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1.
Arch Virol ; 164(11): 2837-2841, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31494776

RESUMO

Since January 2019, abnormal molting has been observed frequently in approximately 40-day-old Pekin ducks in China. To investigate the possible involvement of a virus, we tested the prevalence of duck circovirus (DuCV), goose hemorrhagic polyomavirus (GHPyV), and goose parvovirus (GPV) in 11 molt cases in two provinces. GPV was detected in all cases, particularly in all samples collected from the feather area. The complete genome sequences of three GPV strains were determined and found to have 52 nucleotide changes relative to GPVs associated with short beak and dwarfism syndrome of Pekin ducks. These data will enhance our understanding of GPV diversity and outcomes of GPV infection in Pekin ducks.


Assuntos
Patos/virologia , Gansos/virologia , Muda/fisiologia , Parvovirinae/isolamento & purificação , Doenças das Aves Domésticas/virologia , Animais , China/epidemiologia , Circovirus/genética , Circovirus/isolamento & purificação , Genoma Viral/genética , Parvovirinae/genética , Polyomavirus/genética , Polyomavirus/isolamento & purificação , Doenças das Aves Domésticas/epidemiologia
2.
Avian Pathol ; 48(5): 477-485, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31155930

RESUMO

Hydropericardium hepatitis syndrome (HHS) is a fatal disease in chickens, mainly caused by fowl adenovirus serotype 4 (FAdV-4). Since June 2015, HHS has appeared in many provinces in China. The disease has spread from broilers to laying hens, breeders and Cherry Valley ducks, seriously endangering the health of the poultry industry in China. In July 2016, an infectious disease was noticed in a goose farm in Jinan, Shandong Province, China, and hydropericardium was the main finding in post mortem investigations. In the actual study, we isolated a FAdV-4 strain from the livers of naturally-infected goslings and designated it as SDJN. We first evaluated its pathogenicity by inoculating Taizhou geese at 10, 20, and 30 days of age with 10-7.15EID50/0.2 ml doses of the SDJN strain in 1 ml allantoic fluid via subcutaneous injection or oral infection. Clinical signs and pericardial effusion appeared in geese infected subcutaneously at 10 days of age, whereas 20- and 30-day-old geese were not susceptible to FAdV-4. The results of real-time PCR showed that the replication ability of FAdV-4 in geese correlated with the age. Furthermore, results from clinical chemistry showed that FAdV-4 damaged the liver and kidney in geese and the results paralleled viral load and gross lesions. Consequently, FAdV-4 was pathogenic in geese, and the pathogenicity was related to age and mode of infection. This study is the first experimental infection of FAdV-4 in geese, which will provide a basis for further understanding of the disease. RESEARCH HIGHLIGHTS Pathogenicity tests with a FAdV-4 were conducted in geese, which included data on clinical signs, gross pathology, histopathology, clinical chemistry and viral load. FAdV-4 could replicate in geese and HHS was successfully induced. Pathogenicity of FAdV-4 in geese was related to the age and routes of infection.


Assuntos
Infecções por Adenoviridae/veterinária , Aviadenovirus/patogenicidade , Gansos/virologia , Doenças das Aves Domésticas/virologia , Infecções por Adenoviridae/patologia , Infecções por Adenoviridae/virologia , Animais , Aviadenovirus/genética , Aviadenovirus/imunologia , China , Feminino , Filogenia , Doenças das Aves Domésticas/patologia , Sorogrupo , Carga Viral/veterinária , Virulência
3.
Virology ; 534: 36-44, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31176062

RESUMO

Bangladesh has reported repeated outbreaks of highly pathogenic avian influenza (HPAI) A(H5) viruses in poultry since 2007. Because of the large number of live poultry markets (LPM) relative to the population density of poultry throughout the country, these markets can serve as sentinel sites for HPAI A(H5) detection. Through active LPM surveillance during June 2016-June 2017, HPAI A(H5N6) viruses along with 14 other subtypes of influenza A viruses were detected. The HPAI A(H5N6) viruses belonged to clade 2.3.4.4 and were likely introduced into Bangladesh around March 2016. Human infections with influenza clade 2.3.4.4 viruses in Bangladesh have not been identified, but the viruses had several molecular markers associated with potential human infection. Vigilant surveillance at the animal-human interface is essential to identify emerging avian influenza viruses with the potential to threaten public and animal health.


Assuntos
Vírus da Influenza A/isolamento & purificação , Influenza Aviária/virologia , Doenças das Aves Domésticas/virologia , Animais , Bangladesh/epidemiologia , Surtos de Doenças , Patos , Evolução Molecular , Gansos/virologia , Vírus da Influenza A/classificação , Vírus da Influenza A/genética , Influenza Aviária/epidemiologia , Filogenia , Doenças das Aves Domésticas/epidemiologia
4.
Acta Trop ; 196: 93-101, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31063711

RESUMO

The purpose of this case study is to examine how environmental disruption and agricultural practices act synergistically to create a perfect storm for the spread of avian influenza. Actors in this case study include the vast permafrost landscape of the Qinghai-Tibet Plateau; a wild goose that migrates over the Himalayas; the highest altitude railway in the world that traverses the plateau into Tibet; and an avian virus (H5N1). Commencing in 2001, tens of thousands of railway workers travelled to remote regions of the plateau to work on the railway. In order to feed and shelter these workers, the Chinese government established captive-bred goose farms as a source of high protein food. Beginning in 2005 and continuing in subsequent years, Qinghai Lake was the scene for the unprecedented appearance of avian influenza among migratory geese. This was a key moment in the global spread of H5N1 to poultry on three continents. Remote sensing technology suggested an ecological pathway for the transfer of avian viruses among chickens, captive-bred geese, and wild geese. Within a region experiencing rapid climate change, Qinghai Lake is warming even faster than the global average. This may relate to the persistent outbreaks of avian flu strains from Qinghai during the past twelve years. Globally, exponential increases in bird flu outbreaks are not merely a matter of chance mutations in flu viruses but also a result of antecedent social and environmental factors. The Qinghai case study provides real-world examples that bring these factors into sharp focus.


Assuntos
Galinhas/virologia , Gansos/virologia , Virus da Influenza A Subtipo H5N1 , Influenza Aviária/epidemiologia , Animais , Surtos de Doenças/veterinária , Influenza Aviária/virologia , Filogenia , Tibet/epidemiologia
5.
Arch Virol ; 164(5): 1405-1410, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30847609

RESUMO

During the surveillance of avian influenza viruses in East Dongting Lake, China (2014-2015), two H11N8 avian influenza viruses were detected in the bean goose (Anser fabalis) and the falcated teal (Anas falcata). Phylogenetic analysis showed that these two novel reassortant H11N8 avian influenza viruses contain genes from poultry and wild birds. This is the first report detecting the H11N8 subtype influenza virus from wild birds in Asia. These findings indicate a dissemination of avian influenza virus along the East Asian-Australian flyway. In addition, the interaction between poultry and wild birds was addressed suggesting the need for intensive surveillance of wild bird populations.


Assuntos
Patos/virologia , Gansos/virologia , Glicoproteínas de Hemaglutininação de Vírus da Influenza/classificação , Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Vírus da Influenza A/isolamento & purificação , Neuraminidase/classificação , Neuraminidase/genética , Animais , Animais Selvagens/virologia , Aves/virologia , China , Genoma Viral/genética , Vírus da Influenza A/classificação , Vírus da Influenza A/genética , Influenza Aviária/virologia , Filogenia , Aves Domésticas/virologia , Vírus Reordenados/classificação , Vírus Reordenados/genética
6.
BMC Vet Res ; 15(1): 63, 2019 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-30782148

RESUMO

BACKGROUND: Short beak and dwarfism syndrome (SBDS) was caused by novel goose parvovirus (NGPV)--a variant of goose parvovirus (GPV). Ducks infected with NGPV shows clinical signs including growth retardation and protrusion of the tongue from an atrophied beak. SBDS outbreak was first reported at the northern coastal provinces of China during 2015 and it was again reported in Sichuan, an inland province of China in 2016. The disease caused a huge economic loss in Chinese duck feeding industry. RESULTS: The SD15 strain of NGPV was isolated from liver and intestinal tract tissue samples of infected ducks. Real-time quantitative PCR (qPCR) was used to estimate viral load in embryonated eggs and cells infected with adapted virus. The data showed that duck embryo fibroblasts (DEFs) were permissive to NGPV, while goose embryo fibroblasts (GEFs) cells were not, and the copy numbers of SD15 in the allantoic fluid of infected eggs remained at 105.0-106.5 copies/ml. The adaption procession of the virus was determined via qPCR, and viral proliferation was detected through indirect fluorescent antibody assay (IFA) in DEFs. It was further determined that viral copy numbers peaked at 96 h post-inoculation (hpi), which is the best time to harvest the virus in DEFs. Cytotoxic effects and cell death were observed at 72 hpi in SD15 infected DEFs, yet SD15 did not induce apoptosis. CONCLUSIONS: The growth characteristics of SD15 strain of NGPV determined would be beneficial for further molecular characterization of these viruses and develop potential vaccines if required.


Assuntos
Parvovirinae/crescimento & desenvolvimento , Animais , Patos/virologia , Fibroblastos/virologia , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Gansos/virologia , Técnicas In Vitro , Óvulo/virologia , Parvovirinae/isolamento & purificação , Parvovirinae/fisiologia , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Replicação Viral
7.
Virol J ; 16(1): 6, 2019 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-30630503

RESUMO

BACKGROUND: Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important. RESULTS: A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded. CONCLUSION: This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.


Assuntos
Patos/virologia , Gansos/virologia , Infecções por Parvoviridae/veterinária , Parvovirus/classificação , Doenças das Aves Domésticas/diagnóstico , Reação em Cadeia da Polimerase em Tempo Real/métodos , Animais , Cloaca/virologia , Genoma Viral , Compostos Orgânicos , Orofaringe/virologia , Infecções por Parvoviridae/diagnóstico , Parvovirus/isolamento & purificação , Filogenia , Doenças das Aves Domésticas/virologia , Temperatura de Transição , Carga Viral
8.
Infect Genet Evol ; 67: 1-6, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30366084

RESUMO

Since 2017, a new type of goose-origin astrovirus (GoAstV) disease occurred in China. This disease can cause joint swelling of sick geese, and the anatomy shows a clear urate precipitation in the viscera. The rate of death or amputation can reach more than 30%, revealing its severe pathogenicity. One novel goose-origin astrovirus strain, designated as CXZ18, was isolated from diseased geese with a fatal infection characterized by visceral urate deposition. Similar clinical anatomy symptoms were partially reproduced by attacking infection of healthy geese. The CXZ18 has no hemagglutination with chicken erythrocyte, only reproduced in goose embryos, not in SPF chicken or duck embryos. The complete genome-encoded three open reading frames (ORFs) of CXZ18 were 7252 nt in length. BLAST-based homology analysis of viral complete genome showed that CXZ18 has only 53.0%-61.8% with other classic avian astrovirus from various hosts. Further analysis of ORF 1a, ORF 1b, and ORF 2 genes revealed that the isolate was genetically distinct from known astroviruses and belonged to a distinctive branch of avian astroviruses. To conclude, a naturally occurring novel nephrotic astrovirus, distinguished with all previously reported avian astroviruses, was derived from goose.


Assuntos
Infecções por Astroviridae/veterinária , Avastrovirus/classificação , Avastrovirus/genética , Gansos/virologia , Genoma Viral , Genômica , Doenças das Aves Domésticas/virologia , Animais , Avastrovirus/ultraestrutura , China , Biologia Computacional/métodos , Genômica/métodos , Filogenia , Aves Domésticas , Doenças das Aves Domésticas/diagnóstico
9.
Vet Microbiol ; 227: 69-77, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30473354

RESUMO

A severe infectious disease characterized by nephritis, hepatitis and splenitis has attacked goslings around Shandong province in China since 2016. A novel chicken-origin avian orthoreovirus (ARV) was isolated with LMH cells from affected goslings named Reo/Goose/SDPY/1116/17 (SDPY-ARV) strain, and the infection was successfully reproduced experimentally. The ARV-SDPY full genome sequencing was conducted using Next-Generation Sequencing (NGS) technique on Illumina HiSeq platform. The complete genome of SDPY-ARV was 23,427 bp in length and consist of 10 dsRNA segments ranged from 1192 bp (S4) to 3958 bp (L1) which encoding 12 viral proteins. Genomic sequence analysis showed that the SDPY-ARV strain is in the same branch with broiler, pheasant-origin ARV isolates, and shares 51.8-96.2% of nucleotide identity of σC gene with them; while only 49.3-50.3% with waterfowl isolates. In addition, the occurrence of 10 segments genetic reassortment of SDPY strain is confirmed among the PA15511, the 1733 and the PA13649 strains from America. In conclusion, the causative agent of gosling hemorrhagic necrotic hepatitis and nephritis occurring in China is a novel chicken-origin goose orthoreovirus.


Assuntos
Gansos/virologia , Genoma Viral , Hepatite Viral Animal/etiologia , Orthoreovirus Aviário/genética , Orthoreovirus Aviário/isolamento & purificação , Doenças das Aves Domésticas/virologia , Infecções por Reoviridae/veterinária , Fatores Etários , Animais , Galinhas , China/epidemiologia , Genômica , Hepatite Viral Animal/epidemiologia , Hepatite Viral Animal/virologia , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Fígado/patologia , Fígado/virologia , Necrose/veterinária , Necrose/virologia , Fases de Leitura Aberta , Orthoreovirus Aviário/fisiologia , Filogenia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/transmissão , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/transmissão , Infecções por Reoviridae/virologia , Análise de Sequência de DNA , Proteínas Virais/genética
10.
Pol J Vet Sci ; 21(2): 389-396, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30450880

RESUMO

In order to compare the pathogenicity of different Tembusu virus (TMUV) strains from geese, ducks and chickens, 56 5-day-old Cherry Valley ducklings which were divided into 7 groups and infected intramuscularly with 7´105 PFU/ml per duck of six challenge virus stocks. The clinical signs, weight gain, mortality, macroscopic and microscopic lesions, virus loads in sera of 1, 3, 5, 7, 11 and 14 dpi and serum antibody titers were examined. The results showed that these viruses could make the young ducks sick, but the clinical signs differed with the different species-original strains. All the experimental groups lose markedly in weight gain compared to the control, but there were no obvious distinctions in weight gains, as well as macroscopic and microscopic lesions of dead ducks between the infected groups. However, the groups of waterfowl-derived strains (from geese and ducks) showed more serious clinical signs and higher relative expressions of virus loads in sera than those from chicken-derived. The mortality of waterfowl groups was 37.5%, and the greatest mortality of chicken groups was 12.5%. The serum antibodies of the geese-species group JS804 appeared earlier and were higher in the titers than others. Taken toghter, the pathogenicity of waterfowl-derived TMUV was more serious than chicken-derived TMUV and JS804 could be chosen as one TMUV vaccine strain to protect from the infection.


Assuntos
Infecções por Flavivirus , Flavivirus , Doenças das Aves Domésticas , Animais , Galinhas/virologia , Patos/virologia , Flavivirus/patogenicidade , Infecções por Flavivirus/veterinária , Gansos/virologia , Doenças das Aves Domésticas/virologia
11.
J Vet Sci ; 19(6): 782-787, 2018 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-30304886

RESUMO

Goose hemorrhagic polyomavirus (GHPV) is not a naturally occurring infection in geese in China; however, GHPV infection has been identified in Pekin ducks, a domestic duck species. Herein, we investigated the prevalence of GHPV in five domestic duck species (Liancheng white ducks, Putian black ducks, Shan Sheldrake, Shaoxing duck, and Jinyun Sheldrake) in China. We determined that the Jinyun Sheldrake duck species could be infected by GHPV with no clinical signs, whereas no infection was identified in the other four duck species. We sequenced the complete genome of the Jinyun Sheldrake origin GHPV. Genomic data comparison suggested that GHPVs share a conserved genomic structure, regardless of the host (duck or geese) or region (Asia or Europe). Jinyun Sheldrake origin GHPV genomic characterization and epidemiological studies will increase our understanding of potential heterologous reservoirs of GHPV.


Assuntos
Patos/virologia , Gansos/virologia , Polyomaviridae/genética , Infecções por Polyomavirus/veterinária , Doenças das Aves Domésticas/virologia , Infecções Tumorais por Vírus/veterinária , Animais , China/epidemiologia , Genoma Viral/genética , Filogenia , Reação em Cadeia da Polimerase/veterinária , Infecções por Polyomavirus/genética , Infecções por Polyomavirus/virologia , Doenças das Aves Domésticas/epidemiologia , Prevalência , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterinária , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologia
12.
Cytokine ; 111: 325-333, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30269029

RESUMO

TRAF family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1) serves as hub molecule at the crossroad of multiple signaling pathways of type I interferon (IFN) induction. The importance of TBK1 in innate immunity has been demonstrated in mammalian, however the characterization and function of TBK1 in avian remains largely unknown. In this study, we cloned duck TBK1 (duTBK1) from duck embryo fibroblasts (DEFs) for the first time, which encoded 729 amino acids and had a high amino acid identity with goose and cormorant TBK1s. The duTBK1 showed a diffuse cytoplasmic localization in DEFs and was extensively expressed in all tested tissues. Overexpression of duTBK1 induced IFN-ß production through the activation of IRF1 and NF-κB in DEFs. The N-terminal kinase domain and the ubiquitin-like domain in middle of duTBK1 played pivotal roles in IFN-ß induction as well as in IRF1 and NF-κB activation. Furthermore, knockdown of duTBK1 by small interfering RNA significantly decreased poly(I:C)- or Sendai virus (SeV)-induced IFN-ß expression. In addition, duTBK1 expression dramatically reduced the replication of both duck reovirus (DRV) and duck Tembusu virus (DTMUV) in DEFs. These results suggested that the duTBK1 played a pivotal role in mediating duck antiviral innate immunity.


Assuntos
Proteínas Aviárias/imunologia , Patos/imunologia , Interferon beta/imunologia , Sequência de Aminoácidos , Aminoácidos/imunologia , Animais , Linhagem Celular , Patos/virologia , Fibroblastos/imunologia , Fibroblastos/virologia , Flavivirus/imunologia , Gansos/imunologia , Gansos/virologia , Imunidade Inata/imunologia , Fator Regulador 1 de Interferon/imunologia , NF-kappa B/imunologia , Poli I-C/imunologia , Proteínas Serina-Treonina Quinases/imunologia , Reoviridae/imunologia , Alinhamento de Sequência , Transdução de Sinais/imunologia
13.
J Vet Med Sci ; 80(12): 1861-1866, 2018 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-30298830

RESUMO

Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) have both been found to cause high mortality and morbidity in Muscovy ducklings. Specific detection is often rife with false positives due to high identity at the genomic nucleotide level and antigenic similarity between MDPVs and GPVs. In this study, significantly variable regions were found, via non-structural (NS) comparison, between MDPV and GPV NS genes; however, NS genes were conserved within the MDPV and GPV groups. A polymerase chain reaction (PCR) assay for detecting and differentiating MDPVs and GPVs was developed with more specificity based on the NS gene characterization. The assay detected as low as 103 DNA copies of both the MDPV and GPV strains, along with 549 separate base pairs (bp). No bands of the same size from other duck pathogens, including duck circovirus, duck enteritis virus, egg drop syndrome virus, duck-origin goose hemorrhagic polyomavirus, Escherichia coli, Salmonella, Riemerella anatipestifer and Pasteurella multocida were amplified. This indicates that this method for performing PCR provides a useful and reliable alternative tool for more precise differentiation of MDPV and GPV infection in clinical samples.


Assuntos
Patos/virologia , Gansos/virologia , Parvovirinae/classificação , Reação em Cadeia da Polimerase/veterinária , Proteínas não Estruturais Virais/genética , Animais , Primers do DNA , Genes Virais , Parvovirinae/genética , Reação em Cadeia da Polimerase/métodos
14.
Mol Cell Probes ; 42: 32-35, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30240819

RESUMO

To obtain a deletion mutant of Muscovy duck-origin goose parvovirus (MDGPV) and to analyze its biological characteristics, the pMDGPVPT plasmid, which contains a full-length DNA infectious clone of the MDGPV PT strain, was used in this study as the template. The E-box at nt 315 of the left inverted terminal repeat sequence (L-ITR) was deleted by overlap extension PCR to obtain the infectious recombinant plasmid p-PTΔE315. The p-PTΔE315 plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac and the rescued deletion mutant virus r-PTΔE315 was generated. Experiments to demonstrate the novel deletion mutant virus' biological characteristics showed that r-PTΔE315 can cause typical lesions after infection of Muscovy duck embryos. Compared with its parent strain PT, the virulence of r-PTΔE315 and its proliferation ability in Muscovy duck embryos were attenuated, but its ability to replicate in MDEF cells was enhanced. This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence.


Assuntos
Patos/virologia , Elementos E-Box/genética , Gansos/virologia , Técnicas Genéticas , Parvovirus/genética , Deleção de Sequência , Animais , Sequência de Bases , Células Clonais
15.
Arch Virol ; 163(12): 3463-3466, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30209584

RESUMO

Avian orthoreovirus (ARV) has been considered as a significant pathogen causing great infectious diseases to the avian, like broiler and waterfowl. The genome of this novel ARV(Reo/SDPY/Goose) was completely sequenced by next-generation sequencing. The complete genome was found to be 23517 bp in length with 10 segments. Although the Reo/SDPY/Goose was isolated from the gosling, it shares great similarity, no matter which segment within the genome, with those published as avian-origin reovirus. Genomic analysis revealed that this virus was distinct from published ARV strains and met criteria to become a novel ARV strain.


Assuntos
Doenças das Aves/virologia , Gansos/virologia , Genoma Viral , Orthoreovirus Aviário/isolamento & purificação , Infecções por Reoviridae/veterinária , Animais , Animais Selvagens/virologia , Sequência de Bases , China , Fases de Leitura Aberta , Orthoreovirus Aviário/classificação , Orthoreovirus Aviário/genética , Filogenia , Infecções por Reoviridae/virologia , Sequenciamento Completo do Genoma
16.
Emerg Microbes Infect ; 7(1): 152, 2018 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-30185786

RESUMO

Since the first isolation from human, astroviruses have been detected in many species. Wide host range and occasional cross-transmission of astrovirus pose a risk for zoonotic infection. Here, novel astroviruses were identified from goslings with recent epidemic gout disease in China. A virus, designated as GD, was efficiently isolated from a diseased gosling using LMH cells. Genome of GD amplified using 5' and 3' RACE was 7183nt in full length. Sequence analysis revealed the genome of GD was <60.8% homology with others deposited in Genbank. Moreover, GD could be neutralized by goose convalescent sera, and the gout associated symptom in goslings could be reproduced by GD infection. Our data demonstrated the goose astrovirus could be one of the causative agents of the ongoing gosling gout disease in China. The identification of the goose astrovirus not only diversified the astrovirus species, but also broadened the disease patterns caused by astroviruses.


Assuntos
Infecções por Astroviridae/veterinária , Astroviridae/isolamento & purificação , Doenças Transmissíveis Emergentes/veterinária , Gansos/virologia , Doenças das Aves Domésticas/virologia , Animais , Astroviridae/classificação , Astroviridae/genética , Infecções por Astroviridae/epidemiologia , Infecções por Astroviridae/patologia , Infecções por Astroviridae/virologia , China/epidemiologia , Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/patologia , Doenças Transmissíveis Emergentes/virologia , Genoma Viral , Filogenia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/patologia
17.
Infect Genet Evol ; 66: 18-25, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30196122

RESUMO

Avian influenza viruses (AIVs) are genetically separated by geographical barriers, resulting in the independent evolution of North American and Eurasian lineages. In the present study, to determine whether AIVs possessing the North American-origin nonstructural (NS) gene were previously introduced into South Korea, we performed a genetic analysis of AIVs isolated from fecal samples of migratory birds. We detected seven viruses possessing the North American-origin NS allele B among 413 AIV-positive samples obtained during AI surveillance between 2012 and 2017. We found evidence for the intercontinental transmission of at least three genetically distinct clusters of the B allele of the North American-origin NS gene into Eurasia at a low frequency. The host species of three viruses were identified as the greater white-fronted goose (Anser albifrons) using a DNA barcoding technique. Moreover, we used GPS-CDMA-based telemetry to determine the migration route of the greater white-fronted goose between the Far East of Russia and South Korea and found that this species may play an important role as an intermediate vector in the intercontinental transmission of AIVs. To improve our understanding of the role of wild birds in the ecology of AIVs, advanced AIV surveillance is required in the Far East of Russia as well as in Alaska region of Beringia accompanied by host identification and wild bird tracking.


Assuntos
Alelos , Variação Genética , Vírus da Influenza A/classificação , Vírus da Influenza A/genética , Influenza Aviária/epidemiologia , Influenza Aviária/virologia , Proteínas não Estruturais Virais/genética , Migração Animal , Animais , Animais Selvagens , Aves/virologia , Gansos/virologia , Sistemas de Informação Geográfica , Vírus da Influenza A/isolamento & purificação , Influenza Aviária/transmissão , América do Norte/epidemiologia , Filogenia , República da Coreia/epidemiologia
18.
Arch Virol ; 163(12): 3351-3356, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30159683

RESUMO

Flanders virus (FLAV; family Rhabdoviridae) is a mosquito-borne hapavirus with no known pathology that is frequently isolated during arbovirus surveillance programs. Here, we document the presence of FLAV in Culex tarsalis mosquitoes and a Canada goose (Branta canadensis) collected in western North America, outside of the currently recognized range of FLAV. Until now, FLAV-like viruses detected in the western United States were assumed to be Hart Park virus (HPV, family Rhabdoviridae), a closely related congener. A re-examination of archived viral isolates revealed that FLAV was circulating in California as early as 1963. FLAV also was isolated in Nebraska, Colorado, South Dakota, North Dakota, and Saskatchewan, Canada. Phylogenetic analysis of the U1 pseudogene for 117 taxa and eight nuclear genes for 15 taxa demonstrated no distinct clustering between western FLAV isolates. Assuming the range of FLAV has been expanding west, these results indicate that FLAV likely spread west following multiple invasion events. However, it remains to be determined if the detection of FLAV in western North America is due to expansion or is a result of enhanced arbovirus surveillance or diagnostic techniques. Currently, the impact of FLAV infection remains unknown.


Assuntos
Doenças das Aves/virologia , Culex/virologia , Gansos/virologia , Mosquitos Vetores/virologia , Infecções por Rhabdoviridae/veterinária , Rhabdoviridae/isolamento & purificação , Animais , Doenças das Aves/transmissão , América do Norte , Filogenia , Rhabdoviridae/classificação , Rhabdoviridae/genética , Infecções por Rhabdoviridae/transmissão , Infecções por Rhabdoviridae/virologia , Estações do Ano
19.
Avian Pathol ; 47(6): 559-575, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29985640

RESUMO

In May 2016, highly pathogenic avian influenza virus of the subtype A/H5N1 was detected in Cameroon in an industrial poultry farm at Mvog-Betsi, Yaoundé (Centre region), with a recorded sudden increase of deaths among chickens, and an overall mortality rate of 75%. The virus spread further and caused new outbreaks in some parts of the country. In total, 21 outbreaks were confirmed from May 2016 to March 2017 (six in the Centre, six in the West, eight in the South and one in the Adamaoua regions). This resulted in an estimated total loss of 138,252 birds (44,451 deaths due to infection and 93,801 stamped out). Only domestic birds (chickens, ducks and geese) were affected in farms as well as in poultry markets. The outbreaks occurred in three waves, the first from May to June 2016, the second in September 2016 and the last wave in March 2017. The topology of the phylogeny based on the haemagglutinin gene segment indicated that the causative H5N1 viruses fall within the genetic clade 2.3.2.1c, within the same group as the A/H5N1 viruses collected in Niger in 2015 and 2016. More importantly, the gene constellation of four representative viruses showed evidence of H5N1/H9N2 intra-clade reassortment. Additional epidemiological and genetic data from affected countries in West Africa are needed to better trace the origin, spread and evolution of A/H5N1 in Cameroon. RESEARCH HIGHLIGHTS HPAI A/H5N1 was detected in May 2016 in domestic chickens in Yaoundé-Cameroon. Twenty-one outbreaks in total were confirmed from May 2016 to March 2017. The causative H5N1 viruses fall within the genetic clade 2.3.2.1c. The viral gene constellation showed evidence of H5N1/H9N2 intra-clade reassortment.


Assuntos
Virus da Influenza A Subtipo H5N1/genética , Vírus da Influenza A Subtipo H9N2/genética , Influenza Aviária/virologia , Doenças das Aves Domésticas/virologia , Vírus Reordenados/genética , Animais , Camarões/epidemiologia , Galinhas/virologia , Surtos de Doenças/veterinária , Patos/virologia , Gansos/virologia , Virus da Influenza A Subtipo H5N1/patogenicidade , Influenza Aviária/epidemiologia , Filogenia , Aves Domésticas , Doenças das Aves Domésticas/epidemiologia , Vírus Reordenados/patogenicidade
20.
Arch Virol ; 163(10): 2865-2869, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29980852

RESUMO

Since early 2016, the Chinese goose industry has experienced severe outbreaks of gout; however, the etiological factor of the disease is still unclear. Here, we investigated the possible involvement of viral infection in the disease. Using sequence-independent PCR amplification, astrovirus sequences were generated from a gout case. Full-length genomic sequencing and sequence analysis of three goose astrovirus (GoAstV) strains revealed that they belong to a new avastrovirus most closely related to viruses classified within species Avastrovirus 3. The GoAstV was detected in 16/16 gout cases collected from two provinces, supporting a pathogenic role for the new avastrovirus.


Assuntos
Infecções por Astroviridae/veterinária , Avastrovirus/genética , Avastrovirus/isolamento & purificação , Gansos/virologia , Gota/veterinária , Animais , Infecções por Astroviridae/virologia , Avastrovirus/classificação , Patos , Genoma Viral , Gota/virologia , Fases de Leitura Aberta , Filogenia , Doenças das Aves Domésticas/virologia , Perus
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