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1.
PLoS One ; 15(1): e0228325, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31999773

RESUMO

Structural knowledge of gastropod hemocyanins is scarce. To better understand their evolution and diversity we studied the hemocyanin of a caenogastropod, Pomacea canaliculata (PcH). Through a proteomic and genomic approach, we identified 4 PcH subunit isoforms, in contrast with other gastropods that usually have 2 or 3. Each isoform has the typical Keyhole limpet-type hemocyanin architecture, comprising a string of eight globular functional units (FUs). Correspondingly, genes are organized in eight FUs coding regions. All FUs in the 4 genes are encoded by more than one exon, a feature not found in non- caenogastropods. Transmission electron microscopy images of PcH showed a cylindrical structure organized in di, tri and tetra-decamers with an internal collar structure, being the di and tri-decameric cylinders the most abundant ones. PcH is N-glycosylated with high mannose and hybrid-type structures, and complex-type N-linked glycans, with absence of sialic acid. Terminal ß-N-GlcNAc residues and nonreducing terminal α-GalNAc are also present. The molecule lacks O-linked glycosylation but presents the T-antigen (Gal-ß1,3-GalNAc). Using an anti-PcH polyclonal antibody, no cross-immunoreactivity was observed against other gastropod hemocyanins, highlighting the presence of clade-specific structural differences among gastropod hemocyanins. This is, to the best of our knowledge, the first gene structure study of a Caenogastropoda hemocyanin.


Assuntos
Gastrópodes/genética , Gastrópodes/metabolismo , Hemocianinas/química , Hemocianinas/genética , Animais , Evolução Molecular , Gastrópodes/química , Perfilação da Expressão Gênica , Genômica , Hemocianinas/metabolismo , Espectrometria de Massas , Microscopia Eletrônica de Transmissão , Modelos Moleculares , Conformação Proteica , Domínios Proteicos , Isoformas de Proteínas/química , Isoformas de Proteínas/metabolismo , Proteômica
2.
Chem Commun (Camb) ; 56(10): 1605-1607, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31939465

RESUMO

We report the first preparation of a monoclonal antibody (mAb) that can immobilize a palladium (Pd)-complex. The allylic amination reaction using a supramolecular catalyst consisting of the Pd-complex and mAb selectively gives the (R)-enantiomer product with an enantiomeric excess (ee) of 98 ± 2%. This is in sharp contrast to the reaction catalyzed by a conventional Pd-catalyst (ee < 2%).


Assuntos
Anticorpos Monoclonais/química , Complexos de Coordenação/química , Paládio/química , Compostos Alílicos/síntese química , Compostos Alílicos/química , Aminação , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/metabolismo , Benzilaminas/síntese química , Benzilaminas/química , Catálise , Bovinos , Complexos de Coordenação/imunologia , Complexos de Coordenação/metabolismo , Reações Cruzadas/imunologia , Feminino , Gastrópodes/química , Hemocianinas/química , Camundongos Endogâmicos BALB C , Ligação Proteica , Ródio/química , Soroalbumina Bovina/química , Estereoisomerismo , Água/química
3.
Arch Environ Contam Toxicol ; 78(3): 337-349, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31938850

RESUMO

Occurrence of traditional (PBDEs) and novel (HBB, PBEB, DBDPE) brominated flame retardants, as well as the natural compounds of MeO-PBDEs, were studied in a shellfish species (Hexaplex trunculus) sampled from Bizerte Lagoon. PBDE and MeO-PBDE mean concentrations in murex soft tissues were 187 and 264 ng g-1 lw respectively. The alternative flame retardants were not identified. The sum of PBDE and MeO-PBDE levels recorded in murex from the investigated aquatic ecosystem were comparable or a relatively lower than those reported for other organisms from other regions across the world. The amount of PBDE and MeO-PBDE concentrations from the Bizerte Lagoon recorded in murex were comparable or a relatively lower than those recorded from other areas across the world for other species. There is not a danger to the population health with regard to PBDE intakes associated with the consumption of murex in Bizerte city. We believe that this is the first study of the analysis of these pollutants in marine gastropod mollusks from Tunisian aquatic areas.


Assuntos
Monitoramento Ambiental/métodos , Retardadores de Chama/análise , Gastrópodes/química , Éteres Difenil Halogenados/análise , Hidrocarbonetos Bromados/análise , Poluentes Químicos da Água/análise , Animais , Ecossistema , Humanos , Medição de Risco , Frutos do Mar/análise , Tunísia
4.
Chem Biodivers ; 17(1): e1900560, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31769919

RESUMO

Borrelidins M-O (1-3), along with four previously known family members (4-7), were isolated from marine pulmonated mollusks Onchidium sp. associated Streptomyces olivaceus SCSIO LO13. The structures of 1-3 were elucidated by extensive spectral analyses of HR-ESI-MS, 1D and 2D NMR data. In addition, the cytotoxic and antibacterial activities of 1-7 were evaluated enabling us to propose some tentative structure-activity relationships (SARs), especially those involving modifications at C(22) and the moieties at C(7) and C(8) of the borrelidin scaffold.


Assuntos
Antibacterianos/farmacologia , Antineoplásicos/farmacologia , Gastrópodes/química , Streptomyces/química , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Álcoois Graxos/química , Álcoois Graxos/isolamento & purificação , Álcoois Graxos/farmacologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Relação Estrutura-Atividade
5.
Food Funct ; 10(10): 6644-6654, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31556417

RESUMO

Sulfated polysaccharides (CCPS) obtained from Cipangopaludina chinensis have a certain protective effect on cardiovascular diseases, but whether they can slow down the development of atherosclerosis (AS) and how they work are still unclear. In this study, we aimed to determine the preventive effects of CCPS on high-fat-diet (HFD)-induced AS in mice and the underlying mechanism focusing on gut microbiota profile modulation. First, male apolipoprotein E knockout mice were fed HFD or normal diet and simultaneously intervened by a blank solvent, CCPS or atorvastatin to last for 12 weeks. Then, the histopathological characteristics, biochemical parameters and gut microbiota compositions of the experimental groups were compared. The results demonstrated that the CCPS supplementation significantly improved HFD-induced AS of the mice, which was mainly manifested by regulating the plasma lipid balance, decreasing the atherosclerotic index and reducing the atherosclerotic plaque area in a dose-dependent manner. Mechanistic analysis revealed that CCPS regulated the bacterial composition including reducing the ratio of Firmicutes/Bacteroidetes, decreasing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria. Furthermore, through environmental factor correlation analysis, we found that the preventive effects of CCPS on AS in mice induced by HFD were closely related to reshaping the composition of gut microbiota.


Assuntos
Aterosclerose/prevenção & controle , Microbioma Gastrointestinal/efeitos dos fármacos , Gastrópodes/química , Polissacarídeos/administração & dosagem , Substâncias Protetoras/administração & dosagem , Animais , Apolipoproteínas E/deficiência , Apolipoproteínas E/genética , Aterosclerose/genética , Aterosclerose/metabolismo , Aterosclerose/microbiologia , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Dieta Hiperlipídica/efeitos adversos , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Polissacarídeos/química , Substâncias Protetoras/química
6.
Aquat Toxicol ; 215: 105284, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31479758

RESUMO

Metal pollution in the environment is a serious threat to the biological sustainability of coastal ecosystems. However, our current understanding of the biological effects of metals in these ecosystems is limited. Herein, we investigated the responses of the sea slug Onchidium reevesii to persistent sublethal Cd environmental stress. Dynamic expression was analyzed using various biomarkers. The full-length cDNA of O. reevesii metallothionein (MT) was cloned and consists of 1639 nucleotides encoding a 65 amino acid polypeptide. Phylogenetic analysis showed that Or-MT has conserved Cys residues typical of MTs, including a typical Cys-X-Cys motif, implying that it can function the same as the MT of other shellfish. Expression of Or-MT in response to Cd varied in different tissues, and was highest in gastropod tissues. Thus, regiotemporal expression of MT may be useful for assessing pollution in coastal areas. Cellular immunity (in the hemolymph) and enzyme activity (in the hepatopancreas) were investigated along with hemocyte viability, hemocyte phagocytosis, and superoxide dismutase (SOD) and aspartate aminotransferase (AST) activities. Hemocyte viability was elevated under continuous Cd exposure but hemocyte phagocytosis was decreased. SOD and AST activities in the hepatopancreas fluctuated considerably, and SOD activity was more sensitive. SOD activity was lowest at 4 h and highest at 12 h, while AST activity peaked at 2 h and was lowest at 48 h. Thus, changes in enzyme activity may reveal adaptation to stress. Furthermore, the response patterns of certain enzymes, cellular immunity, and MT expression in O. reevesii could serve as biomarkers of Cd pollution in aquatic environments.


