Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 265
Filtrar
1.
Nat Commun ; 12(1): 5127, 2021 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-34493721

RESUMO

Intricate color patterns are a defining aspect of morphological diversity in the Felidae. We applied morphological and single-cell gene expression analysis to fetal skin of domestic cats to identify when, where, and how, during fetal development, felid color patterns are established. Early in development, we identify stripe-like alterations in epidermal thickness preceded by a gene expression pre-pattern. The secreted Wnt inhibitor encoded by Dickkopf 4 plays a central role in this process, and is mutated in cats with the Ticked pattern type. Our results bring molecular understanding to how the leopard got its spots, suggest that similar mechanisms underlie periodic color pattern and periodic hair follicle spacing, and identify targets for diverse pattern variation in other mammals.


Assuntos
Gatos/genética , Regulação da Expressão Gênica no Desenvolvimento , Pigmentação/genética , Animais , Animais Domésticos , Gatos/crescimento & desenvolvimento , Epiderme/crescimento & desenvolvimento , Epiderme/metabolismo , Genótipo , Peptídeos e Proteínas de Sinalização Intercelular/genética , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Queratinócitos/metabolismo , Mutação , Fenótipo , Análise de Célula Única , Pele/anatomia & histologia , Pele/crescimento & desenvolvimento , Pele/metabolismo , Via de Sinalização Wnt
3.
J Feline Med Surg ; 22(6): 476-483, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-31184248

RESUMO

OBJECTIVES: The aims of the present study were to monitor, by radiographic examination, the skeletal development of the pelvis and the femorotibial joints of the domestic cat from the first week of life until the closing of the growth plates. METHODS: Radiographic examinations were collected from 15 domestic cats at weekly intervals during the first month and every 2 weeks from the second to the fourth month of age. After that, examinations were performed monthly until the age of 18 months. RESULTS: The ischiopubic growth plate closed at 2 months of age, followed by the fusion of the iliopubic, ilioischial, proximal femoral, greater trochanter and proximal fibular growth plates. The distal femur and proximal tibial growth plates were the last to close, with fusion occurring at 18 months. The mean time to closure of the iliopubic, ilioischial and distal femoral growth plates was shorter in females. The ossification centers first appeared, in ascending order, beginning with the lesser trochanter, followed by the greater trochanter, proximal fibular epiphysis, tibial tuberosity, patella, ischial tuberosity and lateral sesamoid of the popliteus muscle. CONCLUSIONS AND RELEVANCE: The complete closure of the growth plates of domestic cats occurs at approximately 18 months of age. Skeletal maturation at approximately 18 months of age is an important parameter to be considered in radiographic evaluation of certain skeletal changes, evolution of fractures and nutritional imbalance.


Assuntos
Gatos/crescimento & desenvolvimento , Fíbula/crescimento & desenvolvimento , Articulação do Quadril/crescimento & desenvolvimento , Articulações/crescimento & desenvolvimento , Pelve/crescimento & desenvolvimento , Radiografia/veterinária , Tíbia/crescimento & desenvolvimento , Animais , Feminino , Fíbula/diagnóstico por imagem , Articulação do Quadril/diagnóstico por imagem , Articulações/diagnóstico por imagem , Masculino , Pelve/diagnóstico por imagem , Tíbia/diagnóstico por imagem
4.
J Feline Med Surg ; 22(4): 322-328, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-30982390

RESUMO

OBJECTIVES: The objective of this study was to assess the utility of using body weight for age determination in kittens. METHODS: Medical records were reviewed for serial body weight measurements collected from neonatal kittens (up to 8 weeks of age) from a breeding colony of specific pathogen-free domestic shorthair cats and for single-point body weight measurements of privately owned pediatric kittens (6-20 weeks of age) presenting for elective sterilization. Body weights were compared with known dates of birth and age assessed by dental eruption in combination with developmental characteristics. RESULTS: The coefficient of determination (R2) between age and body weight in longitudinally sampled neonatal kittens was 0.88, while that for pediatric kittens sampled at a single time point was 0.54. Among neonatal kittens, predicted age based on the 1 lb (0.45 kg) of body weight gain per month of age guideline corresponded to within 1 week of actual age for 243 (98.8%), 234 (95.1%), 203 (82.5%) and 191 (77.6%) kittens at 2, 4, 6 and 8 weeks of age, respectively. Among pediatric kittens, predicted age based on this guideline corresponded to within 1 week of actual age for 24 (77.4%), 411 (67.5%), 170 (57.0%), 96 (46.6%), 23 (28.8%), 15 (27.8%), one (25%) and five (17.9%) kittens at 6, 8, 10, 12, 14, 16, 18 and 20 weeks, respectively. CONCLUSIONS AND RELEVANCE: Body weight was an effective means of predicting age in kittens through 10 weeks of age. Factors other than body weight should be considered when estimating kitten age beyond that time point.


Assuntos
Envelhecimento/fisiologia , Animais Recém-Nascidos/crescimento & desenvolvimento , Peso Corporal/fisiologia , Gatos/crescimento & desenvolvimento , Animais
5.
Theriogenology ; 130: 49-61, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30865874

RESUMO

The study describes the sequence of ultrastructural changes of the endometrial lining epithelium, first in the primordial paramesonephric ducts, then in the differentiating uterine horns, and finally in the mature uterus. The research material comprised female fetuses of the domestic cat, aged 33-63 days post conceptionem (p.c.), and mature females. The ultrastructural observations of the developing epithelium were conducted using transmission electron microscopy (TEM) and light microscopy (LM). The results revealed two basic stages in the development of the uterine mucosal epithelium in the domestic cat: the development of the epithelium of the uterine segment of the paramesonephric ducts, lasting from day 33 to day 42 p.c., and the differentiation of this epithelium into the mucosal epithelium of the uterus, starting after day 42 p.c. and lasting until the end of the prenatal period. The epithelium of the uterine segments of the paramesonephric ducts is pseudostratified with a flat surface, all cells being characterized by the same distribution of desmosomes, ribosomes, rough endoplasmic reticulum (rER) cisternae, and mitochondria in the cytoplasm. The differentiating epithelium of the uterus after day 51 p.c. is pseudostratified with a varied height. In the epithelial cells there are increases of (i) the number and complexity of the junctional complexes, (ii) the number of invaginations of the lateral cell membranes, and (iii) the number of elongated mitochondria, and also there appear distended cisternae of the rER, lipid droplets and clusters of glycogen, which suggest increasing metabolism and secretory activity of the cells during the transformation of the epithelium.


Assuntos
Gatos/crescimento & desenvolvimento , Epitélio/embriologia , Epitélio/ultraestrutura , Desenvolvimento Fetal , Útero/embriologia , Útero/ultraestrutura , Animais , Feminino
6.
Theriogenology ; 128: 62-73, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30743105

RESUMO

Toll-like receptors (TLRs) are important molecules, which provide protection against infections of the reproductive tract. This study demonstrates for the first time the expression and localization patterns of TLRs in the caput, corpus and cauda segments of the epididymal duct (ED) and the vas deferens (VD) of adult domestic cats using immunohistochemistry and western blotting. While immunoblot analyses revealed relatively similar protein levels for TLRs 2, 4, 5, and 9 in three segments of the ED, the protein levels of TLR2 and TLR4 in the VD were found to be significantly higher than those measured in the ED segments (P < 0.05). On the other hand, immunostaining showed that TLRs exhibited regional- and cell-specific localization patterns. TLR2 and TLR5 were immunolocalized to the nucleus and cytoplasm of the principal cells in all ducts. TLR4 was restricted to the stereocilia, and TLR9 was located in the cytoplasm of the principal cells. Narrow cells displayed positive immunoreactions for TLR4 and TLR5. The basal cells of the different ED segments were positive for all four TLRs. TLR2, TLR5 and TLR9 were detected in the cytoplasmic droplets of the spermatozoa. TLR4 and TLR9 were detected along the entire length of the sperm tail, whilst TLR2 and TLR5 were absent in the midpiece. TLR2 and TLR5 were also detected in the equatorial segment of the sperm head. These results suggest that TLR2, TLR4, TLR5 and TLR9 are important not only for the protection of the ED, VD and spermatozoa but also for the maturation and storage of spermatozoa in the ED and VD, respectively.


