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1.
Food Chem ; 303: 125386, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31473454

RESUMO

The present work investigated the influences of ultrahigh pressure (UHP), ultrasound (US) and their combination (UHP-US) as pretreatments on properties of vacuum-freeze dried strawberry slices. During vacuum-freeze drying, drying duration and total energy consumption of UHP sample, US sample and UHP-US sample was decreased. After the UHP or US pretreatments, a* value (redness), antioxidative substances (total anthocyanin content, total flavonoid content, total phenolic content, diphenyl picrylhydrazyl (DPPH), hydroxyl (-OH) radical-scavenging assay), hardness and cross-section areas of matrix in the dried slices were significantly (P < 0.05) increased. Transverse relaxation times and peak area corresponding to free water in the pretreated samples were obviously decreased, indicating lower mobility in the pretreated samples. As compared with UHP or US individually, UHP in combination with US increased those parameters more pronouncedly. Therefore, UHP and US are promising techniques for the vacuum-freeze drying processing of strawberry products.


Assuntos
Dessecação , Conservação de Alimentos/métodos , Fragaria/química , Frutas/química , Pressão , Ondas Ultrassônicas , Antocianinas/análise , Antioxidantes/análise , Antioxidantes/química , Cor , Flavonoides/análise , Liofilização , Frutas/efeitos da radiação , Fenóis/análise , Vácuo
2.
Food Chem ; 309: 125572, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31732246

RESUMO

A sample preparation method, QuEChERS extraction combined with a magnetic micro dispersive solid phase extraction (MµdSPE), was optimized and evaluated for the trace analysis of 9 brominated flame retardants in red fruit samples (strawberries, blueberries, and raspberries) using gas chromatography-mass spectrometry. Magnetic nanomaterials were used as sorbents providing an extraction of the target compounds. Linearity was established for all the analytes (from 10 to 200 µg kg-1). Seven concentration levels were analyzed with three measurements at each concentration. Linear responses (R2 > 0.99) were obtained, recoveries of all target analytes were within the range of 65-141%, relative standard deviations were <20% at all three spiking levels, while intraday and interday precisions were below 20%. This study demonstrated that the new sample preparation with magnetic nanoparticles could potentially be expanded to extract and pre-concentrate the BFRs in different red fruit samples. The method has been successfully applied to study BFRs in 12 samples from conventional and organic farming.


Assuntos
Retardadores de Chama/análise , Frutas/química , Extração em Fase Sólida/métodos , Mirtilos Azuis (Planta)/química , Fragaria/química , Cromatografia Gasosa-Espectrometria de Massas , Magnetismo , Nanopartículas Metálicas , Rubus/química
3.
Ecotoxicol Environ Saf ; 188: 109842, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31707322

RESUMO

Twelve commercial pesticide formulations containing sixteen active ingredients were applied on greenhouse strawberries at recommended and double doses. The dynamics and dietary risk analysis were investigated. A modified QuEChERS method based on the use of multi-walled carbon nanotubes (MWCNTs) as adsorbent followed by LC-MS/MS and GC-MS/MS detection was utilized for sample analysis. The half-lives of studied pesticides were 4.6-12.6 days and 3.8-15.8 days from two application doses. Dietary levels from the residue concentrations of the individual pesticides at harvest was contrasted with the acceptable daily intake (ADI) and acute reference dose (ARfD). The dietary risk assessment adopt the risk quotient (RQ) for chronic risk and risk probability (RP) for long-term dietary intake risk, respectively. The dietary risk induced by the studied pesticide residues in strawberry was acceptable for consumers except the pesticide fumigants. The pesticide residues at different pre-harvest intervals (PHIs) under greenhouse conditions were compared with the established maximum residue limits (MRLs). To reduce the residue levels and potential safety risk, a longer PHI or reduced application rates should be conducted.


