Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 56
Filtrar
1.
Biomedica ; 39(4): 631-638, 2019 12 01.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31860175

RESUMO

Pediculosis capitis is the most frequent ectoparasitosis around the world. The infestation is caused by Pediculus humanus capitis (head louse), which affects hair, scalp, and skin. It rarely presents with more symptoms and in the majority of the cases, it shows a benign course if treated properly. We present the case of a nursery school girl with head lice infestation for 18 months. She did not improve after the shampoo treatment. This case was associated with furunculosis, skin lesions, multiple adenopathies, and anemia. Initially, the presence of boils, alopecia, and lymphadenopathy was evident. The persistence of pediculosis capitis and intense scratching induced changes on skin integrity, facilitating opportunistic bacterial superinfection that led to impetiginization, furunculosis, excoriations, hematic scabs, anemia, alopecia, and lymphadenopathies. Pediculosis capitis affected the patient triggering psychological, economic, social, and other health problems. The patient presented uncommon symptoms (furunculosis, anemia, fever, alopecia, and adenopathies) resulting from the persistence of risk factors and the absence of head inspection and mechanical removal of insects. The education about the risk factors, as well as sanitary controls, are essential to contain the infestation.


Assuntos
Infestações por Piolhos/complicações , Pediculus , Alopecia/etiologia , Anemia/etiologia , Animais , Pré-Escolar , Dermatoses Faciais/etiologia , Feminino , Furunculose/etiologia , Furunculose/patologia , Humanos , Resistência a Inseticidas , Inseticidas/administração & dosagem , Infestações por Piolhos/diagnóstico , Infestações por Piolhos/terapia , Linfadenopatia/etiologia , Permetrina/administração & dosagem , Pobreza , Prurido/etiologia , Escolas Maternais
2.
J Clin Invest ; 128(8): 3595-3604, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-30035749

RESUMO

Autosomal dominant hyper IgE syndrome (AD-HIES), or Job's syndrome, is a primary immune deficiency caused by dominant-negative mutations in STAT3. Recurrent Staphylococcus aureus skin abscesses are a defining feature of this syndrome. A widely held hypothesis that defects in peripheral Th17 differentiation confer this susceptibility has never been directly evaluated. To assess the cutaneous immune response in AD-HIES, we induced suction blisters in healthy volunteers (HVs) and patients with AD-HIES and then challenged the wound with lethally irradiated bacteria. We show that cutaneous production of IL-17A and IL-17F was normal in patients with AD-HIES. Overproduction of TNF-α differentiated the responses in AD-HIES from HVs. This was associated with reduced IL-10 family signaling in blister-infiltrating cells and defective epithelial cell function. Mouse models of AD-HIES recapitulated these aberrant epithelial responses to S. aureus and involved defective epithelial-to-mesenchymal transition (EMT) rather than a failure of bacterial killing. Defective responses in mouse models of AD-HIES and primary keratinocyte cultures from patients with AD-HIES could be reversed by TNF-α blockade and by drugs with reported modulatory effects on EMT. Our results identify these as potential therapeutic approaches in patients with AD-HIES suffering S. aureus infections.


Assuntos
Células Epiteliais/imunologia , Furunculose/imunologia , Síndrome de Job/imunologia , Queratinócitos/imunologia , Staphylococcus aureus/imunologia , Fator de Necrose Tumoral alfa/imunologia , Adulto , Animais , Modelos Animais de Doenças , Células Epiteliais/patologia , Transição Epitelial-Mesenquimal/genética , Transição Epitelial-Mesenquimal/imunologia , Feminino , Furunculose/genética , Furunculose/patologia , Humanos , Interleucina-17/genética , Interleucina-17/imunologia , Síndrome de Job/genética , Síndrome de Job/patologia , Queratinócitos/patologia , Masculino , Camundongos , Camundongos Transgênicos , Fator de Necrose Tumoral alfa/genética
4.
Biosci Biotechnol Biochem ; 80(6): 1205-13, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27023331

RESUMO

Houttuynia cordata (HC) has been commonly used as many traditional remedies in local areas of Japan. Although many pharmacological activities of HC have been reported, the mechanism underlying the effect of HC remains unknown. We conducted the interview survey in Japan to verify how HC was actually used. The interview survey revealed that HC poultice (HCP) prepared from smothering fresh leaves of HC was most frequently used for the treatment of purulent skin diseases including furuncle and carbuncle with high effectiveness. Ethanol extract of HCP (eHCP) showed anti-bacterial effects against methicillin-resistant Staphylococcus aureus (MRSA), and showed an anti-biofilm activity against MRSA. eHCP showed dose-dependent inhibition of S. aureus lipoteichoic acid (LTA)-induced interleukin-8 and CCL20 production in human keratinocyte without any cytotoxicity. These results suggest that HCP is effective for skin abscess and its underlying mechanism might be the complicated multiple activities for both bacteria and host cells.


Assuntos
Antibacterianos/farmacologia , Anti-Inflamatórios não Esteroides/uso terapêutico , Carbúnculo/tratamento farmacológico , Furunculose/tratamento farmacológico , Houttuynia/química , Fitoterapia/estatística & dados numéricos , Extratos Vegetais/farmacologia , Infecções Estafilocócicas/tratamento farmacológico , Idoso , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Carbúnculo/metabolismo , Carbúnculo/patologia , Linhagem Celular Transformada , Quimiocina CCL20/biossíntese , Quimiocina CCL20/metabolismo , Relação Dose-Resposta a Droga , Etanol , Feminino , Furunculose/metabolismo , Furunculose/patologia , Humanos , Interleucina-8/biossíntese , Interleucina-8/metabolismo , Japão , Queratinócitos/citologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/imunologia , Lipopolissacarídeos/farmacologia , Masculino , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Folhas de Planta/química , Infecções Estafilocócicas/metabolismo , Infecções Estafilocócicas/patologia , Inquéritos e Questionários , Ácidos Teicoicos/farmacologia
5.
Genet Mol Res ; 14(3): 10743-51, 2015 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-26400303

RESUMO

Pathological scar tissues and normal skin tissues were differentiated by screening for differentially expressed genes in pathologic scar tissues via gene expression microarray. The differentially expressed gene data was analyzed by gene ontology and pathway analyses. There were 5001 up- or down-regulated genes in 2-fold differentially expressed genes, 956 up- or down-regulated genes in 5-fold differentially expressed genes, and 114 up- or down-regulated genes in 20-fold differentially expressed genes. Therefore, significant differences were observed in the gene expression in pathological scar tissues and normal foreskin tissues. The development of pathological scar tissues has been correlated to changes in multiple genes and pathways, which are believed to form a dynamic network connection.


Assuntos
Cicatriz/genética , Foliculite/genética , Furunculose/genética , Proteínas/genética , Adulto , Cicatriz/etiologia , Cicatriz/metabolismo , Cicatriz/patologia , Foliculite/complicações , Foliculite/metabolismo , Foliculite/patologia , Prepúcio do Pênis/citologia , Prepúcio do Pênis/metabolismo , Furunculose/complicações , Furunculose/metabolismo , Furunculose/patologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Ontologia Genética , Humanos , Masculino , Redes e Vias Metabólicas/genética , Anotação de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Proteínas/metabolismo
6.
J Proteomics ; 122: 41-54, 2015 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-25849251

RESUMO

UNLABELLED: Furunculosis caused by Aeromonas salmonicida subsp. salmonicida is an epidemic disease among salmonids, including rainbow trout (Oncorhynchus mykiss). However, the immune mechanisms that are elicited in rainbow trout against the invasion of A. salmonicida are not yet fully understood. In this study, we examined the spleen to investigate the immune response of rainbow trout at 3days post-infection by A. salmonicida at the transcriptome and proteome levels by using Illumina-seq and iTRAQ methods, respectively. A total of 1036 genes and 133 proteins were found to undergo differential expression during the immune response of the spleen against A. salmonicida infection. Gene ontology and KEGG analysis were conducted among the differentially expressed genes and proteins, revealing that immune system process and response to stimulus were the top two biological processes, and immune system, signaling molecules and interaction, and immune diseases were the differential pathways activated. Correlation analysis of transcriptomic and proteomic results showed 17 proteins (11 upregulated and 6 downregulated) having consistent expression at RNA and protein levels. Moreover, protein-protein interaction analysis showed that diseases, proteasome, aminoacyl-tRNA biosynthesis, and nucleotide metabolism were the main interactions among the consistently expressed proteins. Consequently, these upregulated proteins, namely, ferritin, CD209, IL13Rα1, VDAC2, GIMAP7, PSMA1, and two ANXA11s could be considered as potential biomarkers for rainbow trout immune responses. BIOLOGICAL SIGNIFICANCE: This study provides the first identification of immune markers through an analysis of the differential expression of both genes and their corresponding protein products in the spleen of rainbow trout after infection by A. salmonicida, shedding light on the molecular mechanisms triggered in rainbow trout against A. salmonicida infection and providing new molecular targets for further immunological research in fish.


Assuntos
Aeromonas salmonicida/imunologia , Proteínas de Peixes/imunologia , Furunculose/imunologia , Regulação da Expressão Gênica/imunologia , Oncorhynchus mykiss/imunologia , Baço/imunologia , Animais , Furunculose/microbiologia , Furunculose/patologia , Perfilação da Expressão Gênica , Oncorhynchus mykiss/microbiologia , Proteômica
7.
J Am Vet Med Assoc ; 246(5): 522-9, 2015 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-25671284

RESUMO

OBJECTIVE: To describe clinical and histopathologic features of furunculosis in dogs following water immersion or exposure to grooming products. DESIGN: Retrospective case series. ANIMALS: 22 dogs with skin lesions consistent with furunculosis and a history of water immersion or grooming prior to onset. Procedures-Information collected from the medical records of affected dogs included signalment, clinical signs, bathing or grooming procedure, diagnostic tests, treatment, and outcome. RESULTS: German Shepherd Dogs (4/22 [18%]) and Labrador Retrievers (4/22 [18%]) were most commonly affected. Skin lesions, particularly hemorrhagic pustules and crusts, were dorsally located in all dogs and occurred a median of 2 days (range, 1 to 7 days) following water immersion or exposure to grooming products. Twenty (91%) dogs were bathed at home or at a commercial grooming facility prior to lesion onset; 1 dog developed skin lesions following hydrotherapy on an underwater treadmill, and 1 dog developed peri-incisional skin lesions after surgery. Lethargy, signs of neck or back pain, and fever were common clinical signs. Pseudomonas aeruginosa was the most common bacterial isolate from dogs with bacteriologic culture performed on skin samples (10/14). The main histologic feature was acute follicular rupture in the superficial dermis with suppurative inflammation and dermal hemorrhage. Systemic antimicrobial treatment, particularly oral administration of fluoroquinolones, resulted in excellent clinical response in 16 of 22 (73%) dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Acute-onset furunculosis with characteristic clinical and histopathologic features in dogs following water immersion or exposure to grooming products was described. Knowledge of the historical and clinical features of this syndrome is essential for accurate diagnosis and appropriate treatment of affected dogs.


Assuntos
Banhos , Doenças do Cão/etiologia , Furunculose/microbiologia , Asseio Animal , Administração Oral , Administração Tópica , Animais , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/uso terapêutico , Doenças do Cão/tratamento farmacológico , Doenças do Cão/patologia , Cães , Feminino , Furunculose/tratamento farmacológico , Furunculose/patologia , Masculino , Pele/patologia
9.
Klin Khir ; (4): 53-5, 2014 Apr.
Artigo em Ucraniano | MEDLINE | ID: mdl-25097980

RESUMO

The results of investigation of the dietotherapy impact on the course of purulent-septic process in soft tissues were analyzed. There were operated 58 patients, to 38 of them a certain diet was prescribed. Immediate impact of the diet therapy on the wound process course was established, what was demonstrated by reduction of duration of the earning capacity loss in patients, who have followed a special diet, by 2.58 days.


Assuntos
Abscesso/dietoterapia , Dieta , Furunculose/dietoterapia , Hidradenite/dietoterapia , Placa Aterosclerótica/dietoterapia , Infecções dos Tecidos Moles/dietoterapia , Abscesso/patologia , Abscesso/cirurgia , Ácidos Graxos Ômega-3/administração & dosagem , Furunculose/patologia , Furunculose/cirurgia , Hidradenite/patologia , Hidradenite/cirurgia , Humanos , Extratos Vegetais/administração & dosagem , Placa Aterosclerótica/patologia , Placa Aterosclerótica/cirurgia , Infecções dos Tecidos Moles/patologia , Infecções dos Tecidos Moles/cirurgia , Resultado do Tratamento , Viburnum/química , Vitaminas/administração & dosagem , Cicatrização/efeitos dos fármacos
13.
J Fish Dis ; 36(11): 949-63, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23444966

RESUMO

Michigan's fisheries rely primarily upon the hatchery propagation of salmonid fish for release in public waters. One limitation on the success of these efforts is the presence of bacterial pathogens, including Aeromonas salmonicida, the causative agent of furunculosis. This study was undertaken to determine the prevalence of A. salmonicida in Michigan fish, as well as to determine whether biochemical or gene sequence variability exists among Michigan isolates. A total of 2202 wild, feral and hatchery-propagated fish from Michigan were examined for the presence of A. salmonicida. The examined fish included Chinook salmon, Oncorhynchus tshawytscha (Walbaum), coho salmon, O. kisutcha (Walbaum), steelhead trout, O. mykiss (Walbaum), Atlantic salmon, Salmo salar L., brook trout, Salvelinus fontinalis (Mitchill), and yellow perch, Perca flavescens (Mitchill). Among these, 234 fish yielded a brown pigment-producing bacterium that was presumptively identified as A. salmonicida. Further phenotypic and phylogenetic analyses identified representative isolates as Aeromonas salmonicida subsp. salmonicida and revealed some genetic and biochemical variability. Logistic regression analyses showed that infection prevalence varied according to fish species/strain, year and gender, whereby Chinook salmon and females had the highest infection prevalence. Moreover, this pathogen was found in six fish species from eight sites, demonstrating its widespread nature within Michigan.


Assuntos
Aeromonas salmonicida/genética , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/microbiologia , Furunculose/veterinária , Aeromonas salmonicida/classificação , Aeromonas salmonicida/efeitos dos fármacos , Animais , Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Feminino , Doenças dos Peixes/patologia , Furunculose/epidemiologia , Furunculose/microbiologia , Furunculose/patologia , Masculino , Michigan/epidemiologia , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Filogenia , Prevalência , Salmonidae , Homologia de Sequência , Fatores Sexuais
15.
Dermatology ; 224(2): 110-4, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22508068

RESUMO

Axillary web syndrome (AWS) is defined as a cord-like structure extending from the axilla to the medial arm following axillary surgery in women with breast cancer. There is only limited literature on the pathogenesis of this syndrome and the etiology of the cord. A 57-year-old man presented with a band-like skin depression and tightness over the medial aspect of his arm extending from the axilla to the antecubital fossa following development of a furuncle in the ipsilateral axilla. Histopathologic examination of the 'band' revealed fibroblastic proliferation surrounding the lymphatic vessel which was identified by presence of an obvious valve as well as positive staining for D2-40, a specific marker for lymphatic endothelium. This is the first report of AWS following axillary furunculosis. This case adds to the limited data on the histopathology of AWS, further confirming the etiology of the 'cord' to be of lymphatic origin.


Assuntos
Furunculose/diagnóstico , Doenças Linfáticas/diagnóstico , Antibacterianos/uso terapêutico , Braço/microbiologia , Braço/patologia , Doxiciclina/uso terapêutico , Feminino , Furunculose/tratamento farmacológico , Furunculose/microbiologia , Furunculose/patologia , Humanos , Doenças Linfáticas/tratamento farmacológico , Doenças Linfáticas/patologia , Masculino , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Pessoa de Meia-Idade , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico , Síndrome , Resultado do Tratamento
16.
Dermatol Online J ; 17(10): 28, 2011 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-22031654

RESUMO

Erythema ab igne is a reticulated, erythematous or hyperpigmented dermatosis that results from chronic and repeated exposure to low levels of infrared radiation. Multiple heat sources have been reported to cause this condition, which include heated reclining chairs, heating pads, hot water bottles, car heaters, electric space heaters, and, more recently, laptop computers. Treatment consists of withdrawing the inciting heat source. Although erythema ab igne carries a good prognosis, it is not necessarily a self-limited diagnosis as patients are at long-term risk of developing subsequent cutaneous malignant conditions, which include squamous cell and merkel-cell carcinomas.


Assuntos
Eritema/etiologia , Furunculose/etiologia , Calefação/instrumentação , Temperatura Alta/efeitos adversos , Transtornos da Pigmentação/etiologia , Biópsia , Carcinoma de Célula de Merkel/etiologia , Carcinoma de Células Escamosas/etiologia , Suscetibilidade a Doenças , Eritema/patologia , Feminino , Furunculose/patologia , Humanos , Perna (Membro) , Pessoa de Meia-Idade , Transtornos da Pigmentação/patologia , Neoplasias Cutâneas/etiologia
17.
Dermatology ; 222(3): 282-4, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21613779

RESUMO

BACKGROUND: About 90% of patients with hidradenitis suppurativa (HS) are smokers. A crucial eliciting role of smoking appears to be certain. In order to increase the awareness of this avoidable major cause of the disease and to facilitate the recognition of incipient disorder, we propose the more specific term 'smoker's boils' for HS lesions of patients who are smokers. METHOD: Clinical pictures of early lesions are presented. For the exceptional cases occurring in nonsmokers, the traditional name HS, representing an umbrella term, is adequate. RESULT/CONCLUSION: The renaming of HS as smoker's boils has the practical advantage that a correct diagnosis can be made at an incipient stage of the disorder. If patients stop smoking at such an early stage of HS, they most likely have a chance that this devastating disease will not progress.


Assuntos
Furunculose/classificação , Hidradenite Supurativa/classificação , Fumar/efeitos adversos , Furunculose/etiologia , Furunculose/patologia , Hidradenite Supurativa/etiologia , Hidradenite Supurativa/patologia , Humanos
18.
Arch Dermatol ; 147(4): 454-8, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21482895

RESUMO

BACKGROUND: Mycobacterium bolletii and Mycobacterium massiliense are recently described species of nontuberculous mycobacteria. Footbaths preceding pedicures at nail salons have been implicated as reservoirs of infection with nontuberculous mycobacteria. To our knowledge, this case series represents the first documented outbreak of M bolletii/M massiliense furunculosis, identified by heat-shock protein 65 gene, hsp65, sequencing, occurring in immunocompetent patrons of a North Carolina nail salon. OBSERVATIONS: We describe 3 cases of lower extremity furunculosis caused by M bolletii/M massiliense associated with pedicure footbaths from the same North Carolina nail salon. Lesions developed within 1 month of the salon visit and were characterized by erythematous, indurated papules and plaques. Histologic examination revealed suppurative granulomatous dermatitis. Mycobacterium bolletii/M massiliense was identified by sequencing the 16S ribosomal RNA (rRNA) and hsp65 genes. All 3 patients responded to different combinations of clarithromycin, doxycycline hydrochloride, azithromycin, and moxifloxacin hydrochloride for complete lesion resolution. CONCLUSIONS: Clinicians should elicit a history of pedicure footbaths and maintain a high level of suspicion when faced with skin lesions of the lower extremities that are culture negative or are refractory to conventional antibiotic therapy. Accurate identification and discrimination of M massiliense and M bolletii is difficult and requires sequencing of multiple gene targets beyond their identical 16S rRNA sequences.


Assuntos
Banhos/efeitos adversos , Técnicas Cosméticas/efeitos adversos , Dermatoses do Pé/microbiologia , Furunculose/microbiologia , Infecções por Mycobacterium/microbiologia , Mycobacterium/classificação , Adolescente , Adulto , Antibacterianos/uso terapêutico , Compostos Aza/uso terapêutico , Azitromicina/uso terapêutico , Proteínas de Bactérias/genética , Chaperonina 60/genética , Feminino , Fluoroquinolonas , Dermatoses do Pé/tratamento farmacológico , Dermatoses do Pé/patologia , Furunculose/tratamento farmacológico , Furunculose/patologia , Humanos , Moxifloxacina , Mycobacterium/genética , Mycobacterium/isolamento & purificação , Infecções por Mycobacterium/tratamento farmacológico , Infecções por Mycobacterium/patologia , Quinolinas/uso terapêutico , Resultado do Tratamento , Adulto Jovem
20.
J Fish Dis ; 33(5): 441-9, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20298453

RESUMO

We report the first isolation, identification and characterization of a group of Chilean strains of atypical Aeromonas salmonicida isolated from freshwater farmed Atlantic salmon, Salmo salar. Affected fish showed superficial ulcers and pale liver with or without petechial haemorrhages. Outbreaks of the disease occurred in two farms in the south of Chile about 2200 km apart. Five strains were isolated in pure culture and identified by serological assays and immunofluorescence tests as belonging to Aeromonas salmonicida. Although the bacterial isolates were phenotypically homogeneous, minor differences with the reference strain A. salmonicida subsp. salmonicida ATCC 33658 were noted. Three specific primer sets and partial 16S rRNA gene sequencing allowed the identification of the Chilean isolates as atypical A. salmonicida, with A. salmonicida subsp. achromogenes and A. salmonicida subsp. masoucida as their closest relatives (100% sequence similarity). Molecular typing indicated that the atypical isolates belong to two genetic groups that were associated with the geographical origin.


Assuntos
Aeromonas salmonicida/genética , Doenças dos Peixes/microbiologia , Doenças dos Peixes/patologia , Água Doce , Furunculose/veterinária , Aeromonas salmonicida/isolamento & purificação , Animais , Chile , Furunculose/microbiologia , Furunculose/patologia , Genes Bacterianos/genética , Dados de Sequência Molecular , Fenótipo , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , Salmo salar
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA