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1.
Wiad Lek ; 74(3 cz 1): 423-428, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33813444

RESUMO

OBJECTIVE: The aim of the research was to study the cellular composition of the gums in children of primary school age with normal body weight and overweight for further use of this data in the early diagnostics of periodontal diseases. PATIENTS AND METHODS: Materials and methods: We examined 81 children aged from 6 to 12 years. Cytological examination of gingival cytograms was performed in all examined children. RESULTS: Results: Based on the analysis of the quantitative content of epithelial cells in children with normal body weight, their ratio was established, which is determined by the percentage of 0: 6: 94 (parabasal, intermediate, superficial). The obtained data completely coincide with the percentage of the differentiated ratio of epitheliocytes of multilayered squamous epithelium in children with normal body weight with inflammation and without it in the periodontal tissues. Our cytological examinations of gingival scrape smears in overweight children in contrast to the results of the study of epithelial scrape smears in children with normal body weight have some differences. Thus, in the process of calculation, the degree of differentiation of various epitheliocytes determines their percentage as follows - 3: 7: 90 (parabasal, intermediate, superficial) for children with gingivitis, and 2: 5: 93 (parabasal, intermediate, superficial) for children without inflammation in the periodontal tissues. CONCLUSION: Conclusions: The obtained results allowed us to conclude that in overweight children, in contrast to children with normal body weight, the number of parabasal cells decreases, and the number of superficial and intermediate cells increases.


Assuntos
Peso Corporal , Gengiva , Sobrepeso , Índice de Massa Corporal , Criança , Feminino , Gengiva/citologia , Gengiva/patologia , Humanos , Peso Corporal Ideal , Masculino , Sobrepeso/epidemiologia , Instituições Acadêmicas
2.
Med Sci Monit ; 27: e929684, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33690263

RESUMO

The treatment measures of medication-related osteonecrosis of the jaw (MRONJ) is a worldwide challenge in oral and maxillofacial surgery because of its unclear pathogenesis. Previous studies suggested that mesenchymal stem cells played important roles in promoting MRONJ lesion healing, but the detailed mechanisms were unknown. Increasing numbers of studies have demonstrated that exosomes derived from mesenchymal stem cells, especially adipose-derived stem cells, have key roles in stem cell-based therapies by accelerating bone remodeling, facilitating angiogenesis, and promoting wound healing. We hypothesized that exosomes derived from adipose-derived stem cells can prevent MRONJ by accelerating gingival healing and enhancing bone remodeling processes. Our results may provide a promising therapeutic option for MRONJ clinical therapy.


Assuntos
Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/metabolismo , Osteonecrose da Arcada Osseodentária Associada a Difosfonatos/prevenção & controle , Exossomos/transplante , Adipócitos/patologia , Tecido Adiposo/patologia , Remodelação Óssea/fisiologia , Exossomos/metabolismo , Exossomos/patologia , Gengiva/patologia , Humanos , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/patologia , Cicatrização/fisiologia
4.
Int J Mol Sci ; 22(3)2021 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-33513808

RESUMO

There is little known about the effect of the periodontopathogen Filifactor alocis on macrophages as key cells of the innate immune defense in the periodontium. Therefore, the aim of the present study was to investigate the effect of F. alocis and additionally of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNFα) on visfatin and other pro-inflammatory and proteolytic molecules associated with periodontitis in human macrophages. The presence of macrophage markers CD14, CD86, CD68, and CD163 was examined in gingival biopsies from healthy individuals and periodontitis patients. Human macrophages were incubated with F. alocis and TNFα for up to 2 d. The effects of both stimulants on macrophages were determined by real-time PCR, ELISA, immunocytochemistry, and immunofluorescence. F. alocis was able to significantly stimulate the synthesis of visfatin by human macrophages using TLR2 and MAPK pathways. In addition to visfatin, F. alocis was also able to increase the synthesis of cyclooxygenase 2, TNFα, and matrix metalloproteinase 1. Like F. alocis, TNFα was also able to stimulate the production of these proinflammatory and proteolytic molecules. Our results highlight the pathogenetic role of F. alocis in periodontal diseases and also underline the involvement of visfatin in the aetiopathogenesis of periodontitis.


Assuntos
Clostridiales/imunologia , Gengiva/metabolismo , Macrófagos/metabolismo , Nicotinamida Fosforribosiltransferase/biossíntese , Periodontite/imunologia , Receptor 2 Toll-Like/metabolismo , Fator de Necrose Tumoral alfa/farmacologia , Antígenos CD/genética , Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/genética , Antígenos de Diferenciação Mielomonocítica/metabolismo , Antígeno B7-2/genética , Antígeno B7-2/metabolismo , Ciclo-Oxigenase 2/biossíntese , Ciclo-Oxigenase 2/genética , Ciclo-Oxigenase 2/metabolismo , Gengiva/citologia , Gengiva/patologia , Humanos , Imuno-Histoquímica , Receptores de Lipopolissacarídeos/genética , Receptores de Lipopolissacarídeos/metabolismo , Sistema de Sinalização das MAP Quinases/imunologia , Macrófagos/efeitos dos fármacos , Metaloproteinase 1 da Matriz/biossíntese , Metaloproteinase 1 da Matriz/genética , Metaloproteinase 1 da Matriz/metabolismo , Nicotinamida Fosforribosiltransferase/genética , Nicotinamida Fosforribosiltransferase/metabolismo , Periodontite/metabolismo , Periodontite/microbiologia , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Fator de Necrose Tumoral alfa/biossíntese , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
5.
Methods Mol Biol ; 2210: 215-224, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32815142

RESUMO

Porphyromonas gingivalis is a major pathogen responsible for severe and chronic manifestations of periodontal disease, which is one of the most common infectious disorders of humans. Although human gingival epithelium prevents intrusions by periodontal bacteria, P. gingivalis is able to invade gingival epithelial cells. To study the dynamics and the fate of intracellular P. gingivalis, confocal laser scanning microscopy (CLSM) is a method of choice. Information gained with CLSM contains not only the number of P. gingivalis associated with gingival epithelial cells but also the bacterial localization on/inside the host cells, morphological change of host cells, and physical interaction between the bacteria and host organelle. In this chapter, we describe the protocols for microscopy techniques to morphologically study gingival epithelial cells infected by P. gingivalis.


Assuntos
Infecções por Bacteroidaceae/patologia , Células Epiteliais/patologia , Gengiva/patologia , Doenças Periodontais/patologia , Porphyromonas gingivalis/fisiologia , Infecções por Bacteroidaceae/microbiologia , Técnicas de Cultura de Células/métodos , Linhagem Celular , Células Epiteliais/microbiologia , Gengiva/citologia , Gengiva/microbiologia , Humanos , Microscopia Confocal/métodos , Doenças Periodontais/microbiologia , Porphyromonas gingivalis/isolamento & purificação , Coloração e Rotulagem/métodos
7.
Niger J Clin Pract ; 23(10): 1483-1486, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33047710

RESUMO

Metastatic lesions represent approximately 1% of all the intraoral lesions. They most commonly originate from lung and breast carcinomas, while the third most common source is the renal cell carcinoma. In this paper, we present the rare case of metastases of renal cell carcinoma in the mandibular gingiva of a 53-year-old male patient.


Assuntos
Carcinoma de Células Renais/secundário , Gengiva/patologia , Neoplasias Gengivais/secundário , Neoplasias Renais/patologia , Dor nas Costas , Biópsia , Carcinoma de Células Renais/patologia , Neoplasias Gengivais/patologia , Cabeça/diagnóstico por imagem , Humanos , Rim/patologia , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada Multidetectores , Pescoço/diagnóstico por imagem
9.
Braz Oral Res ; 34: e038, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32374812

RESUMO

The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), two major cytokines associated to survival, differentiation and maturation of B cells in biopsies from gingival tissue with periodontitis. Gingival biopsies were obtained from subjects with periodontitis (n = 17), with periodontitis and DM (n = 19) as well as from periodontally and systemically healthy controls (n = 10). Gene expressions for APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were evaluated using qPCR. The expressions APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were all higher in both periodontitis groups when compared to the control group (p < 0.05). Furthermore, the expressions of BLyS, TRAP and RANKL were significantly higher in the subjects with periodontitis and DM when compared to those with periodontitis alone (p < 0.05). The mRNA levels of BLyS correlated positively with RANKL in the subjects with periodontitis and DM (p < 0.05). BLyS is overexpressed in periodontitis tissues of subjects with type 2 DM, suggesting a possible role of this cytokine on the pathogenesis DM-related periodontitis.


Assuntos
Fator Ativador de Células B/análise , Diabetes Mellitus Tipo 2/complicações , Periodontite/imunologia , Periodontite/patologia , Adulto , Idoso , Biomarcadores/análise , Biópsia , Estudos de Casos e Controles , Citocinas/análise , Citocinas/fisiologia , Diabetes Mellitus Tipo 2/imunologia , Feminino , Expressão Gênica , Gengiva/imunologia , Gengiva/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Osteogênese/imunologia , RNA Mensageiro/análise , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Estatísticas não Paramétricas , Membro 13 da Superfamília de Ligantes de Fatores de Necrose Tumoral/análise
10.
BMC Oral Health ; 20(1): 116, 2020 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-32299404

RESUMO

BACKGROUND: The biologic width is defined as the coronal dimension to the alveolar bone that is occupied by healthy gingival tissue. The objective of the present study was to correlate radiographic findings of biologic width invasion with the periodontium status. METHODS: It were included 14 patients with restored teeth with biological width invasion, on the proximal sites, observed clinically and radiographically. 122 proximal sites were evaluated, 61 in the test group (biological width invasion) and 61 in the control group (adequate biological width). Smokers and patients presenting periodontal disease or restorations with contact in eccentric movements, horizontal over-contour or secondary caries were excluded from the sample. The invasion of the biologic width was diagnosed when the distance from the gingival margin of restoration to the bony crest was less than 3 mm. Intrabony defect and bone crest level, as well as, their vertical and horizontal components were radiographically evaluated when present. Plaque index, bleeding on probing, probing depth, gingival recession height, keratinized gingival height and thickness, and clinical attachment level were clinically evaluated. Data were subjected to Spearman's Correlation and Wilcoxon's test. RESULT: The most prevalent tooth with biological width invasion was the first molar. There was a statistically significant correlation between the bone crest (p < 0.001), vertical (p < 0.001) and horizontal (p = 0.001) components. In the test group, there was a statistically significant correlation between bleeding on probing (p < 0.001; r = 0.618) and width of gingival recession (p = 0.030; r = - 0.602) with the intraosseous component; and between keratinized gingival height and bone level (p = 0.037; r = - 0.267). In the control group, there was a correlation between plaque index (p = 0.027; r = - 0.283) with bone level and correlation between keratinized gingival thickness and bone level (p = 0.034; r = - 0.273) and intrabony component (p = 0.042; r = 0.226). CONCLUSION: A statistically significant relationship was found between bleeding on probing and gingival recession in patients who presented intrabony defects due to the invasion of biological width, which may be also related to the thickness of the keratinized gingiva.


Assuntos
Perda do Osso Alveolar/diagnóstico por imagem , Produtos Biológicos , Retração Gengival/diagnóstico por imagem , Periodonto/patologia , Adulto , Perda do Osso Alveolar/etiologia , Estudos de Casos e Controles , Índice de Placa Dentária , Feminino , Seguimentos , Gengiva/diagnóstico por imagem , Gengiva/patologia , Retração Gengival/etiologia , Retração Gengival/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Perda da Inserção Periodontal/diagnóstico por imagem , Perda da Inserção Periodontal/etiologia , Periodontite/diagnóstico por imagem , Periodontite/etiologia , Prevalência , Radiografia
12.
Arch Oral Biol ; 112: 104652, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32114252

RESUMO

OBJECTIVE: To ascertain the role of inducible nitric oxide synthase (iNOS) in the periodontitis response during diabetes. METHODS: Twenty-four male SD rats were randomly divided into four groups: control group (Control), diabetes mellitus group (D), diabetes mellitus plus periodontitis group (DP), and periodontitis group (P). Periodontitis and diabetes were established separately. Then the gingival tissue and alveolar bone were collected. A stereomicroscope was used to evaluate bone loss. The expression of iNOS, TNF-α, and NF-κB in the gingiva was detected by immunohistochemical staining, real-time PCR, and western blot analysis. RESULTS: Significant bone loss was observed in the DP and P groups and more extensive bone resorption was discovered in the DP group than in the P group (P < 0.05). The immunohistochemical staining analysis revealed enhanced expression of iNOS located in the gingiva of the three disease groups compared with the control group (P < 0.05). In particular, the level of iNOS was significantly higher in the DP group than in the P group (P < 0.05). This elevated trend of iNOS was further demonstrated by quantitative PCR and western blot analysis. Similarly, the mRNA and protein expression levels of NF-κB in the D, DP, and P groups were significantly higher than those of the control group, as was the level of TNF-α protein (P < 0.05). CONCLUSIONS: Our results proved diabetes exacerbated alveolar bone resorption in a periodontitis rat model. iNOS may be the inflammatory mediator in the course of periodontal injury promoted by diabetes.


Assuntos
Diabetes Mellitus/metabolismo , Gengiva/metabolismo , Óxido Nítrico Sintase Tipo II/metabolismo , Periodontite/metabolismo , Perda do Osso Alveolar , Animais , Gengiva/patologia , Masculino , Ratos , Ratos Sprague-Dawley
13.
Lasers Med Sci ; 35(5): 1205-1212, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32030556

RESUMO

The aim of this study was to assess the effects of IL-6 and IL-8 cytokines on human gingival fibroblasts (HGF) cultured in a 3-D model and the possible photobiomodulation (PBM) of such effects by low-level laser therapy. In complete culture medium (DMEM), HGF from a healthy patient were seeded in a type I collagen matrix inserted into 24-well plates. After 5 days of incubation, the cytokines were added or not to serum-free DMEM, which was applied to the cell-enriched matrices. Then, PBM was performed: three consecutive irradiations using LaserTable diode device (780 nm, 0.025 W) at 0.5 J/cm2 were delivered or not to the cells. Twenty-four hours after the last irradiation, cell viability and morphology, gene expression, and synthesis of inflammatory cytokines and growth factors were assessed. The histological evaluation demonstrated that, for all groups, matrices presented homogeneous distribution of cells with elongated morphology. However, numerous cytokine-exposed cells were rounded. IL-6 and IL-8 decreased cell viability, synthesis of VEGF, and gene expression of collagen type I. PBM enhanced cell density in the matrices and stimulated VEGF expression, even after IL-6 challenge. Reduced TNF-α synthesis occurred in those cells subjected to PBM. In conclusion, PBM can penetrate collagen matrix and stimulate HGF, highlighting the relevance of this research model for further phototherapy studies and in vitro biomodulation of the healing process.


Assuntos
Técnicas de Cultura de Células/métodos , Citocinas/metabolismo , Fibroblastos/patologia , Fibroblastos/efeitos da radiação , Gengiva/patologia , Inflamação/patologia , Terapia com Luz de Baixa Intensidade , Modelos Biológicos , Sobrevivência Celular/efeitos da radiação , Colágeno Tipo I/metabolismo , Regulação da Expressão Gênica/efeitos da radiação , Humanos , Interleucina-1beta/biossíntese , Fator de Necrose Tumoral alfa/biossíntese , Fator A de Crescimento do Endotélio Vascular/biossíntese , Cicatrização/efeitos da radiação
14.
Braz Oral Res ; 34: e012, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049112

RESUMO

Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.


Assuntos
Lipopeptídeos/farmacologia , Periodontite/etiologia , Periodontite/patologia , Receptor 2 Toll-Like/antagonistas & inibidores , Perda do Osso Alveolar/etiologia , Perda do Osso Alveolar/patologia , Processo Alveolar/efeitos dos fármacos , Processo Alveolar/patologia , Animais , Modelos Animais de Doenças , Gengiva/efeitos dos fármacos , Gengiva/patologia , Gengivite/etiologia , Gengivite/patologia , Masculino , Camundongos Endogâmicos C57BL , Osteoclastos/efeitos dos fármacos , Osteoclastos/fisiologia , Periodontite/microbiologia , Distribuição Aleatória , Fosfatase Ácida Resistente a Tartarato , Fatores de Tempo , Microtomografia por Raio-X
15.
Sci Rep ; 10(1): 3008, 2020 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-32080300

RESUMO

Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gingivitis study. Elastic net regression with stability selection identified a specific metabolite signature in a training dataset that was associated with the observed re-epithelialization kinetics and enabled its prediction for all saliva samples obtained in the clinical study. This signature encompassed ten metabolites, including plasmalogens, diacylglycerol and amino acid derivatives, which reflect enhanced host-microbe interactions. This association is in agreement with the positive correlation of the metabolite signature with the individual's gingival bleeding index. Remarkably, intra-individual signature-variation over time was associated with elevated risk for gingivitis development. Unravelling how these metabolites stimulate wound repair could provide novel avenues towards therapeutic approaches in patients with impaired wound healing capacity.


Assuntos
Eritritol/uso terapêutico , Gengiva/efeitos dos fármacos , Gengivite/metabolismo , Hemorragia/metabolismo , Metaboloma , Saliva/metabolismo , Adolescente , Adulto , Aminoácidos/metabolismo , Aminoácidos/farmacologia , Bioensaio , Estudos de Casos e Controles , Linhagem Celular , Diglicerídeos/metabolismo , Diglicerídeos/farmacologia , Suscetibilidade a Doenças , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Feminino , Gengiva/metabolismo , Gengiva/microbiologia , Gengiva/patologia , Gengivite/tratamento farmacológico , Gengivite/microbiologia , Gengivite/patologia , Hemorragia/tratamento farmacológico , Hemorragia/microbiologia , Hemorragia/patologia , Interações Hospedeiro-Patógeno , Humanos , Masculino , Pessoa de Meia-Idade , Plasmalogênios/metabolismo , Plasmalogênios/farmacologia , Reepitelização/efeitos dos fármacos , Reepitelização/fisiologia , Saliva/química , Saliva/microbiologia , Índice de Gravidade de Doença , Streptococcus mutans/crescimento & desenvolvimento , Streptococcus mutans/patogenicidade
16.
J Oral Pathol Med ; 49(5): 404-408, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31990082

RESUMO

OBJECTIVE: The aim of this systematic review was to gather the available evidence about proliferative verrucous leukoplakia. This systematic review was conducted to answer the question: "What are the main clinical features of PVL patients?". MATERIAL AND METHODS: A systematic review of the literature was done following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statements. An electronic research was carried out using different electronic databases; PubMed, and Scopus. Inclusion criteria were papers which reported at least 10 patients with proliferative verrucous leukoplakia and were published not more than 10 years ago. RESULTS: A total of 285 records were identified through databases, although only 6 of them were eligible. Most patients were women, above 60 years of age (67.4). Additionally, 28 of them were non-smokers (66.6%) and 24 were non-habitual drinkers (57.1%). The most common locations were gingiva (50.9%), buccal mucosa (44.9%), and tongue (40.6%) CONCLUSION: Level C can be established to conclude the proliferative verrucous leukoplakia demographic data, risk factors, malignant transformation, and location. A strict follow-up on these patients should be mandatory, even after surgical management since they have a high recurrence rate and a malignant transformation.


Assuntos
Carcinoma Verrucoso/diagnóstico , Leucoplasia Oral/diagnóstico , Carcinoma Verrucoso/patologia , Transformação Celular Neoplásica , Gengiva/patologia , Humanos , Leucoplasia Oral/patologia , Mucosa Bucal/patologia , Língua/patologia
17.
Niger J Clin Pract ; 23(1): 46-53, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31929206

RESUMO

Objective: Periodontitis and peri-implantitis are irreversible destructive diseases of periodontal and peri-implant tissues. This study aimed to determine the receptor expressions of peroxisome proliferative-activated receptor (PPAR)-γ, retinoid X receptor (RXR)-α, vitamin D receptor (VDR), and cyclooxygenase (COX)-2 in diseased tissues around teeth and dental implants. Subjects and Methods: The study consisted of three groups: group 1, healthy group (C, n = 15); group 2, periodontitis patients with stage 3 grade B (P, n = 15); and group 3, peri-implantitis patients (PI, n = 15). Periodontal clinical parameters such as the plaque index (PI), gingival index (GI), and probing pocket depths (PPD) were recorded. Gingival and peri-implant mucosal biopsies were obtained from all participants and biopsy samples underwent histological tissue processing. Hematoxylin-eosin (H & E) and immunohistochemistry staining were performed. Total inflammatory cell counts and fibroblast cell density were evaluated on H and E-stained slides, while PPAR-γ, RXR-α, VDR, and COX-2 were evaluated through immunohistochemistry. Results: The age of participants were similar, while PI, GI, and PPD values were higher in periodontitis and peri-implantitis groups compared with healthy group. Inflammatory cell infiltration was higher in periodontitis and peri-implantitis compared with healthy group, while fibroblast cell density exhibited a reverse pattern. PPAR-γ and also COX-2 expressions were lower in the healthy group and higher in periodontitis and peri-implantitis groups. RXR-α and VDR were higher in the healthy group and lower in the periodontitis and peri-implantitis groups. Conclusion: The results revealed that RXR-α and VDR levels were higher, while PPAR-γ and COX-2 levels were lower in the healthy group and periodontitis and peri-implantitis groups resulting in similar expressions in the tested parameters.


Assuntos
Ciclo-Oxigenase 2/metabolismo , Gengiva/patologia , PPAR gama/metabolismo , Peri-Implantite/metabolismo , Periodontite/metabolismo , Receptores de Calcitriol/metabolismo , Receptores X de Retinoides/metabolismo , Dente/patologia , Adulto , Estudos de Casos e Controles , Estudos Transversais , Implantes Dentários , Índice de Placa Dentária , Feminino , Gengiva/metabolismo , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Dente/metabolismo
18.
Nanomedicine (Lond) ; 15(1): 55-75, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31868110

RESUMO

Background: Oral cancer is highly aggressive due to difficult diagnosis, therapy resistance and increasing frequency; thus finding prevention therapies is very important. Aim: This study evaluates the use of gold and silver nanoparticles (NPs), phyto-synthesized with Cornus mas extract against oral dysplastic lesions. Methods: NPs were characterized by UV-Vis, Fourier-transform infrared spectroscopy, transmission electron microscopy, x-ray diffraction and laser Doppler microelectrophoresis. Biological testing employed two human oral cell lines: gingival fibroblasts and dysplastic keratinocytes and evaluated viability, cell death mechanisms and cellular uptake. Results: NPs induced selective toxic effects against dysplastic cells. p53/BAX/BCL2 activation and PI3K/AKT inhibition led to cell death through necrosis and apoptosis. NPs also induced antioxidant and anti-inflammatory effects. Conclusion: NPs of gold and silver showed promising beneficial effects in the therapy of oral dysplasia.


Assuntos
Cornus/química , Nanopartículas Metálicas/química , Neoplasias Bucais/tratamento farmacológico , Extratos Vegetais/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Gengiva/efeitos dos fármacos , Gengiva/patologia , Ouro/química , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/patologia , Neoplasias Bucais/genética , Neoplasias Bucais/patologia , Extratos Vegetais/química , Proteínas Proto-Oncogênicas c-bcl-2/genética , Prata/química , Proteína Supressora de Tumor p53/genética , Raios Ultravioleta , Proteína X Associada a bcl-2/genética
19.
J Invest Surg ; 33(1): 49-58, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29856665

RESUMO

Aim: The aim of this study was to evaluate the effect of Hypericum perforatum (HP) oil on wound-healing process in rabbit palatal mucosa. Materials and Methods: Thirty-six New Zealand albino rabbits were randomly allocated to following groups; (1) HP oil (test, n = 18) and (2) olive oil (control, n = 18). Palatinal excisional wounds were created and the oils were topically applied (0.1 ml, 30 s, twice a day). Gingival biopsies were excised, and analyzed for re-epithelialization (RE) and granulation tissue maturation (GTM) on days 3, 7, and 14 after surgery. Levels of vascular endothelial growth factor (VEGF) and fibroblast growth factor 2 (FGF-2) were assessed using the immunohistochemical method. Apoptotic cells (ACs) were evaluated using TUNEL staining. Enzyme-linked immunosorbent assay was used to assess tissue catalase (CAT) and malondialdehyde (MDA) levels. Results: RE and GTM were completed earlier in the HP oil group than in the control group. The number of positively stained cells/vessels was higher in olive oil than in the test group on day 3 for FGF-2 and on days 3 and 7 for VEGF (p < 0.05). In contrast, on day 14, a higher number of vessels was observed in the HP oil group than in the control group. HP oil treatment reduced the number of ACs compared to olive oil (p < 0.05), but the difference during the healing period did not reach significance. Tissue CAT and MDA levels between groups were not different, and also the results were the same when the levels were analyzed by the evaluated time periods (p > 0.05). Conclusions: The results of this study demonstrated that topical HP oil treatment did not provide an additional benefit to its base, olive oil, in the early phase of secondary wound healing.


Assuntos
Hypericum/química , Mucosa Bucal/efeitos dos fármacos , Óleos Vegetais/administração & dosagem , Reepitelização/efeitos dos fármacos , Ferida Cirúrgica/tratamento farmacológico , Administração Tópica , Animais , Biópsia , Modelos Animais de Doenças , Gengiva/efeitos dos fármacos , Gengiva/patologia , Gengiva/cirurgia , Humanos , Masculino , Mucosa Bucal/cirurgia , Palato/efeitos dos fármacos , Palato/patologia , Palato/cirurgia , Coelhos
20.
Lasers Med Sci ; 35(1): 31-40, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30341668

RESUMO

This study was designed to investigate the effect of 650-nm low-level laser irradiation (LLLI) as an adjunctive treatment of experimental periodontitis. To investigate possible LLLI-mediated anti-inflammatory effects, we utilized an experimental periodontitis (EP) rat model and analyzed c-Jun, c-Fos, ICAM-1, and CCL2 gene expressions on PB leukocytes and in the gingival tissue. Total RNA was isolated from the gingivae and peripheral blood (PB) leukocytes of normal, EP, scaling, and root planing (SRP)-treated EP and LLLI + SRP-treated EP rats, and gene expressions were analyzed by real-time PCR. The productions of c-Jun, c-Fos, ICAM-1, and CCL2 in gingivae were analyzed immunohistochemically. Tartrate-resistant acid phosphatase (TRAP) staining was used to determine osteoclast activity in alveolar bone. The c-Jun and ICAM-1 messenger RNA (mRNA) levels were significantly decreased in the EP rat gingival tissue treated by SRP + LLLI than by SRP, the c-Jun, ICAM-1, and c-Fos mRNA levels on PB leukocytes reduced after LLLI treatment but did not show any significant differences in both groups. There was no significant difference in CCL2 mRNA levels on PB leukocytes and in gingivae between the SRP + LLLI and the SRP groups. The c-Fos mRNA levels in gingivae did not show significant difference in both groups. Immunohistochemistry showed that the CCL2, ICAM-1, c-Jun, and c-Fos productions were significantly reduced in rats of the SRP + LLLI group compared with the only SRP group. LLLI significantly decreased the number of osteoclasts as demonstrated by TRAP staining. The 650-nm LLLI might be a useful treatment modality for periodontitis.


Assuntos
Quimiocina CCL2/metabolismo , Molécula 1 de Adesão Intercelular/metabolismo , Terapia com Luz de Baixa Intensidade , Periodontite/metabolismo , Periodontite/radioterapia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Proteínas Proto-Oncogênicas c-jun/metabolismo , Animais , Quimiocina CCL2/genética , Regulação da Expressão Gênica , Gengiva/metabolismo , Gengiva/patologia , Molécula 1 de Adesão Intercelular/genética , Masculino , Osteoclastos/patologia , Osteoclastos/efeitos da radiação , Periodontite/genética , Periodontite/patologia , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-jun/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos Sprague-Dawley
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