Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 405
Filtrar
1.
Food Chem ; 372: 131272, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34628121

RESUMO

Bioactive compounds from strawberries have been associated with multiple healthy benefits. The present study aimed to assess chemical characterization of a methanolic extract of the Romina strawberry variety in terms of antioxidant capacity, polyphenols profile and chemical elements content. Additionally, potential toxicity, the effect on amyloid-ß production and oxidative stress of the extract was in vivo evaluated in the experimental model Caenorhabditis elegans. Results revealed an important content in phenolic compounds (mainly ellagic acid and pelargonidin-3-glucoside) and minerals (K, Mg, P and Ca). The treatment with 100, 500 or 1000 µg/mL of strawberry extract did not show toxicity. On the contrary, the extract was able to delay amyloid ß-protein induced paralysis, reduced amyloid-ß aggregation and prevented oxidative stress. The potential molecular mechanisms present behind the observed results explored by RNAi technology revealed that DAF-16/FOXO and SKN-1/NRF2 signaling pathways were, at least partially, involved.


Assuntos
Doença de Alzheimer , Proteínas de Caenorhabditis elegans , Fragaria , Peptídeos beta-Amiloides/genética , Animais , Antioxidantes , Caenorhabditis elegans/genética , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/genética , Proteínas de Ligação a DNA/metabolismo , Fatores de Transcrição Forkhead/genética , Fatores de Transcrição Forkhead/metabolismo , Fragaria/genética , Fragaria/metabolismo , Metanol , Estresse Oxidativo , Extratos Vegetais , Fatores de Transcrição/metabolismo
2.
Int J Mol Sci ; 22(24)2021 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-34948297

RESUMO

Six strawberry genotypes were examined for fruit yield and size, important chemical traits (sugars, phenolics, anthocyanins, ascorbic acid, volatiles) and antioxidant properties (ferric reducing power). In addition, we determined the expression of genes and transcription factors (SAAT, FaNES1, FaFAD1, FaEGS2, FaEOBII and FaMYB10) controlling the main flavor and aroma traits, and finally evaluated the effect of the genotype and harvest time on the examined chemical and genetic factors, as well as their intercorrelations. The commercial varieties 'Fortuna', 'Victory', 'Calderon', 'Rociera', and two advanced selections Ber22/6 and Ber23/3 were cultivated under the same conditions at Berryplasma World Ltd. plantations (Varda, Ilia, Region of Western Greece). Strawberries were harvested at three different time points over the main harvest period in Greece, i.e., early March (T1), late March (T2) and late April (T3). 'Fortuna' exhibited the highest early and total yield, while 'Calderon', the highest average berry weight. General Linear Model repeated measures ANOVA demonstrated that the interaction of the genotype and harvest time was significant (p < 0.001) on all tested quality attributes and gene expression levels, showing that each genotype behaves differently throughout the harvest period. Exceptions were observed for: (a) the volatile anhydrides, fatty acids, aromatics and phenylpropanoids (all were greatly affected by the harvest time), and (b) lactones, furaneol and FaEGS2 that were affected only by the genotype. We observed significant intercorrelations among those factors, e.g., the positive correlation of FaFAD1 expression with decalactone and nerolidol, of SAAT with furaneol, trans-cinnamic acid and phenylpropanoids, and of FaEGS2 with decalactone and FaFAD1. Moreover, a strong positive correlation between SAAT and FaMYB10 and a moderate negative one between SAAT and glucose were also detected. Those correlations can be further investigated to reveal potential markers for strawberry breeding. Overall, our study contributes to a better understanding of strawberry physiology, which would facilitate breeding efforts for the development of new strawberry varieties with superior qualitative traits.