Assuntos
Gatos/metabolismo , Epididimo/metabolismo , Receptores Toll-Like/metabolismo , Ducto Deferente/metabolismo , Animais , Western Blotting/veterinária , Gatos/crescimento & desenvolvimento , Imuno-Histoquímica/veterinária , Masculino , Receptor 2 Toll-Like/análise , Receptor 2 Toll-Like/metabolismo , Receptor 4 Toll-Like/análise , Receptor 4 Toll-Like/metabolismo , Receptor 5 Toll-Like/análise , Receptor 5 Toll-Like/metabolismo , Receptor Toll-Like 9/análise , Receptor Toll-Like 9/metabolismo , Receptores Toll-Like/análise
7.
J Morphol ; 279(12): 1764-1775, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30443927

RESUMO

The study describes the morphology and topography of internal reproductive organs in the domestic cat from the early prenatal period to maturity, using macroscopic and scanning electron microscope (SEM) observations with three-dimensional (3D) reconstructions. Fifty-seven female cat fetuses aged between the 27th and 63rd day postconception (p.c.), two newborn cats, three juveniles (3-month-old) cats, and three mature (12-month-old) cats were used in the study. The age of fetuses was determined on the basis of the growth curve for the domestic cat. The rudiments of cat ovaries develop on the ventral surface of the mesonephroi and within 30 days p.c. move to the sides of the abdominal cavity, which is similar to the position of the ovaries in the adult cat. The mesonephroi regress at about the 50th day p.c., when the residual mesonephric ducts are still found in the lower part of the body of the uterus. The paramesonephric ducts develop on the lateral surface of the mesonephroi and by the 45th day p.c., differentiate into the uterine tubes and the uterus. The arrangement of the paramesonephric ducts in the abdominal cavity changes from the U- to the V-shaped system. The final topography of the uterine tubes is established between the 54th and 60th day p.c., as the uterine tubes become convoluted. Before the 54th day p.c., the uterine horns undergo rapid elongation and convolution, forming the W-shaped system. By the third month of postnatal life, the uterine horns become straight, as in the adult cat.


Assuntos
Gatos/anatomia & histologia , Gatos/crescimento & desenvolvimento , Genitália/anatomia & histologia , Genitália/ultraestrutura , Imageamento Tridimensional , Microscopia Eletrônica de Varredura , Animais , Animais Recém-Nascidos , Tubas Uterinas , Feminino , Genitália/crescimento & desenvolvimento , Ovário , Útero
8.
Reprod Domest Anim ; 53 Suppl 3: 74-78, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30474334

RESUMO

The aim of this study was to evaluate two-dimensional and Doppler ultrasonographic changes of feline ovaries before and during puberty. Nine, 3-month-old female cats were followed until puberty (Day 1). Two-dimensional and Doppler ultrasound evaluations of the ovaries were carried out on Days -50, -20, -7 and 1. Longitudinal and transverse sections of the ovaries were measured and all anechoic spherical structures were considered to be follicles. The number of follicles >1 mm and the maximum diameter of the largest follicle were recorded. Peak systolic velocity and end diastolic velocity of intraovarian arteries were also measured to automatically calculate the resistive index (RI). The mean ovarian longitudinal diameter increased gradually throughout the study from 8.6 to 10.7 mm (p < 0.05). While four cats presented multiple anechoic spherical structures <1 mm diameter throughout the study, the remaining five animals had these structures only on Days -50 and -20. On Days -20, -7 and 1, the mean number of follicles were 1.4 ± 0.7, 2.5 ± 0.8 and 4.8 ± 1 respectively (p < 0.01). The largest follicles at the same time points were 1.1 ± 0.2 mm, 1.9 ± 0.3 mm and 2.6 ± 0.5 mm respectively (p < 0.05). The RI of the intraovarian arteries declined throughout the study period (p < 0.01). It is concluded that, in female cats, ovarian dimensions, follicle number and intraovarian blood flow increased from 3 months of age to puberty.


Assuntos
Gatos/crescimento & desenvolvimento , Ovário/crescimento & desenvolvimento , Maturidade Sexual/fisiologia , Ultrassonografia/veterinária , Animais , Feminino , Hemodinâmica , Folículo Ovariano/crescimento & desenvolvimento , Ovário/anatomia & histologia , Ovário/irrigação sanguínea , Ovário/diagnóstico por imagem , Ultrassonografia Doppler/veterinária
9.
J Anim Physiol Anim Nutr (Berl) ; 102(5): 1401-1410, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29920783

RESUMO

Overweight and obesity are multifactorial diseases caused by an imbalance in energy metabolism. An underlying genetic predisposition is often a factor in these conditions. In the cat breeding family of the Institute of Animal Nutrition at the Vetsuisse Faculty, University of Zurich, a segregating overweight phenotype with a genetic contribution was observed. From this breeding family, 26 kittens were followed from birth up to 8 months of age. During this time, food intake was measured using an automatic feeding station, and energy expenditure was investigated using indirect calorimetry at the ages of 4 and 6 months. Dual-energy X-ray absorptiometry (DEXA) was performed and blood glucose, leptin and insulin were measured at the ages of 4, 6 and 8 months. The kittens were also weighed daily for the first 2 weeks of life, every second day until weaning and once per week until 8 months of age. The body condition score (BCS) was evaluated monthly between 2 and 8 months of age. The main finding of this study is that a predisposition to overweight is connected to a higher food intake early in life, with no significant alterations in energy expenditure. The leptin blood levels were related to body fat percentage, and insulin sensitivity did not seem to be affected.


Assuntos
Doenças do Gato/epidemiologia , Gatos , Ingestão de Energia/fisiologia , Metabolismo Energético/fisiologia , Obesidade/veterinária , Animais , Composição Corporal , Calorimetria Indireta , Doenças do Gato/prevenção & controle , Gatos/crescimento & desenvolvimento , Gatos/metabolismo , Ingestão de Alimentos , Feminino , Leptina/sangue , Obesidade/epidemiologia , Obesidade/prevenção & controle
10.
Arq. bras. med. vet. zootec. (Online) ; 70(6): 1775-1783, nov.-dez. 2018. tab, ilus
Artigo em Português | ID: biblio-970421

RESUMO

O objetivo deste estudo foi caracterizar as doenças dos gatos domésticos provenientes dos casos de necropsia e histopatologia do Hospital Veterinário da Universidade Federal de Minas Gerais (HV/UFMG), de 2005 a 2014. Foram analisados 408 exames de necropsia e 197 de biópsias, segundo o sexo, a faixa etária e a raça. Os diagnósticos mais frequentes incluíram doenças infecciosas/inflamatórias ou parasitárias (22,5%), agentes físicos (18,1%), doenças proliferativas (15,2%) e degenerativas (13,5%). Politraumatismo (10,8%) foi responsável pelo maior número de mortes em felinos, sendo as chances três vezes maiores em animais com até 24 meses de idade (P=0,005 OR 3,47 [IC 95%: 1,40-8,57]). Neoplasias epiteliais corresponderam a 26 diagnósticos, sendo 20 (4,9%) casos de malignidade. A ocorrência de carcinoma e seus subtipos foi 18 vezes maior em gatos idosos (P<0,01 OR 18,15 [IC 95%: 7,41-44,45]). A insuficiência renal crônica foi mais frequente em gatos com mais de 120 meses (P=0,01). Machos apresentaram nove vezes mais chances de desenvolver doenças do trato urinário inferior quando comparados às fêmeas (P=0,001 OR 9,50 [IC 95%: 2,78-32,48]). Em relação às biópsias, animais adultos a idosos foram 10 vezes mais representados (P<0,001 OR 10,8 [IC 95%: 3,22-36,79]).(AU)


The aim of this study was to characterize the diseases of domestic cats based on necropsy and histopathological examinations at the Veterinary Hospital of the Universidade Federal de Minas Gerais (HV/UFMG) from 2005 to 2014. A total of 408 necropsy and 197 biopsy samples were analyzed according to gender, age, and breed. The most frequent diagnoses included infectious/inflammatory or parasitic diseases (22.5%), physical agents (18.1%), proliferative (15.2%), and degenerative (13.5%) diseases. Polytrauma (10.8%) was responsible for the highest number of feline deaths, with the odds three times higher in animals up to 24 months of age (P= 0.005 OR 3.47 [95% CI: 1.40-8.57]). Epithelial neoplasms corresponded to 26 diagnoses, with 20 (4.9%) malignant cases. The occurrence of carcinoma and its subtypes was 18-fold higher in older cats (P< 0.01 OR 18.15 [95% CI: 7.41-44.45]). Chronic renal failure was more frequent in cats over 120 months (P= 0.01). Males were nine times more likely to develop lower urinary tract diseases when compared to females (P= 0.001 OR 9.50 [95% CI: 2.78-32.48]). Regarding the biopsies, adult to elderly animals were ten times more represented (P< 0.001 OR 10.8 [95% CI: 3.22-36.79]).(AU)


Assuntos
Animais , Gatos , Autopsia/veterinária , Gatos/classificação , Gatos/crescimento & desenvolvimento , Gatos/anormalidades , Inquéritos Epidemiológicos
11.
Theriogenology ; 99: 119-123, 2017 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-28708492

RESUMO

In this study it was determined the progression of uterine gland development from late gestation to puberty in domestic felids. Cell proliferation patterns for luminal (LE), glandular epithelium (GE) as well as stroma (S) were also described. Twenty-four uteri from female kittens: 45 and 65 days of gestation and 1 to 5, 8, 12, 16, 20 and 24 weeks postnatally were obtained. Uterine cross-sections were submitted for routine histological and immunohistochemical quantification of proliferating cell nuclear antigen (PCNA) techniques. Although prenatal uteri presented no indication of adenogenesis, 1 week old uteri revealed an incipient budding of the LE. During the second week budding increased and a mild degree of tubulogenesis of the GE into the stroma was detected. From the third to fifth weeks coiling, branching and cross-sections of glands appeared. These latter findings were more evident in week 8 when GE began to penetrate through much of the S to week 24. PCNA immunostaining revealed that DNA synthesis decreased throughout the study in the 3 cell compartments; (P < 0.01). Luminal proliferation began prenatally, it maintained up to postnatal week 8 to markedly decrease to puberty (P < 0.01). From postnatal week 3 up to week 8, GE mitotic activity was elevated becoming low thereafter (P < 0.01). Stroma actively proliferated prenatally (P < 0.01), diminishing up to week 8 (P < 0.01) and again during the last weeks (P < 0.01) of the study. It was concluded that, in domestic felids, proliferation of LE begins prenatally, histological uterine adenogenesis commenced during the first postnatal week and both events concluded by postnatal weeks 5-8.


Assuntos
Gatos/crescimento & desenvolvimento , Endométrio/anatomia & histologia , Endométrio/crescimento & desenvolvimento , Ciclo Menstrual/fisiologia , Maturidade Sexual/fisiologia , Animais , Feminino , Progestinas/fisiologia
12.
Anat Histol Embryol ; 46(4): 397-404, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28677231

RESUMO

The neocortex plays a key role in cognition, volitional motor control and sensory perception and has undergone tremendous expansion during evolution. The mature neocortex consists of radially aligned neurons that are arranged in six layers. Layers II-VI are often split into two groups: deep and upper layers, both building up the so-called cortical plate during embryonic and foetal development. So far cortical neurogenesis, including the generation of deep and upper layers, has mostly been studied in laboratory rodents and primates. However, precise data for most companion animals are lacking. This study determined the main period of neurogenesis, specifically the timing of deep and upper layer generation, in the developing domestic cat, pig and sheep neocortex using immunohistochemistry for specific neuronal markers, that is Tbr1 and Brn2. We found that the general sequence of neural events is preserved among cat, pig, sheep and other mammalian species. However, we observed differences in the timing of the overall cortical neurogenic period and occurrence of distinct neural events when these three species were compared. Moreover, our data provide further evidence that the cortical neurogenic period and gestation length might be tightly related. Together, these data expand our current understanding of neocortex development and are important for future studies investigating neocortex development and expansion especially in companion animals.


Assuntos
Gatos/embriologia , Neocórtex/embriologia , Ovinos/embriologia , Suínos/embriologia , Animais , Gatos/crescimento & desenvolvimento , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Imuno-Histoquímica/veterinária , Modelos Lineares , Microscopia Confocal/veterinária , Neocórtex/química , Neocórtex/crescimento & desenvolvimento , Neocórtex/metabolismo , Ovinos/crescimento & desenvolvimento , Estatística como Assunto , Suínos/crescimento & desenvolvimento , Proteínas com Domínio T/genética , Proteínas com Domínio T/metabolismo , Telencéfalo/química
13.
J Feline Med Surg ; 19(12): 1224-1230, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28195504

RESUMO

Objectives The purpose of this study was to assess efficacy of deslorelin, a gonadotropin-releasing hormone (GnRH) agonist marketed in Europe for the control of male dog reproduction, for the postponement of puberty in queens. Methods Nine prepubertal queens aged 3-9 months were selected for this study; their general and reproductive health was checked through clinical, haematological, vaginal cytology and hormonal tests. Following treatment with a 4.7 mg deslorelin implant, each cat received a monthly clinical examination and blood was collected for hormonal assay every third month. Cats were monitored for 14.1 ± 5.2 (range 7-23) months. Results All cats were in good body condition and normal health prior to treatment. Their health status remained unchanged throughout the study and no significant variation was observed with regard to serum progesterone or oestradiol. Seven days post-treatment, 1/9 queens showed signs of heat, and one other queen showed complete vaginal keratinisation. No other signs of heat were subsequently observed in any other queen. Five queens were lost during the study after 7, 7, 16, 17 and 18 months of observation (during which time they did not show signs of heat). By the end of the study, no sign of puberty was observed in the four remaining queens at 21-36 months of age. Conclusions and relevance A 4.7 mg deslorelin implant was able to suppress the feline pituitary-gonadal axis, leading to postponement of puberty for up to 21-36 months in the four queens that completed the study. Deslorelin can be considered as a safe method to postpone puberty in queens.


Assuntos
Gatos/crescimento & desenvolvimento , Anticoncepção/veterinária , Inibidores Enzimáticos/farmacologia , Hormônio Liberador de Gonadotropina/agonistas , Maturidade Sexual/efeitos dos fármacos , Pamoato de Triptorrelina/análogos & derivados , Animais , Gatos/sangue , Implantes de Medicamento , Feminino , Pamoato de Triptorrelina/farmacologia
14.
J Feline Med Surg ; 19(4): 424-434, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26927818

RESUMO

Objectives The objective of this study was to determine if two raw feline diets were nutritionally adequate for kittens. Methods Twenty-four 9-week-old kittens underwent an Association of American Feed Control Officials' (AAFCO) 10 week growth feeding trial with two raw diet groups and one cooked diet group (eight kittens in each). Morphometric measurements (weight, height and length), complete blood counts, serum chemistry, whole blood taurine and fecal cultures were evaluated. Results Overall, the growth parameters were similar for all diet groups, indicating the two raw diets used in this study supported feline growth, within the limitations of an AAFCO growth feeding trial. Kittens fed the raw diets had lower albumin ( P = 0.010) and higher globulin ( P = 0.04) levels than the kittens fed the cooked diet. These lower albumin levels were not clinically significant, as all groups were still within normal age reference intervals. A red cell microcytosis ( P = 0.001) was noted in the combination raw diet group. Increases in fecal Clostridium perfringens were noted in all groups, along with positive fecal Salmonella serovar Heidelberg and Clostridium difficile toxin in the combination raw diet group. Conclusions and relevance The majority of the parameters for feline growth were similar among all groups, indicating the two raw diets studied passed an AAFCO growth trial. In theory, it is possible to pass an AAFCO growth trial but still have nutrient deficiencies in the long term due to liver and fat storage depots. Some of the raw feeders had elevated globulin and microcytosis, likely associated with known enteropathogenic exposure. Disease risks to both pets and owners are obvious. Additional research in this area is needed to investigate the impact of raw diets on the health of domestic cats.


Assuntos
Ração Animal/análise , Gatos/fisiologia , Culinária , Dieta/veterinária , Fenômenos Fisiológicos da Nutrição Animal , Animais , Animais Recém-Nascidos/crescimento & desenvolvimento , Animais Recém-Nascidos/fisiologia , Gatos/crescimento & desenvolvimento , Fezes/microbiologia , Feminino , Masculino , Resultado do Tratamento
15.
PLoS One ; 11(12): e0168144, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27942045

RESUMO

Neutering is a significant risk factor for obesity in cats. The mechanisms that promote neuter-associated weight gain are not well understood but following neutering, acute changes in energy expenditure and energy consumption have been observed. Metabolic profiling (GC-MS and UHPLC-MS-MS) was used in a longitudinal study to identify changes associated with age, sexual development and neutering in male cats fed a nutritionally-complete dry diet to maintain an ideal body condition score. At eight time points, between 19 and 52 weeks of age, fasted blood samples were taken from kittens neutered at either 19 weeks of age (Early Neuter (EN), n = 8) or at 31 weeks of age (Conventional Neuter (CN), n = 7). Univariate and multivariate analyses were used to compare plasma metabolites (n = 370) from EN and CN cats. Age was the primary driver of variance in the plasma metabolome, including a developmental change independent of neuter group between 19 and 21 weeks in lysolipids and fatty acid amides. Changes associated with sexual development and its subsequent loss were also observed, with differences at some time points observed between EN and CN cats for 45 metabolites (FDR p<0.05). Pathway Enrichment Analysis also identified significant effects in 20 pathways, dominated by amino acid, sterol and fatty acid metabolism. Most changes were interpretable within the context of male sexual development, and changed following neutering in the CN group. Felinine metabolism in CN cats was the most significantly altered pathway, increasing during sexual development and decreasing acutely following neutering. Felinine is a testosterone-regulated, felid-specific glutathione derivative secreted in urine. Alterations in tryptophan, histidine and tocopherol metabolism observed in peripubertal cats may be to support physiological functions of glutathione following diversion of S-amino acids for urinary felinine secretion.


Assuntos
Envelhecimento , Gatos/metabolismo , Metaboloma , Orquiectomia/veterinária , Desenvolvimento Sexual , Animais , Gatos/sangue , Gatos/crescimento & desenvolvimento , Ingestão de Alimentos , Metabolismo Energético , Masculino , Hormônios Peptídicos/metabolismo , Ganho de Peso
16.
Int J Dev Neurosci ; 54: 32-38, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27568056

RESUMO

Three-dimensional reconstruction of developing fiber pathways is essential to assessing the developmental course of fiber pathways in the whole brain. We applied diffusion spectrum imaging (DSI) tractography to five juvenile ex vivo cat brains at postnatal day (P) 35, when the degree of myelination varies across brain regions. We quantified diffusion properties (fractional anisotropy [FA] and apparent diffusion coefficient [ADC]) and other measurements (number, volume, and voxel count) on reconstructed pathways for projection (cortico-spinal and thalamo-cortical), corpus callosal, limbic (cingulum and fornix), and association (cortico-cortical) pathways, and characterized regional differences in maturation patterns by assessing diffusion properties. FA values were significantly higher in cortico-cortical pathways within the right hemisphere compared to those within the left hemisphere, while the other measurements for the cortico-cortical pathways within the hemisphere did not show asymmetry. ADC values were not asymmetric in both types of pathways. Interestingly, tract count and volume were significantly larger in the left thalamo-cortical pathways compared to the right thalamo-cortical pathways. The bilateral thalamo-cortical pathways showed high FA values compared to the other fiber pathways. On the other hand, ADC values did not show any differences across pathways studied. These results demonstrate that DSI tractography successfully depicted regional variations of white matter tracts during development when myelination is incomplete. Low FA and high ADC values in the cingulum bundle suggest that the cingulum bundle is less mature than the others at this developmental stage.


Assuntos
Encéfalo/anatomia & histologia , Gatos/anatomia & histologia , Gatos/crescimento & desenvolvimento , Imagem de Tensor de Difusão , Substância Branca/diagnóstico por imagem , Substância Branca/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos , Anisotropia , Processamento de Imagem Assistida por Computador , Vias Neurais/diagnóstico por imagem , Vias Neurais/crescimento & desenvolvimento
17.
Ultrastruct Pathol ; 39(4): 245-54, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25867690

RESUMO

The ultrastructure of the developing bronchiolar cell was studied in six age groups: prenatal (60 d post-conception); postnatal (1-, 7-, 14- and 21-day-old); and adult. Following intratracheal fixation, the lung tissue was processed for scanning and transmission electron microscopy. The lining of terminal bronchioles consists of cuboidal to columnar nonciliated bronchiolar cells (NBCs) and ciliated with or without microvilli. NBCs were recognized by indented centrally located nucleus. The apical surface extended beyond the surface of neighboring cells and was covered by minute microvilli, except in prenatal kittens. The NBCs of the adult were characterized by abundant mitochondria and glycogen inclusions. In prenatal kittens, the cytoplasm was filled with patches of alpha and beta form of glycogen. Postnatally, glycogen was reduced in quantity, became scattered throughout the cytoplasm and was predominantly of the beta form. Islands of cytoplasm, separated from the apical cytoplasm were observed in the lumen of adult bronchioles. This suggests an apocrine mode of secretion. The NBCs attain maturity by three weeks of age.


Assuntos
Bronquíolos/crescimento & desenvolvimento , Bronquíolos/ultraestrutura , Gatos/crescimento & desenvolvimento , Células Epiteliais/ultraestrutura , Mucosa Respiratória/crescimento & desenvolvimento , Mucosa Respiratória/ultraestrutura , Animais , Gatos/anatomia & histologia , Feminino , Masculino , Microscopia Eletrônica
19.
PLoS One ; 9(2): e89557, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24586869

RESUMO

The initial cause of post-neutering weight gain in male cats is not entirely known. There is evidence that energy intake (EI) increases rapidly post-neutering, but it is not clear if neutering also decreases energy expenditure (EE) prior to weight gain. Thus, the purpose of this study was to determine if a decrease in EE contributes to the initial shift toward positive energy balance in neutered male cats. To determine the influence of neutering on EE independent of changes in EI and body weight (BW), male cats were fed at their pre-neutering maintenance EI and EE was measured at 4 days pre-neutering, 3-4 days post-neutering, and 9 days post- neutering. Ad libitum food access was then provided for 6 months. Body composition was measured and blood samples collected for serum chemistry at pre-neutering and 7 days, 13 days and 6 months post-neutering. Total energy expenditure (TEE) adjusted for lean body mass (LBM) did not change in cats from pre-neutering to 9 days post-neutering. However, TEE adjusted for BW and resting energy expenditure adjusted for either LBM or BW showed a small, but significant (P<0.05) increase from pre-neutering to 9 days post-neutering. When allowed free choice food access, cats showed significant increases of food intake (FI) and BW. Circulating concentrations of ghrelin increased, while adiponectin levels decreased following neutering. The results of this study indicate that initial post-neutering weight gain in male cats results from increased FI and not decreased EE. Long-term control of FI should be initiated after neutering to prevent hyperphagia and weight gain in male cats.


Assuntos
Composição Corporal/fisiologia , Peso Corporal/fisiologia , Gatos/crescimento & desenvolvimento , Ingestão de Alimentos/fisiologia , Metabolismo Energético/fisiologia , Orquiectomia/veterinária , Animais , Masculino
20.
Vet Clin North Am Small Anim Pract ; 44(2): 205-19, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24580987

RESUMO

This article provides clinically relevant and applicable information about normal biochemical values in puppies and kittens younger than 6 months, and is intended to provide practical guidelines for the interpretation of serum biochemical results in these young animals. At present there are no published sets of normal hematologic reference ranges for mixed-breed puppies and kittens younger than 6 months. Reference-value sets for closed research colonies composed of a few selected breeds are available, which help to provide insight into trends in normal hematologic and biochemical values for puppies and kittens.


Assuntos
Gatos/sangue , Gatos/fisiologia , Cães/sangue , Cães/fisiologia , Animais , Animais Recém-Nascidos , Análise Química do Sangue/veterinária , Gatos/crescimento & desenvolvimento , Cães/crescimento & desenvolvimento , Valores de Referência
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...