Assuntos
Contaminação de Alimentos/análise , Fragaria/química , Frutas/química , Resíduos de Praguicidas/análise , Cromatografia Líquida , Fragaria/crescimento & desenvolvimento , Frutas/crescimento & desenvolvimento , Nanotubos de Carbono/química , Nível de Efeito Adverso não Observado , Resíduos de Praguicidas/toxicidade , Medição de Risco , Espectrometria de Massas em Tandem
4.
Food Chem ; 309: 125694, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31706673

RESUMO

Despite the numerous studies that have shown a wide range of biological activities to berry fruits, scientific data focusing on modern, rapid and simple extraction methods followed by a clean-up step is still lacking. Therefore, the present work was aimed at investigating the use of a fast one-step solid-liquid extraction procedure followed by a dispersive solid-phase extraction (d-SPE) clean-up step to evaluate the phenolic composition, antioxidant and antiproliferative activities from three of the principal berries found in Brazil, pomegranate (Punica granatum L.), blackberry (Rubus ulmifolius Schott.), and strawberry (Fragaria × ananassa Duch.). Under the optimized extraction conditions, sixteen phenolic compounds were determined by UHPLC-MS/MS analysis and all berry extracts showed antioxidant activity and antiproliferative effects on cervical (HeLa) and colon (HT-29) cancer cells. Overall, these results highlight the importance of the clean-up step for more reliable data in studies of health-promoting proprieties from berry fruits.


Assuntos
Antioxidantes/química , Proliferação de Células/efeitos dos fármacos , Fenóis/análise , Extratos Vegetais/farmacologia , Rosaceae/química , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Fragaria/química , Fragaria/metabolismo , Frutas/química , Frutas/metabolismo , Humanos , Fenóis/química , Fenóis/isolamento & purificação , Extratos Vegetais/química , /metabolismo , Rosaceae/metabolismo , Rubus/química , Rubus/metabolismo , Extração em Fase Sólida , Espectrometria de Massas em Tandem
5.
Food Chem ; 309: 125604, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31689590

RESUMO

In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3). The composition of these polysaccharides was determined. PEPS-2 and PEPS-3 showed outstanding antioxidant activities. PEPS-2 was chosen as a polysaccharide-based coating for strawberry fruit, and its effects on postharvest preservation of the fruit were determined. Strawberries coated with PEPS-2 exhibited significant delay in decay index and lower malondialdehyde, higher soluble solids content, anthocyanin content and vitamin C content, and greater DPPH radical-scavenging activity than uncoated control fruit. The developed method could be beneficial for improving the shelf-life of strawberries.


Assuntos
Conservação de Alimentos/métodos , Fragaria , Frutas , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Pythium/química , Antocianinas/análise , Ácido Ascórbico/análise , Armazenamento de Alimentos , Fragaria/química , Fragaria/fisiologia , Frutas/química , Frutas/fisiologia , Malondialdeído/análise , Polissacarídeos/análise
6.
Food Chem ; 306: 125611, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31606631

RESUMO

Aromatic secondary metabolites are closely related to quality attributes of postharvest fruit. In the present study, 20% CO2 was applied to strawberry fruit to investigate the regulation of elevated CO2 on aromatic secondary metabolites. The results showed that elevated CO2 delayed accumulations of anthocyanins, eugenol and lignin. Phenylalanine and tyrosine, the precursors of the above secondary metabolites, were 18.90% and 35.61%, respectively, lower in CO2-treated fruit compared with the control on day 6. Furthermore, enzyme activities and transcriptional profiles analysis showed pentose phosphate pathway and glycolysis were activated by elevated CO2 whereas the aromatic amino acids (AAAs) pathway was inhibited. These results indicated that elevated CO2 restricted carbon flux into aromatic secondary metabolism by inhibiting the AAAs pathway, leading to the decrease of phenylalanine and tyrosine, and thus, delayed the accumulation of aromatic secondary metabolites. In addition, the effect of elevated CO2 was eliminated after transferred CO2-treated fruit to air.


Assuntos
Fragaria/metabolismo , Antocianinas/análise , Antocianinas/metabolismo , Dióxido de Carbono/química , Eugenol/análise , Eugenol/metabolismo , Fragaria/química , Frutas/química , Frutas/metabolismo , Lignina/análise , Lignina/metabolismo
7.
Food Chem ; 307: 125550, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31639575

RESUMO

Modified atmosphere has widely been evident to contribute to fruit quality maintenance, however the correlation among these quality traits was less known. To explore main factors of elevated atmosphere and reduce the detection indexes, we exposed strawberry to either high O2 (80% O2 + 20% N2) or CO2 (20% CO2 + 20% O2 + 60% N2) atmosphere and compared quality characteristics. It was demonstrated that both atmospheres well maintained the fruit firmness, alleviated weight loss and decay rate. Elevated O2 maintained the polyphenolic contents and cell integrity by significantly decreasing superoxide and hydrogen peroxide levels. PCA analysis implied that HO treatment mainly affected oxygen metabolism while HCO affected carbon metabolism more. Significantly positive correlation was observed between weight loss, anthocyanin content and decay rate in elevated O2 and control groups. This study provided new insights into correlation and difference between impact of elevated O2 and CO2 to postharvest preservation.


Assuntos
Armazenamento de Alimentos , Fragaria/química , Oxigênio/química , Antocianinas/metabolismo , Dióxido de Carbono/química , Fragaria/metabolismo , Frutas/química , Peróxido de Hidrogênio/metabolismo , Superóxidos/metabolismo
8.
Chemosphere ; 238: 124651, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31472346

RESUMO

A harbour sediment, previously remediated, was tested for soilless strawberry cultivation (Camarosa and Monterey cultivars), as an innovative, cost-effective and environment-friendly approach of sediment management. Sediments were tested as such (TS100) and mixed 1/1 (v/v) with a peat-based commercial substrate (TS50), using the peat-based medium as control (TS0). Substrates were characterized for some physicochemical properties (e.g. density, porosity and water capacity). Minerals (P, Ca, K, Na and Fe), heavy metals (Cu, Zn, Mn, Ni, Cr, Pb and Cd), aliphatic hydrocarbons (C > 12), polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), dibenzodioxins and dibenzofurans were analysed in substrates and fruits. Sugars and organic acids, including the ascorbic, were also determined in fruits, as quality indicators. Notwithstanding remediation, sediments showed concentrations of Zn (206 mg kg-1), C > 12 (86 mg kg-1) and PAHs (47 mg kg-1) exceeding the limits established by the Italian L.D. 152/2006, regulating the contamination of soil in green areas, thus making its relocation in the environment not permitted as such. No evidence of fruit contamination by Cr, Pb and Cd was highlighted. Moreover, Cu, Zn and Ni fruit concentrations were comparable among treatments. Conversely, Mn showed statistically higher concentrations in TS0 fruits (56-57 mg kg-1) compared to those grown in sediment-based substrates (8-20 mg kg-1). Among organic contaminants, only dioxin-like PCBs were determined in fruits, at toxic equivalent concentrations fourfold lower than the limit established by the European Union. TS100 fruits showed a yield reduction from 40 to 70% for Camarosa and Monterey, but higher sugar and ascorbic acid contents.


Assuntos
Produtos Agrícolas/normas , Monitoramento Ambiental , Fragaria/metabolismo , Frutas/química , Sedimentos Geológicos/química , Produtos Agrícolas/química , Produtos Agrícolas/crescimento & desenvolvimento , Fragaria/química , Itália , Metais Pesados/análise , Minerais/análise , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Poluentes do Solo/química
9.
J Sci Food Agric ; 100(5): 1949-1961, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31846082

RESUMO

BACKGROUND: The postharvest application of pectic-oligosaccharides (POS) as an elicitor to improve the postharvest shelf-life and nutritional quality by stimulating natural defense mechanisms in strawberries was studied. Strawberries (cv. Festival) were treated with POS (at 0, 2, 5, and 9 g L-1 ) and evaluated for firmness, weight loss, color, soluble solids, titratable acidity (TA), total phenolic and anthocyanin content, antioxidant capacity, decay, and some defense-related enzyme activity during storage at 2 ± 0.5 °C for 14 days. RESULTS: Treatment with POS significantly delayed (P < 0.05) strawberry decay, and reduced the water loss and softening of fruit during storage. Strawberries treated with POS showed a significant increase in total phenolic and anthocyanin content, and antioxidant capacity when compared with controls. Interestingly, POS induced higher activity of phenylalanine ammonia-lyase (PAL), chitinase, and ß-1,3-glucanase in strawberries. Compared to the control, the activity of enzymes was markedly increased in fruit treated with all tested POS concentrations, particularly chitinase, and ß-1,3-glucanase activities, but 5 and 9 g L-1 POS were the most effective treatments for maintaining the quality attributes and improving anthocyanin accumulation and antioxidant capacity of strawberries. CONCLUSION: These findings suggest that POS treatment could potentially be applied to maintain quality attributes, reduce decay, and further enrich the anthocyanin content and antioxidant capacity of strawberries during postharvest storage. The results also suggest that the positive effects of POS on strawberries could be associated with the rapid accumulation of chitinase and ß-1,3-glucanase activities, and the increase of PAL enzyme activity leading to the synthesis and accumulation of anthocyanins. © 2019 Society of Chemical Industry.


Assuntos
Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Fragaria/efeitos dos fármacos , Frutas/química , Oligossacarídeos/farmacologia , Pectinas/farmacologia , Antocianinas/análise , Antioxidantes/análise , Armazenamento de Alimentos , Fragaria/química , Frutas/efeitos dos fármacos , Valor Nutritivo , Fenóis/análise , Fenilalanina Amônia-Liase/análise
10.
J Sci Food Agric ; 100(5): 1980-1989, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31849062

RESUMO

BACKGROUND: In this study, an infrared-based prediction method was developed for easy, fast and non-destructive detection of pesticide residue levels measured by reference analysis in strawberry (Fragaria × ananassa Duch, cv. Albion) samples using near-infrared spectroscopy and demonstrating its potential alternative or complementary use instead of traditional pesticide determination methods. Strawberries of Albion variety, which were supplied directly from greenhouses, were used as the study material. A total of 60 batch sample groups, each consisting of eight strawberries, was formed, and each group was treated with a commercial pesticide at different concentrations (26.7% boscalid + 6.7% pyraclostrobin) and varying residual levels were obtained in strawberry batches. The strawberry samples with pesticide residuals were used both to collect near-infrared spectra and to determine reference pesticide levels, applying QuEChERS (quick, easy, cheap, rugged, safe) extraction, followed by liquid chromatographic-mass spectrometric analysis. RESULTS AND CONCLUSION: Partial least squares regression (PLSR) models were developed for boscalid and pyraclostrobin active substances. During model development, the samples were randomly divided into two groups as calibration (n = 48) and validation (n = 12) sets. A calibration model was developed for each active substance, and then the models were validated using cross-validation and external sets. Performance evaluation of the PLSR models was evaluated based on the residual predictive deviation (RPD) of each model. An RPD of 2.28 was obtained for boscalid, while it was 2.31 for pyraclostrobin. These results indicate that the developed models have reasonable predictive power. © 2019 Society of Chemical Industry.


Assuntos
Contaminação de Alimentos/análise , Fragaria/química , Resíduos de Praguicidas/análise , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Compostos de Bifenilo/análise , Frutas/química , Fungicidas Industriais/análise , Análise dos Mínimos Quadrados , Niacinamida/análogos & derivados , Niacinamida/análise , Estrobilurinas/análise
11.
J Food Sci ; 85(1): 50-56, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31880331

RESUMO

The aim of this work was to study the influence of ι- and κ-carrageenan addition to strawberry sorbet prepared by various freezing methods (cryostat and conventional freezer) on freezing kinetics and ice crystals structure. Four variants of strawberry-based sorbet were prepared: with no additive, with 0.1% addition of ι- and κ-carrageenan, and 1:1 blend of both. Freezing curves and freezing time, as well as the size and morphology of ice crystals, were analyzed. The addition of carrageenan stabilizers resulted in freezing process elongation. Reference samples were characterized by different dynamics of the freezing process. The addition of carrageenan increased the final temperature of the samples, compared to the reference sample (even 2.6 °C of difference). Additives reduced ice crystals growth. The most efficient was mixture of both fractions of carrageenan--the samples in this variant frozen in conventional freezer were characterized by the smallest ice crystals diameter--average 5.44 µm, while for the reference sample it was 17.79 µm. PRACTICAL APPLICATION: This research can give a new path for the carrageenan's hydrolysates application--not only for dairy products. Iota carrageenan fraction was recommended for dairy products according to its water-holding capacity in the presence of calcium ions. This study showed that mix of ι- and κ-carrageenan could bring positive results to such product as sorbet without any milk proteins addition. This experiment data could also be helpful when optimizing frozen desserts production in small gastronomy--especially the temperature and time of freezing modifications.


Assuntos
Carragenina/análise , Aditivos Alimentares/análise , Fragaria/química , Frutas/química , Extratos Vegetais/química , Conservação de Alimentos/métodos , Congelamento , Gelo/análise , Temperatura Ambiente , Água/química
12.
Food Funct ; 10(11): 6987-6998, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31637390

RESUMO

Cardiovascular disease (CVD) is the greatest cause of premature death and disability globally. Consequently, numerous therapeutic strategies have been developed in order to prevent the onset of adverse cardiovascular events including nutritional approaches. This includes strawberries as they have a high oxidant and micronutrient content, so we examined the extent to which dietary supplementation impacts on CVD risk factors. A comprehensive literature search without any limitation on language was conducted using the following bibliographical databases: ISI Web of Science, Scopus, PubMed and Cochrane Library. Search was conducted between 1985 and February 2019. The mean difference (MD) of the reported effects was calculated using a random effect model. A total of 20 groups from 14 clinical trials were included for final analysis. The pooled effect size showed that strawberry supplementation decreased circulating oxidized LDL (MD = -5.8 ng ml-1, p = 0.012), malondialdehyde (0.309 µmol L-1, p = 0.002), C-reactive protein (MD = -0.472 mg L-1, p = 0.003), total cholesterol (MD = -6.49 mg dL-1, p = 0.019), and diastolic blood pressure (MD = -2.220 mmHg, p = 0.033). It also demonstrated raised fasting blood sugar (MD = 2.083 mg dl-1; p = 0.040), but had no effect on other CVD risk factors examined. Dietary supplementation with strawberries improved specific CVD risk factors, suggesting that larger well-designed, adequately powered, and longer-term follow up studies should now be undertaken.


Assuntos
Doenças Cardiovasculares/dietoterapia , Fragaria/metabolismo , Adulto , Pressão Sanguínea , Doenças Cardiovasculares/genética , Doenças Cardiovasculares/metabolismo , Doenças Cardiovasculares/fisiopatologia , HDL-Colesterol/metabolismo , LDL-Colesterol/metabolismo , Feminino , Fragaria/química , Frutas/química , Frutas/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados Aleatórios como Assunto , Adulto Jovem
13.
Food Funct ; 10(11): 7103-7120, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31621765

RESUMO

The aim of this study was the evaluation of the effects of strawberry anthocyanin extract treatment on two in vitro models of murine breast cancer cell lines, in an attempt to detect a specific pathway (AMP-activated protein kinase or AMPK) through which strawberries exert their anticancer activity. The anticancer activity of purified anthocyanin extracts from an Alba cultivar on two murine cancer cell lines, N202/1A (with high levels of the HER2/neu oncogene) and N202/1E (with low levels of the HER2/neu oncogene), was evaluated after 48 and 72 h of treatment. The cell viability and apoptosis, intracellular ROS rates, and cell oxidative damage were assessed. Western blot assays were performed to analyze the expression of several proteins related to apoptosis, autophagy, metastasis, the oxidative status, mitochondrial functionality, and the AMPK pathway. This study demonstrated that the anthocyanin extract of Alba strawberry shows an antiproliferative effect on cancer cells, through the induction of apoptosis and oxidative stress, by stimulating different molecular pathways. This study is one of the first studies that have tried to deepen the understanding of a candidate pathway for the explanation of the effects of strawberry on cancer cells. A relationship between the AMPK pathway and the anticancer effects of strawberries was demonstrated.


Assuntos
Antocianinas/farmacologia , Neoplasias da Mama/fisiopatologia , Fragaria/química , Extratos Vegetais/farmacologia , Proteínas Quinases Ativadas por AMP/genética , Proteínas Quinases Ativadas por AMP/metabolismo , Animais , Antocianinas/isolamento & purificação , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Neoplasias da Mama/genética , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Feminino , Humanos , Camundongos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Espécies Reativas de Oxigênio/metabolismo
14.
Molecules ; 24(18)2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31547424

RESUMO

The Argentinean Patagonia berries Berberis microphylla, Berberis darwinii, and Fragaria chiloensis ssp. chiloensis f. patagonica were investigated for their polyphenol content and composition by means of liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry. The in vitro antioxidant activity and inhibition of metabolic syndrome-associated enzymes (α-glucosidase, α-amylase, and lipase) of the fruit extracts was assessed. The most complex polyphenol profile was found in the Berberis samples, with 10 anthocyanins, 27 hydroxycinnamic acids, 3 proanthocyanidins, 2 flavan-3-ol, and 22 flavonols. Fragaria presented four anthocyanins, nine ellagitannins, two proanthocyanidin dimers, one flavan-3-ol, and five flavonols. The Berberis samples showed the best antioxidant capacity, while Fragaria displayed better activity against α-glucosidase and lipase. The phenolic content and composition of the Argentinean Patagonia berries was similar to that reported for Chilean samples but with some chemical differences between Eastern (Argentina) and Western (Chile) Patagonia. The data obtained supports the consumption of these berries as sources of beneficial polyphenols.


Assuntos
Antioxidantes/farmacologia , Berberis/química , Fragaria/química , Polifenóis/análise , Polifenóis/farmacologia , Antocianinas/análise , Antioxidantes/análise , Argentina , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos/análise , Inibidores de Glicosídeo Hidrolases/farmacologia , Humanos , Taninos Hidrolisáveis/análise , Síndrome Metabólica/enzimologia , Extratos Vegetais/análise , Extratos Vegetais/farmacologia , Espectrometria de Massas por Ionização por Electrospray , alfa-Amilases/antagonistas & inibidores
15.
J Sci Food Agric ; 99(15): 6973-6980, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31414485

RESUMO

BACKGROUND: Plants activate defense mechanisms to cope with adverse environmental conditions, leading to the accumulation and / or depletion of general and specialized metabolites. In this study, a multiplatform untargeted metabolomics strategy was employed to evaluate metabolic changes in strawberry fruit of cv. Camarosa grown under osmotic stress conditions. Liquid chromatography-mass spectrometry (LC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) data from strawberries grown under two water-deficit conditions, irrigated at 95% crop evapotranspiration (ETc) and 85% ETc, and one excess salt condition with a 80 mmol L-1 NaCl solution, were analyzed to determine treatment effects on fruit metabolism. RESULTS: Multivariate principal component analysis, orthogonal projections to latent structures - discriminant analysis (OPLS-DA), and univariate statistical analyses were applied to the data set. While multivariate analyses showed group separation by treatment, T-tests and fold change revealed 12 metabolites differentially accumulated in strawberries from different treatments - among them phenolic compounds, glycerophospholipids, phytosterols, carbohydrates, and an aromatic amino acid. CONCLUSION: Untargeted metabolomic analysis allowed for the annotation of compounds differentially accumulated in strawberry fruit from plants grown under osmotic stress and non-stressed plants. The metabolic disturbance in plants under stress involved metabolites associated with the inhibition of reactive oxygen species and cell-wall and membrane lipid biosynthesis, which might serve as osmotic stress biomarkers. © 2019 Society of Chemical Industry.


Assuntos
Fragaria/química , Frutas/metabolismo , Cromatografia Líquida , Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Frutas/química , Frutas/crescimento & desenvolvimento , Cromatografia Gasosa-Espectrometria de Massas , Metabolômica , Pressão Osmótica , Cloreto de Sódio/análise , Cloreto de Sódio/metabolismo , Espectrometria de Massas em Tandem , Água/análise , Água/metabolismo
16.
Nutrients ; 11(7)2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-31269727

RESUMO

Strawberries and blueberries are two of the most commonly consumed berries. Berries, in general, are characterized by their highly nutritive compounds, including minerals, vitamins, fatty acids, and dietary fiber, as well as their high content and wide diversity of bioactive compounds, such as phenolic compounds and organic acids. These bioactive compounds have been associated with protective effects against chronic diseases, such as cardiovascular disease, cancer, Alzheimer's and other disorders. In this paper 16 human intervention studies investigating the beneficial health effects of dietary strawberry or blueberry consumption on inflammation, cardiovascular disease or cognitive function and mental health are reviewed.


Assuntos
Antocianinas/análise , Mirtilos Azuis (Planta)/química , Fragaria/química , Frutas/química , Polifenóis/análise , Ensaios Clínicos como Assunto , Nível de Saúde , Humanos , Valor Nutritivo , Recomendações Nutricionais , Comportamento de Redução do Risco
17.
J Sci Food Agric ; 99(14): 6562-6571, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31321778

RESUMO

BACKGROUND: Excessive reactive oxygen species (ROS) may attack biological macromolecules and induce oxidative stress. The inhibition by ascorbic acid (AsA) on oxidative damage has been reported in fruits, while the barrier effect of ceramide has also been proven. However, there are few reports about the effects of ceramide-AsA interactions to enhance storability and boost antioxidant systems in fruits during storage. This study was conducted to study the synergistic effects of AsA in combination with ceramide on the quality of postharvest strawberry (Fragaria anannasa cv. Tianbao). RESULTS: Treatment with 100 mg L-1 AsA plus 1.2 mmol L-1 ceramide significantly delayed the rot of strawberries, reduced the water loss and the contents of ROS, malonaldehyde (MDA), and proline, however, increased the contents of total flavonoids, total phenols, and anthocyanins compared with other treatments. Also, treatment with 100 mg L-1 AsA plus 1.2 mmol L-1 ceramide significantly increased the activities of peroxidase (POD) and superoxide dismutase (SOD) but inhibited the activity of polyphenol oxidase (PPO). CONCLUSION: It is suggested that treatment with 100 mg L-1 AsA plus 1.2 mmol L-1 ceramide could significantly reduce the oxidative damage and maintain the storage quality of strawberries during storage by enhancing the antioxidant systems. © 2019 Society of Chemical Industry.


Assuntos
Antioxidantes/metabolismo , Ácido Ascórbico/farmacologia , Ceramidas/farmacologia , Conservação de Alimentos/métodos , Conservantes de Alimentos/farmacologia , Fragaria/efeitos dos fármacos , Catalase/metabolismo , Sinergismo Farmacológico , Armazenamento de Alimentos , Fragaria/química , Fragaria/metabolismo , Frutas/química , Frutas/efeitos dos fármacos , Frutas/metabolismo , Malondialdeído/metabolismo , Estresse Oxidativo , Proteínas de Plantas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
18.
Food Chem ; 299: 125138, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31302430

RESUMO

As an environmentally friendly approach for fruit quality improvement, the effect of preharvest UV-C on the physiology of strawberry fruit during postharvest storage remains to be assessed. Strawberry fruit developed with supplementary UV-C were stored at room temperature for 2 weeks. Preharvest UV-C attenuated fruit postharvest senescence and altered phytochemicals composition. Higher ester titer was found in the treated fruit at harvest, whereas higher terpene and furanone contents were detected after 72 h of storage. At harvest, polyphenolics accumulated to a higher level in UV-C group, but the difference disappeared after 24 h of storage. Meanwhile, the intrinsic level of abscisic acid and the expressions of FaPYR1, SnRK2, and FaASR in the UV-C-treated fruit was enhanced at harvest but returned to a lower level as storage proceeded. This study highlights the time-dependent effect of preharvest UV-C on strawberry fruit postharvest biochemical indexes and the possible involvement of abscisic acid signaling factors.


