Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.822
Filtrar
1.
Anticancer Res ; 40(10): 5701-5706, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32988895

RESUMO

BACKGROUND/AIM: The simultaneous increase of antioxidant CAT (catalase) enzyme and plasma MDA (malonidialdehyde) concentrations versus the numeric rating scale (NRS) pain score following surgery is unknown. Patients and Methods: The study included 114 patients with gallstone disease and 29 patients in the cancer group. RESULTS: Following surgery, the plasma CAT concentrations increased and plasma MDA concentrations decreased in all patients and especially in cancer patients. The linear mixed model time-effect was statistically significant in CAT and MDA (p<0.001 and p=0.02, respectively). In addition, a significant correlation between NRS pain score values and plasma MDA median concentrations in cancer patients was identified (r=0.430, p<0.001). CONCLUSION: The plasma MDA concentrations decreased and CAT concentrations increased significantly in all patients and especially in cancer patients following surgery. The simultaneous increase of antioxidant CAT enzyme with the decrease of plasma MDA may be an important ROS inhibiting mechanism to help patients return to normal antioxidant-oxidant status.


Assuntos
Catalase/sangue , Cálculos Biliares/sangue , Malondialdeído/sangue , Neoplasias/sangue , Dor/sangue , Antioxidantes/metabolismo , Feminino , Cálculos Biliares/patologia , Cálculos Biliares/cirurgia , Glutationa Peroxidase/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/patologia , Neoplasias/cirurgia , Estresse Oxidativo/genética , Dor/patologia , Dor/cirurgia , Medição da Dor , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/sangue
2.
Am J Physiol Regul Integr Comp Physiol ; 319(2): R203-R210, 2020 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-32609535

RESUMO

Canids are a morphological and physiological diverse group of animals, with the most diversity found within one species, the domestic dog. Underlying observed morphological differences, there must also be differences at other levels of organization that could lead to elucidating aging rates and life span disparities between wild and domestic canids. Furthermore, small-breed dogs live significantly longer lives than large-breed dogs, while having higher mass-specific metabolic rates and faster growth rates. At the cellular level, a clear mechanism underlying whole animal traits has not been fully elucidated, although oxidative stress has been implicated as a potential culprit of the disparate life spans of domestic dogs. We used plasma and red blood cells from known aged domestic dogs and wild canids, and measured several oxidative stress variables: total antioxidant capacity (TAC), lipid damage, and enzymatic activities of catalase, superoxide dismutase, and glutathione peroxidase (GPx). We used phylogenetically informed general linear mixed models and nonphylogenetically corrected linear regression analysis. We found that lipid damage increases with age in domestic dogs, whereas TAC increases with age and TAC and GPx activity increases as a function of age/maximum life span in wild canids, which may partly explain longer potential life spans in wolves. As body mass increases, TAC and GPx activity increase in wild canids, but not domestic dogs, highlighting that artificial selection may have decreased antioxidant capacity in domestic dogs. We found that small-breed dogs have significantly higher circulating lipid damage compared with large-breed dogs, concomitant to their high mass-specific metabolism and higher growth rates, but in opposition to their long life spans.


Assuntos
Peso Corporal/fisiologia , Longevidade/fisiologia , Estresse Oxidativo/fisiologia , Animais , Canidae , Catalase/sangue , Cães , Feminino , Glutationa Peroxidase/sangue , Masculino , Oxirredução , Filogenia , Superóxido Dismutase/sangue
3.
Nutrients ; 12(7)2020 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-32708526

RESUMO

SARS-CoV-2 infections underlie the current coronavirus disease (COVID-19) pandemic and are causative for a high death toll particularly among elderly subjects and those with comorbidities. Selenium (Se) is an essential trace element of high importance for human health and particularly for a well-balanced immune response. The mortality risk from a severe disease like sepsis or polytrauma is inversely related to Se status. We hypothesized that this relation also applies to COVID-19. Serum samples (n = 166) from COVID-19 patients (n = 33) were collected consecutively and analyzed for total Se by X-ray fluorescence and selenoprotein P (SELENOP) by a validated ELISA. Both biomarkers showed the expected strong correlation (r = 0.7758, p < 0.001), pointing to an insufficient Se availability for optimal selenoprotein expression. In comparison with reference data from a European cross-sectional analysis (EPIC, n = 1915), the patients showed a pronounced deficit in total serum Se (mean ± SD, 50.8 ± 15.7 vs. 84.4 ± 23.4 µg/L) and SELENOP (3.0 ± 1.4 vs. 4.3 ± 1.0 mg/L) concentrations. A Se status below the 2.5th percentile of the reference population, i.e., [Se] < 45.7 µg/L and [SELENOP] < 2.56 mg/L, was present in 43.4% and 39.2% of COVID samples, respectively. The Se status was significantly higher in samples from surviving COVID patients as compared with non-survivors (Se; 53.3 ± 16.2 vs. 40.8 ± 8.1 µg/L, SELENOP; 3.3 ± 1.3 vs. 2.1 ± 0.9 mg/L), recovering with time in survivors while remaining low or even declining in non-survivors. We conclude that Se status analysis in COVID patients provides diagnostic information. However, causality remains unknown due to the observational nature of this study. Nevertheless, the findings strengthen the notion of a relevant role of Se for COVID convalescence and support the discussion on adjuvant Se supplementation in severely diseased and Se-deficient patients.


Assuntos
Betacoronavirus , Infecções por Coronavirus/mortalidade , Pneumonia Viral/mortalidade , Selênio/deficiência , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Infecções por Coronavirus/epidemiologia , Estudos Transversais , Feminino , Alemanha/epidemiologia , Glutationa Peroxidase/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Estado Nutricional , Pandemias , Pneumonia Viral/epidemiologia , Prognóstico , Selênio/sangue , Selenoproteína P/sangue
4.
PLoS One ; 15(6): e0234253, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32555583

RESUMO

Oxidative status of maternal blood represents an important parameter of pregnancy that is involved in both, regulation of physiological processes and (if significantly altered) development of different pregnancy complications. Inherited thrombophilias represent genetic disorders that increase the risk of thromboembolism in pregnancy. Little is known about the impact of thrombophilia on the oxidative status of maternal blood. In this study, we analyzed oxidative status of blood of 56 women with pregnancies burdened by inherited thrombophilias. The status was established at three different trimesters using biochemical assays and electrochemical measurements, and it was compared to 10 age- and trimester-matching controls. Activities of superoxide dismutase, catalase, and glutathione reductase in the 1st and the 2nd trimester of thrombophilic pregnancy were lower than controls. Also, there was less oxidation in the plasma, according to higher concentration of reduced thiols and lower oxidation-reduction potential. Therefore, it appears that thrombophilic mothers do not experience oxidative stress in the circulation in the first two trimesters. However, the rise in GPx, GR and SOD activities in the 3rd trimester of thrombophilic pregnancy implies that the risk of oxidative stress is increased during the late pregnancy. These results are important for developing antioxidative treatment that could tackle thrombophilia-related pregnancy complications.


Assuntos
Complicações Hematológicas na Gravidez/sangue , Complicações Hematológicas na Gravidez/metabolismo , Trombofilia/sangue , Trombofilia/metabolismo , Adulto , Estudos de Coortes , Eritrócitos/enzimologia , Feminino , Glutationa Peroxidase/sangue , Humanos , Oxirredução , Oxirredutases/sangue , Gravidez , Complicações Hematológicas na Gravidez/enzimologia , Trombofilia/enzimologia
5.
Medicine (Baltimore) ; 99(26): e20872, 2020 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-32590792

RESUMO

PURPOSE: Oxidative stress has been shown to reflect on the development of sepsis and disease severity. In the present study, we evaluated the effects of increased levels of oxidative stress and decreased antioxidant coactivity in patients with sepsis, and the importance of oxidative stress on treatment outcomes. METHODS: Biomarkers of oxidative stress (thiobarbituric acid-reactive substances [TBARS]) and antioxidant capacity (glutathione peroxidase [GPx] and glutathione content [thiol]) were prospectively evaluated along with biochemical and clinical data in 100 patients with sepsis on days 1, 4, and 7 after admission. RESULTS: The TBARS level of the non-survivor group was significantly higher than that of the survivor group on day 1 and day 4 and negatively correlated with thiol upon admission. However, thiol was positively correlated with lactate concentration. The TBARS and lactate levels upon admission were independent predictors of fatality. CONCLUSIONS: We conclude that a TBARS cut-off value of 18.30 µM can be used to predict fatality, and an increase in the TBARS concentration by 1 µM will increase the fatality rate by 0.94%. In the panel of biomarkers, the TBARS assay can be considered as a prognostic biomarker for the treatment of patients with sepsis.


Assuntos
Biomarcadores/análise , Estresse Oxidativo/fisiologia , APACHE , Adulto , Idoso , Análise de Variância , Área Sob a Curva , Biomarcadores/sangue , Feminino , Glutationa Peroxidase/análise , Glutationa Peroxidase/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Curva ROC , Compostos de Sulfidrila/análise , Compostos de Sulfidrila/sangue , Sobreviventes/estatística & dados numéricos , Substâncias Reativas com Ácido Tiobarbitúrico/análise
6.
Medicine (Baltimore) ; 99(17): e19938, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32332675

RESUMO

The pathophysiology of alcohol use disorder (AUD) is not totally clear. The aim of this study was to investigate the serum levels of brain-derived neurotrophic factor (BDNF) and oxidative stress markers in AUD patients during alcohol detoxification. Evaluation of changes in BDNF, glutathione peroxidase (GPX), catalase, superoxide dismutase, thiobarbituric acid reactive substances, 8-hydroxy 2'-deoxyguanosine, PCC and S100B were carried out.14 AUD inpatients and 20 healthy control subjects were recruited for this study. The serum BDNF, S100B and oxidative stress markers were measured with assay kits.Serum levels of catalase, GPX, PCC and 8-hydroxy 2'-deoxyguanosine were significantly higher in the AUD group subjects than in the controls (P < .05). However, BDNF levels were lower in the AUD group than in the controls (P < .05). After alcohol detoxification treatment, the GPX levels in the AUD group dropped (P < .05) and the BDNF levels rose (P < .05).The results suggest that serum BDNF and GPX levels might be state biomarkers for AUD patients undergoing alcohol detoxification.


Assuntos
Alcoolismo/sangue , Alcoolismo/tratamento farmacológico , Fator Neurotrófico Derivado do Encéfalo/análise , Glutationa Peroxidase/análise , Inativação Metabólica/fisiologia , Adulto , Alcoolismo/fisiopatologia , Biomarcadores/análise , Biomarcadores/sangue , Fator Neurotrófico Derivado do Encéfalo/sangue , Feminino , Glutationa Peroxidase/sangue , Humanos , Inativação Metabólica/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade
7.
Mutat Res ; 850-851: 503152, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32247560

RESUMO

The aim of the present study was to assess the oxidative stress level and chromosomal damage induced by occupational exposure to low dose ionizing radiation (LDIR). Two hundred and eighteen hospital workers occupationally exposed to LDIR were included in this study, along with 118 healthy age- and gender-comparable controls. Occupational dosimetry records were collected over the last year and revealed that the accumulated annual dose for each hospital worker was below the permissible limit of the International Commission on Radiological Protection (ICRP). The individuals' oxidative and antioxidative status were determined by measuring the activities of copper zinc-superoxide dismutase (CuZn-SOD), glutathione peroxidase (GSH-Px), catalase (CAT) enzymes, and the levels of malondialdehyde (MDA) in erythrocytes. The effect of radiation on chromosomal integrity was measured by the frequency of micronuclei (MN) formation using the cytokinesis block technique. Our results showed that the activities of CuZn-SOD and CAT enzymes and MDA levels observed in the hospital workers were higher than those in the controls (p < 0.05). We did not find significant difference in GSH-Px enzyme activity between the two groups (p = 0.247). A higher frequency of MN was found in exposed groups than in the controls [3(1-5) ‰ versus 2(0.75-4) ‰; p<0.001]. The difference was significant for males (p = 0.012), but not females (p = 0.14). Multiple linear regression analysis showed differences in the oxidant activities and MN frequency between hospital workers and controls adjusted for age, gender, smoking status and drinking status. Correlation analysis indicated that the frequency of MN was positively associated with MDA levels (p < 0.05). Altogether, these results support the detrimental effects of chronic low dose radiation in humans, which involves the induction of oxidative stress and chromosomal damage.


Assuntos
Antioxidantes/metabolismo , Aberrações Cromossômicas/efeitos da radiação , Exposição Ocupacional/efeitos adversos , Estresse Oxidativo/efeitos da radiação , Adulto , Antioxidantes/efeitos da radiação , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Hospitais , Humanos , Masculino , Malondialdeído/sangue , Registros Médicos , Pessoa de Meia-Idade , Doses de Radiação , Radiação Ionizante , Radiometria , Superóxido Dismutase-1/sangue
8.
PLoS One ; 15(3): e0230374, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32210468

RESUMO

Oxidative stress is suggested to be the crucial factor in diabetes mellitus type 2 (DM2) pathogenesis and in the development of diabetic complications. Patients with DM2 may be more susceptible to infections due to hyperglycaemia-induced virulence of various microorganisms. Several studies pointed that Epstein-Barr virus (EBV) infection is associated with reactive oxygen species (ROS) production and/or activation of signalling pathways connected with ROS. The present study analyzed serum activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in DM2 patients with and without EBV infection. Blood and saliva were collected from 120 patients with DM2. EBV DNA was detected in the saliva using nested-PCR technique. Spectrophotometric methods were implemented to determine serum GPx and SOD activity with the use of diagnostic kits produced by Randox Laboratories. GPx and SOD activity was decreased in diabetic patients, with the lowest values in DM2 EBV-positive patients. There was correlation between GPx and SOD activity-with increased value of GPx, SOD activity was also rised. In patients with DM2 history longer than 10 years as well as in DM2 patients with obesity, antioxidant enzymes activity was decreased. Determination of examined parameters may be useful in diabetic patients with EBV infection and could be important prognostic factor.


Assuntos
Diabetes Mellitus Tipo 2/sangue , Infecções por Vírus Epstein-Barr/sangue , Glutationa Peroxidase/sangue , Herpesvirus Humano 4/isolamento & purificação , Superóxido Dismutase/sangue , Adulto , DNA Viral/isolamento & purificação , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/imunologia , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/imunologia , Infecções por Vírus Epstein-Barr/virologia , Estudos de Viabilidade , Feminino , Glutationa Peroxidase/metabolismo , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo/imunologia , Prognóstico , Espécies Reativas de Oxigênio/imunologia , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/imunologia , Superóxido Dismutase/metabolismo , Adulto Jovem
9.
Oxid Med Cell Longev ; 2020: 1079129, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32064020

RESUMO

Inflammation and oxidative stress are pivotal mechanisms for the pathogenesis of ischemia and reperfusion injury (IRI). Vagus nerve stimulation (VNS) may participate in maintaining oxidative homeostasis and response to external stimulus or injury. We investigated whether the in vivo VNS can protect the liver from IRI. In this study, hepatic IRI were induced by ligating the vessels supplying the left and middle lobes of the liver, which underwent 1 h occlusion followed with 24 h reperfusion. VNS was initiated 15 min after ischemia and continued 30 min. Hepatic function, histology, and apoptosis rates were evaluated after 24 h reperfusion. Compared with the IRI group, VNS significantly improved hepatic function. The protective effect was accompanied by a reduction in histological damage in the ischemic area, and the apoptosis rate of hepatocytes has considerable reduction. To find the underlying mechanism, proteomic analysis was performed and differential expression of glutathione synthetase (GSS) and glutathione S-transferase (GST) was observed. Subsequently, test results indicated that VNS upregulated the expression of mRNA and protein of GSS and GST. Meanwhile, VNS increased the plasma levels of glutathione and glutathione peroxidases. We found that VNS alleviated hepatic IRI by upregulating the antioxidant glutathione via the GSS/glutathione/GST signaling pathway.


Assuntos
Glutationa/sangue , Hepatócitos/metabolismo , Hepatopatias/terapia , Traumatismo por Reperfusão/terapia , Estimulação do Nervo Vago , Animais , Antioxidantes/metabolismo , Apoptose/genética , Citocinas/metabolismo , Glutationa/biossíntese , Glutationa/metabolismo , Glutationa Peroxidase/sangue , Glutationa Sintase/genética , Glutationa Sintase/metabolismo , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Hepatócitos/enzimologia , Fígado/enzimologia , Fígado/lesões , Fígado/metabolismo , Fígado/patologia , Hepatopatias/enzimologia , Hepatopatias/metabolismo , Hepatopatias/patologia , Masculino , Estresse Oxidativo , Proteômica , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/enzimologia , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Transdução de Sinais/genética
10.
Int J Sports Physiol Perform ; 15(6): 874-883, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32023544

RESUMO

PURPOSE: To investigate the effects of napping after partial sleep deprivation (PSD) on reaction time, mood, and biochemical response to repeated-sprint exercise in athletes. METHODS: Nine male judokas performed 4 test sessions in a counterbalanced and randomized order. Participants accomplished 1 control session after a normal sleep night (NSN) and 3 after PSD with (1) no nap, (2) âˆ¼20-min nap (N20), and (3) âˆ¼90-min nap (N90) opportunities. Test sessions included the running-based anaerobic sprint test, reaction time, Hooper index, and Epworth Sleepiness Scale. Muscle-damage biomarkers and antioxidant status were evaluated before and after exercise. RESULTS: PSD decreased maximum (P < .001, d = 1.12), mean (P < .001, d = 1.33), and minimum (P < .001, d = 1.15) powers compared with NSN. However, N20 and N90 enhanced maximum power compared with PSD (P < .05, d = 0.54; P < .001, d = 1.06, respectively). Minimum power and mean power increased only after N90 (P < .001, d = 1.63; P < .001, d = 1.16, respectively). Epworth Sleepiness Scale increased after PSD (P < .001, d = 0.86) and decreased after N20 (P < .001, d = 1.36) and N90 (P < .001, d = 2.07). N20 reduced multiple-choice reaction time (P < .001, d = 0.61). Despite performance decrement, PSD increased postexercise aspartate aminotransferase (P < .001, d = 4.16) and decreased glutathione peroxidase (P < .001, d = 4.02) compared with NSN. However, the highest performances after N90 were accompanied with lesser aspartate aminotransferase (P < .001, d = 1.74) and higher glutathione peroxidase (P < .001, d = 0.86) compared with PSD. CONCLUSIONS: Napping could be preventive against performance degradation caused by sleep loss. A short nap opportunity could be more beneficial when the subsequent effort is brief and requires frequent decision making. However, a longer nap opportunity could be preventive against muscle and oxidative damage, even for higher performances.


Assuntos
Desempenho Atlético/fisiologia , Músculo Esquelético/metabolismo , Estresse Oxidativo , Privação do Sono/fisiopatologia , Sono/fisiologia , Adolescente , Afeto/fisiologia , Aspartato Aminotransferases/sangue , Biomarcadores/sangue , Glutationa Peroxidase/sangue , Humanos , Ácido Láctico/sangue , Masculino , Percepção/fisiologia , Esforço Físico/fisiologia , Tempo de Reação/fisiologia , Fatores de Tempo , Adulto Jovem
11.
BMC Pharmacol Toxicol ; 21(1): 10, 2020 02 10.
Artigo em Inglês | MEDLINE | ID: mdl-32041665

RESUMO

BACKGROUND: Hyperuricemia is an abnormal increase in uric acid levels in the blood. It is the cause of gout that manifested by inflammatory arthritis and painful disable. Therefore, current study evaluated the potential ameliorative impact of Lesinurad and Allopurinol on the kidneys of hyperuricemic mice at the biochemical, molecular and cellular levels. METHODS: Lesinurad and allopurinol alone or in combination were orally administered to hyperuricemic and control mice for seven consecutive days. Levels of uric acid and blood urea nitrogen, along with antioxidants and inflammatory cytokines (IL-1ß and TNF-α) were measured in the serum. The mRNA expression of mouse urate anion transporter-1, glucose transporter 9, organic anion transporters, in renal tissues were examined using quantitative real time PCR. Simultaneously, the immunoreactivity of transforming growth factor-beta 1 was examined immunohistochemically. RESULTS: Lesinurad and allopurinol administration resulted in significant decrease in serum levels of uric acid, blood urea nitrogen, xanthine oxidase activity, catalase, glutathione peroxidase and inflammatory cytokines (IL-1ß and TNF-α) reported in hyperuricemic mice. Both partially reversed oxonate-induced alterations in renal mURAT-1, mGLUT-9, mOAT-1 and mOAT-3 expressions, as well as alterations in the immunoreactivity of TGF- ß1, resulting in the increase of renal uric acid secretion and excretion. The combined administration of lesinurad and ALP restored all altered parameters in a synergistic manner, improving renal function in the hyperuricemic mouse model employed. CONCLUSION: This study confirmed synergistic ameliorative hypouricemic impact of both lesinurad and allopurinol in the treatment of hyperuricemia in mice at the biochemical, molecular and cellular levels.


Assuntos
Alopurinol/uso terapêutico , Supressores da Gota/uso terapêutico , Hiperuricemia/tratamento farmacológico , Tioglicolatos/uso terapêutico , Triazóis/uso terapêutico , Alopurinol/farmacologia , Animais , Nitrogênio da Ureia Sanguínea , Catalase/sangue , Sinergismo Farmacológico , Glutationa Peroxidase/sangue , Supressores da Gota/farmacologia , Hiperuricemia/sangue , Hiperuricemia/genética , Hiperuricemia/metabolismo , Interleucina-1beta/sangue , Rim/efeitos dos fármacos , Rim/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Camundongos , Tioglicolatos/farmacologia , Triazóis/farmacologia , Fator de Necrose Tumoral alfa/sangue , Ácido Úrico/sangue , Xantina Oxidase/sangue , Xantina Oxidase/metabolismo
12.
J Sports Med Phys Fitness ; 60(4): 669-674, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32043346

RESUMO

BACKGROUND: High-intensity intermittent training (HIIT) is an emerging strategy for controlling blood pressure (BP) requiring intermittent exercise. However, few studies were focused on clinical test or related mechanisms. Here we compared the detailed aspects of HIIT on rat blood pressure control and explored its possible molecular mechanisms. METHODS: Thirty-six spontaneous hypertensive rats (SHR) were recruited to complete 8 weeks of different training pattern using treadmill. Measurements of BP, bradycardia reflex, tachycardia reflex, plasma oxidative stress biomarkers and protein expression were acquired at the end of training. RESULTS: After the 8-week training, HIIT can significantly downregulate the rest heart rate (HR) and blood pressure of SHR. The bradycardia reflex induced by phenylephrine and tachycardia response to sodium nitroprusside (SNP) were both improved in the HIIT group compared with control group. By testing the plasma metabolites, we found no statistically alteration on levels of malondialdehyde (MDA) or superoxide dismutase (SOD). However, HIIT increased the plasma glutathione peroxidase (GSH-Px) activity. Besides, HIIT attenuated the vasoconstriction induced by norepinephrine while has little effect on potassium chloride stimulation. Similarly, the sensitivity of vasorelaxation induced by SNP was upregulated after HIIT. Finally, we identified a decrease of of calcium channel CaV 1.2 on blood vessel in HIIT group. CONCLUSIONS: HIIT provides a better control of BP and higher sensitivity to vasorelaxation, which may be related to higher GSH-Px activity and lower CaV 1.2 expression.


Assuntos
Artérias/fisiopatologia , Canais de Cálcio/metabolismo , Glutationa Peroxidase/sangue , Treinamento Intervalado de Alta Intensidade , Hipertensão/fisiopatologia , Animais , Barorreflexo , Pressão Sanguínea , Cálcio/metabolismo , Canais de Cálcio/genética , Teste de Esforço , Feminino , Frequência Cardíaca , Humanos , Hipertensão/sangue , Hipertensão/metabolismo , Masculino , Malondialdeído/metabolismo , Oxirredução , Estresse Oxidativo , Condicionamento Físico Animal , Ratos , Ratos Endogâmicos SHR , Superóxido Dismutase/metabolismo
13.
J Nutr ; 150(3): 483-491, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31773160

RESUMO

BACKGROUND: The metabolic function of selenoprotein V (SELENOV) remains unknown. OBJECTIVES: Two experiments were conducted to determine effects of the Selenov knockout (KO) on selenium concentration and mRNA, protein, and/or activity of 4 major selenoproteins [glutathione peroxidase (GPX) 1, GPX4, thioredoxin reductase-1 (TXNRD1), and selenoprotein P (SELENOP)] in the serum, liver, testis, and/or white adipose tissue (WAT) of mice fed different dietary selenium and fat concentrations. METHODS: In Experiment (Expt) 1, 40 KO and 40 wild-type (WT) mice (males, 8 wk old) were fed (n = 10/genotype) a casein-sucrose basal diet plus 0, 0.3, 1, or 3 mg Se/kg (as sodium selenite) for 32 wk . In Expt 2, 20 KO and 20 WT mice (males, 8 wk old) were fed (n  = 10/genotype) a normal-fat diet (NF; 10% calories from fat) or a high-fat diet (HF; 60% calories from fat) for 19 wk. RESULTS: In Expt 1, the KO caused consistent or substantial decreases (P < 0.05) of mRNA amounts of Gpx1, Txnrd1, and Selenop in the testis (≤52%), but selenium concentrations (19-29%) and GPX activities (≤ 50%) were decreased in the liver across different dietary selenium concentrations . Hepatic and testis GPX1 protein was elevated (≤31%) and decreased (≤45%) by the KO, respectively. In Expt 2, the genotype and dietary fat intake exerted interaction effects ( P < 0.05) on Gpx1 mRNA amounts in the WAT; Gpx1, Txnrd1, and Selenop mRNA amounts and TXNRD activities in the testis; and selenium concentrations in the serum and liver. However, these 2 treatments produced largely independent or additive effects (P < 0.05) on the GPX1 and SELENOP protein amounts in the liver and testis (up to ± 50% changes). CONCLUSIONS: The KO-mediated changes in the tissue selenium concentrations and functional expression of 3 major selenoproteins implied potential for SELENOV in regulating body selenium metabolism in the mouse.


Assuntos
Dieta , Gorduras na Dieta/administração & dosagem , Selênio/administração & dosagem , Selenoproteínas/fisiologia , Tecido Adiposo Branco/metabolismo , Animais , Peso Corporal , Glutationa Peroxidase/sangue , Glutationa Peroxidase/metabolismo , Fígado/enzimologia , Fígado/metabolismo , Masculino , Camundongos , Camundongos Knockout , RNA Mensageiro/genética , Selênio/sangue , Selênio/metabolismo , Selenoproteínas/genética , Testículo/enzimologia , Testículo/metabolismo
14.
J Ethnopharmacol ; 250: 112435, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-31785384

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Albizia procera L. (Leguminosae) commonly known as Konda vagai in Tamil, is used for the treatment of stomach and intestinal disorders. A decoction of the bark is prescribed for rheumatism and haemorrhage. Traditionally, literature claims Albizia procera as a drug to have antirheumatic properties and hence used by Tribal for the management of chronic rheumatism. Consequently, the present study has been undertaken to illustrate the beneficial outcome of Albizia procera in adjuvant induced arthritic rat model with respect to its antioxidant and anti-inflammatory activities. AIM OF THE STUDY: The present study is aimed to investigate the oxidative stress and the expression of inflammatory markers in arthritic rats treated with ethanolic bark extract of Albizia procera. MATERIALS AND METHODS: Ethanolic bark extract was characterized by HPTLC analysis. Acute oral toxicity study was performed according to the OECD test guideline 423 - Acute toxic class method. The anti-inflammatory effect of ETBE (100, 200 mg/kg/day/p.o.) was evaluated in complete Freund's adjuvant induced arthritic rats using diclofenac as positive control (0.3 mg/kg/day/p. o.). Plasma levels of interleukins TNF- α, IFN-α, IL-2, IL-6, myeloperoxidase and Cathepsin D levels were measured to assess the inflammatory effect of ETBE extract of Albizia procera. Further, the effect of ETBE on superoxide dismutase (SOD), glutathione peroxidase (GPX), reduced glutathione (GSH) and lipid peroxidation (LPO) were assessed in plasma. RESULTS: HPTLC analysis showed the presence of 0.57% w/w of biochanin-A in ETBE. ETBE did not show any toxic signs up to 2000 mg/kg body weight. It exhibited the significant anti-inflammatory and antioxidant potential and did not show mortality up to 2000 mg/kg body weight. ETBE treatment significantly reduced the levels of TNF- α, IFN-α, IL-2, IL-6 and myeloperoxidase, and increased cathepsin D levels compared to vehicle treated animals. SOD, GSH and GPX levels were significantly restored to normal levels while LPO was significantly reduced at 200 mg/kg b. wt. Treated animals. Histopathological studies showed complete cartilage regeneration and near normal joint in ETBE treated arthritic rats. CONCLUSION: ETBE demonstrated potent anti-inflammatory activity by modulating the expression of inflammatory cytokines and restoring the antioxidant enzyme levels.


Assuntos
Albizzia , Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Artrite Experimental/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/toxicidade , Antioxidantes/farmacologia , Antioxidantes/toxicidade , Artrite Experimental/sangue , Artrite Experimental/genética , Artrite Experimental/patologia , Compostos de Bifenilo/química , Citocinas/sangue , Citocinas/genética , Etanol/química , Feminino , Glutationa/sangue , Glutationa Peroxidase/sangue , Articulações/efeitos dos fármacos , Articulações/patologia , Peroxidação de Lipídeos/efeitos dos fármacos , Óxido Nítrico/química , Estresse Oxidativo/efeitos dos fármacos , Picratos/química , Casca de Planta/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/toxicidade , Ratos Sprague-Dawley , Solventes/química , Superóxido Dismutase/sangue
15.
Funct Integr Genomics ; 20(2): 191-200, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31444657

RESUMO

Exposure to high altitude above 3000 m leads to two outcomes-acclimation or high-altitude maladies. To reach a particular outcome, the plasma proteome is modified differentially, either in context of an acclimation response or mal-acclimation response leading to disease. This ensures that hypoxia-responsive plasma protein trends reflect acclimation in acclimated individuals when compared with their levels prior to acclimation. Such protein trends could be used to assess acclimation in an individual and any significant deviation from this trend may indicate non-acclimation, thereby preventing high-altitude illnesses before they manifest. In this study, we investigate and statistically evaluate the trendlines of various hypoxia-responsive plasma protein levels, reported significantly perturbed in our previous studies, in individuals (male; n = 20) exposed to 3520 m at high-altitude day 1 (HAD1), HAD4, and HAD7L and to 4420 m at HAD7H, HAD30, and HAD120. We observe that thioredoxin (Trx), glutathione peroxidase 3 (GPx-3), and apolipoprotein AI (Apo-AI) are statistically robust markers to assess acclimation across the exposure duration while sulfotransferase 1A1 (ST1A1) is a capable negative control whose levels increase only in cases of HAPE. We also observe exposure day-specific and resident altitude-specific proteins capable of accurately assessing acclimation when compared with baseline levels or the lower altitude zone.


Assuntos
Aclimatação , Altitude , Proteínas Sanguíneas/análise , Hipóxia/sangue , Adulto , Apolipoproteína A-I/sangue , Arilsulfotransferase/sangue , Biomarcadores/sangue , Ensaio de Imunoadsorção Enzimática , Glutationa Peroxidase/sangue , Humanos , Masculino , Militares , Curva ROC , Tiorredoxinas/sangue , Fatores de Tempo , Adulto Jovem
16.
J Surg Res ; 245: 344-353, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31425874

RESUMO

BACKGROUND: Penehyclidine hydrochloride (PHC), a novel anticholinergic reagent, has been shown to exert anti-endoplasmic reticulum stress (ERS), antioxidant, and antiinflammation functions in various rat models. However, the definite pathogenesis of lung defensive roles of PHC remains unclear. This study measured the functions of PHC on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. METHODS: In this research, the LPS-induced ALI model was assessed through the branchial injection of LPS for 24 h. Male Sprague-Dawley rats were randomly allocated into 5 groups: sham, LPS, LPS + PHC (0.5 mg/kg), LPS + PHC (1 mg/kg), and LPS + PHC (2.5 mg/kg). The concentrations of superoxide dismutase, malondialdehyde, myeloperoxidase, and glutathione peroxidase were measured by enzyme-linked immunosorbent assay and immunohistochemistry analysis. Western blotting, real-time PCR, and immunofluorescence analysis were used to determine the ERS-associated protein levels and mRNA expression. The protein levels of Bax, Bcl-2, caspase-3, and caspase-9 were used to measure lung tissue apoptosis. RESULTS: The results revealed that PHC administration inhibited LPS-induced ALI as indicated by the loss in the ratio of injury production evaluated through hematoxylin-eosin staining, in particular the lung sample sections, compared with the LPS group. PHC administration inhibited LPS-induced lung myeloperoxidase and serum concentrations of malondialdehyde, superoxide dismutase, and glutathione peroxidase in rats. PHC administration repressed the LPS-activated ERS-correlated pathway and apoptosis-associated protein levels in rats. CONCLUSIONS: In summary, our findings indicated that PHC has a defensive effect on LPS-induced ALI by inhibiting oxidative stress, attenuating PERK and ATF6 signals, and suppressing ERS-mediated apoptosis.


Assuntos
Lesão Pulmonar Aguda/prevenção & controle , Apoptose/efeitos dos fármacos , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Quinuclidinas/uso terapêutico , Lesão Pulmonar Aguda/sangue , Animais , Avaliação Pré-Clínica de Medicamentos , Glutationa Peroxidase/sangue , Lipopolissacarídeos , Pulmão/metabolismo , Masculino , Malondialdeído/sangue , Peroxidase/metabolismo , Quinuclidinas/farmacologia , Distribuição Aleatória , Ratos Sprague-Dawley , Superóxido Dismutase/sangue
17.
Kurume Med J ; 65(4): 137-144, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31391380

RESUMO

A number of antioxidants have been used to treat peripheral nerve injury. However, there are few definitive experimental studies of ozone therapy for peripheral nerve cut injury. We aimed to examine the effects of mild level ozone therapy on sciatic nerve regeneration. One hundred adult male Wistar albino rats were randomly divided into four groups: group 1 (n=20) no cut injury or therapy; group 2 (n=20) sham; group 3 (n=30) nerve cut injury, no therapy; group 4 (n=30) nerve cut injury and ozone therapy. Sciatic functional index (SFI) and withdrawal reflex (WDR) were measured for all groups before nerve cut, at postoperative day 1, and at weeks 2, 4, 6 and 8. More myelinated (M) nerve fibers were observed after nerve cut injury in the ozone-therapy group. Significant differences were seen in plasma SOD (superoxide dismutase), CAT (catalase) and GPx (glutathione peroxidase) activities (p<0.05), and significant functional improvement was observed at postoperative weeks 2 and 4 (p<0.05) after ozone treatment. This is the first study conducted for the purpose of examining the effects of ozone therapy on sciatic nerve cut injury.


Assuntos
Regeneração Nervosa/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Ozônio/farmacologia , Traumatismos dos Nervos Periféricos/tratamento farmacológico , Nervo Isquiático/efeitos dos fármacos , Neuropatia Ciática/tratamento farmacológico , Animais , Catalase/sangue , Modelos Animais de Doenças , Glutationa Peroxidase/sangue , Masculino , Atividade Motora , Limiar da Dor , Traumatismos dos Nervos Periféricos/sangue , Traumatismos dos Nervos Periféricos/fisiopatologia , Ratos Wistar , Recuperação de Função Fisiológica , Nervo Isquiático/lesões , Nervo Isquiático/metabolismo , Nervo Isquiático/fisiopatologia , Neuropatia Ciática/sangue , Neuropatia Ciática/fisiopatologia , Superóxido Dismutase/sangue
18.
Life Sci ; 248: 116481, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31102744

RESUMO

AIMS: Hypobaric hypoxia (HH), linked to oxidative stress, impairs cardiac function. We synthesized a novel nitronyl nitroxide radical, an HPN derivative (HEPN) and investigated the protective effects of HEPN and HPN against HH-induced heart injury in mice and the underlying mechanisms of action. MAIN METHODS: Mice were administered with HPN (200 mg/kg) or HEPN (200 mg/kg) 30 min before exposed to HH. The cardiac function was measured. Serum AST, CK, LDH and cTnI were estimated. Heart tissue oxidase activity, SOD, CAT, GSH-Px, ROS and MDA were estimated. ATP content, Na+/K+-ATPase and Ca2+/Mg2+-ATPase activity was measured. The expression of HIF-1, VEGF, Nrf2, HO-1, Bax, Bcl-2, Caspase-3 was estimated. KEY FINDINGS: Results showed that pretreatment with HEPN or HPN led to a dramatic decrease in the activity of biochemical markers AST, CK, LDH and cTnI in murine serum. They increased the activity of SOD, CAT and GSH-Px and reduced the level of ROS and MDA in the hearts of mice. HEPN and HPN could increase the expression of Nrf2 and OH-1. They could maintain the ATPase activity. The Bax and Caspase-3 expression as well as the ratio of Bax/Bcl-2 were significantly downregulated and the Bcl-2 expression was upregulated by HPN or HEPN compared to the HH group. They may attenuate the HH-induced oxidant stress via free radical scavenging activity. SIGNIFICANCE: The present study showed that the nitronyl nitroxide radical HEPN and HPN may be potential therapeutic agents for treatment of HH-induced cardiac dysfunction.


Assuntos
Antioxidantes/farmacologia , Cardiotônicos/farmacologia , Insuficiência Cardíaca/tratamento farmacológico , Hipóxia/tratamento farmacológico , Óxidos de Nitrogênio/farmacologia , Animais , Antioxidantes/síntese química , Aspartato Aminotransferases/sangue , Aspartato Aminotransferases/genética , ATPase de Ca(2+) e Mg(2+)/genética , ATPase de Ca(2+) e Mg(2+)/metabolismo , Cardiotônicos/síntese química , Caseína Quinases/sangue , Caseína Quinases/genética , Caspase 3/genética , Caspase 3/metabolismo , Catalase/sangue , Catalase/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/sangue , Glutationa Peroxidase/genética , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/genética , Insuficiência Cardíaca/fisiopatologia , Testes de Função Cardíaca , Heme Oxigenase-1/genética , Heme Oxigenase-1/metabolismo , Hipóxia/complicações , Hipóxia/genética , Hipóxia/fisiopatologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , L-Lactato Desidrogenase/sangue , L-Lactato Desidrogenase/genética , Masculino , Malondialdeído/antagonistas & inibidores , Malondialdeído/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo , Óxidos de Nitrogênio/síntese química , Estresse Oxidativo/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/antagonistas & inibidores , Espécies Reativas de Oxigênio/metabolismo , ATPase Trocadora de Sódio-Potássio/genética , ATPase Trocadora de Sódio-Potássio/metabolismo , Superóxido Dismutase/sangue , Superóxido Dismutase/genética , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo
19.
Environ Geochem Health ; 42(2): 617-624, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31435865

RESUMO

Glutathione Peroxidase activity in whole blood is well correlated with the Selenium (Se) levels in cattle hence can be used effectively to assess the supply of Se to farm animals. In this study, Se status of cattle from five different geographic regions of Sri Lanka were assessed based on glutathione peroxidase (GSH-Px) activity. The GSH-Px activity was determined in whole blood samples collected from 80 cattle from 31 different farms in five districts viz. Kandy, Anuradhapura, Batticoloa, Trincomalee and Jaffna using photometric method. Mean GSH-Px activity was found to be 825, 1239, 1039, 849 and 1307 µkat L-1 in above districts, respectively while the reference value was considered as 665.4 µkat L-1. Among the studied animals, insufficient Se levels were detected in 50%, 17%, 9%, 27% and 5%, respectively, from above districts. Kruskal Wallis test indicated a significant variation among the sampled locations with respect to the GSH-Px activity (p = 0.001). Selenium content in pasture and water collected from studied locations varied from 6.0 to 554 µg kg-1 and < 0.03-1.14 µg L-1, respectively. The lower Se levels in feeds recorded from Kandy region infer the lower GSH-Px activity in the animals from the same region. This variability may be due to differences in nutrient supply, age and species of cattle, and lactation stage. Although the assessing method has some limitations, the activity of GSH-Px of the samples indirectly confirms that considerable numbers of cattle from Sri Lanka are with insufficient selenium levels.


Assuntos
Glutationa Peroxidase/sangue , Selênio/sangue , Ração Animal/análise , Animais , Disponibilidade Biológica , Bovinos , Feminino , Água Doce/análise , Água Subterrânea/análise , Masculino , Selênio/análise , Selênio/farmacocinética , Sri Lanka
20.
Transl Psychiatry ; 9(1): 340, 2019 12 19.
Artigo em Inglês | MEDLINE | ID: mdl-31852887

RESUMO

Telomere attrition is a hallmark of cellular aging and shorter telomeres have been reported in mood and anxiety disorders. Telomere shortening is counteracted by the enzyme telomerase and cellular protection is also provided by the antioxidant enzyme glutathione peroxidase (GPx). Here, telomerase, GPx, and telomeres were investigated in 46 social anxiety disorder (SAD) patients in a within-subject design with repeated measures before and after cognitive behavioral therapy. Treatment outcome was assessed by the Liebowitz Social Anxiety Scale (self-report), administered three times before treatment to control for time and regression artifacts, and posttreatment. Venipunctures were performed twice before treatment, separated by 9 weeks, and once posttreatment. Telomerase activity and telomere length were measured in peripheral blood mononuclear cells and GPx activity in plasma. All patients contributed with complete data. Results showed that social anxiety symptom severity was significantly reduced from pretreatment to posttreatment (Cohen's d = 1.46). There were no significant alterations in telomeres or cellular protection markers before treatment onset. Telomere length and telomerase activity did not change significantly after treatment, but an increase in telomerase over treatment was associated with reduced social anxiety. Also, lower pretreatment telomerase activity predicted subsequent symptom improvement. GPx activity increased significantly during treatment, and increases were significantly associated with symptom improvement. The relationships between symptom improvement and putative protective enzymes remained significant also after controlling for body mass index, sex, duration of SAD, smoking, concurrent psychotropic medication, and the proportion of lymphocytes to monocytes. Thus, indices of cellular protection may be involved in the therapeutic mechanisms of psychological treatment for anxiety.


Assuntos
Terapia Cognitivo-Comportamental , Glutationa Peroxidase/sangue , Avaliação de Resultados em Cuidados de Saúde , Fobia Social/sangue , Fobia Social/fisiopatologia , Fobia Social/terapia , Telomerase/sangue , Telômero/metabolismo , Adulto , Feminino , Humanos , Masculino , Índice de Gravidade de Doença , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...