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1.
BMC Genomics ; 25(1): 682, 2024 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-38982341

RESUMO

BACKGROUND: Green foxtail [Setaria viridis (L.)] is one of the most abundant and troublesome annual grass weeds in alfalfa fields in Northeast China. Synthetic auxin herbicide is widely used in agriculture, while how auxin herbicide affects tillering on perennial grass weeds is still unclear. A greenhouse experiment was conducted to examine the effects of auxin herbicide 2,4-D on green foxtail growth, especially on tillers. RESULTS: In the study, 2,4-D isooctyl ester was used. There was an inhibition of plant height and fresh weight on green foxtail after application. The photosynthetic rate of the leaves was dramatically reduced and there was an accumulation of malondialdehyde (MDA) content. Moreover, applying 2,4-D isooctyl ester significantly reduced the tillering buds at rates between 2100 and 8400 ga. i. /ha. Transcriptome results showed that applying 2,4-D isooctyl ester on leaves affected the phytohormone signal transduction pathways in plant tillers. Among them, there were significant effects on auxin, cytokinin, abscisic acid (ABA), gibberellin (GA), and brassinosteroid signaling. Indeed, external ABA and GA on leaves also limited tillering in green foxtail. CONCLUSIONS: These data will be helpful to further understand the responses of green foxtail to 2, 4-D isooctyl ester, which may provide a unique perspective for the development and identification of new target compounds that are effective against this weed species.


Assuntos
Ácido 2,4-Diclorofenoxiacético , Herbicidas , Reguladores de Crescimento de Plantas , Setaria (Planta) , Ácido 2,4-Diclorofenoxiacético/farmacologia , Setaria (Planta)/efeitos dos fármacos , Setaria (Planta)/genética , Setaria (Planta)/metabolismo , Setaria (Planta)/crescimento & desenvolvimento , Reguladores de Crescimento de Plantas/farmacologia , Reguladores de Crescimento de Plantas/metabolismo , Herbicidas/farmacologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/metabolismo , Ácidos Indolacéticos/metabolismo , Ácidos Indolacéticos/farmacologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Fotossíntese/efeitos dos fármacos , Giberelinas/farmacologia , Giberelinas/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transcriptoma/efeitos dos fármacos , Ésteres
2.
Ren Fail ; 46(2): 2374013, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38967153

RESUMO

OBJECTIVE: To evaluate the clinical efficacy and safety of fractionated plasma separation and adsorption combined with continuous veno-venous hemofiltration (FPSA-CVVH) treatment in patients with acute bipyridine herbicide poisoning. METHODS: A retrospective analysis of 18 patients with acute bipyridine herbicide poisoning was conducted, of which 9 patients were poisoned by diquat and 9 patients by paraquat. All patients underwent FPSA-CVVH treatment. The serum cytokine levels in pesticide-poisoned patients were assessed. The efficacy of FPSA-CVVH in eliminating cytokines, the 90-d survival rate of poisoned patients, and adverse reactions to the treatment were observed. RESULTS: Fourteen patients (77.8%) had acute kidney injuries and 10 (55.6%) had acute liver injuries. The serum cytokine levels of high mobility group protein B-1 (HMGB-1), interleukin-6 (IL-6), IL-8, interferon-inducible protein-10 (IP-10), monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-1ß (MIP-1ß) were significantly elevated. A total of 41 FPSA-CVVH treatment sessions were administered. After a single 8-h FPSA-CVVH treatment, the decreases in HMGB-1, IL-6, IL-8, IP-10, MCP-1, and MIP-1ß were 66.0%, 63.5%, 73.3%, 63.7%, 53.9%, and 54.1%, respectively. During FPSA-CVVH treatment, one patient required a filter change due to coagulation in the plasma component separator, and one experienced a bleeding adverse reaction. The 90-d patient survival rate was 50%, with 4 patients with diquat poisoning and 5 patients with paraquat poisoning, and both liver and kidney functions were restored to normal. CONCLUSION: Cytokine storms may play a significant role in the progression of multiorgan dysfunction in patients with acute bipyridine herbicide poisoning. FPSA-CVVH can effectively reduce cytokine levels, increase the survival rate of patients with acute bipyridine herbicide poisoning, and decrease the incidence of adverse events.


Assuntos
Injúria Renal Aguda , Terapia de Substituição Renal Contínua , Herbicidas , Humanos , Masculino , Feminino , Herbicidas/intoxicação , Estudos Retrospectivos , Adulto , Pessoa de Meia-Idade , Injúria Renal Aguda/terapia , Injúria Renal Aguda/induzido quimicamente , Citocinas/sangue , Paraquat/intoxicação , Diquat/intoxicação , Adulto Jovem , Idoso , Hemofiltração/métodos , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Doença Hepática Induzida por Substâncias e Drogas/terapia
3.
Int J Mol Sci ; 25(13)2024 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-39000147

RESUMO

Glyphosate, the active ingredient of several broad-spectrum herbicides, is widely used throughout the world, although many adverse effects are known. Among these, it has been recognized as an endocrine disruptor. This work aimed to test the effects and potential endocrine disrupting action of glyphosate on PNT1A human prostate cells, an immortalized non-tumor epithelial cell line, possessing both ERα and ERß estrogen receptors. The results showed that glyphosate induces cytotoxicity, mitochondrial dysfunction, and rapid activation of ERα and ERß via nuclear translocation. Molecular analysis indicated a possible involvement of apoptosis in glyphosate-induced cytotoxicology. The apoptotic process could be attributed to alterations in mitochondrial metabolism; therefore, the main parameters of mitochondrial functionality were investigated using the Seahorse analyzer. Impaired mitochondrial function was observed in glyphosate-treated cells, with reductions in ATP production, spare respiratory capacity, and proton leakage, along with increased efficiency of mitochondrial coupling. Finally, the results of immunofluorescence analysis demonstrated that glyphosate acts as an estrogen disruptor determining the nuclear translocation of both ERs. Nuclear translocation occurred independent of dose, faster than the specific hormone, and persisted throughout treatment. In conclusion, the results collected show that in non-tumor prostate cells glyphosate can cause cell death and acts as a xenoestrogen, activating estrogen receptors. The consequent alteration of hormonal functions can have negative effects on the reproductive health of exposed animals, compromising their fertility.


Assuntos
Apoptose , Receptor alfa de Estrogênio , Receptor beta de Estrogênio , Glicina , Glifosato , Mitocôndrias , Próstata , Glicina/análogos & derivados , Glicina/farmacologia , Glicina/toxicidade , Humanos , Masculino , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Receptor beta de Estrogênio/metabolismo , Receptor alfa de Estrogênio/metabolismo , Próstata/efeitos dos fármacos , Próstata/metabolismo , Próstata/patologia , Apoptose/efeitos dos fármacos , Linhagem Celular , Herbicidas/toxicidade , Disruptores Endócrinos/toxicidade , Disruptores Endócrinos/farmacologia , Sobrevivência Celular/efeitos dos fármacos
4.
Sci Total Environ ; 946: 174343, 2024 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-38960172

RESUMO

The structure and biomass of aquatic invertebrate communities play a crucial role in the matter dynamics of streams. However, biomass is rarely quantified in ecological assessments of streams, and little is known about the environmental and anthropogenic factors that influence it. In this study, we aimed to identify environmental factors that are associated with invertebrate structure and biomass through a monitoring of 25 streams across Germany. We identified invertebrates, assigned them to taxonomic and trait-based groups, and quantified biomass using image-based analysis. We found that insecticide pressure generally reduced the abundance of insecticide-vulnerable populations (R2 = 0.43 applying SPEARpesticides indicator), but not invertebrate biomass. In contrast, herbicide pressure reduced the biomass of several biomass aggregations. Especially, insecticide-sensitive populations, that were directly (algae feeder, R2 = 0.39) or indirectly (predators, R2 = 0.29) dependent on algae, were affected. This indicated a combined effect of possible food shortage due to herbicides and direct insecticide pressure. Specifically, all streams with increased herbicide pressure showed a reduced overall biomass share of Trichoptera from 43 % to 3 % and those of Ephemeroptera from 20 % to 3 % compared to streams grouped by low herbicide pressure. In contrast, insecticide-insensitive Gastropoda increased from 10 % to 45 %, and non-vulnerable leaf-shredding Crustacea increased from 10 % to 22 %. In summary, our results indicate that at the community level, the direct effects of insecticides and the indirect, food-mediated effects of herbicides exert a combined effect on the biomass of sensitive insect groups, thus disrupting food chains at ecosystem level.


Assuntos
Biomassa , Monitoramento Ambiental , Herbicidas , Inseticidas , Invertebrados , Poluentes Químicos da Água , Animais , Herbicidas/toxicidade , Invertebrados/efeitos dos fármacos , Invertebrados/fisiologia , Inseticidas/toxicidade , Poluentes Químicos da Água/toxicidade , Alemanha , Organismos Aquáticos/efeitos dos fármacos , Rios/química
5.
Environ Toxicol Pharmacol ; 109: 104496, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38959819

RESUMO

Endocrine disruptors (EDs) pose significant risks to human and environmental health, with potential implications for neurotoxicity. This study investigates the synergistic neurotoxic effects of perfluorooctane sulfonate (PFOS) and glyphosate (GLY), two ubiquitous EDs, using SHSY5Y neuronal and C6 astrocytic cell lines. While individual exposures to PFOS and glyphosate at non-toxic concentrations did not induce significant changes, their combination resulted in a marked increase in oxidative stress and neuroinflammatory responses. Specifically, the co-exposure led to elevated levels of interleukin-6, tumor necrosis factor alpha, and interferon gamma, along with reduced interleukin-10 expression, indicative of heightened neuroinflammatory processes. These findings underscore the importance of considering the synergistic interactions of EDs in assessing neurotoxic risks and highlight the urgent need for further research to mitigate the adverse effects of these compounds on neurological health.