Assuntos
Aromatizantes/metabolismo , Fragaria/genética , Locos de Características Quantitativas/genética , Compostos Orgânicos Voláteis/metabolismo , Antioxidantes/metabolismo , Fragaria/metabolismo , Frutas/genética , Frutas/metabolismo , Genótipo , Glucose/genética , Glucose/metabolismo , Grécia , Odorantes , Sesquiterpenos/metabolismo , Paladar/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
3.
Int J Mol Sci ; 22(19)2021 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-34638834

RESUMO

Strawberry (Fragaria × ananassa) fruit ripening is regulated by a complex of cellular signal transduction networks, in which protein kinases are key components. Here, we report a relatively simple method for assaying protein kinase activity in vivo and specifically its application to study the kinase, FaMPK6, signaling in strawberry fruit. Green fluorescent protein (GFP)-tagged FaMPK6 was transiently expressed in strawberry fruit and after stimuli were applied to the fruit it was precipitated using an anti-GFP antibody. The precipitated kinase activity was measured in vitro using 32P-ATP and myelin basic protein (MBP) as substrates. We also report that FaMPK6 is not involved in the abscisic acid (ABA) signaling cascade, which is closely associated with FaMPK6 signaling in other plant species. However, methyl jasmonate (MeJA), low temperature, and high salt treatments were all found to activate FaMPK6. Transient manipulation of FaMPK6 expression was observed to cause significant changes in the expression patterns of 2749 genes, of which 264 were associated with MeJA signaling. The data also suggest a role for FaMPK6 in modulating cell wall metabolism during fruit ripening. Taken together, the presented method is powerful and its use will contribute to a profound exploration to the signaling mechanism of strawberry fruit ripening.


Assuntos
Fragaria/metabolismo , Frutas/crescimento & desenvolvimento , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Proteínas de Plantas/metabolismo , Transdução de Sinais , Fragaria/genética , Frutas/genética , Proteínas Quinases Ativadas por Mitógeno/genética , Proteínas de Plantas/genética
4.
Int J Mol Sci ; 22(18)2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34576171

RESUMO

Hydrogen sulfide (H2S) plays several physiological roles in plants. Despite the evidence, the role of H2S on cell wall disassembly and its implications on fleshy fruit firmness remains unknown. In this work, the effect of H2S treatment on the shelf-life, cell wall polymers and cell wall modifying-related gene expression of Chilean strawberry (Fragaria chiloensis) fruit was tested during postharvest storage. The treatment with H2S prolonged the shelf-life of fruit by an effect of optimal dose. Fruit treated with 0.2 mM H2S maintained significantly higher fruit firmness than non-treated fruit, reducing its decay and tripling its shelf-life. Additionally, H2S treatment delays pectin degradation throughout the storage period and significantly downregulated the expression of genes encoding for pectinases, such as polygalacturonase, pectate lyase, and expansin. This evidence suggests that H2S as a gasotransmitter prolongs the post-harvest shelf-life of the fruit and prevents its fast softening rate by a downregulation of the expression of key pectinase genes, which leads to a decreased pectin degradation.


Assuntos
Fragaria/metabolismo , Frutas/metabolismo , Pectinas/metabolismo , Parede Celular/metabolismo , Gasotransmissores/metabolismo , Regulação da Expressão Gênica de Plantas , Sulfeto de Hidrogênio/metabolismo , Polissacarídeo-Liases/metabolismo
5.
Nat Commun ; 12(1): 4941, 2021 08 16.
Artigo em Inglês | MEDLINE | ID: mdl-34400639

RESUMO

Plant small RNAs are important regulatory elements that fine-tune gene expression and maintain genome integrity by silencing transposons. Reproductive organs of monocots produce abundant phased, small interfering RNAs (phasiRNAs). The 21-nt reproductive phasiRNAs triggered by miR2118 are highly enriched in pre-meiotic anthers, and have been found in multiple eudicot species, in contrast with prior reports of monocot specificity. The 24-nt reproductive phasiRNAs are triggered by miR2275, and are highly enriched during meiosis in many angiosperms. Here, we report the widespread presence of the 21-nt reproductive phasiRNA pathway in eudicots including canonical and non-canonical microRNA (miRNA) triggers of this pathway. In eudicots, these 21-nt phasiRNAs are enriched in pre-meiotic stages, a spatiotemporal distribution consistent with that of monocots and suggesting a role in anther development. Although this pathway is apparently absent in well-studied eudicot families including the Brassicaceae, Solanaceae and Fabaceae, our work in eudicots supports an earlier singular finding in spruce, a gymnosperm, indicating that the pathway of 21-nt reproductive phasiRNAs emerged in seed plants and was lost in some lineages.


