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1.
Ecotoxicol Environ Saf ; 240: 113703, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35659700

RESUMO

Atmospheric pressure cold plasma (APCP) holds great potential as an efficient, economical and eco-friendly approach for improving crop production. Although APCP-induced plant growth promotion is undisputedly attributed to the reactive oxygen and nitrogen species (RONS), how these RONS regulate the intracellular redox state and plant growth is still largely unknown. This study systematically investigates the regulation mechanism of APCP-generated RONS on intracellular redox homeostasis in Arabidopsis thaliana seedling by measuring the RONS compositions in APCP-treated solutions and intracellular RONS and antioxidants in Arabidopsis seedlings. The results show that APCP exhibited a dual effect (stimulation or inhibition) on Arabidopsis seedling growth dependent on the treatment time. APCP-generated RONS in liquids increased in a time-dependent manner, leading to an increase of conductivity and oxidation reduction potential (ORP) and decrease of pH. APCP caused an enrichment of intracellular RONS and most of them increased with APCP treatment time. Meanwhile, APCP treatment accelerated malondialdehyde (MDA) generation, and the level of intracellular antioxidants were enhanced by low-dose APCP treatment while decreased at high doses. The results of correlation analysis showed that the extracellular RONS produced by APCP were positively correlated with the intracellular RONS and negatively correlated with the antioxidants. These results demonstrate that the improved antioxidant capacity induced by moderate APCP-generated RONS plays an important role in the growth promotion of Arabidopsis seedlings, which may be a promising alternative for fertilizers in agricultural production.


Assuntos
Arabidopsis , Gases em Plasma , Antioxidantes/metabolismo , Arabidopsis/metabolismo , Pressão Atmosférica , Homeostase , Nitrogênio/química , Oxirredução , Oxigênio , Gases em Plasma/farmacologia , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio , Plântula/metabolismo
2.
Front Immunol ; 13: 868386, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35720416

RESUMO

Skin diseases are mainly divided into infectious diseases, non-infectious inflammatory diseases, cancers, and wounds. The pathogenesis might include microbial infections, autoimmune responses, aberrant cellular proliferation or differentiation, and the overproduction of inflammatory factors. The traditional therapies for skin diseases, such as oral or topical drugs, have still been unsatisfactory, partly due to systematic side effects and reappearance. Cold atmospheric plasma (CAP), as an innovative and non-invasive therapeutic approach, has demonstrated its safe and effective functions in dermatology. With its generation of reactive oxygen species and reactive nitrogen species, CAP exhibits significant efficacies in inhibiting bacterial, viral, and fungal infections, facilitating wound healing, restraining the proliferation of cancers, and ameliorating psoriatic or vitiligous lesions. This review summarizes recent advances in CAP therapies for various skin diseases and implicates future strategies for increasing effectiveness or broadening clinical indications.


Assuntos
Gases em Plasma , Dermatopatias , Humanos , Nitrogênio , Oxigênio , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio , Dermatopatias/tratamento farmacológico
3.
Sci Rep ; 12(1): 9481, 2022 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-35676321

RESUMO

Trichophyton rubrum, a major human pathogenic dermatophyte, is responsible for the most recurrent dermatophytoses as globally important superficial fungal infections. Typical chemotherapy is used to handle such infections; however, emerging drug resistance and side effects necessitate the new remedial method development. Cold atmospheric plasma (CAP) is an emerging technology, consisted of neutral and charged particles and photons newly developed as a potent and safe antimicrobial technique to combat drug-resistant microbial pathogens. In the present study, the vast effects of CAP irradiation containing oxygen (2%) and helium (98%) on T. rubrum growth and pathogenicity were explored. After exposure of T. rubrum to CAP jet for 90, 120, 150, 180, and 210 s in 96-well microtiter plates, cell morphology and viability, ergosterol content of fungal hyphae, HSP90 gene expression, and the pattern of drug susceptibility were studied by using electron microscopy, RT-qPCR, spectrophotometry, disk diffusion and CLSI microbroth dilution methods. CAP irradiation significantly inhibited the fungal growth by 25.83 to 89.10%, reduced fungal cell viability by 11.68 to 87.71%, disrupted cellular membranous organelles and structures of the fungal hyphae, and suppressed efficiently the expression of HSP90 gene by 2 folds in 210 s exposure. Taken together, our results demonstrated that CAP is an efficient tool with potential in-vivo therapeutic applications against chronic dermatophytosis caused by T. rubrum due to its effectiveness, harmless, and ease of access.


Assuntos
Antifúngicos , Arthrodermataceae , Gases em Plasma , Antifúngicos/farmacologia , Arthrodermataceae/efeitos dos fármacos , Arthrodermataceae/crescimento & desenvolvimento , Expressão Gênica , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/metabolismo , Gases em Plasma/farmacologia
4.
Int J Biol Sci ; 18(8): 3405-3420, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35637956

RESUMO

Objectives: Vaccination still remains as the most effective approach for preventing infectious diseases such as those caused by virus infection, with cell-based vaccine manufacturing being one flexible solution regarding the spectrum of infectious disorders it can prevent. Rapid cell-based virus propagation can enable high yield of vaccines against viral diseases that may offer critical values in the industry when handling emergent situations such as the ongoing viral disease pandemic. Methods: Through investigating the phenomenon and biological mechanism underlying redox-triggered cell survival towards enhanced viral particle production, this study explores novel strategies for improved yield of viral particles at a reduced cost to meet the increasing demand on cell-based vaccine manufacturing against viral diseases. Results: We found in this study that cold atmospheric plasma (CAP), composed of multiple reactive oxygen and nitrogen species including H2O2, could effectively enhance virus replication via triggering cell mitophagy that was dynamically modulated by the p-EGFR(Tyr1068)/p-Drp1(Ser616) axis using IBRV and MDBK as the virus and cell models, respectively; and removing H2O2 can further enhance virus yield via releasing cells from excessive G0/G1 cell cycle arrest. The observed efficacy of CAP was extended to other viruses such as CDV and CPV. Conclusion: This study provides experimental evidences supporting the use of CAP as a modulator of cell survival including mitophagy and mitochondria dynamics, and makes CAP an interesting and promising tool for enhancing the yield of viral vaccines if translated into the industry.


Assuntos
Gases em Plasma , Viroses , Receptores ErbB , Humanos , Peróxido de Hidrogênio , Mitofagia , Fosforilação , Gases em Plasma/farmacologia , Replicação Viral
5.
Int J Food Microbiol ; 375: 109738, 2022 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-35635991

RESUMO

Fresh tilapia fillets are susceptible to perish due to the microbial contamination during storage. High voltage atmospheric cold plasma (HVACP), a non-thermal technology, can effectively inactivate various microorganism. The aim of this study was to identify the microorganism amount and diversity changes of fresh tilapia fillets during refrigerator storage after HVACP treatment. Samples were treated at 70 kV for 1, 3 and 5 min by dielectric discharge barrier (DBD) cold plasma then stored at 4 °C. During the storage, amounts of Total viable bacteria (TVB), Psychrophilic bacteria, Pseudomonas spp., Lactic acid bacteria, Enterobacteriaceae, H2S-producing bacteria were measured, and microbial diversity of samples was analyzed. Long treatment time showed a great reduction effect on amounts of all bacteria. When tilapia fillets were treated at 70 kV for 5 min and stored for 12 d, amounts of TVB, Pseudomonas spp. and Enterobacteriaceae were 7.15, 6.99 and 4.23 log CFU/g, respectively, which were significantly lower (P < 0.05) than those in control group. High-throughput sequencing results showed that microbial diversity of tilapia fillets treated by HVACP was fluctuated as storage time extend, microbial species richness was decreased during first two days, and increased to the peak till 9 d, then decreased again. The dominant bacteria in fresh samples were Acinetobacter, Macrococcus, Pseudomonas, and Lactococcus. The abundance of both Acinetobacter and Macrococcus were decreased gradually during storage, while the abundance of Lactococcus was increased at first 3 d then decreased. After 12 d of storage, the dominant bacteria were transformed into Pseudomonas, Arthrobacter, and Kurthia.


Assuntos
Gases em Plasma , Tilápia , Animais , Bactérias/genética , Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Gases em Plasma/farmacologia , Refrigeração , Tilápia/microbiologia
6.
Sci Rep ; 12(1): 7597, 2022 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-35534525

RESUMO

To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 105 colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future.


Assuntos
Anti-Infecciosos , Ozônio , Gases em Plasma , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Peróxido de Hidrogênio/farmacologia , Ozônio/farmacologia , Gases em Plasma/farmacologia
7.
Arch Microbiol ; 204(6): 343, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-35596084

RESUMO

Cold atmospheric plasma (CAP) is being used recently as a modern technique for microbial random mutagenesis. In the present study, CAP was used to induce mutagenesis in L. enzymogenes which is the bacteria known for producing proteolytic enzymes especially lysyl endopeptidase (Lys C). Enhanced proteolytic activity was the main criteria to select mutant strains. Therefore, the cell suspension of L. enzymogenes strain (ATCC 29487), was exposed to CAP for 30, 45, 90, and 150 s. The proteolytic activity of mutant strains was screened initially by radial caseinolytic assay and then by Ansons method in different phases of bacterial growth in the selected mutants. The purification process of Lysyl endopeptidase as the target enzyme was optimized and for enlightening molecular aspect of CAP mutagenesis, the sequences of the upstream and coding regions of lys C gene from 10 selected mutant strains were determined. The bacterial survival assessment showed that the more CAP treatment time, the less survival rate, however, in all exposure times, a number of survived mutants showed enhanced proteolytic activity. Among 38 out of 100 examined mutants which showed higher proteolytic activity than that of wild type, the M1-30 s mutant exhibited the highest increment to 1.94 fold. The SDS-PAGE analysis showed expected size of purified Lys C from M1-30 s. The Lys C gene from M14-150 s mutant strain (1.4-fold increment) harbored three point mutations which can be effective in enhancing protease activity. In conclusion, the results highlighted the role of CAP for strain improvement process to obtain industrial strains.


Assuntos
Lysobacter , Gases em Plasma , Bactérias/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Lysobacter/genética , Lysobacter/metabolismo , Gases em Plasma/metabolismo , Gases em Plasma/farmacologia
8.
Biointerphases ; 17(2): 021004, 2022 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-35360909

RESUMO

The possible benefits of an atmospheric pressure plasma jet skin treatment have been tested in vivo on mouse skin. Many studies have been conducted in vitro on mouse skin cells, but only a few in vivo where, due to the complexity of the biological system, plasma can cause severe damages. For this reason, we investigated how kHz plasma generated in a jet that is known to inflict skin damage interacts with mouse skin and explored how we can reduce the skin damage. First, the focus was on exploring plasma effects on skin damage formation with different plasma gases and jet inclinations. The results pointed to the perpendicular orientation of a He plasma jet as the most promising condition with the least skin damage. Then, the skin damage caused by a He plasma jet was explored, focusing on damage mitigation with different liquid interfaces applied to the treatment site, adding N2 to the gas mixture, or alternating the gas flow dynamics by elongating the jet's glass orifice with a funnel. All these mitigations proved highly efficient, but the utmost benefits for skin damage reduction were connected to skin temperature reduction, the reduction in reactive oxygen species (ROS), and the increase in reactive nitrogen species (RNS).


Assuntos
Pressão Atmosférica , Gases em Plasma , Animais , Gases , Camundongos , Gases em Plasma/farmacologia , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio
9.
Food Microbiol ; 105: 104007, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35473970

RESUMO

Listeria (L.) monocytogenes is a significant pathogen found in ready-to-eat meat and dairy products. Soft cheeses, such as Queso Fresco cheese (QFC), are particularly sensitive to Listeria contamination, and occasionally serve as a source of food-borne illness outbreaks. In the present study, clinical and cheese isolates of L. monocytogenes were assayed for phenotypic characteristics following sub-lethal high voltage atmospheric cold plasma (HVACP) treatment. Reductions in biofilm formation, swimming motility, and growth dynamics were observed following HVACP treatment. Microbial enumeration of 1-, 10-, and 100-g fresh QFC following 0, 1, 2, or 3 min of HVACP demonstrated significant reductions in L. monocytogenes after 1 min (P-value <0.05), with increasing efficacy with prolonged exposure. A mass-dependent effect was observed between treatments of 1-, 10-, and 100-g QFC in regard to treatment efficacy. This result indicates that greater L. monocytogenes reduction on a larger QFC mass requires greater exposure of the L. monocytogenes to the reactive gas species. Optical absorption spectroscopy confirmed a reduction in reactive gas species for each log increase in QFC mass, however, an equivalent volume of inert foam resulted in increased reactive gas generation compared to QFC. In conclusion, we demonstrate both the application and limitations of HVACP treatments of QFC in the currently defined experimental parameters.


Assuntos
Queijo , Listeria monocytogenes , Listeria , Gases em Plasma , Microbiologia de Alimentos , Gases em Plasma/farmacologia
10.
Theranostics ; 12(6): 2811-2832, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35401827

RESUMO

Rational: The mutating SARS-CoV-2 potentially impairs the efficacy of current vaccines or antibody-based treatments. Broad-spectrum and rapid anti-virus methods feasible for regular epidemic prevention against COVID-19 or alike are urgently called for. Methods: Using SARS-CoV-2 virus and bioengineered pseudoviruses carrying ACE2-binding spike protein domains, we examined the efficacy of cold atmospheric plasma (CAP) on virus entry prevention. Results: We found that CAP could effectively inhibit the entry of virus into cells. Direct CAP or CAP-activated medium (PAM) triggered rapid internalization and nuclear translocation of the virus receptor, ACE2, which began to return after 5 hours and was fully recovered by 12 hours. This was seen in vitro with both VERO-E6 cells and human mammary epithelial MCF10A cells, and in vivo. Hydroxyl radical (·OH) and species derived from its interactions with other species were found to be the most effective CAP components for triggering ACE2 nucleus translocation. The ERα/STAT3(Tyr705) and EGFR(Tyr1068/1086)/STAT3(Tyr705) axes were found to interact and collectively mediate the effects on ACE2 localization and expression. Conclusions: Our data support the use of PAM in helping control SARS-CoV-2 if developed into products for nose/mouth spray; an approach extendable to other viruses utilizing ACE2 for host entry.


Assuntos
COVID-19 , Gases em Plasma , Enzima de Conversão de Angiotensina 2 , COVID-19/prevenção & controle , Humanos , Gases em Plasma/farmacologia , Ligação Proteica , SARS-CoV-2 , Glicoproteína da Espícula de Coronavírus/metabolismo
11.
Food Chem ; 388: 133064, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-35486991

RESUMO

Buckwheat (BW) is rich in γ-aminobutyric acid (GABA), which has great potential as a functional anti-hypertensive food ingredient. This study utilized cold atmospheric plasma (CAP) to promote GABA accumulation in BW during germination. The effect of this approach on GABA enrichment and anti-hypertensive activity of BW along with its processing properties were investigated. The results indicated that CAP stress treatment (50 W for 40 s) of BW seeds followed by germination at 30 °C for 60 h resulted in a GABA content of 2.22 ± 0.06 mg/g, which was a 2.64-fold greater than that of pristine BW. Cracking of the seed coat, faster germination, and the activation of glutamate decarboxylase might be responsible for the GABA enrichment. Compared with pristine BW, the GABA-enriched BW powder showed a greater inhibitory effect on the angiotensin-converting enzyme, an increased degree of pre-gelatinization and elasticity when it was formed into a dough.


Assuntos
Fagopyrum , Gases em Plasma , Anti-Hipertensivos/farmacologia , Germinação , Gases em Plasma/farmacologia , Sementes , Ácido gama-Aminobutírico/farmacologia
12.
Sci Rep ; 12(1): 6270, 2022 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-35428374

RESUMO

Plasma therapy offers an exciting and novel way of cancer treatment. Specifically, it is shown that Jurkat death rates are closely governed by the plasma treatment time. However, apart from time, alterations to different parameters of treatment process may yield better results. Here, Dielectric barrier discharge (DBD) reactors excited by a nanosecond-pulse energy source are used to investigate cell viability for longer exposure times as well as the effects of polarity of reactor on treatment. Plasma discharge regimes are discussed and assessed using imaging and thermal imaging methods. We found that by changing the polarity of reactor i.e. changing the direction of plasma discharge, the plasma discharge regime changes influencing directly the effectiveness of treatment. Our results showed that ns-DBD- reactor could induce both apoptosis and necrosis of human Jurkat and U937 cells, and this cytotoxic effect of plasma was not completely antagonized by N-acetyl cysteine. It indicates that plasma could induce ROS-independent cell death. Gene expression analyses revealed that p53, BAD, BID and caspase 9 may play vital roles in plasma caused cell death. In addition, our findings demonstrate how different parameters can influence the effectiveness of our reactors. Our assay reveals the custom ability nature of plasma reactors for hematologic cancer therapy and our findings can be used for further development of such reactors using multi-objective optimisation techniques.


Assuntos
Leucemia , Gases em Plasma , Apoptose , Humanos , Células Jurkat , Gases em Plasma/farmacologia , Células U937
13.
Food Microbiol ; 104: 103976, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35287805

RESUMO

Salmonella, a foodborne pathogen, has been frequently associated with recalls of fresh food products, including poultry meat products. Atmospheric cold plasma (ACP) is a novel non-thermal technology, which has potential to reduce pathogens in food products. This study demonstrates the synergistic interactions of food grade organic acids (i.e., lactic acid (LA) or gallic acid (GA)) and ACP to inactivate Salmonella enterica Typhimurium ATCC 13311 inoculated on polycarbonate membrane filter paper and poultry meat surface. Organic acids were used in the form of a spray to enhance dispersion on samples surface. The sequential treatment of organic acid followed by ACP synergistically reduced S. Typhimurium on poultry meat surface. Irrespective of the type of organic acid, an average reduction of more than 3.5 log CFU/cm2 in S. Typhimurium on filter paper was obtained, when a combination of 10 mM LA or GA with an ACP exposure of 30 s was tested. However, the individual treatments of LA, GA, and ACP resulted in only 0.4, 0.3, 1.2 log CFU/cm2 reduction in S. Typhimurium, respectively. On poultry meat surface, a higher level of organic acid concentration (i.e., 50 mM) in combination with 30 s ACP was required to achieve more than 2.5 log CFU/cm2 reduction in S. Typhimurium. Our investigation on inactivation mechanisms revealed that the sequential treatment of LA or GA with ACP resulted in a significantly higher level of membrane permeability and membrane lipid peroxidation in S. Typhimurium cells. Additionally, the combined treatment significantly reduced the cell metabolic activity and affected the intracellular reactive oxygen species level of S. Typhimurium. In summary, this study demonstrated the potential synergistic benefits of combining organic acids and ACP to achieve a higher level of bacterial inactivation.


Assuntos
Gases em Plasma , Salmonella typhimurium , Ácidos , Bactérias , Ácido Láctico , Gases em Plasma/farmacologia
14.
Ultrason Sonochem ; 84: 105960, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35240411

RESUMO

Ultrasound (US) has limited disinfection efficacy, and it has been recommended to combine it with chemical disinfectants during fresh produce washing. After washing and before packaging, the disinfection effect of US-assisted washing can be weakened; thus, in-package disinfection is important. As a nutritious fruit, there are no packaged blueberries can be directly eaten. Therefore, in this study, blueberry was selected as the model, and the two most commonly used disinfectants (free chlorine [FC] at 10 ppm and peracetic acid [PAA] at 80 ppm) were combined with low-frequency US (25 kHz) during washing, followed by in-package disinfection using dielectric barrier discharge cold plasma (CP). The disinfection efficacy of US-FC and US-PAA against Escherichia coli O157:H7 and Salmonella Typhimurium was significantly higher than that of US, PAA, or FC alone. The highest disinfection efficacy of CP was observed at the pulse frequency range of 400-800 Hz. For US-FC (1 min) + CP (1 min), an additional 0.86, 0.71, 0.42, and 0.29 log CFU/g of reduction for E. coli O157:H7, S. Typhimurium, aerobic mesophilic counts, and mold and yeast was achieved, respectively, compared with US-FC (2 min) alone. For US-PAA (1 min) + CP (1 min) an additional 0.71, 0.59, 0.32, and 0.21 log CFU/g of reduction was achieved for the above organisms, respectively, compared with US-PAA (2 min) alone. Quality loss (in total color difference, firmness, and anthocyanin content) was not observed after treatment with US-FC + CP, US-PAA + CP, US-FC, or US-PAA. After treatment with US-FC + CP or US-PAA + CP, the reactive oxygen species (ROS) content was significantly lower than that in the other groups, and antioxidant enzyme activity was significantly higher than that in the other groups, suggesting that in-package CP can activate the blueberry antioxidant system to scavenge ROS, thereby lowering the risk of quality loss. US-CP combination not only improves the disinfection efficacy but also lowers quality loss caused by ROS, without prolonging the processing time.


Assuntos
Mirtilos Azuis (Planta) , Desinfetantes , Escherichia coli O157 , Gases em Plasma , Contagem de Colônia Microbiana , Desinfetantes/farmacologia , Desinfecção , Manipulação de Alimentos , Microbiologia de Alimentos , Gases em Plasma/farmacologia , Tecnologia
15.
J Biophotonics ; 15(6): e202100403, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35261164

RESUMO

Cold atmospheric plasma (CAP) has attracted increasing attention due to its anti-bacterial and anti-tumor effects. Melanoma is an aggressive malignancy with increasing incidence rate and poor prognosis. Evaluating cell viability, apoptosis rate and reactive species injection efficiency of melanoma cells and human keratinocyte cells (HaCaT) treated with CAP to analyze biological safety of CAP. RNA-sequencing (RNA-seq) of A875 cells before and after treatment was performed to further explore the anti-tumor mechanism of CAP. CAP had a more significant biological effect on melanoma cells than HaCaT cells by inhibiting proliferation and promoting apoptosis. RNA-sequencing analysis showed that besides MAPK and p53 apoptotic signaling pathways, necroptosis and autophagy also played important roles in CAP-induced melanoma cells death. CAP can selectively kill melanoma cells and has good biosafety cytologically. Besides apoptosis, CAP can induce cell death via autophagy and necroptosis.


Assuntos
Melanoma , Gases em Plasma , Apoptose/genética , Linhagem Celular Tumoral , Contenção de Riscos Biológicos , Humanos , Melanoma/genética , Melanoma/patologia , Gases em Plasma/farmacologia , RNA/farmacologia
16.
ACS Appl Bio Mater ; 5(4): 1610-1623, 2022 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-35324138

RESUMO

Glioblastoma (GBM) is a fatal human brain tumor with a low survival rate. Temozolomide (TMZ) has been widely used in GBM therapy with noticeable side effects. Cold plasma is an ionized gas that is generated near room temperature. Here, we demonstrated the enhancement therapeutic efficacy of TMZ via using a cold plasma source based on nonequilibrium plasma in a sealed glass tube, named a radial cold plasma discharge tube (PDT). The PDT affected glioblastoma cells' function just by its electromagnetic (EM) emission rather than any chemical factors in the plasma. The PDT selectively increased the cytotoxicity of TMZ on two typical glioblastoma cell lines, U87MG and A172, compared with normal astrocyte cell line hTERT/E6/E7 to some extent. Furthermore, on the basis of a patient-derived xenograft model, our preliminary in vivo studies demonstrated the drastically improved mean survival days of the tumor-barrier mice by more than 100% compared to control. The PDT is not only independent of continuous helium supply but is also capable of resisting the interference of environmental changes. Thus, the PDT was a stable and low-cost cold atmospheric plasma source. In short, this study is the first to demonstrate the promising application of PDTs in GBM therapy as a noninvasive and portable modality.


Assuntos
Glioblastoma , Gases em Plasma , Animais , Linhagem Celular Tumoral , Glioblastoma/tratamento farmacológico , Humanos , Camundongos , Gases em Plasma/farmacologia , Temozolomida/farmacologia , Ensaios Antitumorais Modelo de Xenoenxerto
17.
Int J Implant Dent ; 8(1): 12, 2022 03 11.
Artigo em Inglês | MEDLINE | ID: mdl-35275307

RESUMO

BACKGROUND: The aim of the current study was to comparatively assess the efficiency of three different adjunctive therapy options (cold atmospheric plasma, [CAP], photodynamic therapy [PDT] and chemical decontamination via 35% phosphoric acid gel [PAG]) on decontamination of titanium implant surfaces in-vitro. MATERIALS AND METHODS: Implants were inserted in concavities of four mm in depth mimicking a bone defect at the implant recipient site. In each model, two implants were inserted in the fourth and one implant in the third quadrants. After contamination with E. faecalis, the first group has been treated with CAP for 3 min, the second group with 35% PAG (and the third group with PDT. After treatment, quantification of bacterial colonization was assessed by quantification via colony forming units and qualitatively by fluorescence microscopy and scanning electron microscopy. RESULTS: With a mean value of 1.24 × 105 CFU/ml, the CAP treated implants have showed the least microorganisms. The highest number of CFU was found after PDT with mean value of 8.28 × 106 CFU/ml. For the implants that were processed with phosphoric acid, a mean value of 3.14 × 106 CFU/ml could be detected. When the groups were compared, only the CAP and PDT groups differed significantly from each other (p = 0.005). CONCLUSION: A complete cleaning of the micro-textured implant surface or the killing of the bacteria could not be achieved by any of the investigated treatment options, thus bacteria in the microstructure of the titanium surface cannot be completely reached by mechanical and physico-chemical processes. CLINICAL RELEVANCE: The main goal of the adjunctive peri-implantitis treatment is the decontamination of the implant surface. However, there is still an ongoing need to define the most appropriate adjunctive therapy method. Due to its antimicrobial effects, CAP combined with mechanical debridement could be a feasible treatment modality in the management of peri-implantitis.


Assuntos
Implantes Dentários , Peri-Implantite , Gases em Plasma , Descontaminação , Implantes Dentários/microbiologia , Humanos , Peri-Implantite/prevenção & controle , Gases em Plasma/farmacologia , Titânio/farmacologia
18.
Int J Food Microbiol ; 369: 109626, 2022 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-35303494

RESUMO

Consecutive treatment with intense pulsed light (IPL) and cold plasma (CP) was evaluated as an intervention technology to decontaminate cabbage slices in plastic containers. Salmonella inactivation efficacy increased as the treatment time and voltage of IPL and CP treatments increased. The rotation of the container during IPL treatment as well as the selected sequence of treatment, CP treatment followed by IPL treatment (CP-IPL), were beneficial in inactivating Salmonella. Under the determined conditions (CP: 24.5 kV, 2 min; IPL: 1.5 kV, 2 min), the inactivation levels of Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and Bacillus cereus spores using CP-IPL treatment were 3.1, 2.9, 3.2 log CFU/g, and 2.1 log spores/g, respectively. The efficacy of Salmonella inactivation in cabbage slices using CP-IPL treatment was maintained during storage at 4 °C and 10 °C. CP-IPL treatment did not alter the color and lipid peroxidation of cabbage slices. At identical treatment times, CP-IPL treatment alleviated the antioxidant activity decrease and the damage to the cabbage cell membrane compared with CP treatment alone, while resulting in a higher microbial inactivation efficacy. This study demonstrates that CP-IPL treatment has the potential to effectively increase the microbiological safety of cabbage slices in plastic containers with minimal impact on their quality.


Assuntos
Brassica , Listeria monocytogenes , Gases em Plasma , Contagem de Colônia Microbiana , Descontaminação/métodos , Microbiologia de Alimentos , Gases em Plasma/farmacologia , Plásticos
19.
Sci Rep ; 12(1): 3646, 2022 03 07.
Artigo em Inglês | MEDLINE | ID: mdl-35256655

RESUMO

Accurate and timely diagnosis of appendicitis in children can be challenging, which leads to delayed admittance or misdiagnosis that may cause perforation. Surgical management involves the elimination of the focus (appendectomy) and the reduction of the contamination with peritoneal irrigation to prevent sepsis. However, the validity of conventional irrigation methods is being debated, and novel methods are needed. In the present study, the use of cold plasma treated saline solution as an intraperitoneal irrigation solution for the management of acute peritonitis was investigated. Chemical and in vitro microbiological assessments of the plasma-treated solution were performed to determine the appropriate plasma treatment time to be used in in-vivo experiments. To induce acute peritonitis in rats, the cecal ligation and perforation (CLP) model was used. Sixty rats were divided into six groups, namely, sham operation, plasma irrigation, CLP, dry cleaning after CLP, saline irrigation after CLP, and plasma-treated saline irrigation after CLP group. The total antioxidant and oxidant status, oxidative stress index, microbiological, and pathological evaluations were performed. Findings indicated that plasma-treated saline contains reactive species, and irrigation with plasma-treated saline can effectively inactivate intraperitoneal contamination and prevent sepsis with no short-term local and/or systemic toxicity.


Assuntos
Peritonite , Gases em Plasma , Sepse , Animais , Modelos Animais de Doenças , Cavidade Peritoneal/microbiologia , Lavagem Peritoneal/métodos , Peritonite/etiologia , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Ratos , Solução Salina , Sepse/complicações
20.
Bull Exp Biol Med ; 172(5): 570-572, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35352246

RESUMO

We studied the effect of course exposure to argon cold plasma (ten 1- and 2-min procedures) on some parameters of the oxidative metabolism of rat blood plasma. The intensity of free radical processes, the total antioxidant activity, and malondialdehyde concentration in rat plasma were evaluated. It was found that 2-min exposure to argon cold plasma and nonionized argon stream produce a prooxidant effect, while 1-min exposure argon plasma led to stimulation of the antioxidant reserves of the blood.


Assuntos
Gases em Plasma , Animais , Antioxidantes/farmacologia , Argônio/farmacologia , Malondialdeído , Estresse Oxidativo , Gases em Plasma/farmacologia , Ratos
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