Combining ability analysis in bitter gourd (Momordica charantia L. ) for potential quality improvement / Análise de capacidade de combinação em cabaça-amarga (Momordica charantia L. ) para melhoria potencial da qualidade

Abstract Combining ability analysis provides useful information for the selection of parents, also information regarding the nature and magnitude of involved gene actions. Crops improvement involves strategies for enhancing yield potentiality and quality components. Targeting the improvement of respective characters in bitter gourd, combining ability and genetic parameters for 19 characters were estimated from a 6×6 full diallel analysis technique. The results revealed that the variances due to general combining ability (GCA) and specific combining ability (SCA) were highly significant for most of the important characters. It indicated the importance of both additive and non-additive gene actions. GCA variances were higher in magnitude than SCA variances for all the characters studied indicating the predominance of the additive gene effects in their inheritance. The parent P2 (BG 009) appeared as the best general combiner for earliness; P1 (BG 006) for number of fruits, average single fruit weight and fruit yield; P4 (BG 027) for node number of first female flower and days to seed fruit maturity; P3 (BG 011) for fruit length and thickness of the fruit flesh; P5 (BG 033) for 100-seed weight; and P6 for number of nodes per main vine. The SCA effect as well as reciprocal effect was also significant for most of the important characters in different crosses.

Resumo A análise da capacidade de combinação fornece informações úteis para a seleção dos pais, também informações sobre a natureza e a magnitude das ações dos genes envolvidos. A melhoria das safras envolve estratégias para aumentar a potencialidade da produção e os componentes de qualidade. Visando ao aprimoramento dos respectivos caracteres em cabaça-amarga, capacidade de combinação e parâmetros genéticos para 19 caracteres, foram estimados a partir de uma técnica de análise dialélica completa 6 × 6. Os resultados revelaram que as variâncias, devido à capacidade geral de combinação (GCA) e capacidade específica de combinação (SCA), foram altamente significativas para a maioria dos caracteres importantes. Indicou a importância das ações gênicas aditivas e não aditivas. As variâncias GCA foram maiores em magnitude do que as variâncias SCA para todos os caracteres estudados, indicando a predominância dos efeitos do gene aditivo em sua herança. O pai P2 (BG 009) apareceu como o melhor combinador geral para o início; P1 (BG 006) para número de frutos, peso médio de um único fruto e produção de frutos; P4 (BG 027) para número de nó da primeira flor fêmea e dias para a maturidade do fruto da semente; P3 (BG 011) para comprimento do fruto e espessura da polpa do fruto; P5 (BG 033) para peso de 100 sementes; e P6 para o número de nós por videira principal. O efeito SCA, bem como o efeito recíproco, também foi significativo para a maioria dos personagens importantes em cruzamentos diferentes.

Identification and stability evaluation of polyphenol oxidase substrates of pineapple fruit.

Pineapple as an important tropical fruit in the world is prone to internal browning (IB) caused by the oxidation of phenolic substances. To address the occurrence of IB, endogenous polyphenol oxidase (PPO) substrates were extracted, separated, and purified from pineapple fruit, and then four compounds were obtained and identified as catechin, epicatechin, chlorogenic acid and pyrocatechol. The contents of these substrates increased, reached the maximum value in the middle storage period, decreased gradually, and then maintained a relatively stable level by the end of storage. Meanwhile, pineapple PPO substrates were unstable in structure and susceptible to be degraded under the condition of light, high temperature (>40 °C), alkalinity (pH > 9) or metal ion (Ca2+, Cu2+, Fe2+, Pb2+ and Zn2+). Additionally, the presences of O2 and H2O2 increased the enzymatic oxidation of the pineapple PPO substrates, whereas CO2, N2, Vc (Vitamin C), l-cysteine, Na2SO3 and Na2S2O5 inhibited this oxidation.

A blend of Withania somnifera (L.) Dunal root and Abelmoschus esculentus (L.) Moench fruit extracts relieves constipation and improves bowel function: A proof-of-concept clinical investigation.

ETHNOPHARMACOLOGICAL RELEVANCE: Withania somnifera (L.) Dunal (WS) and Abelmoschus esculentus (L.) Moench (AE) are known as Ashwagandha and Okra, respectively, important herbs in traditional medicine for their diverse therapeutic values. WS root is an adaptogen that relieves stress and anxiety and promotes sleep. AE fruit or Okra is widely consumed as a vegetable and is traditionally used to treat diabetes, gastric irritations, ulcers, and obesity. AIM OF THE STUDY: The present randomized, double-blind, placebo-controlled study aimed to establish a proof-of-concept evaluating the efficacy and tolerability of a proprietary blend of standardized extracts of WS root and AE fruit, CL18100F4 in relieving constipation and improving quality of life in adults. MATERIALS AND METHODS: Forty-eight male and female participants (age: 25-60 years) with functional constipation (following Rome-III criteria) were randomized into placebo, 300 or 500 mg of CL18100F4 groups, and supplemented for fourteen consecutive days. RESULTS: CL18100F4 supplementation significantly (p < 0.0001) reduced the Patient Assessment of Constipation-Symptoms (PAC-SYM), Patient Assessment of Constipation-Quality of Life (PAC-QOL), and Gastrointestinal Symptom Rating Scale (GSRS) scores. CL18100F4 supplementation improved sleep quality and reduced stress (p < 0.0001). At the end of the study, CL18100F4-500 subjects showed significant increases in serum serotonin, gastrin, and interleukin-10 and decrease in interleukin-6 and cortisol levels. Participants' hematology, total blood chemistry, vital signs, and urinalysis parameters were within the normal ranges. No adverse events were reported. CONCLUSIONS: This short-duration, single-site clinical investigation demonstrates that CL18100F4 supplementation is tolerable, helps relieve constipation, reduces stress, and improves gastrointestinal function, sleep quality, and general wellness in adults. TRIAL REGISTRATION: Clinical Trials Registry- India (CTRI/2020/11/029320); Registered on 24/11/2020. Available at: http://ctri.nic.in/Clinicaltrials/showallp.php?mid1=49391&EncHid=&userName=CL18100F4.

Himalayan Pyracantha crenulata (D.Don) M.Roem. leaf and fruit extracts alleviate algesia through COX-2 and Mu-opioid receptor mediated pathways.

ETHNOPHARMACOLOGICAL RELEVANCE: Pyracantha crenulata (D.Don) M.Roem., a plant of high nutritional and medicinal value is traditionally employed for its analgesic property in joint and body pain in the Kumaun region of Western Himalaya. AIM OF THE STUDY: To validate the traditional claims for analgesic property of Pyracantha crenulata. METHODS: Hydroethanolic extract of P. crenulata leaves and fruits were tested for their analgesic potential in rodent models for algesia by tail immersion test, tail flick test, Eddy's hot plate model, and formalin induced paw irritation test in Wistar rats. Molecular docking and dynamics studies were also performed to understand the possible mechanisms. RESULTS: Both P. crenulata fruit extract and leaf extract exhibited significant amelioration in all the tested experimental models of algesia acting through central and peripheral mechanisms. The efficacy in reducing nociception was found comparable to diclofenac that was used as a reference standard. Molecular docking and dynamic simulation studies further established binding affinity of gallic acid (confirmed to be present in P. crenulata leaf extract through HPTLC profiling) with cyclooxygenase-2 (COX-2) and mu-opioid receptors, suggesting the modulatory effect of these extracts on COX-2 and mu-opioid receptors in combating algesia. CONCLUSION: P. crenulata extracts produce analgesic effects plausibly through COX-2 and mu-opioid receptor mediated pathways.

Understanding quality differences between kiwifruit varieties during softening.

Research into variations between kiwifruit varieties particularly their softening quality during storage is important in improving kiwifruit quality. The potential reasons for ripening quality differences between 'Cuixiang' (CX) and 'Hayward' (HWD) kiwifruit were analyzed by physiology and metabolomic data combined with the random forests learning algorithm. The results showed that the storability difference between the two varieties mainly resulted from differences in polygalacturonase (PG) and ß-galactosidase activities. The 1 °C slowed the fruit softening process of both varieties by decreasing their PG activities. A total of 368 metabolites were identified and amino acid, carbohydrate, cofactors and vitamins, as well as nucleotide metabolism are key metabolic modules that affect the ripening differences of CX and HWD kiwifruit. A total of 30 metabolites showed remarkable ability in distinguish the ripening quality of CX and HWD kiwifruit, in which d-glucose, d-maltose, 2-hydroxybutyric acid, phenyllactate, and vitamin B2 were noteworthy for their potential application on the evaluation of kiwifruit taste and nutritional value. These findings provide positive insights into the underlying mechanism of ripening quality differences between CX and HWD kiwifruit and new ideas for identifying key metabolic markers in kiwifruit.

Targeted metabolic profiling of the revived ancient 'Corbella' olive cultivar during early maturation.

'Corbella' is an ancient olive cultivar whose cultivation has recently been revived and hence little is known about its composition. This is the first work studying the metabolic profile of 'Corbella' olives during early maturation. Olives with a ripening index (RI) < 1 yielded considerably less oil content (<40%) but had more concentration of phenolic compounds (148.41-219.70 mg/kg), carotenoids (9.61-14.94 mg/kg) and squalene (521.41-624.40 mg/kg). Contrarily, the levels of α-tocopherol were higher at the RI of 1.08 and 1.96 (64.57 and 57.75 mg/kg, respectively). The most abundant phenolic compound was oleuropein aglycone (>50% of the phenolic composition), suggesting a high hydrolytic activity of ß-glucosidase in the fruit. The antioxidant capacity was barely affected, while oleic/linoleic ratio reached its highest at RI of 1.96. Therefore, olives with an RI below 2 could be good candidates to produce high-quality olive oils with good level of stability.

Potential use of grape and wine polysaccharide extracts as fining agents to modulate the volatile composition of Viura wines.

This paper describes for the first time the use of grape derived polysaccharide extracts as potential fining agents to modulate the volatile composition of Viura white wines. Polysaccharide extracts were obtained from white grape pomace, red wine pomace, white must, red must, white wine, and lees from white wine. Except for higher alcohols, the extracts from white pomace, red pomace and white lees increased the content of most volatile compounds after one and twelve months of bottle aging. They could be used to enhance fruity and floral aromas and reduce unpleasant aromas, showing as good modulators of white wine aroma. The presence of mannoproteins, glucans, non-pectic polysaccharides, and low molecular weight polysaccharides increased the content of most volatile families. Polysaccharides of medium molecular weight showed negative correlations with volatile contents. Our results support the use of winemaking by-products to obtain valuable polysaccharides, contributing to the circular economy.

Integrated ESI-MS/MS and APCI-MS/MS based metabolomics reveal the effects of canning and storage on peach fruits.

The characterization of peach metabolites and carotenoids during canning and storage remains unclear. The present study identified 658 metabolites and 40 carotenoids in peach fruits throughout the canning and storage using ESI-MS/MS and APCI-MS/MS based metabolome approach. A total of 282 differentially accumulated metabolites were found, mainly including 78 phenolic acids, 74 lipids, 61 flavonoids. Five esterified carotenoids (rubixanthin palmitate, ß-cryptoxanthin oleate, ß-cryptoxanthin laurate, ß-cryptoxanthin palmitate, and ß-cryptoxanthin myristate) were the main peach carotenoids, with a proportion of approximately 90%, while free carotenoids accounted for 4.22-5.95% during the entire processing period. Moreover, the total carotenoid loss rates for canning and storage were 56.67% and 46.55%, respectively. Compared to the loss of free carotenoids, esterified carotenoids were more stable during storage, while canning led to a greater loss of esterified carotenoids. The results provided new insights into the maintenance of health-related phytochemicals from canning processes.

Insight into the comparison of key aroma-active compounds between camellia oils from different processing technology.

Currently, the difference between key odorants of camellia oils from different processing technology (i.e., extra virgin camellia oil (EVCO), virgin camellia oil (VCO), fragrant camellia oil (FCO)) is unclear. In this study, a total of 91 odorants were identified by comprehensive two-dimensional gas chromatography and quadrupole mass spectrometry (GC × GC-qMS). The headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) provided fingerprint information for 57 odorants distinguished between EVCO, VCO, and FCO. Moreover, 76 odorants were shown flavor dilution (FD) factors range from 1 to 729, and fruity esters (ethyl 2-hydroxypropanoate, ethyl decanoate, and ethyl phenylacetate) with FD factors ≥ 27 and odor activity values ≥ 1 are the unique odorants in EVCO. (E, E)-2,4-Heptadienal, (E, E)-2,4-nonadienal, and d-limonene are the aroma-active compounds in VCO. While furfural and 3-ethyl-2,5-dimethylpyrazine with FD factors ≥ 243 are the major contributors to roasted and nutty odor in FCO. This work provides aroma markers for quality assessment of camellia oils.

Eco-friendly thermosensitive magnetic-molecularly-imprinted polymer adsorbent in dispersive solid-phase microextraction for gas chromatographic determination of organophosphorus pesticides in fruit samples.

A thermosensitive magnetic-molecularly-imprinted polymer (TMMIP) was successfully prepared in an aqueous medium. The TMMIP was applied as an effective adsorbent in dispersive solid-phase microextraction for the selective enrichment of five organophosphorus pesticides (OPPs; diazinon, fenitrothion, fenthion, parathion-ethyl, and ethion) before analysis by gas chromatography. The polymerization was performed using mixed-valence iron hydroxide nanoparticles as the magnetic support, N-isopropyl acrylamide as the thermosensitive monomer, ethion as the template, and methacrylic acid as the functional monomer. The adsorption and desorption mechanisms of OPPs depend on their interactions with the adsorbents and solution temperature. Our methodology provides good linearity (0.50-2000 µgL-1), with a correlation determination of R2 > 0.9980, low limit of detection (0.25-0.50 µgL-1), low limit of quantitation (0.50-1.50 µg L-1), and high precision (%RSD < 7%). The developed method demonstrates excellent applicability for accurately and efficiently determining OPP residuals in fruit and vegetable samples with good recoveries (93-117%).

Effects of phytase/ethanol treatment on aroma characteristics of rapeseed protein isolates.

This study investigates enhancing the flavor of rapeseed protein isolate (RPI), a protein-rich substance with an unfavorable taste, through phytase/ethanol treatment. Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOF-MS) analysis identified 268 volatile compounds in RPI. The study found that this treatment significantly altered the content of these compounds, reducing sourness and enhancing sweetness and fruitiness. The analysis also showed that the treatment notably increased the relative odor activity values (ROAVs) of key aroma compounds, improving RPI's flavor. Sensory evaluation confirmed the positive impact of the treatment, indicating its potential to make RPI a more acceptable ingredient in the food industry.

Intervention mechanisms of cold plasma pretreatment on the quality, antioxidants and reactive oxygen metabolism of fresh wolfberries during storage.

Fresh wolfberries, a nutritious "super fruit", face limited marketing potential due to storage difficulties. This study aimed to enhance their storage stability using dielectric barrier discharge plasma (DBD) pretreatment and investigate the intervention mechanism. The results indicated that the optimal condition of DBD pretreatment for fresh wolfberries was 13.64 kV, 70 s and 2.7 kHz, which extended their shelf from 2 to 5 d at room temperature. This pretreatment reduced decay, weight loss, and firmness reduction by inactivating microorganisms and inhibiting respiration. Additionally, the decline of phenols, flavonoids, ascorbic acid, and antioxidant activity was inhibited, while maintaining high content of polysaccharides, titratable acid, and carotenoids. Interestingly, moderate DBD treatment produced reactive oxygen species (ROS) that triggered the defense response of wolfberries' ROS metabolism system and promoted the biosynthesis of flavonoids, thereby enhancing resistance to decay. The findings offer new insight into plasma effects on fruits and vegetables.

Effects of extraction methods on the structure and functional properties of soluble dietary fiber from blue honeysuckle (Lonicera caerulea L.) berry.

The work within this study aimed to investigate and compare the effects of compound enzyme extraction (CE), ultrasonic chemical extraction (UC) and combined fermentation extraction (CF) on the physicochemical properties, microstructure, and functional properties of soluble dietary fiber (SDF) extracted from blue honeysuckle berries. The results showed that CE-SDF had higher crystallinity (32.41%). UC-SDF had the highest yield (13.32 ± 0.80 g/100 g). CF-SDF had the maximum inhibition of α-amylase (50.82 ± 0.76%) and α-glucosidase (54.87 ± 1.25%). The in vitro hypoglycemic activity of the three SDFs was observed in the order of CF > CE > UC. Meanwhile, the purity of SDF had a strong positive correlation with its antioxidant and in vitro hypoglycemic capacities. The crystallinity of SDF was found to be positively correlated with its molecular weight and thermal properties. Additionally, the sugar composition of SDF was found to be an important factor affecting its biological activity.

Ultrasensitive two-photon probes for rapid detection of ß-galactosidase during fruit softening and cellular senescence.

Senescence is happening in every corner of the living organisms. ß-galactosidase (ß-gal) is one of the most important biomarkers during senescence in both plant and mammalian cells. Most ß-gal fluorescent probes were focused on bio-imaging, only a few probes were developed for the detection of ß-gal in fruit, and the probes that could detect ß-gal in both fruits and living cells were even less. Here, two ß-gal probes (TNap-ßGal and TBNap-ßGal) were synthesized, which can not only image the increase of ß-gal during both fruits softening and cellular senescence, but also prove that bananas are not suitable for storage in refrigerator and the subsequent accumulation of ß-gal still in lysosome of mammalian cells. In addition, TNap-ßGal was successfully applied to two-photon imaging of endogenous ß-gal in both hDPMSCs and tissues of human dental pulp for the first time.

Phytochemical and functional characterization of cultivated varieties of Morus alba L. fruits grown in Italy.

Morus alba L. fruits are considered functional foods with an important nutritional value for their high content of polyphenols. Therefore, the type and level of phytochemicals of the soroses from 13 M. alba cultivars grown in Italy were characterized due to the lack of data available about their nutraceutical properties. Mature M. alba fruits exhibited variable polyphenol, flavonoid, anthocyanin, proanthocyanins, and 1-deoxynojirimycin contents which resulted in different antioxidant and α-glucosidase inhibitory activities. Regression models built on UHPLC-HRMS results revealed a strong correlation between the expression of quercetin derivatives, cyanidin 3-O-glucoside, caffeoyl methyl quinates, and 5,5'-dehydrodivanillic acid, and the biological activity of each fruit. On another note, principal component analysis revealed that the quantity of caffeoyl/dicaffeoyl methyl quinate, caffeoylquinic acids, and quercetin derivatives decreased during ripening. The results on the compositional and functional characterization of mature M. alba fruits might improve their consumption and economic value in Italy.

(E)-2-hexenal fumigation control the gray mold on fruits via consuming glutathione of Botrytis cinerea.

(E)-2-hexenal fumigation inhibits the growth of Botrytis. cinerea, but the direct target and its effect on postharvest strawberry have not yet been discovered. In the present study, we applied increasing level of (E)-2-hexenal fumigation from 0.524 µM to 1048 µM to B. cinerea on strawberry and medium. Results showed that (E)-2-hexenal fumigation inhibited lesion diameter on strawberry by 13.96 %, 20.41 % and 100 % with the dosage increasing. On medium, (E)-2-hexenal fumigation increased both the ROS and mitochondria membrane potential level of B. cinerea. LC-MS/MS and FTIR results demonstrate a 1:1 Michael addition reaction between (E)-2-hexenal and glutathione with the product GSH-H in B. cinerea under (E)-2-hexenal fumigation. Furthermore, the consumption of glutathione and glutathione disulfide along with the production of GSH-H during fumigation in B. cinerea caused by (E)-2-hexenal were both concentration- and time-dependent. This study locates the direct target and discovered the functional model of (E)-2-hexenal to B. cinerea.

An analytical approach to determine the health benefits and health risks of consuming berry juices.

Food products composition analysis is a prerequisite for verification of product quality, fulfillment of regulatory enforcements, checking compliance with national and international food standards, contracting specifications, and nutrient labeling requirements and providing quality assurance for use of the product for the supplementation of other foods. These aspects also apply to the berry fruit and berry juice. It also must be noted that even though fruit juices are generally considered healthy, there are many risks associated with mishandling both fruits and juices themselves. The review gathers information related with the health benefits and risk associated with the consumption of berry fruit juices. Moreover, the focus was paid to the quality assurance of berry fruit juice. Thus, the analytical methods used for determination of compounds influencing the sensory and nutritional characteristics of fruit juice as well as potential contaminants or adulterations.

Mechanisms of persimmon pectin methyl esterase activation by high pressure processing based on chemical experiments and molecular dynamics simulations.

High pressure processing (HPP) was found to have a kinase effect on persimmon pectin methyl esterase (PME), while the mechanism remains unclear. In this study, chemical experiments and molecular dynamics (MD) simulations were used to reveal its mechanisms. Persimmon PME was first extracted and purified using ion exchange columns with 81.89% purity. After 500 MPa/5 min, PME activity increased 11.3%, the α-helix and ß-folding decreased 10.8% and 6.1% compared to the 0.1 MPa group, respectively. MD results showed that HPP decreased the volume, increased the number of hydrogen bonds between PME and pectin. Under high pressure, Asp-157, Asp-136 and Gln-135 in the enzyme activity center remained stable, while the positions of Arg-225 and Gln-113 changed a lot. The conformation of the substrate binding channel also changed. The secondary structure and volume changes of the HPP-treated PME affected the active center and substrate channels, ultimately altering the activity.

Guabijú (Myrcianthes pungens): A comprehensive evaluation of anthocyanins and free, esterified, glycosylated, and insoluble phenolic compounds in its peel, pulp, and seeds.

Guabijú (Myrcianthes pungens) is a Brazilian native fruit from the Myrtaceae family, with few studies on the presence of phenolic compounds. Free, esterified, glycosylated, and insoluble phenolic compounds were studied for the first time in guabijú peel, pulp, and seed, by liquid chromatography coupled to mass spectrometry (LC-ESI-QTOF-MS/MS and LC-ESI-QqQ-MS/MS). Eighty-one phenolic compounds were tentatively identified in the three fractions, and eighteen were quantified using authentic standards. Furthermore, six anthocyanins were quantified in guabijú peel. Among the tentatively identified phenolic compounds, most belonged to the flavonols class. Major compounds quantified in the different fractions were ellagic and gallic acids, mainly in the hydrolyzed fractions. The peel presented the highest contents for most phenolic compounds, followed by the seed and pulp. This new data will add value to the fruit and facilitate the development of new products, as well as favoring and stimulating the consumption of the fruit.