in overall disease classification as well as good to excellent reliability in terms of inter observer variation.


Assuntos
Doença Arterial Periférica , Humanos , Fluxometria por Laser-Doppler/métodos , Perfusão , Doença Arterial Periférica/diagnóstico , Pletismografia , Reprodutibilidade dos Testes
20.
PLoS One ; 17(4): e0266684, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35421147

RESUMO

PURPOSE: We examined the effects of aging and exercise habits on the ocular blood flow (OBF) and its profiles throughout the optic nerve head region and choroidal area. We hypothesized that exercise habits reduce the stiffness of vessels in the ocular circulation, which generally increases with aging. METHODS: Participants in a medical checkup program (698 males and 192 females aged 28 to 80 years) were categorized into 2 groups (with and without exercise habits) based on participant self-reporting and the definition of the Ministry of Health, Labor and Welfare of Japan (MHLW). OBF in the right eye was measured and analyzed using laser speckle flowgraphy. The blowout time (BOT), which is the time during which the blood flow is higher than half of the mean of the minimum and maximum signals during one heartbeat, was calculated as an index of the blood flow profile. BOT has been used as an indicator of the flexibility of blood vessels. RESULTS: BOT significantly decreased with aging. Neither the self-reported nor MHLW-based exercise habits significantly affected the ocular circulation. CONCLUSION: These results indicate that the stiffness of the ocular vessels increases with aging, and this cannot be prevented by exercise habits.


Assuntos
Envelhecimento , Disco Óptico , Envelhecimento/fisiologia , Velocidade do Fluxo Sanguíneo/fisiologia , Feminino , Hábitos , Humanos , Fluxometria por Laser-Doppler/métodos , Masculino , Disco Óptico/irrigação sanguínea , Fluxo Sanguíneo Regional/fisiologia
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