Assuntos
Magnoliopsida/metabolismo , Nucleotídeos/metabolismo , RNA de Plantas/genética , RNA Interferente Pequeno/metabolismo , Sementes/metabolismo , Fragaria/genética , Fragaria/metabolismo , Regulação da Expressão Gênica de Plantas , Meiose , MicroRNAs/genética , Filogenia , Picea/genética , Proteínas de Plantas/genética , RNA de Cadeia Dupla/metabolismo , Solanaceae/metabolismo , Transcriptoma
6.
Biochem Biophys Res Commun ; 569: 93-99, 2021 09 10.
Artigo em Inglês | MEDLINE | ID: mdl-34237433

RESUMO

Salinity is one of the major environmental stresses that limit crop growth and productivity. In this study, the FvMYB24 gene that encodes an R2R3-type MYB transcription factor was cloned and characterized. An expression analysis showed that FvMYB24 had a tissue- and stage-specific profile and was induced by salt treatment. Arabidopsis plants that overexpressed transgenic FvMYB24 exhibited a higher germination rate, fresh weight, chlorophyll content, and longer root length than the wild type (WT) under salt stress. The transgenic plants had higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) and the accumulation of proline, while these plants accumulated lower amounts of malondialdehyde (MDA) compared with the WT. Furthermore, our results also revealed that the overexpression of FvMYB24 up-regulated the expression of several stress-related genes (AtSOS1, AtSOS2, AtSOS3, AtSOD, AtPOD, AtCAT1, AtNHX1, and AtLEA3) in response to salt stress, thus, enhancing the tolerance of transgenic Arabidopsis. An analysis of the cis-acting elements in the SOS1, SOS2, and SOS3 promoters revealed MYB-binding sites. However, FvMYB24 could only bind to the SOS1 promoter to mediate salt tolerance but not to the SOS2 and SOS3 promoters. These findings suggest that FvMYB24 could potentially be used as a positive regulator in transgenic plant breeding to improve the tolerance of strawberry plants to salt.


Assuntos
Arabidopsis/genética , Fragaria/genética , Proteínas de Plantas/genética , Estresse Salino/genética , Tolerância ao Sal/genética , Fatores de Transcrição/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Catalase/metabolismo , Fragaria/metabolismo , Regulação da Expressão Gênica de Plantas , Peroxidase/metabolismo , Filogenia , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , /metabolismo , Trocadores de Sódio-Hidrogênio/genética , Trocadores de Sódio-Hidrogênio/metabolismo , Superóxido Dismutase/metabolismo , Fatores de Transcrição/classificação , Fatores de Transcrição/metabolismo
7.
Int J Mol Sci ; 22(11)2021 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-34200124

RESUMO

Identifying and characterizing cold responsive genes in Fragaria vesca associated with or responsible for low temperature tolerance is a vital part of strawberry cultivar development. In this study we have investigated the transcript levels of eight genes, two dehydrin genes, three putative ABA-regulated genes, two cold-inducible CBF genes and the alcohol dehydrogenase gene, extracted from leaf and crown tissues of three F. vesca genotypes that vary in cold tolerance. Transcript levels of the CBF/DREB1 transcription factor FvCBF1E exhibited stronger cold up-regulation in comparison to FvCBF1B.1 in all genotypes. Transcripts of FvADH were highly up-regulated in both crown and leaf tissues from all three genotypes. In the 'ALTA' genotype, FvADH transcripts were significantly higher in leaf than crown tissues and more than 10 to 20-fold greater than in the less cold-tolerant 'NCGR1363' and 'FDP817' genotypes. FvGEM, containing the conserved ABRE promoter element, transcript was found to be cold-regulated in crowns. Direct comparison of the kinetics of transcript and protein accumulation of dehydrins was scrutinized. In all genotypes and organs, the changes of XERO2 transcript levels generally preceded protein changes, while levels of COR47 protein accumulation preceded the increases in COR47 RNA in 'ALTA' crowns.


Assuntos
Adaptação Fisiológica , Temperatura Baixa , Fragaria/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Fragaria/genética , Fragaria/metabolismo , Genótipo
8.
Int J Mol Sci ; 22(12)2021 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-34208198

RESUMO

The role of auxin in the fruit-ripening process during the early developmental stages of commercial strawberry fruits (Fragaria x ananassa) has been previously described, with auxin production occurring in achenes and moving to the receptacle. Additionally, fruit softening is a consequence of the depolymerization and solubilization of cell wall components produced by the action of a group of proteins and enzymes. The aim of this study was to compare the effect of exogenous auxin treatment on the physiological properties of the cell wall-associated polysaccharide contents of strawberry fruits. We combined thermogravimetric (TG) analysis with analyses of the mRNA abundance, enzymatic activity, and physiological characteristics related to the cell wall. The samples did not show a change in fruit firmness at 48 h post-treatment; by contrast, we showed changes in the cell wall stability based on TG and differential thermogravimetric (DTG) analysis curves. Less degradation of the cell wall polymers was observed after auxin treatment at 48 h post-treatment. The results of our study indicate that auxin treatment delays the cell wall disassembly process in strawberries.


Assuntos
Biopolímeros/metabolismo , Parede Celular/metabolismo , Fragaria/metabolismo , Frutas/metabolismo , Ácidos Indolacéticos/farmacologia , Parede Celular/efeitos dos fármacos , Parede Celular/genética , Fragaria/efeitos dos fármacos , Fragaria/genética , Frutas/efeitos dos fármacos , Frutas/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Temperatura , Termogravimetria , Transcrição Genética/efeitos dos fármacos , Ácidos Tri-Iodobenzoicos/farmacologia
9.
Molecules ; 26(14)2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34299427

RESUMO

Strawberry is the most consumed berry fruit worldwide due to its unique aroma and flavor. Drying fruits to produce a powder represents one of the possible conservation methods to extend their shelf-life. The aim of the present study was to compare the influence of freezing and different drying methods on the volatile profile of strawberry using the HS-SPME/GC-MS method, in addition to analysis of strawberry jam volatiles. A total of 165 compounds were identified, accounting for 85.03-96.88% of the total volatile compositions. Results and PCA showed that freezing and each drying process affected the volatile profile in a different way, and the most remarkable representative differential volatiles were ethyl hexanoate, hexyl acetate, (E)-2-hexenyl acetate, mesifurane, (E)-nerolidol, γ-decalactone, 1-hexanol, and acetoin. Shade air-dried, frozen, freeze-dried, and oven-dried 45 °C samples retained more of the fruity and sweet aromas of strawberry, representing more than 68% of the total aroma intensity according to the literature. In contrast, the microwave-drying method showed drastic loss of fruity esters. Strawberry jams demonstrated complete destruction of esters and alcohols in most jams, while terpenes were significantly increased. These findings help better understand the aroma of strawberry and provide a guide for the effects of drying, freezing, and jam processing.


Assuntos
Manipulação de Alimentos/métodos , Fragaria/química , Compostos Orgânicos Voláteis/análise , Álcoois/análise , Dessecação/métodos , Ésteres/análise , Aromatizantes/análise , Fragaria/metabolismo , Liofilização/métodos , Congelamento , Frutas/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Odorantes/análise , Paladar
10.
BMC Plant Biol ; 21(1): 295, 2021 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-34174836

RESUMO

BACKGROUND: Drought is a common phenomenon worldwide. It is also one of the main abiotic factors that affect the growth and quality of strawberry. The dehydration-responsive element binding protein (DREB) members that belong to the APETALA2/ethylene-responsive element binding protein (AP2/EREBP) superfamily are unique transcription factors in plants that play important roles in the abiotic stress response. RESULTS: Here, a total of 119 AP2/EREBP genes were identified in Fragaria vesca, and the AP2/EREBP superfamily was divided into AP2, RAV, ERF, DREB, and soloist subfamilies, containing 18, 7, 61, 32, and one member(s), respectively. The DREB subfamily was further divided into six subgroups (A-1 to A-6) based on phylogenetic analysis. Gene structure, conserved motifs, chromosomal location, and synteny analysis were conducted to comprehensively investigate the characteristics of FvDREBs. Furthermore, transcriptome analysis revealed distinctive expression patterns among the FvDREB genes in strawberry plants exposed to drought stress. The expression of FvDREB6 of the A-2 subgroup was down-regulated in old leaves and up-regulated in young leaves in response to drought. Furthermore, qRT-PCR analysis found that FvDREB8 from the A-2 subgroup had the highest expression level under drought stress. Together, analyses with the expression pattern, phylogenetic relationship, motif, and promoter suggest that FvDREB18 may play a critical role in the regulation of FvDREB1 and FvDREB2 expression. CONCLUSIONS: Our findings provide new insights into the characteristics and potential functions of FvDREBs. These FvDREB genes should be further studied as they appear to be excellent candidates for drought tolerance improvement of strawberry.


Assuntos
Fragaria/genética , Regulação da Expressão Gênica de Plantas/genética , Genes de Plantas/genética , Proteínas de Plantas/genética , Fatores de Transcrição/genética , Transcriptoma , Sequência Conservada , Desidratação , Fragaria/metabolismo , Fragaria/fisiologia , Regulação da Expressão Gênica de Plantas/fisiologia , Genes de Plantas/fisiologia , Estudo de Associação Genômica Ampla , Proteínas de Plantas/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Transcrição/fisiologia
11.
PLoS One ; 16(5): e0251351, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33984039

RESUMO

Water movements through the fruit skin play critical roles in many disorders of strawberry (Fragaria × ananassa Duch.) such as water soaking, cracking and shriveling. The objective was to identify the mechanisms of fruit water loss (dry skin, transpiration) and water uptake (wet skin, osmosis). Fruits were held above dried silica gel or incubated in deionized water. Water movements were quantified gravimetrically. Transpiration and osmotic uptake increased linearly with time. Abrading the thin cuticle (0.62 g m-2) increased rates of transpiration 2.6-fold, the rates of osmotic uptake 7.9-fold. The osmotic potential of the expressed juice was nearly the same for green and for white fruit but decreased in red fruit stages. Fruit turgor was low throughout development, except for green fruit. There was no relationship between the rates of water movement and fruit osmotic potential. The skin permeance for transpiration and for osmotic uptake were both high (relative to other fruit species) but were two orders of magnitude greater for osmotic uptake than for transpiration. Incubating fruit in isotonic solutions of osmolytes of different sizes resulted in increases in fruit mass that depended on the osmolyte. The rate of osmotic uptake decreased asymptotically as molecular size of the osmolyte increased. When transpiration and osmotic uptake experiments were conducted sequentially on the same fruit, the rates of transpiration were higher for fruit previously incubated in water. Fluorescence microscopy revealed considerable microcracking in a fruit previously incubated in water. Our findings indicate that the high permeance for osmotic uptake is accounted for by an extremely thin cuticle and by viscous water flow through microcracks and along polar pathways.


Assuntos
Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Transpiração Vegetal/fisiologia , Transporte Biológico , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Osmose , Pressão Osmótica/fisiologia , Permeabilidade , Água/metabolismo
12.
Food Chem ; 358: 129913, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33933955

RESUMO

Ethylene seems to play a secondary role in non-climacteric strawberry ripening compared to abscisic acid. However, this does not exclude that ethylene can regulate some specific events related to the ripening process. Preliminary experiments of applications of ethylene or its inhibitor 1-MCP to strawberry fruits have reinforced this hypothesis. Here, we reveal some previously non-covered physiological effects of ethylene using an in vitro strawberry ripening system. Fruits of Fragaria chiloensis treated with ethephon at the large green developmental stage showed inhibition of anthocyanin biosynthesis and downregulation of essential anthocyanin biosynthesis genes during the ripening. At the same time, ethylene stimulated lignin biosynthesis and remarkably upregulated the expression of FcPOD27. Since contrasting results have been reported when ethylene was applied at late ripening developmental stages, our findings support the hypothesis of a temporal-specific ethylene role in the ripening of strawberry fruits.


Assuntos
Antocianinas/biossíntese , Etilenos/farmacologia , Fragaria/efeitos dos fármacos , Frutas/efeitos dos fármacos , Lignina/metabolismo , Compostos Organofosforados/farmacologia , Regulação para Baixo/efeitos dos fármacos , Qualidade dos Alimentos , Fragaria/genética , Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Frutas/genética , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Reguladores de Crescimento de Plantas/farmacologia
13.
Plant Physiol ; 185(3): 1059-1075, 2021 04 02.
Artigo em Inglês | MEDLINE | ID: mdl-33793929

RESUMO

Unlike ovary-derived botanical fruits, strawberry (Fragaria x ananassa) is an accessory fruit derived from the receptacle, the stem tip subtending floral organs. Although both botanical and accessory fruits initiate development in response to auxin and gibberellic acid (GA) released from seeds, the downstream auxin and GA signaling mechanisms underlying accessory fruit development are presently unknown. We characterized GA and auxin signaling mutants in wild strawberry (Fragaria vesca) during early stage fruit development. While mutations in FveRGA1 and FveARF8 both led to the development of larger fruit, only mutations in FveRGA1 caused parthenocarpic fruit formation, suggesting FveRGA1 is a key regulator of fruit set. FveRGA1 mediated fertilization-induced GA signaling during accessory fruit initiation by repressing the expression of cell division and expansion genes and showed direct protein-protein interaction with FveARF8. Further, fvearf8 mutant fruits exhibited an enhanced response to auxin or GA application, and the increased response to GA was due to increased expression of FveGID1c coding for a putative GA receptor. The work reveals a crosstalk mechanism between FveARF8 in auxin signaling and FveGID1c in GA signaling. Together, our work provides functional insights into hormone signaling in an accessory fruit, broadens our understanding of fruit initiation in different fruit types, and lays the groundwork for future improvement of strawberry fruit productivity and quality.


Assuntos
Fragaria/metabolismo , Giberelinas/metabolismo , Proteínas de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Transdução de Sinais/genética , Transdução de Sinais/fisiologia
14.
Genes (Basel) ; 12(3)2021 03 08.
Artigo em Inglês | MEDLINE | ID: mdl-33800118

RESUMO

Genomic imprinting has drawn increasing attention in plant biology in recent years. At present, hundreds of imprinted genes have been identified in various plants, and some of them have been reported to be evolutionarily conserved in plant species. In this research, 17 candidate genes in Fragaria vesca were obtained based on the homologous imprinted genes in Arabidopsis thaliana and other species. We further constructed reciprocal crosses of diploid strawberry (F. vesca) using the varieties 10-41 and 18-86 as the parents to investigate the conservation of these imprinted genes. Potentially informative single nucleotide polymorphisms (SNPs) were used as molecular markers of two parents obtained from candidate imprinted genes which have been cloned and sequenced. Meanwhile, we analyzed the SNP site variation ratios and parent-of-origin expression patterns of candidate imprinted genes at 10 days after pollination (DAP) endosperm and embryo for the hybrids of reciprocal cross, respectively. A total of five maternally expressed genes (MEGs), i.e., FvARI8, FvKHDP-2, FvDRIP2, FvBRO1, and FvLTP3, were identified in the endosperm, which did not show imprinting in the embryo. Finally, tissues expression analysis indicated that the five imprinted genes excluding FvDRIP2 mainly expressed in the endosperm. This is the first report on imprinted genes of Fragaria, and we provide a simple and rapid method based on homologous conservation to screen imprinted genes. The present study will provide a basis for further study of function and mechanism of genomic imprinting in F. vesca.


Assuntos
Endosperma , Fragaria , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Impressão Genômica , Proteínas de Plantas , Endosperma/genética , Endosperma/metabolismo , Fragaria/genética , Fragaria/metabolismo , Proteínas de Plantas/biossíntese , Proteínas de Plantas/genética
15.
Genes (Basel) ; 12(4)2021 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-33916467

RESUMO

Fragaria × ananassa Duch, which among the youngest fruit crops, comprises many popular cultivars that are famous for their favored color and aroma. The regulation roles of AP2/ERF (APETALA2/ethylene-responsive element-binding factor) transcription factors in fruit flavor and color regulation have been studied in several fruit crops. The AP2 family of strawberry, which was ignored in recent AP2/ERF identification studies, was explored in this study. A total of 64 FaAP2 (Fragaria × ananassa AP2) transcription factors belonging to the euAP2, euANT (AINTEGUMENTA), and baselANT groups were identified with canonical insertion motifs in two AP2 domains. The motif identification illustrated that motifs 1, 5, and 2 indicated a corresponding AP2 domain repeat 1 with a linker region, and motifs 6, 4, 3 indicated a corresponding AP2 domain repeat 2, all of which were highly conserved. By synteny analysis, FaAP2 paralogs were identified in each sub-genome, and FaAP2 gene duplication and loss explained the unequal AP2 loci of sub-genomes. The expression profile in three cultivars indicated that six FaAP2 paralogs-four WRI (WRINKLED) gene homologs and two AP2 gene homologs-were candidate regulators of red fruit color and/or special fruit aroma. All these finds provide a basis for further investigations into role of AP2 in fruit color and aroma and would be helpful in the targeted selection of strawberry fruit quality to improve breeding.


Assuntos
Fragaria/genética , Frutas/genética , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Odorantes/análise , Proteínas de Plantas/genética , Fator de Transcrição AP-2/genética , Evolução Molecular , Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Filogenia , Proteínas de Plantas/metabolismo , Fator de Transcrição AP-2/metabolismo
16.
Food Chem ; 354: 129502, 2021 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-33752118

RESUMO

The dissipation behaviors and exposure risks of individual and joint application of procymidone and carbendazim in greenhouse strawberries were studied. The initial concentrations were similar after individual or joint applications, while the dissipation half-lives and finial concentrations were significantly different. After joint application, the dissipation half-lives of procymidone and carbendazim were 12.9 and 16.0 days, respectively, which were about 1.8 times higher than those after individual application. Furthermore, the final residues under joint application condition were 1.8-3.5 times higher than those under individual application condition. The joint application decreased the dissipation rates of procymidone or carbendazim in strawberries, and increased the final residue concentrations. The dietary intake risks of procymidone and carbendazim (whether applied individually or jointly) were no higher than 0.12, which were acceptable for human health. This work would shed a light for the guidance of the joint application and risk assessment of the typical fungicides in strawberry.


Assuntos
Benzimidazóis/metabolismo , Compostos Bicíclicos com Pontes/metabolismo , Carbamatos/metabolismo , Fragaria/química , Benzimidazóis/análise , Compostos Bicíclicos com Pontes/análise , Carbamatos/análise , Cromatografia Líquida de Alta Pressão , Fragaria/metabolismo , Efeito Estufa , Meia-Vida , Humanos , Resíduos de Praguicidas/análise , Medição de Risco , Espectrometria de Massas por Ionização por Electrospray
17.
Food Chem ; 351: 129284, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-33640773

RESUMO

Coating damage destroys the integrity features critical for maintaining the modified atmosphere inside the fruit. In this study, we developed a self-healing edible coating that maintains its barrier properties for extending the shelf life of strawberries. The coating was fabricated via the layer-by-layer assembly of chitosan (CS) and sodium alginate (SA). (SA/CS)3 formed by three assembly cycles could completely heal the visibly damaged area by treating water. The mechanical properties and the water and oxygen rates of the healed coating were 97%, 63%, and 95%, respectively, of the intact coating. (SA/CS)3 coating effectively delayed strawberry deterioration. Moreover, the coating reduced the impact of coating damage on strawberries by restoring the coating barrier properties. The present findings have important implications for solving the reduction in freshness caused by coating damage.


Assuntos
Filmes Comestíveis , Conservação de Alimentos/métodos , Alginatos/química , Quitosana/química , Qualidade dos Alimentos , Fragaria/química , Fragaria/metabolismo , Frutas/química , Frutas/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Oxigênio/química , Polietilenotereftalatos/química , Resistência à Tração , Água/química
18.
Carbohydr Polym ; 258: 117688, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33593561

RESUMO

Since shelf life of perishable foods is short, a compelling challenge is to prolong the freshness of foods with a cost-effective strategy. A perishable fruit, the strawberry, is chosen as a model perishable food and an edible film coating is applied to it using carboxymethylated cellulose nanofibers (CM-CNFs) stabilized by cationic salts. A transparent and impermeable CM-CNF film is formed at the strawberry surface using a dip coating process. The formation of the film is dependent on the electrostatic interaction between anionic CM-CNF and salt cations. Physical properties of the film are characterized and the effectiveness of edible film coating on the freshness of perishable fruit is evaluated by the measurement of weight loss, CO2 release, firmness, total solid sugar and acidity. Cellulose nanofiber is a promising cost-effective material appropriate for use as an edible coating that contributes to the long-term storage and prolonged freshness of foods.


Assuntos
Celulose/química , Reagentes para Ligações Cruzadas/química , Filmes Comestíveis , Conservação de Alimentos/instrumentação , Fragaria/metabolismo , Nanofibras/química , Cálcio/química , Dióxido de Carbono/química , Cátions , Química/métodos , Conservação de Alimentos/métodos , Íons , Eletricidade Estática
19.
PLoS Comput Biol ; 17(2): e1008767, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33600435

RESUMO

N6-methyladenine (6mA) is an important DNA modification form associated with a wide range of biological processes. Identifying accurately 6mA sites on a genomic scale is crucial for under-standing of 6mA's biological functions. However, the existing experimental techniques for detecting 6mA sites are cost-ineffective, which implies the great need of developing new computational methods for this problem. In this paper, we developed, without requiring any prior knowledge of 6mA and manually crafted sequence features, a deep learning framework named Deep6mA to identify DNA 6mA sites, and its performance is superior to other DNA 6mA prediction tools. Specifically, the 5-fold cross-validation on a benchmark dataset of rice gives the sensitivity and specificity of Deep6mA as 92.96% and 95.06%, respectively, and the overall prediction accuracy is 94%. Importantly, we find that the sequences with 6mA sites share similar patterns across different species. The model trained with rice data predicts well the 6mA sites of other three species: Arabidopsis thaliana, Fragaria vesca and Rosa chinensis with a prediction accuracy over 90%. In addition, we find that (1) 6mA tends to occur at GAGG motifs, which means the sequence near the 6mA site may be conservative; (2) 6mA is enriched in the TATA box of the promoter, which may be the main source of its regulating downstream gene expression.


Assuntos
Adenina/análogos & derivados , Metilação de DNA , DNA/genética , DNA/metabolismo , Aprendizado Profundo , Adenina/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Sequência de Bases , Sítios de Ligação/genética , Biologia Computacional , DNA de Plantas/genética , DNA de Plantas/metabolismo , Bases de Dados de Ácidos Nucleicos , Fragaria/genética , Fragaria/metabolismo , Redes Neurais de Computação , Oryza/genética , Oryza/metabolismo , Rosa/genética , Rosa/metabolismo , Especificidade da Espécie
20.
Plant Cell ; 33(5): 1574-1593, 2021 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-33624824

RESUMO

In contrast to climacteric fruits such as tomato, the knowledge on key regulatory genes controlling the ripening of strawberry, a nonclimacteric fruit, is still limited. NAC transcription factors (TFs) mediate different developmental processes in plants. Here, we identified and characterized Ripening Inducing Factor (FaRIF), a NAC TF that is highly expressed and induced in strawberry receptacles during ripening. Functional analyses based on stable transgenic lines aimed at silencing FaRIF by RNA interference, either from a constitutive promoter or the ripe receptacle-specific EXP2 promoter, as well as overexpression lines showed that FaRIF controls critical ripening-related processes such as fruit softening and pigment and sugar accumulation. Physiological, metabolome, and transcriptome analyses of receptacles of FaRIF-silenced and overexpression lines point to FaRIF as a key regulator of strawberry fruit ripening from early developmental stages, controlling abscisic acid biosynthesis and signaling, cell-wall degradation, and modification, the phenylpropanoid pathway, volatiles production, and the balance of the aerobic/anaerobic metabolism. FaRIF is therefore a target to be modified/edited to control the quality of strawberry fruits.


Assuntos
Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Proteínas de Plantas/metabolismo , Fatores de Transcrição/metabolismo , Ácido Abscísico/metabolismo , Antocianinas/metabolismo , Parede Celular/metabolismo , Metabolismo Energético , Fermentação , Fragaria/genética , Regulação da Expressão Gênica de Plantas , Glicólise , Lignina/metabolismo , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/genética , Propanóis/metabolismo , Interferência de RNA , Fatores de Transcrição/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...