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1.
Food Chem ; 336: 127674, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32781353

RESUMO

Dried flower buds of Japanese sophora (Sophora japonica) comprising rutinosidase activity were tested in rutinosylation of tyrosol via transglycosylation process from rutin. Optimal conditions for transrutinosylation of tyrosol were 49 mM rutin and 290 mM tyrosol, giving maximum conversion up to 66.4% and 24% yield of isolated and purified rutinoside. The rutinosylation proceeded exclusively on the primary hydroxyl of tyrosol, thus forming rhamnosylated derivative of salidroside. This strict regioselectivity differentiates the sophora biocatalyst from microbial rutinosidases.


Assuntos
Flores/química , Álcool Feniletílico/análogos & derivados , Sophora/química , Flores/metabolismo , Glucosídeos/química , Glucosídeos/metabolismo , Glicosilação , Fenóis/química , Fenóis/metabolismo , Álcool Feniletílico/química , Álcool Feniletílico/metabolismo , Rutina/química , Rutina/metabolismo
2.
J Oleo Sci ; 69(11): 1389-1401, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33132278

RESUMO

The oral route is the most prevalent route of drug administration among various routes. Dapagliflozin is an oral hypoglycemic drug used for lowering the blood glucose level. The objective of this work is to developed and optimized dapagliflozin loaded nanostructured lipid carriers (DG-NLCs) for the improvement of oral delivery. DG-NLCs were prepared by a high-pressure homogenization method (hot) and optimized by Box-Behnken design software using lipid, surfactant, and homogenization cycle as an independent variable. DG-NLCs were evaluated for particle size (Y1), entrapment efficiency (Y2), drug release (Y3). The DG-NLCs were further evaluated for morphology, thermal and X-ray diffraction analysis, ex-vivo intestinal permeation, and stability study. Particle size (nm), entrapment efficiency (%) and drug release (%) of all seventeen formulations were found in the range of 113.71-356.22 nm, 60.43-96.54% and 63.44-83.62% respectively. Morphology of optimized formulation exhibited spherical in shape confirmed by transmission electron microscopy. Thermal and X-ray diffraction analysis of NLCs showed the drug was solubilized and lost the crystallinity. DG-NLCs-opt exhibited dual release pattern initial fast and later sustained-release (90.01±2.01% in 24 h) whereas DG-dispersion showed 31.54±1.87% release in 24 h. Korsmeyer-Peppas model was found to be the best fit model (R2=0.999). The DG-NLCs-opt exhibited significant-high (p < 0.05, 1.293 µg/cm2/h) flux than DG-dispersion (0.2683 µg/cm2/h). Apparent permeation coefficient of DG-NLCs-opt was found to be significantly higher (p < 0.05, 4.14×10-5 cm/min) than DG-dispersion (8.61×10-6 cm/min). The formulation showed no significant changes (p < 0.05) on six months of storage study at 25±2°C/60±5%RH. The finding concluded that quality by design (QbD) based lipid nanocarrier for oral delivery could be a promising approach of dapagliflozin for the management of diabetes.


Assuntos
Compostos Benzidrílicos , Portadores de Fármacos , Composição de Medicamentos , Sistemas de Liberação de Medicamentos/métodos , Liberação Controlada de Fármacos , Glucosídeos , Hipoglicemiantes , Absorção Intestinal , Lipídeos , Nanopartículas , Nanoestruturas , Administração Oral , Compostos Benzidrílicos/química , Compostos Benzidrílicos/metabolismo , Formas de Dosagem , Portadores de Fármacos/química , Desenho de Fármacos , Estabilidade de Medicamentos , Glucosídeos/química , Glucosídeos/metabolismo , Hipoglicemiantes/química , Hipoglicemiantes/metabolismo , Modelos Biológicos , Tamanho da Partícula
3.
J Mol Graph Model ; 100: 107690, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32745925

RESUMO

Coronavirus epidemic 2019 (COVID-19), caused by novel coronavirus (2019-nCoV), is newly increasing worldwide and elevating global health concerns. Similar to SARS-CoV and MERS-CoV, the viral key 3-chymotrypsin-like cysteine protease enzyme (3CLPro), which controls 2019-nCoV duplications and manages its life cycle, could be pointed as a drug discovery target. Herein, we theoretically studied the binding ability of 10 structurally different anthocyanins with the catalytic dyad residues of 3CLpro of 2019-nCoV using molecular docking modelling. The results revealed that the polyacylated anthocyanins, including phacelianin, gentiodelphin, cyanodelphin, and tecophilin, were found to authentically bind with the receptor binding site and catalytic dyad (Cys145 and His41) of 2019-nCoV-3CLpro. Our analyses revealed that the top four hits might serve as potential anti-2019-nCoV leading molecules for further optimization and drug development process to combat COVID-19. This study unleashed that anthocyanins with specific structure could be used as effective anti-COVID-19 natural components.


Assuntos
Antocianinas/química , Antivirais/química , Benzopiranos/química , Betacoronavirus/química , Cisteína Endopeptidases/química , Glucosídeos/química , Inibidores de Proteases/química , Proteínas não Estruturais Virais/química , Sequência de Aminoácidos , Betacoronavirus/enzimologia , Sítios de Ligação , Simulação de Acoplamento Molecular , Ligação Proteica , Domínios e Motivos de Interação entre Proteínas , Estrutura Secundária de Proteína , Alinhamento de Sequência , Relação Estrutura-Atividade , Termodinâmica , Proteínas não Estruturais Virais/antagonistas & inibidores
4.
Food Chem ; 330: 127239, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32540522

RESUMO

Crabapple (Malus prunifolia Willd. Borkh) is a kind of wild apples with many health benefits. However, the utilization of crabapple fruit remains scarce, due to the poor stability of C3G. In this study, C3G loaded nanoparticles were established by chitosan (CS), chitosan oligosaccharides (CSO), and carboxymethyl chitosan (CMC) united with ionic crosslinking agent γ-Polyglutamic acid (PGA) or calcium chloride (CaCl2) to improve the stability of C3G. Results showed that C3G-loaded nanoparticles were exhibited nearly spherical with homogeneous morphology. Particularly, C3G-CMC-CaCl2 nanoparticles exhibited the highest encapsulation efficiency (53.88%) and loading efficiency (5.11%) with preferable particle size (180 nm), good stability (-19 mV) and blood compatibility. C3G-CMC-CaCl2 nanoparticles also revealed the highest releasing ratio (~75%) at pH 5.3 with stability. Present study established the chemical and cell biological basis for further application of C3G-loaded nanoparticles in nutraceutical and functional food fields, extending the application of crabapple in food processing with bioactive enhancement.


Assuntos
Antocianinas/química , Glucosídeos/química , Nanopartículas/química , Quitosana/análogos & derivados , Quitosana/química , Microscopia Eletrônica de Transmissão , Nanopartículas/ultraestrutura , Tamanho da Partícula
5.
Food Chem ; 331: 127305, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32593038

RESUMO

An ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS) method was established and validated for the simultaneous quantification of eight cyanogenic glucosides (CNGs) in agri-food. The eight CNGs were linamarin, lotaustralin, linustatin, neolinustatin, taxiphyllin, amygdalin, dhurrin and prunasin. CNGs were extracted with aqueous methanol and cleaned via solid-phase extraction. Analytes were separated with a C18 column via gradient elution. MS/MS analysis was performed with electrospray ionisation in positive mode. Quantification was performed in multiple reaction monitoring mode. Satisfactory validation results were obtained in terms of linearity, sensitivity, precision and accuracy, matrix effect and stability. The method was applied in typical cyanogenic agri-food. CNGs in cassava, linseed, bamboo, sorghum, apricot, almond and lima bean were analyzed.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Análise de Alimentos/métodos , Glucosídeos/análise , Glucosídeos/química , Nitrilos/química , Espectrometria de Massas em Tandem/métodos , Agricultura , Glucosídeos/isolamento & purificação , Extração em Fase Sólida , Fatores de Tempo
6.
Food Chem ; 331: 127262, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32563799

RESUMO

Stevioside is the main and the sweetest glycoside of stevia plant. It is attractive as a natural sweetener to diabetics and others on carbohydrate-controlled diets. This paper discusses the stability of stevioside under food processing conditions. It was found that stevioside was transformed not only to rubusoside, steviolbioside, steviol monoside and steviol but also to previously unknown stevioside α-anomer and rubusoside α-anomer. Those two identified stevioside transformation products are formed not only during the heating of acidic, neutral and alkaline stevioside standard solutions and stevia leaves suspensions in water and ethanol/water solvents but also during the processing of foods containing stevia. Apart from presenting the new compounds, the paper additionally shows that the recombination of sugar moiety with steviolbioside molecule in MS/ESI source can occur. The effect of molecule recombination in the MS source is known from the literature; however, it has not been reported previously in relation to stevioside derivatives.


Assuntos
Diterpenos de Caurano/química , Manipulação de Alimentos/métodos , Glucosídeos/química , Edulcorantes/química , Cromatografia Líquida de Alta Pressão , Concentração de Íons de Hidrogênio , Hidrólise , Espectrometria de Massas por Ionização por Electrospray , Temperatura
7.
J Chromatogr A ; 1622: 461129, 2020 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-32376025

RESUMO

This work describes the development of capacity orthogonal chromatography (COC), a new technique for simultaneously determining the loading capacity and orthogonality during the construction of two-dimensional (2D) separations. Three steps were required for the construction of a COC based on the correlation between the selectivity factor (α) and both orthogonality and loading capacity. (1) α values of the impurities-target compound were used to normalize the retention of the impurities around the target compound. (2) α values were input into four quadrants of a coordinate system to identify correlations between orthogonality and loading capacity. α values of the impurities must be greater in the first dimension than the second dimension, with iterated analyses performed until an αmax is obtained for the two purification methods. (3) Touch-peak separation using the first-dimensional αmax was performed and the target compound was collected. The co-eluted impurities are further separated in the second dimension. To test the efficiency of this technique, a COC using two methods on a standard C18 column was developed to purify corilagin from pomegranate flower extract. Despite its low abundance, 288 mg of corilagin was obtained by COC and further purified by LH-20 gel chromatography to obtain the compound with an 80.0% recovery and 98.4% purity. Compared to COC, the purity of corilagin independently obtained using the same purification methods and identical loading capacity was poor (60.1% and 61.6%). These results indicate that COC is a useful tool for extending loading capacity in the development of preparative 2D separations.


Assuntos
Flores/química , Glucosídeos/isolamento & purificação , Taninos Hidrolisáveis/isolamento & purificação , Extratos Vegetais/química , Romã (Fruta)/química , Glucosídeos/química , Taninos Hidrolisáveis/química
8.
Food Chem ; 326: 126904, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32413765

RESUMO

In this paper, the interaction of silkworm pupae protein (SPP) with cyanidin-3-O-glucoside (C3G) was studied the protective anthocyanins stability. Characterization experiments suggested that C3G-SPP complexes mainly through hydrophobic interactions, with a decrease in the α-helix content and increases in the ß-sheet and ß-turn contents. Fluorescence results revealed that C3G quenched the intrinsic fluorescence of SPP by static quenching. The highest quenching constant, Kq, was recorded to be1.26 × 1012 M-1s-1 for the SPP preheated at 80 °C. Following the C3G-SPP complexes, the degradation rate constant decreased, and the half-life of C3G was prolonged from 64.81 ± 1.07 to 261.99 ± 13.32 min at 80 °C (p < 0.05). The SPP preheated at 80 °C exhibited the highest binding affinity towards C3G and also effectively increased the thermal and oxidative stability of the C3G. The obtained results suggest that the novel protein proposed in this study could expand the application of anthocyanins as stable, functional food ingredients.


Assuntos
Antocianinas/química , Bombyx/química , Glucosídeos/química , Pupa/química , Animais , Interações Hidrofóbicas e Hidrofílicas , Espectrometria de Fluorescência
9.
J Food Sci ; 85(6): 1717-1724, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32406950

RESUMO

This study evaluated the influence of different fluidized-bed drying temperatures (20, 60, and 100 °C) on the cooking properties, in vitro starch digestibility, and phenolic bioaccessibility of black rice. The results indicated that the formation of fissures in the grains dried at or above 60 °C reduced the physical integrity of the grains after cooking, increasing the starch digestion and the rehydration ratio, and reduced the cooking time, the hardness and adhesiveness. Due to the higher digestibility of grains dried at higher temperatures, an increase in the bioaccessibility of ferulic acid, which was previously associated with the polysaccharides, was observed. Caffeic acid was the only phenolic compound whose levels decreased when the drying temperature increased. At high temperatures and in the gastric phase, cyanidin chalcones were formed due to the deglycosylation of cyanidin-3-O-glucoside. PRACTICAL APPLICATION: The results of this study provide information to the food industry about the effects of different fluidized-bed drying temperatures on the rice structure after cooking and that, consequently, affect the availability of bioactive compounds after digestion and the glycemic index of black rice.


Assuntos
Dessecação/métodos , Oryza/química , Fenóis/química , Amido/química , Antocianinas/química , Antocianinas/metabolismo , Culinária , Digestão , Manipulação de Alimentos , Glucosídeos/química , Glucosídeos/metabolismo , Índice Glicêmico , Humanos , Modelos Biológicos , Oryza/metabolismo , Fenóis/metabolismo , Amido/metabolismo , Temperatura
10.
Food Chem ; 321: 126644, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32247886

RESUMO

Genetics and environment both influence the content of hop (Humulus lupulus L.) aroma compounds. The effects of these two factors on aroma glycosides, which can change the aroma profile of beer over time, were examined in a preliminary study. Twenty-three hop cultivars were grown in the northwestern United States in two locations with distinct terroirs. UPLC-MS/MS analysis of hop cone extracts revealed that growing location had a large effect on hexyl glucoside levels but only a negligible effect on levels of linalyl, raspberry ketone, and 2-phenylethyl glucoside, which were mostly affected by genetic differences. The large terroir effect on hexyl glucoside, which releases a green leaf volatile with a grassy aroma when hydrolyzed, but not on the other aroma glucosides, which have more desirable aromas when hydrolyzed, could have an impact on beer aroma profiles.


Assuntos
Glucosídeos/análise , Glicosídeos/química , Humulus/química , Odorantes/análise , Cromatografia Líquida de Alta Pressão , Cor , Glucosídeos/química , Glicosilação , Estrutura Molecular , Folhas de Planta/química , Espectrometria de Massas em Tandem
11.
PLoS One ; 15(4): e0231815, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32348327

RESUMO

Reducing carbohydrates digestion by having a low glycaemic index (GI) foods has been linked to weight loss. Inhibiting related enzymes is an alternative way to decrease carbohydrate digestion. RCM-107 (Slimming Plus), an eight-herb formula that is modified from RCM-104, indicated significant weight-loss action in clinical trials. However, no published research has studied its mechanism of action on reducing carbohydrate absorption via suppressing the activities of porcine pancreatic alpha-amylase (PPA). In this paper, we used fluorescence PPA inhibition assay to investigate the inhibitory effects of RCM-107 and the individual herbs present in this herbal mixture on amylase activity. Subsequently, molecular docking predicted the key active compounds that may be responsible for the enzyme inhibition. According to our results, both the RCM-107 formula and several individual herbs displayed α-amylase inhibitory effects. Also, marginal synergistic effects of RCM-107 were detected. In addition, alisol B, (-)-epigallocatechin-3-gallate (EGCG) and plantagoside have been predicted as the key active compounds that may be responsible for the α-amylase inhibition effect of RCM-107 according to inter-residue contact analysis. Finally, Glu233, Gln63, His305, Asp300 and Tyr151 are predicted to be markers of important areas with which potential amylase inhibitors would interact. Therefore, our data has provided new knowledge on the mechanisms of action of the RCM-107 formula and its individual herbal ingredients for weight loss, in terms of decreasing carbohydrate digestion via the inhibition of pancreatic alpha-amylase.


Assuntos
Fármacos Antiobesidade/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Obesidade/tratamento farmacológico , alfa-Amilases Pancreáticas/antagonistas & inibidores , Perda de Peso/efeitos dos fármacos , Animais , Fármacos Antiobesidade/química , Metabolismo dos Carboidratos/efeitos dos fármacos , Catequina/análogos & derivados , Catequina/química , Catequina/farmacologia , Colestenonas/química , Colestenonas/farmacologia , Medicamentos de Ervas Chinesas/química , Ensaios Enzimáticos , Flavanonas/química , Flavanonas/farmacologia , Glucosídeos/química , Glucosídeos/farmacologia , Humanos , Simulação de Acoplamento Molecular , Obesidade/metabolismo , alfa-Amilases Pancreáticas/química , alfa-Amilases Pancreáticas/metabolismo , Suínos
12.
Z Naturforsch C J Biosci ; 75(3-4): 121-128, 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32267249

RESUMO

Plants of the Plantago genus are widely used in Turkish folk medicine especially for the treatment of wound, abscess, and inflammation. The aqueous extract and five phenylethanoid glycosides acteoside (1), arenarioside (2), echinacoside (3), isoacteoside (4), and leucosceptoside A (5) isolated from the aerial parts and roots of Plantago holosteum Scop. (Plantaginaceae) were tested for their possible inhibitory activity against hyaluronidase, elastase, and collagenase, related to wound pathogenesis. Even though the aqueous extract prepared from the aerial parts (36.26%) and roots (47.01%) and the isolated compounds acteoside (29.13%), echinacoside (28.73%), and isoacteoside (31.69%) exerted a notable inhibition, arenarioside and leucosceptoside A were found inactive in the hyaluronidase enzyme inhibition test. Similar results were obtained from the collagenase enzyme inhibition test. The aqueous extract prepared from the aerial parts (31.09%) and roots (35.17%), echinacoside (25.13%), and isoacteoside (23.85%) exerted a notable inhibition in this test. However, none of the extracts and isolated compounds displayed elastase enzyme inhibitory activity. The experimental data demonstrated that P. holosteum displayed a remarkable enzyme inhibitory activity against hyaluronidase and collagenase. This paper is the first report regarding the in vitro enzyme inhibitory activity of P. holosteum.


Assuntos
Colagenases/metabolismo , Glicosídeos/farmacologia , Hialuronoglucosaminidase/antagonistas & inibidores , Elastase Pancreática/antagonistas & inibidores , Plantago/química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Glucosídeos/química , Glucosídeos/farmacologia , Glicosídeos/química , Humanos , Medicina Tradicional , Estrutura Molecular , Fenóis/química , Fenóis/farmacologia , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Raízes de Plantas/química , Turquia
13.
Food Chem ; 319: 126515, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32193057

RESUMO

An ultra-high-performance liquid chromatography-diode array detector-mass spectrometry method was developed for characterisation and quantification of anthocyanin components in complex corn-kernel matrices. The anthocyanin profiles and total anthocyanin content (TAC) of mature seeds of five types of anthocyanin-pigmented corn were reported. Internal standard was used to validate the efficiency of extraction and optimise the liquid extraction procedure for anthocyanins. A total of eighteen anthocyanins were identified and quantified. Cyanidin-based glucosides were the major pigments of purple-pericarp sweetcorn (75.5% of TAC) and blue-aleurone maize (91.6%), while pelargonidin-based glucosides composed the main anthocyanins of reddish-purple-pericarp sweetcorn (61.1%) and cherry-aleurone maize (74.6%). Importantly, previous studies reported the presence of acetylated and succinylated anthocyanins in corn kernels; these compounds were found to be artefact pigments, generated during the extraction process. These crucial findings provide the correct anthocyanin profiles of pigmented corns, and emphasise the importance of using acidified solutions for the extraction of corn-based anthocyanins.


Assuntos
Antocianinas/análise , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massas/métodos , Zea mays/química , Glucosídeos/química , Pigmentação
14.
Food Chem ; 320: 126616, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32203835

RESUMO

Egg ovalbumin (OVA) as a prevalent dietary protein and has the potential to serve as a carrier for unstable bioactive compounds, however, understanding their interaction mechanism is the preliminary step. In this work, the interactions between cyanidin-3-O-glucoside (C3G) and OVA in both acidic and neutral pH environment were investigated by spectroscopic methods and molecular docking analysis. The results revealed that fluorescence quenching mechanism of OVA-C3G was predominantly static. The main acting forces were hydrogen bonds and van der Waals forces under varying pH conditions. However, the binding affinity of C3G to OVA was higher in neutral environment than that in acidic condition. The binding of C3G slightly increased the diameter of the complex, resulting in increase of α-helix, decrease of ß-turn, random coil, and total main secondary structure. Moreover, the thermostability of C3G was significantly improved after OVA addition, suggesting its promising application in functional foods.


Assuntos
Antocianinas/química , Glucosídeos/química , Ovalbumina/química , Animais , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Simulação de Acoplamento Molecular , Estrutura Secundária de Proteína , Espectrometria de Fluorescência
15.
Mol Med Rep ; 21(3): 1258-1266, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32016451

RESUMO

Hesperetin is a natural flavonoid with robust antioxidant properties. Our previous study reported that hesperetin can prevent cataract formation. However, an important consideration regarding hesperetin consumption is the limited bioavailability due to its poor solubility. The present study investigated the anti­cataract effects of α­glucosyl hesperidin in vivo and in vitro using a selenite­induced cataract model. SD rats (age, 13 days) were orally administered PBS (0.2 ml) or α­glucosyl hesperidin (200 mg/kg) on days 0, 1 and 2. Sodium selenite was subcutaneously administered to the rats 4 h after the first oral administration on day 0. Antioxidant levels in the lens and blood were measured on day 6. In vitro, human lens epithelial cells were treated with sodium selenite (10 µM) and/or hesperetin (50 or 100 mM) for 24 h and analyzed for apoptosis markers using sub­G1 population and Annexin V­FITC/propidium iodide staining and DNA ladder formation. α­glucosyl hesperidin treatment significantly reduced the severity of selenite­induced cataract. The level of antioxidants was significantly reduced in the selenite­treated rats compared with in the controls; however, they were normalized with α­glucosyl hesperidin treatment. In vitro, hesperetin could significantly reduce the number of cells undergoing apoptosis induced by sodium selenite in human lens epithelial cell lines. Overall, oral consumption of α­glucosyl hesperidin could delay the onset of selenite­induced cataract, at least in part by modulating the selenite­induced cell death in lens epithelial cells.


Assuntos
Antioxidantes/administração & dosagem , Catarata/tratamento farmacológico , Glucosídeos/administração & dosagem , Hesperidina/análogos & derivados , Selenito de Sódio/efeitos adversos , Administração Oral , Animais , Antioxidantes/química , Apoptose/efeitos dos fármacos , Catarata/induzido quimicamente , Catarata/patologia , Morte Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/patologia , Glucosídeos/química , Hesperidina/administração & dosagem , Hesperidina/química , Humanos , Cristalino/efeitos dos fármacos , Cristalino/patologia , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley
16.
Molecules ; 25(3)2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32046151

RESUMO

Manufacturing beer with a high biological value requires identifying new methods for increasing the health-enhancing compounds level. The aim of this study was to increase the biological value of beer by adding antioxidant-rich eggplant (Solanum melongena L.) peel extract (EPE). The total phenolic content (TPC), total flavonoid content (TFC), and total monomeric anthocyanin content (TMA) were determined. Moreover, the antioxidant activity was evaluated by different radical scavenging assays. The addition of different levels of EPE resulted in a significant increase of TPC and TFC of beer samples from 0.426 to 0.631 mg GAE/mL, and from 0.065 to 0.171 mg CE/mL, respectively. The EPE-supplemented beer samples developed a reddish color because of the presence of anthocyanin pigments. The TMA content of beer varied from 0.011 to 0.083 mg D3G/mL with the level of added EPE. The HPLC analysis indicated that the anthocyanins prevailing in the eggplant peels were delphinidin-3-rutinoside, delphininidin-3-glucoside and delphinidin-3-rutinoside-5-glucoside. The radical scavenging assays indicated a linear increase of the antioxidant activity following EPE addition, without altering the physicochemical parameters of the beer. These results are promising for using the EPE as a functional ingredient for beer production.


Assuntos
Cerveja , Extratos Vegetais/química , Solanum melongena/química , Antocianinas/química , Antioxidantes/química , Cromatografia Líquida de Alta Pressão/métodos , Cor , Flavonoides/química , Hipersensibilidade Alimentar , Frutas/química , Glucosídeos/química , Fenóis/química , Pigmentos Biológicos/química
17.
Food Chem ; 317: 126418, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32087512

RESUMO

The influence of encapsulation with caseins on the stability of cyanidin 3-O-glucoside (C3G) was investigated. The modified casein nanoparticles (MCs) prepared at pH 5.5 after heated at 80 °C for 30 min was applied to encapsulate C3G. The diameter of nanoparticle (MCs-C3G) was 110 ± 0.31 nm and zeta-potential was -8.83 ± 0.52 mV. The molecular weight of α-casein (32 kDa) and ß-casein (25 kDa) increased along with the encapsulation of C3G. The interactions of MCs with C3G were examined at pH 6.3 by fluorescence spectroscopy and IR spectroscopy. MCs encapsulated C3G mainly via the hydrophobic interaction. The secondary structures of caseins were changed along with the combination of C3G, with a decreasing in α-helix, turn random, and coil structure, as well as increased ß-sheet. In addition, the MCs-C3G interaction appeared to have a positive effect on the thermal, oxidation and photo stability of C3G.


Assuntos
Antocianinas/química , Caseínas/química , Glucosídeos/química , Nanopartículas/química , Estabilidade de Medicamentos , Temperatura Alta , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula , Estrutura Secundária de Proteína
18.
Food Chem ; 316: 126364, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32058190

RESUMO

Heat- and ultrasound-assisted extractions of anthocyanins from jabuticaba epicarp were optimized and the colouring potential of the developed extract was tested on macarons. The independent variables time (t), solvent concentration (S), and temperature (T) or power (P) were combined in a five-level central composite design coupled with response surface methodology. The delphinidin-3-O-glucoside and cyanidin-3-O-glucoside levels monitored by HPLC-DAD-ESI/MS were used as response criteria. The developed models were successfully fitted to the experimental data and used to determine optimal extraction conditions. HAE was the most efficient method yielding 81 ± 2 mg/g extract under optimal conditions (t = 21.8 min, T = 47.1 °C and S = 9.1% ethanol, v/v). Macarons were then produced using the optimized anthocyanin-rich colouring extract and their colour parameters and nutritional profile were monitored during shelf-life. The obtained results provided useful information for the development of anthocyanin-rich extracts from a bio-waste with potential use as natural food colorants.


Assuntos
Antocianinas/química , Corantes de Alimentos/química , Myrtaceae/química , Extratos Vegetais/química , Pão , Cromatografia Líquida de Alta Pressão , Cor , Glucosídeos/química , Temperatura Alta , Espectrometria de Massas por Ionização por Electrospray , Ultrassom
19.
Food Chem ; 317: 126359, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32097820

RESUMO

High content of steviol glycosides in stevia leaves is a cause of their high popularity as. a natural sweetener of various sugar-free food products. Stevioside (13-[(2-O-ß-d-glucopyranosyl-ß-d-glucopyranosyl)oxy]-ent-kaur-16-en-19-oic acid ß-d-glucopyranosyl ester) is one of the main steviol glycosides in stevia leaves known for its hydrolytic instability responsible for the formation of simple steviol glucosides (steviolbioside, rubusoside, steviol monoside) and steviol. However, the formation of hydroxy and alkoxy adducts of stevioside and of its hydrolysis products has not yet been reported. The performed experiments prove that water and alkoxy adducts are formed not only during temperature processing of stevioside but also of stevia and stevia-containing food products. Their quantities depend on environment pH, water concentration and food composition. Although they are formed in small amounts their biological activity is unknown and should be recognized.


Assuntos
Diterpenos de Caurano/química , Glucosídeos/química , Stevia/química , Análise de Alimentos , Concentração de Íons de Hidrogênio , Hidrólise , Metanol/química , Folhas de Planta/química , Folhas de Planta/metabolismo , Stevia/metabolismo , Edulcorantes/química , Temperatura , Água/química
20.
J Agric Food Chem ; 68(9): 2783-2794, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32017557

RESUMO

Pyranoanthocyanins are the important color and functional compounds in red wine. Six common kinds of pyranoanthocyanins were synthesized through the reaction of malvidin-3-O-glucoside (Mv-3-gluc) with acetone, pyruvic acid, p-cumaric acid, caffeic acid, ferulic acid, and sinapid acid, respectively, and their pH (1.0-11.0), SO2 (0-250 ppm), and thermo (50-98 °C) stabilities and antioxidant activities were comparatively studied. Results showed that the six pyranoanthocyanins all exhibited higher pH and SO2 color stability than Mv-3-gluc, especially vitisin-A with a carboxy group on the D ring. The six pyranoanthocyanins also showed much more thermostability than Mv-3-gluc, especially methylpyranomv-3-gluc. The degradation kinetics of Mv-3-gluc and its derivatives all fitted to a first-order reaction. Moreover, pinotin-A with the o-dihydroxyl group on the E ring presented the strongest antioxidant capability, as evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric ion reducing power (FRAP) assays. Additionally, the increase in the number of rings is beneficial to the improvement of the DPPH radical scavenging ability of anthocyanins.


Assuntos
Antocianinas/química , Antioxidantes/química , Glucosídeos/química , Estabilidade de Medicamentos , Concentração de Íons de Hidrogênio , Cinética , Estrutura Molecular
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