Assuntos
Ácido Abscísico/metabolismo , Armazenamento de Alimentos/métodos , Fragaria/fisiologia , Frutas/fisiologia , Compostos Fitoquímicos/metabolismo , Ácido Abscísico/genética , Fragaria/química , Fragaria/efeitos da radiação , Frutas/química , Frutas/metabolismo , Frutas/efeitos da radiação , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Compostos Fitoquímicos/análise , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Polifenóis/análise , Polifenóis/metabolismo , Terpenos/análise , Terpenos/metabolismo , Raios Ultravioleta
19.
J Agric Food Chem ; 67(33): 9265-9276, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31361479

RESUMO

Fungal infections significantly alter the emissions of volatile organic compounds (VOCs) by plants, but the mechanisms for VOCs affecting fungal infections of plants remain largely unknown. Here, we found that infection by Botrytis cinerea upregulated linalool production by strawberries and fumigation with linalool was able to inhibit the infection of fruits by the fungus. Linalool treatment downregulated the expression of rate-limiting enzymes in the ergosterol biosynthesis pathway, and this reduced the ergosterol content in the fungi cell membrane and impaired membrane integrity. Linalool treatment also caused damage to mitochondrial membranes by collapsing mitochondrial membrane potential and also downregulated genes involved in adenosine triphosphate (ATP) production, resulting in a significant decrease in the ATP content. Linalool treatment increased the levels of reactive oxygen species (ROS), in response to which the treated fungal cells produced more of the ROS scavenger pyruvate. RNA-Seq and proteomic analysis data showed that linalool treatment slowed the rates of transcription and translation.


Assuntos
Botrytis/efeitos dos fármacos , Fragaria/metabolismo , Frutas/microbiologia , Monoterpenos/metabolismo , Doenças das Plantas/microbiologia , Compostos Orgânicos Voláteis/metabolismo , Trifosfato de Adenosina/metabolismo , Botrytis/crescimento & desenvolvimento , Fragaria/química , Fragaria/microbiologia , Frutas/química , Frutas/metabolismo , Interações Hospedeiro-Patógeno , Membranas Mitocondriais/efeitos dos fármacos , Membranas Mitocondriais/metabolismo , Monoterpenos/farmacologia , Doenças das Plantas/prevenção & controle , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteômica , Espécies Reativas de Oxigênio/metabolismo , Compostos Orgânicos Voláteis/farmacologia
20.
BMC Complement Altern Med ; 19(1): 140, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31221152

RESUMO

BACKGROUND: Cholera is one of the most deadly diarrheal diseases that require new treatments. We investigated the neutralization of cholera toxin by five plant extracts obtained from the Rosaceae family that have been traditionally used in Poland to treat diarrhea (of unknown origin). METHODS: Hot water extracts were prepared from the dried plant materials and lyophilized before phytochemical analysis and assessment of antimicrobial activity using microdilution assays. The ability of the plant extracts to neutralize cholera toxin was analyzed by measurement of cAMP levels in cell cultures, enzyme-linked immunosorbent assay and electrophoresis, as well as flow cytometry and fluorescence microscopy studies of fluorescent-labeled cholera toxins with cultured human fibroblasts. RESULTS: The antimicrobial assays displayed modest bacteriostatic potentials. We found that the plant extracts modulate the effects of cholera toxin on intracellular cAMP levels. Three plant extracts (Agrimonia eupatoria L., Rubus fruticosus L., Fragaria vesca L.) suppressed the binding of subunit B of cholera toxin to the cell surface and immobilized ganglioside GM1 while two others (Rubus idaeus L., Rosa.canina L.) interfered with the toxin internalization process. CONCLUSIONS: The traditional application of the Rosaceae plant infusions for diarrhea appears relevant to cholera, slowing the growth of pathogenic bacteria and either inhibiting the binding of cholera toxin to receptors or blocking toxin internalization. The analyzed plant extracts are potential complements to standard antibiotic treatment and Oral Rehydration Therapy for the treatment of cholera.


Assuntos
Antibacterianos/farmacologia , Toxina da Cólera/toxicidade , Cólera/microbiologia , Extratos Vegetais/farmacologia , Rosaceae/química , Agrimonia/química , Antibacterianos/química , Linhagem Celular , Cólera/tratamento farmacológico , Cólera/metabolismo , Toxina da Cólera/metabolismo , Fragaria/química , Gangliosídeo G(M1)/metabolismo , Humanos , Extratos Vegetais/química , Rubus/química , Vibrio cholerae/efeitos dos fármacos , Vibrio cholerae/metabolismo
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