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1.
Front Immunol ; 13: 941219, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35911675

RESUMO

Cold atmospheric plasma has been widely applied in medical treatment clinically, especially skin diseases. However, the mechanism of cold atmospheric plasma on the treatment of skin diseases is still undefined. In this study, dinitrofluorobenzene-induced atopic dermatitis mice model was constructed. Cold atmospheric plasma was able to decrease skin cells apoptosis, relieve skin inflammation, ER stress and oxidative stress caused by dinitrofluorobenzene stimulation, which was mediated by cold atmospheric plasma-induced MANF expression. In terms of mechanism, hypoxia-inducible factor-1α expression was increased intracellularly after cold atmospheric plasma treatment, which further bound to the promoter region of manf gene and enhanced MANF transcriptional expression. This study reveals that cold atmospheric plasma has a positive effect on atopic dermatitis treatment, also demonstrates the regulatory mechanism of cold atmospheric plasma on MANF expression via HIF-1α, which indicates the potential medical application of cold atmospheric plasma for atopic dermatitis treatment.


Assuntos
Dermatite Atópica , Gases em Plasma , Animais , Dermatite Atópica/induzido quimicamente , Dermatite Atópica/genética , Dermatite Atópica/terapia , Dinitrofluorbenzeno/farmacologia , Estresse do Retículo Endoplasmático , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Camundongos , Fatores de Crescimento Neural/metabolismo , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico
2.
Molecules ; 27(13)2022 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-35807413

RESUMO

Soft tissue sarcomas (STS) are a rare and highly heterogeneous group of solid tumors, originating from various types of connective tissue. Complete removal of STS by surgery is challenging due to the anatomical location of the tumor, which results in tumor recurrence. Additionally, current polychemotherapeutic regimens are highly toxic with no rational survival benefit. Cold atmospheric plasma (CAP) is a novel technology that has demonstrated immense cancer therapeutic potential. Canady Cold Helios Plasma (CHCP) is a device that sprays CAP along the surgical margins to eradicate residual cancer cells after tumor resection. This preliminary study was conducted in vitro prior to in vivo testing in a humanitarian compassionate use case study and an FDA-approved phase 1 clinical trial (IDE G190165). In this study, the authors evaluate the efficacy of CHCP across multiple STS cell lines. CHCP treatment reduced the viability of four different STS cell lines (i.e., fibrosarcoma, synovial sarcoma, rhabdomyosarcoma, and liposarcoma) in a dose-dependent manner by inhibiting proliferation, disrupting cell cycle, and inducing apoptosis-like cell death.


Assuntos
Gases em Plasma , Sarcoma , Neoplasias de Tecidos Moles , Apoptose , Divisão Celular , Humanos , Recidiva Local de Neoplasia , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Sarcoma/tratamento farmacológico , Sarcoma/patologia , Neoplasias de Tecidos Moles/tratamento farmacológico
3.
Proc Natl Acad Sci U S A ; 119(31): e2200708119, 2022 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-35901213

RESUMO

Gas plasma technology generates reactive oxygen and nitrogen species (ROS/RNS), inducing lethal oxidative damage in tumor cells. The transfer of gas plasma-derived ROS/RNS into liquids has been proposed as an innovative anti-cancer strategy targeting peritoneal carcinomatosis (PC). However, the mechanism of action is under debate. To this end, we compared gas plasma-oxidized medical-grade sodium chloride (oxNaCl) with a concentration-matched control (cmc) of NaCl enriched with equivalent concentrations of H2O2 and NO3- in several cell lines and models of PC. Strikingly, oxNaCl and cmc performed equally well in oxidation and cytotoxic activity in tumor cells in two-dimensional cultures, three-dimensional (3D) tumor spheroids, vascularized 3D tumors grown on chicken-embryo chorioallantoic membranes, and a syngeneic PC mouse model in vivo. Given the importance of immunotherapies in oncology today, we focused on immunological consequences of the treatment. Again, to a similar extent, oxNaCl and cmc increased tumor cell immunogenicity and enhanced uptake by and maturation of peripheral blood monocyte-derived dendritic cells together with an inflammatory secretion profile. Furthermore, NanoString gene expression profiling revealed immune system processes and unfolded protein response-related pathways as being linked to the observed anti-tumor effects for both oxNaCl and cmc. In conclusion, gas plasma-generated oxNaCl and cmc showed equal therapeutic efficacy in our PC-related models. In light of the many promising anti-cancer studies of gas plasma-oxidized liquids and the convenient production of corresponding cmcs in large quantities as needed in clinics, our findings may spur research lines based on low-dose oxidants in peritoneal cancer therapy.


Assuntos
Peróxido de Hidrogênio , Neoplasias Peritoneais , Cloreto de Sódio , Animais , Linhagem Celular Tumoral , Embrião de Galinha , Peróxido de Hidrogênio/química , Camundongos , Neoplasias Peritoneais/tratamento farmacológico , Gases em Plasma , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Cloreto de Sódio/farmacologia
4.
Sci Rep ; 12(1): 11127, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35778444

RESUMO

Non-thermal atmospheric pressure plasma (NTAPP) is a partially ionized gas containing fast electrons and relatively slow ions. This study aims to investigate the influences of NTAPP on human adipose tissue-derived stem cells (ADSCs) and examine the feasibility of using optical spectroscopy as a non-destructive method for cell analysis. A plasma jet is used as the source of low-temperature plasma in which pure helium gas is ionized by a high voltage (8 kV) and frequency (6 kHz). ADSCs were exposed to the NTAPP for 30 s, 60 s, 90 s, and 120 s. The efficiency of the plasma treatment was investigated using flow cytometry and optical spectroscopy methods. This study compared surface markers of NTAPP treated and untreated ADSCs using CD90 and CD105 as positive markers. The result proved that NTAPP-exposed ADSCs maintain their stemming. Measuring ADSCS apoptosis by labeling Annexin V-Propidium Iodide showed that the plasma at short exposure time is relatively non-toxic. However, a longer exposure time can lead to apoptosis and necrosis. Moreover, Cell cycle analysis revealed that NTAPP accelerates the cell cycle in very low doses and can cause proliferation. In this experiment, flow cytometry measurements have been used to determine oxidative stress. The results showed that with increasing plasma dose, intracellular ROS levels reduced. This data also suggests that intracellular ROS are not responsible for the cells' viability. Furthermore, we used reflectance spectroscopy as a non-destructive method for evaluating treatment response and comparing this method with cell analysis techniques. The results indicate spectroscopy's efficiency as a method of cell analysis. This study suggests that NTAPP would be an efficient tool to improve ADSCs culture's efficiency in vitro; thus, we support the potential applications of NTAPP in the field of stem cell therapy and regenerative medicine.


Assuntos
Gases em Plasma , Tecido Adiposo/metabolismo , Humanos , Gases em Plasma/química , Gases em Plasma/farmacologia , Espécies Reativas de Oxigênio , Células-Tronco , Antígenos Thy-1/metabolismo
5.
Sci Rep ; 12(1): 11218, 2022 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-35780177

RESUMO

This study aims to improve the quality and quantity of winter wheat by using the potential of combining the use of cold plasma and waste biorefinery products for improving wheat yield. Plasma was applied by a radio frequency (RF) plasma reactor operated with air for 180 s and 50 W. The waste biorefinery products, including pyroligneous acid, biochar, and azolla compost, were used as plant nutrition. The effects of cold plasma treatment and waste biorefinery products were determined by measuring plant photosynthesis, grain yield, and content of chlorophyll, carotenoids, anthocyanin, protein, and starch. The experiment was conducted during the cropping seasons 2016-18 in a randomized complete block design with four replications. The combination of cold plasma and pyroligneous acid increased the grain yield up to 40.0%. The photosynthesis rate was improved up to 39.3%, and total chlorophyll content up to 48.3% in both years. Seed plasma treatment combined with biochar application increased the starch content by 36.8%. Adding azolla compost increased the protein content by 35.4%. Using seed plasma treatment with biochar increased the microbial biomass carbon by 16.0%. The application of plasma and azolla compost increased the microbial biomass nitrogen by 29.0%.


Assuntos
Gases em Plasma , Triticum , Biomassa , Clorofila , Grão Comestível , Estações do Ano , Solo , Amido
6.
Sci Rep ; 12(1): 12069, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35840616

RESUMO

Cold atmospheric plasma has established its effect on cell adhesion. Given the importance of cell adhesion in stem cells, the current study investigates the effect of plasma treatment on Human Bone Marrow Mesenchymal Stem Cells (HBMMSCs) adhesion by which the differentiation and fate of cells are determined. In this paper, adhesion modification is considered not only for cell- ECM (Extra cellular Matrix), but also between suspended cells, and enhanced adhesions were found in both circumstances. Regarding the previous works, the increase of the cell-ECM adhesion during the plasma therapy was mostly attributed to the enhancement of the production and activity of integrin proteins. Nevertheless, considering the importance of van der Waals forces at the cellular level, the effect of cold plasma on VDWFs and so its effect on adhesion is investigated in this work for the first time, to the best of our knowledge. For this purpose, employing the semi-empirical methods, the role of the plasma therapy on the VDWF between the cells has been studied at three levels; (a) plasma-induced dipole formation, (b) Hammaker coefficient modification of culture medium, and c) cell roughness modification. For suspended cell condition, we conclude and support that van der Waals forces (VDWFs) enhancement has a key role in cell adhesion processes. We believe that, the present work gives a new physical insight in studying the plasma therapy method at the cellular level.


Assuntos
Células-Tronco Mesenquimais , Gases em Plasma , Adesão Celular , Diferenciação Celular , Humanos , Integrinas , Gases em Plasma/farmacologia
7.
J Environ Public Health ; 2022: 4515115, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35844936

RESUMO

Zambia and the DR Congo are situated in the central African Copperbelt, which is part of the Lufilian geological structure arc stretching over from Kolwezi in Katanga Province in the DRC to Luanshya in Copperbelt Province in Zambia. The area has large copper-cobalt deposits of which the extraction causes severe ecosystem damage due to pollution of water, food crops, and the ambient air negatively impacting population health. Contamination of drinking water for domestic use and foods (cereals, roots and tubers, vegetables, and fruits) was determined by assessing the contents of trace metals including Mn, Ni, Pb, Zn, Co, As, U, Cd, and Cu and through a questionnaire for environmental damage. Food samples were analyzed by inductively coupled argon plasma/optical emission spectroscopy (ICP-OES), while water and urine samples were analyzed by inductively coupled argon plasma mass spectrometry (ICP-MS). Concentrations of Ni, Pb, and Cd were higher in almost all food crops, although Cu was more in samples of Cucurbita maxima and Amaranthus hybridus. Mean contents (µg/L) of Mn, Zn, Cd, Pb, and U were, respectively, 5,454.6, 2552.2, 138.7, 39.7, 2361.1, and 21.4 in the DRC and 108.9, 543.3, 0.3, 0.2, 1.5, and 0.5 in Zambia, being significantly higher and always far above World Health Organization maximum limits in the DRC. Urine samples taken only from the DRC contained trace metals with children's samples being more contaminated than adult ones. Our results conclusively echo the most critical challenges of toxic pollutants causing numerous health issues among the population. Given an outcry among households adjacent to mines about land degradation and food spoilage, and health problems over years, joint efforts are needed from public and private sectors for stringent mining exploitation monitoring for sustainable governance to protect the environment and ensure food and nutrition safety, and population well-being in Zambia and the DR Congo.


Assuntos
Metais Pesados , Gases em Plasma , Saúde da População , Poluentes do Solo , Oligoelementos , Adulto , Cádmio , Criança , República Democrática do Congo , Ecossistema , Monitoramento Ambiental/métodos , Humanos , Chumbo/análise , Metais Pesados/análise , Gases em Plasma/análise , Poluentes do Solo/análise , Oligoelementos/análise , Água/análise , Zâmbia
8.
Biomolecules ; 12(7)2022 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-35883469

RESUMO

In this study, we combined atmospheric pressure cold plasma, a novel treatment technology, with an absorption technique with soybean husk to remove Pb and Cd from milk. Different combinations of treatment duration, voltage, and post treatment retention time were used to determine the effectiveness of cold plasma. Soybean husk was used for metal extraction, and it was observed that when the milk samples were plasma treated with a discharge voltage of 50 kV for 2 min and held for 24 h, the highest mean elimination of about 27.37% for Pb and 14.89% for Cd was obtained. Reactive oxygen and nitrogen species produced from plasma treatment were identified using Optical Emission Spectra analysis. A high voltage of 50 kV plasma for a 2 min duration could produce 500 ± 100 ppm of ozone concentration inside the treated package. The value of ΔE, which indicates overall color difference measurement, was significantly (p < 0.05) higher in all the treated samples than control samples. However, in the frequency range from 0.01 to 100 Hz, there was not much difference between the control and treated sample in the frequency sweep test. The identified functional groups at different wavenumbers (cm-1) in the treated samples were found to be similar compared to the control samples.


Assuntos
Gases em Plasma , Animais , Cádmio/toxicidade , Chumbo/toxicidade , Leite , Água
9.
Int J Mol Sci ; 23(13)2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35806379

RESUMO

Cold plasma (CP) technology is a technique used to change chemical and morphological characteristics of the surface of various materials. It is a newly emerging technology in agriculture used for seed treatment with the potential of improving seed germination and yield of crops. Wheat seeds were treated with glow (direct) or afterglow (indirect) low-pressure radio-frequency oxygen plasma. Chemical characteristics of the seed surface were evaluated by XPS and FTIR analysis, changes in the morphology of the seed pericarp were analysed by SEM and AFM, and physiological characteristics of the seedlings were determined by germination tests, growth studies, and the evaluation of α-amylase activity. Changes in seed wettability were also studied, mainly in correlation with functionalization of the seed surface and oxidation of lipid molecules. Only prolonged direct CP treatment resulted in altered morphology of the seed pericarp and increased its roughness. The degree of functionalization is more evident in direct compared to indirect CP treatment. CP treatment slowed the germination of seedlings, decreased the activity of α-amylase in seeds after imbibition, and affected the root system of seedlings.


Assuntos
Gases em Plasma , Triticum , Germinação , Gases em Plasma/farmacologia , Plântula , Sementes , Triticum/fisiologia , alfa-Amilases
10.
Food Res Int ; 156: 111300, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35651060

RESUMO

Cellulose has attracted high attention due to its advantages of abundant resources, renewable and biodegradable. Modification of natural plant cellulose has become a hot topic worldwide. Conventional chemical modification methods commonly cause great damage to the environment. The current review presents the effects of innovative, eco-friendly and sustainable nonthermal processing technologies on cellulose structure and properties. Typical techniques include high pressure processing, cold plasma, ultrasonic and irradiation treatment. Their superiorities in the modification of cellulose are highlighted, and the advantages and limitations of nonthermal processing technologies for plant cellulose modification are also discussed. Nonthermal processing technologies can improve cellulose functional properties by playing an important role in the chemical bonds of the molecular chains, crystalline regions or amorphous parts through energy or active particles generated in the process, or promoting the crosslinking and graft copolymerization of cellulose molecules. The development of modified cellulose functional materials will have wider applications.


Assuntos
Celulose , Gases em Plasma , Celulose/química , Fenômenos Químicos , Plantas , Ultrassom
11.
Sci Rep ; 12(1): 9481, 2022 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-35676321

RESUMO

Trichophyton rubrum, a major human pathogenic dermatophyte, is responsible for the most recurrent dermatophytoses as globally important superficial fungal infections. Typical chemotherapy is used to handle such infections; however, emerging drug resistance and side effects necessitate the new remedial method development. Cold atmospheric plasma (CAP) is an emerging technology, consisted of neutral and charged particles and photons newly developed as a potent and safe antimicrobial technique to combat drug-resistant microbial pathogens. In the present study, the vast effects of CAP irradiation containing oxygen (2%) and helium (98%) on T. rubrum growth and pathogenicity were explored. After exposure of T. rubrum to CAP jet for 90, 120, 150, 180, and 210 s in 96-well microtiter plates, cell morphology and viability, ergosterol content of fungal hyphae, HSP90 gene expression, and the pattern of drug susceptibility were studied by using electron microscopy, RT-qPCR, spectrophotometry, disk diffusion and CLSI microbroth dilution methods. CAP irradiation significantly inhibited the fungal growth by 25.83 to 89.10%, reduced fungal cell viability by 11.68 to 87.71%, disrupted cellular membranous organelles and structures of the fungal hyphae, and suppressed efficiently the expression of HSP90 gene by 2 folds in 210 s exposure. Taken together, our results demonstrated that CAP is an efficient tool with potential in-vivo therapeutic applications against chronic dermatophytosis caused by T. rubrum due to its effectiveness, harmless, and ease of access.


Assuntos
Antifúngicos , Arthrodermataceae , Gases em Plasma , Antifúngicos/farmacologia , Arthrodermataceae/efeitos dos fármacos , Arthrodermataceae/crescimento & desenvolvimento , Expressão Gênica , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/metabolismo , Gases em Plasma/farmacologia
12.
Ecotoxicol Environ Saf ; 240: 113703, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35659700

RESUMO

Atmospheric pressure cold plasma (APCP) holds great potential as an efficient, economical and eco-friendly approach for improving crop production. Although APCP-induced plant growth promotion is undisputedly attributed to the reactive oxygen and nitrogen species (RONS), how these RONS regulate the intracellular redox state and plant growth is still largely unknown. This study systematically investigates the regulation mechanism of APCP-generated RONS on intracellular redox homeostasis in Arabidopsis thaliana seedling by measuring the RONS compositions in APCP-treated solutions and intracellular RONS and antioxidants in Arabidopsis seedlings. The results show that APCP exhibited a dual effect (stimulation or inhibition) on Arabidopsis seedling growth dependent on the treatment time. APCP-generated RONS in liquids increased in a time-dependent manner, leading to an increase of conductivity and oxidation reduction potential (ORP) and decrease of pH. APCP caused an enrichment of intracellular RONS and most of them increased with APCP treatment time. Meanwhile, APCP treatment accelerated malondialdehyde (MDA) generation, and the level of intracellular antioxidants were enhanced by low-dose APCP treatment while decreased at high doses. The results of correlation analysis showed that the extracellular RONS produced by APCP were positively correlated with the intracellular RONS and negatively correlated with the antioxidants. These results demonstrate that the improved antioxidant capacity induced by moderate APCP-generated RONS plays an important role in the growth promotion of Arabidopsis seedlings, which may be a promising alternative for fertilizers in agricultural production.


Assuntos
Arabidopsis , Gases em Plasma , Antioxidantes/metabolismo , Arabidopsis/metabolismo , Pressão Atmosférica , Homeostase , Nitrogênio/química , Oxirredução , Oxigênio , Gases em Plasma/farmacologia , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio , Plântula/metabolismo
13.
Naturwissenschaften ; 109(4): 33, 2022 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-35687146

RESUMO

Conventional oligopeptide synthesis techniques involve environmentally harmful procedures and materials. In addition, the efficient accumulation of oligopeptides under Hadean Earth environments regarding the origin of life remains still unclear. In these processes, the formation of diketopiperazine is a big issue due to the strong inhibition for further elongation beyond dipeptides. Hydrothermal media enables environmentally friendly oligopeptide synthesis. However, hydrothermal oligopeptide synthesis produces large amounts of diketopiperazine (DKP), due to its thermodynamic stability. DKP inhibits dipeptide elongation and also constitutes an inhibitory pathway in conventional oligopeptide synthesis. Here, we show an efficient pathway for oligopeptide formation using a specially designed experimental setup to run both thermal and non-thermal discharge plasma, generated by nano-pulsed electric discharge with 16-23 kV voltage and 300-430 A current within ca. 500 ns. DKP (14%) was converted to dipeptides and higher oligopeptides in an aqueous solution containing alanine-DKP at pH 4.5, after 20 min of 50 pps thermal plasma irradiation. This is the first study to report efficient oligopeptide synthesis in aqueous medium using nano-pulsed plasma (with thermal plasma being more efficient than non-thermal plasma) via DKP ring-opening. This unexpected finding is implicative to evaluate the pathway how the oligopeptides could have accumulated in the primitive Earth with high-energy plasma sources such as thunder as well as to facilitate the green synthesis of oligopeptides.


Assuntos
Dicetopiperazinas , Gases em Plasma , Dipeptídeos , Oligopeptídeos , Água
14.
J Water Health ; 20(6): 962-971, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35768970

RESUMO

The main aim of this work is inactivation of Escherichia coli in water using a laboratory-scale radio-frequency atmospheric pressure Argon plasma jet. This bacterium is widely present in the environment, especially in drinking water, and its pathogenic effects are very harmful. For this purpose, an Argon flow rate of 3.5 slm, maximum plasma power of 200 W, and discharge frequency of 13.56 MHz was conducted to generate a uniform plasma plume for water treatment. 150 ml of drinking water contaminated by E. coli was exposed to the radiation of plasma placed about 3 cm within the water, the treatment time varied from 2 to 6 minutes at 100, 150, and 200 W of plasma input power. The temperature of the plume, discharge current and voltage, and electron density were all measured to characterize the plasma. Active species such as excited molecules, ions, and radicals produced in the plasma in water were detected using the optical emission spectroscopy method. The decreasing behavior of live bacteria versus exposure time and plasma jet input power was observed, and finally, at the discharge power of 200 W and 6 min, an effective inactivation was achieved and the number of bacteria reduced from 92×104 to less than 1.7 MPN/100 ml.


Assuntos
Água Potável , Infecções por Escherichia coli , Gases em Plasma , Argônio/química , Argônio/farmacologia , Pressão Atmosférica , Escherichia coli/fisiologia , Humanos , Gases em Plasma/química , Gases em Plasma/farmacologia
15.
Food Res Int ; 157: 111246, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35761559

RESUMO

Climate change increases the need for effective and sustainable technologies in food and agriculture. Plasma-activated water (PAW) emerges as a green and sustainable technology in food processing and production. Synergy of a myriad of reactive oxygen and nitrogen species (RNOS) in PAW contributes to desirable properties of PAW. Compared to conventional methods, PAW is fast and effective for various products, not limited by the volume or shape of the treated samples. In this review, we will first introduce the fundamentals on plasma generation, physicochemical properties and characterization of PAW. Among various approaches for activation improvement, we highlight a recent progress in improving the cold plasma activation by microbubbles. Then we critically review the treatment conditions by PAW, and effectiveness of PAW for bio-film removal, food processing, plant growth in agriculture, and environment. As the research output on PAW is expanding exponentially, this review focuses on the work published within the last two years (2020-2021) to summarize the current understanding of the principles of the effects from PAW and to reflect potentials of PAW in applications for the food science and technology.


Assuntos
Gases em Plasma , Agricultura , Gases em Plasma/química , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio/química , Água/química
16.
Sci Total Environ ; 838(Pt 4): 156576, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35688233

RESUMO

Cold atmospheric plasma (CAP) possesses the ability of high-efficiency disinfection. It is reported that mixtures of reactive oxygen species (ROS) including ·OH, 1O2, O2- and H2O2 generated from CAP have better antimicrobial ability than mimicked solution of mixture of single ROS type, but the reason is not clear. In this study, CAP was applied to treat yeasts in water in order to investigate the fungal inactivation efficiency and mechanism. The results showed that plasma treatment for 5 min could result in >2-log reduction of yeast cells, and application of varied ROS scavengers could significantly increase the yeast survival rate, indicating that ·OH and 1O2 played the pivotal role in yeast inactivation. Moreover, the synergistic effect of 1O2 with other plasma-generated ROS was revealed. 1O2 could diffuse into cells and induce the depolarization of mitochondrial membrane potential (MMP), and different levels of MMP depolarization determined different cell death modes. Mild damage of mitochondria during short-term plasma treatment could lead to apoptosis. For long-term plasma treatment, the cell membrane could be severely damaged by the plasma-generated ·OH, so a large amount of 1O2 could induce more depolarization of MMP, leading to increase of intracellular O2- and Fe2+ which subsequently caused cell inactivation. 1O2 could also induce protein aggregation and increase of RIP1/RIP3 necrosome, leading to necroptosis. With participation of 1O2, endogenous ·OH could also be generated via Fenton and Haber-Weiss reactions during plasma treatment, which potentiated necroptosis. Adding l-His could mitigate membrane damage, inhibit the drop of MMP and the formation of necrosome, and thus prevent the happening of necroptosis. These findings may deepen the understanding of plasma sterilization mechanisms and provide guidance for microbial killing in the environment.


Assuntos
Gases em Plasma , Oxigênio Singlete , Desinfecção/métodos , Peróxido de Hidrogênio , Oxigênio , Espécies Reativas de Oxigênio/metabolismo , Saccharomyces cerevisiae , Água
17.
Head Face Med ; 18(1): 21, 2022 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-35768853

RESUMO

BACKGROUND: The aim of the present study was to examine the cytostatic effects of cold atmospheric plasma (CAP) on different head and neck squamous carcinoma (HNSCC) cell lines either in isolation or in combination with low dose cisplatin. The effect of CAP treatment was investigated by using three different HNSCC cell lines (chemo-resistant Cal 27, chemo-sensitive FaDu and OSC 19). MATERIALS AND METHOD: Cell lines were exposed to CAP treatment for 30, 60, 90, 120 and 180 s (s). Cisplatin was added concurrently (cc) or 24 h after CAP application (cs). Cell viability, DNA damage and apoptosis was evaluated by dye exclusion, MTT, alkaline microgel electrophoresis assay and Annexin V-Fit-C/PI respectively. RESULTS: In all cell lines, 120 s of CAP exposure resulted in a significant reduction of cell viability. DNA damage significantly increased after 60 s. Combined treatment of cells with CAP and low dose cisplatin showed additive effects. A possible sensitivity to cisplatin could be restored in Cal 27 cells by CAP application. CONCLUSION: CAP shows strong cytostatic effects in HNSCC cell lines that can be increased by concurrent cisplatin treatment, suggesting that CAP may enhance the therapeutic efficacy of low dose cisplatin.


Assuntos
Carcinoma de Células Escamosas , Citostáticos , Neoplasias de Cabeça e Pescoço , Gases em Plasma , Carcinoma de Células Escamosas/tratamento farmacológico , Carcinoma de Células Escamosas/genética , Linhagem Celular Tumoral , Cisplatino/farmacologia , Cisplatino/uso terapêutico , Citostáticos/uso terapêutico , Neoplasias de Cabeça e Pescoço/tratamento farmacológico , Humanos , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Carcinoma de Células Escamosas de Cabeça e Pescoço/tratamento farmacológico
18.
Front Immunol ; 13: 868386, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35720416

RESUMO

Skin diseases are mainly divided into infectious diseases, non-infectious inflammatory diseases, cancers, and wounds. The pathogenesis might include microbial infections, autoimmune responses, aberrant cellular proliferation or differentiation, and the overproduction of inflammatory factors. The traditional therapies for skin diseases, such as oral or topical drugs, have still been unsatisfactory, partly due to systematic side effects and reappearance. Cold atmospheric plasma (CAP), as an innovative and non-invasive therapeutic approach, has demonstrated its safe and effective functions in dermatology. With its generation of reactive oxygen species and reactive nitrogen species, CAP exhibits significant efficacies in inhibiting bacterial, viral, and fungal infections, facilitating wound healing, restraining the proliferation of cancers, and ameliorating psoriatic or vitiligous lesions. This review summarizes recent advances in CAP therapies for various skin diseases and implicates future strategies for increasing effectiveness or broadening clinical indications.


Assuntos
Gases em Plasma , Dermatopatias , Humanos , Nitrogênio , Oxigênio , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio , Dermatopatias/tratamento farmacológico
19.
Sci Rep ; 12(1): 9850, 2022 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-35701491

RESUMO

This study aimed to investigate the synergistic effect of the cold atmospheric plasma (CAP) and heterogeneous photocatalytic processes in an aqueous solution to enhance water purification efficacy and reduce the energy cost required by CAP. 0.1% Ag/TiO2-reduced graphene oxide (rGO) nanoparticles (NPs) photo-composite were prepared and fully characterized. Data showed that Ag nanoparticles and the rGO play an important role in increasing the efficiency of the whole treatment process and the photo-composite (0.1% Ag/TiO2-1% rGO at 400 °C) revealed the highest phenol removal rate with excellent reusability. Also, complete inactivation (~ 5log10 reduction) of both E. coli and S. aureus by NPs was observed without CAP exposure, whereas a minimal effect (0.1-0.5 log10) on viruses (Adenovirus (AdV), rotavirus, and ɸX174) was observed after 10 min incubation. Interestingly, the photocatalytic virus inactivation test was promising, as it resulted in > 4.7log10 reduction of AdV at 2 min treatment, whereas < 1log10 could be reduced using only CAP at the same treatment time. Accordingly, we believe that this work could provide new insights into how the synergy between CAP and 0.1% Ag/TiO2-1% rGO photo-composite in aqueous media imposes a great potential for environmental applications, such as water purification and microbial inactivation.


Assuntos
Nanopartículas Metálicas , Gases em Plasma , Poluentes Químicos da Água , Poluentes da Água , Catálise , Escherichia coli , Grafite , Óxidos/química , Prata/química , Staphylococcus aureus , Titânio , Água , Poluentes Químicos da Água/química
20.
Int J Mol Sci ; 23(11)2022 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-35683009

RESUMO

Due to their potential benefits, cold atmospheric plasmas (CAPs), as biotechnological tools, have been used for various purposes, especially in medical and agricultural applications. The main effect of CAP is associated with reactive oxygen and nitrogen species (RONS). In order to deliver these RONS to the target, direct or indirect treatment approaches have been employed. The indirect method is put into practice via plasma-activated water (PAW). Despite many studies being available in the field, the permeation mechanisms of RONS into water at the molecular level still remain elusive. Here, we performed molecular dynamics simulations to study the permeation of RONS from vacuum into the water interface and bulk. The calculated free energy profiles unravel the most favourable accumulation positions of RONS. Our results, therefore, provide fundamental insights into PAW and RONS chemistry to increase the efficiency of PAW in biological applications.


Assuntos
Gases em Plasma , Água , Gases em Plasma/química , Espécies Reativas de Nitrogênio/química , Espécies Reativas de Oxigênio/química , Vácuo , Água/química
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