Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.044
Filtrar
1.
Zhongguo Zhong Yao Za Zhi ; 45(3): 579-583, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237516

RESUMO

This study is to investigate the chemical constituents from the whole plant Corydalis edulis. The chemical constituents were separated and purified by macroporous resin D101, silica gel, Sephadex LH-20, ODS, and semi-preparative HPLC. Their structures were determined by physicochemical properties and spectroscopic data. Four compounds were isolated from the dichloromethane and water extracts of the whole plant C. edulis, and identified as 6'-ß-D-xylosylicariside B2(1),(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one(2), loliolide(3), and 5,5'-dimethoxybiphenyl-2,2'-diol(4), respectively. Compound 1 is a new compound, of which the absolute configuration was established by electronic circular dichroism(ECD) calculations. Compound 4 is obtained from the plants of Papaveraceae family for the first time. Compounds 2 and 3 are firstly isolated from the Corydalis genus.


Assuntos
Corydalis/química , Glicosídeos/isolamento & purificação , Sesquiterpenos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação
2.
Zhongguo Zhong Yao Za Zhi ; 45(5): 1114-1119, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32237454

RESUMO

Polygonflavanol B(1), a new flavonostilbene glycoside, was isolated from the roots of Polygonum multiforum(Polygonaceae) by various column chromatography methods including macroporous resin HP-20, silica gel, Sephadex LH-20, and preparative HPLC. The structure with absolute configuration of the new compound was identified by its physicochemical properties, spectroscopic data, ECD calculation, and chemical method.


Assuntos
Fallopia multiflora/química , Flavonóis/química , Glicosídeos/química , Raízes de Plantas/química , Estilbenos/química , Flavonóis/isolamento & purificação , Glicosídeos/isolamento & purificação , Estilbenos/isolamento & purificação
3.
Chem Pharm Bull (Tokyo) ; 68(3): 273-287, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32115535

RESUMO

Phytochemical analysis of the whole Helleborus foetidus plants identified 28 steroidal glycosides (1-28), including 20 novel spirostanol glycosides (1-20) and a novel furostanol glycoside (21). The structures of the newly identified compounds were elucidated by two-dimensional NMR spectroscopy and hydrolytic cleavage. Compounds 12, 13, and 15 were determined to be spirostanol trisdesmosides bearing sugar moieties at the C-1, -21, and -24 hydroxy groups of the aglycone unit. The isolated compounds were subsequently evaluated for cytotoxic activity against HL-60 human promyelocytic leukemia cells and A549 human lung carcinoma cells. In particular, 7 showed cytotoxic activity against the HL-60 and A549 cells, with IC50 values of 5.9 and 6.6 µM, respectively, whereas 19 was selectively cytotoxic to A549 cells with an IC50 value of 5.5 µM.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Glicosídeos/farmacologia , Helleborus/química , Compostos Fitoquímicos/farmacologia , Esteroides/farmacologia , Células A549 , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/química , Glicosídeos/isolamento & purificação , Células HL-60 , Humanos , Conformação Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Esteroides/química , Esteroides/isolamento & purificação , Relação Estrutura-Atividade
4.
Z Naturforsch C J Biosci ; 75(1-2): 51-56, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-32053496

RESUMO

The leaves of Garcinia epunctata Stapf have furnished two new phenolic glucosides, epunctosides A and B, along with 13 known secondary metabolites identified as lanceoloside A, betulinic acid, lupeol, stigmasterol, ß-sitosterol, ß-sitosterol-3-O-ß-d-glucopyranoside, stigmasterol-3-O-ß-d-glucopyranoside, luteolin-7-O-glucoside, quercitin-7-O-glucoside, amentoflavone, robustaflavone, 4'-O-methyl-amentoflavone and 4'-O-methyl-robustaflavone. All structures were established from chemical and spectroscopic evidence including 1D and 2D NMR data as well as by comparing the obtained spectroscopic data with literature. This is the first report of the presence of phenolic glucosides in the genus Garcinia.


Assuntos
Garcinia/química , Glicosídeos/química , Fenóis/química , Folhas de Planta/química , Biflavonoides/química , Glicosídeos/isolamento & purificação , Triterpenos Pentacíclicos/química , Fenóis/isolamento & purificação , Metabolismo Secundário/genética , Sitosteroides/química , Estigmasterol/química , Triterpenos/química
5.
Phytochemistry ; 171: 112232, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31911266

RESUMO

Corni Fructus, also known as the fruit of Cornus officinalis Sieb. et Zucc., has long been used as a traditional Chinese medicine and is widely consumed as a nutritional food in the form of function drink and wine. Recently, Corni Fructus has attracted considerable interest because of its anti-diabetic effects. A systematic phytochemical investigation of Corni Fructus was performed to find anti-diabetic components, which led to the isolation of 10 unreported iridoid glycosides, cornusdiglycosides A-J (1-8, 9a/9b and 10a/10b). Their chemical structures were determined through spectroscopic analysis (ultraviolet [UV], infrared [IR], high-resolution electrospray ionisation mass spectroscopy [HRESIMS], one-dimensional [1D] and two-dimensional [2D] nuclear magnetic resonance [NMR]). Such morroniside-type diglycosides were first reported from natural sources, and all isolates were evaluated for α-glucosidase inhibitory activity. The results showed that all compounds (1-10) exhibited α-glucosidase (from Saccharomyces cerevisiae) inhibitory activities with IC50 values ranging from 78.9 ± 4.09 to 162.2 ± 9.17 µM, whereas acarbose, the positive control, displayed α-glucosidase inhibitory activity with IC50 value of 118.9 ± 7.89 µM.


Assuntos
Cornus/química , Inibidores de Glicosídeo Hidrolases/farmacologia , Glicosídeos/farmacologia , Glucosídeos Iridoides/farmacologia , Compostos Fitoquímicos/farmacologia , alfa-Glucosidases/metabolismo , Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Glucosídeos Iridoides/química , Glucosídeos Iridoides/isolamento & purificação , Conformação Molecular , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação
6.
J Chromatogr A ; 1615: 460719, 2020 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-31767257

RESUMO

In general, the simultaneous separation and isolation of compounds with a broad polarity range from natural products is a challenge by ordinary high-speed counter-current chromatography (HSCCC). Indeed, the complex solvent system screening methods limit the broader application of HSCCC. We describe herein a rapid and efficient linear gradient CCC (LGCCC) method that enables the separation of flavonoid glycosides with a broad range of KD values from custard apple leaves. Inner-recycling CCC (IRCCC) mode has been further applied for the separation of compounds with similar KD values. Similarly to binary gradient HPLC, the LGCCC mode is achieved by adjustment of the proportion between ethyl acetate (pump A) and n-butanol (pump B) in an ethyl acetate/n-butanol/water solvent system. Various separation factors have been investigated, including separation mode, rotation speed, flow rate, and sample loading. The IRCCC mode has been used for the secondary separation of two epimers with a simple ethyl acetate/water (1:1, v/v) solvent system. Finally, five main flavonoid glycosides have been successfully separated, namely quercetin-3-O-robinobioside (1, 4.8 mg) and rutin (2, 12.1 mg), quercetin-3-O-ß-d-glucoside (3, 4.2 mg), kaempferol-3-O-robinobioside (4, 9.6 mg), and kaempferol-3-O-rutinoside (5, 24.6 mg). The purities of the separated flavonoid glycosides were over 98%, as determined by HPLC. Our study indicates that a suitable combination of LGCCC and IRCCC modes is an effective strategy for separating flavonoid glycosides from custard apple leaves. The mathematical expression of the LGCCC was deduced to illuminate the separation mechanism. It may also be applied to obtain component fractions for the further screening of active compounds from complex natural products.