Assuntos
Aspartato Aminotransferases/metabolismo , Biomarcadores/metabolismo , Cádmio/toxicidade , Gastrópodes/metabolismo , Hemócitos/metabolismo , Metalotioneína/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Sobrevivência Celular/efeitos dos fármacos , Exposição Ambiental , Gastrópodes/química , Gastrópodes/efeitos dos fármacos , Gastrópodes/genética , Hemócitos/efeitos dos fármacos , Fagocitose/efeitos dos fármacos , Filogenia , Homologia de Sequência de Aminoácidos , Poluentes Químicos da Água/toxicidade
7.
Chemosphere ; 236: 124404, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31545201

RESUMO

Tetrodotoxin (TTX) is a potent neurotoxin responsible for countless human intoxications and deaths around the world. The distribution of TTX and its analogues is diverse and the toxin has been detected in organisms from both marine and terrestrial environments. Increasing detections seafood species, such as bivalves and gastropods, has drawn attention to the toxin, reinvigorating scientific interest and regulatory concerns. There have been reports of TTX in 21 species of bivalves and edible gastropods from ten countries since the 1980's. While TTX is structurally dissimilar to saxitoxin (STX), another neurotoxin detected in seafood, it has similar sodium channel blocking action and potency and both neurotoxins have been shown to have additive toxicities. The global regulatory level for the STX group toxins applied to shellfish is 800 µg/kg. The presence of TTX in shellfish is only regulated in one country; The Netherlands, with a regulatory level of 44 µg/kg. Due to the recent interest surrounding TTX in bivalves, the European Food Safety Authority established a panel to assess the risk and regulation of TTX in bivalves, and their final opinion was that a concentration below 44 µg of TTX per kg of shellfish would not result in adverse human effects. In this article, we review current knowledge on worldwide TTX levels in edible gastropods and bivalves over the last four decades, the different methods of detection used, and the current regulatory status. We suggest research needs that will assist with knowledge gaps and ultimately allow development of robust monitoring and management protocols.


Assuntos
Bivalves/química , Contaminação de Alimentos/análise , Gastrópodes/química , Frutos do Mar/análise , Tetrodotoxina/análise , Animais , Contaminação de Alimentos/legislação & jurisprudência , Inocuidade dos Alimentos , Humanos , Países Baixos , Neurotoxinas/análise , Neurotoxinas/farmacocinética , Saxitoxina/análise , Tetrodotoxina/farmacocinética
8.
Molecules ; 24(16)2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31416254

RESUMO

Astragli Radix (AR) is one of the most popular traditional Chinese medicines with chemical constituents including flavonoids and saponins. As recently evidenced, some fungi or their fermentation liquid may have the potential to affect the bioactive constituents and different pharmacological effects of AR. Thus, the composition of fermented AR (FAR) produced by Paecilomyces cicadae (Miquel) Samson in liquid-state fermentation was investigated using a UHPLC-LTQ-Orbitrap mass spectrometer in both positive and negative ion modes. Firstly, the MSn data sets were obtained based on a data-dependent acquisition method and a full scan-parent ions list-dynamic exclusion (FS-PIL-DE) strategy. Then, diagnostic product ions (DPIs) and neutral loss fragments (NLFs) were proposed for better constituent detection and structural characterization. Consequently, 107 constituents in total, particularly microconstituents in FAR and AR, were characterized and compared in parallel on the same LTQ-Orbitrap instrument. Our results indicated that AR fermentation with Paecilomyces significantly influenced the production of saponins and flavonoids, especially increasing the content of astragaloside IV. In conclusion, this research was not only the first to show changes in the chemical components of unfermented AR and FAR, but it also provides a foundation for further studies on the chemical interaction between microbiota and AR.


Assuntos
Medicamentos de Ervas Chinesas/química , Fermentação , Gastrópodes/química , Paecilomyces/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Análise de Dados , Flavonoides/química , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
9.
Adv Exp Med Biol ; 1155: 583-596, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31468433

RESUMO

In this study, we evaluated the protective effects of an aqueous extract from Batillus cornutus meat (BM) against cellular oxidative damage caused by hydrogen peroxide (H2O2) in human hepatocyte, Chang cells. First, we prepared an aqueous extract of BM meat (BMW) showing the highest taurine content among free amino acid contents. BMW led to high antioxidant activity showing 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, good reducing power and an oxygen radical absorbance capacity (ORAC) value. Also, BMW improved cell viability that was diminished by H2O2 exposure, as it reduced the generation of intracellular reactive oxygen species (ROS) in Chang cells. In addition, BMW up-regulated the production of antioxidant enzymes, such as catalase and superoxide dismutase (SOD), compared to H2O2-treated Chang cells lacking BMW. Moreover, BMW induced the expressions of nuclear Nrf2 and cytosolic HO-1 in H2O2-treated Chang cells. Interestingly, the treatment of ZnPP, HO-1 inhibitor, abolished the improvement in cell viability and intracellular ROS generation mediated by BMW treatment. In conclusion, this study suggests that BMW protects hepatocytes against H2O2-mediated cellular oxidative damage via up-regulation of the Nrf2/HO-1 signal pathway.


Assuntos
Extratos Celulares/farmacologia , Gastrópodes/química , Heme Oxigenase-1/metabolismo , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Transdução de Sinais , Taurina/farmacologia , Animais , Células Cultivadas , Humanos , Peróxido de Hidrogênio , Carne , Espécies Reativas de Oxigênio/metabolismo , Regulação para Cima
10.
Adv Exp Med Biol ; 1155: 611-626, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31468435

RESUMO

In this study, the antioxidant properties of Viviparus contectus (V. contectus) extract were evaluated for various radical scavenging activities, ferric reducing antioxidant power (FRAP), ABTS radical scavenging activity and oxygen radical absorbance capacity (ORAC). In addition, inhibition effect of the V. contectus extract against DNA scission induced by hydroxyl radical was measured. We also studied the protective effect of V. contectus extract against oxidative damage through measurements of intracellular reactive oxygen species (ROS) in Chang cells and zebrafish embryo. We found that V. contectus extract contains strong radical scavenging activities and antioxidant properties, which prevent tert-butylhydroperoxide (t-BHP)-induced oxidative stress, enhance cell viability, reduce ROS production, inhibit oxidative damage and improve mitochondrial function in Chang cells. Also, we determined that the V. contectus extract reduced ROS production mediated by t-BHP induced oxidative stress on zebrafish embryo.


Assuntos
Antioxidantes/farmacologia , Extratos Celulares/farmacologia , Gastrópodes/química , Estresse Oxidativo , Animais , Linhagem Celular , Embrião não Mamífero , Humanos , Espécies Reativas de Oxigênio/metabolismo , Peixe-Zebra , terc-Butil Hidroperóxido
11.
Adv Exp Med Biol ; 1155: 643-659, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31468437

RESUMO

Batillus cornutus (B. cornutus) is one of the gastropoda, which are distributed along the coast of China, Japan and South Korea and northeast area. In this study, we first identified the antioxidant effects of a B. cornutus meat (BM) enzymatic hydrolysate in H2O2-treated Vero cells. First of all, we prepared an Alcalase hydrolysate from BM (BMA) and revealed a high taurine content. Also, taurine rich BMA dose-dependently increased 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, reducing power and the higher oxygen radical absorbance capacity (ORAC) value. In addition, BMA significantly increased the cell viability via the down-regulation of intracellular reactive oxygen species (ROS) production, as well as the decreased formation of apoptotic bodies and sub-G1 DNA population in H2O2-treated Vero cells. Furthermore, BMA increased the expression of the anti-apoptotic molecule, Bcl-2, and decreased the expressions of Bax, p53 and cleaved PARP, all of which are pro-apoptotic molecules, in H2O2-treated Vero cells. Based on these results, this study suggests that BMA may be used as a potential protector on damage caused by oxidative stress.


Assuntos
Antioxidantes/farmacologia , Gastrópodes/química , Estresse Oxidativo , Hidrolisados de Proteína/farmacologia , Animais , Peróxido de Hidrogênio , Carne , Espécies Reativas de Oxigênio/metabolismo , Subtilisinas , Células Vero
12.
Adv Exp Med Biol ; 1155: 1001-1014, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31468463

RESUMO

Batillaria multiformis (B. multiformis) belong to gastropods. They live generally in the sandpit of the lagoons and the estuaries of the intertidal zone. Most of them are distributed in Korea, Japan and China. In this study, we investigated the anti-inflammatory potential of B. multiformis water extracts (BMW). The results showed that the extracts significantly decreased the production of nitric oxide (NO) and pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in LPS-induced RAW 264.7 macrophages. In addition, the extracts suppressed the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose dependent manner. Further investigation indicated that BMW suppressed phosphorylated c-Jun N-terminal kinase (JNK), extracellular regulated protein kinase (ERK) and p38 through the MAPK signaling pathway and influenced the NF-κB signaling pathway by suppressing the IκBα degradation in LPS-induced RAW 264.7 macrophages.


Assuntos
Anti-Inflamatórios/farmacologia , Extratos Celulares/farmacologia , Gastrópodes/química , Macrófagos/efeitos dos fármacos , NF-kappa B/metabolismo , Transdução de Sinais , Animais , Ciclo-Oxigenase 2/metabolismo , Citocinas/metabolismo , Lipopolissacarídeos , Camundongos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Células RAW 264.7 , Água
13.
Mar Pollut Bull ; 146: 54-59, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31426191

RESUMO

Toxic chemicals within and adsorbed to microplastics (0.05-5 mm) have the potential to biomagnify in food webs. However, microplastic concentrations in highly productive, coastal habitats are not well understood. Therefore, we quantified the presence of microplastics in a benthic community and surrounding environment of a remote marine reserve on the open coast of California, USA. Concentrations of microplastic particles in seawater were 36.59 plastics/L and in sediments were 0.227 ±â€¯0.135 plastics/g. Densities of microplastics on the surfaces of two morphologically distinct species of macroalgae were 2.34 ±â€¯2.19 plastics/g (Pelvetiopsis limitata) and 8.65 ±â€¯6.44 plastics/g (Endocladia muricata). Densities were highest in the herbivorous snail, Tegula funebralis, at 9.91 ±â€¯6.31 plastics/g, potentially due to bioaccumulation. This study highlights the need for further investigations of the prevalence and potential harm of microplastics in benthic communities at remote locations as well as human population centers.


Assuntos
Gastrópodes/química , Plásticos/análise , Água do Mar/análise , Alga Marinha/química , Caramujos/química , Animais , California , Ecossistema , Monitoramento Ambiental , Cadeia Alimentar , Gastrópodes/metabolismo , Sedimentos Geológicos/análise , Humanos , Plásticos/metabolismo , Alga Marinha/metabolismo , Caramujos/metabolismo , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismo
14.
Int J Biol Macromol ; 138: 49-56, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31301396

