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1.
Dent Med Probl ; 56(2): 161-165, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31274254

RESUMO

BACKGROUND: Endodontic sealers are essential for sealing gutta-percha to the dentin walls. They help to ensure that the canal remains free of microorganisms which might lead to infection. In order to perform their intended function, the sealers should properly adhere to the dentin walls and remain insoluble when set in the canal. OBJECTIVES: The purpose of this study was to evaluate the bond strength and solubility of a novel polydimethylsiloxane-gutta-percha calcium silicate-containing root canal sealer (GuttaFlow® bioseal) and compare it with the zinc oxide and eugenol sealer (Zical®). MATERIAL AND METHODS: The endodontic sealers used in this study were GuttaFlow bioseal and Zical. The bond strength was assessed using push-out bond strength test in 3 root segments: coronal, middle and apical. The solubility was tested according to the American National Standards Institute / American Dental Association (ANSI/ADA) specification No. 57 at 3 different time intervals: 1, 7 and 14 days. RESULTS: The push-out bond strength in all root segments was significantly higher in Zical compared to GuttaFlow bioseal. The solubility was significantly higher on day 1 and 7 in Zical compared to GuttaFlow bioseal, and on day 14, the difference between them was not significant. CONCLUSIONS: Within the limitations of this study, the endodontic sealer GuttaFlow bioseal showed low bond strength values compared to Zical. The solubility of the set GuttaFlow bioseal and Zical were both within the recommended ANSI/ADA levels.


Assuntos
Compostos de Cálcio , Guta-Percha , Materiais Restauradores do Canal Radicular , Silicatos , Compostos de Cálcio/farmacocinética , Compostos de Cálcio/farmacologia , Cavidade Pulpar , Dimetilpolisiloxanos , Resinas Epóxi , Guta-Percha/farmacocinética , Guta-Percha/farmacologia , Materiais Restauradores do Canal Radicular/farmacocinética , Materiais Restauradores do Canal Radicular/farmacologia , Silicatos/farmacocinética , Silicatos/farmacologia , Solubilidade , Estados Unidos
2.
Photodiagnosis Photodyn Ther ; 26: 137-141, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30902793

RESUMO

INTRODUCTION: The aim of this study was to evaluate the influence of final irrigation protocols and endodontic sealer on bond strength of root filling material on root dentin previously treated with photodynamic therapy (PDT). METHODS: One hundred root canals were prepared up to #F3 file of Pro-Taper system to receive the root filling material. All samples were submitted to PDT and randomly divided into five groups (n = 20) according to final irrigation protocols: Group 1-distilled water + ultrasonic activation (US); Group 2-17% EDTA; Group 3-QMix; Group 4-17% EDTA + US; Group 5-QMix + US. Each group was randomly divided into two subgroups (n = 10), according to the endodontic sealer used for cementation of gutta-percha points: AH Plus or MTA Fillapex. The bond strength was evaluated by a push-out test. The patterns of failure were observed under optical microscopy. The bond strength was evaluated using a two-way Anova followed by the Tukey post-hoc test, and the failure mode was evaluated using the chi-square test (α = 5%). RESULTS: The use of 17% EDTA and QMix associated or not to US improved the bond strength of root filling material with either endodontic sealer (p < 0.05). AH Plus showed higher bond strength than MTA Fillapex (p < 0.05). There was a higher predominance of cohesive failure in all groups, regardless of the tested final irrigation protocols and endodontic sealer (p < 0.05). CONCLUSIONS: The use of 17% EDTA and QMix, regardless of association with US, and the use of AH Plus improve the bond strength of the root filling material on root dentin previously treated with PDT.


Assuntos
Dentina/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/farmacologia , Preparo de Canal Radicular/métodos , Biguanidas/farmacologia , Colagem Dentária , Ácido Edético/farmacologia , Resinas Epóxi/farmacologia , Guta-Percha/farmacologia , Humanos , Técnicas In Vitro , Teste de Materiais , Fotoquimioterapia , Fármacos Fotossensibilizantes , Polímeros/farmacologia , Distribuição Aleatória
3.
Odontology ; 107(1): 64-71, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29651668

RESUMO

The aim of the present study was to compare the effect of chitosan nanoparticle, QMix, and 17% EDTA on the penetrability of a calcium silicate-based sealer into dentinal tubules using a confocal laser scanning microscope (CLSM). Sixty mandibular premolar teeth were selected and randomly divided into three groups (n = 20) before root canal preparation according to the solution used in the final rinse protocol: chitosan, QMix, and EDTA groups. Twenty teeth of each group were filled with a TotalFill BC sealers' single gutta-percha cone and with 0.1% rhodamine B. The specimens were horizontally sectioned at 3 and 5 mm from the apex, and the slices were analyzed in CLSM (4×). Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy with using Image J analysis software. Dentinal tubule's penetration depth, percentage, and area were measured using imaging software. Kruskal-Wallis test was used for statistical analysis. The level of significance was set at 5%. Results of Kruskal-Wallis analysis showed that there was a significant difference in the percentage and depth of sealer penetration among all groups at 3 and 5 mm level sections (P < 0.05). Within the groups, the minimum sealer penetration depth was recorded for chitosan nanoparticle group. Greater depth of sealer penetration was recorded at 5 mm as compared to 3 mm in all the groups. Within the limitation of the present study, it can be concluded that QMix and EDTA promoted sealer penetration superior to that achieved by chitosan nanoparticle.


Assuntos
Biguanidas/farmacologia , Quitosana/farmacologia , Dentina/efeitos dos fármacos , Ácido Edético/farmacologia , Polímeros/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Dente Pré-Molar , Dentina/ultraestrutura , Guta-Percha/farmacologia , Humanos , Processamento de Imagem Assistida por Computador , Técnicas In Vitro , Microscopia Confocal , Nanopartículas , Rodaminas/farmacologia , Obturação do Canal Radicular
4.
ScientificWorldJournal ; 2018: 1920946, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30111988

RESUMO

Background: Vertical root fractures (VRFs) can significantly reduce dental prognosis. Cone-beam computed tomography (CBCT) offers better visualization of VRF than conventional radiography. However, gutta-percha creates artifacts in cone-beam CT (CBCT) images and reduces the diagnosis quality. The purpose of this study was to evaluate the accuracy of CBCT in detection of VRF in presence and absence of gutta-percha in canals. Materials and Methods: In this cross-sectional study, 50 extracted mandibular premolars were selected. After preparing the access cavity, canals were instrumented using step-back method, and gutta-percha #40 was placed afterwards. The fractures were created using electromechanical universal testing machine on 25 teeth. The teeth were randomly placed in dry mandibular dental sockets, and the first CBCTs were taken. Then the gutta-percha was removed, and the second CBCTs were taken. Results: The results of Kappa coefficient between two observers in roots with and without gutta-percha were 0.644 and 0.830, respectively (p value ≤ 0.001). The sensitivity and specificity of VRF diagnosis in assessing gutta-percha filled canals were 32% and 68% for the first observer, respectively (Kappa < 0.000, p value = 1.000), and 40% and 68% for the second observer, respectively (Kappa = 0.080, p value = 0.556). The sensitivity and specificity of VRF diagnosis in assessing the empty canals (without gutta-percha) were 72% and 96% for the first observer, respectively (Kappa = 0.680, p value ≤ 0/001), and 72% and 96% for the second observer, respectively (Kappa = 0.680, p value ≤ 0/001). Conclusion: The intracanal filling materials such as gutta-percha reduce the diagnostic ability of vertical root fractures. Hence, it is recommended to remove those materials from root canals before imaging to improve the diagnostic potential of CBCT.


Assuntos
Tomografia Computadorizada de Feixe Cônico/métodos , Guta-Percha/farmacologia , Raiz Dentária/efeitos dos fármacos , Raiz Dentária/diagnóstico por imagem , Estudos Transversais , Fraturas Ósseas/diagnóstico por imagem , Humanos , Raiz Dentária/lesões
5.
Biomed Pharmacother ; 106: 1506-1512, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30119226

RESUMO

OBJECTIVES: The aim of this study was to evaluate the subcutaneous response induced by Roeko Guttaflow2 (RG), Sealapex Xpress (SX), AH Plus (AHP) sealers. METHODS: 100 BALB/c mice received implants in the subcutaneous tissue with the tested materials (10 animals per period for each evaluated sealer) and were evaluated after different experimental periods (7, 21 and 63 days), in each animal was placed a tube, the control group was an empty tube. Histological analysis evaluated semi-quantitatively the inflammatory infiltration, collagen fiber formation and tissue thickness. In addition, immunohistochemistry was performed for interleukin-6 (IL-6). Data were statistically analyzed (α = 0.05). RESULTS: RG promoted a greater collagen fiber formation at 7 days and 63 days compared to the CG (p = 0.004) and AHP (p = 0.005) respectively, while at 21 days, the SX promoted a greater reaction (p = 0.021). For the tissue thickness, there was a greater reaction at 7 days with CG (p = 0.0156) and with RG at 63 days (p = 0.03). Regarding the inflammatory infiltrate, there was no difference at 7 days and 63 days (p = 0.5; p = 0.27), while at 21 days, a statistically difference was found between SX, CG (p = 0.04) and RG (p = 0.027). In addition, the presence of IL-6 was observed in almost all groups, with a more intense marking at 7days. SIGNIFICANCE: All cements evaluated presented a satisfactory tissue response, however, RG was the one that presented a more satisfactory tissue response.


Assuntos
Hidróxido de Cálcio/farmacologia , Dimetilpolisiloxanos/farmacologia , Resinas Epóxi/farmacologia , Guta-Percha/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Salicilatos/farmacologia , Tela Subcutânea/efeitos dos fármacos , Animais , Hidróxido de Cálcio/toxicidade , Dimetilpolisiloxanos/toxicidade , Combinação de Medicamentos , Resinas Epóxi/toxicidade , Colágenos Fibrilares/metabolismo , Migração de Corpo Estranho/induzido quimicamente , Migração de Corpo Estranho/metabolismo , Migração de Corpo Estranho/patologia , Guta-Percha/toxicidade , Mediadores da Inflamação/metabolismo , Interleucina-6/metabolismo , Masculino , Camundongos Endogâmicos BALB C , Medição de Risco , Materiais Restauradores do Canal Radicular/toxicidade , Salicilatos/toxicidade , Tela Subcutânea/metabolismo , Tela Subcutânea/patologia , Fatores de Tempo
6.
Implant Dent ; 26(6): 911-914, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29084027

RESUMO

AIM: The aim of this in vitro study was to assess the antimicrobial efficacy of materials (gutta percha [GP], polytetrafluoroethylene [PTFE] tape, and vinyl polysiloxane [VP]) used for sealing the implant abutment screw hole (IASH). METHODS: Sixty implants with a Morse cone connection were used. The implants were randomly divided into 3 groups (20 implants/group). In groups 1, 2, and 3, the IASH was sealed with GP, PTFE, and VP, respectively. Unstimulated whole saliva (UWS) was used as a contaminant medium for the incubation of the specimens. After 7 days of incubation, the specimens were opened under aseptic conditions, and the contents of the inner parts of the IASH were collected using sterile microbrushes. The occurrence of microbial leakage through the screw access channel interface was assessed with the checkerboard DNA-DNA hybridization method. P < 0.05 was considered statistically significant. RESULTS: Among samples in groups 1, 2, and 3, the UWS collected before incubation showed no statistically significant differences in relation to the prevalence of species investigated. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Candida albicans were isolated from group 1 than groups 2 and 3. CONCLUSION: The choice of material for sealing the IASH is entirely dependent on the operators' choice and experience.


Assuntos
Antibacterianos/farmacologia , Dente Suporte , Implantes Dentários/microbiologia , Guta-Percha/farmacologia , Politetrafluoretileno/farmacologia , Polivinil/farmacologia , Siloxanas/farmacologia , Bactérias/isolamento & purificação , Parafusos Ósseos , Projeto do Implante Dentário-Pivô , Infiltração Dentária , Técnicas In Vitro , Teste de Materiais
7.
PLoS One ; 12(9): e0185512, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28957407

RESUMO

OBJECTIVE: To evaluate the effect of semiconductor laser irradiation on root canal sealing after routine root canal therapy (RCT). METHODS: Sixty freshly extracted single-rooted human teeth were randomly divided into six groups (n = 10). The anatomic crowns were sectioned at the cementoenamel junction and the remaining roots were prepared endodontically with conventional RCT methods. Groups A and B were irradiated with semiconductor laser at 1W for 20 seconds; Groups C and D were ultrasonically rinsed for 60 seconds as positive control groups; Groups E and F without treatment of root canal prior to RCT as negative control groups. Root canal sealing of Groups A, C and E were evaluated by measurements of apical microleakage. The teeth from Groups B, D and F were sectioned, and the micro-structures were examined with scanning electron microscopy (SEM). One way ANOVA and LSD-t test were used for statistical analysis (α = .05). RESULTS: The apical sealing of both the laser irradiated group and the ultrasonic irrigated group were significantly different from the control group (p<0.5). There was no significant difference between the laser irradiated group and the ultrasonic irrigated group (p>0.5). SEM observation showed that most of the dentinal tubules in the laser irradiation group melted, narrowed or closed, while most of the dentinal tubules in the ultrasonic irrigation group were filled with tooth paste. CONCLUSION: The application of semiconductor laser prior to root canal obturation increases the apical sealing of the roots treated.


Assuntos
Lasers Semicondutores , Materiais Restauradores do Canal Radicular/farmacologia , Tratamento do Canal Radicular , Corantes , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/ultraestrutura , Guta-Percha/farmacologia , Humanos , Ultrassom
8.
J Endod ; 43(7): 1137-1142, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28476469

RESUMO

INTRODUCTION: The purpose of this study was to evaluate the in vivo response of periradicular tissues after sealing of furcation perforations with Biodentine, mineral trioxide aggregate (MTA), and gutta-percha by means of histopathologic and indirect immunofluorescence analyses. METHODS: Thirty teeth of 3 dogs were divided into 3 groups: Biodentine (n = 14 teeth), MTA (negative control, n = 10 teeth), and gutta-percha (positive control, n = 6 teeth). After endodontic treatment, perforations were made on the center of the pulp chamber floor and filled with the materials. After 120 days, the animals were killed, and blocks containing the teeth and periradicular tissues were processed histotechnically for histopathologic semiquantitative (new mineralized tissue formation and bone resorption at the perforation site) and quantitative (thickness and area of newly formed mineralized tissue and number of inflammatory cells) analyses and RUNX2 immunofluorescence assay. Data were analyzed by χ2, Fisher exact test, Mann-Whitney test, one-way analysis of variance, Kruskal-Wallis test, and Dunn posttest (α = 0.05). RESULTS: MTA and Biodentine induced the formation of significantly more new mineralized tissue (P < .0001) than gutta-percha, which did not induce the formation of mineralized tissue in any case. Complete sealing of the perforations was more frequent with MTA, which formed mineralized tissue with greater thickness and area. Biodentine and MTA exhibited no bone resorption in the furcation region, fewer inflammatory cells, and greater RUNX2 immunostaining intensity than gutta-percha. CONCLUSIONS: Although MTA presented higher frequency of complete sealing and greater thickness and area of newly formed mineralized tissue, Biodentine also had good histopathologic results and can be considered as an adequate furcation perforation repair material.


Assuntos
Compostos de Cálcio/farmacologia , Cavidade Pulpar/patologia , Defeitos da Furca/terapia , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Silicatos/farmacologia , Compostos de Alumínio/farmacologia , Animais , Cavidade Pulpar/efeitos dos fármacos , Cães , Combinação de Medicamentos , Técnica Indireta de Fluorescência para Anticorpo , Defeitos da Furca/patologia , Guta-Percha/farmacologia , Óxidos/farmacologia , Periodonto/efeitos dos fármacos , Periodonto/patologia
9.
Cient. dent. (Ed. impr.) ; 14(1): 35-49, ene.-abr. 2017. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-161885

RESUMO

En el presente estudio se analizó la composición química de cuatro marcas de gutapercha (Autofit, Endosequence, Wave One Gold y Dia-ProT) para su uso en técnicas de condensación vertical con ola de calor desde un punto de vista cualitativo, utilizándose la microextracción en fase sólida con posterior análisis mediante cromatografía de gases acoplada a espectrometría de masas para realizar dicho análisis y obtener referencia de los compuestos orgánicos volátiles (COVs) presentes en las muestras. Los resultados arrojaron diferencias en la composición de las marcas, con solo cuatro COVs similares en todas las muestras. La mayoría de los compuestos encontrados tiene origen vegetal, pero resultó de interés el Butyl hydroxy Toluene (localizado en todas las muestras) que se encuentra en el Grupo 3 de la clasificación de la International Agency for Research on Cancer, en los cuales no hay evidencia de posible carcinogénesis demostrada pero su uso está siendo limitado por diversos estados. La identificación de los COVs resulta de especial interés para evitar la aparición de eventos adversos en tratamientos de endodoncia y debe tenerse en cuenta por las autoridades sanitarias a la hora de establecer controles sobre la comercialización de productos sanitarios odontológicos (AU)


In the present study chemical composition of four brands of gutta-percha (Autofit, Endosequence, Wave One Gold and Dia-ProT) was analyzed for use in warm vertical condensation techniques from a qualitative point of view, using microextraction in solid-phase with subsequent analysis by gas chromatography coupled to mass spectrometry to perform such analysis and to obtain reference of the volatile organic compounds (VOCs) present in the samples. The results showed differences in the composition of the marks, with only four similar VOCs in all the samples. Most of the compounds found are of plant origin, but Butyl hydroxy Toluene (present in all samples) belongs to Group 3 of the International Agency for Research on Cancer classification, in which there is no evidence of possible demonstrated carcinogenesis, but its use is being limited by several countries. The identification of VOCs has a special interest to avoid the occurrence of adverse events in endodontic treatments and should be consider by the health authorities when establishing controls on the commercialization of dental health products (AU)


Assuntos
Humanos , Guta-Percha/farmacologia , Composição de Medicamentos/métodos , Compostos Orgânicos Voláteis/análise , Contaminação de Medicamentos , Cromatografia Gasosa-Espectrometria de Massas
10.
Clin Oral Investig ; 21(5): 1531-1536, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27460565

RESUMO

OBJECTIVES: The aim of the present study was to evaluate cytotoxic effects and cytokine production of calcium silicate-based sealers (EndoSeal, EndoSequence BC Sealer, and MTA Fillapex) using an in vitro root canal filling model and three-dimensional (3D) cell culture. AH Plus as a reference was compared to contemporary calcium silicate cements regarding cell viability and cytokine production. MATERIAL AND METHODS: Root canals of 30 human maxillary incisors were prepared using a single-file reciprocating technique. The samples were randomly distributed and canals filled with either AH Plus, EndoSeal, EndoSequence BC Sealer, and MTA Fillapex (n = 6). In the negative control group, the root canal remained unfilled. Sealers were placed into the canals along with a gutta-percha cone placed to working length. Balb/c 3T3 fibroblasts, cultured in a type I collagen 3D scaffold, were exposed to filling material and the respective root apex for 24 h. Cytocompatibility of the materials was evaluated using the methyl-thiazoldiphenyl-tetrazolium (MTT) assay. The production of IL-1ß, IL-6, and IL-8 was analyzed using enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance was performed, and when the F-ratios were significant, data were compared by Duncan's multiple-range test. The alpha-type error was set at 0.05. RESULTS: EndoSeal, Endosequence BC Sealer and AH Plus showed cell viability that was similar to the negative control group (P > 0.05), while MTA Fillapex sealer was cytotoxic (P < 0.05). Varying production of IL-1ß, IL-6, and IL-8 was detected in all samples. CONCLUSIONS: In an in vitro root canal filling model with 3D cell culture, AH Plus, EndoSeal, and EndoSequence BC Sealer were cytocompatible. CLINICAL RELEVANCE: These results may suggest that AH Plus, EndoSeal and EndoSequence BC Sealer may achieve better biological response when compared to MTA Fillapex.


Assuntos
Materiais Biocompatíveis/farmacologia , Compostos de Cálcio/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Silicatos/farmacologia , Compostos de Alumínio/farmacologia , Compostos de Alumínio/toxicidade , Materiais Biocompatíveis/toxicidade , Compostos de Cálcio/toxicidade , Fosfatos de Cálcio , Técnicas de Cultura de Células , Células Cultivadas , Citocinas/metabolismo , Combinação de Medicamentos , Resinas Epóxi , Guta-Percha/farmacologia , Guta-Percha/toxicidade , Humanos , Técnicas In Vitro , Incisivo , Teste de Materiais , Óxidos/farmacologia , Óxidos/toxicidade , Materiais Restauradores do Canal Radicular/toxicidade , Silicatos/toxicidade , Propriedades de Superfície
11.
Int Endod J ; 50 Suppl 2: e3-e8, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27977855

RESUMO

AIM: To examine the effect of reduction in MTA particle size on dento-alveolar and osseous healing in dogs. METHODOLOGY: Root canals of 24 mandibular premolars in four 2-year-old beagles were prepared and filled with gutta-percha and sealer. Two to four weeks later, during periapical surgery, the root-end cavity preparations in these teeth were filled with either grey ProRoot MTA or modified (reduced particle sizes with faster setting time) MTA. The animals were sacrificed 4 months later. Degrees of inflammation, type of inflammatory cells, fibrous connective tissue adjacent to the root-end filling materials, cementum formation over the resected roots and root-end filling materials and bone healing were examined. Data were analysed using the McNemar test. RESULTS: No significant differences in healing of periapical tissues were found when comparing ProRoot MTA to a modified MTA containing reduced particle sizes. CONCLUSION: Reducing the particle sizes of MTA did not impact its biological properties.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Óxidos/farmacologia , Tecido Periapical/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/farmacologia , Tratamento do Canal Radicular , Silicatos/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Dente Pré-Molar/cirurgia , Cães , Combinação de Medicamentos , Guta-Percha/farmacologia , Mandíbula , Tamanho da Partícula
12.
Quintessence Int ; 48(4): 273-280, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27834415

RESUMO

OBJECTIVE: The aim of the present study was to evaluate the antimicrobial influence of different root canal filling techniques using gutta-percha and an epoxy resin-based sealer in experimentally infected root canals of extracted human teeth. METHOD AND MATERIALS: In total, 96 intact sterilized, permanent human anterior teeth and premolars with single patent root canals were prepared and infected with a clinical isolate of Enterococcus faecalis. After 72 hours, all root canals were sampled using three sterile paper points. The tooth specimens were randomly divided into three groups and a control of 24 specimens each, according to the respective obturation techniques: lateral condensation (LC group), ProTaper Thermafil (PT group), and vertical compaction technique (VC group). AH Plus was used as sealer. The control group was left untreated (without root canal filling). After 7 days root canal fillings were removed and collected. The root canals were sampled using three sterile paper points and dentin chips were obtained from the root canal walls. The samples were cultured on blood agar, and colony forming units were counted. RESULTS: All root canal filling techniques significantly reduced bacterial viability, eliminating more than 99.9% of E faecalis. In the LC group, three (13%) root canals were culture negative. In the PT group, 21 (88%) root canals and in the VC group 15 (54%) were culture negative. CONCLUSION: All root canal filling techniques significantly reduced E faecalis in root canals. In cases where warm filling techniques can be applied, these should be preferred to cold obturation.


Assuntos
Antibacterianos/farmacologia , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/microbiologia , Resinas Epóxi/farmacologia , Guta-Percha/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Preparo de Canal Radicular/métodos , Contagem de Colônia Microbiana , Resinas Compostas , Ácido Edético/farmacologia , Enterococcus faecalis , Humanos , Técnicas In Vitro , Distribuição Aleatória , Cimentos de Resina , Obturação do Canal Radicular , Hipoclorito de Sódio/farmacologia
13.
J Contemp Dent Pract ; 17(6): 445-50, 2016 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-27484596