Assuntos
Ácidos Alcanossulfônicos , Disruptores Endócrinos , Fluorocarbonos , Glicina , Glifosato , Glicina/análogos & derivados , Glicina/toxicidade , Fluorocarbonos/toxicidade , Disruptores Endócrinos/toxicidade , Humanos , Ácidos Alcanossulfônicos/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Linhagem Celular Tumoral , Linhagem Celular , Animais , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Astrócitos/efeitos dos fármacos , Astrócitos/metabolismo , Herbicidas/toxicidade , Citocinas/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Ratos
6.
Molecules ; 29(13)2024 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-38998991

RESUMO

Weeds are a serious threat to crop production, and the utilization of secondary metabolites of phytopathogenic fungi is considered to be an effective method of weed control. In this study, eight compounds were isolated and purified from the mycelium and fermentation broth extracts of Bipolaris cookei SYBL03. The compounds (1-8), except 2 and 6, are reported for the first time from this genus. The herbicidal activities of compounds 1-8 were studied by evaluating their effects on the seed germination and seedling growth of monocotyledonous and dicotyledonous weeds. The results indicated that compound 7 (Cyclo-N-methylphenylalanyltryptophenyl, cNMPT) exhibited a concentration-dependent dual effect on the growth of weed seedlings and selective herbicidal activity against dicotyledonous weeds. We further investigated the morphological and physiological responses of roots of Amaranthus retroflexus, a dicotyledonous weed, to compound 7. Some changes were found in seedlings grown in 400 µg/mL compound 7 solution for 96 h, such as shortening and swelling of elongation zone cells, reduced number and length of root hairs, damage and wrinkling of the root surface, occurrence of electrolyte leakage, and an increase in ethylene content. These results suggest that compound 7 may exert herbicidal activity by causing stress to weed seedlings. Increased ethylene production could be involved in the response of plants to compound 7.


Assuntos
Bipolaris , Herbicidas , Plantas Daninhas , Plântula , Herbicidas/farmacologia , Herbicidas/química , Herbicidas/isolamento & purificação , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Bipolaris/efeitos dos fármacos , Plantas Daninhas/efeitos dos fármacos , Plantas Daninhas/crescimento & desenvolvimento , Germinação/efeitos dos fármacos , Amaranthus/efeitos dos fármacos , Amaranthus/crescimento & desenvolvimento , Raízes de Plantas , Micélio/efeitos dos fármacos , Micélio/crescimento & desenvolvimento
7.
Ecotoxicol Environ Saf ; 281: 116646, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38954906

RESUMO

Agricultural production relies heavily on the use of pesticides, which may accumulate in soil and water, posing a significant threat to the global ecological environment and biological health. Butachlor is a commonly used herbicide and environmental pollutant, which has been linked to liver and kidney damage, as well as neurological abnormalities. However, the potential impact of butachlor exposure on the gut microbiota remains understudied. Thus, our aim was to investigate the potential negative effects of butachlor exposure on host health and gut microbiota. Our results demonstrated that butachlor exposure significantly reduced the host antioxidant capacity, as evidenced by decreased levels of T-AOC, SOD, and GSH-Px, and increased levels of MDA. Serum biochemical analysis also revealed a significant increase in AST and ALT levels during butachlor exposure. Microbial analysis showed that butachlor exposure significantly reduced the abundance and diversity of gut microbiota. Furthermore, butachlor exposure also significantly altered the gut microbial composition. In conclusion, our findings indicate that butachlor exposure can have detrimental health effects, including dysregulation of antioxidant enzymes, abnormalities in transaminases, and hepatointestinal damage. Furthermore, it disrupts the gut microbial homeostasis by altering microbial composition and reducing diversity and abundance. In the context of the increasingly serious use of pesticides, this study will help provide impetus for standardizing the application of pesticides and reducing environmental pollution.


Assuntos
Acetanilidas , Microbioma Gastrointestinal , Homeostase , Microbioma Gastrointestinal/efeitos dos fármacos , Homeostase/efeitos dos fármacos , Animais , Acetanilidas/toxicidade , Herbicidas/toxicidade , Praguicidas/toxicidade , Masculino , Antioxidantes/metabolismo , Poluentes Ambientais/toxicidade
8.
Theor Appl Genet ; 137(7): 176, 2024 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-38969812

RESUMO

Circular RNAs (circRNAs), a class of non-coding RNA molecules, are recognized for their unique functions; however, their responses to herbicide stress in Brassica napus remain unclear. In this study, the role of circRNAs in response to herbicide treatment was investigated in two rapeseed cultivars: MH33, which confers non-target-site resistance (NTSR), and EM28, which exhibits target-site resistance (TSR). The genome-wide circRNA profiles of herbicide-stressed and non-stressed seedlings were analyzed. The findings indicate that NTSR seedlings exhibited a greater abundance of circRNAs, shorter lengths of circRNAs and their parent genes, and more diverse functions of parent genes compared with TSR seedlings. Compared to normal-growth plants, the herbicide-stressed group exhibited similar trends in the number of circRNAs, functions of parent genes, and differentially expressed circRNAs as observed in NTSR seedlings. In addition, a greater number of circRNAs that function as competing microRNA (miRNA) sponges were identified in the herbicide stress and NTSR groups compared to the normal-growth and TSR groups, respectively. The differentially expressed circRNAs were validated by qPCR. The differntially expressed circRNA-miRNA networks were predicted, and the mRNAs targeted by these miRNAs were annotated. Our results suggest that circRNAs play a crucial role in responding to herbicide stress, exhibiting distinct responses between NTSR and TSR in rapeseed. These findings offer valuable insights into the mechanisms underlying herbicide resistance in rapeseed.


Assuntos
Brassica napus , Regulação da Expressão Gênica de Plantas , Resistência a Herbicidas , Herbicidas , RNA Circular , RNA de Plantas , Brassica napus/genética , Brassica napus/efeitos dos fármacos , Brassica napus/crescimento & desenvolvimento , RNA Circular/genética , Herbicidas/farmacologia , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , RNA de Plantas/genética , Resistência a Herbicidas/genética , Plântula/genética , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Estresse Fisiológico/genética , MicroRNAs/genética , MicroRNAs/metabolismo , Genoma de Planta
9.
J Water Health ; 22(6): 1088-1101, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38935459

RESUMO

Despite the negative effects that the use of pesticides (such as herbicides and insecticides) have on human health and water resources, a significant portion of the world's agricultural production depends on them. The purpose of this study was to determine selected residual concentrations of pesticides (diazinon, ethion, malathion, alachlor, methyl-parathion, trifluralin, atrazine, chlorpyrifos, and azinphos-methyl) in samples from Shiraz potable water sources. For this purpose, water treatment plant, groundwater wells, treated surface water, and a mixture of groundwater and treated surface water were taken. In addition, statistical and risk analyses (carcinogenic and non-carcinogenic) were used. According to the results, chlorpyrifos with 84.4% had the highest removal efficiency and methyl-parathion with 10% had the lowest removal rate in the Shiraz water treatment plant process. The highest mean concentration was related to azinphos-methyl (1.5 µg/L) and chlorpyrifos (0.59 µg/L) in the groundwater samples. All measured compounds in water source samples were below standard levels, except for chlorpyrifos and azinphos-methyl, which were reported in groundwater above the limit recommended by the Environmental Protection Agency (EPA). The results showed that while the selected pesticides measured had a low non-carcinogenic risk for both adults and children, malathion and trifluralin posed a high carcinogenic risk for adults.


Assuntos
Monitoramento Ambiental , Água Subterrânea , Herbicidas , Inseticidas , Poluentes Químicos da Água , Purificação da Água , Poços de Água , Poluentes Químicos da Água/análise , Herbicidas/análise , Medição de Risco , Inseticidas/análise , Água Subterrânea/química , Água Subterrânea/análise , Água Potável/análise , Água Potável/química , Humanos , Irã (Geográfico)
10.
J Hazard Mater ; 474: 134771, 2024 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-38861898

RESUMO

Nickel oxide nanoparticles (NiO-NPs) are common nanomaterials that may be released into the environment, affecting the toxicity of other contaminants. Atrazine (ATZ) is a commonly used herbicide that can harm organisms due to its persistence and bioaccumulation in the environment. Although the toxicity of ATZ to earthworms is well-documented, the risk of co-exposure with NiO-NPs increases as more nanoparticles accumulate in the soil. In this study, we investigated the effects and mechanisms of NiO-NPs on the accumulation of ATZ in earthworms. The results showed that after day 21, the antioxidant system of the cells under ATZ treatment alone was adversely affected, with ROS content 36.05 % higher than that of the control (CK) group. However, the addition of NiO-NPs reduced the ROS contents in the earthworms by 0.6 %- 32.3 %. Moreover, analysis of earthworm intestinal sections indicates that NiO-NPs mitigated cellular and tissue damage caused by ATZ. High-throughput sequencing revealed that NiO-NPs in earthworm intestines increased the abundance of Pseudomonas aeruginosa and Aeromonas aeruginosa. Additionally, the enhanced function of the ABC transport system in the gut resulted in lower accumulation of ATZ in earthworms. In summary, NiO-NPs can reduce the accumulation and thus the toxicity of ATZ in earthworms. Our study contributes to a deeper understanding of the effects of NiO-NPs on co-existing pollutants.


Assuntos
Atrazina , Herbicidas , Nanopartículas Metálicas , Níquel , Oligoquetos , Espécies Reativas de Oxigênio , Poluentes do Solo , Oligoquetos/efeitos dos fármacos , Oligoquetos/metabolismo , Atrazina/toxicidade , Animais , Níquel/toxicidade , Herbicidas/toxicidade , Poluentes do Solo/toxicidade , Poluentes do Solo/metabolismo , Nanopartículas Metálicas/toxicidade , Espécies Reativas de Oxigênio/metabolismo
11.
Pestic Biochem Physiol ; 202: 105966, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38879343

RESUMO

Atrazine (ATR) is the second most extensively used herbicide which adversely affects the body organs including liver. Salvigenin (SGN) is a flavonoid which demonstrates a wide range of biological and pharmacological abilities. This study was planned to assess the protective ability of SGN to avert ATR induced liver damage in rats. Thirty-two rats (Rattus norvegicus) were divided into four groups including control, ATR (5 mg/kg), ATR (5 mg/kg) + SGN (10 mg/kg) and SGN (10 mg/kg) alone supplemented group. ATR exposure reduced the expression of Nrf-2 while instigating an upregulation in Keap-1 expression. Furthermore, the activities of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), heme­oxygenase-1 (HO-1) and glutathione reductase (GSR) contents were decreased while increasing reactive oxygen species (ROS) and malondialdehyde (MDA) levels after ATR treatment. Moreover, ATR poisoning increased the levels of ALT, AST, and ALP while reducing the levels of total proteins, and albumin in hepatic tissues of rats. Besides, ATR administration escalated the expressions of Bax and Caspase-3 while inducing a downregulation in the expressions of Bcl-2. Similarly, ATR intoxication increased the levels of Interleukin-6 (IL-6), Nuclear factor kappa-B (NF-κB), Interleukin-1ß (IL-1ß), tumor necrosis factor-α (TNF-α), and the activity of cyclooxygenase-2 (COX-2). Furthermore, ATR disrupted the normal histology of hepatic tissues. However, SGN treatment remarkably protected the liver tissues via regulating antioxidant, anti, inflammatory, anti-apoptotic as well as histology parameters. Therefore, it is concluded that SGN can be used as therapeutic agent to combat ATR-induced hepatotoxicity.


Assuntos
Atrazina , Doença Hepática Induzida por Substâncias e Drogas , Proteína 1 Associada a ECH Semelhante a Kelch , Fígado , Fator 2 Relacionado a NF-E2 , NF-kappa B , Animais , Atrazina/toxicidade , Fator 2 Relacionado a NF-E2/metabolismo , NF-kappa B/metabolismo , Ratos , Masculino , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Proteína 1 Associada a ECH Semelhante a Kelch/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Herbicidas/toxicidade , Transdução de Sinais/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Isoflavonas/farmacologia , Espécies Reativas de Oxigênio/metabolismo
12.
J Toxicol Environ Health A ; 87(18): 719-729, 2024 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-38884257

RESUMO

Corn is the second most cultivated crop in Brazil, the number-one country in pesticide consumption. Chemical control of weeds is performed using herbicides such as S-metolachlor with pre- and post-emergence action and thus the toxicity of herbicides constitutes a matter of great concern. The present investigation aimed to examine the effects of an S-metolachlor-based herbicide on Lactuca sativa L. (lettuce) and Zea mays L. (maize) utilizing various bioassays. The test solutions were prepared from commercial products containing the active ingredient. Seeds from the plant models were exposed in petri dishes and maintained under biochemical oxygen demand (BOD) at 24°C. Distilled water was negative and aluminium positive control. Macroscopic analyses (germination and growth) were conducted for both plant species, and microscopic analysis (cell cycle and chromosomal alterations) were performed for L. sativa root tip cells. Detrimental interference of S-metolachlor-based herbicide was noted with lettuce for all parameters tested reducing plant germination by over 50% and the germination speed by over 45% and showing a significant decrease in mitotic index, from 16.25% to 9,28% even on the lowest concentration tested. In maize, there was no significant interference in plant germination; however, speed of germination was significantly hampered, reaching a 51.22% reduction for the highest concentration tested. Data demonstrated that the herbicide was toxic as evidenced by its phyto- and cytotoxicity in L. sativa L. and Z. mays L.


Assuntos
Acetamidas , Herbicidas , Lactuca , Zea mays , Zea mays/efeitos dos fármacos , Herbicidas/toxicidade , Lactuca/efeitos dos fármacos , Lactuca/crescimento & desenvolvimento , Acetamidas/toxicidade , Germinação/efeitos dos fármacos , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento
13.
PLoS One ; 19(6): e0304863, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38905259

RESUMO

Echinochloa crus-galli is a serious weed species in rice paddies. To obtain a new potential bioherbicide, we evaluated the inhibitory activities of 13 essential oils and their active substances against E. crus-galli. Essential oil from Syzygium aromaticum (L.) Merr. & L. M. Perry (SAEO) exhibited the highest herbicidal activity (EC50 = 3.87 mg mL-1) among the 13 essential oils evaluated. The SAEO was isolated at six different temperatures by vacuum fractional distillation, including 164°C, 165°C (SAEO-165), 169°C, 170°C 175°C and 180°C. The SAEO-165 had the highest inhibitory rate against E. crus-galli. Gas chromatography-mass spectrometry and high phase liquid chromatography identified eugenol (EC50 = 4.07 mg mL-1), α-caryophyllene (EC50 = 17.34 mg mL-1) and ß-caryophyllene (EC50 = 96.66 mg mL-1) as the three compounds in SAEO. Results from a safety bioassay showed that the tolerance of rice seedling (~ 20% inhibition) was higher than that of E. crus-galli (~ 70% inhibition) under SAEO stress. SAEO induced excessive generation of reactive oxygen species leading to oxidative stress and ultimately tissue damage in E. crus-galli. Our results indicate that SAEO has a potential for development into a new selective bio-herbicide. They also provide an example of a sustainable management strategy for E. crus-galli in rice paddies.


Assuntos
Echinochloa , Herbicidas , Óleos Voláteis , Syzygium , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Echinochloa/efeitos dos fármacos , Syzygium/química , Herbicidas/farmacologia , Herbicidas/química , Cromatografia Gasosa-Espectrometria de Massas
14.
Molecules ; 29(11)2024 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-38893290

RESUMO

Herbicides are useful tools for managing weeds and promoting food production and sustainable agriculture. In this study, we report on the development of a novel class of lipophilic pyrimidine-biphenyl (PMB) herbicides. Firstly, three PMBs, Ia, IIa, and IIIa, were rationally designed via a scaffold hopping strategy and were determined to inhibit acetohydroxyacid synthase (AHAS). Computational simulation was carried out to investigate the molecular basis for the efficiency of PMBs against AHAS. With a rational binding mode, and the highest in vitro as well as in vivo potency, Ia was identified as a preferable hit. Furthermore, these integrated analyses guided the design of eighteen new PMBs, which were synthesized via a one-step Suzuki-Miyaura cross-coupling reaction. These new PMBs, Iba-ic, were more effective in post-emergence control of grass weeds compared with Ia. Interestingly, six of the PMBs displayed 98-100% inhibition in the control of grass weeds at 750 g ai/ha. Remarkably, Ica exhibited ≥ 80% control against grass weeds at 187.5 g ai/ha. Overall, our comprehensive and systematic investigation revealed that a structurally distinct class of lipophilic PMB herbicides, which pair excellent herbicidal activities with new interactions with AHAS, represent a noteworthy development in the pursuit of sustainable weed control solutions.


Assuntos
Herbicidas , Pirimidinas , Herbicidas/química , Herbicidas/farmacologia , Pirimidinas/química , Pirimidinas/farmacologia , Acetolactato Sintase/antagonistas & inibidores , Acetolactato Sintase/metabolismo , Acetolactato Sintase/química , Compostos de Bifenilo/química , Compostos de Bifenilo/antagonistas & inibidores , Simulação de Acoplamento Molecular , Plantas Daninhas/efeitos dos fármacos , Relação Estrutura-Atividade , Estrutura Molecular
15.
Front Public Health ; 12: 1333450, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38894984

RESUMO

Objectives: Diquat poisoning is an important public health and social security agency. This study aimed to develop a prognostic model and evaluate the prognostic value of plasma diquat concentration in patients with acute oral diquat poisoning, focusing on how its impact changes over time after poisoning. Methods: This was a retrospective cohort study using electronic healthcare reports from the Second Hospital of Hebei Medical University. The study sample included 80 patients with acute oral Diquat poisoning who were admitted to the hospital between January 2019 and May 2022. Time-to-event analyses were performed to assess the risk of all-cause mortality (30 days and 90 days), controlling for demographics, comorbidities, vital signs, and other laboratory measurements. The prognostic value of plasma DQ concentration on admission was assessed by computing the area under a time-dependent receiver operating characteristic curve (ROC). Results: Among the 80 patients, 29 (36.25%) patients died, and 51 (63.75%) patients survived in the hospital. Non-survivors had a median survival time (IQR) of 1.3(1.0) days and the longest survival time of 4.5 days after DQ poisoning. Compared with non-survivors, survivors had significantly lower amounts of ingestion, plasma DQ concentration on admission, lungs injury within 24 h after admission, liver injury within 24 h after admission, kidney injury within 24 h after admission, and CNS injury within 36 h after admission, higher APACHE II score and PSS within 24 h after admission (all p < 0.05). Plasma Diquat concentration at admission (HR = Exp (0.032-0.059 × ln (t))) and PSS within 24 h after admission (HR: 4.470, 95%CI: 1.604 ~ 12.452, p = 0.004) were independent prognostic factors in the time-dependent Cox regression model. Conclusion: Plasma DQ concentration at admission and PSS within 24 h after admission are independent prognostic factors for the in-hospital case fatality rate in patients with acute oral DQ poisoning. The prognostic value of plasma DQ concentration decreased with time.


Assuntos
Diquat , Humanos , Estudos Retrospectivos , Masculino , Feminino , Prognóstico , Pessoa de Meia-Idade , Adulto , Diquat/sangue , Herbicidas/sangue , Herbicidas/intoxicação , China
16.
BMC Genomics ; 25(1): 621, 2024 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-38898417

RESUMO

BACKGROUND: Whole plant senescence represents the final stage in the life cycle of annual plants, characterized by the decomposition of aging organs and transfer of nutrients to seeds, thereby ensuring the survival of next generation. However, the transcriptomic profile of vegetative organs during this death process remains to be fully elucidated, especially regarding the distinctions between natural programmed death and artificial sudden death induced by herbicide. RESULTS: Differential genes expression analysis using RNA-seq in leaves and roots of Arabidopsis thaliana revealed that natural senescence commenced in leaves at 45-52 days after planting, followed by roots initiated at 52-60 days. Additionally, both organs exhibited similarities with artificially induced senescence by glyphosate. Transcription factors Rap2.6L and WKRY75 appeared to serve as central mediators of regulatory changes during natural senescence, as indicated by co-expression networks. Furthermore, the upregulation of RRTF1, exclusively observed during natural death, suggested its role as a regulator of jasmonic acid and reactive oxygen species (ROS) responses, potentially triggering nitrogen recycling in leaves, such as the glutamate dehydrogenase (GDH) shunt. Root senescence was characterized by the activation of AMT2;1 and GLN1;3, facilitating ammonium availability for root-to-shoot translocation, likely under the regulation of PDF2.1. CONCLUSIONS: Our study offers valuable insights into the transcriptomic interplay between phytohormones and ROS during whole plant senescence. We observed distinct regulatory networks governing nitrogen utilization in leaf and root senescence processes. Furthermore, the efficient allocation of energy from vegetative organs to seeds emerges as a critical determinant of population sustainability of annual Arabidopsis.


Assuntos
Arabidopsis , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Herbicidas , Senescência Vegetal , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/efeitos dos fármacos , Arabidopsis/metabolismo , Herbicidas/farmacologia , Herbicidas/toxicidade , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Senescência Vegetal/genética , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Folhas de Planta/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/metabolismo , Raízes de Plantas/genética , Transcriptoma , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
17.
Appl Opt ; 63(13): 3712-3724, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38856558

RESUMO

This study aimed to evaluate the effects of herbicide 2, 4-D-dichlorophenoxy acetic acid on golden apple snail eggs and embryos. Additionally, the study assessed the applicability of optical coherence tomography (OCT), a non-invasive depth cross-sectional microscopic imaging technique, as a novel method, to the best of our knowledge, for studying morphological changes in golden apple snail eggs and embryos, in comparison to the conventional approach of using white light microscopy. The study revealed that the herbicide 2,4-D-dichlorophenoxy acetic acid affected the hatchery rate and morphological changes of the eggs and embryos. The lethal concentration (LC50), representing the concentration of a substance that is expected to cause death in half of the population being studied, of the golden apple eggs and embryos increased with longer exposure time and higher concentrations. The estimated median effective concentration (EC50), which denotes the concentration producing the desired effect in 50% of the exposed golden apple embryos, exhibited a similar trend of change as the LC50. When compared to the microscopic study, it was observed that OCT could be employed to investigate morphological changes of golden apple snail eggs and embryos, enabling evaluation of alterations in both 2D and 3D structures.


Assuntos
Ácido 2,4-Diclorofenoxiacético , Embrião não Mamífero , Herbicidas , Tomografia de Coerência Óptica , Animais , Ácido 2,4-Diclorofenoxiacético/farmacologia , Ácido 2,4-Diclorofenoxiacético/toxicidade , Tomografia de Coerência Óptica/métodos , Herbicidas/farmacologia , Herbicidas/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Embrião não Mamífero/embriologia , Caramujos/embriologia , Caramujos/efeitos dos fármacos , Óvulo/efeitos dos fármacos
18.
Sci Total Environ ; 945: 174163, 2024 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-38906309

RESUMO

A novel dual-signal fluorometric and colorimetric probe FMDH (5-FAM-Met-Asp-His-NH2), incorporating a tripeptide (Met-Asp-His-NH2) linked to 5-carboxyfluorescein (5-FAM), was firstly synthesised. FMDH demonstrated exceptional selectivity and sensitivity, rapid response, wide pH response range and robust anti-interference capabilities for monitoring Cu2+. This was achieved through a distinctive naked-eye colorimetric and fluorescent quenching behaviour. A good linearity within the range of 0-3 µM (R2 = 0.9914) was attained, and the limit of detection (LOD) for Cu2+ was 47.4 nM. Furthermore, the FMDH-Cu2+ ensemble responded to glyphosate with notable selectivity and sensitivity. A good linear correlation (R2 = 0.9926) was observed at the lower concentration range (2.4-7.8 µM) and achieving a detection limit as low as 29.9 nM. The response time of FMDH with Cu2+ and glyphosate were less than 20 s, and the pH range of 7-11 that was suitable for practical application under physiological pH conditions. MTT assays confirmed that FMDH offers good permeability and low toxicity, facilitating successful application in imaging analysis of Cu2+ and glyphosate in living cells and zebrafish. In addition, FMDH was employed in the detection of these analytes in real water samples. Cost-effective, highly sensitive and easily prepared FMDH-impregnated test strips were developed for the efficient visual detection of Cu2+ and glyphosate under 365 nm UV light. Increasing concentrations of Cu2+ and glyphosate resulted in notable colour changes under 365 nm UV light, enabling visual semi-quantitative analysis via a smartphone colour-analysis App.


Assuntos
Colorimetria , Cobre , Fluorometria , Glicina , Glifosato , Poluentes Químicos da Água , Cobre/análise , Glicina/análogos & derivados , Glicina/análise , Colorimetria/métodos , Poluentes Químicos da Água/análise , Fluorometria/métodos , Corantes Fluorescentes/química , Herbicidas/análise , Limite de Detecção , Peptídeos , Monitoramento Ambiental/métodos , Animais
19.
Sci Rep ; 14(1): 13327, 2024 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-38858445

RESUMO

This study investigates the environmental impact of burning herbicide-contaminated biomass, focusing on atrazine (ATZ) and diuron (DIU) sprayed on rice straw prior to burning. Samples of soil, biomass residues, total suspended particulate (TSP), particulate matter with an aerodynamic diameter ≤ 10 µm (PM10), and aerosols were collected and analyzed. Soil analysis before and after burning contaminated biomass showed significant changes, with 2,4-dichlorophenoxyacetic acid (2,4-D) initially constituting 79.2% and decreasing by 3.3 times post-burning. Atrazine-desethyl, sebuthylazine, and terbuthylazine were detected post-burning. In raw rice straw biomass, terbuthylazine dominated at 80.0%, but burning ATZ-contaminated biomass led to the detection of atrazine-desethyl and notable increases in sebuthylazine and terbuthylazine. Conversely, burning DIU-contaminated biomass resulted in a shift to 2,4-D dominance. Analysis of atmospheric components showed changes in TSP, PM10, and aerosol samples. Linuron in ambient TSP decreased by 1.6 times after burning ATZ-contaminated biomass, while atrazine increased by 2.9 times. Carcinogenic polycyclic aromatic hydrocarbons (PAHs), including benzo[a]anthracene (BaA), benzo[a]pyrene (BaP), and benzo[b]fluoranthene (BbF), increased by approximately 9.9 to 13.9 times after burning ATZ-contaminated biomass. In PM10, BaA and BaP concentrations increased by approximately 11.4 and 19.0 times, respectively, after burning ATZ-contaminated biomass. This study sheds light on the environmental risks posed by burning herbicide-contaminated biomass, emphasizing the need for sustainable agricultural practices and effective waste management. The findings underscore the importance of regulatory measures to mitigate environmental contamination and protect human health.


Assuntos
Atrazina , Biomassa , Diurona , Herbicidas , Oryza , Solo , Atrazina/análise , Oryza/química , Herbicidas/análise , Solo/química , Diurona/análise , Poluentes do Solo/análise , Poluentes Atmosféricos/análise , Monitoramento Ambiental , Material Particulado/análise
20.
Biosens Bioelectron ; 261: 116487, 2024 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-38870829

RESUMO

A new di-recognition nitrogen-doped carbon dot nanosurface aptamer molecularly imprinted polymer (CDNAg@MIPApt) nanocatalytic di-functional probe was prepared by microwave irradiation. The probe was utilized nitrogen-doped silver carbon dots (CDNAg) as the matrix, glyphosate (Gly) as the template molecule, α-methyl acrylate as the monomer, ethylene glycol dimethacrylate as the cross-linker, and aptamer as the biorecognition element. It could not only recognize Gly but also exhibits catalytic amplification function. It was found that CDNAg@MIPApt catalyzed the redox reaction of polyethylene glycol 400 (PEG400)-AgNO3 to generate silver nanoparticles (AgNPs). The AgNPs indicator component exhibit the effects of surface-enhanced Raman scattering (SERS), resonance Rayleigh scattering (RRS) and surface plasmon resonance absorption (Abs). In the presence of Gly, it binds to the surface imprinted site of CDNAg@MIPApt, to reduce AgNPs generation due to the catalytic activity of CDNAg@MIPApt decreasing. Thus, the SERS/RRS/Abs signal values decreased linearly. The linear ranges of SERS/RRS/Abs assay were 0.1-2.5 nM, 0.25-2.75 nM and 0.5-5 nM respectively. The detection limits were 0.034 nM, 0.071 nM and 0.18 nM Gly.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Glicina , Glifosato , Limite de Detecção , Nanopartículas Metálicas , Polímeros Molecularmente Impressos , Prata , Análise Espectral Raman , Glicina/química , Glicina/análogos & derivados , Prata/química , Polímeros Molecularmente Impressos/química , Aptâmeros de Nucleotídeos/química , Nanopartículas Metálicas/química , Técnicas Biossensoriais/métodos , Ressonância de Plasmônio de Superfície/métodos , Herbicidas/análise , Herbicidas/química , Carbono/química
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