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1.
PLoS One ; 15(8): e0231805, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32760118

RESUMO

Boron (B) is required during all growth stages of cotton crop, especially during boll formation. However, Typic Haplocambid soils of cotton growing belt in Pakistan are B-deficient, which results in low yield and economic returns. Foliar application of B improves cotton productivity; however, information is limited on the role of soil applied B in improving cotton growth and yield. The current study investigated the role of soil applied B in improving growth, yield and fiber quality of cotton crop. Five different B doses (i.e., 0.00, 2.60, 5.52, 7.78 and 10.04 mg kg-1 of soil) and two cotton cultivars (i.e., CIM-600 and CIM-616) were included in the study. Soil applied B (2.60 mg kg-1) significantly improved growth, yield, physiological parameters and fiber quality, while 10.04 mg kg-1 application improved B distribution in roots, seeds, leaves and stalks. Significant improvement was noted in plant height (12%), leaf area (3%), number of bolls (48%), boll size (59%), boll weight (52%), seed cotton yield (52%), photosynthesis (50%), transpiration rate (10%), stomatal conductance (37%) and water use efficiency (44%) of CIM-600 with 2.60 mg kg-1 compared to control treatment of CIM-616. Similarly, B accumulation in roots, seeds, leaves and stalk of CIM-600 was improved by 76, 41, 86 and 70%, respectively compared to control treatment. The application of 2.60 mg kg-1 significantly improved ginning out turn (6%), staple length (3.5%), fiber fineness (17%) and fiber strength (5%) than no B application. The results indicated that cultivar CIM-600 had higher ginning out turn (1.5%), staple length (5.4%), fiber fineness (15.5%) and fiber strength (1.8%) than CIM-616. In crux, 2.60 mg kg-1 soil B application improved growth, yield, physiological and fiber quality traits of cotton cultivar CIM-600. Therefore, cultivar CIM-600 and 2.60 mg kg-1 soil B application is recommended for higher yield and productivity.


Assuntos
Boro/metabolismo , Gossypium/crescimento & desenvolvimento , Gossypium/metabolismo , Biomassa , Fibra de Algodão/análise , Fertilizantes , Paquistão , Fotossíntese , Folhas de Planta/química , Raízes de Plantas/química , Sementes/química , Solo/química , Água
2.
Plant Mol Biol ; 104(1-2): 67-79, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32621165

RESUMO

Acetylation and deacetylation of histones are important for regulating a series of biological processes in plants. Histone deacetylases (HDACs) control the histone deacetylation that plays an important role in plant response to abiotic stress. In our study, we show the evidence that GhHDT4D (a member of the HD2 subfamily of HDACs) is involved in cotton (Gossypium hirsutum) response to drought stress. Overexpression of GhHDT4D in Arabidopsis increased plant tolerance to drought, whereas silencing GhHDT4D in cotton resulted in plant sensitivity to drought. Simultaneously, the H3K9 acetylation level was altered in the GhHDT4D silenced cotton, compared with the controls. Further study revealed that GhHDT4D suppressed the transcription of GhWRKY33, which plays a negative role in cotton defense to drought, by reducing its H3K9 acetylation level. The expressions of the stress-related genes, such as GhDREB2A, GhDREB2C, GhSOS2, GhRD20-1, GhRD20-2 and GhRD29A, were significantly decreased in the GhHDT4D silenced cotton, but increased in the GhWRKY33 silenced cotton. Given these data together, our findings suggested that GhHDT4D may enhance drought tolerance by suppressing the expression of GhWRKY33, thereby activating the downstream drought response genes in cotton.


Assuntos
Secas , Gossypium/metabolismo , Histona Desacetilases/metabolismo , Estresse Fisiológico/fisiologia , Acetilação , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Inativação Gênica , Gossypium/genética , Histona Desacetilases/genética , Histonas/genética , Histonas/metabolismo , Filogenia , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Estresse Fisiológico/genética , Transcriptoma
3.
Mol Genet Genomics ; 295(6): 1393-1400, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32651630

RESUMO

Anthocyanins are a group of important secondary metabolites, functioning as colorant in plant organs as well as protective agents against several stresses. Sub-red plant (Rs) cottons, accumulating moderate level of anthocyanins in shoots, had increased photosynthesis efficiency compared to green- (GL) and red-plant (R1) cottons. The present work aimed to clarify the molecular base of anthocyanin regulation in Rs cotton. It was found that GhPAP1A was significantly up-regulated in Rs plants compared to GL cottons, but its expression level is lower than that of GhPAP1D in R1 plants. Virus induced gene silencing of GhPAP1s inhibited the red pigmentation in Rs plants. Comparative cloning revealed a 50-bp tandem repeat in the promoter of GhPAP1A in Rs cotton, which showed stronger activity to drive the expression of downstream genes in petals. Considered that the coding sequence of GhPAP1As from Rs and GL cottons had similar functions to promote anthocyanin biosynthesis in transgenic tobaccos, we attributed moderate anthocyanin accumulation in Rs cotton to increased transcription of GhPAP1A, resulted from varied promoter structure. Our works suggested GhPAP1s as useful tool to manipulate anthocyanin level and several breeding targets, including herbivore- and pathogen- resistance, high photosynthesis efficiency and colored fibers.


Assuntos
Antocianinas/biossíntese , Regulação da Expressão Gênica de Plantas , Gossypium/metabolismo , Pigmentação/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/metabolismo , Gossypium/genética , Gossypium/crescimento & desenvolvimento , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética
4.
PLoS One ; 15(6): e0235317, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32598401

RESUMO

The Dof (DNA-binding one zinc finger) transcription factor family is a representative of plant-specific classes of transcription factors. In this study, we performed a genome-wide screening and characterization of the Dof gene family within two tetraploid species Gossypium barbadense, Gossypium hirsutum, and two diploid species Gossypium arboreum, Gossypium raimondii. 115, 116, 55 and 56 Dof genes were identified respectively and all of the genes contain a sequence encoding the Dof DNA-binding domain. Those genes were unevenly distributed across 13/26 chromosomes of the cotton. Genome comparison revealed that segmental duplication may have played crucial roles in the expansion of the cotton Dof gene family, and tandem duplication also played a minor role. Analysis of RNA-Seq data indicated that cotton Dof gene expression levels varied across different tissues and in response to different abiotic stress. Overall, our results could provide valuable information for better understanding the evolution of cotton Dof genes, and lays a foundation for future investigation in cotton.


Assuntos
Proteínas de Ligação a DNA/genética , Diploide , Genoma de Planta , Gossypium/genética , Proteínas de Plantas/genética , Tetraploidia , Dedos de Zinco/genética , Cromossomos de Plantas/genética , Proteínas de Ligação a DNA/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Gossypium/crescimento & desenvolvimento , Gossypium/metabolismo , Família Multigênica , Filogenia , Proteínas de Plantas/metabolismo
5.
PLoS One ; 15(3): e0230519, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32187234

RESUMO

Promoters are specified segments of DNA that lead to the initiation of transcription of a specific gene. The designing of a gene cassette for plant transformation is significantly dependent upon the specificity of a promoter. Constitutive Cauliflower mosaic virus promoter, CaMV35S, due to its developmental role, is the most commonly used promoter in plant transformation. While Gossypium hirsutum (Gh) being fiber-specific promoter (GhSCFP) specifically activates transcription in seed coat and fiber associated genes. The Expansin genes are renowned for their versatile roles in plant growth. The overexpression of Expansin genes has been reported to enhance fiber length and fineness. Thus, in this study, a local Cotton variety was transformed with Expansin (CpEXPA1) gene, in the form of two separate cassettes, each with a different promoter, named as 35SEXPA1 and FSEXPA1 expressed under CaMV35S and GhSCFP promoters respectively. Integration and Spatiotemporal relative expression of the transgene were studied in an advanced generation. GhSCFP bearing transgene expression was significantly higher in Cotton fiber than other plant parts. While transgene with CaMV35S promoter was found to be continually expressing in all tissues but the expression was lower in fiber than that expressed under GhSCFP. The temporal expression profile was quite interesting with a gradual increasing pattern of both constructs from 1DPA (days post anthesis) to 18DPA and decreased expression from 24 to 30 DPA. Besides the relative expression of promoters, fiber cellulose quantification and fluorescence intensity were also observed. The study significantly compared the two most commonly used promoters and it is deduced from the results that the GhSCFP promoter could be used more efficiently in fiber when compared with CaMV35S which being constitutive in nature preferred for expression in all parts of the plant.


Assuntos
Fibra de Algodão , Gossypium/genética , Gossypium/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Regiões Promotoras Genéticas/genética
6.
Artigo em Inglês | MEDLINE | ID: mdl-32114400

RESUMO

Protein acetylation (KAC) is a significant post-translational modification, which plays an essential role in the regulation of growth and development. Unfortunately, related studies are inadequately available in angiosperms, and to date, there is no report providing insight on the role of protein acetylation in cotton fiber development. Therefore, we first compared the lysine-acetylation proteome (acetylome) of upland cotton ovules in the early fiber development stages by using wild-type as well as its fuzzless-lintless mutant to identify the role of KAC in the fiber development. A total of 1696 proteins with 2754 acetylation sites identified with the different levels of acetylation belonging to separate subcellular compartments suggesting a large number of proteins differentially acetylated in two cotton cultivars. About 80% of the sites were predicted to localize in the cytoplasm, chloroplast, and mitochondria. Seventeen significantly enriched acetylation motifs were identified. Serine and threonine and cysteine located downstream and upstream to KAC sites. KEGG pathway enrichment analysis indicated oxidative phosphorylation, fatty acid, ribosome and protein, and folate biosynthesis pathways enriched significantly. To our knowledge, this is the first report of comparative acetylome analysis to compare the wild-type as well as its fuzzless-lintless mutant acetylome data to identify the differentially acetylated proteins, which may play a significant role in cotton fiber development.


Assuntos
Fibra de Algodão , Gossypium , Óvulo Vegetal , Acetilação , Fibra de Algodão/análise , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Gossypium/genética , Gossypium/metabolismo , Mutação , Óvulo Vegetal/genética , Óvulo Vegetal/metabolismo , Proteínas de Plantas/metabolismo , Processamento de Proteína Pós-Traducional
7.
PLoS One ; 15(1): e0228241, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32004326

RESUMO

Melatonin (MT; N-acetyI-5-methoxytryptamine) is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation mechanism by which exogenous melatonin mediates salt tolerance in cotton is still largely unknown. To investigate the effect of salt stress on melatonin concentration in germinating cotton seeds, we analyzed melatonin content over time during seed germination under different treatments. Melatonin content reached its minimum at day 6, while cotton germination rates peaked at day 6, indicating melatonin content and seed germination are correlated. Then we investigated the effects of 10-100 µM melatonin treatments on membrane lipid peroxides and osmotic adjustment substances during cotton seed germination under salt stress. Salt stress led to electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H2O2), malondialdehyde (MDA), organic osmotic substances (i.e., proline, soluble sugars), and inorganic osmotic substances (i.e., Na+, Cl-). Meanwhile, the contents of melatonin, soluble proteins, and K+ as well as the K+/Na+ balance decreased, indicating that salt stress inhibited melatonin synthesis and damaged cellular membranes, seriously affecting seed germination. However, melatonin pretreatment at different concentrations alleviated the adverse effects of salt stress on cotton seeds and reduced EL as well as the contents of H2O2, MDA, Na+, and Cl-. The exogenous application of melatonin also promoted melatonin, soluble sugar, soluble proteins, proline, and K+/Na+ contents under salt stress. These results demonstrate that supplemental melatonin can effectively ameliorate the repression of cotton seed germination by enhancing osmotic regulating substances and adjusting ion homeostasis under salt stress. Thus, melatonin may potentially be used to protect cotton seeds from salt stress, with the 20 µM melatonin treatment most effectively promoting cotton seed germination and improving salt stress tolerance.


Assuntos
Germinação/efeitos dos fármacos , Gossypium/efeitos dos fármacos , Gossypium/crescimento & desenvolvimento , Melatonina/farmacologia , Osmose/efeitos dos fármacos , Estresse Salino/efeitos dos fármacos , Sementes/efeitos dos fármacos , Permeabilidade da Membrana Celular/efeitos dos fármacos , Gossypium/citologia , Gossypium/metabolismo , Malondialdeído/metabolismo , Sementes/crescimento & desenvolvimento
8.
PLoS One ; 15(2): e0228675, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049975

RESUMO

Aspergillus tubingensis is an important pathogen of economically important crops. Different biotic stresses strongly influence the balance of metabolites in plants. The aim of this study was to understand the function and response of resistance associated metabolites which, in turn are involved in many secondary metabolomics pathways to influence defense mechanism of cotton plant. Analysis of non-targeted metabolomics using ultra high performance liquid chromatography-mass spectrometry (UPLC-MS) revealed abundant accumulation of key metabolites including flavonoids, phenylpropanoids, terpenoids, fatty acids and carbohydrates, in response to leaf spot of cotton. The principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and partial least squares discriminant analysis (PLS-DA) score plots illustrated the evidences of variation between two varieties of cotton under mock and pathogen inoculated treatments. Primary metabolism was affected by the up regulation of pyruvate and malate and by the accumulation of carbohydrates like cellobiose and inulobiose. Among 241 resistance related (RR) metabolites, 18 were identified as resistance related constitutive (RRC) and 223 as resistance related induced (RRI) metabolites. Several RRI metabolites, identified in the present study were the precursors for many secondary metabolic pathways. These included phenylpropanoids (stilbenes and furanocoumarin), flavonoids (phlorizin and kaempferol), alkaloids (indolizine and acetylcorynoline) and terpenoids (azelaic acid and oleanolic acid). Our results demonstrated that secondary metabolism, primary metabolism and energy metabolism were more active in resistant cultivar, as compared to sensitive cultivar. Differential protein and fatty acid metabolism was also depicted in both cultivars. Accumulation of these defense related metabolites in resistant cotton cultivar and their suppression in susceptible cotton cultivar revealed the reason of their respective tolerance and susceptibility against A. tubingensis.


Assuntos
Aspergillus/patogenicidade , Resistência à Doença , Gossypium/metabolismo , Metaboloma , Folhas de Planta/metabolismo , Cumarínicos/metabolismo , Ácidos Graxos/metabolismo , Flavonoides/metabolismo , Gossypium/microbiologia , Folhas de Planta/microbiologia , Estilbenos/metabolismo , Terpenos/metabolismo
9.
Artigo em Inglês | MEDLINE | ID: mdl-32005380

RESUMO

The soil-born vascular disease Verticillium wilt, which is caused by fungal pathogen Verticillium dahliae, is a devastating disease of cotton worldwide. In the last decade, a large number of genes have been found to participate in cotton-V. dahliae interactions, but the detailed mechanisms of cotton resistance to V. dahliae remain unclear. Here, we functionally characterized MPK3, a MAPK gene from cotton. MPK3 was induced in the roots of both resistant and susceptible cotton cultivars by V. dahliae inoculation. Transgenic cotton and tobacco with constitutively higher GbMPK3 expression conferred higher V. dahliae susceptibility, while MPK3 knockdown in cotton has limited effect on cotton resistance to V. dahliae. Expression profiling revealed that SA-mediated defense pathway genes (WRKY70, PR1, and PR5) accumulated after V. dahliae inoculation in roots of both wild-type and transgenic cotton, and the expression levels of these genes were higher in GbMPK3-overexpressing plants than in wild-type plants, indicating that GbMPK3 upregulation may reduce plant resistance to V. dahliae through regulating salicylic acid signaling transduction.


Assuntos
Resistência à Doença/genética , Regulação da Expressão Gênica de Plantas , Gossypium/genética , Ácido Salicílico/metabolismo , Transdução de Sinais , Verticillium/fisiologia , Gossypium/metabolismo , Gossypium/microbiologia , Lycopersicon esculentum/genética , Lycopersicon esculentum/metabolismo , Lycopersicon esculentum/microbiologia , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Plantas Geneticamente Modificadas/microbiologia
10.
Artigo em Inglês | MEDLINE | ID: mdl-32050119

RESUMO

Crops, including cotton, are sensitive to nitrogen (N) and excessive use can lead to an increase in production costs and environmental problems. We hypothesized that the use of cotton genotypes with substantial root systems and high genetic potentials for nitrogen-use efficiency (NUE) would best address these problems. Therefore, the interspecific variations and traits contributing to NUE in six cotton genotypes having contrasting NUEs were studied in response to various nitrate concentrations. Large genotypic variations were observed in morphophysiological and biochemical traits, especially shoot dry weight, root traits, and N-assimilating enzyme levels. The roots of all the cotton genotypes were more sensitive to low-than high-nitrate concentrations, and the genotype CCRI-69 had the largest root system irrespective of the nitrate concentration. The root morphological traits were positively correlated with N-utilization efficiency and were more affected by genotype than nitrate concentration. Conversely, growth and N-assimilating enzyme levels were more affected by nitrate concentration and were positively correlated with N-uptake efficiency. Based on shoot dry weight, CCRI-69 and XLZ-30 were identified as N-efficient and N-inefficient genotypes, respectively, and these results were confirmed by their contrasting root systems, N metabolism, and NUEs. In the future, multi-omics techniques will be performed to identify key genes/pathways involved in N metabolism, which may have the potential to improve root architecture and increase NUE.


Assuntos
Gossypium , Nitrogênio , Raízes de Plantas , Genótipo , Gossypium/genética , Gossypium/crescimento & desenvolvimento , Gossypium/metabolismo , Nitratos/metabolismo , Nitrogênio/metabolismo , Fenótipo , Raízes de Plantas/genética , Raízes de Plantas/metabolismo
11.
J Biosci ; 452020.
Artigo em Inglês | MEDLINE | ID: mdl-32020913

RESUMO

High-yielding Indian cotton varieties are not amenable for regeneration and transformation because they are recalcitrant in nature. In this work, we have developed Narasimha (NA1325) cotton variety by introducing three Cry genes driven by three different promoters conferring insect resistance. The meristematic region of embryo axis explants were infected and co-cultivated with Agrobacterium tumefacience (LBA4404) harbouring pMDC100 vector with three Cry gene cassettes (alpha-globulin : Cry2Ab, DECaMV35s : Cry1F and nodulin : Cry1Ac) with Npt II as a selectable marker gene. Out of 1010 embryo axes explants infected, 121 (T0) regenerated under two rounds of kanamycin selectionmedium.About 2551T1 seedswere collected from111T0 plants and these seeds screened again with kanamycin at seedling stage. The transgenic plants were characterized by PCR, real time quantitative PCR, lateral flow strip protein assay and insect bioassay. Out of 145 kanamycin resistant plants (T1), twelve showed amplification of all four transgenes: Npt II, Cry2Ab, Cry1F and Cry1Ac through PCR with expected amplicons as 395, 870, 840 and 618 bp, respectively. Further, lateral flow strip test revealed Cry1F and Cry1Ac proteins accumulated in 12 plants, whereas Cry2Ab protein was detected in eight only. The transcripts of all three Cry genes were accumulated significantly higher in transgenic plants at T2 generation. The transgenic lines showed effective resistance againstHelicoverpa armigera and Spodoptera litura larvae. The T2 line L-3 exhibited highest percentage of insect mortality, in which transcripts of all cry genes were accumulated higher than other plants. The transgenic cotton plants carrying triple Cry genes could be an excellent germplasmresource for the breeders for introgressions.


Assuntos
Agrobacterium tumefaciens/metabolismo , Proteínas de Bactérias/genética , Gossypium/genética , Gossypium/metabolismo , Larva/virologia , Nucleopoliedrovírus/patogenicidade , Plantas Geneticamente Modificadas/genética , Animais , Proteínas de Insetos , Receptores de Superfície Celular , Transformação Genética , Transgenes
12.
Nat Chem Biol ; 16(3): 250-256, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31932723

RESUMO

In plants, lineage-specific metabolites can be created by activities derived from the catalytic promiscuity of ancestral proteins, although examples of recruiting detoxification systems to biosynthetic pathways are scarce. The ubiquitous glyoxalase (GLX) system scavenges the cytotoxic methylglyoxal, in which GLXI isomerizes the α-hydroxy carbonyl in the methylglyoxal-glutathione adduct for subsequent hydrolysis. We show that GLXIs across kingdoms are more promiscuous than recognized previously and can act as aromatases without cofactors. In cotton, a specialized GLXI variant, SPG, has lost its GSH-binding sites and organelle-targeting signal, and evolved to aromatize cyclic sesquiterpenes bearing α-hydroxyketones to synthesize defense compounds in the cytosol. Notably, SPG is able to transform acetylated deoxynivalenol, the prevalent mycotoxin contaminating cereals and foods. We propose that detoxification enzymes are a valuable source of new catalytic functions and SPG, a standalone enzyme catalyzing complex reactions, has potential for toxin degradation, crop engineering and design of novel aromatics.


Assuntos
Aromatase/metabolismo , Lactoilglutationa Liase/química , Lactoilglutationa Liase/metabolismo , Aromatase/química , Produtos Biológicos , Catálise , Citosol/metabolismo , Glutationa/metabolismo , Gossypium/metabolismo , Complexos Multienzimáticos , Aldeído Pirúvico/química , Aldeído Pirúvico/metabolismo
13.
Plant Mol Biol ; 102(4-5): 553-567, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31989373

RESUMO

KEY MESSAGE: Overexpression of K2-NhaD in transgenic cotton resulted in phenotypes with strong salinity and drought tolerance in greenhouse and field experiments, increased expression of stress-related genes, and improved regulation of metabolic pathways, such as the SOS pathway. Drought and salinity are major abiotic stressors which negatively impact cotton yield under field conditions. Here, a plasma membrane Na+/H+ antiporter gene, K2-NhaD, was introduced into upland cotton R15 using an Agrobacterium tumefaciens-mediated transformation system. Homozygous transgenic lines K9, K17, and K22 were identified by PCR and glyphosate-resistance. TAIL-PCR confirmed that T-DNA carrying the K2-NhaD gene in transgenic lines K9, K17 and K22 was inserted into chromosome 3, 19 and 12 of the cotton genome, respectively. Overexpression of K2-NhaD in transgenic cotton plants grown in greenhouse conditions and subjected to drought and salinity stress resulted in significantly higher relative water content, chlorophyll, soluble sugar, proline levels, and SOD, CAT, and POD activity, relative to non-transgenic plants. The expression of stress-related genes was significantly upregulated, and this resulted in improved regulation of metabolic pathways, such as the salt overly sensitive pathway. K2-NhaD transgenic plants growing under field conditions displayed strong salinity and drought tolerance, especially at high levels of soil salinity and drought. Seed cotton yields in transgenic line were significantly higher than in wild-type plants. In conclusion, the data indicate that K2-NhaD transgenic lines have great potential for the production of stress-tolerant cotton under field conditions.


Assuntos
Gossypium/metabolismo , Proteínas de Plantas/fisiologia , Tolerância ao Sal/genética , Trocadores de Sódio-Hidrogênio/fisiologia , Secas , Gossypium/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Trocadores de Sódio-Hidrogênio/genética , Trocadores de Sódio-Hidrogênio/metabolismo , Estresse Fisiológico , Água/metabolismo
14.
BMC Genomics ; 21(1): 91, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996127

RESUMO

BACKGROUND: Plant NADPH oxidase (NOX), also known as respiratory burst oxidase homolog (rboh), encoded by the rboh gene, is a key enzyme in the reactive oxygen species (ROS) metabolic network. It catalyzes the formation of the superoxide anion (O2•-), a type of ROS. In recent years, various studies had shown that members of the plant rboh gene family were involved in plant growth and developmental processes as well as in biotic and abiotic stress responses, but little is known about its functional role in upland cotton. RESULTS: In the present study, 26 putative Ghrboh genes were identified and characterized. They were phylogenetically classified into six subfamilies and distributed at different densities across 18 of the 26 chromosomes or scaffolds. Their exon-intron structures, conserved domains, synteny and collinearity, gene family evolution, regulation mediated by cis-acting elements and microRNAs (miRNAs) were predicted and analyzed. Additionally, expression profiles of Ghrboh gene family were analyzed in different tissues/organs and at different developmental stages and under different abiotic stresses, using RNA-Seq data and real-time PCR. These profiling studies indicated that the Ghrboh genes exhibited temporal and spatial specificity with respect to expression, and might play important roles in cotton development and in stress tolerance through modulating NOX-dependent ROS induction and other signaling pathways. CONCLUSIONS: This comprehensive analysis of the characteristics of the Ghrboh gene family determined features such as sequence, synteny and collinearity, phylogenetic and evolutionary relationship, expression patterns, and cis-element- and miRNA-mediated regulation of gene expression. Our results will provide valuable information to help with further gene cloning, evolutionary analysis, and biological function analysis of cotton rbohs.


Assuntos
Genoma de Planta , Genômica , Gossypium/genética , Família Multigênica , NADPH Oxidases/genética , Biologia Computacional/métodos , Evolução Molecular , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Genômica/métodos , Gossypium/metabolismo , Sequenciamento de Nucleotídeos em Larga Escala , Anotação de Sequência Molecular , Filogenia , Regiões Promotoras Genéticas , Espécies Reativas de Oxigênio/metabolismo , Sequências Reguladoras de Ácido Nucleico , Estresse Fisiológico , Sintenia
15.
Mol Biol Rep ; 47(3): 1573-1581, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31933260

RESUMO

Transcriptional factors are the major regulators of plant signaling pathways in response to environmental stresses i.e., drought, salinity and cold. Hereby, the GhMYB108-like was characterized to determine whether it regulate these stresses. The GhMYB108-like cDNA consisted of 1107 base pairs (bp) with 807 open reading frame encoded a protein of 268 amino acids. Its isoelectric point and molecular weight are 5.51 and 30.3 kDa respectively. Phylogenetic analysis and online databases revealed that GhMYB108-like proteins are closely related with the Arabidopsis thaliana MYB2. Important cis-elements were detected in the promotor region of GhMYB108-like responding to stresses and phytohormones. The 3D structure of GhMYB108-like protein has been predicted. In addition, various physico-chemical properties of GhMYB108-like have been determined. Subcellular localization confirmed that GhMYB108-like are nuclear localized protein. Quantitative expression analysis showed that polyethylene glycol and salt treatments significantly induced the expression of GhMYB108-like. Overall, our findings suggest that GhMYB108-like is an important gene that would plays important regulatory role in response to drought and salt stresses.


Assuntos
Regulação da Expressão Gênica de Plantas/genética , Gossypium/genética , Proteínas de Plantas/genética , Proteínas Proto-Oncogênicas c-myb/genética , Elementos Reguladores de Transcrição/genética , Estresse Fisiológico , Sequência de Aminoácidos , Sequência de Bases , Secas , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Gossypium/metabolismo , Filogenia , Fitocromo/farmacologia , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Proteínas Proto-Oncogênicas c-myb/classificação , Proteínas Proto-Oncogênicas c-myb/metabolismo , Salinidade , Homologia de Sequência de Aminoácidos , Cloreto de Sódio/farmacologia
16.
PLoS One ; 14(12): e0225876, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31794580

RESUMO

Begomoviruses interfere with host plant machinery to evade host defense mechanism by interacting with plant proteins. In the old world, this group of viruses are usually associated with betasatellite that induces severe disease symptoms by encoding a protein, ßC1, which is a pathogenicity determinant. Here, we show that ßC1 encoded by Cotton leaf curl Multan betasatellite (CLCuMB) requires Gossypium hirsutum calmodulin-like protein 11 (Gh-CML11) to infect cotton. First, we used the in silico approach to predict the interaction of CLCuMB-ßC1 with Gh-CML11. A number of sequence- and structure-based in-silico interaction prediction techniques suggested a strong putative binding of CLCuMB-ßC1 with Gh-CML11 in a Ca+2-dependent manner. In-silico interaction prediction was then confirmed by three different experimental approaches: The Gh-CML11 interaction was confirmed using CLCuMB-ßC1 in a yeast two hybrid system and pull down assay. These results were further validated using bimolecular fluorescence complementation system showing the interaction in cytoplasmic veins of Nicotiana benthamiana. Bioinformatics and molecular studies suggested that CLCuMB-ßC1 induces the overexpression of Gh-CML11 protein and ultimately provides calcium as a nutrient source for virus movement and transmission. This is the first comprehensive study on the interaction between CLCuMB-ßC1 and Gh-CML11 proteins which provided insights into our understating of the role of ßC1 in cotton leaf curl disease.


Assuntos
Begomovirus/metabolismo , Calmodulina , Gossypium , Doenças das Plantas , Proteínas de Plantas , Calmodulina/genética , Calmodulina/metabolismo , Gossypium/genética , Gossypium/metabolismo , Gossypium/virologia , Doenças das Plantas/genética , Doenças das Plantas/virologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Tabaco/genética , Tabaco/metabolismo , Tabaco/virologia
17.
Genes (Basel) ; 10(12)2019 12 04.
Artigo em Inglês | MEDLINE | ID: mdl-31817162

RESUMO

Chalcone isomerase (CHI) is a key component of phenylalanine metabolism that can produce a variety of flavonoids. However, little information and no systematic analysis of CHI genes is available for cotton. Here, we identified 33 CHI genes in the complete genome sequences of four cotton species (Gossypium arboretum L., Gossypium raimondii L., Gossypium hirsutum L., and Gossypium barbadense L.). Cotton CHI proteins were classified into two main groups, and whole-genome/segmental and dispersed duplication events were important in CHI gene family expansion. qRT-PCR and semiquantitative RT-PCR results suggest that CHI genes exhibit temporal and spatial variation and respond to infection with Fusarium wilt race 7. A preliminary model of CHI gene involvement in cotton evolution was established. Pairwise comparison revealed that seven CHI genes showed higher expression in cultivar 06-146 than in cultivar Xinhai 14. Overall, this whole-genome identification unlocks a new approach to the comprehensive functional analysis of the CHI gene family, which may be involved in adaptation to plant pathogen stress.


Assuntos
Fusarium/metabolismo , Regulação da Expressão Gênica de Plantas , Gossypium , Liases Intramoleculares , Família Multigênica , Doenças das Plantas , Biologia Computacional , Fusarium/genética , Regulação Enzimológica da Expressão Gênica , Estudo de Associação Genômica Ampla , Gossypium/genética , Gossypium/metabolismo , Gossypium/microbiologia , Liases Intramoleculares/biossíntese , Liases Intramoleculares/genética , Doenças das Plantas/genética , Doenças das Plantas/microbiologia
18.
Int J Mol Sci ; 20(24)2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31817342

RESUMO

Cytoplasmic male sterility (CMS) is an important agronomic feature and provides an effective tool for heterosis utilization of crops. This study reports the comparative transcriptomic sketches between a novel allohexaploid cotton progeny CMS line LD6A and its maintainer line LD6B using de novo transcriptome sequencing technology at the pollen abortion stage. A total of 128,901 Unigenes were identified, in which 2007 were upregulated and 11,864 were downregulated. The significantly differentially expressed genes (DEGs) in LD6A show a distant and diverse genetic nature due to their distant hybrid hexaploidy progeny. Further analysis revealed that most of the DEGs participated in the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, histone acetyltransferase activity, sepal development, stigma development, cotyledon development and microsporogenesis. A highly differentially expressed toxic protein, Abrin, was identified in the CMS line LD6A, which can catalyze the inactivation of ribosomes and consequently lead to cell death through the mitochondrial pathway in human cells. Twelve DEGs were selected randomly to validate transcriptome data using quantitative reverse-transcribed PCR (qRT-PCR). This study will contribute to new ideas and foundations related to the molecular mechanism of CMS and the innovation of cotton germplasm resources.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Gossypium , Infertilidade das Plantas , Proteínas de Plantas , Proteômica , Transcriptoma , Gossypium/genética , Gossypium/metabolismo , Proteínas de Plantas/biossíntese , Proteínas de Plantas/genética
19.
BMC Genomics ; 20(1): 993, 2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31856713

RESUMO

BACKGROUND: F-box proteins are substrate-recognition components of the Skp1-Rbx1-Cul1-F-box protein (SCF) ubiquitin ligases. By selectively targeting the key regulatory proteins or enzymes for ubiquitination and 26S proteasome mediated degradation, F-box proteins play diverse roles in plant growth/development and in the responses of plants to both environmental and endogenous signals. Studies of F-box proteins from the model plant Arabidopsis and from many additional plant species have demonstrated that they belong to a super gene family, and function across almost all aspects of the plant life cycle. However, systematic exploration of F-box family genes in the important fiber crop cotton (Gossypium hirsutum) has not been previously performed. The genome-wide analysis of the cotton F-box gene family is now possible thanks to the completion of several cotton genome sequencing projects. RESULTS: In current study, we first conducted a genome-wide investigation of cotton F-box family genes by reference to the published F-box protein sequences from other plant species. 592 F-box protein encoding genes were identified in the Gossypium hirsutume acc.TM-1 genome and, subsequently, we were able to present their gene structures, chromosomal locations, syntenic relationships with their parent species. In addition, duplication modes analysis showed that cotton F-box genes were distributed to 26 chromosomes, with the maximum number of genes being detected on chromosome 5. Although the WGD (whole-genome duplication) mode seems play a dominant role during cotton F-box gene expansion process, other duplication modes including TD (tandem duplication), PD (proximal duplication), and TRD (transposed duplication) also contribute significantly to the evolutionary expansion of cotton F-box genes. Collectively, these bioinformatic analysis suggest possible evolutionary forces underlying F-box gene diversification. Additionally, we also conducted analyses of gene ontology, and expression profiles in silico, allowing identification of F-box gene members potentially involved in hormone signal transduction. CONCLUSION: The results of this study provide first insights into the Gossypium hirsutum F-box gene family, which lays the foundation for future studies of functionality, particularly those involving F-box protein family members that play a role in hormone signal transduction.


Assuntos
Proteínas F-Box/genética , Gossypium/genética , Proteínas de Plantas/genética , Proteínas F-Box/classificação , Proteínas F-Box/metabolismo , Duplicação Gênica , Ontologia Genética , Genoma de Planta , Gossypium/metabolismo , Família Multigênica , Reguladores de Crescimento de Planta/fisiologia , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Proteínas Ligases SKP Culina F-Box/fisiologia , Transdução de Sinais
20.
PLoS One ; 14(12): e0226887, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31860646

RESUMO

Soil carbon (C), nitrogen (N) and phosphorus (P) are important soil properties linked to nutrient limitation and plant productivity in terrestrial ecosystems. Up to 90% of the Yellow River Delta (YRD), China has been affected by soil salination due to groundwater overdraft, improper irrigation, land use and land cover change. The objective of this study is to evaluate the impact of different plant communities on soil quality in a saline-alkaline system of the YRD. We investigated the vertical distribution and seasonal variation of soil C, N, and P, and C:N ratio by choosing four dominant plant communities, namely, alfalfa grassland (AG), Chinese tamarisk (CT), locust forest (LF) and cotton field (CF). The results showed that the concentrations of soil organic carbon (SOC) and total nitrogen (TN) in CT and LF were always higher than that in AG and CF, especially in the topsoil layer (p<0.05), then gradually decreased with soil depth increasing (p<0.05). The C:N ratio was generally lower, and the average C:N ratio was higher in LF (11.55±1.99) and CT (11.03±0.47) than in CF (10.05±1.25) and AG (9.11±1.11) (p<0.05). The available phosphorus (AP) was highest in CT in Spring, while it was highest in CF in Summer and Autumn. It is worth noting that the soil AP concentrations were always low, particularly in AG (< 6.29 mg kg-1) and LF (< 4.67 mg kg-1), probably linked to P poorly mobile in the saline-alkaline region. In this study, soil nutrients in natural plant communities are superior to farmland, and are significantly affected by the types of plant community; therefore, we suggest that protection of natural vegetation and development of optimal vegetation are critical to restoring land degradation in the YRD.


Assuntos
Carbono/análise , Florestas , Pradaria , Nitrogênio/análise , Fósforo/análise , Solo/química , Áreas Alagadas , Acacia/metabolismo , China , Gossypium/metabolismo , Medicago sativa/metabolismo , Dispersão Vegetal , Rios , Estações do Ano , Tamaricaceae/metabolismo
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