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1.
Medicine (Baltimore) ; 99(29): e20542, 2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32702812

RESUMO

BACKGROUND: This study will investigate the clinical efficacy of Duyiwei capsule (DYWC) for the treatment of gingivitis. METHODS: Relevant studies will be searched in PUBMED, EMBASE, Cochrane Library, WANGFANG, VIP, CBM, and CNKI from inception to the March 31, 2020 without limitations of language and publication time. All potential randomized controlled trials on the clinical efficacy of DYWC for the treatment of gingivitis will be considered. Two authors will independently perform literature selection, data collection, and study quality assessment. Any disagreements will be solved by a third author through discussion. We will utilize RevMan 5.3 software for statistical analysis. RESULTS: This study will summarize present randomized controlled trials on the efficacy and safety of DYWC for the treatment of gingivitis. CONCLUSION: The findings of this study will provide evidence to show whether DYWC is effective and safety for gingivitis.Systematic review registration: INPLASY202040199.


Assuntos
Gengivite/tratamento farmacológico , Gengivite/patologia , Medicina Tradicional Chinesa/métodos , Feminino , Humanos , Masculino , Medicina Tradicional Chinesa/efeitos adversos , Garantia da Qualidade dos Cuidados de Saúde/métodos , Ensaios Clínicos Controlados Aleatórios como Assunto , Projetos de Pesquisa , Resultado do Tratamento
2.
J Appl Oral Sci ; 28: e20190490, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32267379

RESUMO

BACKGROUND: The relationship between periodontitis and the pathogenesis of other inflammatory diseases, such as diabetes, rheumatoid arthritis and obesity has been an important topic of study in recent decades. The Th17 pathway plays a significant role in how local inflammation can influence systemic inflammation in the absence of systemic pathology. OBJECTIVE: To determine Th17 biased-cells in systemically healthy patients in the presence of generalized chronic periodontitis. METHODOLOGY: A total of 28 patients were recruited without systemic inflammatory pathology, which was determined by clinical history, the Health Assessment Questionnaire (HAQ) and rheumatoid factor detection. Of these patients, 13 were diagnosed as healthy/gingivitis (H/G) and 15 as generalized chronic periodontitis (GCP). Th17 (CD4+CD161+) cells and Th17IL23R+ (CD4+CD161+IL-23R+) cells were quantified by flow cytometry, based on the total cells and on the lymphocyte region, termed the "enriched population" (50,000 events for each). RESULTS: The percentages of Th17 cells of the H/G and periodontitis groups were similar on total cells and enriched population (19 vs 21.8; p=4.134 and 19.6 vs 21.8; p=0.55). However, Th17IL23R+ cells differ significantly between periodontally healthy patients and generalized chronic periodontitis patients in both total cell (0.22% vs 0.65%; p=0.0004) and enriched populations (0.2% vs 0.75%; p=0.0266). CONCLUSIONS: GCP patients (otherwise systemically healthy) were characterized by increased Th17-proinflammatory cell phenotype positive for the IL-23 receptor in peripheral blood. The proportion of Th17 cells that are negative for the IL-23 receptor in the peripheral blood of systemically healthy patients seemed to be unaffected by the presence or absence of chronic periodontitis.


Assuntos
Periodontite Crônica/imunologia , Células Th17/imunologia , Adulto , Idoso , Estudos de Casos e Controles , Periodontite Crônica/patologia , Feminino , Citometria de Fluxo , Gengivite/imunologia , Gengivite/patologia , Humanos , Interleucina-23/sangue , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Fenótipo , Receptores de Interleucina/sangue , Estatísticas não Paramétricas , Inquéritos e Questionários , Células Th17/patologia , Adulto Jovem
3.
Braz Oral Res ; 34: e015, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32130362

RESUMO

We sought to compare the characteristics and clinical significance of neutrophil extracellular traps in gingival samples from patients with periodontitis and those with gingivitis. The clinical indexes of gingival samples from patients with periodontitis and gingivitis were measured; the expression of TNF-alpha and IL-8 was measured by real-time fluorescent quantitative PCR; and the expression of TLR-8 and MMP-9 was measured by western blotting assays. Chemotaxis, phagocytosis and phagocytic activity of neutrophils were measured. Compared with the healthy group, the expression of TNF-α and IL-8 in the periodontitis group and the gingivitis group increased significantly (p < 0.05), and TNF-α in the gingivitis group was significantly lower than that in the healthy group (p < 0.05). The expression of IL-8 in the periodontitis group was significantly higher than that in the periodontitis group (p < 0.05). Furthermore, the expression of TLR-8 and MMP-9 in the periodontitis group was different from that in the gingivitis group and the healthy group, and the expression of TLR-8 and MMP-9 in the gingivitis group was significantly different from that in the healthy group (p < 0.05). In addition, the neutrophil mobility index in healthy people was 3.02 ± 0.53, that in the periodontitis group was 2.21 ± 0.13, and that in the gingivitis group was 2.31 ± 0.12. In conclusion, the chemotaxis of neutrophils in gingival samples of patients with periodontitis and gingivitis was decreased, the phagocytotic ability and activity of neutrophils were reduced, and the release of the extracellular trap-releasing inducible factors TNF-alpha and IL-8 also declined.


Assuntos
Armadilhas Extracelulares , Gengivite/patologia , Neutrófilos/patologia , Periodontite/patologia , Actinas/análise , Adulto , Western Blotting , Estudos de Casos e Controles , Eletroforese em Gel de Ágar , Feminino , Humanos , Interleucina-8/análise , Masculino , Metaloproteinase 9 da Matriz/análise , Pessoa de Meia-Idade , Índice Periodontal , RNA/análise , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Receptor 8 Toll-Like/análise , Fator de Necrose Tumoral alfa/análise , Adulto Jovem
4.
J Oral Pathol Med ; 49(5): 443-449, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32133698

RESUMO

BACKGROUND: Reports of oral manifestations of granulomatosis with polyangiitis (GPA) usually refer to single-case reports; "strawberry gingivitis" has been increasingly reported. OBJECTIVE: To study the clinicopathological findings of four patients in which the diagnosis of GPA was suspected from the observation of their oral lesions and compare these to existent data. METHODS: Retrospective study of a case series. RESULTS: One patient presented typical "strawberry gingivitis" with localized disease and negative ANCA results. Two patients presented rapidly growing oral ulcers associated with systemic compromise and high ANCA levels. One patient presented with a chronic granulomatous lesion that leaded to palatal perforation. CONCLUSION: Oral manifestations of GPA may vary from rapidly evolving lesions in acutely ill patients to chronic and locally destructive lesions in slowing developing disease. These differences are also evident in the histopathological findings.


Assuntos
Gengivite/patologia , Granulomatose com Poliangiite/patologia , Palato/patologia , Anticorpos Anticitoplasma de Neutrófilos/sangue , Gengivite/etiologia , Granulomatose com Poliangiite/complicações , Humanos , Estudos Retrospectivos
5.
Braz Oral Res ; 34: e012, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049112

RESUMO

Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.


Assuntos
Lipopeptídeos/farmacologia , Periodontite/etiologia , Periodontite/patologia , Receptor 2 Toll-Like/antagonistas & inibidores , Perda do Osso Alveolar/etiologia , Perda do Osso Alveolar/patologia , Processo Alveolar/efeitos dos fármacos , Processo Alveolar/patologia , Animais , Modelos Animais de Doenças , Gengiva/efeitos dos fármacos , Gengiva/patologia , Gengivite/etiologia , Gengivite/patologia , Masculino , Camundongos Endogâmicos C57BL , Osteoclastos/efeitos dos fármacos , Osteoclastos/fisiologia , Periodontite/microbiologia , Distribuição Aleatória , Fosfatase Ácida Resistente a Tartarato , Fatores de Tempo , Microtomografia por Raio-X
6.
Sci Rep ; 10(1): 3008, 2020 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-32080300

RESUMO

Several proteins and peptides in saliva were shown to stimulate gingival wound repair, but the role of salivary metabolites in this process remains unexplored. In vitro gingival re-epithelialization kinetics were determined using unstimulated saliva samples from healthy individuals collected during an experimental gingivitis study. Elastic net regression with stability selection identified a specific metabolite signature in a training dataset that was associated with the observed re-epithelialization kinetics and enabled its prediction for all saliva samples obtained in the clinical study. This signature encompassed ten metabolites, including plasmalogens, diacylglycerol and amino acid derivatives, which reflect enhanced host-microbe interactions. This association is in agreement with the positive correlation of the metabolite signature with the individual's gingival bleeding index. Remarkably, intra-individual signature-variation over time was associated with elevated risk for gingivitis development. Unravelling how these metabolites stimulate wound repair could provide novel avenues towards therapeutic approaches in patients with impaired wound healing capacity.


Assuntos
Eritritol/uso terapêutico , Gengiva/efeitos dos fármacos , Gengivite/metabolismo , Hemorragia/metabolismo , Metaboloma , Saliva/metabolismo , Adolescente , Adulto , Aminoácidos/metabolismo , Aminoácidos/farmacologia , Bioensaio , Estudos de Casos e Controles , Linhagem Celular , Diglicerídeos/metabolismo , Diglicerídeos/farmacologia , Suscetibilidade a Doenças , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Feminino , Gengiva/metabolismo , Gengiva/microbiologia , Gengiva/patologia , Gengivite/tratamento farmacológico , Gengivite/microbiologia , Gengivite/patologia , Hemorragia/tratamento farmacológico , Hemorragia/microbiologia , Hemorragia/patologia , Interações Hospedeiro-Patógeno , Humanos , Masculino , Pessoa de Meia-Idade , Plasmalogênios/metabolismo , Plasmalogênios/farmacologia , Reepitelização/efeitos dos fármacos , Reepitelização/fisiologia , Saliva/química , Saliva/microbiologia , Índice de Gravidade de Doença , Streptococcus mutans/crescimento & desenvolvimento , Streptococcus mutans/patogenicidade
7.
Medicine (Baltimore) ; 99(2): e18338, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914014

RESUMO

BACKGROUND: This study aims to explore the effectiveness of vitamin D for the management of adult patients with gingivitis. METHODS: We will perform a comprehensive search from the following electronic databases: Cochrane Library, PUBMED, EMBASE, AMED, CINAHL, WANGFANG, VIP, CBM, and China National Knowledge Infrastructure. All databases will be searched from their inceptions to the present without language limitation. We will also search for unpublished data to avoid missing more potential studies. Two authors will carry out study selection, data extraction, and methodological quality evaluation, respectively. RevMan 5.3 software will be utilized for statistical analysis. RESULTS: This study will summarize the up-to-date evidence about the anti-inflammatory effect of vitamin D for the management of adult patients with gingivitis through assessing modified gingival, gingival bleeding indices, inflammatory factors, plaque, quality of life, and any adverse events. CONCLUSION: This study may provide helpful evidence of vitamin D for the management of adult patients with gingivitis for clinical practice. SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42019156561.


Assuntos
Gengivite/tratamento farmacológico , Gengivite/psicologia , Vitamina D/uso terapêutico , Adulto , China/epidemiologia , Gengivite/patologia , Humanos , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento , Vitamina D/efeitos adversos
9.
J. appl. oral sci ; 28: e20190490, 2020. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1090781

RESUMO

Abstract The relationship between periodontitis and the pathogenesis of other inflammatory diseases, such as diabetes, rheumatoid arthritis and obesity has been an important topic of study in recent decades. The Th17 pathway plays a significant role in how local inflammation can influence systemic inflammation in the absence of systemic pathology. Objective: To determine Th17 biased-cells in systemically healthy patients in the presence of generalized chronic periodontitis. Methodology: A total of 28 patients were recruited without systemic inflammatory pathology, which was determined by clinical history, the Health Assessment Questionnaire (HAQ) and rheumatoid factor detection. Of these patients, 13 were diagnosed as healthy/gingivitis (H/G) and 15 as generalized chronic periodontitis (GCP). Th17 (CD4+CD161+) cells and Th17IL23R+ (CD4+CD161+IL-23R+) cells were quantified by flow cytometry, based on the total cells and on the lymphocyte region, termed the "enriched population" (50,000 events for each). Results: The percentages of Th17 cells of the H/G and periodontitis groups were similar on total cells and enriched population (19 vs 21.8; p=4.134 and 19.6 vs 21.8; p=0.55). However, Th17IL23R+ cells differ significantly between periodontally healthy patients and generalized chronic periodontitis patients in both total cell (0.22% vs 0.65%; p=0.0004) and enriched populations (0.2% vs 0.75%; p=0.0266). Conclusions: GCP patients (otherwise systemically healthy) were characterized by increased Th17-proinflammatory cell phenotype positive for the IL-23 receptor in peripheral blood. The proportion of Th17 cells that are negative for the IL-23 receptor in the peripheral blood of systemically healthy patients seemed to be unaffected by the presence or absence of chronic periodontitis.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Periodontite Crônica/imunologia , Células Th17/imunologia , Fenótipo , Estudos de Casos e Controles , Índice Periodontal , Inquéritos e Questionários , Receptores de Interleucina/sangue , Estatísticas não Paramétricas , Interleucina-23/sangue , Periodontite Crônica/patologia , Células Th17/patologia , Citometria de Fluxo , Gengivite/imunologia , Gengivite/patologia
10.
Int J Mol Sci ; 20(24)2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31817424

RESUMO

During inflammation of the gums, resident cells of the periodontium, gingival fibroblasts (GFs), interact with heterogeneous cell populations of the innate and adaptive immune system that play a crucial role in protecting the host from pathogenic infectious agents. We investigated the effects of chronic inflammation, by exposing peripheral blood mononuclear cells (PBMCs), peripheral blood lymphocyte (PBL) cultures, and GF-PBMC cocultures to Toll-like receptor 2 (TLR2) and TLR4 activators for 21 days and assessed whether this influenced leukocyte retention, survival, and proliferation. Chronic stimulation of PBMC-GF cocultures with TLR2 and TLR4 agonists induced a reduction of NK (CD56+CD3-), T (CD3+), and B (CD19+) cells, whereas the number of TLR-expressing monocytes were unaffected. TLR2 agonists doubled the T cell proliferation, likely of a selective population, given the net decrease of T cells. Subsequent chronic exposure experiments without GF, using PBMC and PBL cultures, showed a significantly (p < 0.0001) increased proinflammatory cytokine production of TNF-α and IL-1ß up to 21 days only in TLR2-activated PBMC with concomitant T cell proliferation, suggesting a role for monocytes. In conclusion, chronic TLR activation mediates the shift in cell populations during infection. Particularly, TLR2 activators play an important role in T cell proliferation and proinflammatory cytokine production by monocytes, suggesting that TLR2 activation represents a bridge between innate and adaptive immunity.


Assuntos
Proliferação de Células , Fibroblastos/imunologia , Gengiva/imunologia , Gengivite/imunologia , Linfócitos T/imunologia , Receptor 2 Toll-Like/imunologia , Linfócitos B/imunologia , Linfócitos B/patologia , Feminino , Fibroblastos/patologia , Gengiva/patologia , Gengivite/patologia , Humanos , Interleucina-1beta/imunologia , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/patologia , Masculino , Monócitos/imunologia , Monócitos/patologia , Linfócitos T/patologia , Receptor 4 Toll-Like/imunologia , Fator de Necrose Tumoral alfa/imunologia
11.
Rev. esp. patol ; 52(4): 208-213, oct.-dic. 2019. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-191940

RESUMO

INTRODUCTION AND OBJECTIVES: The objective of this study was to observe the evolution of genotoxicity (micronucleation, binucleation and multinucleation) from normal to periodontally compromised gingival epithelium (gingivitis and periodontitis) and to compare the severity of damage. METHODS AND MATERIAL: 45 participants formed 3 different categories; a control group of 15 healthy subjects, 15 subjects with gingivitis and 15 with chronic periodontitis. Smears were collected from all the gingiva and stained with acridine orange stain. A total of 500 cells were evaluated under fluorescent microscopy for nuclear abnormalities such as micronuclei, binucleation and multinucleation. The statistical analysis used was one way ANOVA and posthoc Tukey test. RESULTS AND CONCLUSIONS: A statistically significant difference was observed when the age of the 3 groups were compared (p = 0.002); the control group were younger than those with chronic periodontitis or gingivitis. With respect to genotoxic changes, the differences for binucleation (p = 0.002) and multinucleation (p < 0.001) were statistically significant thus suggesting advanced damage in the nucleus. Such changes in genotoxicity could be of help to a clinician in determining prognosis


INTRODUCCIÓN Y OBJETIVOS: El objetivo de este estudio fue observar las tendencias en cuanto a genotoxicidad (micronucleación, binucleación y multinucleación) a partir de epitelio gingival de normal a periodoncialmente-comprometido (gingivitis y periodontitis), y comparar la gravedad del daño entre ambas situaciones. MÉTODOS Y MATERIAL: Seleccionamos a cuarenta y cinco sujetos, que agrupamos en 3 categorías (15 sujetos sanos, 15 con gingivitis y 15 con periodontitis crónica). Se recogieron frotis del tejido gingival de dichos sujetos, que se tiñeron con acridina naranja. Evaluamos un total de 500 células bajo el microscopio fluorescente en busca de anomalías nucleares de tipo micronúcleos, binucleación y multinucleación. El análisis estadístico utilizado fue ANOVA unidireccional y Tukey posthoc. RESULTADOS Y CONCLUSIÓN: Observamos una diferencia estadísticamente significativa al comparar los 3 grupos de edad (p = 0,002), donde el grupo control (sujetos sanos) pertenecía a un rango de edad inferior a los sujetos con periodontitis o gingivitis crónicas. Con respecto a los cambios genotóxicos, las diferencias para binucleación (p = 0,002) y multinucleación (p < 0,001) fueron estadísticamente significativas, lo cual sugiere un daño avanzado en el núcleo. Las tendencias en cuanto a genotoxicidad podrían ayudar a los clínicos a determinar el pronóstico de la enfermedad


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Gengivite/patologia , Periodontite/patologia , Microscopia de Fluorescência/métodos , Núcleo Celular/patologia , Estudos de Casos e Controles , Laranja de Acridina , Micronúcleo Germinativo/patologia , Estudos Transversais , Genotoxicidade/análise
12.
J Appl Oral Sci ; 27: e20180476, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596364

RESUMO

OBJECTIVES: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proinflammatory cytokines IL-1 α, IL-6, IL-17, and TNF-α and osteoclastogenesis marker RANK, RANKL, and OPG in gingival tissue, using the real-time polymerase chain reaction technique. METHODOLOGY: Thirteen patients of both sexes (11-49 years old) under orthodontic treatment were selected, obtaining 11 successful and 7 failed mini-implants. The mini-implants were placed and removed by the same surgeon, in both jaws. The mean time of permanence in the mouth was 29.4 months for successful and 7.6 months for failed mini-implants. At removal time, peri-mini-implant gingival tissue samples were collected and processed for quantification of the proinflammatory cytokines and osteoclastogenesis markers. Nonparametric Wilcoxon rank-sum test considering the clusters and Kruskal-Wallis test were used for statistical analysis (α=0.05). RESULTS: No significant difference (p>0.05) was observed between the groups for either quantification of cytokines or osteoclastogenesis markers, except for IL-6 (p<0.05). CONCLUSIONS: It may be concluded that the expression of IL-1α, IL-17, TNF-α, RANK, RANKL, and OPG in peri-implant gingival tissue were not determinant for mini-implant stability loss, but the higher IL-6 expression could be associated with mini-implant failure.


Assuntos
Citocinas/análise , Implantes Dentários/efeitos adversos , Gengivite/patologia , Procedimentos de Ancoragem Ortodôntica/efeitos adversos , Osteogênese/fisiologia , Peri-Implantite/patologia , Adolescente , Adulto , Perda do Osso Alveolar , Biomarcadores/análise , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteoprotegerina/análise , Reação em Cadeia da Polimerase em Tempo Real , Valores de Referência , Estatísticas não Paramétricas , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
13.
Rev Esp Patol ; 52(4): 208-213, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31530403

RESUMO

INTRODUCTION AND OBJECTIVES: The objective of this study was to observe the evolution of genotoxicity (micronucleation, binucleation and multinucleation) from normal to periodontally compromised gingival epithelium (gingivitis and periodontitis) and to compare the severity of damage. METHODS AND MATERIAL: 45 participants formed 3 different categories; a control group of 15 healthy subjects, 15 subjects with gingivitis and 15 with chronic periodontitis. Smears were collected from all the gingiva and stained with acridine orange stain. A total of 500 cells were evaluated under fluorescent microscopy for nuclear abnormalities such as micronuclei, binucleation and multinucleation. The statistical analysis used was one way ANOVA and posthoc Tukey test. RESULTS AND CONCLUSION: A statistically significant difference was observed when the age of the 3 groups were compared (p=0.002); the control group were younger than those with chronic periodontitis or gingivitis. With respect to genotoxic changes, the differences for binucleation (p=0.002) and multinucleation (p<0.001) were statistically significant thus suggesting advanced damage in the nucleus. Such changes in genotoxicity could be of help to a clinician in determining prognosis.


Assuntos
Núcleo Celular/patologia , Periodontite Crônica/patologia , Gengivite/patologia , Microscopia de Fluorescência , Laranja de Acridina , Adulto , Estudos Transversais , Feminino , Corantes Fluorescentes , Humanos , Masculino , Micronúcleos com Defeito Cromossômico , Pessoa de Meia-Idade , Testes de Mutagenicidade , Prognóstico , Adulto Jovem
15.
Acta Odontol Latinoam ; 32(1): 17-21, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-31206570

RESUMO

The aim of this study is to establish the prevalence of Chronic Periodontitis (CP) in patients with Chronic Kidney Disease (CKD) and to ascertain its relationship with several factors or indicators of micro inflammation. One hundred and thirty-jive CKD patients on dialysis treatment were included. Biochemical parameters, clinical attachment level and pocket depth were recorded according of the American Academy of Periodontology and the CDC (CDC-AAP). Gingivitis and CP were recorded based on the biofilm-gingival interface (BGI) periodontal diseases classification. The rate of non-response to the survey was 10 percent. A total 2,636 teeth in 135 patients were examined, of whom 52.5% were males. Average age was 55.7 years (SD ± 1.32); 41.4% had a smoking history; 78/135 patients were on hemodialysis and 57/135 on peritoneal dialysis; 55.5% had been on dialysis for more than three years. Prevalence of gingivitis and periodontitis was 14.8%, 95% CI (9.7-21.9) and 82.2%, 95% CI (74.7 - 87.8), respectively; according to the BGI Index. Severity of CP was: No periodontitis, 14.0% 95% CI (9.1 - 21.1); mild, 11.1% 95% CI (6.7 -17.7); moderate, 28.8% 95% CI (21.7- 37.1); and severe, 45.9% 95% CI (31.6-54.47). Peritoneal dialysis and time on dialysis > 3 years increase the chance of having periodontitis, OR 11.0 95% CI (2.2-53.8) and OR 7.6 95% CI (1.1-50.2), respectively. In view of the high prevalence of CP in this population, programs designed to ensure better periodontal and gingival care in the population on dialysis need to be established.


Assuntos
Periodontite Crônica/epidemiologia , Periodontite Crônica/patologia , Gengivite/epidemiologia , Gengivite/patologia , Falência Renal Crônica/complicações , Periodonto/patologia , Proteína C-Reativa/análise , Periodontite Crônica/etiologia , Colômbia/epidemiologia , Diabetes Mellitus/epidemiologia , Feminino , Gengivite/etiologia , Humanos , Falência Renal Crônica/sangue , Falência Renal Crônica/terapia , Masculino , Pessoa de Meia-Idade , Perda da Inserção Periodontal , Índice Periodontal , Prevalência , Diálise Renal , Fumar
16.
Clin Oral Investig ; 23(6): 2651-2658, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30327948

RESUMO

OBJECTIVES: No data are available in the literature on the extent to which the immune host-response and bacterial-elicited inflammation separately contributes to the increase in gingival crevicular fluid (GCF) levels of inflammatory biomarkers in patients affected by desquamative gingivitis (DG) secondary to oral lichen planus (OLP). The aim of this study was to investigate the effect of a structured plaque control intervention on GCF levels of MMP-1 and MMP-9 in OLP patients with DG and to compare them with those of non-OLP patients. MATERIALS AND METHODS: The study population consisted of 18 unrelated Caucasian patients with DG, while 18 periodontally healthy subjects were recruited for the control group. Periodontal parameters and GCF biomarker amounts were evaluated at baseline and 2 months after a structured plaque control intervention, comprising professional oral hygiene sessions, manual toothbrushing, and interdental cleaning advice, only for DG patients. Determination of MMP-1 and MMP-9 levels was carried out by means of an enzyme-linked immunosorbent assay. RESULTS: Plaque control program led to improvement in all examined clinical parameters and resulted in significant decrease in GCF total amount and concentration of MMP-1 and MMP-9 in comparison to baseline (p < 0.001). However, MMP-1 and MMP-9 levels in DG patients were still significantly higher than those in the healthy control group (p < 0.01). CONCLUSIONS: These findings would seem to support an intrinsic upregulated expression of MMPs in DG patients that is exacerbated by bacterial plaque. CLINICAL RELEVANCE: The present outcomes provide further scientific grounds for the importance of strict professional oral hygiene sessions in DG patients.


Assuntos
Placa Dentária/prevenção & controle , Líquido do Sulco Gengival/metabolismo , Gengivite/patologia , Líquen Plano Bucal/patologia , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Idoso , Biomarcadores/metabolismo , Estudos de Casos e Controles , Índice de Placa Dentária , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
17.
J Periodontal Res ; 54(3): 218-224, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30298590

RESUMO

BACKGROUND AND OBJECTIVE: Neutrophil extracellular traps (NETs) are a recently discovered antimicrobial mechanism used by neutrophils that have been proposed as an intervention in the pathogenesis of periodontitis. The objective of our study was to characterize the expression of NETs in gingival tissues with periodontitis and controls and to compare the expression of these traps in gingival tissue samples of patients with gingivitis and periodontitis. MATERIAL AND METHODS: An observational cross-sectional study was conducted on patients with periodontitis, gingivitis, and controls that needed tooth extraction. Gingival tissue biopsies were gathered after clinical examination and tooth extraction. Electron microscopy and immunofluorescence were performed to characterize NETs, comparing periodontitis and control patients. Immunohistochemical analysis was performed to quantify neutrophil extracellular trap expression through extracellular citrullinated histone H3 and myeloperoxidase in biopsies from patients with gingivitis and periodontitis. RESULTS: Thirteen biopsies were gathered from 13 patients: five gingivitis, six periodontitis, and two controls. Electron microscopy and immunofluorescence imaging showed greater expression of neutrophils present in periodontal inflamed tissue compared with controls. Release of nuclear content to the extracellular space was observed, compatible with the formation of NETs. The expression of citrullinated histone H3 was higher in gingivitis samples than periodontitis samples (P = 0.0106). Myeloperoxidase expression was higher in periodontitis than gingivitis, but without achieving statistical significance. CONCLUSION: Neutrophil extracellular traps were found in tissue samples of periodontitis as extracellular components of chromatin, along with neutrophil enzymes, that were not present in healthy controls. The comparison of NETs expression in periodontitis and gingivitis showed higher expression in gingivitis, associating them to acute phases of the periodontal inflammatory process.


Assuntos
Armadilhas Extracelulares , Gengiva/patologia , Gengivite/patologia , Neutrófilos/citologia , Periodontite/patologia , Estudos de Casos e Controles , Cromatina , Estudos Transversais , Gengiva/metabolismo , Gengivite/metabolismo , Histonas/metabolismo , Humanos , Microscopia Eletrônica de Transmissão , Neutrófilos/ultraestrutura , Periodontite/metabolismo , Peroxidase/metabolismo , Projetos Piloto
19.
Oral Dis ; 25(1): 265-273, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30285304

RESUMO

OBJECTIVE: Although accumulating evidence indicates that macrophages are central players in the destructive and reparative phases of periodontal disease, their polarization states at different stages of periodontal inflammation remain unclear. METHODS: We collected gingival biopsies from patients with chronic periodontitis (P group), gingivitis (G group), or periodontally healthy individuals (H group). Polarized macrophages were identified through immunofluorescence. M1- and M2-related cytokines were detected by immunohistochemistry. RESULTS: Compared with the H group, the P group had more M1 cells (higher M1/M2 ratio) and significantly higher TNF-α, IFN-γ, IL-6, and IL-12 levels. Although the G group also exhibited higher TNF-α and IL-12 levels than the H group, they had similar M1/M2 ratios. The M1/M2 ratio and IFN-γ and IL-6 levels were significantly higher in the P than the G group. Among M2-related cytokines, IL-4 levels were significantly higher in the G than the H group. The M1/M2 ratio was positively correlated with clinical probing depth (PD), and both were positively correlated with IFN-γ and IL-6. PD was negatively correlated with IL-4. CONCLUSION: Macrophage polarization in gingival tissue may be responsible for the development and progression of inflammation-induced tissue destruction, and modulating macrophage function may be a potential strategy for periodontal disease management.


Assuntos
Periodontite Crônica/patologia , Gengiva/citologia , Gengivite/patologia , Ativação de Macrófagos , Macrófagos/citologia , Adulto , Estudos de Casos e Controles , Citocinas/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
20.
J. appl. oral sci ; 27: e20180476, 2019. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1040231

RESUMO

Abstract Objectives: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proinflammatory cytokines IL-1 α, IL-6, IL-17, and TNF-α and osteoclastogenesis marker RANK, RANKL, and OPG in gingival tissue, using the real-time polymerase chain reaction technique. Methodology: Thirteen patients of both sexes (11-49 years old) under orthodontic treatment were selected, obtaining 11 successful and 7 failed mini-implants. The mini-implants were placed and removed by the same surgeon, in both jaws. The mean time of permanence in the mouth was 29.4 months for successful and 7.6 months for failed mini-implants. At removal time, peri-mini-implant gingival tissue samples were collected and processed for quantification of the proinflammatory cytokines and osteoclastogenesis markers. Nonparametric Wilcoxon rank-sum test considering the clusters and Kruskal-Wallis test were used for statistical analysis (α=0.05). Results: No significant difference (p>0.05) was observed between the groups for either quantification of cytokines or osteoclastogenesis markers, except for IL-6 (p<0.05). Conclusions: It may be concluded that the expression of IL-1α, IL-17, TNF-α, RANK, RANKL, and OPG in peri-implant gingival tissue were not determinant for mini-implant stability loss, but the higher IL-6 expression could be associated with mini-implant failure.


Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Osteogênese/fisiologia , Implantes Dentários/efeitos adversos , Citocinas/análise , Procedimentos de Ancoragem Ortodôntica/efeitos adversos , Peri-Implantite/patologia , Gengivite/patologia , Valores de Referência , Fatores de Tempo , Biomarcadores/análise , Perda do Osso Alveolar , Resultado do Tratamento , Estatísticas não Paramétricas , Osteoprotegerina/análise , Reação em Cadeia da Polimerase em Tempo Real
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