RESUMO

Polysaccharides with different structures have been reported to regulate iron uptake in opposite direction. The present study investigated the interaction between a sulfated polysaccharide (AGSP) from marine shellfishes and Fe(III)/Fe(II) in order to reveal the mechanism for the effect of AGSP on iron bioavailability. The Fe(III) chelating activity and resulting Fe(III)-chelates of AGSP and its desulfated derivative (dS-AGSP) were studied, their ferric reducing activity and Fe(II) chelating activity were compared, and their inhibition effects on the Fe absorption were evaluated by using Caco-2 cell models treated with Fe(III) and Fe(II). As indicated by the results, polysaccharides could chelate Fe(III) with the sulfate and carboxyl groups via FeO bond along with conformation change, resulting in unstable particles. Both AGSP and dS-AGSP inhibited the iron uptake in a dose-dependent manner. Notably, the chelating property of polysaccharides showed a negative correlation with the iron bioavailability while their reducing capability demonstrated a positive correlation. Moreover, sulfate substitution enhanced the chelating and reducing capabilities so to regulate the effects of AGSP on Fe absorption in contradictory ways. Thus, the present study elucidated the mechanism of the influence of AGSP on bioavailability of non-heme iron.


Assuntos
Gastrópodes/química , Quelantes de Ferro/química , Quelantes de Ferro/farmacologia , Ferro/metabolismo , Polissacarídeos/química , Polissacarídeos/farmacologia , Sulfatos/química , Animais , Células CACO-2 , Sequência de Carboidratos , Humanos , Quelantes de Ferro/metabolismo , Oxirredução/efeitos dos fármacos , Polissacarídeos/metabolismo
15.
Chemosphere ; 235: 876-884, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31284136

RESUMO

Among the radioactive pollutants 210Po is the most substantial one in terms of seafood safety due to its efficient accumulation in marine animals and high irradiation of its alpha emission. Mercury is a highly toxic metal for both marine organisms and human beings. Biomagnification of MeHg (methylmercury) through marine food chains has made Hg concern of ecotoxicology and seafood safety. In the current study, the bioaccumulation of 210Po and THg (total mercury) were determined in 20 mollusc species, including 8 bivalves, 7 gastropods and 5 cephalopods collected from the island of Gökçeada in the north-eastern Aegean Sea. The highest accumulation of 210Po and Hg was seen in bivalves and cephalopods, respectively. Elevated Hg concentrations in all body parts (arms, mantle and viscera) were observed in octopus' species. The results of this study suggests that filter feeder bivalves and gastropods have a capacity to concentrate 210Po in their bodies, whereas predator gastropods and cephalopods have a capacity to concentrate Hg in their bodies. 7.0 kg (3.2-14.2) bivalve flesh intake is adequate due to 210Po ingestion in the studied region to reach 1 mSv which is the annual committed effective dose. Octopus consumption of 705 g in a week alone is needed to reach Provisional Tolerable Weekly Intake (PTWI) of mercury, 5 µg kg-1 body weight. Due to very low non-fish seafood consumption in Turkey there is no risk of Hg intake and alpha radiation of 210Po above the limit values through mollusc consumption.


Assuntos
Bivalves/química , Cefalópodes/química , Contaminação de Alimentos/análise , Gastrópodes/química , Mercúrio/análise , Compostos de Metilmercúrio/análise , Polônio/análise , Poluentes Químicos da Água/análise , Animais , Organismos Aquáticos/química , Peixes , Cadeia Alimentar , Humanos , Medição de Risco , Alimentos Marinhos/análise , Turquia
16.
J Agric Food Chem ; 67(32): 8855-8867, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31343893

RESUMO

Abalone (Haliotis discus hannai) is a precious seafood in the market. It has been reported that biological active substances derived from abalone have anti-oxidative, anti-inflammatory, anti-bacterial, and anti-thrombosis potential. However, there were few studies to assess whether they have anti-cancer potential. In this study, we evaluated the anti-metastasis and anti-pro-angiogenic factors and mechanism of action of boiled abalone byproduct peptide (BABP, EMDEAQDPSEW) in human fibrosarcoma (HT1080) cells and human umbilical vein endothelial cells (HUVECs). The results demonstrated that BABP treatment significantly lowers migration and the invasion of HT1080 cells and HUVECs. BABP inhibits phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase (MMP) expression and activity by blocking mitogen-activated protein kinases (MAPKs) and NF-κB signaling and hypoxia-induced vascular endothelial growth factor (VEGF) secretion and hypoxia inducible factor (HIF)-1α accumulation through suppressing the AKT/mTOR signal pathway. BABP treatment inhibits VEGF-induced VEGFR-2 expression and tube formation in HUVECs. The effect of BABP on anti-metastatic and anti-vascular activity in HT1080 cells and HUVECs revealed that BABP may be a potential pharmacophore for tumor therapy in the future.


Assuntos
Inibidores da Angiogênese/farmacologia , Antineoplásicos/farmacologia , Gastrópodes/química , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Peptídeos/farmacologia , Resíduos/análise , Inibidores da Angiogênese/química , Inibidores da Angiogênese/isolamento & purificação , Animais , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/genética , Metaloproteinase 9 da Matriz/metabolismo , Metástase Neoplásica , Neovascularização Patológica/tratamento farmacológico , Neovascularização Patológica/genética , Neovascularização Patológica/metabolismo , Neovascularização Patológica/fisiopatologia , Peptídeos/química , Peptídeos/isolamento & purificação , Frutos do Mar/análise , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo
17.
Mar Drugs ; 17(7)2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31336763

RESUMO

Diverse non-methylene-interrupted (NMI) fatty acids (FAs) with odd-chain lengths have been recognized in triacylglycerols and polar lipids from the ovaries of the limpet Cellana toreuma, however their biological properties remain unclear. In this study, two previously unreported odd-chain NMI FAs, (12Z)-12,16-heptadecadienoic (1) and (14Z)-14,18-nonadecadienoic (2) acids, from the ovary lipids of C. toreuma were identified by a combination of equivalent chain length (ECL) values of their methyl esters and capillary gas chromatography-mass spectrometry (GC-MS) of their 3-pyridylcarbinol derivatives. On the basis of the experimental results, both 1 and 2 were synthesized to prove their structural assignments and to test their biological activity. The ECL values and electron impact-mass (EI-MS) spectra of naturally occurring 1 and 2 were in agreement with those of the synthesized 1 and 2. In an in vitro assay, both 1 and 2 activated protein phosphatase, Mg2+/Mn2+-dependent 1A (PPM1A) up to 100 µM in a dose-dependent manner.


Assuntos
Produtos Biológicos/farmacologia , Ativadores de Enzimas/farmacologia , Ácidos Graxos/farmacologia , Gastrópodes/química , Proteína Fosfatase 2C/metabolismo , Animais , Produtos Biológicos/síntese química , Ativadores de Enzimas/síntese química , Ensaios Enzimáticos , Ácidos Graxos/síntese química , Feminino , Células HL-60 , Humanos , Estrutura Molecular , Ovário/metabolismo , Proteínas Recombinantes/metabolismo
18.
Environ Pollut ; 250: 892-897, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31085475

RESUMO

To trace the circulation history of aquatic bioavailable Hg in the Antarctic, the species and isotopic compositions of Hg in sediment, Archaeogastropoda (Agas), Neogastropoda (Ngas), and fish collected from King George Island were studied in detail. Positive mass independent fractionation (MIF) was observed and positively correlated with the percentages of methylmercury (MeHg%) in Agas and Ngas, suggesting an effect of MeHg accumulation during trophic transfer on MIF signatures. However, both the ratios of Δ199Hg/δ202Hg and Δ199Hg/Δ201Hg indicated different circulation histories of Hg in Agas, Ngas, and fish. The microbial methylation in sediment was the primary source of MeHg in Agas and Ngas (Δ199Hg/δ202Hg ∼0, Δ199Hg/Δ201Hg ∼1.00). In contrast, the MeHg in fish (Δ199Hg/δ202Hg = 0.55 ±â€¯0.06, Δ199Hg/Δ201Hg = 1.19 ±â€¯0.17) came from the combined sources of residual MeHg which had sunk from the surface water and microbial-methylated MeHg in sediments, and the bioavailable Hg in the sediments contributed to approximately 44% of the total Hg in fish. Subsequently, the Δ199Hg values of bioavailable MeHg and IHg in sediments were quantitatively calculated, which provided key end-member information for future source apportionment in the Antarctic and other pelagic regions. It was also found that the Hg accumulated in Agas and Ngas had no history of MeHg photo-degradation, indicating that the methylated Hg in benthic zones suffered little photo-degradation and thus presented high bioavailability and environmental risk.


Assuntos
Organismos Aquáticos/química , Monitoramento Ambiental/métodos , Mercúrio/análise , Compostos de Metilmercúrio/análise , Poluentes Químicos da Água/análise , Animais , Regiões Antárticas , Organismos Aquáticos/metabolismo , Fracionamento Químico , Peixes/metabolismo , Cadeia Alimentar , Gastrópodes/química , Gastrópodes/metabolismo , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiologia , Ilhas , Isótopos de Mercúrio/análise , Microbiota , Água do Mar/química , Água do Mar/microbiologia
19.
J Sci Food Agric ; 99(12): 5550-5557, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31115054

RESUMO

BACKGROUND: Owing to the comprehensive application of quinoxaline-1,4-dioxides (QdNOs) in aquaculture, QdNOs and metabolites are often detected in marine food, including abalone. QdNOs are reported to exhibit cytotoxicity, photoallergy, mutagenicity, and carcinogenicity activities. To monitor for contamination of QdNOS in abalone and assess dietary exposure, a simple and reliable analytical method for the detection of QdNOs and their major metabolites was developed. RESULTS: This work is the first to present a simple and fast pretreatment procedure coupled with high-performance liquid chromatography tandem positive-mode electrospray ionization mass spectrometry (LC-MS/MS) for tracing of major metabolites of QdNOs in abalone. Extraction steps were simplified by the use of methanol and ethyl acetate containing 0.1% formic acid instead of more complicated acidolysis and enzymolysis pretreatment procedures. High-sensitive characters were obtained with limits of detection ranged from 0.16 to 2.1 µg kg-1 for QdNOs and their major metabolites. CONCLUSION: These results indicate that the LC-MS/MS method developed could be applied for QdNOs and major metabolites detection in actual samples. Considering the large production and consumption of abalone in Shandong Province, China, this work will also contribute to the further understanding of the often-ignored exposure pathway of QdNOs. © 2019 Society of Chemical Industry.


Assuntos
Antibacterianos/química , Antibacterianos/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Gastrópodes/química , Quinoxalinas/análise , Quinoxalinas/metabolismo , Alimentos Marinhos/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Animais , China , Contaminação de Alimentos/análise , Gastrópodes/metabolismo , Espectrometria de Massas em Tandem/métodos
20.
Artigo em Inglês | MEDLINE | ID: mdl-31100041

RESUMO

A rapid screening method is described for the determination of paralytic shellfish toxins (PST), in fresh marine vectors (bivalves and gastropods), at levels ranging from 0.05 to 5.0 mg STX-eq kg-1. PST are extracted from marine vector homogenates with acetic acid according to the Pre-COX-LC-FLD method. At the same time, the obtained extract is oxidised simultaneously in hydrogen peroxide and periodate oxidate to determine PST, non-N-hydroxylated and N-hydroxylated toxins, respectively. Then, they are analysed using a microplate fluorometer (Ex: 335 nm/Em: 405 nm). All the samples were compared with the liquid chromatography post-column oxidation method. Recoveries of PST added to fresh and processed marine vectors averaged 93.9% with a coefficient of variation of 6.1%. Both methods showed a good linear regression (r2 = 0.97). The method shows good intra- and inter-day precisions with a relative coefficient of variation of ≈ 3.8% and 5.7%, respectively. The limit of quantification of the rapid screening fluorescence method was ≈ 0.082 mg STX-eq kg-1, with ≤5% false positives. The established rapid screening fluorescence methods offer highly effective and verifiable pre-analyses of PST contamination in marine vectors and can be used for routine screening of the PST in seafood before formal identification by confirmatory methods (Pre-COX LC-FLD method, Lawrence method).


Assuntos
Bivalves/química , Fluorescência , Contaminação de Alimentos/análise , Gastrópodes/química , Toxinas Marinhas/análise , Intoxicação por Frutos do Mar , Animais , Cromatografia Líquida , Avaliação Pré-Clínica de Medicamentos , Conformação Molecular , Espectrometria de Fluorescência
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