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1.
Food Chem ; 307: 125558, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31644977

RESUMO

In this work, steam explosion (SE) was exploited as a green and facile process to deconstruct liquorice's structure and deglycosylate glycyrrhizic acid (GL) to improve conversion and diffusion efficacy of GL and its hydrolyzed products. Results showed SE induced auto-hydrolysis of GL into glycyrrhetic acid 3-O-mono-ß-D-glucuronide (GAMG) and glycyrrhetinic acid (GA), by which 30.71% of GL conversion, 5.24% and 21.47% of GAMG and GA formation were obtained. GL hydrolytic pathways were revealed by reaction kinetics and thermodynamics, which possessed complex consecutive and parallel reactions with endothermic, non-spontaneous and entropy-decreasing features. SE referred to cause cleavage of the ß-1,3 glycosidic bond in GL which was hydrolyzed to GA as a main product and GAMG and glucuronic acids as minor products. Diffusion of hydrolyzed products was accelerated by raising the diffusion coefficient and shortening the equilibrium time by over 90%. This work provides a sustainable and efficient route for product conversion and function enhancement of bioactive components.


Assuntos
Glycyrrhiza/química , Ácido Glicirrízico/metabolismo , Vapor , Ácido Glicirretínico/metabolismo , Temperatura Alta , Hidrólise , Cinética , Termodinâmica
2.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3435-3440, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602906

RESUMO

The aim of this paper was to investigate the anti-inflammatory effect of Tripterygium wilfordii processed with licorice on DSS-induced ulcerative colitis( UC) mice and its regulation on intestinal immune system. In this study,a DSS-induced animal model of UC mice was established,with mesalazine( Mes) as a positive drug. The pharmacodynamic effects of low( PT1) and high( PT2)doses of T. wilfordii processed with licorice were analyzed by disease activity index( DAI),colon length and colon histopathological score in mice. By detecting the expression levels of TNF-α and IL-6 cytokines in the serum of mice,immunohistochemical CD3+T and Fox P3+Treg staining in the colon of mice,the anti-inflammatory and immunoregulatory effects of T. wilfordii processed with licorice on UC mice were analyzed. The hepatotoxicity of each dose of T. wilfordii processed with licorice was also analyzed by HE staining in liver tissue of mice and ALT and AST levels in serum. The results showed that the colitis symptoms of the mice in the PT1 group and the PT2 group were alleviated,the inflammatory cell infiltration was reduced. And the expression of inflammatory factors was decreased,the difference was statistically significant compared with the model group( P<0. 05). The HE staining and ALT and AST levels in the high dose group and low dose group were not significantly different from those in the normal group. The results showed that T. wilfordii processed with licorice has the anti-inflammatory and immunomodulatory effects on UC mice,and the dose did not show significant hepatotoxicity.


Assuntos
Anti-Inflamatórios/farmacologia , Colite Ulcerativa/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Glycyrrhiza/química , Tripterygium/química , Animais , Sulfato de Dextrana , Camundongos , Extratos Vegetais/farmacologia
3.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3454-3459, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602909

RESUMO

The present study was aimed to explore the dose-toxicity-effect relationship of Tripterygium wilfordii Hook f( TW) processed by liquorice,to establish the safe and effective therapeutic window,and further to provide scientific reference for the clinical use of TW. The toxicity and anti-inflammatory effect of six doses of raw TW and TW processed by liquorice( 0. 78,1. 56,3. 12,6. 24,12. 48,15. 60 g·kg-1) in 1-fluoro-2,4-dinitrobenzene( DNFB)-induced allergic contact dermatitis( ACD) model were mainly examined by histopathology and serum biochemistry. The liver biochemical parameters including ALT and AST,related inflammatory factors including TNF-α and IL-2,together with liver index,kidney index and the other pharmacodynamic indicators,were examined and compared. The results showed that compared with the control group,the serum levels of TNF-α and IL-2 of the model group were significantly increased( P<0. 01),which proved that the ACD model was successful. The comprehensive analysis of liver biochemical indexes,serum inflammatory factors and the other indexes showed that the safe and effective therapeutic window of TW processed by liquorice was 3. 12-12. 48 g·kg-1. The results showed the therapeutic window of TW processed by liquorice was much broader than that of raw TW. And it could provide scientific reference for the clinical rational use of TW.


Assuntos
Dermatite Alérgica de Contato/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Glycyrrhiza/química , Extratos Vegetais/farmacologia , Tripterygium/química , Animais , Citocinas/sangue
4.
Zhongguo Zhong Yao Za Zhi ; 44(16): 3512-3519, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602916

RESUMO

The aim of this paper was to systematically evaluate the toxicity-reducing effect of Tripterygium-licorice in animal experiments,and also to provide evidence for basic research on the toxicity reduction of Tripterygium wilfordii. The PubMed,EMbase,Web of Science,CBM,CNKI and Wan Fang Databases from their establishment to August 31 th,2018 were searched. Two independent reviewers screened the papers,extracted the data,assessed the risk of bias using SYRCLE assessment tool and conducted Meta-analysis with Rev Man 5. 3 software. A total of 10 papers involving 31 studies were finally included,15 studies of which were used for Meta-analysis. Four studies were included for chronic hepatotoxicity animal model. In experimental group( 34 animals),Tripterygium was administered at dose of 0. 09-0. 1 mg·kg-1·d-1,and glycyrrhizic acid was administered at dose of 90-100 mg·kg-1,both for 2 weeks; in control group( 34 animals),glycyrrhizic acid was replaced with equal volume of normal saline. Eleven studies were included for acute hepatotoxicity animal model. In experimental group( 66 animals),glycyrrhizic acid was administered at dose of 75-480 mg·kg-1 for 7 days,then glycyrrhizic acid was stopped,and Tripterygium began to be administered at dose of 0. 6-1. 0 mg·kg-1 per 24 h or 48 h for a total of 1-2 times; in control group( 66 animals),glycyrrhizic acid was replaced with equal volume of normal saline or corresponding solvent. The results of Meta-analysis showed that in both chronic hepatotoxicity animal model and acute hepatotoxicity animal model,the transaminase levels in the experimental group were lower than those in the control group( P < 0. 05). Subgroup analysis of acute hepatotoxicity animal model showed that the transaminase levels in the experimental group were lower than those in the control group for every subgroup except " glycyrrhizic acid 75 mg·kg-1" subgroup. However,in terms of the mean difference( MD) and confidence interval( CI),there was no significant difference in transaminase decline between each subgroup. Low dose of glycyrrhizic acid( 90-100 mg·kg-1) has a toxicity-reduction effect on chronic hepatotoxicity induced by tripterygium( 0. 09-0. 10 mg·kg-1). Middle and high doses of glycyrrhizic acid( 120-480 mg·kg-1) have a toxicity-reduction effect on acute hepatotoxicity induced by tripterygium( 0. 6-1. 0 mg·kg-1),but with no significant dose-effect relationship.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Medicamentos de Ervas Chinesas/toxicidade , Ácido Glicirrízico/administração & dosagem , Tripterygium/toxicidade , Animais , Medicamentos de Ervas Chinesas/administração & dosagem , Glycyrrhiza , Tripterygium/química
5.
Chem Pharm Bull (Tokyo) ; 67(10): 1104-1115, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31582630

RESUMO

Licorice is one of the ancient and most frequently applied herbs for its diverse phytochemicals. At present, wild resources of licorice have rapidly declined with increasing demand and the proportion of cultivated products in the market is quickly growing. However, the different level in chemical composition between the wild and cultivated licorice may result in the discrepancy in quality and pharmacological activity. Therefore, an ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) method combined with multivariate statistical analysis technology was employed to explore chemical composition differences. The result showed that total 63 components were identified from licorice samples. The wild and the cultivated licorice are obviously classified into two groups according to principal component analysis (PCA). PCA and partial least squared discrimination analysis (PLS-DA) were also introduced to rapidly find 14 candidate compounds between two ecotypes of licorice. Apart from glycyrrhizin, licorice saponin J2/G2, glyasperin D and dehydroglyasperin D also could be selected as chemical markers based on t-test and variable importance in the projection (VIP) value. Our study successfully established an effective method for exploring metabolite profiling between two ecotypes of licorice and laying the foundation for distinguishing wild and cultivated licorice.


