Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 6.769
Filtrar
1.
Emerg Med Clin North Am ; 37(4): 611-635, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31563198

RESUMO

Genitourinary trauma often occurs concomitantly with other abdominopelvic trauma, but nevertheless is important to master in diagnosis, management, and treatment. There are subtleties to diagnosis and important steps that should not be missed to properly manage patients.


Assuntos
Genitália Feminina/lesões , Genitália Masculina/lesões , Sistema Urinário/lesões , Emergências , Feminino , Humanos , Masculino
2.
J Insect Sci ; 19(3)2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31225878

RESUMO

Males of many Psylloidea are known to possess a characteristic structure at the functional hub of their reproductive apparatus, between afferent and efferent passage of seminal fluid. The structure is a squat, cylindrical endoskeleton consisting of two sections. Classical authors named them as 'sperm pump' and 'ejaculatory duct', based on superficial resemblance to a spring-loaded, thimble-shaped cylinder, encircled by smooth, vertical columns interpreted to be muscles which, when contracted, compress the cylinder and affect seminal fluid discharge. The discovery of numerous spherules of unknown composition and function in and around the columns of the Asian citrus psyllid male genitalia invoked rigorous scrutiny of the classical literature for evidence to support its claims, and determined that the grounds for vetting the structure as a sperm pump were fully teleological. This paper raises several objections to modern acceptance of this classical interpretation, presenting them as problematic, thought-provoking, and sometimes controversial anatomical features. The two sections are herein called 'drum' and 'spout'. As an endoskeleton, the sections are an invagination of the exoskeleton and therefore cannot receive seminal fluid into their hollow. A phallus is identified inside an aedeagal tube, indicating that it is the ejaculatory duct-the tube, drum, and spout are considered its housing. A sheath envelopes the drum and is directly continuous with the spout hypodermis, another problematical feature raising the question of whether it is detached from adherence to the drum cuticles. Also, there are four afferent tubes but only two openings in the drum to receive their seminal fluids.


Assuntos
Hemípteros/ultraestrutura , Animais , Genitália Masculina/fisiologia , Genitália Masculina/ultraestrutura , Hemípteros/fisiologia , Masculino , Terminologia como Assunto
3.
Acta Vet Scand ; 61(1): 25, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31146786

RESUMO

BACKGROUND: Although artificial insemination (AI) was developed as a means of controlling disease transmission, pathogens can still be transmitted to females in semen used for AI. In addition, bacteria can cause deterioration in sperm quality during storage. Semen becomes contaminated by the male's normal bacterial flora as it passes out of the reproductive tract but potential pathogens may also contaminate the semen. Therefore, semen samples from stallions to be used for AI are tested before the breeding season to minimize transmission of pathogens to inseminated mares. In Sweden, semen samples are tested at the National Veterinary Institute, Uppsala (SVA). For the present study, a retrospective analysis was made of potentially pathogenic bacteria isolated from samples submitted to the SVA from 2007 to 2017. RESULTS: In our study, Taylorella equigenitalis was found infrequently (53 out of 25,512 samples), representing 11 out of 2308 stallions. If T. equigenitalis was detected, the stallions were treated with antibiotics and re-tested later in the same year. Klebsiella pneumoniae and beta haemolytic streptococci were the most commonly found potential pathogens, whereas Pseudomonas aeruginosa was also isolated occasionally. There were considerable differences in the number of species isolated each year. CONCLUSIONS: Potential pathogens were identified in relatively few of the samples submitted to SVA during this period, with T. equigenitalis not being identified since 2015. Of the other potential pathogens, K. pneumoniae and beta haemolytic streptococci were the most common. The information is relevant for determining guidelines on the testing and treatment of stallions before breeding.


Assuntos
Infecções Bacterianas/veterinária , Fenômenos Fisiológicos Bacterianos , Líquidos Corporais/microbiologia , Genitália Masculina/microbiologia , Doenças dos Cavalos/microbiologia , Infecções do Sistema Genital/veterinária , Sêmen/microbiologia , Animais , Antibacterianos/uso terapêutico , Infecções Bacterianas/diagnóstico , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/microbiologia , Doenças dos Cavalos/diagnóstico , Doenças dos Cavalos/tratamento farmacológico , Cavalos , Inseminação Artificial , Masculino , Infecções do Sistema Genital/diagnóstico , Infecções do Sistema Genital/tratamento farmacológico , Infecções do Sistema Genital/microbiologia , Suécia
4.
Ann Agric Environ Med ; 26(2): 210-216, 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31232047

RESUMO

INTRODUCTION AND OBJECTIVE: In the light of current data concerning the growing exposure to ionizing radiation (IR) originating from atrificial sources, especially from medical ones, and also related to occupational exposure, it is justifiable to systematize the state of knowledge concerning the effect of IR on the male reproductive system. BRIEF DESCRIPTION OF THE STATE OF KNOWLEDGE: There is no basis for the application of the hypothesis of hormesis in the area of male reproductive health. Regarding the impact of IR on spermatogenesis, spermatogonia are less susceptible to the occurrence of DNA damage after exposition to IR, but are characterized by slower DNA repair compared to somatic cells. Damage to the genes after exposure to IR is possible at each stage of spermatogenesis; however, haploidal spermatids show the highest radiosensitivity in this respect. The genetic risk of the cells differentiating during spermatogenesis is limited to one cycle of spermatogenesis, whereas the genetic instability may persist for the whole period of life, and DNA damage induced by IR may be transmitted to future generations. The minimum dose causing detectable DNA damage was 30 Gy. While exceeding this dose, the number of single-strand DNA breaks increases. Among males exposed to IR, a decrease was observed in sperm motility and in the percentage of morphologically normal spermatozoa as well as in an intensification of vacuolization. The genetic material in the sperm of these males showed higher fragmentation and methylation of genomic DNA. CONCLUSION: In the context of the epidemiological situation concerning the prevalence of infertility, while assessing the health effects of exposure to IR from artificial, including medical sources, the reproductive risk should be considered.


Assuntos
Genitália Masculina/efeitos da radiação , Espermatozoides/efeitos da radiação , Animais , Dano ao DNA/efeitos da radiação , Humanos , Masculino , Radiação Ionizante , Espermatogênese/efeitos da radiação , Espermatozoides/citologia
5.
Parasite ; 26: 37, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31246168

RESUMO

Three new species of Synodontella Dossou & Euzet, 1993 are described from two species of Synodontis (Mochokidae) collected from the middle course of the Boumba River (East Cameroon): Synodontella angustupenis n. sp. from Synodontis nummifer, Synodontella longipenis n. sp. and Synodontella simplex n. sp. from Synodontis decorus. These new species are different from the other Synodontella species already described due to their horseshoe-shaped dorsal transverse bars. Synodontella angustupenis differs from S. longipenis and S. simplex by the morphology of its penis, a thin tube, and its accessory piece, bifid at its extremity. Synodontella longipenis differs from the other two species by the morphology and the size of its male copulatory organ, which is very long. Synodontella simplex differs from S. angustupenis and S. longipenis by the shape of its penis, which is simple, and of its accessory piece, with a developed heel. The difference between the dorsal transverse bars of Synodontella species from the Sanaga River in Cameroon (and other localities in Africa), slightly curved, and those from the Boumba River, horseshoe-shaped, makes it possible to separate Synodontella species into two different subgroups. This difference can be explained by a long isolation period of the hosts, living in different river basins, followed by the divergence of the parasite populations (vicariant speciation).


Assuntos
Peixes/parasitologia , Brânquias/parasitologia , Rios/parasitologia , Trematódeos/anatomia & histologia , Trematódeos/isolamento & purificação , Animais , Camarões , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Genitália Masculina/anatomia & histologia , Masculino , Trematódeos/classificação
6.
Gene ; 710: 218-232, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31158448

RESUMO

Alterations in the global gene expression profile are considered to contribute to the various physiological and pathological changes during the course of ageing. Genes that code for the molecular components of the innate system are alter markedly as ageing occurs; and this may define the susceptibility of very young and very old individuals to reproductive tract infections. The expression pattern of genes that code for beta-defensins (effectors of innate immune response) in male reproductive tract tissues of different stages of ageing is not yet reported. Further, the induction of beta-defensins during endotoxin challenge and whether epigenetic modulators can influence the expression of these genes in different stages of ageing are not reported. We analyzed the basal mRNA levels of beta-defensins and defensin-like proteins (Sperm Associated Antigen 11 (SPAG11) family members), their induction during endotoxin challenge and modulation by epigenetic modifiers (Trichostatin A and Azacytidine) in the caput, cauda, testis, prostate and seminal vesicle of rats that represent early stage to late stages of life (20 day to 730 day old). We observed differential basal gene expression pattern in the male reproductive tract tissues and the induction by LPS was not consistent neither among the age groups not the tissues analyzed. Trichostatin A and Azacytidine also influenced antimicrobial gene expression and the pattern was not consistent in different tissues obtained from different age groups. Results of this study demonstrate that antimicrobial gene expression varies to a great extent during ageing and is strongly influenced by endotoxins and epigenetic modulators.


Assuntos
Envelhecimento/genética , Genitália Masculina/química , Glicopeptídeos/genética , beta-Defensinas/genética , Animais , Azacitidina/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Genitália Masculina/efeitos dos fármacos , Ácidos Hidroxâmicos/farmacologia , Lipopolissacarídeos/farmacologia , Masculino , Ratos , Ratos Wistar
7.
Food Chem Toxicol ; 130: 231-241, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31121209

RESUMO

Furan is a colorless toxic organic compound that is produced during thermal degradation of natural food constituents, and is present in various processed foods such as coffee and processed baby foods. The present study investigated the endocrine disrupting potential of furan in Sprague Dawley male pups. On postnatal day 0 (PND 0), pups were divided into five groups. The control group received subcutaneous injections of corn oil (50 µL), while the treated groups were injected with one of four concentrations of furan (1, 5, 10 and 20 mg kg-1 d-1 in 50 µL corn oil) from PND 1 to PND 10. Our results reveal significant physiological changes in groups receiving the two highest doses of furan (10 and 20 mg kg-1 d-1). Fertility was decreased in high dose groups, as evidenced by lower daily sperm production (DSP) and epididymis sperm counts, and dose-dependent histological alterations in the testes. High dose groups showed significant reductions in plasma concentrations of testosterone, LH and GH, while plasma cortisol and final body weight was increased compared to the control group. .The results suggest that neonatal exposure to high concentrations of furan cause structural and endocrine alterations in male neonatal rats, compromising fertility.


Assuntos
Furanos/toxicidade , Genitália Masculina/efeitos dos fármacos , Genitália Masculina/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos , Relação Dose-Resposta a Droga , Disruptores Endócrinos/administração & dosagem , Disruptores Endócrinos/toxicidade , Furanos/administração & dosagem , Masculino , Ratos , Ratos Sprague-Dawley , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatogênese/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
8.
Fertil Steril ; 111(5): 895-896, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31029244

RESUMO

"All progress is precarious, and the solution of one problem brings us face to face with another problem." -Martin Luther King, Jr.


Assuntos
Infertilidade Masculina/diagnóstico , Infertilidade Masculina/terapia , Livros de Texto como Assunto , Genitália Masculina/fisiologia , Humanos , Masculino
9.
Int J Mol Sci ; 20(7)2019 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-30986927

RESUMO

In recent years, the mammalian target of rapamycin (mTOR) has emerged as a master integrator of upstream inputs, such as amino acids, growth factors and insulin availability, energy status and many others. The integration of these signals promotes a response through several downstream effectors that regulate protein synthesis, glucose metabolism and cytoskeleton organization, among others. All these biological processes are essential for male fertility, thus it is not surprising that novel molecular mechanisms controlled by mTOR in the male reproductive tract have been described. Indeed, since the first clinical evidence showed that men taking rapamycin were infertile, several studies have evidenced distinct roles for mTOR in spermatogenesis. However, there is a lack of consensus whether mTOR inhibition, which remains the experimental approach that originates the majority of available data, has a negative or positive impact on male reproductive health. Herein we discuss the latest findings concerning mTOR activity in testes, particularly its role on spermatogonial stem cell (SSC) maintenance and differentiation, as well as in the physiology of Sertoli cells (SCs), responsible for blood-testis barrier maintenance/restructuring and the nutritional support of spermatogenesis. Taken together, these recent advances highlight a crucial role for mTOR in determining the male reproductive potential.


Assuntos
Genitália Masculina/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Animais , Humanos , Masculino , Modelos Biológicos , Transdução de Sinais , Testículo/metabolismo
10.
Food Chem Toxicol ; 128: 193-201, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30986439

RESUMO

This study was aimed at determining whether dienestrol (DIES) affects reproduction in male offspring of rats following oral maternal exposure during gestation and lactation. Pregnant rats were treated from GD 6 to PND 21. Animals received 0 (control-vehicle), 0.75, 1.5, 3.12, 6.25, 12.5, 50, 75 µg/kg bw/d of DIES. A control group -without vehicle-was also included. High DIES concentrations caused abortions at 75 and 50 µg/kg bw/d, while at 12.5 µg/kg bw/d had still miscarriages. Ten male rats per group were kept alive until PND 90 to ensure sexual maturity. Body and organ weights, anogenital distance (AGD) at PNDs 21 and 90, biochemical and sperm parameters like motility, viability, morphology, spermatozoa and resistant spermatid counts, and histopathology for sexual organs and liver were determined. An increase in organ weight (liver and sexual organs) and a decrease in AGD due to vehicle were found. A reduction of sperm motility and viability, and an increase of abnormal sperm morphology were caused by DIES, which provoked a dose-dependent prostatitis. Maternal exposure to DIES induced toxicity on the reproductive system of the male offspring, which could affect the capacity of fertilization.


Assuntos
Dienestrol/toxicidade , Estrogênios não Esteroides/toxicidade , Genitália Masculina/efeitos dos fármacos , Exposição Materna , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Aborto Animal/induzido quimicamente , Administração Oral , Animais , Peso Corporal/efeitos dos fármacos , Dienestrol/administração & dosagem , Relação Dose-Resposta a Droga , Estrogênios não Esteroides/administração & dosagem , Feminino , Masculino , Tamanho do Órgão/efeitos dos fármacos , Gravidez , Prostatite/induzido quimicamente , Ratos , Contagem de Espermatozoides
11.
Nat Commun ; 10(1): 1312, 2019 03 21.
Artigo em Inglês | MEDLINE | ID: mdl-30899023

RESUMO

Male genitalia exhibit patterns of divergent evolution driven by sexual selection. In contrast, for many taxonomic groups, female genitalia are relatively uniform and their patterns of evolution remain largely unexplored. Here we quantify variation in the shape of female genitalia across onthophagine dung beetles, and use new comparative methods to contrast their rates of divergence with those of male genitalia. As expected, male genital shape has diverged more rapidly than a naturally selected trait, the foretibia. Remarkably, female genital shape has diverged nearly three times as fast as male genital shape. Our results dispel the notion that female genitalia do not show the same patterns of divergent evolution as male genitalia, and suggest that female genitalia are under sexual selection through their role in female choice.


Assuntos
Evolução Biológica , Besouros/anatomia & histologia , Genitália Feminina/anatomia & histologia , Genitália Masculina/anatomia & histologia , Animais , Besouros/classificação , Besouros/genética , Feminino , Genitália Feminina/fisiologia , Genitália Masculina/fisiologia , Masculino , Preferência de Acasalamento Animal/fisiologia , Fenótipo , Filogenia , Reprodução , Seleção Genética , Fatores Sexuais
12.
Environ Health ; 18(1): 15, 2019 03 12.
Artigo em Inglês | MEDLINE | ID: mdl-30857531

RESUMO

BACKGROUND: Glyphosate-based herbicides (GBHs) are broad-spectrum herbicides that act on the shikimate pathway in bacteria, fungi, and plants. The possible effects of GBHs on human health are the subject of an intense public debate for both its potential carcinogenic and non-carcinogenic effects, including potential effects on the endocrine system The present pilot study examine whether exposure to GBHs at the dose of glyphosate considered to be "safe" (the US Acceptable Daily Intake - ADI - of 1.75 mg/kg bw/day), starting from in utero life, affect the development and endocrine system across different life stages in Sprague Dawley (SD) rats. METHODS: Glyphosate alone and Roundup Bioflow, a commercial brand of GBHs, were administered in drinking water at 1.75 mg/kg bw/day to F0 dams starting from the gestational day (GD) 6 (in utero) up to postnatal day (PND) 120. After weaning, offspring were randomly distributed in two cohorts: 8 M + 8F/group animals belonging to the 6-week cohort were sacrificed after puberty at PND 73 ± 2; 10 M + 10F/group animals belonging to the 13-week cohort were sacrificed at adulthood at PND 125 ± 2. Effects of glyphosate or Roundup exposure were assessed on developmental landmarks and sexual characteristics of pups. RESULTS: In pups, anogenital distance (AGD) at PND 4 was statistically significantly increased both in Roundup-treated males and females and in glyphosate-treated males. Age at first estrous (FE) was significantly delayed in the Roundup-exposed group and serum testosterone concentration significantly increased in Roundup-treated female offspring from the 13-week cohort compared to control animals. A statistically significant increase in plasma TSH concentration was observed in glyphosate-treated males compared with control animals as well as a statistically significant decrease in DHT and increase in BDNF in Roundup-treated males. Hormonal status imbalances were more pronounced in Roundup-treated rats after prolonged exposure. CONCLUSIONS: The present pilot study demonstrate that GBHs exposure, from prenatal period to adulthood, induced endocrine effects and altered reproductive developmental parameters in male and female SD rats. In particular, it was associated with androgen-like effects, including a statistically significant increase of AGDs in both males and females, delay of FE and increased testosterone in female.


Assuntos
Glicina/análogos & derivados , Herbicidas/toxicidade , Canal Anal/anatomia & histologia , Canal Anal/efeitos dos fármacos , Animais , Sistema Endócrino/efeitos dos fármacos , Ciclo Estral/efeitos dos fármacos , Feminino , Genitália Feminina/anatomia & histologia , Genitália Feminina/efeitos dos fármacos , Genitália Masculina/anatomia & histologia , Genitália Masculina/efeitos dos fármacos , Glicina/toxicidade , Humanos , Masculino , Troca Materno-Fetal , Projetos Piloto , Gravidez , Ratos Sprague-Dawley , Maturidade Sexual/efeitos dos fármacos , Testosterona/sangue , Tireotropina/sangue , Testes de Toxicidade Subcrônica
13.
Environ Health ; 18(1): 26, 2019 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-30922319

RESUMO

BACKGROUND: Previous animal and ecological studies have provided evidence for an earlier sexual maturation in females in relation to fluoride exposure; however, no epidemiological studies have examined the association between fluoride exposure and pubertal development in both boys and girls using individual-level biomarkers of fluoride. Capitalizing on an ongoing Mexican birth cohort study, we examined the association between concurrent urinary fluoride levels and physical markers of pubertal development in children. METHODS: We conducted a cross-sectional study of 157 boys and 176 girls at age 10-17 years living in Mexico City. We used ion-selective electrode-based diffusion methods to assess fluoride levels in urine, adjusting for urinary specific gravity. Pubertal stages were evaluated by a trained physician. Associations of fluoride with pubertal stages and age at menarche were studied using ordinal regression and Cox proportional-hazard regression, respectively. RESULTS: In the entire sample, the geometric mean and interquartile range (IQR) of urinary fluoride (specific gravity adjusted) were 0.59 mg/L and 0.31 mg/L, respectively. In boys, our analysis showed that a one-IQR increase in urinary fluoride was associated with later pubic hair growth (OR = 0.71, 95% CI: 0.51-0.98, p = 0.03) and genital development (OR = 0.71, 95% CI: 0.53-0.95, p = 0.02). No significant associations were found in girls, although the direction was negative. CONCLUSIONS: Childhood fluoride exposure, at the levels observed in our study, was associated with later pubertal development among Mexican boys at age 10-17 years. Further research is needed to confirm these findings.


Assuntos
Exposição Ambiental , Fluoretos/urina , Puberdade , Adolescente , Criança , Cidades/epidemiologia , Estudos de Coortes , Feminino , Genitália Masculina/crescimento & desenvolvimento , Humanos , Masculino , México/epidemiologia , Razão de Chances
16.
Arthropod Struct Dev ; 50: 15-23, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30890365

RESUMO

Males of Panorpodidae possess a special sperm pump, through which they directly transfer seminal fluid to the female spermatheca. However, the sperm pump has not been studied in Panorpodes to date. Here, the structure of the sperm pump and the internal coupling of genitalia were investigated in the short-faced scorpionfly Panorpodes kuandianensis Zhong, Zhang, and Hua, 2011 using light and scanning electron microscopy. The sperm pump mainly consists of a piston, a pumping chamber, the anterior region of the aedeagal complex, the posterior region of the ejaculatory sac, and associated muscles. The piston as a propulsion apparatus is controlled by levator and depressor muscles. Its posterior region connects dorsally to the aedeagus via a joint. The pumping chamber is located between the piston and the aedeagus. The dorsal and ventral parameres were attached by retractor muscles. During copulation, the male phallotreme connects to the female copulatory pore to transfer sperm. Male gonostyli and parameres grasp the female to restrict the genitalia movement and impede her medigynium from retreating. The sperm ejaculatory mechanism of Panorpodes and the evolution of sperm transfer mode in insects are briefly discussed based on the structure of the sperm pump and the internal coupling of genitalia.


Assuntos
Insetos/fisiologia , Insetos/ultraestrutura , Animais , Feminino , Genitália Feminina/ultraestrutura , Genitália Masculina/ultraestrutura , Masculino , Microscopia Eletrônica de Varredura , Espermatozoides/fisiologia
17.
Gen Comp Endocrinol ; 275: 6-14, 2019 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-30710553

RESUMO

Sexual differentiation and primary and secondary sexual characteristics in male crustaceans are modulated by hormones produced in the androgenic gland (AG). The AG is also responsible for the determination of morphotypes in caridean shrimps, such as Macrobrachium amazonicum that shows four morphotypes: translucent claw (TC), cinnamon claw (CC), green claw 1 (GC1) and green claw 2 (GC2). Here, we verified the anatomical, histological and ultrastructural characteristics of the AG in different morphotypes of this species with both amphidromous and hololimnetic life cycles. In submissive morphotypes (TC and CC), the AGs are reduced and concentrated in the terminal expansion of the distal portion of vasa deferentia (DVD), the ejaculatory ducts (ED). In dominant morphotypes (GC1 and GC2) these glands lie along the DVD and ED. Two morphological stages (I and II) were recorded for AG cells. In submissive morphotypes stage I cells predominated in the AGs, while in dominant morphotypes stage II cells were more common. AG cells in both stages were positive for proteins, confirming the protein nature of the secreted hormone. Stage I cells have abundant rough endoplasmic reticulum (RER) with numerous parallel cisternae, whereas in stage II cells, the cisternae of RER are highly dilated. Stage II cells do not produce secretory granules, but they undergo hypertrophy and the hormone release to hemolymph probably occurs by holocrine secretion. The AGs in TC, GC1 and GC2 morphotypes increase as the animals grow and are larger in GC1 males. On the other hand, AGs decrease in the CC morphotype as the animal grows. These differences are related to the type of reproductive strategy adopted by each morphotype. In M. amazonicum, the AGs show the same morphological, histochemical and ultrastructural patterns between the different life history populations.


Assuntos
Genitália Masculina/anatomia & histologia , Palaemonidae/anatomia & histologia , Diferenciação Sexual/fisiologia , Androgênios/metabolismo , Animais , Brasil , Genitália Masculina/metabolismo , Estágios do Ciclo de Vida/fisiologia , Masculino , Palaemonidae/fisiologia , Fenótipo , Reprodução/fisiologia , Rios
18.
Virology ; 530: 19-26, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30763872

RESUMO

Zika virus (ZIKV) infection can result in neurological disorders including Congenital Zika Syndrome in infants exposed to the virus in utero. Pregnant women can be infected by mosquito bite as well as by sexual transmission from infected men. Herein, the variants of ZIKV within the male reproductive tract and ejaculates were assessed in inoculated mice. We identified two non-synonymous variants at positions E-V330L and NS1-W98G. These variants were also present in the passage three PRVABC59 isolate and infectious clone relative to the patient serum PRVABC59 sequence. In subsequent studies, ZIKV E-330L was less pathogenic in mice than ZIKV E-330V as evident by increased average survival times. In Vero cells, ZIKV E-330L/NS1-98G outcompeted ZIKV E-330V/NS1-98W within 3 passages. These results suggest that the E-330L/NS1-98G variants are attenuating in mice and were enriched during cell culture passaging. Cell culture propagation of ZIKV could significantly affect animal model development and vaccine efficacy studies.


Assuntos
Mutação , Infecção por Zika virus/patologia , Infecção por Zika virus/virologia , Zika virus/genética , Zika virus/patogenicidade , Animais , Cercopithecus aethiops , Modelos Animais de Doenças , Genitália Masculina/virologia , Humanos , Masculino , Camundongos , Espermatozoides/virologia , Células Vero , Zika virus/isolamento & purificação
19.
Zootaxa ; 4555(3): 416-424, 2019 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-30790927

RESUMO

The genus Gnathospinosa Liao Huang, gen. nov., belonging to the subfamily Euplocaminae, is described with a new species G. qinlingensis Liao Huang, sp. nov. from China as its type species. The new species was illustrated with photographs of the adults and male genitalia. A preliminary phylogenetic study based on mitochondrial cytochrome c oxidase subunit I gene (co1) sequence data of the new species and Psecadioides cuneus are presented.


Assuntos
Lepidópteros , Animais , China , Genes Mitocondriais , Genitália Masculina , Masculino , Filogenia
20.
Zootaxa ; 4559(3): 473-500, 2019 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-30791003

RESUMO

The taxonomy of the leafhopper genus Makilingia Baker is reviewed based on comparative morphological study of types and other specimens. Twenty-six species are recognized as valid including ten new species described and illustrated herein: M. davaoensis n. sp. M. lobata n. sp., M. maculamima n. sp., M. nigramima n. sp., M. paranigra n. sp., M. siamensis n. sp., M. tenebrifrons n. sp., M. uncinata n. sp., M. viraktamathi n. sp., and M. xanthopicta n. sp. Makilingia siamensis n. sp. represents the first known occurrence of the genus outside the Philippine Archipelago and the first record for Thailand. Makilingia simillima Baker, n. stat., formerly treated as a variety of M. variabilis Baker, is elevated to full species status based on distinctive differences in the male genitalia. Lectotypes are designated for several species described by Baker. The male genitalia of these species are described and illustrated for the first time and a key to all known species is provided.


Assuntos
Hemípteros , Animais , Genitália Masculina , Masculino , Tailândia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA