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1.
Eklem Hastalik Cerrahisi ; 30(3): 282-8, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31650926

RESUMO

OBJECTIVES: This study aims to evaluate the effects of locally applied icariin on bone fracture healing in femur fractured rat model. MATERIALS AND METHODS: The study included 64 male Sprague-Dawley rats (mean age 6 months; weighing, 280-490 g) in eight main study groups. Fracture healing process and level were evaluated with radiography, histopathology and dual energy X-ray absorptiometry to investigate the effects of local administration of icariin at varying doses, which is an exogenous osteo-inductive substance. Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured in the peripheral blood in addition to glutathione (GSH) and myeloperoxidase (MPO) levels to investigate the effects of icariin on the oxidant-antioxidant systems. RESULTS: Radiological bone mineral density measurements and histopathological findings revealed that icariin improved all these parameters in the two healing periods tested. Superoxide dismutase activity decreased in association with local icariin application to the fractured side whereas GPx and GSH increased and MPO remained unchanged. Icariin increased the GPx and GSH levels which are responsible from scavenging hydroxyl radical and hydrogen peroxide. CONCLUSION: Locally administered icariin to the fracture accelerated bone healing by reducing the oxidative stress.


Assuntos
Densidade Óssea/efeitos dos fármacos , Flavonoides/farmacologia , Consolidação da Fratura/efeitos dos fármacos , Depuradores de Radicais Livres/farmacologia , Absorciometria de Fóton , Animais , Relação Dose-Resposta a Droga , Fraturas do Fêmur/diagnóstico por imagem , Fraturas do Fêmur/cirurgia , Flavonoides/administração & dosagem , Flavonoides/sangue , Depuradores de Radicais Livres/administração & dosagem , Depuradores de Radicais Livres/sangue , Glutationa Peroxidase/sangue , Masculino , Modelos Animais , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/sangue
2.
Invest Ophthalmol Vis Sci ; 60(13): 4462-4468, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31658354

RESUMO

Purpose: To investigate the serum changes of antioxidant/oxidant markers and the relationship between these factors and visual function in patients with retinitis pigmentosa (RP). Methods: Fifty-two RP patients <40 years old and 25 controls were included. Serum samples were analyzed for superoxide dismutase 3 (SOD3) activity, glutathione peroxidase (GPx), potential antioxidant (PAO), and hexanoyl-lysine (HEL). The relationships between these markers and visual parameters, including best-corrected visual acuity (BCVA), mean deviation (MD), and average retinal sensitivity of 4 or 12 central points on static perimetry tests (Humphrey Field Analyzer, the central 10-2 program) were examined in the RP patients. Results: Although there was no significant difference in the serum SOD3 activity between RP patients and controls, serum SOD3 activity in the severe degeneration group with macular involvement (16.3 ± 11.3 U/mL) was significantly lower compared with those in the mild degeneration group (those with midperipheral scotomas; 28.5 ± 16.6 U/mL, P = 0.0459). SOD3 was significantly related to visual acuity (r = -0.3701, P = 0.0069) and the average retinal sensitivity of four central points (r = 0.3463, P = 0.0137) in RP patients. The linear trends of these two parameters across SOD3 levels were also significant (P = 0.0264 and 0.0172, respectively). There was no consistent correlation between other serum antioxidant/oxidant markers and visual parameters. Conclusions: Lower serum SOD3 activity was associated with the severe retinal degeneration in RP patients. Our results suggest that serum SOD3 activity may be related to disease severity in RP.


Assuntos
Antioxidantes/metabolismo , Biomarcadores/sangue , Oxidantes/sangue , Retinite Pigmentosa/sangue , Acuidade Visual/fisiologia , Adulto , Feminino , Glutationa Peroxidase/sangue , Humanos , Lisina/sangue , Masculino , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/sangue , Retinite Pigmentosa/fisiopatologia , Superóxido Dismutase/sangue , Testes de Campo Visual , Campos Visuais/fisiologia , Adulto Jovem
3.
Diabetes Metab Syndr ; 13(4): 2541-2547, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31405674

RESUMO

AIM: Oxidative stress (OS) plays a major role in pathogenic mechanisms associated with metabolic syndrome (Mets) yet the main component of Mets contributing most to OS is not well elucidated. Hence, the aim of this study was to investigate the oxidative-antioxidative status in Mets subjects and to determine the main predicting component of OS. METHODS: Anthropometric measures, fasting blood glucose, lipid profile, antioxidant enzymes [catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx)], reduced glutathione (GSH), malondialdehyde (MDA) and protein carbonyl were assessed in 172 adult UAE residents. International Diabetes Federation criteria were used for Mets diagnosis. Mets Scores (0-5) were calculated and assigned per subject based on number of components. RESULTS: Of all participants, 22.1% had Mets and 49.4% had large waist circumference (WC). Significant lower levels of catalase, SOD, GPx and GSH, and higher levels of MDA and protein carbonyl were observed in subjects with Mets. In addition, catalase, SOD, GPx, and GSH correlated negatively, while MDA and protein carbonyl correlated positively with almost all Mets components. Similar trend of correlations was noticed with Mets Scores. When adjusted for age and gender, linear regression analysis revealed that subjects with large WC demonstrated significantly lower levels of antioxidative enzymes and GSH, and higher levels of MDA and protein carbonyl. Consequently, WC emerged as the best predictor of OS. CONCLUSIONS: The degree of OS is dependent on the Mets Scores, and WC contributes independently to increased OS among adults in UAE.


Assuntos
Biomarcadores/análise , Índice de Massa Corporal , Síndrome Metabólica/sangue , Síndrome Metabólica/epidemiologia , Estresse Oxidativo , Circunferência da Cintura , Adulto , Antioxidantes/metabolismo , Pressão Sanguínea , Estudos de Casos e Controles , Estudos Transversais , Feminino , Seguimentos , Glutationa Peroxidase/sangue , Humanos , Incidência , Masculino , Malondialdeído/sangue , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Prognóstico , Superóxido Dismutase/sangue , Emirados Árabes Unidos/epidemiologia , Adulto Jovem
4.
J Therm Biol ; 84: 451-459, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31466786

RESUMO

We aimed to determine whether the use of injectable vitamins and minerals improves growth performance and immune and antioxidant responses in dairy calves during pre- and post-weaning period in summer. Twenty dairy calves (45 days of age) were randomized to two groups (10 each): control group (CON) and treated group [TREAT; injection providing 0.20, 0.80, 0.20, 0.10, 35 and 1 mg/kg of copper, zinc, manganese selenium, and vitamins A and E, during two periods (15 days pre- and 15 days post-weaning)]. The animals were weighed and blood samples were collected on days 1, 15, 30 and 45 of the study. Levels of serum copper, selenium, zinc, and manganese were measured on day 1; and the results showed that calves were not deficient in these minerals. The TREAT group had greater BW gain during the final third of the experiment. There was an increase in total leukocyte numbers as a result of elevation in neutrophil counts (day 45) and monocytes (days 30 and 45) in the TREAT group. This group also had lower reactive oxygen species (ROS) content (days 15, 30 and 45) and lipid peroxidation (LPO; days 15 and 45). Furthermore, the TREAT group had greater antioxidant capacity against peroxyl radicals (ACAP; days 15 and 30), activities of the enzymes glutathione peroxidase (GPx; days 15, 30 and 45) and superoxide dismutase (SOD; day 15), concentrations of total serum proteins (day 30), serum globulin (days 15 and 30), ceruloplasmin (day 15), tumor necrosis factor-alpha (TNF-α), interleukin-1, (IL-1; days 30 and 45) and interferon gamma (IFNγ; day 45), compared to CON group. High respiratory rates during hot times of the day in all study calves was suggestive of heat stress. Taken together, the data suggest that mineral and vitamins injections increased the growth performance and boosted the antioxidant and immunological systems of dairy calves during the diet transition period in summer.


Assuntos
Bovinos , Suplementos Nutricionais , Metais/farmacologia , Selênio/farmacologia , Vitamina A/farmacologia , Vitamina E/farmacologia , Vitaminas/farmacologia , Animais , Bovinos/sangue , Bovinos/crescimento & desenvolvimento , Bovinos/imunologia , Bovinos/metabolismo , Citocinas/sangue , Dieta/veterinária , Feminino , Glutationa Peroxidase/sangue , Resposta ao Choque Térmico/fisiologia , Metais/sangue , Espécies Reativas de Oxigênio/sangue , Taxa Respiratória , Estações do Ano , Selênio/sangue , Superóxido Dismutase/sangue
5.
Oxid Med Cell Longev ; 2019: 3276958, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31285784

RESUMO

Alpha-lipoic acid (ALA) has been used as a dietary supplement at different doses in patients with diabetes mellitus type 2 (T2DM) due to its antioxidant, anti-inflammatory, and hypoglycemic effects. However, the reports on the effects of ALA are controversial. For this reason, the purpose of the present study was to determine the effect of 600 mg/day of ALA on the markers of oxidative stress (OxS) and inflammation and RAGE in older adults with T2DM. A quasiexperimental study was carried out with a sample of 135 sedentary subjects (98 women and 37 men) with a mean age of 64 ± 1 years, who all had T2DM. The sample was divided into three groups: (i) experimental group (EG) with 50 subjects, (ii) placebo group (PG) with 50 subjects, and control group (CG) with 35 subjects. We obtained the following measurements in all subjects (pre- and posttreatment): glycosylated hemoglobin (HbA1c), receptor for advanced glycation end products (RAGE), 8-isoprostane, superoxide dismutase (SOD), glutathione peroxidase (GPx), total antioxidant status (TAS), and inflammatory (CRP, TNF-α, IL-6, IL-8, and IL-10) markers. Regarding the effect of ALA on HbA1c, a decrease was observed in the EG (baseline 8.9 ± 0.2 vs. posttreatment 8.6 ± 0.3) and the PG (baseline 8.8 ± 0.2 vs. posttreatment 8.4 ± 0.3) compared to the CG (baseline 8.8 ± 0.3 vs. six months 9.1 ± 0.3) although the difference was not statistically significant (p < 0.05). There was a statistically significant decrease (p < 0.05) in the blood concentration of 8-isoprostane in the EG and PG with respect to the CG (EG: baseline 100 ± 3 vs. posttreatment 57 ± 3, PG: baseline 106 ± 7 vs. posttreatment 77 ± 5, and CG: baseline 94 ± 10 vs. six months 107 ± 11 pg/mL). Likewise, a statistically significant decrease (p < 0.05) in the concentration of the RAGE was found in the EG (baseline 1636 ± 88 vs. posttreatment 1144 ± 68) and the PG (baseline 1506 ± 97 vs. posttreatment 1016 ± 82) compared to CG (baseline 1407 ± 112 vs. six months 1506 ± 128). A statistically significant decrease was also observed in all markers of inflammation and in the activity of SOD and GPx in the CG with respect to the EG and PG. Our findings suggest that the administration of ALA at a dose of 600 mg/day for six months has a similar effect to that of placebo on oxidative stress, inflammation, and RAGE in older adults with T2DM. Therefore, higher doses of ALA should be tried to have this effect. This trial is registered with trial registration number ISRCTN13159380.


Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Inflamação/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Receptor para Produtos Finais de Glicação Avançada/metabolismo , Ácido Tióctico/uso terapêutico , Idoso , Antioxidantes/metabolismo , Glicemia/metabolismo , Pressão Sanguínea/efeitos dos fármacos , Proteína C-Reativa/metabolismo , Cromatografia Líquida de Alta Pressão , Diabetes Mellitus Tipo 2/sangue , Ingestão de Energia/fisiologia , Feminino , Glutationa Peroxidase/sangue , Humanos , Inflamação/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Superóxido Dismutase/sangue , Ácido Tióctico/sangue
6.
Theriogenology ; 133: 97-103, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31078069

RESUMO

The role of oxidative stress in the control of female reproduction has not been fully elucidated in ruminants; however, it seems that antioxidants can make influence to the reproductive axis at different levels. Therefore, the aim of this study was to determine the relationship between antioxidant status and concentrations of trace minerals (chromium (Cr), copper (Cu), iron (Fe), and zinc (Zn)) with postpartum luteal activity and fertility in Holstein dairy cows. The cows (n = 100, a parity range of 2-5, and a body condition score (BCS) of 3.0 ±â€¯0.25, mean ±â€¯SEM) were assigned to the experiment at second week post calving. Ovarian follicular dynamics were monitored twice a week (from 3rd to 6th weeks post calving) by transrectal ultrasonography (US). Blood samples were collected twice weekly from the 3rd to the 6th weeks post calving at timed artificial insemination (TAI), and days 32 and 50 post AI to determine superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and trace mineral concentrations. There were associations between plasma concentrations of SOD, GPX, and TAC with postpartum luteal activity (PLA, P = 0.01) and ovulation (P = 0.03). Mean plasma SOD and GPX activities and TAC levels (U/mL) were greater in cows with normal luteal activity (NLA) than prolonged luteal phase (PLP) and anovulation (AO) cows, as well as in ovulated compared to AO cows (P = 0.03). Pregnant cows had greater levels of SOD, GPX, and TAC (U/mL) at TAI than non-pregnant cows (P = 0.01). Plasma Cu and Zn concentrations increased in pregnant compared to non-pregnant cows at TAI. In conclusion, antioxidant levels and Cu and Zn concentrations were associated with PLA and fertility.


Assuntos
Aborto Animal , Antioxidantes/metabolismo , Corpo Lúteo/fisiologia , Oligoelementos/sangue , Animais , Bovinos , Cromo/sangue , Cobre/sangue , Corpo Lúteo/metabolismo , Feminino , Glutationa Peroxidase/sangue , Inseminação Artificial/veterinária , Ferro/sangue , Período Pós-Parto/metabolismo , Gravidez , Superóxido Dismutase/sangue , Zinco/sangue
7.
Int J Biol Macromol ; 135: 706-716, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31129213

RESUMO

The aim of this work is to characterize the primary structure and physicochemical properties of natural polysaccharides (GLP) and degraded polysaccharides (GLPUD) from Ganoderma lucidum, and evaluate their hypolipidemic and antioxidant activities. The results of particle size distribution and scanning electron microscopy (SEM) showed that Ganoderma lucidum polysaccharides were effectively degraded by ultrasonic method. GLPUD was composed of the same monosaccharide units as GLP but with different molar ratios. Infrared spectra and NMR showed that the primary structure of polysaccharides had not been changed by ultrasonic degradation. Meanwhile, the thermal stability of polysaccharides increased after ultrasonic treatment. After administration by GLP and GLPUD four weeks, body weight, visceral index, atherosclerosis index (AI) and biochemical indicators in serum and in liver were determined. The results showed that GLPUD had stronger hypolipidemic and antioxidant activities than GLP. GLPUD was more effective than the GLP for reducing AI, total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), raising high density lipoprotein (HDL-C) (p < 0.01), reducing malondialdehyde (MDA) content, as well as increasing the glutathione peroxidase (GSH-Px) in mice serum, increasing superoxide dismutase (SOD) activity and reducing MDA content in liver (p < 0.05 or p < 0.01). In addition, the histopathological observations of mice livers showed that GLPUD could significantly improve lipid metabolism disorder in hepatocytes. Thus, GLPUD might be tested as a more effective hypolipidemic drug.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Polissacarídeos Fúngicos/química , Polissacarídeos Fúngicos/farmacologia , Ganoderma/química , Hipolipemiantes/química , Hipolipemiantes/farmacologia , Animais , Peso Corporal/efeitos dos fármacos , Fenômenos Químicos , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Glutationa Peroxidase/sangue , Glutationa Peroxidase/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Malondialdeído/sangue , Malondialdeído/metabolismo , Camundongos , Monossacarídeos/análise , Superóxido Dismutase/sangue , Superóxido Dismutase/metabolismo , Triglicerídeos/sangue , Ondas Ultrassônicas
8.
J Trace Elem Med Biol ; 54: 110-117, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109600

RESUMO

Selenium (Se) is a trace element essential for the appropriate course of vital processes in the human body. It is also a constituent of the active center of glutathione peroxidase and other antioxidant compounds which play an important role in red-ox processes. Associations between lower blood selenium concentration and obstetric complications has been reported in many studies. The aim of this study was to determine the dietary selenium intake and serum selenium content in pregnant Polish women and relate this to antioxidant status as whole blood glutathione peroxidase (GPX) activity, serum uric acid (UA) content and serum total antioxidant status (TAS) and pregnancy complications occurrence. Ninety-four pregnant women at a mean age 30.6 ± 5.4 years from the Lower Silesia region of Poland were recruited to the study, 37% of studied group had pregnancy complications. The mean reported Se intake and serum selenium content for Polish pregnant women was in the first trimester - 53.99 µg/day and 44.36 µg/l, the second trimester - 58.93 µg/day and 43.16 µg/l and the third trimester - 62.89 µg/day and 40.97 µg/l, respectively. Selenium intake below or above recommended value hadn't significant effect on GPX activity, TAS and UA levels. There were no statistical differences in selenium intake, serum selenium content, GPX activity and TAS and UA level between physiological and complicated pregnancy, but a positive correlation between Se intake and serum selenium content was observed during all period of gestation as well as in the second trimester of pregnancy between Se intake and GPX activity in group with physiological pregnancy where selenium intake was below the recommended level. Selenium intake above the recommended level was positively correlated also with serum UA level in first and second trimester of pregnancy. Despite weak, positive correlations in the first two trimesters of pregnancy between selenium supply and GPX activity and UA concentration we concluded that selenium intake does not significantly affect during pregnancy, both: markers of the antioxidant status of pregnant women and the occurrence of pregnancy complications.


Assuntos
Antioxidantes/metabolismo , Complicações na Gravidez/sangue , Selênio/sangue , Adulto , Feminino , Glutationa Peroxidase/sangue , Humanos , Gravidez , Selênio/administração & dosagem , Ácido Úrico/sangue
9.
Molecules ; 24(8)2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30999628

RESUMO

INTRODUCTION: According to a review of the literature, there is a lack of data on the mechanisms that participate in the suppression of inflammation that accompanies polycystic ovary syndrome (PCOS). Additionally, the changes in oxidative status resulting from a low-calorie diet have not been studied in a group of women with PCOS, and the oxidation and reduction processes associated with PCOS have not been explained. MATERIAL AND METHODS: The study involved 49 women who were diagnosed with PCOS according to Rotterdam's criteria, and 24 women voluntarily agreed to a three-month dietary intervention. The dietary intervention was carried out for 3 months. Glutathione peroxidase (GPx3) activity, the Ferric reducing ability of plasma, and uric acid concentration were measured spectrophotometrically both before and after the intervention. Statistical analysis was performed with the Statistica 10.0 software package, and a Pearson's correlation matrix was generated. RESULTS: A lower concentration of GPx3 was observed in women with PCOS (before the dietetic intervention began) compared with the GPx3 levels in healthy women. A relationship was shown between GPx3 levels and the concentration of prolactin, insulin on fasting, and triglycerides. After the dietary intervention, increases in uric acid and GPx3 activity were noted, as well as numerous relationships between anthropometric and biochemical parameters. The ferric reducing/antioxidant power did not change significantly. CONCLUSIONS: Inhibiting the effect of prolactin (by the level of reactive oxygen species) on the activity of GPx3 could be a starting point for the increase in antioxidative stress and the development of the inflammatory state associated with PCOS pathophysiology. Following a low-calorie diet with a lower glycemic index is proposed to silence inflammation by increasing the concentration of uric acid. During GPx3 mobilization, women with PCOS have a higher demand for selenium, and its deficiencies may contribute to disordered thyroid hormone synthesis. The three-month dietary intervention did not silence redox processes in the examined group of women.


Assuntos
Glutationa Peroxidase/sangue , Índice Glicêmico , Síndrome do Ovário Policístico/sangue , Síndrome do Ovário Policístico/dietoterapia , Ácido Úrico/sangue , Adulto , Feminino , Humanos , Inflamação/sangue , Inflamação/dietoterapia
10.
Georgian Med News ; (287): 87-90, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30958295

RESUMO

Hormonal dysfunction of the thyroid gland causes disorder of all kinds of metabolism. Both hypothyroidism, and hyperthyroidism are the causes of imbalance in prooxidant-antioxidant system with the development of pathological process named "oxidative stress". Objective - study of oxidative stress in patients who underwent thyroid gland operation depending on its functional activity and the presence of autoimmune thyroiditis. The intensity of lipid peroxidation was examined by concentration of diene conjugates and malondialdehyde, and the activity of antioxidant system by the level of catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase of red blood cells. It was established that the activity level of the lipid peroxidation system and the activity of antioxidant defense enzymes have their specific characteristics depending on the various functional states of the thyroid gland, as well as on the presence or absence of autoimmune component in the structure of thyroid pathology. All changes occurring in patients with this pathology accompanied by hormonal imbalance and autoimmune thyroiditis, lead to development of chronic nonspecific metabolic endogenous intoxication.


Assuntos
Antioxidantes/metabolismo , Glutationa Peroxidase/sangue , Hipotireoidismo , Peroxidação de Lipídeos , Malondialdeído/sangue , Estresse Oxidativo , Glândula Tireoide/cirurgia , Catalase , Glutationa Peroxidase/metabolismo , Glutationa Redutase , Humanos , Malondialdeído/metabolismo , Superóxido Dismutase/sangue , Superóxido Dismutase/metabolismo
11.
J Anim Sci ; 97(6): 2515-2523, 2019 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31004130

RESUMO

The aim of this study was to determine the effects of dietary grape seed polyphenols (GSP) supplementation during the late gestation and lactation period on reproductive performance, antioxidative status in serum, nutrient composition, and Ig content in colostrum of multiparous sows. On day 80 of gestation, a total of 64 sows with similar body condition were allocated to a completely randomized block design with 4 dietary treatments (n = 16 sows per treatment): 1) basal diet (CON, control group); 2) basal diet supplemented with 200 IU/kg vitamin E (200VE, positive control group); 3) basal diet supplemented with 200 mg/kg GSP (200GSP); and 4) basal diet supplemented with 300 mg/kg GSP (300GSP). The trial lasted 56 d until the piglets were weaned on day 21 of lactation. Reproductive performance, parameters of antioxidative status, and levels of progesterone (P4) and estradiol (E2) in serum, nutrient composition, and Ig content in colostrum of sows were determined. The number of dead fetuses was reduced, and farrowing survival was significantly improved in the litters from 300GSP-fed (P < 0.05). Preweaning survivability significantly increased in the litters from sows fed 200GSP and 200VE (P < 0.05). The activity of superoxide dismutase and glutathione peroxidase (GSH-Px) in the serum was significantly increased in sows fed 200GSP and 300GSP (P < 0.05). The activity of GSH-Px in the serum also significantly increased in sows fed 200VE (P < 0.05). Sows fed 300GSP had the greatest levels of P4 and E2 in the serum, which was significantly greater than sows fed 200VE and CON (P < 0.05). No significant differences were found among treatments for the content of solids-not-fat, fat, protein, and lactose in colostrum (P > 0.05). However, sows fed GSP had greater IgM and IgG content in colostrum compared with sows fed 200VE and CON (P < 0.05). In conclusion, dietary GSP supplementation during late gestation and lactation improved the farrowing survival and preweaning survivability, enhanced the antioxidant status and hormone levels in serum, and increased the IgM and IgG content in colostrum of sows.


Assuntos
Antioxidantes/análise , Colostro/química , Suplementos Nutricionais , Polifenóis/farmacologia , Reprodução/efeitos dos fármacos , Vitis/química , Ração Animal/análise , Animais , Líquidos Corporais/metabolismo , Dieta/veterinária , Feminino , Glutationa Peroxidase/sangue , Imunoglobulina G/análise , Lactação/efeitos dos fármacos , Paridade , Gravidez , Distribuição Aleatória , Suínos , Vitamina E/farmacologia
12.
Biomarkers ; 24(4): 394-400, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30907677

RESUMO

Purpose: Oxidative stress (OS) has been implicated in the pathogenesis of metabolic syndrome (MetS). The acute change in OS biomarkers due to exercise, known as exercise-induced OS (EIOS), is postulated to be a more appropriate marker of OS compared to spot OS measures. These studies objectives were to investigate EIOS in participants with MetS and compare the associations between EIOS, spot OS measures and MetS severity. Methods: Sixty-three participants with MetS had MetS severity assessed using the MetS Z-score. Participants undertook a cardiorespiratory fitness test ( V O2peak) to volitional exhaustion (∼8-12 minutes). Plasma OS (total F2-isoprostanes (IsoP), protein carbonyls (PCs)) and antioxidant (glutathione peroxidase (GPx), total antioxidant status (TAS)) biomarkers were measured from samples obtained before and five minutes post- V O2peak test. Wilcoxon's signed-rank tests were used to determine changes in OS markers. Results: There were no significant (p > 0.05) changes in OS or antioxidant biomarkers from pre- to post-exercise (median (interquartile range): IsoP -15.5 (-71.8 to 47.8) pg/mL; PC -0.01 (-0.16 to 0.13) nmol/mg protein; GPx 0.76 (-4.94 to 9.82) U/L, TAS 0.03 (0.00-0.05) mmol/L). Conclusions: A V O2peak test to exhaustion failed to induce OS in participants with MetS. There were no associations between MetS severity and spot OS or EIOS biomarkers.


Assuntos
Aptidão Cardiorrespiratória , Glutationa Peroxidase/sangue , Síndrome Metabólica/sangue , Síndrome Metabólica/diagnóstico , Esforço Físico , Idoso , Biomarcadores/sangue , Glicemia/metabolismo , Estudos Transversais , Teste de Esforço/métodos , F2-Isoprostanos/sangue , Feminino , Hemoglobina A Glicada/metabolismo , Humanos , Resistência à Insulina , Masculino , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Estresse Oxidativo , Carbonilação Proteica , Índice de Gravidade de Doença
13.
Molecules ; 24(6)2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30897831

RESUMO

The mechanism of hepatoprotective compounds is usually related to its antioxidant or anti-inflammatory effects. Black garlic is produced from garlic by heat treatment and its anti-inflammatory activity has been previously reported. Therefore, the aim of this study was to investigate the hepatoprotective effect of five different extracts of black garlic against carbon tetrachloride (CCl4)-induced acute hepatic injury (AHI). In this study, mice in the control, CCl4, silymarin, and black garlic groups were orally administered distilled water, silymarin, and different fraction extracts of black garlic, respectively, after CCl4 was injected intraperitoneally to induce AHI. The results revealed that the n-butanol layer extract (BA) and water layer extract (WS) demonstrated a hepatoprotective effect by reducing the levels of alanine aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and hepatic malondialdehyde (MDA). Furthermore, the BA and WS fractions of black garlic extract increased the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rd), tumor necrosis factor alpha (TNF-α), and the interleukin-1 (IL-1ß) level in liver. It was concluded that black garlic exhibited significant protective effects on CCl4-induced acute hepatic injury.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Alho/química , Fígado/efeitos dos fármacos , Fígado/metabolismo , Extratos Vegetais/uso terapêutico , Lesão Pulmonar Aguda/sangue , Lesão Pulmonar Aguda/metabolismo , Alanina Transaminase/sangue , Fosfatase Alcalina/sangue , Animais , Antioxidantes/metabolismo , Tetracloreto de Carbono/toxicidade , Fermentação/fisiologia , Glutationa Peroxidase/sangue , Glutationa Redutase/sangue , Masculino , Malondialdeído/sangue , Extratos Vegetais/química , Ratos , Silimarina , Superóxido Dismutase/sangue , Fator de Necrose Tumoral alfa/sangue
14.
Niger J Clin Pract ; 22(3): 375-379, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30837426

RESUMO

Objective: The aim of this study was to investigate the effect of oxidative stress and antioxidant situation on chronic otitis media with effusions (COME) and acute otitis media (AOM) in children. Methods: A total of 107 children aged 2 to 13 years were examined. The study included 31 patients with AOM, 39 with COME, and 37 as control subjects. Venous blood samples were collected from all patients and control group. Myeloperoxidase (MPO), glutathione peroxidase (GPx), catalase (CAT), nitric oxide (NO), malondialdehyde (MDA), and superoxide dismutase (SOD) activities were investigated in the blood samples. Results: The mean age was found as 7.3 ± 3.3 in the AOM group, 6.2 ± 3.0 in the COME group, and 6 ± 2.4 in the control group. MPO, NO, and CAT were found to be significantly higher in the AOM and COME groups than the control groups (P = 0.040, P = 0.001, and P = 0.044). Conclusion: In this study, we observed activity of antioxidant and oxidative stress in children with COME and AOM. These results may be important in the diagnosis of these diseases and may affect the theurapeutic approach to the patients with COME and AOM.


Assuntos
Antioxidantes/metabolismo , Otite Média com Derrame/sangue , Otite Média/sangue , Estresse Oxidativo , Doença Aguda , Adolescente , Catalase/sangue , Criança , Pré-Escolar , Doença Crônica , Feminino , Glutationa Peroxidase/sangue , Humanos , Masculino , Malondialdeído/sangue , Óxido Nítrico/sangue , Peroxidase/sangue , Superóxido Dismutase/sangue
15.
Microb Pathog ; 130: 65-70, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30831228

RESUMO

This study evaluated the seropositivity of Brucella abortus and Leptospira interrogans in ewes with reproductive disturbances in southern Brazil and verified the creatine kinase (CK) activity and oxidation status via assessment of superoxide dismutase, glutathione peroxidase and glutathione transferase in serum of seropositive animals for L. interrogans serovar Icterohaemorrhagiae. For Leptospira infection 381 animals with clinical history of reproductive disturbance from Planalto Serrano de Santa Catarina (Brazil) were analyzed, showing an occurrence for L. interrogans of 20.2% from which 81.8% were seropositive for L. interrogans Icterohaemorrhagiae. Serovars Wolfii, Grippothyphosa, Bratislava, Canicola and Butembo were also identified. In the case of B. abortus, positive cases were identified by buffered acidified antigen, finding 14 positive samples, but none of them were positive after a second test (2-mercaptoethanol), showing the absence of relationship between infection with B. abortus and abortion in the tested individuals. Serum reactive oxygen species (ROS) levels and CK activity were found higher in animals positive for Leptospira infection, presenting higher titrations (1:320) than non-infected individuals. Serum glutathione peroxidase activity was higher in positive animals with titrations 1:160 and 1:320, while serum glutathione S-transferase was higher in positive individuals only for titrations 1:320. Serum superoxide dismutase showed lower activity in infected animals with titrations of 1:320. Our results show the region of Planalto Serrano de Santa Catarina with a high occurrence levels of sheep infected by L. interrogans serovar Icterohaemorrhagiae, from which animals with high titrations (1:320) present oxidative stress elicited by excessive ROS production, triggering the stimulation of antioxidant systems to counter this excess. In summary, ovine with higher titrations (1:320) present oxidative damage that can contribute to disease pathophysiology.


Assuntos
Doenças dos Genitais Femininos/veterinária , Leptospira interrogans serovar icterohaemorrhagiae/crescimento & desenvolvimento , Leptospirose/veterinária , Estresse Oxidativo , Doenças dos Ovinos/patologia , Animais , Brasil , Brucelose/complicações , Brucelose/veterinária , Creatina Quinase/sangue , Feminino , Doenças dos Genitais Femininos/patologia , Glutationa Peroxidase/sangue , Glutationa Transferase/sangue , Leptospira interrogans serovar icterohaemorrhagiae/classificação , Leptospirose/complicações , Leptospirose/patologia , Espécies Reativas de Oxigênio/sangue , Sorotipagem , Ovinos , Superóxido Dismutase/sangue
16.
J Therm Biol ; 80: 141-149, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30784478

RESUMO

The aim of this study was to evaluate whether the addition of grape pomace flour (GPF) in the diet of laying hens at the end of the productive cycle and on heat stress could exert benefits on their health and performance, as well as egg quality. For this, 74-week-old laying hens (n = 64) were divided into four groups with four repetitions each, as follow: T0 (the control group; without GPF), T1 (1% GPF), T2 (2% GPF) and T3 (3% GPF) during 35 days. Percentage of laid eggs was higher in the group T1 compared to T0, and the feed intake was higher in the groups T1, T2 and T3 compared to T0. There was no difference regarding the chemical-physical composition of fresh eggs; however, eggs from GPF-fed chickens showed changes after storage regarding specific gravity, yolk index, pH of yolk, albumen and Haugh unit compared to T0. Fresh or stored egg yolk from GPF groups showed higher antioxidant capacity and lower lipid peroxidation compared to T0. GPF (3%) prevented the reduction of monounsaturated fatty acids in the yolk of stored eggs compared to T0. Glutathione peroxidase and superoxide dismutase activities, as well as total antioxidant capacity against peroxyl radicals were higher in the serum of laying hens that received GPF compared to T0, while lipid peroxidation was lower. In summary, the addition of GPF in the diet for laying hens at the end of the productive cycle can be beneficial for animal health and exerted positive effects in their performance and egg quality.


Assuntos
Dieta/veterinária , Ovos/análise , Resposta ao Choque Térmico/efeitos dos fármacos , Preparações de Plantas/farmacologia , Vitis , Ração Animal , Animais , Galinhas , Ácidos Graxos , Feminino , Glutationa Peroxidase/sangue , Concentração de Íons de Hidrogênio , Peroxidação de Lipídeos , Soroglobulinas/análise , Superóxido Dismutase/sangue , Substâncias Reativas com Ácido Tiobarbitúrico/análise
17.
Biol Trace Elem Res ; 191(2): 354-362, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30725267

RESUMO

Selenium (Se) is an essential micronutrient required by the human body and exerts biological functions through selenoproteins. The relationship between Se and diabetes remains ambiguous. This study aimed at, through measurements of serum selenoprotein P (SelP), glutathione peroxidase (GPx3), and Se content in serum and hair, evaluating Se levels in community dwellers with type 2 diabetes mellitus (T2DM). A total of 336 subjects, including 176 T2DM patients (65.5 ± 8.7 years old) and 160 healthy residents (63.7 ± 9.8 years old, as controls), were recruited in this cross-sectional community-based study performed in Suzhou. Samples of fasting venous blood and hair were collected for measurements of Se levels, glycometabolism, and biochemistry parameters. We found that the serum Se level and SelP concentration in T2DM patients were substantially higher than those in healthy residents (p < 0.05). There was no significant difference in GPx3 activity and hair Se level between the two groups of subjects (p > 0.05). Serum Se was positively correlated with both GPx3 and hair Se in T2DM patients (r = 0.167 and 0.164, respectively, p < 0.05) and negatively correlated with SelP in healthy controls (r = - 0.293, p < 0.05). In conclusion, this study showed significantly higher levels of serum Se and SelP in community dwellers with T2DM than in matched healthy residents.


Assuntos
Diabetes Mellitus Tipo 2/sangue , Glutationa Peroxidase/sangue , Selênio/sangue , Selenoproteína P/sangue , Idoso , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Selênio/análise
18.
Cell Mol Biol (Noisy-le-grand) ; 65(1): 65-72, 2019 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-30782297

RESUMO

This study was designed to investigate the effect of dietary boron on spatial learning, anxiety, some vitamins and oxidative parameters in rats. Thirty-two Wistar albino male rats were used in the study. The rats were equally divided into four groups with 8 rats each: I control group: standard pellet diet only, II. group: 250 ppm boric acid, III. group: 500 ppm boric acid and IV. group: 1000 ppm boric acid added into standard pellet diet. Over a five-week period, elevated plus-maze test was used for anxiety assessment and Morris water maze test was used for evaluating spatial learning. Additionally, blood samples were obtained at the end of the experiment and were used to determine the serum levels of some vitamins and oxidative parameters. Dietary boron significantly increased weight gain (p<0.001) and food consumption in the 250 ppm and 500 ppm groups (p<0.05). Although boron supplementation had no significant effect on learning and anxiety-related behavior, it had beneficiary effects on memory retention in the 1000 ppm group (p<0.05). Biochemical analyses showed a significant decrease in the MDA levels (p<0.05) and an increase in vitamin D3 levels (p<0.01) in the 500 ppm group, a significant increase in GSH-Px activity in the 250 ppm and 500 ppm groups (p<0.05), and a decrease in vitamin E levels in all the experimental groups (p<0.05). In conclusion, our study demonstrated that dietary boron can be beneficial for health when administered at appropriate doses.


Assuntos
Comportamento Animal/efeitos dos fármacos , Ácidos Bóricos/farmacologia , Boro/farmacologia , Dieta , Aprendizagem/efeitos dos fármacos , Animais , Antioxidantes/metabolismo , Glutationa Peroxidase/sangue , Masculino , Malondialdeído/sangue , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ratos Wistar , Fatores de Tempo , Vitamina A/sangue , Vitamina D/sangue , Vitamina E/sangue
19.
Pak J Pharm Sci ; 32(1): 75-79, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30772793

RESUMO

Iron deficiency anemia (IDA) during pregnancy, although associated with disturbances of hematological parameters, is now also considered as a source of oxidative stress (OS). Present study aims to detect any alteration in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzymes activity in pregnant women with IDA. Levels of GSH-Px and SOD were measured in 156 anemic, pregnant women and compared with similar levels in 20 non anemic, pregnant women. Activity of SOD was found to be reduced in the anemic group when compared with the control group. We found a non- significant increase in GSH-Px activities in the anemic group. These findings could be explained in terms of OS under hypoxic condition which preserves the activity of GSH-Px with a decrease activity of SOD. A positive association was seen between IDA during pregnancy and OS with results suggesting that, apart from the deficiency of iron, some other factors are also associated for the increased OS seen during pregnancy.


Assuntos
Anemia Ferropriva/enzimologia , Glutationa Peroxidase/sangue , Estresse Oxidativo , Complicações Hematológicas na Gravidez/enzimologia , Superóxido Dismutase/sangue , Anemia Ferropriva/sangue , Anemia Ferropriva/diagnóstico , Biomarcadores/sangue , Estudos de Casos e Controles , Feminino , Humanos , Gravidez , Complicações Hematológicas na Gravidez/sangue , Complicações Hematológicas na Gravidez/diagnóstico
20.
Poult Sci ; 98(7): 2781-2789, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30778562

RESUMO

This work aimed to investigate the effects of the palygorskite (PAL) composites on the growth performance and antioxidant status in broiler chickens. A total of 192 one-day-old Ross 308 broilers were randomly divided into 3 treatment groups. Broilers were fed basal diets supplemented with either 50 mg/kg chlortetracycline (CTC group), 1 g/kg ZnO/PAL (ZnO/PAL group), or 1 g/kg chitooligosaccharides/ZnO/PAL (COS/ZnO/PAL group), respectively. The results showed that PAL composites were found to exhibit similar effects on growth performance as CTC (P > 0.05). ZnO/PAL and COS/ZnO/PAL enhanced the activity of serum glutathione peroxidase (GSH-Px) compared with CTC both at 21 and 42 d (P < 0.05). Compared with the CTC group, COS/ZnO/PAL enhanced serum catalase (CAT) activity at 21 d (P < 0.05), and decreased serum malondialdehyde (MDA) content at 42 d (P < 0.05). Compared with the CTC group, ZnO/PAL decreased duodenal mucous MDA content at 21 d, while ZnO/PAL did not affect activities of superoxide dismutase (SOD) and GSH-Px in the duodenum (P > 0.05). The duodenal mucous activities of SOD and GSH-Px were the highest in the COS/ZnO/PAL group at 42 d (P < 0.05). At 21 d, broilers in the COS/ZnO/PAL group had the lowest MDA content and the highest total antioxidant capacity (T-AOC) in the jejunum (P < 0.05). Palygorskite composites decreased ileum mucous MDA content compared with CTC treated broilers at 21 d (P < 0.05). At 42 d, ileum mucous T-AOC was increased both in the ZnO/PAL and COS/ZnO/PAL groups compared with the CTC group (P < 0.05). The ileum mucous GSH-Px activities both in the ZnO/PAL and COS/ZnO/PAL groups were increased compared with the CTC group (P < 0.05). In conclusion, the broilers given the basal diet supplemented with the PAL composites exhibited similar growth performance to their counterparts in the AGP group. Additionally, the PAL composites improved the antioxidant status of broilers and the beneficial effects of COS/ZnO/PAL on the antioxidant status are more pronounced.


Assuntos
Ração Animal/análise , Antioxidantes/metabolismo , Galinhas/crescimento & desenvolvimento , Compostos de Magnésio/farmacologia , Compostos de Silício/farmacologia , Animais , Catalase/sangue , Quitina/administração & dosagem , Quitina/análogos & derivados , Quitina/farmacologia , Clortetraciclina/administração & dosagem , Clortetraciclina/farmacologia , Dieta/veterinária , Mucosa Gástrica/metabolismo , Glutationa Peroxidase/sangue , Compostos de Magnésio/administração & dosagem , Masculino , Malondialdeído/sangue , Compostos de Silício/administração & dosagem , Superóxido Dismutase/análise , Óxido de Zinco/administração & dosagem , Óxido de Zinco/farmacologia
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