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1.
Zhongguo Zhong Yao Za Zhi ; 44(3): 448-453, 2019 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30989906

RESUMO

A new variety "Zhebei 3(Zhejiao Pharmaceutical 2018002)" was selected and bred from multi seeded Fritillaria thunbergii mutants by systematic breeding method. From 2012 to 2016, the traits assessment, disease resistance appraisal, plot ratios and regional trials of the variety were continuously carried out. The results showed that "Zhebei 3" emerged early and had late seedlings. The average growth period was about 100 days, which was 6 days and 12 days higher than the "Zhebei 1" and "Zhebei 2". The average yield was 5 095.5 kg·hm~(-2), which was 14.42% and 17.71% higher than of the control respectively. The average proliferation rate of bulbs was 261.2%, which was 37.46% and 31.58% higher than that of the control, respectively. The propagation coefficient of bulbs was about 1∶2.6, and the total amount of peimine and peiminine was 0.172 2%, which was 4.49% and 29.47% higher than the control, respectively. The identification of disease resistance showed that it was resistance to bulb stem(soft) rot, better than the control. "Zhebei 3" has stable characters, high yield, good quality, strong disease resistance, and moderate propagation coefficient which is suitable for planting in Zhejiang province.


Assuntos
Fritillaria/crescimento & desenvolvimento , Melhoramento Vegetal , Resistência à Doença , Doenças das Plantas , Raízes de Plantas
2.
Genomics ; 111(6): 1360-1372, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-30248489

RESUMO

Crown imperial (CI) has been used in traditional medicine. Today it is known that such beneficial effects are due to its richness in steroidal alkaloids (SA). Using de novo transcriptomics, orthologues/paralogues finder, phylogenetic analysis and tissue- and developmental stage-specific expression analysis, we identified ten genes and several TFs involved in the biosynthesis of SA in CI. The comparative analysis of ten genes expression profiles revealed the possibility of their co-regulation, which may imply the possibility of their organization in metabolic gene clusters. Having in mind convergent evolution of steroidal biosynthetic pathways in flowering plants and records of convergent evolution of specific proteins, observed expression patterns open a reasonable interest to investigate the possibility of the existence of genes cluster organization in SA pathway in the family Liliaceae or at least in some species of genus Fritillaria. Obtained results support transcriptomics as useful approach in elucidating genes underlying complex biochemical pathways.


Assuntos
Alcaloides/biossíntese , Fritillaria/genética , Fritillaria/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Esteroides/biossíntese , Transcriptoma , Alcaloides/genética , Vias Biossintéticas , Biologia Computacional , Fritillaria/crescimento & desenvolvimento , Filogenia , Proteínas de Plantas/metabolismo , Esteroides/metabolismo
3.
Phytochem Anal ; 29(3): 290-299, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29336082

RESUMO

INTRODUCTION: Bulbus fritillariae cirrhosae (known as Chuan bèi mǔ in China, BFC) contain fritillaria steroidal alkaloids as the bioactive ingredients and are widely used as traditional Chinese medicine for the treatment of cough and phlegm. Due to limited wild resources, the cultivated species are becoming predominantly used in Chinese traditional medicine markets. OBJECTIVE: To assess the impact of different growth stages on the alkaloids of cultivated BFC and establish a reference for quality control and guidance for appropriate harvesting practices. METHODS: The ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) metabolomic strategy was applied to determine potential chemical markers for the discrimination and quality control of cultivated BFC in different growth stages. The molecular feature extraction and multivariate statistical analysis were applied to alkaloid extraction and full metabolomic profiling of cultivated BFC for classification and marker compound characterisation. RESULT: This approach allowed the establishment of a fast and efficient comparative multivariate analysis of the metabolite composition of 42 samples covering growth of cultivated BFC ranging in age from one to seven years old. Four alkaloid compounds were identified in cultivated BFC based on accurate mass, retention time, and MS/MS fragments. These compounds may be used as potential chemical markers for the classification and discrimination of cultivated BFC samples indifferent growth stages. CONCLUSIONS: The proposed analytical method in combination with multivariate statistical analysis comprised a useful and powerful strategy to explore the chemical ingredients and transforming mechanisms of cultivated BFC and for quality evaluation and control. Copyright © 2018 John Wiley & Sons, Ltd.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Fritillaria/metabolismo , Metabolômica , Espectrometria de Massas em Tandem/métodos , Alcaloides/isolamento & purificação , Fritillaria/crescimento & desenvolvimento , Medicina Tradicional Chinesa , Análise Multivariada , Controle de Qualidade , Padrões de Referência
4.
Zhongguo Zhong Yao Za Zhi ; 41(17): 3194-3201, 2016 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-28920370