RESUMO

INTRODUCTION: Obturation of root canal with internal resorption represents a major challenge in Endodontics. In spite of that, usual obturation techniques are often employed without considering the best technique to solve this problem. The goal of this study was to investigate the ability of GuttaFlow2 in filling artificial internal resorption cavities. MATERIALS AND METHODS: The study sample included 36 human upper central incisors that were prepared using Protaper system (F4). Internal resorption cavities were prepared by cutting each tooth at 7 mm from the apex and preparing hemispherical cavities on both the sides and then re-attaching them. The sample was randomly separated into three groups (n = 12 in each group). In the first group, thermal injection technique (Obtura II) was employed and served as the control group. In the second group, injection of cold free-flow obturation technique with a master cone (GF2-C) was employed, whereas in the third group injection of cold free-flow obturation without a master cone (GF2) was followed. The teeth were re-cut at the same level as before and examined under a stereomicroscope. Subsequently, the captured images were transferred to AutoCAD program to measure the percentage of total filling "TF," gutta-percha "G," sealer "S," and voids "V" out of the total surface of the cross sections. RESULTS: All materials showed high filling properties in terms of "total filling," ranging from 99.17% (for Obtura II) to 99.72% (for GF2-C). Regarding gutta-percha percentages of filling, they ranged from 83.15 to 83.93%, whereas those for the sealer ranged from 5.71 to 15.24%. GuttaFlow2 group with a master cone appeared to give the best results despite the insignificant differences among the three groups. CONCLUSION: The GuttaFlow2 with a master cone technique seemed to be a promising filling material and gave results similar to those observed with Obtura II. It is recommended for use to obturate internal resorption cavities in clinical practice due to its good adaptability to root canal walls, ease of handling, and application. CLINICAL SIGNIFICANCE: Internal resorption defects can be successfully filled with GuttaFlow2 material when supplemented with a master cone, and the results are comparable with those obtained with the Obtura II technique.


Assuntos
Dimetilpolisiloxanos/farmacologia , Guta-Percha/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Obturação do Canal Radicular/métodos , Combinação de Medicamentos , Humanos , Técnicas In Vitro , Incisivo
14.
J Appl Biomater Funct Mater ; 14(3): e307-13, 2016 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-27149939

RESUMO

BACKGROUND: The aim of this study was to evaluate and compare the obturation quality between canals obturated with gutta-percha/AH Plus sealer (GP group) and resin-coated GP/EndoREZ® sealer (ER group). METHODS: A total sample of 90 mandibular premolar teeth was divided into 2 groups (2 × 45 canals): the GP group and ER group. Each group was further divided into 3 subgroups (n = 15): cold lateral compaction (CLC), warm lateral compaction (WLC) and single cone (SC). The teeth were subsequently embedded in resin and sectioned horizontally at 1, 3, 6 and 9 mm. All sections were then viewed with a stereomicroscope at ×40 magnification. The area occupied by core filling materials was determined using Cell^D software. RESULTS: With CLC, the percentage of core filling materials in the ER group was significantly higher than in the GP group at the 1- and 3-mm levels. Similarly, with WLC, the percentage of core filling material in the ER group was significantly higher than in the GP group at the 1-, 3- and 9-mm levels. With SC, the percentage of core filling materials in the ER group was significantly higher than in the GP group at all levels. CONCLUSIONS: It can be concluded that the resin-coated GP/EndoREZ® sealer is superior to the gutta-percha/AH Plus in the percentage of core filling material.


Assuntos
Dente Pré-Molar , Guta-Percha/química , Resinas Sintéticas/química , Preparo de Canal Radicular , Feminino , Guta-Percha/farmacologia , Humanos , Masculino , Resinas Sintéticas/farmacologia
15.
Braz Oral Res ; 302016.
Artigo em Inglês | MEDLINE | ID: mdl-26910015

RESUMO

The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.


Assuntos
Antibacterianos/farmacologia , Dimetilpolisiloxanos/farmacologia , Enterococcus faecalis/efeitos dos fármacos , Resinas Epóxi/farmacologia , Guta-Percha/farmacologia , Materiais Restauradores do Canal Radicular/farmacologia , Prata/farmacologia , Antibacterianos/química , Contagem de Colônia Microbiana , Dimetilpolisiloxanos/química , Combinação de Medicamentos , Resinas Epóxi/química , Guta-Percha/química , Teste de Materiais , Tamanho da Partícula , Reprodutibilidade dos Testes , Materiais Restauradores do Canal Radicular/química , Silicones/química , Silicones/farmacologia , Prata/química , Estatísticas não Paramétricas , Fatores de Tempo
16.
Mil Med ; 180(3 Suppl): 92-5, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25747639

RESUMO

Dental emergencies negatively affect troop readiness, especially during combat. Endodontic retreatment, when required, is especially challenging when the removal of endodontic sealer is required. In this study, we investigated the effectiveness of synthetic endodontic solvents to remove endodontic sealers. Fifty capillary tubes (2.7 mm ID×22 mm L), each filled to 15 mm with either Roth 801, AH Plus, MetaSEAL, or gutta-percha, were stored at 75% humidity for 14 days at 37°C. Ten capillary tubes containing each sealer were treated with either chloroform, xylene, EndoSolv R, EndoSolv E, or no solvent, and then penetrated with D3 ProTaper Universal Retreatment file on the same day. The time for the file to penetrate the length of each sealer was recorded, and the data statistically analyzed. Roth 801 failed to set and was not tested. The file took 3.4±0.1, 4.8±0.3, 5.7±0.4, 4.5±0.2, and 10.6±1.0 seconds (mean±SD) to penetrate gutta-percha using chloroform, xylene, EndoSolv R, EndoSolv E, or no solvent, respectively, and was performed by one endodontic resident at one sitting. The time for penetration of gutta-percha with any solvent was significantly faster (p≤0.05) than for AH Plus or MetaSEAL.The time for AH Plus ranged from 23.1±1.0 to 81.5±4.5 seconds. The time for MetaSEAL ranged from 97.2±6.1 to >180 seconds. EndoSolv E was the most effective solvent for AH Plus. It took significantly more time to remove MetaSEAL than AH Plus, regardless of the solvent used. Our study indicated that the use of the proper endodontic solvent makes complete removal of a sealer much more effective during retreatment.


Assuntos
Guta-Percha/farmacologia , Militares , Materiais Restauradores do Canal Radicular/farmacologia , Solventes/farmacologia , Doenças Dentárias/terapia , Cimento de Óxido de Zinco e Eugenol/farmacologia , Humanos , Retratamento
17.
J Endod ; 41(2): 257-60, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25433971

RESUMO

INTRODUCTION: The purpose of this study was to evaluate the effect of final irrigation with a new endodontic irrigant, QMix 2in1 (Dentsply Tulsa Dental, Tulsa, OK), on sealer penetration into dentinal tubules using confocal laser scanning microscopy. METHODS: Thirty recently extracted human maxillary incisors were used in the present study. The teeth were instrumented with sodium hypochlorite (NaOCl) irrigation and then divided into 3 groups according to the final irrigation regimen used: (1) control group, 2.5% NaOCl; (2) EDTA + CHX group, 17% (EDTA) followed by 2% chlorhexidine (CHX); and (3) QMix group, QMix 2in1. All teeth were dried and then obturated with gutta-percha and rhodamine B-labeled AH26 sealer (Dentsply DeTrey, Konstanz, Germany). After setting, the roots were sectioned horizontally 3, 5, and 8 mm from the root apex. Sealer penetration into the dentinal tubules was examined by confocal laser scanning microscopy. RESULTS: In the apical sections of the roots, no significant difference was detected in the percentage of sealer penetration between any of the final irrigation groups (P > .05). Both the EDTA + CHX and QMix groups showed significantly more sealer penetration than the control group in the middle and coronal sections. However, no difference was found between the EDTA + CHX and QMix groups in either of these areas (P > .05) CONCLUSIONS: Use of EDTA + CHX or QMix during final irrigation significantly improved sealer penetration when compared with the control group in the middle and coronal sections of the roots; however, no effect was observed in the apical sections.


Assuntos
Biguanidas/farmacologia , Dentina/efeitos dos fármacos , Incisivo/efeitos dos fármacos , Polímeros/farmacologia , Irrigantes do Canal Radicular/farmacologia , Dentina/diagnóstico por imagem , Dentina/patologia , Dentina/ultraestrutura , Guta-Percha/farmacologia , Humanos , Incisivo/diagnóstico por imagem , Incisivo/ultraestrutura , Microscopia Confocal , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular , Hipoclorito de Sódio/farmacologia
18.
J Endod ; 40(11): 1869-73, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25190606

RESUMO

INTRODUCTION: The purpose of this study was to evaluate the biocompatibility of a self-adhesive gutta-percha material and compare it with that of conventional gutta-percha. METHODS: Standard quantities of bioactive gutta-percha and conventional gutta-percha were directly inserted subcutaneously into the dorsal connective tissue of 30 BALB/c mice according to ISO 10993-6. After 7, 21, and 63 days each, 10 animals were euthanized, and the materials and surrounding tissue were removed. Tissue samples were subjected to histological processing resulting in 5-µm-thick slices stained with hematoxylin-eosin and Gomori trichrome stain. A grade ranging from I-IV was used to classify the inflammatory reaction. The Mann-Whitney U test with Bonferroni correction was used to compare the grade of inflammation induced by the materials at each time point. Qualitative evaluation of biocompatibility over time was also performed. RESULTS: Bioactive gutta-percha was more biocompatible than conventional gutta-percha at each time interval (P < .05). Tissue exposed to bioactive gutta-percha reached "no inflammation" (grade I) at the 21-day interval, whereas it took 63 days for the conventional gutta-percha to reach the "slight inflammation" level (grade II). CONCLUSIONS: Bioactive gutta-percha presented good tissue reaction at all time points. It may serve as an alternative to gutta-percha in terms of biocompatibility.


Assuntos
Materiais Biocompatíveis/farmacologia , Guta-Percha/farmacologia , Tela Subcutânea/efeitos dos fármacos , Animais , Materiais Biocompatíveis/química , Celulite (Flegmão)/patologia , Colágeno/análise , Edema/patologia , Fibrina/análise , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Fibrose , Guta-Percha/química , Hemiterpenos/química , Látex/química , Macrófagos/efeitos dos fármacos , Macrófagos/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Nanopartículas/química , Neutrófilos/patologia , Tela Subcutânea/anatomia & histologia , Fatores de Tempo , Óxido de Zinco/química
19.
J Endod ; 40(9): 1424-8, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25146025

RESUMO

OBJECTIVE: This study evaluated the response of apical and periapical tissues of dogs' teeth with pulp vitality after root canal filling with the endodontic sealers Sealapex Xpress and Real Seal XT. METHODS: Thirty-eight root canals with vital pulp from dogs' premolars were used. After instrumentation, the canals were filled with Sealapex Xpress and gutta-percha (group SX/GP, n = 16) or Real Seal XT and Resilon cones (group RS/R, n = 22). The animals were killed after 90 days, and the teeth with surrounding tissues were subjected to histotechnical processing. Hematoxylin-eosin-stained sections were examined by conventional light microscopy for a quantitative histopathologic analysis (sealing of apical opening by newly formed mineralized tissue [biological sealing], inflammatory cell infiltrate, root and bone tissue resorption), according to a scoring system. The subsequent sections were evaluated by immunohistochemistry for identification of mineralization markers (osteopontin, alkaline phosphatase, and RUNX2). Data were analyzed by nonparametric Mann-Whitney U test (α = 0.05). RESULTS: Complete biological sealing was observed in 50% and 22.7% of the specimens of groups SX/GP and RS/R, respectively. Partial biological sealing was observed in 25% and 54.6% and absence of sealing in 25% and 22.7% of the specimens of groups SX/GP and RS/R, respectively. There were no significant differences (P > .05) between the groups for the scores attributed to the histopathologic parameters. Positive staining for osteopontin, alkaline phosphatase, and RUNX2 was observed in both groups, especially in the periodontal ligament. CONCLUSIONS: Sealapex Xpress and RealSeal XT feature tissue compatibility in vivo and allow for sealing of apical opening by deposition of mineralized tissue.


Assuntos
Materiais Biocompatíveis/farmacologia , Hidróxido de Cálcio/farmacologia , Resinas Compostas/farmacologia , Tecido Periapical/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/farmacologia , Salicilatos/farmacologia , Fosfatase Alcalina/análise , Perda do Osso Alveolar/patologia , Animais , Apexificação/métodos , Subunidade alfa 1 de Fator de Ligação ao Core/análise , Colagem Dentária/métodos , Cães , Guta-Percha/farmacologia , Inflamação/patologia , Osteopontina/análise , Ligamento Periodontal/efeitos dos fármacos , Obturação do Canal Radicular/métodos , Preparo de Canal Radicular/métodos , Reabsorção da Raiz/patologia
20.
J Endod ; 40(4): 505-8, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24666900

RESUMO

INTRODUCTION: The purpose of this study was to evaluate the antimicrobial effects of root canal sealers on Enterococcus faecalis biofilms in dentinal tubules by using a novel dentin infection model. METHODS: Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion broth for 3 weeks. An equal thickness of AH Plus, Endosequence BC sealer (BC sealer), and pulp canal sealer EWT (PCEWT) was placed on the root canal wall of the dentin specimens for 1, 7, and 30 days in humid conditions at 37°C. Gutta-percha and water were used in a similar manner as the tested sealers. The proportions of dead and live bacteria inside the dentinal tubules after exposure to root canal sealers were assessed by confocal laser scanning microscopy. RESULTS: Significantly more bacteria were killed in the 3 sealer groups than in the 2 control groups (P < .05). BC sealer and AH Plus resulted in significantly more dead cells than PCEWT did. There was no statistically significant difference between BC sealer and AH Plus at any time point (P > .05). Thirty days of exposure to BC sealer and AH Plus resulted in significantly more dead bacteria in dentin than 7-day and 1-day exposures in the biofilms, whereas no statistically significant increase of the proportion of dead bacteria was detected between 7-day and 30-day PCEWT (P > .05). CONCLUSIONS: The 3 endodontic root canal sealers had antibacterial effects against E. faecalis in the dentinal tubules. BC sealer and AH Plus had superior antibacterial effects compared with PCEWT. The antibacterial effects of sealers in dentinal tubules continued after setting.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Dentina/microbiologia , Enterococcus faecalis/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/farmacologia , Técnicas Bacteriológicas , Fosfatos de Cálcio/farmacologia , Dentina/ultraestrutura , Combinação de Medicamentos , Resinas Epóxi/farmacologia , Guta-Percha/farmacologia , Humanos , Umidade , Viabilidade Microbiana/efeitos dos fármacos , Microscopia Confocal , Óxidos/farmacologia , Distribuição Aleatória , Silicatos/farmacologia , Fatores de Tempo , Técnicas de Cultura de Tecidos , Cimento de Óxido de Zinco e Eugenol/farmacologia
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