Assuntos
Glycyrrhiza/metabolismo , Extratos Vegetais/metabolismo , Cromatografia Líquida , Glycyrrhiza/química , Estrutura Molecular , Análise Multivariada , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Espectrometria de Massas em Tandem , Fatores de Tempo
6.
Meat Sci ; 158: 107921, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31465948

RESUMO

The present research studied the effect of liquorice extract (in feed and/or directly in burgers) on the shelf-life of rabbit meat. Before weaning, 28 individually caged rabbit does with their litters were divided in two dietary groups: Control, receiving a commercial diet, and Liquorice, receiving the Control diet supplemented with 6 g liquorice extract/kg (L). At 12 weeks of age, 15 fattened rabbits/treatment (one rabbit/cage) were slaughtered and their hindlegs dissected. Hindleg meat was trimmed, individually minced and divided into two parts: one of them was mixed with 0.25% (w/w) L. Storage time significantly reduced the amount of polyunsaturated fatty acids in the burger, to a different magnitude depending on the unsaturation level, experimental group and storage time. The α-tocopherol content showed higher levels during storage in the burgers from the Liquorice group. Also TBARs values showed a significant positive effect of dietary liquorice and a progressive increase at days 3 and 6 of storage.


Assuntos
Antioxidantes/administração & dosagem , Glycyrrhiza/química , Produtos da Carne/análise , Carne/análise , Extratos Vegetais/administração & dosagem , Ração Animal/análise , Animais , Dieta/veterinária , Ácidos Graxos Insaturados/análise , Armazenamento de Alimentos , Coelhos
7.
Medicine (Baltimore) ; 98(35): e16601, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464895

RESUMO

BACKGROUND: Lactobacillus paracasei and Glycyrrhiza glabra have been reported as having beneficial effects on Helicobacter pylori infection. We aimed to assess the efficacy and safety of fermented milk containing L paracasei HP7 and G glabra in patients with H pylori infection. METHODS: This multicenter, prospective, randomized, double-blind, placebo-controlled clinical trial was conducted in 2 hospitals from April to December 2017. Patients with H pylori infection were randomized into either the treatment group (fermented milk with L paracasei HP7 and G glabra) or placebo group (fermented milk only) once daily for 8 weeks. The primary endpoint was the gastric load of H pylori measured by C-urea breath test (UBT). Secondary endpoints were histologic and clinical improvement. RESULTS: A total of 142 patients were randomly allocated to the treatment (n = 71) or placebo groups (n = 71). Compared to baseline data, the quantitative value of C-UBT at 8 weeks was significantly reduced in the treatment group (from 20.8 ±â€Š13.2% to 16.9 ±â€Š10.8%, P = .035), but not in the placebo group (P = .130). Chronic inflammation improved significantly only in the treatment group (P = .013), whereas the neutrophil activity deteriorated significantly only in the placebo group (P = .003). Moreover, the treatment group had significant improvement in gastrointestinal symptoms (P = .049) and quality of life (P = .029). No serious adverse events were observed. CONCLUSION: The combination of fermented milk containing L paracasei and G glabra reduced H pylori density and improved histologic inflammation. However, their mechanisms of action should be elucidated in further studies.


Assuntos
Glycyrrhiza/fisiologia , Infecções por Helicobacter/tratamento farmacológico , Lactobacillus paracasei/fisiologia , Leite/microbiologia , Probióticos/administração & dosagem , Adulto , Idoso , Animais , Testes Respiratórios , Método Duplo-Cego , Feminino , Fermentação , Infecções por Helicobacter/imunologia , Infecções por Helicobacter/psicologia , Humanos , Masculino , Pessoa de Meia-Idade , Probióticos/efeitos adversos , Estudos Prospectivos , Qualidade de Vida/psicologia , Resultado do Tratamento , Adulto Jovem
8.
J Agric Food Chem ; 67(34): 9643-9651, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31390199

RESUMO

Licorice is a traditional Chinese medicine, which is often used as sweetener and cosmetic ingredients in food and pharmaceutical industries. Among them, glycyrrhetic acid is one of the most important agents. Studies have shown that glycyrrhetic acid exhibited antitumor activities as PPARγ agonist. However, the limited number of PPARγ glycyrrhetinic agonists and their high toxicity greatly limit the design based on the structure. Therefore, clarifying the binding mode between PPARγ and small molecules, we focused on the introduction of a natural active piperazine skeleton in the position of glycyrrhetinic acid C-3. According to the Combination Principle and the Structure-Based Drug Design, 19 glycyrrhetic acid derivatives were designed and synthesized as potential PPARγ agonists. Compounds 4c and 4q were screened as high-efficiency and low-toxicity lead compounds.


Assuntos
Antineoplásicos Fitogênicos/química , Medicamentos de Ervas Chinesas/química , Ácido Glicirretínico/análogos & derivados , Glycyrrhiza/química , PPAR gama/antagonistas & inibidores , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Medicamentos de Ervas Chinesas/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Ácido Glicirretínico/química , Ácido Glicirretínico/farmacologia , Humanos , PPAR gama/metabolismo , Relação Estrutura-Atividade
9.
Molecules ; 24(16)2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31443210

RESUMO

Licorice is a popular sweetener and a thirst quencher in many food products particularly in Europe and the Middle East and also one of the oldest and most frequently used herbs in traditional Chinese medicine. As a wide application of food additive, it is necessary to clarify bioactive chemical ingredients and the mechanism of action of licorice. In this study, a network pharmacology approach that integrated drug-likeness evaluation, structural similarity analysis, target identification, network analysis, and KEGG pathway analysis was established to elucidate the potential molecular mechanism of licorice. First, we collected and evaluated structural information of 282 compounds in licorice and found 181 compounds that met oral drug rules. Then, structural similarity analysis with known ligands of targets in the ChEMBL database (similarity threshold = 0.8) was applied to the initial target identification, which found 63 compounds in licorice had 86 multi-targets. Further, molecular docking was performed to study their binding modes and interactions, which screened out 49 targets. Finally, 17 enriched KEGG pathways (p < 0.01) of licorice were obtained, exhibiting a variety of biological activities. Overall, this study provided a feasible and accurate approach to explore the safe and effective application of licorice as a food additive and herb medicine.


Assuntos
Aditivos Alimentares/química , Aditivos Alimentares/farmacologia , Glycyrrhiza/química , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Relação Estrutura-Atividade
10.
Plant Physiol Biochem ; 142: 292-302, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31351320

RESUMO

Plant extracts have recently been used as exogenous adjuvants to strengthen the endogenous plant defense systems when they grow under different environmental stresses, including salinity. The study aimed at determining the effects of seed soaking using licorice root extract (LRE) on photosynthesis and antioxidant defense systems, including transcript levels of enzyme-encoding genes in pea seedling grown under 150 mM NaCl-salinity. Salt stress reduced seedling growth, photosynthesis attributes, and K+ content, and increased oxidative stress (O2•‒ and H2O2, and MDA), Na+, and Cl-, along with an increase in antioxidative defense activities compared to control. However, LRE pretreatment enhanced seedling growth, photosynthetic attributes (chlorophylls, carotenoids, Fv/Fm, Pn, Tr, and gs), ascorbate and glutathione and their redox states, proline, soluble sugars, α-TOC, and enzyme activities compared to stressed control. LRE pretreatment also upregulated transcript levels of CAT-, SOD-, APX-, GR-, DHAR-, and PrxQ-encoding genes in salt-stressed seedlings, decreasing oxidative stress and Na+ and Cl- contents and increasing K+ content and K+/Na+ ratio.


Assuntos
Antioxidantes/metabolismo , Glycyrrhiza/química , Ervilhas/efeitos dos fármacos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Ervilhas/metabolismo , Ervilhas/fisiologia , Fotossíntese/efeitos dos fármacos , Tolerância ao Sal/efeitos dos fármacos , Plântula/efeitos dos fármacos , Plântula/metabolismo
11.
J Agric Food Chem ; 67(33): 9354-9361, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31339706

RESUMO

As a popular ingredient for western and traditional Chinese medicine, the root and rhizome of Chinese licorice (Glycyrrhiza uralensis Fisch.) is often administered in the form of a decoction. The protein nanoparticles (NPs) self-assembled during the process of decoction. A major constitutive protein (GLP) was purified and determined to have a molecular weight of 28 kDa with an N-terminal sequence of NPDGL IACYC GQYCW. Over 80% of the purified GLP self-assembled into spherical NPs with diameters of 74.1 ± 0.7 nm and ζ-potential of -24.3 ± 1.7 mV when boiled in Tris-HCl buffer (pH = 7.9, 20 mM) at 100 °C for 60 min. Each nanoparticle was estimated by the SEC-MALLS approach to be composed of approximately 23 protein molecules. The NPs and GLP showed low cellular toxicity upon four types of cells including MDCK, L-02, HepG2, and Caco2 cells, while the NPs promoted proliferation of normal hepatocytes by 67%. The NPs solubilized the insoluble astragaloside IV by encapsulation. The results suggest a great potential for GLP-NPs as a promising prototype of a type of drug vehicle, a novel source of bioactive nanomaterials from herbal proteins, as well as a new mode of function with herbal components.


Assuntos
Glycyrrhiza/química , Nanopartículas/química , Extratos Vegetais/química , Proteínas de Plantas/química , Células CACO-2 , Proliferação de Células/efeitos dos fármacos , Células Hep G2 , Temperatura Alta , Humanos , Peso Molecular , Nanoestruturas/química , Extratos Vegetais/farmacologia , Proteínas de Plantas/farmacologia , Raízes de Plantas/química
12.
Phytomedicine ; 63: 153014, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31323446

RESUMO

BACKGROUND: Epidermal growth factor receptor (EGFR) gene alterations are associated with sensitization to tyrosine kinase inhibitors such as gefitinib in lung cancer. Some patients suffering from non-small cell lung cancer (NSCLC) have difficulty in treating the cancer due to resistance acquired to gefitinib with MET amplification. Therefore EGFR and MET may be attractive targets for lung cancer therapy. PURPOSE: This study aimed to investigate the anti-cancer activity of Licochalcone (LC)B extracted from Glycyrrhiza inflata, in gefitinib-sensitive or gefitinib-resistant NSCLC cells, and to define its mechanisms. STUDY DESIGN: We investigated the mechanism of action of LCB by targeting EGFR and MET in human NSCLC cells. METHODS: We used the HCC827 and HCC827GR lines as gefitinib-sensitive and -resistant cells respectively, and determined the effects of LCB on both, by performing cell proliferation assay, flow cytometry analysis and Western blotting. Targets of LCB were identified by pull-down/kinase assay and molecular docking simulation. RESULTS: LCB inhibited both EGFR and MET kinase activity by directly binding to their ATP-binding pockets. The ability of this interaction was verified by computational docking and molecular dynamics simulations. LCB suppressed viability and colony formation of both HCC827 and HCC827GR cells while exhibiting no cytotoxicity to normal cells. The induction of G2/M cell-cycle arrest and apoptosis by LCB was confirmed by Annexin V/7-AAD double staining, ER stress and reactive oxygen species induction, mitochondrial membrane potential loss and caspase activation as well as related-proteins regulation. Inhibition of EGFR and MET by LCB decreased ERBB3 and AKT axis activation. CONCLUSION: We provide insights into the LCB-mediated mechanisms involved in reducing cell proliferation and inducing apoptosis in NSCLC cells. This occurs through dual inhibition of EGFR and MET in NSCLC cells regardless of their sensitivity or resistance to gefitinib. LCB may be a promising novel therapeutic medicine for gefitinib-sensitive or resistant NSCLC treatment.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Chalconas/farmacologia , Neoplasias Pulmonares/tratamento farmacológico , Proteínas Proto-Oncogênicas c-met/metabolismo , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Chalconas/química , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Receptores ErbB/química , Receptores ErbB/metabolismo , Gefitinibe/farmacologia , Glycyrrhiza/química , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Simulação de Acoplamento Molecular , Terapia de Alvo Molecular , Espécies Reativas de Oxigênio/metabolismo
13.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1739-1743, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342695

RESUMO

Grade of Chinese medicine slices is the most clear and most direct way to characterize the quality of Chinese medicine slices,also the basis of realizing " good quality and good prices",and it can guarantee the industry health development. Therefore,grade evaluation of Chinese medicine slices( GECMS) is highly valued and has grown rapidly in the industry. In recent years,due to the comprehensive and measureable features,the Chinese medicine quality constant evaluation method has been gradually recognized and applied.The paper is to establish a method of grades evaluation of Glycyrrizae Radix et Rhizoma Praeparata Cum Melle( GRRPCM) based on the Chinese medicine quality constant. 20 batches of samples were collected from Chinese herbal slices enterprises and 14 batches of qualified samples were selected to determine their external morphological indexes and inner quality indexes,then their Chinese medicine quality constants were calculated and the grades were determined. The results revealed that the relative quality constant of these samples ranged from 0. 70 to 14. 08,with a percentage quality constant from 4. 95 to 100. 00. If these samples were divided into three grades: the relative quality constant shall be ≥11. 27 or percentage quality constant ≥80. 03 for the first grade; the relative quality constant shall be <11. 27 but ≥7. 04,or percentage quality constant <80. 03 and ≥49. 99 for the second grade; while for the third grade,the relative quality constant shall be <7. 04 or the percentage quality constant <49. 99. This research indicates that Chinese quality constant can be used to objectively grade the herbal slices,providing reference for grades standard development of complex processing slices. In addition,the connotation of GECMS that has evaluate the mass discrepancy is discussed for expanding application.


Assuntos
Medicamentos de Ervas Chinesas/normas , Glycyrrhiza/química , Medicina Tradicional Chinesa , Raízes de Plantas/química , Controle de Qualidade , Rizoma/química
14.
Zhongguo Zhong Yao Za Zhi ; 44(9): 1755-1759, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31342698

RESUMO

Quality constant is a kind of grading evaluation method of traditional Chinese medicine pieces based on the combination of traditional knowledge and modern quality control. This method has been successfully applied in the grading evaluation of Glycyrrizae Radix et Rhizome pieces and traditional Chinese medicine pieces,and with a positive influence in the industry. With Dachuanxiong Formula for example,different grades of Ligusticum chuanxiong and Gastrodia elata pieces formed high-quality,moderate and qualified Dachuanxiong Formula on the basis of the grading evaluation of the pieces,and the pharmacodynamics method was used to evaluate its efficacy. The results showed that the maximum vascular diastolic rates of Dachuanxiong Formula in the three grades were( 80. 3±5. 2) %,( 67. 0±6. 1) %and( 60. 3±6. 5) %,and the strength of pharmacodynamics was positively correlated with the grade of L. chuanxiong and G. elata pieces.The quality constant technique can objectively and quantitatively classify single decoctions,and has important correlations and prompts for the efficacy of traditional Chinese medicine prescriptions composed of these pieces,with important significance in promoting hierarchical management of the industry,implementing better price for high quality and defining high quality and superior effect.


Assuntos
Medicamentos de Ervas Chinesas/normas , Gastrodia/química , Glycyrrhiza/química , Ligusticum/química , Medicina Tradicional Chinesa , Raízes de Plantas/química , Controle de Qualidade , Rizoma/química
15.
J Pharm Biomed Anal ; 175: 112715, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31336287

RESUMO

A systematic quantitative fingerprint method (SQFM) was applied to evaluate the quality of compound liquorice tablets (CPLTs) in this paper. The method contained three main parameters: macroscopic qualitative similarity (Sm), macro quantitative similarity (Pm) and a leveling variance coefficient (α), which were used to analyze the similarity of curves and evaluate the fingerprints of CPLTs. Firstly, Differential Scanning Calorimetry (DSC) was applied to analyze CPLTs and the active raw materials (RMs). At the same time, the change of appearance and weight during DSC testing revealed that the two main thermal processes of CPLTs were between 240-320 °C and 400-500 °C. The DSC fingerprint (DSC-FP) of 49 batches of CPLTs was collected and the enthalpy values were calculated. Then, we studied the dissolution of CPLTs, collected ultraviolet fingerprints (UV-FP) and the Pm was used to plot the dissolution curve. The results showed that the dissolution and enthalpy had a negative correlation, and the formula was y= -32.38x+3207.49 with r= -0.83. Finally, the High Performance Liquid Chromatography fingerprint (HPLC-FP) of 49 batches of CPLTs was collected and assessed by SQFM. The results of quality evaluation of CPLTs by HPLC-FP combined with DSC-FP showed that 49 batches were divided into 4 levels, which could evaluate the quality of the drug more comprehensively and objectively.


Assuntos
Glycyrrhiza/química , Comprimidos/química , Varredura Diferencial de Calorimetria/métodos , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Medicina Tradicional Chinesa/métodos , Controle de Qualidade , Solubilidade
16.
Curr Gastroenterol Rep ; 21(8): 39, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31289950

RESUMO

PURPOSE OF REVIEW: Popular remedies are of ongoing interest to patients experiencing common esophageal symptoms, particularly as typical pharmacologic interventions have been subject to increased scrutiny. Herein we summarize the available data regarding potential risks and benefits of several such remedies. RECENT FINDINGS: With emphasis on reflux and non-cardiac chest pain, research is ongoing into the clinical utility and diverse physiologic mechanisms underlying a variety of complementary and alternative modalities, including dietary manipulation, apple cider vinegar, melatonin, acupuncture, and various herbal products (rikkunshito, STW 5, slippery elm, licorice, and peppermint oil, among others). A substantial gap persists between anecdotal and empirical understandings of the majority of non-pharmacologic remedies for esophageal symptoms. This landscape of popular treatments nevertheless raises several interesting mechanistic hypotheses and compelling opportunities for future research.


Assuntos
Terapias Complementares/métodos , Refluxo Gastroesofágico/terapia , Ácido Acético , Terapia por Acupuntura/métodos , Dor no Peito/terapia , Medicamentos de Ervas Chinesas/uso terapêutico , Refluxo Gastroesofágico/dietoterapia , Glycyrrhiza , Humanos , Melatonina/uso terapêutico , Extratos Vegetais/uso terapêutico , Óleos Vegetais/uso terapêutico , Ulmus
17.
Spec Care Dentist ; 39(5): 505-514, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31287184

RESUMO

PURPOSE: To compare the effect of educational (sign language and video modeling) and therapeutic intervention (liquorice) on oral hygiene status of children with hearing impairment (CHI). METHODS: Ninety-three CHI, in the age range of 5-15 years, were recruited in to the study. Children were assigned to two educational intervention groups: sign language and video modeling. Each group was again randomly divided into two: with one subgroup receiving therapeutic intervention using liquorice as a mouth wash and the other group receiving no intervention. For all children, baseline oral hygiene, gingival, and plaque index scores were recorded and oral prophylaxis was performed. Based on the subgroup to which the child was assigned, oral hygiene instructions were given on a weekly basis, whereas therapeutic intervention was performed twice daily for 28 days. Reassessment was done after the completion of interventions and after 3 months, followed by statistical analysis. RESULTS: There was a significant mean reduction in oral hygiene, gingival, and plaque scores in all the children. The educational intervention could not influence the scores recorded, but the therapeutic intervention with liquorice led to a reduction in all the oral health parameters during the follow-up periods. CONCLUSION: Therapeutic intervention using liquorice as mouth wash along with educational intervention can be suggested in CHI.


Assuntos
Glycyrrhiza , Perda Auditiva , Adolescente , Criança , Pré-Escolar , Humanos , Antissépticos Bucais , Saúde Bucal , Línguas de Sinais
18.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2131-2138, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355572

RESUMO

The dose-toxicity-effect relationship between licorice combined with rhubarb in purgation was studied. A total of 108 ICR mice were divided into control group,model group,positive group,low,medium and high-dose rhubarb groups,and low,medium and high-dose rhubarb-liquorice decoction group. After 6 days of continuous administration of loperamide hydrochloride,the constipation model of mice was replicated,and each group was given lactulose,different doses of rhubarb and rhubarb-liquorice decoction for 14 days. After administration,the defecation characteristics,blood biochemistry,liver,kidney and colon pathological changes in each group were compared. Based on the objective weight given by factor analysis,the dose-toxicity-effect relationship was comprehensively analyzed by multi-index scoring method. Two common factors were extracted by factor analysis,representing effect and toxicity respectively. The results showed that rhubarb could exert a diarrhea effect at the dosage of 1/2,2 and 8 times of the high limit set forth in the Chinese Pharmacopoeia,increase the defecation volume and the intestinal tract propulsion rate,reduce the time of anal and the oral transmission,and increase the water content of feces. The combination with licorice could alleviate its diarrhea effect,especially at the dosage of 1/2 times of the high limit set forth in the Chinese Pharmacopoeia. However,rhubarb showed obvious hepatic and colon toxicities at the dosage of 2 and 8 times of the high limit set forth in the Chinese Pharmacopoeia,and the combination with licorice could significantly reduce its toxicity. It shows that licorice has a " mediating" effect on rhubarb by alleviating the purgation property and reducing the toxicity.


Assuntos
Catárticos/farmacologia , Glycyrrhiza/química , Extratos Vegetais/farmacologia , Rheum/química , Animais , Colo , Relação Dose-Resposta a Droga , Rim , Fígado , Camundongos , Camundongos Endogâmicos ICR , Testes de Toxicidade
19.
Microbiol Immunol ; 63(7): 251-260, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31166029

RESUMO

Streptococcus mutans is a cariogenic bacterium that localizes in the oral cavity. Glycyrrhetinic acid (GRA) is a major component of licorice extract. GRA and several derivatives, including disodium succinoyl glycyrrhetinate (GR-SU), are known to have anti-inflammatory effects in humans. In this study, the antimicrobial effect of GRA and its derivatives against the S. mutans UA159 strain were investigated. Minimum inhibitory concentrations (MICs) of GRA and GR-SU showed antibacterial activity against the S. mutans strain, whereas other tested derivatives did not. Because GR-SU is more soluble than GRA, GR-SU was used for further experiments. The antibacterial activity of GR-SU against 100 S. mutans strains was evaluated and it was found that all strains are susceptible to GR-SU, with MIC values below 256 µg/mL. A cell viability assay showed that GR-SU has a bacteriostatic effect on S. mutans cells. As to growth kinetics, sub-MICs of GR-SU inhibited growth. The effect of GR-SU on S. mutans virulence was then investigated. GR-SU at sub-MICs suppresses biofilm formation. Additionally, GR-SU greatly suppresses the pH drop caused by the addition of glucose and glucose-induced expression of the genes responsible for acid production (ldh and pykF) and tolerance (aguD and atpD). Additionally, expression of enolase, which is responsible for the carbohydrate phosphotransferase system, was not increased in the presence of GR-SU, indicating that GR-SU suppresses incorporation of sugars into S. mutans. In conclusion, GR-SU has antibacterial activity against S. mutans and also decreases S. mutans virulence.


Assuntos
Antibacterianos/farmacologia , Ácido Glicirretínico/farmacologia , Glycyrrhiza/química , Extratos Vegetais/farmacologia , Streptococcus mutans/efeitos dos fármacos , Antibacterianos/química , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Genes Bacterianos/genética , Glucose/metabolismo , Ácido Glicirretínico/química , Concentração de Íons de Hidrogênio , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Extratos Vegetais/química , Streptococcus mutans/genética , Streptococcus mutans/crescimento & desenvolvimento , Virulência , Fatores de Virulência/genética , Fatores de Virulência/metabolismo
20.
Chem Pharm Bull (Tokyo) ; 67(6): 534-539, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31155558

RESUMO

One triterpene and five triterpene glycosides, including four new compounds, have been identified in the underground parts of Glycyrrhiza bucharica, which was shown to be closely related to Glycyrrhizin-producing Glycyrrhiza species, G. uralensis, G. glabra and G. inflata, based on their chloroplast rbcL sequences. Two known compounds were identified squasapogenol and macedonoside C. The structures of four new compounds, bucharosides A, B, C, and D, were determined to be 3-O-α-L-rhamnopyranosyl-(1→2)-ß-D-glucuronopyranosyl-(1→2)-ß-D-glucuronopyranosyl-22-O-α-L-rhamnopyranosyl squasapogenol, 3-O-α-L-rhamnopyranosyl-(1→2)-ß-D-glucuronopyranosyl-(1→2)-ß-D-glucuronopyranosyl-macedonic acid, 3-O-α-L-rhamnopyranosyl-(1→2)-ß-D-glucuronopyranosyl-(1→2)-ß-D-glucuronopyranosyl-squasapogenol, and 22-O-α-L-rhamnopyranosyl squasapogenol, respectively. Contents of these triterpene glycosides were less than 0.5% of dry weight, and no main saponin, like glycyrrhizin or macedonoside C found in other Glycyrrhiza species, was found in the underground parts of G. bucharica.


Assuntos
Glycyrrhiza/química , Extratos Vegetais/química , Glicosídeos/química , Glicosídeos/isolamento & purificação , Glycyrrhiza/metabolismo , Espectroscopia de Ressonância Magnética , Conformação Molecular , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Tadjiquistão , Terpenos/química , Terpenos/isolamento & purificação , Triterpenos/química , Triterpenos/isolamento & purificação
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