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1.
Bratisl Lek Listy ; 121(2): 117-121, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32115963

RESUMO

AIM: The aim of this study is to demonstrate whether fullerenol C60 protects renal injury in sevoflurane­administered rats. METHOD: Rats (n: 24) were randomly divided into four groups: Control (Group C), Fullerenol C60 (Group F), Sevoflurane (Group S), Fullerenol C60-Sevoflurane (Group FS). Thirty minutes before the procedure, Fullerenol C60, 100 mg/kg, was administered intraperitoneally. Sevoflurane (2.3 %) was applied for 3 hours to rats in S and FS groups. Biochemical and histopathological parameters were analyzed in renal tissue samples. Kruskal-Wallis and Mann-Whitney U tests were used in statistical analyzes. RESULTS: Malondialdehyde (MDA) level and catalase (CAT) enzyme activity in Group S were significantly higher than that in all other groups. Paraoxanase (PON) enzyme activity in Group S was significantly lower than in Groups C and FS. The histopathological examination showed that vascular vacuolization and hypertrophy (VVH) and lymphocyte infiltration (LI) were significantly higher in the Group S compared to the Group C. CONCLUSION: Renal histopathology revealed that the administration of Fullerenol C60 prior to sevoflurane inhalation reduced oxidative stress and partially corrected the damage caused by anesthesia. We concluded that Fullerenol C60 has a renal protective effect in rats when administered before sevoflurane anesthesia (Tab. 2, Fig. 4, Ref. 40).


Assuntos
Fulerenos , Rim , Nanopartículas , Animais , Fulerenos/farmacologia , Rim/efeitos dos fármacos , Malondialdeído , Estresse Oxidativo , Ratos , Sevoflurano
2.
Talanta ; 206: 120225, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514839

RESUMO

In this study, we developed an impedimetric sensor system by using molecularly cortisol imprinted acrylamide polymers on fullerene modified carbon electrode to detect cortisol in real saliva samples. The polymer layer was formed on fullerene modified screen printed carbon electrodes with the ratio of 1:2 (cortisol:acrylamide) and ammonium persulfate (APS) was used as the initiator of polymerization. The sensor surface was investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Scanning Electron Microscopy (SEM). Performances of the electrode were determined as follows; calibration curve was calculated between 0.5 nM and 64 nM, R2 = 0.9939, LOD and LOQ were 0.14 nM and 0.44 nM, respectively. Real human saliva samples were obtained from 15 participants between 25 and 32 ages regardless of gender. The samples, which were collected at least 30 min after waking up and the new sensor method analysis were compared tandem mass spectroscopy coupled liquid chromatography system (LC-MS/MS). The sensor analysis showed a significant correlation with LC-MS/MS results (R2 = 0.9727).


Assuntos
Acrilamida/química , Fulerenos/química , Hidrocortisona/análise , Saliva/química , Espectroscopia Dielétrica/instrumentação , Espectroscopia Dielétrica/métodos , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Humanos , Hidrocortisona/química , Limite de Detecção , Impressão Molecular/métodos , Análise de Regressão
3.
Chem Commun (Camb) ; 56(8): 1179-1182, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31868184

RESUMO

We report unprecedented Friedel-Crafts arylation of chlorofullerenes C60Cl6 and C70Cl8 with unprotected carboxylic acids as an efficient single-step synthesis of the inherently stable water-soluble fullerene derivatives. Using this method, a series of previously unaccessible compounds was obtained without chromatographic purification in almost quantitative yields. Promising anti-HIV activity comparable to characteristics of commercial drugs was demonstrated for some of these compounds.


Assuntos
Fármacos Anti-HIV/farmacologia , Ácidos Carboxílicos/farmacologia , Fulerenos/farmacologia , Água/química , Fármacos Anti-HIV/síntese química , Fármacos Anti-HIV/química , Derivados de Benzeno/síntese química , Derivados de Benzeno/química , Derivados de Benzeno/farmacologia , Ácidos Carboxílicos/síntese química , Ácidos Carboxílicos/química , Linhagem Celular Tumoral , Fulerenos/química , HIV-1/efeitos dos fármacos , HIV-2/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Solubilidade , Tiofenos/síntese química , Tiofenos/química , Tiofenos/farmacologia
4.
Chemosphere ; 239: 124728, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31499314

RESUMO

Numerous of pollutants threaten our planet, for instance plastic wastes causes a huge potential risk on the environment in addition to many of emergened pollutants as pharmaceutical residue in aquatic environments which affecting ecological balance and in-turn affecting human health. Accordingly, this research proposed an innovative facile, one-step synthesis of functionalized magnetic fullerene nanocomposite (FMFN) via catalytic thermal decomposition of sustainable poly (ethylene terephthalate) bottle wastes as feedstock and ferrocene as a catalyst and precursor of magnetite. Growth mechanism of FMFN was discussed and batch experiments were achieved to examine its adsorption efficiency in relation to Ciprofloxacin antibiotic. Different adsorption parameters including time, initial Ciprofloxacin concentration, and solution temperature were investigated and optimized using Response Surface Methodology (RSM) model. In addition, a study on the antibiotic adsorption process impact on the organisms of an ecosystem was conducted using E. coli DH5α, and results validated method's efficiency in overcoming problem of appearance of antibiotic-resistant microbes.


Assuntos
Ciprofloxacino/isolamento & purificação , Fulerenos/química , Nanocompostos/química , Poluentes Químicos da Água/isolamento & purificação , Adsorção , Ciprofloxacino/química , Ecossistema , Escherichia coli , Concentração de Íons de Hidrogênio , Cinética , Fenômenos Magnéticos , Modelos Estatísticos , Plásticos , Polietilenotereftalatos/química , Tomografia por Emissão de Pósitrons , Reciclagem , Temperatura Ambiente , Poluentes Químicos da Água/química , Purificação da Água/métodos , Purificação da Água/estatística & dados numéricos
5.
Yakugaku Zasshi ; 139(12): 1539-1546, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31787641

RESUMO

Fullerenes, large spherical molecules composed solely of carbon atoms, have gathered much attention for practical applications that take advantage of their unique spherical structure, physical properties, and biological activities. For example, fullerene C60 can function as a photosensitizer, an antioxidant, a bioimaging agent, and as a gene or drug carrier. However, the practical use of C60 for these potential biomedical applications has been hampered by the fact that it is only sparingly soluble in water. In this review, we focus on the development of hydrophilic C60 nanoparticles, the surface of which is covered by cyclodextrin (CD), and then evaluate its biological activities. C60/CD nanoparticles were stable under physiological conditions, and even under much harsher conditions. The nanoparticles generate reactive oxygen species (ROS) under visible light irradiation. Efficient photodynamic therapy against tumor growth was achieved by the intravenous injection of C60/CD nanoparticles to tumor bearing mice, followed by photoirradiation. In addition, C60(OH)10, which is regarded as a potential candidate for use in scavenging ROS, was also prepared in the form of water soluble nanoparticles. C60(OH)10/CD nanoparticles protect the liver from injury by the suppression of oxidative stress occurring in the mitochondria, for example, by scavenging ROS such as superoxide anion radicals (O2・-), nitric oxide (NO) and peroxynitrite (ONOO-), which act as critical mediators in liver injuries. C60-based nanoparticles represent a potentially promising material for use in the treatment of cancer and oxidative stress-related diseases, and are promising as well in terms of extensive biological applications.


Assuntos
Fulerenos/química , Nanomedicina , Neoplasias/tratamento farmacológico , Animais , Antioxidantes , Carbono/química , Ciclodextrinas , Depuradores de Radicais Livres , Fulerenos/administração & dosagem , Fulerenos/farmacologia , Humanos , Interações Hidrofóbicas e Hidrofílicas , Injeções Intravenosas , Camundongos , Mitocôndrias/metabolismo , Nanopartículas , Estresse Oxidativo/efeitos dos fármacos , Fotoquimioterapia , Espécies Reativas de Oxigênio
6.
J Agric Food Chem ; 67(49): 13509-13517, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31725280

RESUMO

In this study, novel water-soluble quaternary ammonium salts of iminofullerenes (IFQA) were synthesized by nitrene chemistry in combination with quaternization and identified as [C60(NCH2CH2NH3+·CF3COO-)4·10H2O]n by various spectroscopies. Maize and Arabidopsis seeds were used to test the bioactivity of IFQA in seed germination. Compared with the control, maize seed exposure to 50 mg/L IFQA (normal: 73.1% vs 58.7%; drought: 66.7% vs 50.0% at the second day) and Arabidopsis seed exposure to 20 mg/L IFQA (normal: 77.5% vs 58.8%; drought: 63.3% vs 36.7% at the second day) had higher germination rates and quicker germination. The results of two-dimensional gel electrophoresis combined with mass spectroscopy showed that the abundance of 21 proteins in embryo proteome of maize seeds was significantly changed (>1.5 fold). The downregulated six storage proteins and upregulated four proteins induced by IFQA for energy production and sugar metabolism indicated a faster metabolic activity of maize seed germination. The upregulated eight stress-related proteins and antioxidant enzymes suggested that the role of IFQA was to activate the metabolic processes in seed germination and also increase seed stress response. The results provide important information to understand the mechanism of seed germination enhancement by carbon nanomaterials.


Assuntos
Compostos de Amônio/farmacologia , Arabidopsis/crescimento & desenvolvimento , Fulerenos/farmacologia , Reguladores de Crescimento de Planta/farmacologia , Sementes/efeitos dos fármacos , Triticum/crescimento & desenvolvimento , Compostos de Amônio/química , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Arabidopsis/metabolismo , Fulerenos/química , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Germinação/efeitos dos fármacos , Reguladores de Crescimento de Planta/síntese química , Reguladores de Crescimento de Planta/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Triticum/efeitos dos fármacos , Triticum/genética , Triticum/metabolismo
7.
Int J Mol Sci ; 20(19)2019 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-31569423

RESUMO

Photodynamic therapy is an emerging treatment of tumor diseases. The complexes with γ-cyclodextrins (γ-CD) and fullerenes or their derivatives can be used as photosensitizers by direct injection into cancer cells. Using molecular mechanics and molecular dynamics methods, the stability and the geometry of the 2:1 complexes [(γ-CD)2/C70] are investigated analyzing the differences with the analogous C60 complexes, studied in a previous theoretical work and experimentally found to be much less efficient in cancer therapy. The inclusion complex of γ-CD and C70 has a 2:1 stoichiometry, the same as C60, but is significantly less stable and displays an unlike arrangement. In vacuo, mimicking an apolar solvent, the complex is compact, whereas in water the two γ-CDs encapsulate C70 forming a relatively stable complex by interacting through their primary rims, however exposing part of C70 to the solvent. Other higher-energy complexes with the γ-CDs facing different rims can form in water, but in all cases part of the hydrophobic C70 surface remains exposed to water. The stability and arrangement of these peculiar amphiphilic inclusion complexes having non-covalent interactions in water can be an important key for cancer therapy to enhance both the solubilization and the fullerene insertion into liposomes or cell membranes.


Assuntos
Fulerenos/química , Simulação de Dinâmica Molecular , Fármacos Fotossensibilizantes/química , gama-Ciclodextrinas/química , Conformação Molecular , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Análise Espectral , Relação Estrutura-Atividade , gama-Ciclodextrinas/farmacologia
8.
Phys Chem Chem Phys ; 21(42): 23329-23337, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31616869

RESUMO

Interactions between fullerene C24 and a frequently used supplement for sport activities, ephedrine (EPH), have been studied in detail by a combination of density functional theory (DFT), time dependent DFT (TD-DFT) calculations, the symmetry-adapted perturbation theory (SAPT) approach and molecular dynamics (MD) simulations. Information about interaction energies and non-covalent interactions formed between C24 and EPH have been obtained by DFT calculations. TD-DFT calculations have been used in order to obtain UV/vis spectra and to check whether the presence of the EPH molecule produces significant changes in the spectrum. The SAPT approach has been employed in order to decompose the interaction energy into components and therefore to better understand the physical origins of interaction between C24 and EPH. Last, but not least, MD simulations have been used in order to track the influence of temperature on the interactions between C24 and EPH.


Assuntos
Efedrina/química , Fulerenos/química , Sítios de Ligação , Teoria da Densidade Funcional , Simulação de Dinâmica Molecular , Temperatura Ambiente , Termodinâmica
9.
Nat Commun ; 10(1): 4599, 2019 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601813

RESUMO

Host-guest interactions are of central importance in many biological and chemical processes. However, the investigation of the formation and decomplexation of host-guest systems at the single-molecule level has been a challenging task. Here we show that the single-molecule conductance of organoplatinum(II) metallocycle hosts can be enhanced by an order of magnitude by the incorporation of a C60 guest molecule. Mechanically stretching the metallocycle-C60 junction with a scanning tunneling microscopy break junction technique causes the release of the C60 guest from the metallocycle, and consequently the conductance switches back to the free-host level. Metallocycle hosts with different shapes and cavity sizes show different degrees of flexibility to accommodate the C60 guest in response to mechanical stretching. DFT calculations provide further insights into the electronic structures and charge transport properties of the molecular junctions based on metallocycles and the metallocycle-C60 complexes.


Assuntos
Fulerenos/química , Compostos Organoplatínicos/química , Teoria da Densidade Funcional , Eletrodos , Ouro , Espectroscopia de Ressonância Magnética , Microscopia de Força Atômica , Microscopia de Tunelamento , Espectrofotometria Ultravioleta
10.
Environ Pollut ; 255(Pt 1): 113181, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31522006

RESUMO

Extensive application of fullerene nanoparticles (nC60) leads to potential environmental pollution. The acute toxic effects of nC60 have been largely investigated, but studies of behavioural and chronic toxicity at sublethal doses are still rare and the underlying molecular mechanisms remain unknown. The present study investigated behavioural and chronic toxicity of nC60 to Daphnia magna. The results showed that, in response to nC60 exposure, hopping, heartbeat frequencies and feeding ability of D. magna decreased significantly, displaying negative relationship with exposure time and dose. Chronic treatments with 0.1 mg/L or 1 mg/L nC60 for 21 days significantly reduced survival and reproduction of D. magna. These harmful effects suggested negative impacts of nC60 on aquatic ecosystems. Moreover, transcriptome analysis showed that the behavioural and chronic toxicity of nC60 to D. magna might be related to physiological functions such as cell structural repair, protein degradation, energy metabolism and reproduction. We found that nC60 accumulated in guts of D. magna, which should be responsible for the decrease of food ingestion and consequently inhibiting energy intake. Deficiency of energy not only affects behaviours but also declines reproduction in D. magna. Overall, this is the first study comprehensively considered the behavioral and chronic toxicity of nC60 to aquatic organism. The results should be helpful to better understand the ecological consequences of C60 released into water environments.


Assuntos
Daphnia/efeitos dos fármacos , Fulerenos/toxicidade , Nanopartículas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Comportamento Animal/efeitos dos fármacos , Daphnia/genética , Daphnia/fisiologia , Ecossistema , Comportamento Alimentar/efeitos dos fármacos , Fulerenos/química , Perfilação da Expressão Gênica , Nanopartículas/química , Reprodução/efeitos dos fármacos
11.
Molecules ; 24(18)2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31540264

RESUMO

New 3D conformers were synthesized to show a nanomolecular configuration with geometrically branched 2-diphenylaminofluorene (DPAF-C2M) chromophores using a symmetrical 1,3,5-triaminobenzene ring as the center core for the connection of three fused DPAF-C2M moieties. The design led to a class of cis-cup-tris[(DPAF-C2M)-C60(>DPAF-C9)] 3D conformers with three bisadduct-analogous cages per nanomolecule facing at the same side of the geometrical molecular cis-cup-shape structure. A sequential synthetic route was described to afford this 3D configurated conformer in a high yield with various spectroscopic characterizations. In principle, a nanostructure with a non-coplanar 3D configuration in design should minimize the direct contact or π-stacking of fluorene rings with each other during molecular packing to the formation of fullerosome array. It may also prevent the self-quenching effect of its photoexcited states in solids. Photophysical properties of this cis-cup-conformer were also investigated.


Assuntos
Fluorenos/síntese química , Fulerenos/química , Transporte de Elétrons , Fluorenos/química , Conformação Molecular , Estrutura Molecular , Estereoisomerismo
12.
Dokl Biochem Biophys ; 487(1): 256-259, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31559592

RESUMO

The effect of aqueous unmodified fullerene C60 dispersions (AFD) at a concentration of 5 mg/L on the total number and metabolic activity of heterotrophic river bacteroplankton was investigated. The stimulating effect of AFD on the number of bacterioplankton and on the activity of electron transport chains in its cells is shown. It is established that, in the presence of AFD, the bactericidal activity of antibiotics decreases. The stimulating effect of fullerene on the natural heterotrophic bacterioplankton, which we discovered, casts doubt on the expediency of using AFD as a bacteriostatic agent.


Assuntos
Fulerenos/química , Plâncton/química , Plâncton/microbiologia , Água/química , Cinética
13.
Mater Sci Eng C Mater Biol Appl ; 105: 110069, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546439

RESUMO

The development of biosensing interfaces based on copolymerization of benzenamine-2,5-di(thienyl)pyrrole (SNS-An) with 3,4-ethylenedioxythiophene (EDOT) is reported. Both homopolymer P(SNS-An) and copolymer P(SNS-An-co-EDOT) films were prepared and evaluated, in terms of biosensing efficiency, upon incorporation of carbon nanoelements (carbon nanotubes and fullerene) and cross-linking of glucose oxidase. The copolymer revealed superior performance as a biosensing interface as compared to the homopolymer structure or previously reported P(SNS) biosensors. The analytical characteristics and stability studies were performed both at cathodic potential, monitoring O2 consumption, as a result of catalytic reaction of glucose oxidase towards glucose and at anodic potential, following the oxidation of the H2O2 produced during the catalytic reaction. Whilst the measurements on the positive side offered an extended linear range (0.01-5.0 mM), the negative side provided sensitivity up to 104.96 µA/mMcm-1 within a shorter range. Detection limits were as low as 1.9 µM with Km value of 0.49 mM. Lastly, the most performant biosensing platforms, including copolymeric structure and CNTs were employed for analysis in real samples.


Assuntos
Aspergillus niger/enzimologia , Técnicas Biossensoriais , Enzimas Imobilizadas/química , Fulerenos/química , Proteínas Fúngicas/química , Glucose Oxidase/química , Glucose/análise , Nanotubos de Carbono/química , Polímeros , Pirróis
14.
Int J Nanomedicine ; 14: 6197-6215, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31496681

RESUMO

Introduction and objective: Degradation of the extracellular matrix (ECM) changes the physicochemical properties and dysregulates ECM-cell interactions, leading to several pathological conditions, such as invasive cancer. Carbon nanofilm, as a biocompatible and easy to functionalize material, could be used to mimic ECM structures, changing cancer cell behavior to perform like normal cells. Methods: Experiments were performed in vitro with HS-5 cells (as a control) and HepG2 and C3A cancer cells. An aqueous solution of fullerene C60 was used to form a nanofilm. The morphological properties of cells cultivated on C60 nanofilms were evaluated with light, confocal, electron and atomic force microscopy. The cell viability and proliferation were measured by XTT and BrdU assays. Immunoblotting and flow cytometry were used to evaluate the expression level of proliferating cell nuclear antigen and determine the number of cells in the G2/M phase. Results: All cell lines were spread on C60 nanofilms, showing a high affinity to the nanofilm surface. We found that C60 nanofilm mimicked the niche/ECM of cells, was biocompatible and non-toxic, but the mechanical signal from C60 nanofilm created an environment that affected the cell cycle and reduced cell proliferation. Conclusion: The results indicate that C60 nanofilms might be a suitable, substitute component for the niche of cancer cells. The incorporation of fullerene C60 in the ECM/niche may be an alternative treatment for hepatocellular carcinoma.


Assuntos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Fulerenos/farmacologia , Fase G2/efeitos dos fármacos , Neoplasias Hepáticas/patologia , Mecanotransdução Celular , Nanopartículas/química , Comunicação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citoesqueleto/efeitos dos fármacos , Citoesqueleto/metabolismo , Módulo de Elasticidade , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Fulerenos/química , Humanos , Integrina alfa5beta1/metabolismo , Neoplasias Hepáticas/ultraestrutura , Mecanotransdução Celular/efeitos dos fármacos , Nanopartículas/ultraestrutura , Proteínas de Neoplasias/metabolismo , Transdução de Sinais/efeitos dos fármacos
15.
Int J Nanomedicine ; 14: 6325-6337, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31496689

RESUMO

Purpose: We have previously reported that some cationic fullerene derivatives exhibited anticancer activity, and they are expected to be a potential lead compound for an anti-drug resistant cancer agent. However, they are bis-adducts and a mixture of multiple regioisomers, which cannot be readily separated due to the variability of substituent positions on the fullerene cage. To overcome this issue, we evaluated the antiproliferative activities of a set of mono-adduct derivatives and examined their structure-activity relationship. In addition, the in vivo antitumor activity of selected derivatives was also examined. Methods: Nineteen pyridinium fullerene derivatives were newly designed and synthesized in this study. Their antiproliferative activities were evaluated using several cancer cell lines including drug-resistant cells. Furthermore, in vivo antitumor activity of several derivatives was investigated in mouse xenograft model of human lung cancer. Results: The derivatives inhibited the proliferation of cancer cell lines, including cisplatin-resistant cells and doxorubicin-resistant cells. It was also shown that compound 10 (10 µM), 13 (10 µM) and cis-14 (10 µM) induced the intracellular oxidative stress. In addition, compound 13 (20 mg/kg) and cis-14 (15 mg/kg) significantly exhibited antitumor activity in mouse xenograft model of human lung cancer. Conclusion: We synthesized a novel set of mono-adduct fullerene derivatives functionalized with pyridinium groups and found that most of them show potent antiproliferative activities against cancer cell lines and some of them show significant antitumor activities in vivo. We propose that these fullerene derivatives serve as the lead compounds for a novel type of antitumor agents.


Assuntos
Antineoplásicos/síntese química , Antineoplásicos/farmacologia , Fulerenos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Caspase 7/metabolismo , Proliferação de Células/efeitos dos fármacos , Feminino , Fulerenos/química , Células HL-60 , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Células NIH 3T3 , Estresse Oxidativo/efeitos dos fármacos , Quinidina/farmacologia , Carga Tumoral/efeitos dos fármacos , alfa-Tocoferol/farmacologia
16.
Mater Sci Eng C Mater Biol Appl ; 104: 109945, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31499967

RESUMO

The toxicity of C60(OH)30, C70(OH)30, and C120O(OH)n fullerenols, prepared by a new original method, has been studied. This method allowed us to obtain high-purity fullerenols and eliminate the risks of synthesis of preparations containing insoluble fractions contaminated with impurities such as fullerenes not completely reacted by hydroxylation. All fullerenols were detected inside cultured cells. The MTT assay as well as the analysis of apoptosis and cell cycle showed that С60(ОН)30 and С70(OH)30 are non-toxic for cultured V79 и HeLa cells at concentrations exceeding physiological levels by an order of magnitude. С120O(OH)n caused low toxicity. Studies in Drosophila melanogaster showed that any preparations used did not result in a decreased lifespan or in behavior abnormalities in flies.


Assuntos
Fulerenos/química , Fulerenos/toxicidade , Animais , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Linhagem Celular , Linhagem Celular Tumoral , Cricetulus , Drosophila melanogaster/efeitos dos fármacos , Células HeLa , Humanos , Hidroxilação/efeitos dos fármacos
17.
Int J Mol Sci ; 20(17)2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31480310

RESUMO

Particulate matter (PM), a widespread air pollutant, consists of a complex mixture of solid and liquid particles suspended in air. Many diseases have been linked to PM exposure, which induces an imbalance in reactive oxygen species (ROS) generated in cells, and might result in skin diseases (such as aging and atopic dermatitis). New techniques involving nanomedicine and nano-delivery systems are being rapidly developed in the medicinal field. Fullerene, a kind of nanomaterial, acts as a super radical scavenger. Lower water solubility levels limit the bio-applications of fullerene. Hence, to improve the water solubility of fullerene, while retaining its radical scavenger functions, a fullerene derivative, fullerenol C60(OH)36, was synthesized, to examine its biofunctions in PM-exposed human keratinocyte (HaCaT) cells. The PM-induced increase in ROS levels and expression of phosphorylated mitogen-activated protein kinase and Akt could be inhibited via fullerenol pre-treatment. Furthermore, the expression of inflammation-related proteins, cyclooxygenase-2, heme oxygenase-1, and prostaglandin E2 was also suppressed. Fullerenol could preserve the impaired state of skin barrier proteins (filaggrin, involucrin, repetin, and loricrin), which was attributable to PM exposure. These results suggest that fullerenol could act against PM-induced cytotoxicity via ROS scavenging and anti-inflammatory mechanisms, and the maintenance of expression of barrier proteins, and is a potential candidate compound for the treatment of skin diseases.


Assuntos
Poluição do Ar/prevenção & controle , Fulerenos/análise , Material Particulado/toxicidade , Água/química , Apoptose/efeitos dos fármacos , Linhagem Celular , Cidades , Fulerenos/química , Humanos , Inflamação/patologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Queratinócitos/ultraestrutura , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Modelos Biológicos , Fosforilação/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Solubilidade
18.
Molecules ; 24(17)2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31480572

RESUMO

A simple and straightforward synthesis of diporphyrins and pentaporphyrins is reported here. The supramolecular interactions of the new porphyrin derivatives with C60 and PyC60 (a pyridyl [60]fulleropyrrolidine) were evaluated by absorption and fluorescence titrations in toluene. While no measurable modifications of the absorption and fluorescence spectra were observed upon addition of C60 to the porphyrin derivatives, the addition of PyC60 to the corresponding mono-Zn(II) porphyrins resulted in the formation of Zn(porphyrin)-PyC60 coordination complexes and the binding constants were calculated. Results show that the four free-base porphyrin units in pentaporphyrin 6 have a significant contribution in the stabilization of the 6-PyC60 complex. The crystal and molecular features of the pentaporphyrin Zn5 were unveiled using single-crystal X-ray diffraction studies.


Assuntos
Fulerenos/química , Substâncias Macromoleculares/química , Porfirinas/síntese química , Cinética , Substâncias Macromoleculares/síntese química , Modelos Moleculares , Porfirinas/química , Espectrometria de Fluorescência , Tolueno/química
19.
Chem Commun (Camb) ; 55(77): 11511-11514, 2019 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-31490471

RESUMO

A short metallofullere-peapod of Y2@C79N⊂[4]CHBC was constructed. The strong confinement effect from the large π-extended [4]CHBC nanoring induces molecular orientation of the wrapped Y2@C79N, which can be sensed by a Y2@C79N spin probe. The low susceptibility of the spin phase memory time (Tm) for the Y2@C79N spin was also found in a confined space.


Assuntos
Complexos de Coordenação/química , Fulerenos/química , Hidrocarbonetos Cíclicos/química , Marcadores de Spin , Ítrio/química , Compostos Aza/química , Teoria da Densidade Funcional , Conformação Molecular , Transição de Fase , Temperatura Ambiente , Termodinâmica
20.
Int J Mol Sci ; 20(18)2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31509958

RESUMO

The current paper reviews the applications of luminescence bioassays for monitoring the results of low-intensity exposures which produce a stimulative effect. The impacts of radioactivity of different types (alpha, beta, and gamma) and bioactive compounds (humic substances and fullerenols) are under consideration. Bioassays based on luminous marine bacteria, their enzymes, and fluorescent coelenteramide-containing proteins were used to compare the results of the low-intensity exposures at the cellular, biochemical, and physicochemical levels, respectively. High rates of luminescence response can provide (1) a proper number of experimental results under comparable conditions and, therefore, proper statistical processing, with this being highly important for "noisy" low-intensity exposures; and (2) non-genetic, i.e., biochemical and physicochemical mechanisms of cellular response for short-term exposures. The results of cellular exposures were discussed in terms of the hormesis concept, which implies low-dose stimulation and high-dose inhibition of physiological functions. Dependencies of the luminescence response on the exposure time or intensity (radionuclide concentration/gamma radiation dose rate, concentration of the bioactive compounds) were analyzed and compared for bioassays of different organization levels.


Assuntos
Exposição Ambiental/análise , Monitoramento Ambiental/métodos , Luminescência , Medições Luminescentes/métodos , Bactérias/metabolismo , Bactérias/efeitos da radiação , Fulerenos/metabolismo , Fulerenos/efeitos da radiação , Substâncias Húmicas/efeitos da radiação , Proteínas Luminescentes/metabolismo , Radiação Ionizante , Espectrometria de Fluorescência/métodos
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