Assuntos
Annona/química , Técnicas de Química Analítica/métodos , Distribuição Contracorrente , Glicosídeos/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Flavonoides/química , Flavonoides/isolamento & purificação , Quercetina/análise , Solventes/química
7.
Biomed Chromatogr ; 34(3): e4754, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31777094

RESUMO

A rapid, highly sensitive, and specific analytical method using ultra-high-performance liquid chromatography-triple quadruple MS was developed to quantitatively measure 18 chemical constituents in Huangqi Jianzhong Tang (HQJZ), a famous traditional Chinese medicine formula. Chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 µm) with a gradient mobile phase (A: 0.1% aqueous formic acid and B: acetonitrile) at a flow rate of 0.25 mL/min. The chromatographic peaks of 18 components were identified by comparing with the reference compounds. Multiple reaction monitoring was employed for quantitative analysis of the components. Seven batches of HQJZ samples were analyzed with a good linear regression relationship (R2 , .9978-.9993), precisions [relative standard deviation (RSD), 0.90%-3.60%], repeatability (RSD, 2.50%-4.00%), stability (RSD, 1.00%-4.00%), and recovery (96.10%-104.30%). Based on this established method, the present study offered a highly sensitive, specific, and rapid determination method for identification of 18 compounds, which greatly promoted the systemic quality control of HQJZ.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Flavonoides/análise , Glicosídeos/análise , Espectrometria de Massas em Tandem/métodos , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Modelos Lineares , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
8.
Phytochemistry ; 170: 112181, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31727321

RESUMO

Gymnema sylvestre (Retz.) R. Br. ex Schult. has a long history to be used as an antidiabetic herbal medicine. Various varieties of G. sylvestre, have been studied intensively on their 3ß-hydroxy oleanane triterpenoid composition for hypoglycemic effects. It is also well-known that most species belonging to the same genus have similar chemical composition and biological activity. Thus, an extract of the Gymnema latifolium Wall. ex Wight, which showed considerable protein tyrosine phosphatase 1B (PTP1B) inhibitory activity (>70% inhibition at 30 µg/mL), was studied intensively. Extensive chemical investigation on the 70% EtOH of G. latifolium led to the isolation of four previously undescribed oleanane hemiacetal glycosides, gymlatinosides GL1-GL4, three previously undescribed oleanane glycosides, gymlatinosides GL5-GL7, and two known 3ß-hydroxy oleanane analogs. The structures of the previously undescribed compounds were elucidated using diverse spectroscopic methods. The hemiacetal structure of the glycoside portion was further elaborated precisely by HMBC and J resolved proton NMR. Gymlatinosides GL2 and GL3 showed considerable PTP1B inhibitory effect.


Assuntos
Inibidores Enzimáticos/farmacologia , Glicosídeos/farmacologia , Gymnema/química , Ácido Oleanólico/análogos & derivados , Compostos Fitoquímicos/farmacologia , Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Estrutura Molecular , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Ácido Oleanólico/farmacologia , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação , Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo , Proteínas Recombinantes/metabolismo , Relação Estrutura-Atividade
9.
Nat Prod Res ; 34(7): 903-908, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30856347

RESUMO

A new allo-cedrane sesquiterpene glycoside (1) and nine known compounds (2-10) were isolated from the ethanol extract of the fruit of Illicium simonii Maxim. Their structures were elucidated by spectroscopic methods, including 1D-, 2D-NMR, and HRESIMS. The absolute configuration of compound 1 was confirmed by CD experiments. Among them, compounds 1, 4, 5, and 7 displayed moderate anti-inflammatory activities by use of an in vitro bioassay.


Assuntos
Frutas/química , Glicosídeos/isolamento & purificação , Illicium/química , Sesquiterpenos/isolamento & purificação , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Glicosídeos/química , Estrutura Molecular , Sesquiterpenos/química , Análise Espectral
10.
Nat Prod Res ; 34(3): 398-404, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30602316

RESUMO

Two new phenolic glycosides, named lanatusosides C (1) and D (2), together with four known compounds (3-6), were isolated from the seeds of Citrullus lanatus. Among them, compounds 3 and 4 were isolated from Cucurbitaceae for the first time, and compound 5 was reported from this plant for the first time. Their structures were elucidated by means of extensive spectral analysis, including HR-ESI-MS, 1H and 13C NMR techniques. The isolated new compounds were evaluated for cytotoxic activity against HepG2 cell line, of which compound 1 demonstrated weak cytotoxicity against the tested cell line.


Assuntos
Citrullus/química , Glicosídeos/isolamento & purificação , Sementes/química , Cucurbitaceae , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/química , Glicosídeos/toxicidade , Células Hep G2 , Humanos , Estrutura Molecular , Fenóis/química , Fenóis/isolamento & purificação , Fenóis/toxicidade
11.
Nat Prod Res ; 34(8): 1061-1067, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30663364

RESUMO

From the MeOH extract of the Vietnamese sea cucumber Holothuria edulis, eight triterpene glycosides (1-8), including one new compound namely holothurin A5 (1), were isolated by using various chromatographic separations. Their structures were established by spectroscopic experiments including 1D, 2D NMR and HR-ESI-MS. Holothurin A5 (1) has a hydroperoxy group at C-25. To the best of our knowledge, this is the first report of this group in triterpene saponins obtained from sea cucumbers to date. In addition, the in vitro cytotoxicity against five human cancer cell lines (HepG2, KB, LNCaP, MCF7 and SK-Mel2) of all isolated compounds was also evaluated using SRB assays.


Assuntos
Glicosídeos/isolamento & purificação , Holothuria/química , Pepinos-do-Mar/química , Triterpenos/isolamento & purificação , Animais , Linhagem Celular Tumoral , Citotoxinas/química , Citotoxinas/isolamento & purificação , Citotoxinas/farmacologia , Glicosídeos/química , Humanos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Saponinas/química , Saponinas/isolamento & purificação , Triterpenos/química , Vietnã
12.
Nat Prod Res ; 34(7): 975-980, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30600724

RESUMO

An ongoing chemical investigation on n-BuOH extract of roots and rhizomes of Helleborus thibetanus afforded three new spirostanol glycosides (1-3). Their structures were elucidated by extensive analysis of 1 D, 2 D NMR spectra, together with IR and MS methods and acid hydrolysis. This is the first report of the isolation of spirostanol glycoside with xylose at C-24 of the aglycone in Helleborus.


Assuntos
Glicosídeos/isolamento & purificação , Helleborus/química , Espirostanos/química , Glicosídeos/química , Hidrólise , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Raízes de Plantas/química , Rizoma/química , Saponinas/química
13.
Nat Prod Res ; 34(2): 233-240, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30636443

RESUMO

A new narciclasine glycoside, narciclasine-4-O-ß-D-xylopyranoside (1) was characterised along with four known alkaloids pancratistatin (2), 1-O-(3-hydroxybutyryl) pancratistatin (3), vittatine (4), 9-O-demethylgalanthine (5) from Zephyranthes minuta. Their structures were established on the basis of spectroscopic data analysis. The in vitro cytotoxic study of extract, fractions and isolated compounds against two human cancer cell lines (KB and SiHa) indicated the potential activity of extract and n-butanol fraction due to presence of active alkaloids pancratistatin, 1-O-(3-hydroxybutyryl) pancratistatin, lycorine and haemanthamine.


Assuntos
Alcaloides de Amaryllidaceae/isolamento & purificação , Amaryllidaceae/química , Glicosídeos/isolamento & purificação , Fenantridinas/isolamento & purificação , Alcaloides/química , Alcaloides/farmacologia , Alcaloides de Amaryllidaceae/química , Alcaloides de Amaryllidaceae/farmacologia , Glicosídeos Cardíacos , Linhagem Celular Tumoral , Citotoxinas/isolamento & purificação , Citotoxinas/farmacologia , Glicosídeos/química , Humanos , Isoquinolinas/farmacologia , Fenantridinas/química , Fenantridinas/farmacologia , Extratos Vegetais/química
14.
Nat Prod Res ; 34(2): 311-316, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30404547

RESUMO

Development of a novel agent for control of schistosomiasis is a mandate. In-vitro anti-schistosomal activity of the aerial parts of Huernia saudi-arabica were examined. Chromatographic investigations of the ethanol extract (EE) were afforded three compounds. Pregnane glycoside (CI) 12-ß-p-hydroxy-benzoyl-20-O-acetyl-boucerin-3-O-ß-D-glucopyranosyl-(1→4)-ß-D-cymaropyranosyl-(1→4)-ß-D-cymaropyranoside, in addition to two flavonoids (CII) luteolin-4'-O-ß-D-neohesperidoside and (CIII)quercetin-3-rutinoside were recognized via spectral analysis. The schistosomicidal effects were evaluated using scanning electron microscope (SEM). In-vitro bioassays on the viability (mobility, morphological changes and mortality) of Schistosoma mansoni adults, cercariae, miracidia and eggs at different concentrations 2.5, 5, 12.5, 25 and 50 µg/ml of EE and 2.6, 5.2, 13, 26 and 52 µM of CI in incubation times 1,2,4,6,12hrs were carried out. EE and CI evidenced in-vitro anti-schistosomal activity with a dose and incubation time-dependent fashion. The effect of EE and CI was evident by the topography damage showed by SEM. EE proved moderate in-vitro cytotoxicity with IC50 of 8.48 µg/ml.


Assuntos
Apocynaceae/química , Glicosídeos/farmacologia , Pregnanos/farmacologia , Esquistossomicidas/isolamento & purificação , Animais , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Glicosídeos/isolamento & purificação , Extratos Vegetais/química , Pregnanos/isolamento & purificação , Schistosoma mansoni/efeitos dos fármacos , Esquistossomicidas/farmacologia
15.
Nat Prod Res ; 34(6): 816-822, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30398365

RESUMO

The chemical constituents of Cupressus macrocarpa were investigated. A new neolignan glycoside (1) in addition to nine known compounds were isolated. The acetylcholinesterase (AChE) inhibitory activity and antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) of different fractions and isolates of C. macrocarpa were evaluated. The light petroleum fraction showed the highest activity in both assays with IC50 value of 88.79 µg/ml and 152.58 µg/ml for the AChE inhibitory activity and MRSA antibacterial activities, respectively. Weak to moderate activity were detected for the isolated compounds.


Assuntos
Antibacterianos/isolamento & purificação , Inibidores da Colinesterase/isolamento & purificação , Cupressus/química , Antibacterianos/farmacologia , Inibidores da Colinesterase/farmacologia , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Lignanas/isolamento & purificação , Lignanas/farmacologia , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Extratos Vegetais/química , Folhas de Planta/química
16.
Nat Prod Res ; 34(6): 773-781, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30445848

RESUMO

New allelochemicals were identified through bio-guided fractionation from the ethyl acetate of seeds extracts, which was the most autotoxic compared to the other plant parts. Phytochemical investigation of the seeds extracts of C. arabica by spectroscopy analyses has led to identify two new dammarane type triterpenes (4 and 9), with nine known analogues (1 - 3, 5 - 8, 10 and 11), a new cucurbitane triterpene (12), acylated dehydrodiconiferyl alcohol (13), and three highly oxygenated flavonols (14-16). The most autotoxic compounds on germination and seedling growth were elucidated as dammarane type triterpenes. However, less autotoxic effect was recorded by an inhibition under 50% for most of the identified flavonoids. These results suggest that those autotoxic substances may be used as a new bio-herbicide that may contribute to manage the distribution of C. arabica in agronomic field.[Formula: see text].


Assuntos
Cleome/efeitos dos fármacos , Herbicidas/isolamento & purificação , Feromônios/farmacologia , Triterpenos/isolamento & purificação , Flavonóis/isolamento & purificação , Germinação/efeitos dos fármacos , Glicosídeos/isolamento & purificação , Herbicidas/química , Extratos Vegetais/química , Plântula/efeitos dos fármacos , Sementes/química , Triterpenos/química , Triterpenos/farmacologia
17.
Nat Prod Res ; 34(7): 1046-1050, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30580590

RESUMO

Two diketopiperazines were isolated from a culture of the marine-derived actinomycete Streptomyces sp. ZZ446. Their structures were elucidated as maculosin (1) and maculosin-O-α-L-rhamnopyranoside (2) based on their NMR and HRESIMS data, specific rotation, and chemical degradation. Maculosin-O-α-L-rhamnopyranoside (2) is a new diketopiperazine glycoside, a structural class not reported previously from the natural sources. Both compounds showed antimicrobial activity against methicillin-resistant Staphylococcus aureus, Escherichia coli, and Candida albicans with MIC values in a range from 26.0 to 37.0 µg/mL.[Formula: see text].


Assuntos
Dicetopiperazinas/isolamento & purificação , Glicosídeos/isolamento & purificação , Streptomyces/química , Antibacterianos/química , Antibacterianos/isolamento & purificação , Candida albicans/efeitos dos fármacos , Dicetopiperazinas/química , Escherichia coli/efeitos dos fármacos , Glicosídeos/química , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Peptídeos Cíclicos , Piperazinas
18.
Nat Prod Res ; 34(7): 1041-1045, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30580603

RESUMO

The pollen of T. angustifolia, also known as Pu huang in Chinese, has been used for treatment of stranguria, hematuria, dysmenorrhea, metrorrhagia and injuries in China for a long time. Extensive efforts have been directed toward its phytochemical and biological aspects. However, little is known about its anti-nociceptive implication and material basis. This work presented the investigation of the anti-nociceptive effect of Typhae Pollen using an effect-directed fractionation strategy, thereby leading to identification of isorhamnetin-3-O-neohesperidin (1) and typhaneoside (2), together with other minor flavonoid glycoside congeners, as the main anti-nociceptive constituents. This work not only unveils the anti-nociceptive potential of Typhae Pollen, but also establishes a method to enrich and identify the anti-nociceptive constitutes of Typhae Pollen. Moreover, this work is a successful example of effect-directed fractionation strategy, which represents a powerful tool in TCM-based drug discovery and development.


Assuntos
Analgésicos/isolamento & purificação , Flavonoides/farmacologia , Pólen/química , Typhaceae/química , Analgésicos/química , Animais , Fracionamento Químico/métodos , China , Medicamentos de Ervas Chinesas , Flavonoides/química , Flavonoides/isolamento & purificação , Glicosídeos/química , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia
19.
Nat Prod Res ; 34(2): 284-289, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30580615

RESUMO

Baccharoides anthelmintica is the most popular traditional Uighur medicines used for vitiligo. The chemical investigation of the seeds of B. anthelmintica led to the isolation of three new flavonoid glycosides (Vernosides A-C). Their structures were determined by comprehensive analysis of spectroscopic data including 1D and 2D NMR and HRMS data. Vernosides A-C were evaluated for their effects on tyrosinase activity, Vernoside B can enhance tyrosinase activity.


Assuntos
Asteraceae/química , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Sementes/química , Flavonoides/química , Flavonoides/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Estrutura Molecular , Monofenol Mono-Oxigenase/efeitos dos fármacos , Monofenol Mono-Oxigenase/metabolismo
20.
Nat Prod Res ; 34(2): 217-224, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30580625

RESUMO

The chemical composition of Gynostemma burmanicum King ex Chakrav. was investigated for the first time in this study. Nine dammarane glycosides (1‒9) were isolated from the EtOH extract of the aerial parts of G. burmanicum. Their structures were elucidated by 1D and 2D NMR spectroscopic interpretation as well as by chemical studies. The new compounds were 3ß,20S-dihydroxydammar-24-ene-3-O-ß-D-glucopyranosyl-20-O-[ß-D-xylopyranosyl-(1→6)-ß-D-glucopyranoside] (1), 3ß,12ß,20S-trihydroxydammar-24-ene-3-O-ß-D-xylopyranosyl-20-O-[ß-D-xylopyranosyl-(1→6)-ß-D-glucopyranoside] (2), and 12-oxo-3ß,20S-dihydroxydammar-24-ene-3-O-[ß-D-glucopyranosyl(1→2)-ß-D-glucopyranosyl]-20-O-[ß-D-xylopyranosyl-(1→6)-ß-D-glucopyranoside] (3).


Assuntos
Glicosídeos/isolamento & purificação , Gynostemma/química , Triterpenos/isolamento & purificação , Glicosídeos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Extratos Vegetais/química , Saponinas/química , Saponinas/isolamento & purificação , Triterpenos/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA