Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 68
Filtrar
Mais filtros










Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
Carbohydr Polym ; 223: 115127, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31426982

RESUMO

Pods from a Gleditsia sinensis Lam. tree were collected and the galactomannan content and other properties were determined at their different developmental stages. In green and immature seed, galactomannan was substituted to a great extent with a mannose to galactose (M/G) ratio of 2.4 from crude polysaccharides. During late galactomannan deposition, it was substituted to a lower extent and this ratio increased rapidly, reaching a M/G ratio of 3.1. Average molecular weight (Mw) of the extracted polysaccharides first increased, reached the maximum (1.19 × 106) at 17 weeks after flowering (WAF), and then decreased. These changes might result from primary galactomannan biosynthesis and from galactose removal by α-galactosidase in the endosperm. The solubility of crude polysaccharides decreased with increased M/G ratio and maximum solubility was more than 89% that collected at 13 WAF. Rheological properties showed that apparent viscosity was largely influenced by the molecular weight and M/G ratio of galactomannans.


Assuntos
Gleditsia/química , Mananas/química , Galactose/química , Galactose/metabolismo , Gleditsia/crescimento & desenvolvimento , Gleditsia/metabolismo , Mananas/biossíntese , Peso Molecular , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Reologia , Sementes/química , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Solubilidade
2.
Oncol Res ; 27(8): 889-899, 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-30940289

RESUMO

The thorns of Gleditsia sinensis have been historically used in Chinese medicine and are considered one of the fundamental therapeutic herbs. Its anticancer effects are currently being explored. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and still requires the development of new drugs with higher efficiency. By using a rat HCC model implanted with cancerous Walker-256 cells, the therapeutic effects of G. sinensis extract (GSE) were assessed, as well as its regulatory effects on miRNAs. GSE significantly restored liver morphology and dramatically induced cell apoptosis in HCC rats. In addition, miR-21/181b/183 was upregulated in the HCC liver, and the elevation of these miRNAs could be alleviated by both GSE and sorafenib. PTEN/TIMP3/PDCD4 downregulation was consistent with the targets of miR-21/181b/183 in the HCC liver, and the alteration of these target genes was restored by both GSE and sorafenib. TIMP3 effects on MMP-2/9 expression were also determined. Our present findings indicate the potential of GSE in HCC treatment, and expand the understanding of miRNA-related mechanisms in the anticancer effects of GSE.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Carcinoma Hepatocelular/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Mamárias Animais/tratamento farmacológico , Animais , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose/genética , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patologia , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Gleditsia/química , Humanos , Neoplasias Hepáticas/patologia , Neoplasias Mamárias Animais/genética , Neoplasias Mamárias Animais/patologia , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 9 da Matriz/genética , MicroRNAs/efeitos dos fármacos , PTEN Fosfo-Hidrolase/genética , Extratos Vegetais/farmacologia , Ratos , Inibidor Tecidual de Metaloproteinase-3/genética
3.
Nat Prod Res ; 33(2): 239-243, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29473435

RESUMO

A new neolignan, 5-(3″-acetoxypropyl)-2-(4'-hydroxy-3'-methoxyphenyl)-7-methoxy-3-methylbenzofuran (1) along with nine analogues were isolated from the thorns of Gleditsia japonica var. delavayi by solvent extraction and repeated column chromatography. Their structures were elucidated by means of extensive spectroscopic analysis including 1D, 2D-NMR techniques and HR-ESIMS. All the isolates were reported for the first time from this species.


Assuntos
Gleditsia/química , Lignanas/isolamento & purificação , Lignanas/química , Estrutura Molecular , Estruturas Vegetais/química , Análise Espectral
4.
Nat Prod Res ; 33(16): 2308-2313, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29473437

RESUMO

One new triterpenoid saponin (1), as well as six known ones (2-7), were isolated from the ethanol extract of the thorns of Gleditsia sinensis. Their structures were elucidated by extensive spectroscopic analysis in conjunction with chemical evidence. Cytotoxic activity of compounds 1-6 was evaluated against human breast cancer MCF 7 cells in vitro by the MTT method. Our results revealed moderate activities for compounds 1-6 with IC50 values of 18.43, 30.47, 18.46, 10.02, 30.76, and 17.32 µM, respectively. Furthermore, compounds 1, 3, 4, and 6 induced apoptosis in MCF 7 cell, with 1 and 6 causing late apoptosis of MCF 7 cells, while 3 and 4 acting oppositely.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Neoplasias da Mama/tratamento farmacológico , Gleditsia/química , Extratos Vegetais/análise , Saponinas/isolamento & purificação , Triterpenos/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Neoplasias da Mama/patologia , Feminino , Humanos , Células MCF-7 , Saponinas/química , Saponinas/farmacologia , Triterpenos/química , Triterpenos/farmacologia
5.
J Nat Med ; 73(2): 369-380, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30547286

RESUMO

Gleditsia sinensis is widely used as a medicinal plant in Asia, especially in China. Triterpenes, alkaloids, and sterols were isolated from Gleditsia species. Among them, triterpenoid saponins are very important metabolites owing to their various pharmacological activities. However, the triterpenoid saponin biosynthesis pathway has not been well characterized. In the present study, we performed de novo transcriptome assembly for 14.3 Gbps of clean reads sequenced from nine tissues of G. sinensis. The results showed that 81,511 unique transcripts (unitranscripts) (47,855 unigenes) were constructed, of which 31,717 unigenes were annotated with Gene Ontology and EC numbers by Blast2GO against the NCBI-nr protein database. We also analyzed the metabolite contents in the same nine tissues by LS-MS/MS, and saponins including gleditsioside I were found in fruit at higher levels. Many of the genes with tissue-specific expression in fruit are involved in the flavonoid biosynthesis pathway, and many of those have UDP-glucosyltransferase (UGT) activity. We constructed a saponin biosynthesis pathway and identified two key enzyme families in the triterpenoid saponin biosynthesis pathway, cytochrome P450 and UDP-glucosyltransferase, that are encoded by 37 unigenes and 77 unigenes, respectively. CYP72A, CYP716A, and CYP88D, which are known as key enzymes for saponin biosynthesis, were also identified among the P450s. Our results provide insight into the secondary metabolite biosynthesis and serve as important resources for future research and cultivation of G. sinensis.


Assuntos
Gleditsia/genética , Saponinas/biossíntese , Transcriptoma , Triterpenos/metabolismo , China , Perfilação da Expressão Gênica , Ontologia Genética , Gleditsia/química , Gleditsia/metabolismo , Metaboloma , Plantas Medicinais/química , Plantas Medicinais/genética , Saponinas/análise , Saponinas/genética , Análise de Sequência de RNA , Espectrometria de Massas em Tandem
6.
Carbohydr Polym ; 200: 122-127, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30177148

RESUMO

Waste paper has considerable potential as a raw material for lactic acid (LA) production due to high cellulose content, abundance and low cost. In this study, four kinds of waste papers were used for LA production through simultaneous saccharification and fermentation (SSF) by Streptococcus thermophilus. The SSF of office paper achieved the highest LA concentration (39.71 g/L), while the highest LA yield was observed for magazine (99.56%), followed by office paper (82.85%). High LA concentration is unfavorable to total LA conversion because of product inhibition. However, the addition of Gleditsia saponin (GS) could obtain both high yield and high concentration of LA at a low enzyme loading, indicating that product inhibition could be moderated. A lactic acid yield of 86.30% was obtained from office paper at an enzyme loading of 9 FPU/g-cellulose with GS, which was higher than that of without GS at a higher loading of 18 FPU/g-cellulose.


Assuntos
Fermentação , Gleditsia/química , Ácido Láctico/biossíntese , Papel , Saponinas/metabolismo , Streptococcus thermophilus/metabolismo , Resíduos , Biocatálise , Celulase/metabolismo , Celulose/metabolismo , Hidrólise , Lipase/metabolismo , Tensão Superficial
7.
Zhongguo Zhong Yao Za Zhi ; 43(16): 3249-3254, 2018 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-30200726

RESUMO

To study morphological characteristics change of the development process and quercetin and polyphenols of accumulation dynamic of the spines of Gleditsia sinensis, measure and compare morphological indexes using Vernier caliper, ruler and balance, calibrate and analysis quercetin and polyphenols content using HPLC and colorimetric method. The spines of G. sinensis development process is divided into formation period (the beginning of August to the beginning of November), dormant period (the beginning of November to the end of March in the following year), germination period (the end of March to the middle of April), fast growth period (the middle of April to the middle of August), browning period (the middle of August to the end of August) and mature period (the beginning of September to the end of December). Formation period the spines primordium divides and forms the scale bubs; dormant period the scale bubs are in a dormant state; germination period the bubs scales fall off, spines primordium began to development; fast growth period rapid growth to maximum; browning period browning from the tip and browns until the whole becomes brown; mature period The early stage of maturity is full of luster, gradually the color deepened and the luster faded. The accumulation of quercetin was gradually decreasing after increasing. The total polyphenol accumulation was significantly decreased and then gradually increased, decreased finally. The content of quercetin was increased from 0.000 4%-0.002 6%, and the polyphenol content decreased from 0.761 9%-0.049 1% and then slowly increased to 0.286 9% in the fast growth period.The quercetin continuous increase to 0.004 3% and total polyphenol increased to 0.421 6% in the browning period. In the mature period, the quercetin content significantly decreased after reaching 0.009 6% in September, and the polyphenols content decreased after reaching 0.723 5% in October. Using principal component analysis results: September first, October 2nd, November 3rd. The morphological characteristics change of the development process and quercetin and polyphenols accumulation were determined. The development process is divided into six periods, the best harvest time is the early stage of mature period. Provide theoretical support for the utilization of the spines of G. sinensis and cultivation techniques of high yield.


Assuntos
Gleditsia/química , Polifenóis/análise , Quercetina/análise , Gleditsia/crescimento & desenvolvimento , Compostos Fitoquímicos/análise , Plantas Medicinais/química , Estações do Ano
8.
J Pharm Biomed Anal ; 160: 64-72, 2018 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-30064041

RESUMO

The Gleditsia sinensis Lam thorn (GST) is a classical traditional Chinese medical herb, which is of high medical and economic value. GST could be easily adulterated with branch of Rosa multiflora thunb (BRM) and Rosa rugosa thumb (BRR), because of their similar appearances and much lower cost for these adulterants. In this study Fourier transform near-infrared spectroscopy (FT-NIR) combined with chemical pattern recognition techniques was explored for the first time to discriminate and quantify of cheaper materials (BRM and BRR) in GST. The Savitzkye-Golay (SG) smoothing, vector normalization (VN), min max normalization (MMN), first derivative (1 st D) and second derivative (2nd D) methods were used to pre-process the raw FT-NIR spectra. Successive projections algorithm was adopted to select the characteristic variables and linear discriminate analysis (LDA), support vector machine (SVM), as while as back propagation neural network (BPNN) algorithms were applied to construct the identification models. Results showed that BPNN models performance best compared with LDA and SVM models for it could reach 100% accuracy for identifying authentic GST, and GST adulterated with BRM and BRR based on the spectral region of 6500-5500 cm-1 combined with 1 st D pre-processing. In addition, the BRM and BRR content in adulterated GST were determined by partial least squares (PLS) regression. The correlation coefficient of prediction (rp), root mean square error of prediction (RMSEP) and bias for the prediction by PLS regression model were 0.9972, 1.969% and 0.3198 for BRM, 0.9972, 1.879% and 0.05408 for BRR, respectively. These results suggest that the combination of NIR spectroscopy and chemometric methods offers a simple, fast and reliable method for classification and quantification in the quality control of the tradition Chinese medicine herb of GST.


Assuntos
Contaminação de Medicamentos/prevenção & controle , Gleditsia/química , Modelos Químicos , Controle de Qualidade , Tecnologia Farmacêutica/métodos , Pós/análise , Reprodutibilidade dos Testes , Espectroscopia de Luz Próxima ao Infravermelho , Máquina de Vetores de Suporte , Tecnologia Farmacêutica/normas
9.
Chem Biodivers ; 15(8): e1800154, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29907997

RESUMO

Three new sulfated isoguanine alkaloid glycosides, designated as saikachinoside A monosulfate (1), saikachinoside A disulfate (2), and locustoside B disulfate (3), have been isolated from the pupal case of the wild bruchid seed beetle Bruchidius dorsalis (Chrysomelidae, Bruchinae) infesting the seed of Gleditsia japonica Miq. (Fabaceae). Their structures were determined by spectroscopic methods and the inhibitory activity of 2 and 3 against acid phosphatase was evaluated.


Assuntos
Alcaloides/farmacologia , Besouros/química , Gleditsia/química , Glicosídeos/farmacologia , Purinas/farmacologia , Sementes/química , Fosfatase Ácida/antagonistas & inibidores , Fosfatase Ácida/metabolismo , Alcaloides/química , Alcaloides/isolamento & purificação , Animais , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Glicosídeos/química , Glicosídeos/isolamento & purificação , Conformação Molecular , Pupa/química , Purinas/química , Purinas/isolamento & purificação , Relação Estrutura-Atividade , Sulfatos/química , Triticum/enzimologia
10.
J Sep Sci ; 41(8): 1752-1763, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29316281

RESUMO

Gleditsiae Spina, the thorn of Gleditsia sinensis Lam., has been used as an anti-inflammatory, anti-tumor, and anti-bacterial traditional medicine for hundreds of years in China. This study used high-performance liquid chromatography and tandem mass spectrometry combined with chemometric methods to allow the fast and accurate identification and quantification of the flavonoids compounds in Gleditsiae Spina, and created reliable criteria for accurate identification of Gleditsiae Spina and its adulterants. This research provides good evidence for the classification and quality evaluation of Gleditsiae Spina. Firstly, eight flavonoids compounds were detected and identified on the basis of their mass spectra, fragment characteristics, and comparison with published data. Then the mass spectroscopic fragmentation pathways of these compounds were determined and, in addition rutin, isoquercitrin, and quercitrin were detected in Gleditsiae Spina for the first time. The quantification was performed on a triple quadrupole tandem mass spectrometer in multi-reaction monitoring mode, and the baseline separation of the eight bioactive flavonoids components was achieved within 13 min. Furthermore, the proposed method was successfully applied for simultaneous quantitative determination of the eight Gleditsiae Spina compounds and adulterants obtained from different sources in China. Then, we built a classification model which showed a high level of accuracy predicting 100% of the samples, correctly.


Assuntos
Medicamentos de Ervas Chinesas/análise , Flavonoides/análise , Gleditsia/química , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem
11.
Carbohydr Polym ; 184: 127-134, 2018 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-29352903

RESUMO

The water-soluble polysaccharides extracted from endosperm of Gleditsia japonica var. delavayi seeds (EGSP) were identified as galactomannan having the M/G ratio of 2.54-2.66 and a weight average molecular weight (Mw) of 1913 kDa. The molecular structure of EGSP was determined by periodate oxidation, Smith degradation, methylation, FTIR and NMR spectroscopy. The main chain is composed of ß-1,4-d-mannopyranose and the branches composed of single α-1-d-galactopyranose. We had also established a model to speculate the fine structure of galactomannan molecules and given preliminary results. The I2-KI test indicated that there were many branches on the EGSP backbone and no starch in EGSP. The CD spectra and Congo red test showed EGSP was random coil conformations in solution and could form a small quantity of helical conformation under alkaline conditions. The microstructure of morphology was observed by OM, SEM and AFM. The results showed that the fibers composed of multiple microfibers formed a network construction by entangling with each other.


Assuntos
Endosperma/química , Gleditsia/química , Mananas/química , Sementes/química , Estrutura Molecular
12.
Phytochemistry ; 145: 168-178, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29156366

RESUMO

Three previously undescribed oleanane-type triterpenoidal saponins named caspicaosides L-N were isolated from the fruits of Gleditsia caspica Desf. The aglycons of these saponins were echinocystic acid, erythrodiol and 12-oleanene-3,28,30-triol. Caspicaoside L is a bisdesmosidic saponin acylated with two monoterpenic acids. It has a disaccharide moiety made up of glucose and arabinose attached to C-3 and pentasaccharide moiety linked to C-28 made up of one glucose, 2 xyloses, one inner rhamnose and one terminal rhamnose which was acylated with two identical monoterpenic acids. Caspicaoside M is a monodesmosidic saponin with a trisaccharide moiety at C-3 made up of glucose, xylose and arabinose, while caspicaoside N has a disaccharide moiety at C-3 made up of glucose and arabinose. Their structures were determined by extensive 1D and 2D (DQF-COSY, HSQC, TOCSY, 1H-13C-HSQC-TOCSY, HMBC, ROESY, NOESY) NMR, HRESIMS analyses and chemical degradation. The cytotoxicity MTT-based assay showed that caspicaosides M, N and L, respectively, exhibited high cytotoxic activity with IC50 ≤ 10 µM (72 h) at least against one of the three used cancer cell lines, MCF 7, A2780 and HT 29; and were 2-34 folds selective against the normal fibroblasts (MRC 5). All compounds also induced apoptosis and caused G2/M arrest in MCF 7 cells (24 h); thus showing pro-apoptotic properties.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Frutas/química , Gleditsia/química , Saponinas/farmacologia , Triterpenos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cristalografia por Raios X , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Modelos Moleculares , Estrutura Molecular , Saponinas/química , Saponinas/isolamento & purificação , Relação Estrutura-Atividade , Triterpenos/química , Triterpenos/isolamento & purificação
13.
Phytochemistry ; 143: 145-150, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28813687

RESUMO

Three previously undescribed isoguanine glycosides with an N3-prenyl group, designated locustoside B, saikachinoside B, and saikachinoside C, have been isolated from the seed of Gleditsia japonica Miquel (Fabaceae) along with two known compounds, locustoside A and saikachinoside A. Their structures were determined from spectroscopic data and X-ray crystallographic analysis. The inhibitory activity against acid phosphatase was evaluated.


Assuntos
Gleditsia/química , Sementes/química , Fosfatase Ácida/antagonistas & inibidores , Alcaloides , Cristalografia por Raios X , Fabaceae/química , Glicosídeos/química , Japão , Conformação Molecular , Ressonância Magnética Nuclear Biomolecular
14.
Carbohydr Polym ; 173: 1-6, 2017 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-28732846

RESUMO

Model systems formed by sodium caseinate (NaCAS) and espina corona gum (ECG) were studied. There was no evidence of attractive interactions between NaCAS and ECG macromolecules. Aqueous mixtures of NaCAS and ECG phase-separate segregatively over a wide range of concentrations. According to the images obtained by confocal laser scanning microscopy, NaCAS particles form larger protein aggregates when ECG is present in the system. An increase in the hydrodynamic diameter of NaCAS particles, as a result of ECG addition, was also observed by light scattering in diluted systems. A depletion-flocculation phenomenon, in which ECG is excluded from NaCAS surface, is proposed to occur in the concentrated mixed systems, resulting in NaCAS aggregation. ECG raises the viscosity of NaCAS dispersions without affecting the Newtonian flow behaviour of NaCAS. These results contribute to improve the knowledge of a barely-studied hydrocolloid which may be useful in the development of innovative food systems.


Assuntos
Caseínas/química , Gleditsia/química , Mananas/química , Animais , Bovinos , Viscosidade
15.
Nat Prod Res ; 31(3): 275-280, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27690627

RESUMO

One new flavanocoumarin (1), as well as six known flavonoids (2-7), was isolated from the ethyl acetate extract of the thorns of Gleditsia sinensis. The structures of these compounds were elucidated by extensive spectroscopic measurements and comparison with data reported in literatures. Cytotoxic activities of compounds 1-6 were evaluated against human liver cancer SK-hep-1 cells in vitro by the MTT method, with compound 1 displaying moderate activity (IC50 of 62.53 µM). Furthermore, compound 1 could increase the number of apoptosis cells in a concentration-dependent manner.


Assuntos
Antineoplásicos/isolamento & purificação , Cumarínicos/farmacologia , Gleditsia/química , Antineoplásicos/química , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Cumarínicos/química , Cumarínicos/isolamento & purificação , Flavonoides/química , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Estrutura Molecular , Extratos Vegetais/química , Análise Espectral
16.
Molecules ; 21(12)2016 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-27999373

RESUMO

The seeds of Gleditsia sinensis and Gleditsia microphylla, widespread in China, are an important source of galactomannans. G. sinensis gum (GSG) and G. microphylla gum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol. The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance. The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively. To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol). The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent. A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.


Assuntos
Gleditsia/química , Mananas/química , Gomas Vegetais/química , Sementes/química , 2-Propanol/química , Cromatografia em Gel , Etanol/química , Galactose/análise , Manose/análise , Peso Molecular , Viscosidade
17.
J Ethnopharmacol ; 192: 192-200, 2016 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-27401288

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Zao-Jiao-Ci (ZJC), as the spine of Chinese Honey locust (Gleditsia sinensis Lam.), is traditionally used as Chinese medicine to reduce inflammation. AIM OF THE STUDY: The present study aimed to investigate an anti-inflammatory effect of ZJC aqueous extract both in vitro and in vivo, as well as its underlying mechanisms. MATERIALS AND METHODS: Anti-inflammatory effect of ZJC aqueous extract was evaluated by using carrageenan-induced paw edema in rats. In addition, the inhibitory effects of ZJC on nitric oxide production, intracellular reactive oxygen species production, pro-inflammatory mediator expression and prostaglandin E2 (PGE2) production were determined by using LPS-activated RAW 264.7 cells. The anti-oxidant activity of ZJC was assessed using 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay. RESULTS: ZJC aqueous extract showed significant suppressive effect on paw edema in rats at 100mg/kg. Moreover, ZJC aqueous extract decreased the expression of cyclooxygenase (COX)-2 and significantly decreased the PGE2, tumor necrosis factor-α, interleukin (IL)-1ß and IL-6 production in LPS-activated macrophages in dose-dependent manners. ZJC aqueous extract inhibited the mRNA expression of these inflammatory cytokines as well. Furthermore, ZJC aqueous extract was found as an anti-oxidant and could inhibit ROS production in the LPS-induced cells. CONCLUSIONS: These findings show the potential of ZJC aqueous extract as a naturally occurring COX-2 inhibitor to reduce inflammation.


Assuntos
Benzopiranos/farmacologia , Inibidores de Ciclo-Oxigenase 2/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Edema/prevenção & controle , Gleditsia/química , Macrófagos/efeitos dos fármacos , Caules de Planta/química , Animais , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Benzopiranos/isolamento & purificação , Carragenina , Ciclo-Oxigenase 2/metabolismo , Inibidores de Ciclo-Oxigenase 2/isolamento & purificação , Citocinas/genética , Citocinas/metabolismo , Dinoprostona/metabolismo , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Medicamentos de Ervas Chinesas/isolamento & purificação , Edema/induzido quimicamente , Edema/genética , Edema/metabolismo , Regulação da Expressão Gênica , Mediadores da Inflamação/metabolismo , Macrófagos/metabolismo , Masculino , Camundongos , Fitoterapia , Plantas Medicinais , Células RAW 264.7 , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Solventes/química , Água/química
18.
Z Naturforsch C J Biosci ; 71(7-8): 253-60, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27259229

RESUMO

The fruits of Gleditsia species (Fabaceae) have been known in traditional medicine as a saponin-rich herbal medicine. The present study aimed to investigate the effects of the total methanolic extract of Gleditsia caspica (MEGC) and its saponin-containing fractions (SFGC) on hyperglycemia in streptozotocin (STZ)-induced diabetic rats. A single intraperitoneal injection of STZ (55 mg/kg body weight) was used to induce hyperglycemia in male albino rats. MEGC (15, 30 and 60 mg/kg, p.o.) and SFGC (15, 30 and 60 mg/kg, p.o.) were administered to the diabetic rats daily for 14 days. The anti-diabetic drug gliclazide (10 mg/kg, p.o.) was used as a positive control. Blood samples were collected from overnight fasted rats for the evaluation of the antihyperglycemic, antihyperlipidemic and antioxidant activities. The levels of glucose, triglycerides (TG), cholesterol (TC) and malondialdehyde (MDA) were increased significantly, whereas the levels of α-amylase, insulin and reduced glutathione (GSH) were decreased in the experimental diabetic rats. Pancreas and liver of the diabetic rats exhibited significant changes in the histopathology, morphology and DNA content. Administration of MEGC or SFGC led to a decrease in the levels of glucose, TG, TC and MDA. In addition, the levels of α-amylase, insulin and GSH were increased in MEGC and SFGC treated diabetic rats. Also, the histopathological and morphological changes, as well the changes in DNA were significantly reversed by the extracts. Thus, MEGC and SFGC exhibited potent hypoglycemic and hypolipidemic activities in STZ- induced diabetic rats.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Frutas/química , Gleditsia/química , Hipoglicemiantes/farmacologia , Extratos Vegetais/farmacologia , Saponinas/farmacologia , Animais , Glicemia/análise , Colesterol/sangue , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/induzido quimicamente , Relação Dose-Resposta a Droga , Glutationa/sangue , Hipoglicemiantes/química , Insulina/sangue , Fígado/efeitos dos fármacos , Fígado/patologia , Masculino , Malondialdeído/sangue , Metanol/química , Pâncreas/efeitos dos fármacos , Pâncreas/patologia , Fitoterapia , Extratos Vegetais/química , Ratos Wistar , Estreptozocina , Resultado do Tratamento , Triglicerídeos/sangue , alfa-Amilases/sangue
19.
Planta Med ; 82(18): 1558-1567, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27352387

RESUMO

A comprehensive two-dimensional liquid chromatography coupled with mass spectrometry method was established to separate and characterize triterpenoid saponins in Gleditsia sinensis, the Chinese herbal medicine Zhu-Ya-Zao used for the treatment of apoplexy. The saponins were separated on an Agilent Zorbax Eclipse plus C18 column (2.1 mm × 150 mm, 1.8 µm) for the first dimension, and a Poroshell 120 phenyl-hexyl column (3.0 mm × 50 mm, 2.7 µm) for the second dimension. Methanol and acetonitrile were used as the organic mobile phase for 1D and 2D, respectively. The theoretical peak capacity was 640, and the orthogonality was 57 %. Particularly, saponins with different numbers of monoterpene groups could be well separated on the second dimension. The structures were characterized by electrospray ionization tandem mass spectrometry in both positive and negative ion modes. In the MS/MS spectra, the relative abundances for [B α + Na]+ and [Y 0α + Na]+ were closely correlated with the number of sugar residues of the α-chain and ß-chain, respectively, which facilitated the differentiation of isomers. Finally, a total of 72 saponins with molecular weights greater than 1500 Da were characterized. Among them, 49 compounds, including 2 acetylated saponins, were detected from G. sinensis for the first time.


Assuntos
Gleditsia/química , Saponinas/química , Triterpenos/química , Fracionamento Químico , Extratos Vegetais/química , Saponinas/isolamento & purificação , Espectrometria de Massas em Tandem , Triterpenos/isolamento & purificação
20.
Int J Mol Sci ; 17(3): 328, 2016 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-26950116

RESUMO

Gleditsia sinensis thorns (GST) have been used as a traditional medicine for carbuncles and skin diseases. The purpose of this study was to decide whether non-toxicological levels of water extract of GST (WEGST) are effective in inhibiting the progress of prostate cancer formation and to identify the target molecule involved in the WEGST-mediated inhibitory process of prostate cancer cell migration and in vivo tumor formation. Through the Boyden chamber migration assay, we found that non-toxic levels of WEGST could not attenuate the PC3 migration to the bottom area coated with serum but significantly inhibited PC3 cell migration to the collagen-coated bottom area. We also found that non-toxic levels of WEGST significantly attenuated collagen against adhesion. Interestingly, ectopic administration of WEGST could not affect the expression of α2ß1 integrin, which is known as a receptor of collagen. However, when the PC3 cells adhered to a collagen-coated plate, the expression of α2 integrin but not that of ß1 integrin was significantly inhibited by the administration of non-toxic levels of WEGST, leading to the inhibition of focal adhesion kinase (FAK) phosphorylation. Furthermore, oral administration of WEGST (25 mg/kg/day) significantly inhibited the size of a PC3 cell-xenografted tumor. Taken together, these results suggest a novel molecular mechanism for WEGST to inhibit prostate cancer progression at particular stages, such as collagen-mediated adhesion and migration, and it might provide further development for the therapeutic use of WEGST in the treatment of prostate cancer progression.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Movimento Celular/efeitos dos fármacos , Gleditsia/química , Integrina alfa2beta1/metabolismo , Extratos Vegetais/uso terapêutico , Próstata/efeitos dos fármacos , Neoplasias da Próstata/tratamento farmacológico , Animais , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Colágeno/metabolismo , Proteína-Tirosina Quinases de Adesão Focal/metabolismo , Humanos , Masculino , Camundongos Nus , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Próstata/metabolismo , Próstata/patologia , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Transdução de Sinais/efeitos dos fármacos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA