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1.
Med Klin Intensivmed Notfmed ; 115(1): 43-51, 2020 Feb.
Artigo em Alemão | MEDLINE | ID: mdl-30397762

RESUMO

BACKGROUND: According to ILCOR (International Liaison Committee on Resuscitation) recommendations (released in 2003), use of therapeutic hypothermia is recommended for unconscious adult patients who have survived a cardiac arrest regardless of the initial monitored cardiac rhythm. Thereby, the treatment goal is to achieve and maintain a body temperature of 32-34 °C for a period of 12-24 h. According to the October 2015 recommendations of the European Resuscitation Council (ERC), targeted temperature management (TTM) remains part of treatment, but, as an option, it is advised that the targeted body temperature be 36 °C rather than 32-34 °C. PATIENT POPULATION AND METHODS: For a non-randomized retrospective observational study, a total of 149 patients were treated with cardiopulmonary resuscitation (CPR) between May 1999 and September 2009. For the first 4 days after CPR, data associated with demography, resuscitation, therapy (temperature course, neuron-specific enolase [NSE]) and clinical-neurological development (Glasgow Outcome Scale [GOS]) were collected. In the study, patients receiving mild hyperthermia were compared with those who did not receive hypothermia. RESULTS: Of the 149 patients included, 90 were treated with mild hypothermia (as decided by the attending physician), while 59 received no hypothermia therapy. Assessment reveals that mild hypothermia positively influences clinical-neurological progression, but not survival. On day three and four, patients with an unfavorable neurological progression exhibited significantly increased serum levels of NSE (day 4: 108.7 ± 137.3 ng/ml versus 25.5 ± 15.4 ng/ml). Patients receiving hypothermia showed lower average NSE levels compared with persons not receiving hypothermia. Furthermore, during the first 4 days, their NSE values tended to increase slower (NSE value at day 4: 55.9 ± 64.9 ng/ml versus 129.9 ± 174.9 ng/ml). The best cut-off-value for an unfavorable neurological result was 74.2 ng/ml at day four (specificity 100%, sensitivity 48.6%). For the group of patients who received hypothermia, the best cut-off-value was 74.2 ng/ml at day four (specificity 100%, sensitivity 40.9%), and, for the comparison group, best cut-off-value was 25.5 ng/ml at day three (specificity 100%, sensitivity 88.2%). CONCLUSION: After out-of-hospital resuscitation, there is a trend for improved clinical-neurological progression with mild hypothermia but it does not influence the prognostic significance of serum NSE. After assessment of available data, it is not possible to recommend uniform cut-off values for patients who received mild therapeutic hypothermia and for those who did not receive hypothermia treatment.


Assuntos
Reanimação Cardiopulmonar , Hipotermia Induzida , Parada Cardíaca Extra-Hospitalar , Fosfopiruvato Hidratase , Adulto , Humanos , Parada Cardíaca Extra-Hospitalar/diagnóstico , Parada Cardíaca Extra-Hospitalar/terapia , Fosfopiruvato Hidratase/análise , Prognóstico , Estudos Retrospectivos
2.
Zh Nevrol Psikhiatr Im S S Korsakova ; 119(8. Vyp. 2): 53-62, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31825363

RESUMO

The article presents a review of the literature on neuron-specific enolase (NSE) as a biomarker of stroke. It is shown that NSE does not allow differentiation of the ischemic and hemorrhagic process in stroke, but is suitable for determining the extent of brain tissue destruction both in the first hours of stroke and in the dynamics. The HSE analysis can be useful for monitoring the course of the disease, control of the dynamics of the pathological process, including when the size of the lesion increases, for evaluating the effectiveness of therapy and as a prognostic biomarker.


Assuntos
Isquemia Encefálica , Fosfopiruvato Hidratase , Acidente Vascular Cerebral , Biomarcadores/análise , Encéfalo , Humanos , Fosfopiruvato Hidratase/análise , Acidente Vascular Cerebral/diagnóstico
3.
Biosens Bioelectron ; 141: 111416, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31279179

RESUMO

Lung cancer is primary cancer threatening human life worldwide with the highest mortality rate. The early detection of lung cancer plays a critical role in the early diagnosis and subsequent treatment. However, the conventional methodologies limit the applications due to the low sensitivity, being expensive, and invasive procedure. Tumor markers as biochemical parameters can reflect cancer occurrence and progression, which show sensitivity, convenience, and low cost in developing biosensors, and act as good candidates for fabricating biosensors of detecting lung cancer. This review describes various biosensors (2013-2019) for detection of lung cancer biomarkers. Firstly, the various reported tumor markers of lung cancer are briefly described. Then, the advancements of designing biosensors for sensitive, stable, and selective identification of lung cancer biomarkers are systematically provided, with a specific focus on the main clinical biomarkers such as neuron-specific enolase (NSE), cytokeratin 19 fragment (CYFRA 21-1). Finally, the recent challenges and further opportunities for developing effective biosensors for early diagnosis of lung cancer are discussed.


Assuntos
Técnicas Biossensoriais/métodos , Neoplasias Pulmonares/diagnóstico , Animais , Antígenos de Neoplasias/análise , Biomarcadores Tumorais/análise , Técnicas Biossensoriais/instrumentação , Detecção Precoce de Câncer/instrumentação , Detecção Precoce de Câncer/métodos , Desenho de Equipamento , Humanos , Queratina-19/análise , Fosfopiruvato Hidratase/análise
4.
Ann Ital Chir ; 90: 174-181, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31182701

RESUMO

INTRODUCTION: Due to irreversible damage following head trauma, many overlapping pathophysiological events occur including excitotoxicity, acidotoxicity, ionic imbalance, edema, oxidative stress inflammation and apoptosis. MATERIAL AND METHODS: In this this study, after the rats were separated in to groups theserats were fed throughout fourteen days with betaine, omega-3 or betaine+omega-3 combination in physiological limits prior to the trauma. After a closed head trauma, the damaged brain tissues were collected for biochemically and histologically analyses. This examination involved analyses of levels of caspase-3 and cytochrome C and neuron-specific enolase (NSE) levels in brain tissue. RESULTS: These analyses showed that traumatic brain injury (TBI) caused an increase in the levels of caspase-3, cytochrome C and neuron-specific enolase (NED) in the brain tissues examined. DISCUSSION: In this study, apoptotic and/or necrotic cell death via mitochondrial cytochrome C caspase pathway in traumatized cells and neuron-specific enolase (NED) increase indicative of neuronal damage confirmed the research hypothesis. CONCLUSION: Level of the biomarkers induced by brain injury in the groups fed with betaine, omega-3 and betaine+omega-3 combination before the traumatic damage approximated to that of control group values, suggesting that these products may have a neuroprotective role. KEY WORDS: Betain, Caspase-3, Cytochrome C and Neuron-specific enolase, Omega-3, Traumatic brain injury.


Assuntos
Betaína/administração & dosagem , Lesões Encefálicas Traumáticas/prevenção & controle , Suplementos Nutricionais , Ácidos Graxos Ômega-3/administração & dosagem , Animais , Biomarcadores/análise , Química Encefálica , Caspase 3/análise , Grupo dos Citocromos c/análise , Fosfopiruvato Hidratase/análise , Ratos
5.
Biosens Bioelectron ; 141: 111331, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31233985

RESUMO

This study describes the construction of highly-sensitive photo-electrochemical (PEC) immunosensor for the detection of neuron-specific enolase (NSE). The biosensing platform is comprised of photo-active NiWO4 nanostructures, in-situ-grown over a conductive substrate (indium tin oxide) using a low-temperature template-based co-precipitation approach. The discussed approach enables the formation of discrete, yet morphologically-analogous, nanostructures with complete coverage (pinhole-free) of the electrode surface. The in-situ-grown nanostructure possess dense population with sharp saw-blade like morphological features that can support substantial immobilisation of anti-NSE agent. The constructed platform demonstrated excellent photo-catalytic activity towards uric acid (UA) which served as the base for the Electrochemical -mechanism (EC) based PEC inhibition sensing. The detection of NSE, relied on its obstruction in analytical signal observed for the photo-oxidation of UA after binding to the electrode surface via protein-antibody interaction. The constructed PEC immunosensor exhibits signal sensitivity up to 0.12 ng mL-1 of NSE with excellent signal reproducibility and electrode replicability. Moreover, the constructed platform was successfully used for NSE determination in human serum samples.


Assuntos
Técnicas Biossensoriais/métodos , Nanoestruturas/química , Níquel/química , Óxidos/química , Fosfopiruvato Hidratase/sangue , Tungstênio/química , Técnicas Eletroquímicas/métodos , Eletrodos , Humanos , Imunoensaio/métodos , Luz , Nanoestruturas/ultraestrutura , Nanotecnologia/métodos , Fosfopiruvato Hidratase/análise , Compostos de Estanho/química
6.
Vet Immunol Immunopathol ; 212: 23-26, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31213248

RESUMO

Renal α-enolase has variable expression in inflammatory and neoplastic diseases. Therefore, in order to define the distribution of α-enolase in renal tissues of cats, an immunohistochemistry assay was validated and described here. Tissues from 29 cats with IRIS Stage 2-4 CKD, 8 control cats < 2 years of age, and 4 control cats> 10 years of age were assessed. Interstitial nephritis was the predominant histopathological finding in the CKD group. The control cats < 2 years of age had moderate α-enolase immunoreactivity in tubular epithelium but staining was absent to mild in glomeruli. In contrast, α-enolase was moderate to high in tubular epithelium and glomeruli in control cats > 10 years of age. In cats with CKD, α-enolase was decreased in tubules that were degenerative or atrophic, similar to normal tubules in control groups, and moderate to high in glomeruli. When compared between the study groups, the results suggest that alpha-enolase decreases in damaged tubules and increases in the glomeruli of older cats prior to the development of detectable CKD. Further studies will be required to determine whether these findings relate to the pathogenesis or could be used in the diagnosis of feline CKD.


Assuntos
Rim/enzimologia , Rim/patologia , Fosfopiruvato Hidratase/análise , Insuficiência Renal Crônica/enzimologia , Insuficiência Renal Crônica/patologia , Animais , Doenças do Gato/enzimologia , Doenças do Gato/patologia , Gatos , Imuno-Histoquímica , Coloração e Rotulagem
8.
Biosens Bioelectron ; 136: 84-90, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31039491

RESUMO

Simultaneous detection of multiple tumor biomarkers in body fluids could facilitate early diagnosis of lung cancer, so as to provide scientific reference for clinical treatment. This paper depicted a multi-parameter paper-based electrochemical aptasensor for simultaneous detection of carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) in a clinical sample with high sensitivity and specificity. The paper-based device was fabricated through wax printing and screen-printing, which enabled functions of sample filtration and sample auto injection. Amino functional graphene (NG)-Thionin (THI)- gold nanoparticles (AuNPs) and Prussian blue (PB)- poly (3,4- ethylenedioxythiophene) (PEDOT)- AuNPs nanocomposites were synthesized respectively. They were used to modify the working electrodes not only for promoting the electron transfer rate, but also for immobilization of the CEA and NSE aptamers. A label-free electrochemical method was adopted, enabling a rapid simple point-of-care testing. Experimental results showed that the proposed multi-parameter aptasensor exhibited good linearity in ranges of 0.01-500 ng mL-1 for CEA (R2 = 0.989) and 0.05-500 ng mL-1 for NSE (R2 = 0.944), respectively. The limit of detection (LOD) was 2 pg mL-1 for CEA and 10 pg mL-1 for NSE. In addition, the device was evaluated using clinical serum samples and received a good correlation with large electrochemical luminescence (ECL) equipment, which would offer a new platform for early cancer diagnostics, especially in those resource-limit areas.


Assuntos
Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Microfluídica/métodos , Papel , Antígeno Carcinoembrionário/análise , Eletrodos , Ouro/química , Humanos , Neoplasias Pulmonares/diagnóstico , Nanopartículas Metálicas/química , Fosfopiruvato Hidratase/análise
9.
Crit Care ; 23(1): 171, 2019 05 14.
Artigo em Inglês | MEDLINE | ID: mdl-31088512

RESUMO

BACKGROUND: Cerebral hypoperfusion may aggravate neurological damage after cardiac arrest. Near-infrared spectroscopy (NIRS) provides information on cerebral oxygenation but its relevance during post-resuscitation care is undefined. We investigated whether cerebral oxygen saturation (rSO2) measured with NIRS correlates with the serum concentration of neuron-specific enolase (NSE), a marker of neurological injury, and with clinical outcome in out-of-hospital cardiac arrest (OHCA) patients. METHODS: We performed a post hoc analysis of a randomised clinical trial (COMACARE, NCT02698917) comparing two different levels of carbon dioxide, oxygen and arterial pressure after resuscitation from OHCA with ventricular fibrillation as the initial rhythm. We measured rSO2 in 118 OHCA patients with NIRS during the first 36 h of intensive care. We determined the NSE concentrations from serum samples at 48 h after cardiac arrest and assessed neurological outcome with the Cerebral Performance Category (CPC) scale at 6 months. We evaluated the association between rSO2 and serum NSE concentrations and the association between rSO2 and good (CPC 1-2) and poor (CPC 3-5) neurological outcome. RESULTS: The median (inter-quartile range (IQR)) NSE concentration at 48 h was 17.5 (13.4-25.0) µg/l in patients with good neurological outcome and 35.2 (22.6-95.8) µg/l in those with poor outcome, p < 0.001. We found no significant correlation between median rSO2 and NSE at 48 h, rs = - 0.08, p = 0.392. The median (IQR) rSO2 during the first 36 h of intensive care was 70.0% (63.5-77.0%) in patients with good outcome and 71.8% (63.3-74.0%) in patients with poor outcome, p = 0.943. There was no significant association between rSO2 over time and neurological outcome. In a binary logistic regression model, rSO2 was not a statistically significant predictor of good neurological outcome (odds ratio 0.99, 95% confidence interval 0.94-1.04, p = 0.635). CONCLUSIONS: We found no association between cerebral oxygenation measured with NIRS and NSE concentrations or outcome in patients resuscitated from OHCA. TRIAL REGISTRATION: ClinicalTrials.gov, NCT02698917 . Registered on 26 January 2016.


Assuntos
Cérebro/irrigação sanguínea , Parada Cardíaca Extra-Hospitalar/complicações , Perfusão/normas , Fosfopiruvato Hidratase/análise , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Adulto , Idoso , Pressão Arterial/fisiologia , Biomarcadores/análise , Dióxido de Carbono/análise , Cérebro/fisiopatologia , Distribuição de Qui-Quadrado , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Parada Cardíaca Extra-Hospitalar/sangue , Parada Cardíaca Extra-Hospitalar/fisiopatologia , Oxigênio/análise , Prognóstico , Estudos Prospectivos , Estatísticas não Paramétricas , Análise de Sobrevida , Fibrilação Ventricular/sangue , Fibrilação Ventricular/complicações , Fibrilação Ventricular/fisiopatologia
10.
Forensic Sci Int ; 298: 161-168, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30909103

RESUMO

We report the preliminary observations of the peptide content of decomposition fluid produced under controlled laboratory conditions and in the absence of a soil matrix. Four domestic pig (Sus scrofa domesticus) cadavers were used to model human decomposition over a four-week trial period; physical characteristics were recorded and the peptide components of decomposition fluid was analysed using high performance liquid chromatography-time of flight mass spectrometry. Preliminary data analysis indicated that a range of peptides were consistently detected across the course of the trial period and 27 of these were common to all four cadavers; 22 originating from haemoglobin. The peptides associated with haemoglobin subunit alpha and beta displayed a breakdown pattern that remained consistent for all cadavers for the duration of the trial. Though identification of peptides during decomposition has potential for estimating the time since death, quantification of selected peptides is likely to be essential to identify time-dependent trends.


Assuntos
Peptídeos/análise , Mudanças Depois da Morte , Animais , Biomarcadores/análise , Creatina Quinase/análise , Patologia Legal , Subunidades de Hemoglobina , Humanos , Modelos Animais , Fosfopiruvato Hidratase/análise , Proteólise , Piruvato Quinase/análise , Suínos
11.
Transl Res ; 201: 26-39, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30179587

RESUMO

Patients with systemic lupus erythematosus frequently develop lupus nephritis (LN), a condition that can lead to end-stage kidney disease. Multiple serum and urine biomarkers for LN have been proposed in recent years, yet none have become incorporated into clinical use. The majority of studies have been single center with significant variability in cohorts, assays, and sample storage, leading to inconclusive results. It has become clear that no single biomarker is likely to be sufficient to diagnose LN, identify flares, and define the response to therapy and prognosis. A more likely scenario is a panel of urine, serum, tissue, and genetic biomarkers. In this review, we summarize traditional and novel biomarkers and discuss how they may be utilized in order to bring precision medicine to clinical practice in LN.


Assuntos
Biomarcadores/análise , Nefrite Lúpica/diagnóstico , Medicina de Precisão , Anexinas/análise , Via Alternativa do Complemento , Via Clássica do Complemento , Humanos , MicroRNAs/urina , Fosfopiruvato Hidratase/análise
12.
Medicine (Baltimore) ; 97(31): e11675, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30075558

RESUMO

It remains controversial whether the distal rectal pouch should be either resected or used for reconstruction in anorectoplasty for the treatment of anorectal malformations (ARMs). Hence the aim of this study was to investigate whether ARMs were associated with a global neuromuscular maldevelopment of the terminal rectum specimens.There were 36 cases of ARMs (25 recto-bulbar fistula and 11 recto-prostatic fistula) and 10 healthy controls. The hematoxylin and eosin and Masson trichrome stain were used to conduct the histologic examination. The immunohistochemistry (IHC) and Western blot were conducted to analyze the neuron-specific enolase (NSE), S-100 protein, interstitial cells of Cajal marker (C-kit) within the rectal specimens in control group and ARM group.The most frequently observed histologic findings in mucosa were inflammation, congestion, eroded, and hemorrhage in the ARM cases. Submucosal inflammation and congestion were the most common submucosal findings in the ARM cases. Disrupted muscularis propria was observed in 60% of ARM cases. Mature ganglionic cells were reduced and muscularis propria showed reduced and patchy positivity for NSE, S-100, and C-kit protein in ARM group compared to that in control group according to IHC. Western blotting showed the expression levels of NSE, S-100, and C-kit were lower in the ARM group than that in the control group (P < .01).Histopathologic and IHC findings suggest that the distal rectal pouch has distinct defects in the neuromusculature. So it suggested that ARMs are abnormally developed tissue and need to be resected for better functional outcomes of the remaining gut.


Assuntos
Malformações Anorretais/patologia , Mucosa Intestinal/patologia , Reto/patologia , Biomarcadores/análise , Feminino , Humanos , Imuno-Histoquímica , Lactente , Masculino , Fosfopiruvato Hidratase/análise , Estudos Retrospectivos , Proteínas S100/análise , Telócitos/patologia
13.
Crit Care ; 22(1): 150, 2018 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-29871657

RESUMO

Hypoxic-ischaemic brain injury (HIBI) is the main cause of death in patients who are comatose after resuscitation from cardiac arrest. A poor neurological outcome-defined as death from neurological cause, persistent vegetative state, or severe neurological disability-can be predicted in these patients by assessing the severity of HIBI. The most commonly used indicators of severe HIBI include bilateral absence of corneal and pupillary reflexes, bilateral absence of N2O waves of short-latency somatosensory evoked potentials, high blood concentrations of neuron specific enolase, unfavourable patterns on electroencephalogram, and signs of diffuse HIBI on computed tomography or magnetic resonance imaging of the brain. Current guidelines recommend performing prognostication no earlier than 72 h after return of spontaneous circulation in all comatose patients with an absent or extensor motor response to pain, after having excluded confounders such as residual sedation that may interfere with clinical examination. A multimodal approach combining multiple prognostication tests is recommended so that the risk of a falsely pessimistic prediction is minimised.


Assuntos
Parada Cardíaca/complicações , Prognóstico , Biomarcadores/análise , Biomarcadores/sangue , Eletroencefalografia/métodos , Escala de Resultado de Glasgow , Parada Cardíaca/mortalidade , Humanos , Hipotermia Induzida/métodos , Hipóxia Encefálica/complicações , Hipóxia Encefálica/diagnóstico , Imagem por Ressonância Magnética/métodos , Exame Neurológico/métodos , Fosfopiruvato Hidratase/análise , Fosfopiruvato Hidratase/sangue , Qualidade de Vida , Subunidade beta da Proteína Ligante de Cálcio S100/análise , Subunidade beta da Proteína Ligante de Cálcio S100/sangue , Tomografia Computadorizada por Raios X/métodos
14.
Pathol Res Pract ; 214(6): 848-856, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29728311

RESUMO

Neuroendocrine differentiation (NED) is a common phenomenon in prostate cancer, and it has been associated with poor prognosis in some studies of primary prostate cancer. Incidence and patterns of NED in metastatic prostate cancer sites have not been examined widely. In this study, we studied expression of three commonly used markers of NED (chromogranin A, neuron specific enolase and synaptophysin) in 89 metastases from 31 men that died of castration-resistant prostate cancer and underwent rapid autopsy, and in 89 hormone-naïve primary tumors removed by radical prostatectomy. In addition, we examined NED association with androgen receptor, ERG and Ki-67 expression in metastatic tumor sites. Morphologically, 1 of 31 cases was classified as small cell carcinoma, and the remaining 30 were classified as usual prostate adenocarcinoma using a recently proposed classification of prostate cancers with NED. Metastases showed more expression of neuron specific enolase and synaptophysin compared to prostatectomies (6.3% of cells vs. 1.0%, p < 0.001 and 4.0% vs. 0.4%, p < 0.001, respectively). At least focal expression of one of the markers was seen in 78% of metastases. Strong expression was relatively uncommon, seen in 3/89 (chromogranin A), 8/89 (neuron specific enolase), and 5/89 (synaptophysin) metastases. Expression of chromogranin A and synaptophysin correlated with each other (r = 0.64, p < 0.001), but expression of neuron specific enolase did not correlate with the two other markers. Extent of NED varied significantly between different metastatic sites in individual patients. Absent androgen receptor expression was associated with strong expression of chromogranin A (p = .02) and neuron specific enolase (p = .02), but not with focal expression of any marker. No clear association was found between expression of NE markers and ERG or Ki-67. In conclusion, NED is a common and heterogeneous phenomenon in metastatic, castration-resistant prostate cancer. NED is more often present in castration-resistant prostate cancer compared to hormone-naïve disease, and it is associated with androgen receptor negativity. More research is needed to understand significance of NED in the progression of prostate cancer.


Assuntos
Antígenos de Diferenciação/análise , Biomarcadores Tumorais/análise , Metástase Neoplásica/patologia , Células Neuroendócrinas/patologia , Neoplasias de Próstata Resistentes à Castração/patologia , Adenocarcinoma/patologia , Carcinoma de Células Pequenas/patologia , Cromogranina A/análise , Cromogranina A/biossíntese , Humanos , Masculino , Fosfopiruvato Hidratase/análise , Fosfopiruvato Hidratase/biossíntese , Sinaptofisina/análise , Sinaptofisina/biossíntese
15.
Eur Rev Med Pharmacol Sci ; 22(6): 1595-1601, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29630101

RESUMO

OBJECTIVE: This study intended to explore the efficacy of computed tomography (CT)-guided implantation of iodine-125 (125I) seeds in the treatment of refractory malignant tumors with cancer pain and its influence on tumor markers in the serum. PATIENTS AND METHODS: 76 patients with refractory malignant tumors accompanied by cancer pain that received treatments in LongHua Hospital Shanghai University of Traditional Chinese Medicine from September 2013 to August 2014 were selected. They were divided into control group and observation group using a random number table (38 patients in each group). Patients in the control group received simple chemotherapy, while those in the observation group undergone CT-guided implantation of 125I seeds in combination with chemotherapy. Recent efficacy and 1-3-year survival rate were compared between the two groups of patients. The degree of pain relief after treatment was also compared between the two groups of patients. Electrochemiluminescence method was used to detect the concentrations of carcinoembryonic antigen (CEA), sugar chain antigen 199 (CA 199), sugar chain antigen 125 (CA 125), neuron-specific enolase (NSE) and cytokeratin-19-fragment (CYFRA21-1) in the two groups of patients before treatment, and 3 days, 7 days and 30 days after treatment. RESULTS: Recent disease control rate of the patients in the observation group was higher than that of the patients in the control group (p<0.05). The 1-3-year survival rate after surgery in the observation group was significantly higher than that in the control group (p<0.05). The total efficiency of pain control in the observation group was significantly higher than that in the control group (p<0.05). The levels of tumor markers in the two groups of patients were significantly decreased after treatment, while the reduction in the observation group was more evident than that in the control group (p<0.05). CONCLUSIONS: Our results showed that CT-guided implantation of 125I seeds is effective for the treatment of patients with refractory malignant tumors accompanied by cancer pain. It can reduce the levels of tumor markers, improve the survival rate and prolong the survival time of the patients.


Assuntos
Dor do Câncer/patologia , Neoplasias/radioterapia , Compostos Radiofarmacêuticos/uso terapêutico , Adulto , Idoso , Antígenos de Neoplasias/análise , Antineoplásicos/uso terapêutico , Antígeno Carcinoembrionário/análise , Feminino , Humanos , Radioisótopos do Iodo/química , Queratina-19/análise , Masculino , Pessoa de Meia-Idade , Neoplasias/tratamento farmacológico , Neoplasias/mortalidade , Fosfopiruvato Hidratase/análise , Índice de Gravidade de Doença , Taxa de Sobrevida , Tomografia Computadorizada por Raios X , Resultado do Tratamento
16.
J Bras Pneumol ; 44(1): 18-23, 2018.
Artigo em Inglês, Português | MEDLINE | ID: mdl-29538538

RESUMO

OBJECTIVE: To investigate the diagnostic value of α-enolase (ENO1) and serum ENO1 autoantibody levels in lung cancer. METHODS: Immunohistochemistry staining and ELISA were performed to detect ENO1 expression in lung tissue and serum ENO1 autoantibody levels, respectively. RESULTS: The expression of ENO1 was higher in lung cancer tissues than in benign lung disease tissues (p < 0.001). The proportion of lung cancer samples expressing ENO1 was not significantly different among the various pathological classification groups. The proportion of samples expressing ENO1 was higher in lung cancer patients in stages I/II than in those in stages III/IV (χ2 = 5.445; p = 0.018). The expression of ENO1 in lung cancer tissues was not associated with age, gender, or smoking history. Serum ENO1 antibody levels were significantly higher in the lung cancer group than in the benign lung disease and control groups (p < 0.001). The differences among the pathological classification groups were not statistically significant. Serum ENO1 antibody levels were also in lung cancer patients in stages I/II than in those in stages III/IV (p < 0.01). Serum ENO1 antibody levels were not associated with age, gender, or smoking history in lung cancer patients. The ROC curve representing the diagnosis of lung cancer based on ENO1 antibody levels had an area under the curve of 0.806. CONCLUSIONS: Our results suggest that high levels of ENO1 are associated with the clinical stage of lung cancer and that ENO1 expression and its serum autoantibody levels show diagnostic value in lung cancer.


Assuntos
Autoanticorpos/sangue , Carcinoma/enzimologia , Carcinoma/patologia , Neoplasias Pulmonares/enzimologia , Neoplasias Pulmonares/patologia , Fosfopiruvato Hidratase/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/análise , Carcinoma/diagnóstico , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/diagnóstico , Masculino , Pessoa de Meia-Idade , Valores de Referência , Sensibilidade e Especificidade , Estatísticas não Paramétricas
17.
Anal Biochem ; 548: 53-59, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29486202

RESUMO

In this work, a label-free electrochemical immunosensor was constructed on the base of poly p-phenylenediamine (PPD) and GR nanocomposite (PPD-GR). Screen-printed electrodes modified with PPD-GR nanocomposite and applied to advance enzyme-free and label free electrochemical immunosensor for detection of protein biomarker neuron-specific enolase (NSE). It was found that the PPD-GR nanocomposite exhibits excellent electrocatalytic activity towards ascorbic acid (AA) oxidation as analytical signal based on EC' mechanism. Due to the excellent electrocatalytic activity of PPD-GR nanocomposite, determination of NSE antigen was based on its obstruction to the electrocatalytic oxidation of AA after binding to the surface of electrode through interaction with the anti-NSE. The proposed immunosensor exhibited a wide linear range of 1.0-1000 ng mL-1, with a low detection limit of 0.3 ng mL-1. Furthermore, the proposed immunosensor were successfully used for the determination of NSE antigen in human serum samples.


Assuntos
Técnicas Eletroquímicas/métodos , Grafite/química , Nanocompostos/química , Fenilenodiaminas/química , Fosfopiruvato Hidratase/análise , Ácido Ascórbico/química , Humanos , Imunoensaio/métodos , Oxirredução , Fosfopiruvato Hidratase/metabolismo
18.
Mem Inst Oswaldo Cruz ; 113(3): 178-184, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29412357

RESUMO

BACKGROUND: Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiome, but are also major opportunistic pathogens that are responsible for significant mortality, especially in the case of bacteraemia and other severe infections, such as intra-abdominal abscesses. Up to now, several virulence factors have been described that might explain the involvement of B. fragilis in these infections. The secretion of extracellular membrane vesicles (EMVs) has been proposed to play a role in pathogenesis and symbiosis in gram-negative bacteria, by releasing soluble proteins and other molecules. In B. fragilis, these vesicles are known to have haemagglutination and sialidosis activities, and also contain a capsular polysaccharide (PSA), although their involvement in virulence is still not clear. OBJECTIVE: The aim of this study was to identify proteins in the EMV of the 638R B. fragilis strain by mass spectrometry, and also to assess for the presence of Bfp60, a surface plasminogen (Plg) activator, previously shown in B. fragilis to be responsible for the conversion of inactive Plg to active plasmin, which can also bind to laminin-1. METHODS: B. fragilis was cultured in a minimum defined media and EMVs were obtained by differential centrifugation, ultracentrifugation, and filtration. The purified EMVs were observed by both transmission electron microscopy (TEM) and immunoelectron microscopy (IM). To identify EMV constituent proteins, EMVs were separated by 1D SDS-PAGE and proteomic analysis of proteins sized 35 kDa to approximately 65 kDa was performed using mass spectrometry (MALDI-TOF MS). FINDINGS: TEM micrographs proved the presence of spherical vesicles and IM confirmed the presence of Bfp60 protein on their surface. Mass spectrometry identified 23 proteins with high confidence. One of the proteins from the B. fragilis EMVs was identified as an enolase P46 with a possible lyase activity. MAIN CONCLUSIONS: Although the Bfp60 protein was not detected by proteomics, α-enolase P46 was found to be present in the EMVs of B. fragilis. The P46 protein has been previously described to be present in the outer membrane of B. fragilis as an iron-regulated protein.


Assuntos
Bacteroides fragilis/enzimologia , Vesículas Extracelulares/enzimologia , Fosfopiruvato Hidratase/análise , Bacteroides fragilis/ultraestrutura , Eletroforese em Gel de Poliacrilamida , Vesículas Extracelulares/ultraestrutura , Humanos , Laminina , Espectrometria de Massas , Microscopia Eletrônica de Transmissão , Microscopia Imunoeletrônica , Fosfopiruvato Hidratase/metabolismo , Plasminogênio
19.
Analyst ; 143(4): 858-864, 2018 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-29327757

RESUMO

A rapid and sensitive detection of a cancer marker, neuron specific enolase (NSE), is demonstrated by using a disposable silver plasmonic chip functionalized with a mussel-inspired polydopamine (PDA) coating. A plasmonic chip consisting of a diffraction grating coated with a silver thin film is used for the excitation of propagating surface plasmon resonance through a rear-side grating coupling method. Simple and quick bio-functionalization of the sensor surface is performed by PDA coating which requires 20 min for deposition, and allows direct attachment of the capture antibody without using any coupling agents. A fluorescence based sandwich immunoassay is used for the detection of NSE by utilizing surface plasmon enhanced fluorescence (SPF) spectroscopy. The developed biosensor scheme provides approximately linear sensor responses for the sample containing NSE with the concentration around the clinically important value (12 ng mL-1) in both buffer and diluted human serum (25 vol% to a buffer solution). The detection limit for NSE is 0.5 ng mL-1 (11 pM) and 1.4 ng mL-1 (30 pM) in a buffer solution and diluted human serum, respectively. The presented biosensor scheme requires a small amount of the sample down to 10 µL in human serum and a short incubation time (15 min) of the sample solution containing NSE, enabling less invasive and rapid detection of NSE. This is the first example of the sensitive sandwich immunoassay demonstrated by using a plasmonic chip for the measurement of the sample dissolved in a complex medium with a rear side coupling method, which progresses the universal use of the SPF biosensors with a disposable plasmonic chip.


Assuntos
Técnicas Biossensoriais , Imunoensaio , Indóis , Fosfopiruvato Hidratase/análise , Polímeros , Ressonância de Plasmônio de Superfície , Fluorescência , Humanos
20.
Anal Biochem ; 540-541: 1-8, 2018 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-29113785

RESUMO

The content of neuron specific enolase (NSE) in serum is considered to be an essential indicator of small cell lung cancer (SCLC). Here, a novel label-free electrochemical immunoassay for the detection of NSE based on the three dimensionally macroporous reduced graphene oxide/polyaniline (3DM rGO/PANI) film has been proposed. The 3DM rGO/PANI film was constructed by electrochemical co-deposition of GO and aniline into the interspaces of a sacrificial silica opal template modified Au slice. During the co-deposition, GO was successfully reduced by aniline and PANI could be deposited on the surfaces of rGO sheets. The ratio of rGO and PANI in the composite was also optimized to achieve the maximum electrochemical performance. The 3DM rGO/PANI composite provided larger specific surface area for the antibody immobilization, exhibited enhanced conductivity for electron transfer, and more important was that PANI acted as the electroactive probe for indicating the NSE concentration. Under the optimal conditions, a linear current response of PANI to NSE concentration was obtained over 0.5 pg mL-1-10.0 ng mL-1 with a detection limit of 0.1 pg mL-1. Moreover, the immunosensor showed excellent selectivity, good stability, satisfactory reproducibility and regeneration, and was employed to detect NSE in clinical serum specimens.


Assuntos
Compostos de Anilina/química , Técnicas Eletroquímicas , Ensaios Enzimáticos/métodos , Grafite/química , Imunoensaio , Neurônios/enzimologia , Fosfopiruvato Hidratase/análise , Anticorpos Imobilizados/química , Anticorpos Imobilizados/imunologia , Biomarcadores Tumorais/sangue , Condutividade Elétrica , Eletrodos , Humanos , Concentração de Íons de Hidrogênio , Limite de Detecção , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/enzimologia , Óxidos/química , Fosfopiruvato Hidratase/sangue , Fosfopiruvato Hidratase/metabolismo , Porosidade , Dióxido de Silício/química , Espectroscopia de Infravermelho com Transformada de Fourier
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