RESUMO

The major contributing factors for growth of endangered medicinal plants of Fritillariae Cirrhosae Bulbus were screened on the GIS platform by using the MaxEnt model, and spatial distribution data of the medicine quality suitability were generated by geostatistics interpolation based on reported measured data of ecology and quality suitability assessment. On this basis, a functional production cultivation regionalization with high feasibility and operability were formatted for protection, wild monitoring, and cultivation of this plant by fuzzy superposition of spatial suitability data of ecology and quality, as well as integrated with land use and cover data. Therefore, a novel assessment and regionalization method were presented for ecology, growth and quality suitability of the Chinese traditional medicinal plants. This method is expected to overcome shortage of traditional regionalization methods difficult to distinguish the contribution of ecological factors and quality factors, which provide an innovative theory and methodology for regionalization, and is helpful to practical application of wild resource protection, monitoring, and commercialization cultivation for traditional Chinese medicinal plants.


Assuntos
Fritillaria/crescimento & desenvolvimento , China , Ecologia , Sistemas de Informação Geográfica , Plantas Medicinais/crescimento & desenvolvimento
5.
Ontogenez ; 46(4): 259-66, 2015.
Artigo em Russo | MEDLINE | ID: mdl-26480485

RESUMO

Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initiation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM 6-benzylaminopurine and 2 µM α-naphthaleneacetic acid. Optimization of propagation stage was carried out using obtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate were observed on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 pcs./explant, respectively) and hormone-free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has been established that the ad- dition of growth regulators on the stage of cultivation does not cause increased morphogenic response but contributes to the accelerated initiation and development of microbulbs. Morphological and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro development of F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.


Assuntos
Fritillaria/crescimento & desenvolvimento , Morfogênese , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/crescimento & desenvolvimento , Regeneração , Compostos de Benzil , Meios de Cultura , Fritillaria/fisiologia , Técnicas In Vitro , Cinetina/farmacologia , Morfogênese/fisiologia , Ácidos Naftalenoacéticos/farmacologia , Reguladores de Crescimento de Planta/farmacologia , Purinas , Regeneração/fisiologia , Federação Russa
6.
Zhongguo Zhong Yao Za Zhi ; 40(3): 421-3, 2015 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-26084163

RESUMO

In order to study the accumulation of Fritillaria thunbergii cultivar, peimine content in Xiaye, Kuanye, Duozi and Xiaosanzi bulbs of different sizes and parts was determined by HPLC-ELSE. The results indicated that the peimine content varied significantly with the cultivar type, the size and part of bulb. The distribution laws of peimine were as follow: Xiaosanzi > Duozi > Xiaye > Kuanye, small-size bulb > big-size bulb, core bud > scale. The peimine yield per plant in Duozi was the highest.


Assuntos
Cevanas/análise , Fritillaria/química , Cromatografia Líquida de Alta Pressão , Fritillaria/crescimento & desenvolvimento
7.
Zhong Yao Cai ; 37(6): 931-4, 2014 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-25470953

RESUMO

OBJECTIVE: To study the effects of different hormone induced bulb of Fritillaria cirrhosa seedling leaves, in order to provide an effective way to expant Fritillaria cirrhosa source. METHODS: Fritillaria cirrhosa seedling leaves were used as explants, orthogonal test was carried out to study the medium consisting of 6-BA, NAA, 2, 4-D and KT, and proliferation induced by direct bulb. Induction rate and average number of bulb producing was treated as the assessment indices, and the content of alkaloid of bulb was determined. RESULTS: The best medium formula for Fritillaria cirrhosa tissue culture seedling was MS+6-BA 2.0 mg/L +NAA 1.0mg/L +KT 1.0mg/L, the bulb induction rate was 83.37% and the average number of bulb was 17.27. The alkaloid content of induced bulb was 0.389%, which was 1.64 times that of wild Fritillaria cirrhosa bulb. CONCLUSION: This study provides a simple and rapid mehod for the production of tissue culture of Fritillaria cirrhosa bulb. It is useful for reasonable development and utilization of Fritillaria cirrhosa resources, as well as improvement of the quality of medicinal materials.


Assuntos
Fritillaria/efeitos dos fármacos , Reguladores de Crescimento de Planta/farmacologia , Alcaloides , Fritillaria/crescimento & desenvolvimento , Raízes de Plantas
8.
Plant Biol (Stuttg) ; 14(2): 285-94, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21972995

RESUMO

For 4 years we studied pollination biology and breeding system of the critically endangered, Red List plant Fritillaria meleagris L. (Liliaceae), in the larger of the two remaining populations of the plant in SE Poland. Our observations indicated that, contrary to literature data, the species is not dichogamous nor is it obligatorily out-crossing. Selfing, although rare in natural populations, results in fully developed seeds. Flowers are visited by several insect species, mostly social and solitary bees. In spite of extremely low visitation rates to this early spring-flowering plant, the species is not pollen limited. Although the largest pollen loads are transferred by solitary bees, the key pollinators are bumblebees (mostly the most common species, Bombus terrestris and B. lapidarius) due to their seasonal and floral constancy, and tolerance of bad weather conditions. The current decline of the studied population seems not to be related to the species' pollination or breeding systems but to plant habitat loss. It is suggested, however, that in smaller populations, the species' dependence on generally rare pollinators and largely out-crossed breeding system may accelerate local extinction.


Assuntos
Abelhas/fisiologia , Fritillaria/fisiologia , Polinização/fisiologia , Animais , Cruzamento , Ecossistema , Flores/crescimento & desenvolvimento , Flores/fisiologia , Fritillaria/crescimento & desenvolvimento , Polônia , Dinâmica Populacional , Reprodução/fisiologia , Sementes/crescimento & desenvolvimento , Sementes/fisiologia
9.
Zhong Yao Cai ; 34(7): 1034-7, 2011 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22066394

RESUMO

OBJECTIVE: To study the content of peimisine and total alkaloids in Fritllaria taipaiensis of different growing stage. METHODS: High performance liquid chromatography coupled with evaporative light scattering detector was employed, and peimisine was separated and quantified upon an Agilent Extend C18 (250 mm x 4.0 mm, 5 microm), using acetonitrile-water-diethylamine as the mobile phase at the flow rate of 1.0 mL min(-1); UV spectrophotometry was used for the determination of total alkaloids. RESULTS: During from two to three years period, the content of peimisine in F. taipaiensis increased with growing stage, from four to six years, it decreased with growing stage; From two to four years, the content of total alkaloids in F. taipaiensis increased with growing stage, from five to six years, it decreased with growing stage. CONCLUSION: It is suitable to harvest F. taipaienssi of four years growing stage.


Assuntos
Alcaloides/análise , Cromatografia Líquida de Alta Pressão/métodos , Fritillaria/química , Plantas Medicinais/química , Medicamentos de Ervas Chinesas/análise , Fritillaria/crescimento & desenvolvimento , Plantas Medicinais/crescimento & desenvolvimento , Controle de Qualidade , Reprodutibilidade dos Testes , Espalhamento de Radiação , Estações do Ano , Fatores de Tempo
10.
Zhong Yao Cai ; 34(2): 183-6, 2011 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-21823471

RESUMO

OBJECTIVE: Set up Fritillaria cirrhosa cell mass suspension culture system to rapidly screen the best culture conditions for cell mass proliferation and hormone combination. METHODS: Using MS medium as the basic medium, the impact of inoculum size, hormone combination, growth regulators for Fritillaria cirrhosa cell mass suspension culture were compared, and also the growth of cell mass at different culture conditions was compared, and the total alkaloids content in proliferative cell mass was measured. RESULTS AND CONCLUSION: Fritillaria cirrhosa grow significantly faster in cell mass suspension culture than in the solid culture. The total alkaloid content in cell mass is higher than commercial and wild bulb of Fritillaria cirrhosa. The optimal inoculum size for cell mass suspension culture is 30 g/L and the optimal culture media is MS +6-BA 2.0 mg/L + NAA 0.2 mg/L.


Assuntos
Alcaloides/metabolismo , Técnicas de Cultura de Células/métodos , Fritillaria/citologia , Fritillaria/metabolismo , Reguladores de Crescimento de Planta/farmacologia , Proliferação de Células/efeitos dos fármacos , Células Cultivadas/metabolismo , Meios de Cultura , Fritillaria/crescimento & desenvolvimento , Reguladores de Crescimento de Planta/metabolismo , Ribavirina/metabolismo , Ribavirina/farmacologia , Espectrofotometria
11.
Int J Mol Sci ; 12(6): 3770-85, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21747705

RESUMO

To explore rhizosphere effects, geographical differences and their effects on the bacterial community associated with the geoherb Fritillaria thunbergii, some physicochemical properties of soil samples (3 sampling sites × 2 habitats (rhizosphere and bulk soil)) were measured and the soil bacterial community detected by PCR-denaturing gradient gel electrophoresis (DGGE). Among the three regions, soil pH varied between 4.48 and 7.73 indicating that F. thunbergii could grow both in acid and slightly alkaline soil. As the authentic Dao-di producing area, Ningbo showed the highest soil quality with the highest content of organic matter (OM) (2.46%), phosphatase (268 mg kg(-1) 24 h(-1)) and urease activity (1481 mg kg(-1) 24 h(-1)). In comparison with the bulk soil, pH, organic carbon content, and phosphatase and urease activities were all lower in the rhizosphere, suggesting that the roots may secrete some unique metabolites in root exudates. Statistical analyses showed that soil properties of Ningbo and Panan in Zhejiang province were more similar to each other than those in Nantong in Jiangsu province. In addition, PCR-DGGE analysis showed that main bacterial population identified in F. thunbergii was proteobacteria (18 bands, 55%), acidobacteria (4, 12%), actinobacteria (4, 12%) and bacterioidetes (6, 18%). Overall, soil properties and microbial communities varied not only between the rhizosphere and bulk soil but also among the three regions. We suggest that the plant, together with the soil properties, cooperatively shape the structure of the rhizosphere bacteria, and that the soil properties have a close relationship with the geoherbalism of F. thunbergii.


Assuntos
Fritillaria/crescimento & desenvolvimento , Rizosfera , Microbiologia do Solo , Solo/química , Bactérias/genética , Bactérias/isolamento & purificação , DNA Bacteriano/análise , Eletroforese em Gel de Campo Pulsado , Concentração de Íons de Hidrogênio , Monoéster Fosfórico Hidrolases/metabolismo , Plantas Medicinais/crescimento & desenvolvimento , Reação em Cadeia da Polimerase , Análise de Componente Principal , RNA Ribossômico 16S/genética , Urease/metabolismo
12.
Oecologia ; 165(4): 847-54, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21190042

RESUMO

Evolution of dispersal is affected by context-specific costs and benefits. One example is sex-biased dispersal in mammals and birds. While many such patterns have been described, the underlying mechanisms are poorly understood. Here, we study genetic and phenotypic traits that affect butterfly flight capacity and examine how these traits are related to dispersal in male and female Glanville fritillary butterflies (Melitaea cinxia). We performed two mark-recapture experiments to examine the associations of individuals' peak flight metabolic rate (MR(peak)) and Pgi genotype with their dispersal in the field. In a third experiment, we studied tethered flight in the laboratory. MR(peak) was negatively correlated with dispersal distance in males but the trend was positive in females, and the interaction between MR(peak) and sex was significant for long-distance dispersal. A similar but nonsignificant trend was found in relation to molecular variation at Pgi, which encodes a glycolytic enzyme: the genotype associated with high MR(peak) tended to be less dispersive in males but more dispersive in females. The same pattern was repeated in the tethered flight experiment: the relationship between MR(peak) and flight duration was positive in females but negative in males. These results suggest that females with high flight capacity are superior in among-population dispersal, which facilitates the spatial spreading of their reproductive effort. In contrast, males with high flight capacity may express territorial behaviour, and thereby increase the number of matings, whereas inferior males may be forced to disperse. Thus, flight capacity has opposite associations with dispersal rate in the two sexes.


Assuntos
Borboletas/metabolismo , Fritillaria/metabolismo , Migração Animal , Animais , Borboletas/genética , Borboletas/crescimento & desenvolvimento , Feminino , Fritillaria/crescimento & desenvolvimento , Genótipo , Glucose-6-Fosfato Isomerase/genética , Glucose-6-Fosfato Isomerase/metabolismo , Masculino , Reprodução , Comportamento Sexual Animal
13.
Zhong Yao Cai ; 33(6): 854-6, 2010 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-21049604

RESUMO

OBJECTIVE: The optimal in vitro culture conditions for the regeneration of Fritillaria cirrhosa were screened. METHODS: Through the in vitro culture of the flowering stage Fritillaria cirrhosa, the effect of illumination, culture temperature, hormone combination and the amount of ribavirin on the regeneration of Fritillaria cirrhosa were studied. RESULTS AND CONCLUSION: The explant browning level could be apparently reduced if the bulb were cultured under the ray after exposure to proper low-temperature. The optimal temperature for the regeneration of bulb is (20 +/- 2) degrees C. The hormone has obvious promotional effect on the regeneration of bulb, and the best combination is 6-BA 2.0 mg/L plus NAA 0.2 mg/L Concentration of 10 mg/L of ribavirin is best for the regeneration of bulb.


Assuntos
Fritillaria/crescimento & desenvolvimento , Fritillaria/metabolismo , Reguladores de Crescimento de Planta/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Ribavirina/metabolismo , Conservação dos Recursos Naturais , Luz , Reguladores de Crescimento de Planta/química , Raízes de Plantas/metabolismo , Regeneração , Ribavirina/química , Temperatura , Técnicas de Cultura de Tecidos/métodos
14.
Zhong Yao Cai ; 33(8): 1201-4, 2010 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-21213528

RESUMO

OBJECTIVE: In order to improving the existent breeds of Fritillaria cirrhosa, increasing its medicinal ingredients and enriching the resources of heredity breeding. METHODS: By using of low-temperature stratification treatment, the seed of Fritillaria cirrhosa completed its after-ripening of physiology and morphology. The induction treatment by different concentrations of colchicine solution and different treatment times for Fritillaria cirrhosa seeds were compared. RESULT: Detected the plant morphology and chromosome number, it is shown that the induced material obviously possessed the characteristics of polyploid. CONCLUSION: With the treatment of 30 mg/L GA3 for 32 h and stratification for 70 d, the seed germination rate of Fritillaria cirrhosa reached 67.0%. After treated with 0.1% colchicine solution for 48 h, the stratificationed mature seed showed polyploid inductivity of 85.7%.


Assuntos
Colchicina/farmacologia , Fritillaria/crescimento & desenvolvimento , Fritillaria/genética , Germinação/efeitos dos fármacos , Poliploidia , Sementes/crescimento & desenvolvimento , Conservação dos Recursos Naturais/métodos , Fritillaria/fisiologia , Giberelinas/farmacologia , Plantas Medicinais/genética , Plantas Medicinais/crescimento & desenvolvimento , Plantas Medicinais/fisiologia , Sementes/efeitos dos fármacos , Sementes/genética , Solventes , Fatores de Tempo
15.
Zhongguo Zhong Yao Za Zhi ; 34(5): 544-6, 2009 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-19526780

RESUMO

OBJECTIVE: To preliminarily study the effect of fertilizer methods on the yield and quality of Fritillaria ussuriensis. METHOD: Through the investigation of the available nitrogen, phosphorus, potassium in soil fertility of F. ussuriensis planting field as well as the measurement of total nitrogen, phosphorus, potassium contents in bulbus of F. ussuriensis, the balanced fertilization plan for cultivation of F. ussuriensis was made. RESULT: The optimal fertilizing amount was 13.0 kg x m(-2) pig dung, or 5.5 kg x m(-2) deer dung and 6.5 kg x m(-2) pig dung, or 11.0 kg x m(-2) deer dung. CONCLUSION: Effective fertilizing may increase significantly the yield and quality of F. ussuriensis.


Assuntos
Agricultura/métodos , Biomassa , Fertilizantes/análise , Fritillaria/crescimento & desenvolvimento , Solo/análise
16.
Zhongguo Zhong Yao Za Zhi ; 33(16): 1948-51, 2008 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-19086625

RESUMO

OBJECTIVE: To study the physiologic indexes, yield and the contents of alkaloids of Fritillaria cirrhosa D. Don under different temperature. METHOD: The growth temperatures (15, 20, 25, 30 degrees C) of F. cirrhosa were controlled by using artificial climate, the growth was observed, the contents of chlorophyll a and b, soluble sugar, MAD, proline of the leaves of F. cirrhosa were tested, and the yield and the alkaloids content of the bulbs were analyzed. RESULT: The growth period of F. cirrhosa under 15, 20 degrees C were appropriately extended. The difference of the content of leaves chlorophyll b under four temperatures and the contents of total chlorophyll and chlorophyll a under 15, 20, 30 degrees C were not significant. The contents of soluble sugar, MAD and proline of leaves and the growth ratio, dry weight and content of alkaloids of bulb increased with the temperature decrease. CONCLUSION: Higher temperature is not suitable for the growth of F. cirrhosa. Under the relatively lower temperature, the growth period of F. cirrhosa extended, the bulb can grow properly, and the content of alkaloid increased. F. cirrhosa can improve its cold tolerance by increasing the content of proline and soluble sugar, and it also can maintain the normal content of chlorophyll under the lower temperature.


Assuntos
Alcaloides/metabolismo , Fritillaria/crescimento & desenvolvimento , Fritillaria/metabolismo , Regulação da Expressão Gênica de Plantas , Temperatura
17.
Zhongguo Zhong Yao Za Zhi ; 33(14): 1645-8, 2008 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-18841756

RESUMO

The paper reviews the recent developments in germplasm, identification, ecological factors and cultivation of bulbus fritillariae cirrhosae. During past years, new methods for identification such as mass spectrometry (MS), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA chips have been invented and identification works became easier. The relationship between ecological factors such as climate, soil, community types and the quality of bulbus fritillariae cirrhosae has been revealed, thus a new cultivation model "semi-wild cultivation" have been developed and it is beneficial to material conservation. The present problems and the future research direction on bulbus fritillariae cirrhosae were also discussed.


Assuntos
Fritillaria/química , Fritillaria/crescimento & desenvolvimento , Conservação dos Recursos Naturais , Medicamentos de Ervas Chinesas/química , Monitoramento Ambiental , Fritillaria/genética
18.
Z Naturforsch C J Biosci ; 63(11-12): 789-93, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19227824

RESUMO

Ten strains of endophytic fungi were isolated from the bulbs of the traditional Chinese medicinal plant Fritillaria ussuriensis. The extract from one of them, Fu7, showed a positive reaction with Dragendorff's reagent and the same Rf value in thin-layer chromatography (TLC) analysis as authentic sipeimine. A further TLC scan and high-performance liquid chromatography-evaporative light-scattering detection (HPLC-ELSD) showed that one ingredient of the extract of strain Fu7 had a similar absorption curve in the range 200-700 nm and the same retention time as authentic sipeimine. Thus, the fungus produces the bioactive ingredient sipeimine, as does its host plant, and could be used for the production of sipeimine by fermentation.


Assuntos
Cevanas/isolamento & purificação , Cevanas/metabolismo , Fritillaria/metabolismo , China , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Delgada , Medicamentos de Ervas Chinesas/isolamento & purificação , Filtração , Fritillaria/química , Fritillaria/crescimento & desenvolvimento , Raízes de Plantas/química
19.
Zhongguo Zhong Yao Za Zhi ; 33(22): 2603-6, 2008 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-19216151

RESUMO

OBJECTIVE: To study the optimal condition for bulb induction of Fritillaria anhuiensis in vitro. METHOD: Effects of sucrose, salicylic acid, active carbon and 5 degrees C pre-incubation on bulb formation in vitro were investigated by adopting the number of bulb and fresh weight as indexes. RESULT: The number of bulb and fresh weight in medium added with 50 g x L(-1) sucrose were significantly higher than those with other treatments. The medium with different concentrations of salicylic acid showed no significant promotion on callus differentiation. However, 5 g x L(-1) active carbon (AC) treatment was better than other treatments. Meanwhile, a large number of plantlets formed after 5 degrees C pre-incubation for 30-40 days was suitable for bulb formation and growth. CONCLUSION: Bulblet formation was optimal in MS + KT 2 mg x L(-1) + NAA 2 mg x L(-1) + 50 g x L(-1) sucrose +5 g x L(-1) AC after callus pre-incucation at 5 degrees 1 for 30-40 days.


Assuntos
Fritillaria/crescimento & desenvolvimento , Caules de Planta/efeitos dos fármacos , Caules de Planta/crescimento & desenvolvimento , Fritillaria/efeitos dos fármacos , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/crescimento & desenvolvimento , Ácido Salicílico/farmacologia , Sacarose/farmacologia , Temperatura
20.
Zhongguo Zhong Yao Za Zhi ; 32(16): 1628-30, 2007 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-18027652

RESUMO

OBJECTIVE: To determine peimine, peiminine and zhebeinine in Fritillaria thunbergii simultaneously by RP-HPLC-ELSD. METHOD: The HPLC was carried out with a Waters Alliance, Model 2690, equipped with an Xterra RP18 column (3.9 mm x 150 mm, .5 microm), and evaporated light scattering detector. It was eluted gradiently with acetonitrile and 10 mmol x L(-1) NH4HCO3 (adjusted to pH 10. 10 by ammonia solution). RESULT: The average recovery rates (n=5) of peimine, peiminine and zhebeinine were 98.2% (RSD 1.7%), 98.2% (RSD 2.2%), 96.5% (RSD 1.3%) respectively. CONCLUSION: The method is simple, sensitive and reliable. It can be used for quantitative determination of F. thunbergii.


Assuntos
Cevanas/análise , Cromatografia Líquida de Alta Pressão/métodos , Fritillaria/química , China , Cromatografia Líquida de Alta Pressão/instrumentação , Ecossistema , Fritillaria/crescimento & desenvolvimento , Luz , Plantas Medicinais/química , Plantas Medicinais/crescimento & desenvolvimento , Reprodutibilidade dos Testes , Espalhamento de Radiação
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