Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 384
Filtrar
1.
Plant Mol Biol ; 101(1-2): 149-162, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31267255

RESUMO

KEY MESSAGE: Here we describe that the regulation of MdWRKY31 on MdHIR4 in transcription and translation levels associated with disease in apple. The phytohormone salicylic acid (SA) is a main factor in apple (Malus domestica) production due to its function in disease resistance. WRKY transcription factors play a vital role in response to stress. An RNA-seq analysis was conducted with 'Royal Gala' seedlings treated with SA to identify the WRKY regulatory mechanism of disease resistance in apple. The analysis indicated that MdWRKY31 was induced. A quantitative real-time polymerase chain reaction (qPCR) analysis demonstrated that the expression of MdWRKY31 was induced by SA and flg22. Ectopic expression of MdWRKY31 in Arabidopsis and Nicotiana benthamiana increased the resistance to flg22 and Pseudomonas syringae tomato (Pst DC3000). A yeast two-hybrid screen was conducted to further analyze the function of MdWRKY31. As a result, hypersensitive-induced reaction (HIR) protein MdHIR4 interacted with MdWRKY31. Biomolecular fluorescence complementation, yeast two-hybrid, and pull-down assays demonstrated the interaction. In our previous study, MdHIR4 conferred decreased resistance to Botryosphaeria dothidea (B. dothidea). A viral vector-based transformation assay indicated that MdWRKY31 evaluated the transcription of SA-related genes, including MdPR1, MdPR5, and MdNPR1 in an MdHIR4-dependent way. A GUS analysis demonstrated that the w-box, particularly w-box2, of the MdHIR4 promoter played a major role in the responses to SA and B. dothidea. Electrophoretic mobility shift assays, yeast one-hybrid assay, and chromatin immunoprecipitation-qPCR demonstrated that MdWRKY31 directly bound to the w-box2 motif in the MdHIR4 promoter. GUS staining activity and a protein intensity analysis further showed that MdWRKY31 repressed MdHIR4 expression. Taken together, our findings reveal that MdWRKY31 regulated plant resistance to B. dothidea through the SA signaling pathway by interacting with MdHIR4.


Assuntos
Resistência à Doença , Malus/genética , Doenças das Plantas/imunologia , Reguladores de Crescimento de Planta/farmacologia , Proteínas de Plantas/metabolismo , Ácido Salicílico/farmacologia , Arabidopsis/genética , Arabidopsis/imunologia , Arabidopsis/microbiologia , Ascomicetos/fisiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Frutas/genética , Frutas/imunologia , Frutas/microbiologia , Regulação da Expressão Gênica de Plantas , Genes Reporter , Malus/imunologia , Malus/microbiologia , Doenças das Plantas/microbiologia , Proteínas de Plantas/genética , Regiões Promotoras Genéticas/genética , Pseudomonas syringae/fisiologia , Plântula/genética , Plântula/imunologia , Plântula/microbiologia , Transdução de Sinais , Tabaco/genética , Tabaco/imunologia , Tabaco/microbiologia , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Técnicas do Sistema de Duplo-Híbrido
2.
Phytochemistry ; 165: 112050, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31252202

RESUMO

In higher plants, asparagine-linked oligosaccharides (N-glycans) in glycoproteins carry unique carbohydrate epitopes, namely, a core α1,3-fucose and/or a ß1,2-xylose, which are common determinants responsible for the cross-reactivity of plant glycoproteins due to their strong immunogenicity. While these determinants and the relevant genes have been well characterized for herbaceous plants, information concerning whether many food plants cross-react with airborne pollens is not available. In this paper, we report on the characterization of a novel core α1,3-fucosyltransferase gene identified from Mangifera indica L., one of the major plants potentially related to food allergy. Based on sequence information of plant homologues, we amplified a candidate cDNA (MiFUT11) from pericarp tissue. An in vitro assay demonstrated that the recombinant MiFUT11 protein transfers a fucose unit onto both non-fucosylated and core α1,6-fucosylated oligosaccharides. A glycoform analysis using MALDI-TOF mass spectrometry showed that the introduction of the MiFUT11 cDNA increased the production of a core α1,3- and α1,6-fucosylated pauci-mannosidic oligosaccharide in Spodoptera Sf21 cells. Our findings suggest that MiFUT11 is a functional core α1,3-fucosyltransferase gene that is involved in the assembly of cross-reactive N-glycans in mango fruit.


Assuntos
Carboidratos/biossíntese , Frutas/química , Fucosiltransferases/metabolismo , Mangifera/enzimologia , Sequência de Aminoácidos , Carboidratos/genética , Carboidratos/imunologia , Frutas/imunologia , Frutas/metabolismo , Fucosiltransferases/química , Fucosiltransferases/genética , Mangifera/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência
3.
J Agric Food Chem ; 67(22): 6116-6124, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-31084000

RESUMO

Melatonin acts as a crucial signaling molecule with multiple physiological functions in plant response to abiotic and biotic stresses. However, the impact and regulatory mechanism of melatonin on attenuating tomato fruit fungal decay are unclear. In this study, we investigated the potential roles of melatonin in modulating fruit resistance to Botrytis cinerea and explored related physiological and molecular mechanisms. The results revealed that disease resistance was strongly enhanced by melatonin treatment, and 50 µM was confirmed as the best concentration. Melatonin treatment increased the activities of defense-related enzymes and decreased hydrogen peroxide (H2O2) content with enhanced antioxidant enzyme activities. Moreover, we found that melatonin treatment increased methyl jasmonate (MeJA) content; up-regulated the expressions of SlLoxD, SlAOC, and SlPI II; and reduced the expressions of SlMYC2 and SlJAZ1. We postulated that melatonin played a positive role in tomato fruit resistance to Botrytis cinerea through regulating H2O2 level and JA signaling pathway.


Assuntos
Botrytis/fisiologia , Ciclopentanos/imunologia , Lycopersicon esculentum/efeitos dos fármacos , Lycopersicon esculentum/microbiologia , Melatonina/farmacologia , Oxilipinas/imunologia , Doenças das Plantas/imunologia , Frutas/efeitos dos fármacos , Frutas/genética , Frutas/imunologia , Frutas/microbiologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Peróxido de Hidrogênio/imunologia , Lycopersicon esculentum/genética , Lycopersicon esculentum/imunologia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Reguladores de Crescimento de Planta/imunologia , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Transdução de Sinais/efeitos dos fármacos
4.
J Agric Food Chem ; 67(24): 6725-6735, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31117506

RESUMO

Jasmonic acid (JA)- and ethylene-mediated signaling pathways are reported to have synergistic effects on inhibiting gray mold. The present study aimed to explain the role of ethylene perception in methyl jasmonate (MeJA)-mediated immune responses. Results showed that exogenous MeJA enhanced disease resistance, accompanied by the induction of endogenous JA biosynthesis and ethylene production, which led to the activation of the phenolic metabolism pathway. Blocking ethylene perception using 1-methylcyclopropene (1-MCP) either before or after MeJA treatment could differently weaken the disease responses induced by MeJA, including suppressing the induction of ethylene production and JA contents and reducing activities of lipoxygenase and allene oxide synthase compared to MeJA treatment alone. Consequently, MeJA-induced elevations in the total phenolic content and the activities of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase, 4-coumarate:coenzyme A ligase, and peroxidase were impaired by 1-MCP. These results suggested that ethylene perception participated in MeJA-mediated immune responses in tomato fruit.


Assuntos
Acetatos/imunologia , Botrytis/fisiologia , Ciclopentanos/imunologia , Etilenos/imunologia , Lycopersicon esculentum/imunologia , Oxilipinas/imunologia , Doenças das Plantas/imunologia , Reguladores de Crescimento de Planta/imunologia , Resistência à Doença , Frutas/imunologia , Frutas/microbiologia , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/genética , Lycopersicon esculentum/microbiologia , Fenilalanina Amônia-Liase/genética , Fenilalanina Amônia-Liase/imunologia , Doenças das Plantas/microbiologia , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Transcinamato 4-Mono-Oxigenase/genética , Transcinamato 4-Mono-Oxigenase/imunologia
5.
J Agric Food Chem ; 67(12): 3293-3304, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30785743

RESUMO

The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously high expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack. Pectin induced the antagonistic yeast to secrete pectinolytic enzymes to increase fruit resistance against gray mold. The activities of pathogenic pectinase of B. cinerea were correspondingly depressed in the pectin-inducible yeast enzyme elicited ripe fruit. These data suggest that pectinase is a molecular target for regulation of disease resistance during fruit ripening.


Assuntos
Antibiose , Botrytis/enzimologia , Proteínas Fúngicas/metabolismo , Lycopersicon esculentum/imunologia , Proteínas de Plantas/imunologia , Poligalacturonase/metabolismo , Leveduras/fisiologia , Botrytis/fisiologia , Resistência à Doença , Frutas/crescimento & desenvolvimento , Frutas/imunologia , Frutas/microbiologia , Proteínas Fúngicas/antagonistas & inibidores , Proteínas Fúngicas/genética , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/crescimento & desenvolvimento , Lycopersicon esculentum/microbiologia , Pectinas/imunologia , Proteínas de Plantas/genética , Poligalacturonase/antagonistas & inibidores , Poligalacturonase/genética
6.
J Agric Food Chem ; 67(10): 2801-2810, 2019 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-30794401

RESUMO

The esterified fraction of jujube ( Ziziphus jujuba Mill.) peel extract showed strong antifungal activity on Alternaria alternata. p-Coumaric acid (pCA) was found to be the most predominant individual phenolic acid that was correlated highly with the antifungal activity of the esterified fraction. Thus, effects of postharvest treatments with pCA and its simplest esterified derivative methyl p-coumarate (MeCA) against black spot rot on jujube fruit caused by A. alternata were investigated. pCA and MeCA strongly suppressed in vitro growth of the fungus and significantly reduced postharvest Alternaria rot on fresh jujubes. Biochemical and transcriptional analysis revealed that pCA and MeCA regulated the expression of some genes encoding antioxidant enzymes and their enzymatic activities, enhanced the phenylpropanoid pathway metabolism, and activated the expression of genes encoding pathogenesis-related proteins. These results suggested that, apart from its direct antifungal activity, pCA and MeCA induced defense responses in jujube fruit against postharvest Alternaria rot.


Assuntos
Alternaria/fisiologia , Cinamatos/imunologia , Ácidos Cumáricos/imunologia , Frutas/química , Frutas/imunologia , Doenças das Plantas/microbiologia , Ziziphus/microbiologia , Alternaria/efeitos dos fármacos , Alternaria/crescimento & desenvolvimento , Cinamatos/análise , Ácidos Cumáricos/análise , Frutas/genética , Frutas/microbiologia , Doenças das Plantas/imunologia , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Ziziphus/química , Ziziphus/genética , Ziziphus/imunologia
7.
Allergol. immunopatol ; 47(1): 12-15, ene.-feb. 2019. graf
Artigo em Inglês | IBECS | ID: ibc-180765

RESUMO

Introduction and objectives: Profilin is a panallergen contained in pollen, plant foods and latex. Although cross-reactivity is expected while performing skin prick tests (SPT) with allergens that contain profilin, this is not always noticed. The purpose of this study was to detect if profilin is contained in the commercial SPT extracts of pollen and plant foods which, in their fresh form, contain determined epitopes of profilin. Material and methods: Commercial SPT extracts of different pharmaceuticals were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The study included purified palm date profilin, peach (whole, pulp and peel extracts), hazelnut, Olea europea, Parietaria judaica and Phleum pratense. Results: Profilin was detected in all, but peach extracts; it was neither contained in the whole peach extract nor in the ones of peel or pulp. Conclusion: The only accurate way to detect sensitization to profilin, while performing SPT, is the use of purified profilin extract. Even if a plant food or pollen contain an identified molecule of profilin, the relevant SPT commercial extract may not


No disponible


Assuntos
Humanos , Alérgenos/metabolismo , Antígenos de Plantas/metabolismo , Hipersensibilidade/diagnóstico , Extratos Vegetais/metabolismo , Erros de Diagnóstico/prevenção & controle , Profilinas/metabolismo , Testes Cutâneos/métodos , Alérgenos/imunologia , Antígenos de Plantas/imunologia , Reações Cruzadas , Frutas/imunologia , Olea/imunologia , Parietaria/imunologia , Extratos Vegetais/imunologia , Pólen/imunologia , Profilinas/imunologia , Prunus persica/imunologia
8.
J Agric Food Chem ; 66(38): 9923-9932, 2018 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-30192535

RESUMO

Methyl jasmonate (MeJA) and ethylene play important roles in mediating defense responses against Botrytis cinerea. Ethylene response factors (ERFs) are the final components of ethylene signal transduction; whether SlERF2 participates in disease resistance against Botrytis cinerea is unclear. The objective of this study was to investigate the role of SlERF2 in MeJA-mediated defense response by using both sense and antisense SlERF2 tomato fruit. Our results showed that both MeJA treatment and pathogen infection upregulated SlERF2 expression level. Overexpression of SlERF2 enhanced tomato fruit resistance against Botrytis cinerea. MeJA treatment increased ethylene production, promoted the activities of chitinase, ß-1,3-glucanase, phenylalanine ammonia-lyase, and peroxidase, and elevated pathogenesis-related protein content and total phenolic content. Moreover, the effects of MeJA on disease response were reinforced in sense SlERF2 tomato fruit, while they were weakened in antisense SlERF2 tomato fruit. These results indicated that SlERF2 was involved in MeJA-mediated disease resistance against Botrytis cinerea in tomato fruit.


Assuntos
Acetatos/farmacologia , Botrytis/fisiologia , Ciclopentanos/farmacologia , Lycopersicon esculentum/imunologia , Oxilipinas/farmacologia , Doenças das Plantas/microbiologia , Reguladores de Crescimento de Planta/farmacologia , Proteínas de Plantas/imunologia , Fatores de Transcrição/imunologia , Resistência à Doença , Frutas/efeitos dos fármacos , Frutas/imunologia , Frutas/microbiologia , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/efeitos dos fármacos , Lycopersicon esculentum/microbiologia , Doenças das Plantas/imunologia , Proteínas de Plantas/genética , Fatores de Transcrição/genética
9.
Nutrients ; 10(7)2018 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-30004458

RESUMO

Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria × ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 µg/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 °C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and seasonal effects were minor.


Assuntos
Antígenos de Plantas/imunologia , Betula/imunologia , Hipersensibilidade Alimentar/imunologia , Fragaria/imunologia , Pólen/imunologia , Rinite Alérgica Sazonal/imunologia , Antígenos de Plantas/genética , Antígenos de Plantas/metabolismo , Temperatura Baixa , Produção Agrícola , Reações Cruzadas , Dessecação , Ensaio de Imunoadsorção Enzimática , Manipulação de Alimentos/métodos , Fragaria/genética , Fragaria/crescimento & desenvolvimento , Fragaria/metabolismo , Liofilização , Frutas/imunologia , Genótipo , Temperatura Alta , Humanos , Estabilidade Proteica , Estações do Ano
10.
J Agric Food Chem ; 66(16): 4051-4061, 2018 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-29634265

RESUMO

Freshly consumed peaches ( Prunus persica L. Batsch) can cause allergic reactions in the worldwide population because of the presence of four classes of allergens (Pru p 1, Pru p 2, Pru p 3, and Pru p 4). Fruit bagging has been widely practiced in peach cultivation to improve fruit quality; however, its effect on the expression of peach allergen-encoding genes remains unknown. In this study, the influence of fruit bagging with opaque paper bags on the major peach allergen-encoding genes, including Pru p 1.01, Pru p 1.06B, Pru p 2.01B, Pru p 2.02, Pru p 3.01, Pru p 4.01, and Pru p 4.02, were measured by means of real-time PCR. A significant reduction in transcript accumulation was observed for all of the selected genes in the epicarps of the bagged peach fruits, whereas slight increases were observed in the mesocarps for these genes, with the two exceptions of Pru p 2.02 and Pru p 3.01. For most of these genes, much higher transcripts were determined in the epicarps than in the mesocarps. Taken together, a significant reduction in the transcription rate of the allergen-encoding genes in the whole peach fruit was achieved by shading with opaque paper bags. According to these data, modifications in growing practices of peach may help to obtain fruits with lower levels of allergens and thus contribute to reducing potential allergenic risks in consumers.


Assuntos
Antígenos de Plantas/genética , Produção Agrícola/métodos , Proteínas de Plantas/genética , Prunus persica/imunologia , Antígenos de Plantas/imunologia , Frutas/genética , Frutas/crescimento & desenvolvimento , Frutas/imunologia , Proteínas de Plantas/imunologia , Prunus persica/genética , Prunus persica/crescimento & desenvolvimento
11.
J Sci Food Agric ; 98(13): 5082-5088, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29604076

RESUMO

BACKGROUND: Blue mould caused by Penicillium expansum comprises a notable disease of apple fruit during storage. ϵ-Poly-l-lysine (PL) consists of ϵ-amino and α-hydroxyl and has been used in food preservation. In the present study, apple fruits (cv. Fuji) were used to investigate the effects of PL dipping treatment, at different concentrations of PL, on the lesion diameter of fruit inoculated with P. expansum, aiming to screen the optimal concentration for controlling blue mould. The effects of PL at the optimal concentration on reactive oxygen species (ROS) metabolism and the phenylpropanoid pathway were also investigated. RESULTS: The results indicated that 25, 50, 100 and 200 µL L-1 PL treatment significantly decreased the lesion diameter in apple fruit inoculated with P. expansum and the smallest lesion diameter was determined for 50 µL L-1 PL-treated fruits. The results also indicated that 50 µL L-1 PL treatment increased the hydrogen peroxide content and the activities of enzymes involved in ROS metabolism, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and peroxidase in apple fruit. The activity of phenylalanine ammonia-lyase and the contents of lignin, total phenolic compounds and flavonoids were also enhanced by PL treatment. CONCLUSION: The disease resistance to P. expansum in apple fruits enhanced by PL treatment is related to activating ROS metabolism and the phenylpropanoid pathway and the accumulation of antifungal compounds. © 2018 Society of Chemical Industry.


Assuntos
Resistência à Doença , Malus/imunologia , Malus/microbiologia , Penicillium/fisiologia , Fenilpropionatos/imunologia , Doenças das Plantas/microbiologia , Polilisina/farmacologia , Espécies Reativas de Oxigênio/imunologia , Frutas/imunologia , Frutas/microbiologia , Malus/efeitos dos fármacos , Doenças das Plantas/imunologia
12.
Planta ; 247(6): 1423-1438, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29536219

RESUMO

MAIN CONCLUSION: Latexes in immature fruit, young petioles and lignified trunks of fig trees protect the plant using toxic proteins and metabolites in various organ-dependent ways. Latexes from plants contain high amounts of toxic proteins and metabolites, which attack microbes and herbivores after exudation at pest-induced wound sites. The protein and metabolite constituents of latexes are highly variable, depending on the plant species and organ. To determine the diversity of latex-based defense strategies in fig tree (Ficus carica) organs, we conducted comparative proteomic, transcriptomic and metabolomic analyses on latexes isolated from immature fruit, young petioles and lignified trunks of F. carica after constructing a unigene sequence library using RNA-seq data. Trypsin inhibitors were the most abundant proteins in petiole latex, while cysteine proteases ("ficins") were the most abundant in immature fruit and trunk latexes. Galloylglycerol, a possible defense-related metabolite, appeared to be highly accumulated in all three latexes. The expression levels of pathogenesis-related proteins were highest in the latex of trunk, suggesting that this latex had adapted a defensive role against microbe attacks. Although young petioles and immature fruit are both unlignified soft organs, and potential food for herbivorous insects, unigenes for the sesquiterpenoid pathway, which likely produces defense-associated volatiles, and the phenylpropanoid pathway, which produces toxic furanocoumarins, were expressed less in immature fruit latex. This difference may indicate that while petioles and fruit protect the plant from attack by herbivores, the fruit must also attract insect pollinators at younger stages and animals after ripening. We also suggest possible candidate transcription factors and signal transduction proteins that are involved in the differential expression of the unigenes.


Assuntos
Ficus/imunologia , Perfilação da Expressão Gênica , Látex/metabolismo , Metabolômica , Proteômica , Animais , Ficus/genética , Ficus/metabolismo , Frutas/química , Frutas/genética , Frutas/imunologia , Frutas/metabolismo , Herbivoria , Insetos/fisiologia , Especificidade de Órgãos , Caules de Planta/química , Caules de Planta/genética , Caules de Planta/imunologia , Caules de Planta/metabolismo , Árvores
13.
J Agric Food Chem ; 66(8): 1964-1973, 2018 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-29420890

RESUMO

The inherent allergens of citrus fruits, such as Cit s 1, Cit s 2, Cit s 3 can cause allergic reactions. A better understanding of the genetic factors (cultivar to cultivar) affecting the allergenic potential of citrus fruits would be beneficial for further identification of hypoallergenic genotypes. In the present study, an immunoblotting quantification approach was adopted to assess the potential allergenicity of 21 citrus cultivars, including nine subgroups (tangerine, satsuma, orange, pummelo, grapefruit, lemon, kumquat, tangor, and tangelo). To prepare highly sensitive and specific rabbit polyclonal antibodies, antigenicity of purified rCit s 1.01, rCit s 2.01, and rCit s 3.01 peptides were enhanced with high epitope density in a single protein molecule. The data integration of three citrus allergen quantifications demonstrated that the four pummelo cultivars (Kao Phuang Pummelo, Wanbai Pummelo, Shatian Pummelo, and Guanxi Pummelo) were potential hypoallergenic, compared with other 8 subgroups. Moreover, the immunological analyses with sera of allergic subjects revealed that Shatian Pummelo and Guanxi Pummelo showed the lowest immunoreactivity in 8 representative citrus cultivars. These potential hypoallergenic genotypes are of great significance to not only allergic consumers but also citrus breeders in the genetic improvement of hypoallergenic citrus as breeding resources.


Assuntos
Alérgenos/química , Citrus/química , Immunoblotting/métodos , Proteínas de Plantas/química , Alérgenos/genética , Alérgenos/imunologia , Citrus/classificação , Citrus/genética , Citrus/imunologia , Epitopos/química , Epitopos/genética , Epitopos/imunologia , Frutas/química , Frutas/classificação , Frutas/genética , Frutas/imunologia , Genótipo , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia
14.
Curr Issues Mol Biol ; 27: 89-104, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-28885176

RESUMO

Bioinformatics encompasses many tools and techniques that today are essential for all areas of research in the biological sciences. New databases with a wealth of information about genomes, proteins, metabolites, and metabolic pathways appear almost daily. Particularly, for scientists who carry out research in plant biology, the amount of information has multiplied exponentially due to the large number of databases available for many individual plant species. In this sense, bioinformatics together with next generation sequencing and 'omics' approaches, can provide tools for plant breeding and the genetic engineering of plants. In addition, these technologies enable a better understanding of the processes and mechanisms that can lead to plants with increased tolerance to different abiotic stress conditions and resistance to pathogen attack, as well as the development of crop varieties with improved nutritional quality of seeds and fruits.


Assuntos
Biotecnologia/métodos , Biologia Computacional/métodos , Produtos Agrícolas/genética , Genoma de Planta , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Adaptação Fisiológica , Arabidopsis/genética , Biologia Computacional/instrumentação , Produtos Agrícolas/imunologia , Bases de Dados Genéticas/provisão & distribução , Frutas/genética , Frutas/imunologia , Engenharia Genética/métodos , Sequenciamento de Nucleotídeos em Larga Escala/instrumentação , Melhoramento Vegetal , Imunidade Vegetal/genética , Plantas Geneticamente Modificadas , Sementes/genética , Sementes/imunologia , Estresse Fisiológico
15.
J Sci Food Agric ; 98(8): 3129-3139, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29210450

RESUMO

BACKGROUND: Food traceability becomes lifesaving for persons suffering severe allergy or intolerance, and therefore need a complete avoidance of the immune-trigger food. This paper describes how to fingerprint the presence of some allergenic species (kiwi, peach, and apple) in foods by quantitative real-time PCR (qPCR). RESULTS: Five DNA extraction procedures were tested on fruits and foods. The results were statistically evaluated, and discussed. Analysis by qPCR with SYBR Green was developed to detect traces of these allergenic species in foods. Plasmids containing the target sequences of kiwi, peach and apple were employed as internal reference standard. Analysis of spiked food samples showed a limit of detection of 25 mg kg-1 for kiwi, 20 mg kg-1 for peach and 50 mg kg-1 for apple. CONCLUSION: The qPCR method here developed, combined with the use of internal plasmid reference standard, represents a specific system for the quick detection of allergenic species in complex food matrices, with a limit of detection comparable with those reported using more time-consuming methods. © 2017 Society of Chemical Industry.


Assuntos
Fast Foods/análise , Contaminação de Alimentos/análise , Malus/genética , Prunus persica/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Actinidia/imunologia , Frutas/genética , Frutas/imunologia , Malus/imunologia , Proteínas de Plantas/genética , Proteínas de Plantas/imunologia , Prunus persica/imunologia
16.
Plant J ; 93(2): 270-285, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29160608

RESUMO

Fruits stored at low temperature can exhibit different types of chilling injury. In apple, one of the most serious physiological disorders is superficial scald, which is characterized by discoloration and brown necrotic patches on the fruit exocarp. Although this phenomenon is widely ascribed to the oxidation of α-farnesene, its physiology is not yet fully understood. To elucidate the mechanism of superficial scald development and possible means of prevention, we performed an integrated metabolite screen, including an analysis of volatiles, phenols and lipids, together with a large-scale transcriptome study. We also determined that prevention of superficial scald, through the use of an ethylene action inhibitor, is associated with the triggering of cold acclimation-related processes. Specifically, the inhibition of ethylene perception stimulated the production of antioxidant compounds to scavenge reactive oxygen species, the synthesis of fatty acids to stabilize plastid and vacuole membranes against cold temperature, and the accumulation of the sorbitol, which can act as a cryoprotectant. The pattern of sorbitol accumulation was consistent with the expression profile of a sorbitol 6-phosphate dehydrogenase, MdS6PDH, the overexpression of which in transgenic Arabidopsis thaliana plants confirmed its involvement in the cold acclimation and freezing tolerance.


Assuntos
Ciclopropanos/metabolismo , Etilenos/antagonistas & inibidores , Malus/fisiologia , Doenças das Plantas/imunologia , Reguladores de Crescimento de Planta/antagonistas & inibidores , Transcriptoma , Aclimatação , Temperatura Baixa , Resistência à Doença , Etilenos/metabolismo , Frutas/genética , Frutas/imunologia , Frutas/metabolismo , L-Iditol 2-Desidrogenase/genética , Malus/genética , Malus/imunologia , Modelos Biológicos , Reguladores de Crescimento de Planta/metabolismo , Plantas Geneticamente Modificadas , Metabolismo Secundário , Análise de Sequência de RNA , Sorbitol/metabolismo
17.
Arerugi ; 66(10): 1244-1247, 2017.
Artigo em Japonês | MEDLINE | ID: mdl-29249759

RESUMO

Pectin is used in several foods as an additive and a thickner. But some cases of anaphylaxis have been reported. Most of these are induced by occasional exposures; however, no cases of anaphylaxis after eating a Citrus unshiu, the albedo of which is rich in pectin, have been reported.A 7-year-old girl developed barking cough and pruritus approximately two hours after eating a frozen Citrus unshiu. She had a history of anaphylaxis induced by consuming cashew nuts. Skin testing and basophil activation tests were performed using a commercially available pectin product. Both tests were positive. In an oral food challenge test, she felt abdominal pain and nausea only after eating fruit, along with the albedo, of Citrus unshiu. We concluded that this case was induced by pectin present in the albedo of Citrus unshiu, but not by the fruit itself. We should consider that patients with cashew nut allergies have a possibility of pectin allergies as well, and that pectin in the albedo of Citrus unshiu may induce anaphylaxis.


Assuntos
Anafilaxia/imunologia , Citrus/imunologia , Pectinas/imunologia , Basófilos/imunologia , Criança , Feminino , Frutas/imunologia , Humanos , Imunoglobulina E/imunologia
18.
Pediatr Res ; 82(5): 733-740, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29040259

RESUMO

BackgroundIn this study, we aimed to determine whether introducing various allergenic foods during infancy is associated with IgE sensitization at 12 months of age.MethodsDetailed information on feeding practices regarding six possible allergenic foods (fruits, egg white, egg yolk, fish, shellfish, and peanuts) was obtained by administering age-specific questionnaires to parents of infants at ages 6 and 12 months. Fecal secretory IgA (sIgA), fecal eosinophil cationic protein (ECP), and serum levels of total IgE and IgE specific to 20 foods, and IgE specific to 20 inhalant allergens were also quantified at 12 months of age.ResultsAt 12 months of age, infants with IgE sensitization had been introduced to fewer allergenic food items during infancy (3.2±1.4 vs. 3.7±1.3 items). Compared with infants who were given 0-2 allergenic food items, infants introduced to 3-4 or ≥5 allergenic food items showed a significantly lower risk of IgE sensitization (odds ratios (ORs) 0.62 and 0.61, respectively) and lower total IgE levels. In addition, non-introduction of egg white or egg yolk was significantly related to IgE sensitization (ORs 1.41 and 1.26, respectively).ConclusionIncreasing the diversity of allergenic foods in infancy, including fruits, egg white, egg yolk, fish, shellfish, and peanuts, may protect infants from IgE sensitization at 12 months of age.


Assuntos
Alérgenos/administração & dosagem , Dieta , Métodos de Alimentação , Hipersensibilidade Alimentar/prevenção & controle , Imunoglobulina E/sangue , Alimentos Infantis , Administração por Inalação , Fatores Etários , Alérgenos/efeitos adversos , Alérgenos/imunologia , Biomarcadores/sangue , Dieta/efeitos adversos , Proteínas Dietéticas do Ovo/administração & dosagem , Proteínas Dietéticas do Ovo/efeitos adversos , Proteínas Dietéticas do Ovo/imunologia , Fezes/química , Feminino , Hipersensibilidade Alimentar/sangue , Hipersensibilidade Alimentar/diagnóstico , Hipersensibilidade Alimentar/imunologia , Frutas/efeitos adversos , Frutas/imunologia , Humanos , Imunoglobulina A Secretora/sangue , Lactente , Alimentos Infantis/efeitos adversos , Masculino , Razão de Chances , Hipersensibilidade a Amendoim/diagnóstico , Hipersensibilidade a Amendoim/imunologia , Hipersensibilidade a Amendoim/prevenção & controle , Fatores de Risco , Hipersensibilidade a Frutos do Mar/diagnóstico , Hipersensibilidade a Frutos do Mar/imunologia , Hipersensibilidade a Frutos do Mar/prevenção & controle
19.
Pediatr Allergy Immunol ; 28(8): 793-800, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29024078

RESUMO

BACKGROUND: Previous studies have shown that dietary pattern is associated with allergy prevention. METHODS: We conducted a prospective cohort study on all primary schools in Omihachiman City, Shiga Prefecture, Japan. Questionnaires regarding allergic symptoms and diet were distributed to the parents of all 759 7-year-old schoolchildren for 4 consecutive years, from 2011 to 2014. Specific immunoglobulin E to inhalant allergens was measured at 10 years of age. Participants were then categorized as low, medium, or high intake during the study period for four food groups (fruits, vegetables, fish, and beans). Logistic regression analysis was performed to estimate odds ratios and 95% confidence intervals. RESULTS: A total of 520 children (68.5%) whose parents responded to the questionnaires all 4 years were included in the analysis. The prevalence of asthma, rhinitis, and any allergic symptoms at age 10 was significantly decreased with increases in fruit intake. In addition, the onset of any allergic symptoms during the study period was significantly decreased with increases in fruit intake (33.3%, 28.3%, and 14.3% in children with low, medium, and high fruit intake, respectively; P for trend =.01). The sensitization rate to ragweed at age 10 was significantly decreased with increases in fruit intake (P for trend =.046). No significant effect was observed for the other three food groups, except for the association between fish intake and new-onset asthma symptoms. CONCLUSIONS: These findings suggest that higher intake of fruit can help prevent respiratory allergic symptoms in schoolchildren.


Assuntos
Dieta , Frutas/imunologia , Hipersensibilidade Respiratória/prevenção & controle , Criança , Inquéritos sobre Dietas , Feminino , Humanos , Japão/epidemiologia , Modelos Logísticos , Estudos Longitudinais , Masculino , Razão de Chances , Prevalência , Estudos Prospectivos , Fatores de Proteção , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/etiologia
20.
Plant Sci ; 264: 57-68, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28969803

RESUMO

Allergy to freshly consumed apple fruits is often associated to pollinosis and manifested as oral allergy syndrome (OAS). The allergenic properties of apple varieties differ greatly, spanning from low allergenic to high allergenic varieties. The knowledge of the genetic determinants for allergenicity has been of great interest in scientific community for several years, but the molecular mechanisms involved are still little understood. Here, factors putatively involved in allergenicity were investigated at biochemical and molecular level in pollen and in fruits of apple varieties differing in their allergenic potential. Among putative sensitizing factors, transglutaminase (TGase) and phospholipase A2 (PLA2) were considered together with reactive oxygen species (ROS) and known apple allergen genes, with particular attention devoted to the Mal d 1 gene family, the most important one in sensitization. We found that the expression of some allergen genes and the activities of TGase, PLA2 and ROS producing enzyme are lower in the hypo-allergenic variety 'Durello di Forlì' in comparison with the high-allergenic genotypes 'Gala' and 'Florina'. These results highlight correlations among allergen expressions, enzymatic activities and apple cultivars; these data underline the possibility that some of them could be used in the future as markers for allergenicity.


Assuntos
Alérgenos/genética , Hipersensibilidade Alimentar , Malus/genética , Alérgenos/efeitos adversos , Alérgenos/imunologia , Frutas/efeitos adversos , Frutas/genética , Frutas/imunologia , Humanos , Malus/efeitos adversos , Malus/imunologia , Pólen/efeitos adversos , Pólen/genética , Pólen/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA