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1.
Anim Sci J ; 91(1): e13395, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32484296

RESUMO

This study aimed to investigate the effects of whey protein hydrolysate (WPH) on the growth and immunity of mouse pups in artificial rearing (AR) system. Mouse pups were reared in the AR system with artificial milk including 5% WPH (AR with WPH) or not (AR without WPH), and the remaining pups were reared by their mother (dam) for 14 days after birth. The body weight change and body weight gain rates in the AR with WPH group were significantly higher than those observed in the AR without WPH group and similar to those in the dam group. Moreover the feed and protein efficiencies in the AR with WPH group were significantly higher than those of the AR without WPH group. In addition, the supplement of WPH in the AR system was shown to significantly elevate the number of CD3+ CD8+ , B220+ CD19+ , IA/IE+ CD11c+ , and CD11b+ in the thymocyte and/or splenocyte, and the thymus weight. Furthermore, MALDI-TOF/MS analysis identified the amino acid sequences corresponding to some peptides, and indicated that VRTPEVDDE had the highest relative intensity among the peptides from tested WPH. Therefore, WPH would be required to not only promote growth, but also exert immunomodulatory activities in mouse pups in AR system.


Assuntos
Ração Animal , Criação de Animais Domésticos/métodos , Fenômenos Fisiológicos da Nutrição Animal/efeitos dos fármacos , Dieta/veterinária , Imunomodulação/efeitos dos fármacos , Camundongos/crescimento & desenvolvimento , Camundongos/imunologia , Hidrolisados de Proteína/farmacologia , Proteínas do Soro do Leite , Animais , Antígenos CD/metabolismo , Suplementos Nutricionais , Hidrolisados de Proteína/administração & dosagem , Baço/metabolismo , Timócitos/metabolismo
2.
J Food Sci ; 85(6): 1735-1741, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32468582

RESUMO

In this study, the antioxidant activity of mungbean protein hydrolysate (MPH) was systematically investigated. MPH was fractionated by ultrafiltration into two major fractions (MPH-1 <3 kDa, MPH-2 >3 kDa). Fraction MPH-1, which exhibited the highest antioxidant activity, was further fractionated by gel column into three fractions (MPH-1A, MPH-1B, and MPH-1C). The antioxidant activity of the MPH-1B fraction was stronger than that of the other fractions. Eight mungbean peptides (P1-P8) were identified in fraction MPH-1B by UPLC-Q-TOF-MS. Among them, peptides Trp-Gly-Asn (WGN, P2), Ala-Trp (AW, P4), Arg-Gly-Trp-Tyr-Glu (RGWYE, P5), and Gly-Val-Pro-Phe-Trp (GVPFW, P7) had high antioxidant activity. Moreover, these four peptides exerted protective effects against H2 O2 -induced cytotoxicity and regulated the MDA content, CAT activity, and total GSH content in HepG2 cells with specific observation. This study demonstrated the potential of MPH as a source of antioxidant peptides. This provides a scientific basis for the preparation of antioxidant peptides from mungbean protein. PRACTICAL APPLICATION: This study demonstrated the potential of the hydrolysate of mungbean protein as a source of antioxidant peptides and provided a scientific basis for the preparation of antioxidant peptides from mungbean protein.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Proteínas de Plantas/química , Vigna/química , Sequência de Aminoácidos , Animais , Antioxidantes/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Células Hep G2 , Humanos , Peptídeos/química , Proteínas de Plantas/farmacologia , Hidrolisados de Proteína/química , Hidrolisados de Proteína/farmacologia
3.
Food Chem ; 328: 127135, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32473490

RESUMO

Watermelon seed, a watermelon processing industry by-product, is a good protein source for the preparation of antioxidant peptides due to its high protein content, low cost, special amino acid composition. Antioxidant hydrolysates obtained from watermelon seed protein (WSP) after slit divergent ultrasound (SDU) treatment were studied. The stepwise multiple linear regression model verified that the reducing power of watermelon seed protein hydrolysates (WSPHs) is positively related with -SH and ß-turn content of WSP (R2 = 0.931, p < 0.01). Using the degree of hydrolysis (DH) and reducing power as indicators, the WSPHs was prepared under the optimal conditions (ultrasound frequency: 20/28 kHz, time: 60 min, power density: 100 W/L) and divided into three components by ultrafiltration membrane (1 and 5 kDa). Compared with WSPHs and other fractions, WSPHs-I (Mw < 1 kDa) not only significantly protected HepG2 cells from H2O2-induced damage, but also greatly alleviated the liver injury caused by d-galactose in male SD rats.


Assuntos
Antioxidantes/farmacologia , Citrullus/química , Hidrolisados de Proteína/química , Hidrolisados de Proteína/farmacologia , Animais , Antioxidantes/química , Antioxidantes/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Enzimas/metabolismo , Galactose/toxicidade , Células Hep G2 , Humanos , Peróxido de Hidrogênio/farmacologia , Hidrólise , Modelos Lineares , Masculino , Proteínas de Plantas/química , Proteínas de Plantas/farmacologia , Ratos Sprague-Dawley , Sementes/química , Ultrassom/instrumentação , Ultrassom/métodos
4.
Food Chem ; 319: 126563, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32172048

RESUMO

Enzymatic hydrolysis of proteins produces bioactive peptides that have the potential to provide health benefits. This study examined the inflammatory- and immune-modulating properties of a flavourzyme-derived sunflower protein hydrolysate (SPH) and peptides. The SPH was fractionated into <1, 1-3, 3-5, and >5 kDa peptides by membrane ultrafiltration. The SPH blunted IL-1ß stimulated NFκB activation and boosted IL-4/GM-CSF induced expression of surface markers CD14 and CD86, indicating maturation into a dendritic cell (DC) phenotype. Testing of SPH membrane ultrafiltration and HPLC fractions indicated that smaller and non-polar peptides were the most potent, respectively. Four novel peptides (YFVP, SGRDP, MVWGP and TGSYTEGWS) were identified and all of them blunted IL-1ß stimulated NFκB activation. The peptides also boosted IL-4/GM-CSF induction of CD14, while only MVWGP and TGSYTEGWS boosted the expression of CD86. MVWGP was the most potent immune-modulatory peptide across all cellular assays, which was attributed to the presence of a methionine residue.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Células Dendríticas/efeitos dos fármacos , Helianthus/metabolismo , Monócitos/efeitos dos fármacos , NF-kappa B/antagonistas & inibidores , Peptídeos/farmacologia , Hidrolisados de Proteína/farmacologia , Linhagem Celular Tumoral , Células Dendríticas/citologia , Células Dendríticas/metabolismo , Humanos , Monócitos/citologia , Monócitos/metabolismo , NF-kappa B/metabolismo , Peptídeos/metabolismo , Hidrolisados de Proteína/metabolismo
5.
Poult Sci ; 99(3): 1693-1704, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32111333

RESUMO

This study examined the antioxidant capabilities of peptides derived from chicken feather meal (CFM) protein hydrolysates which were produced using 3 different microbial proteases (Neutrase, Alcalase, and flavourzyme) and tested at varying concentrations, namely 1, 2, and 5% by weight. The highest levels of 2,2-diphenyl-1-picrylhydrazl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities were presented by CFM hydrolysate derived using 5 wt% Neutrase and digested for 4 h. Fractionation of this particular hydrolysate was then performed by applying 10, 5, 3, and 0.65 kDa molecular weight cutoff membranes. It was then determined that the molecular weight (MW) < 0.65 kDa fraction achieved the greatest level of free radical scavenging activity in the context of DPPH and ABTS. The MW < 0.65 kDa fraction then underwent additional fractionation using reverse-phase high-performance liquid chromatography to derive 3 main fractions designated as F1, F2, and F3. All of these fractions presented a high level of activity in DPPH radical scavenging, although no significant ABTS scavenging was observed. Quadrupole time-of-flight tandem mass spectrometry was used in determining the peptide contents of the fractions as Phe-Asp-Asp-Arg-Gly-Arg-X for F1 (FDDRGRX, 875 Da), Val-Thr-Leu-Ala-Val-Thr-Lys-His for F2 (VTLAVTKH, 868 Da), and Val-Ser-Glu-Ile-X-Ser-Ile-Pro-Ile-Ser for F3 (VSEIXSIPIS, 1,055 Da). Moreover, the F2 fraction was shown to be capable of preventing DNA damage induced by hydroxyl radicals, as indicated in tests using the plasmids pKS, pUC19, and pBR322 via the Fenton reaction. This outcome was demonstrated through in vitro antiproliferative activity in human cell lines based on SW620 colon cancer, using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. The F2 fraction at 0.5 wt.% was also shown to be capable of inducing weak early apoptosis, which could be measured by using the Fluorescein isothiocyanate Annexin V Apoptosis Detection Kit with Propidium Iodide Solution. Furthermore, an increase in caspase-3 and caspase-8 activity was observed in SW620 cells following exposure for 24 h and 48 h.


Assuntos
Plumas/química , Radicais Livres/antagonistas & inibidores , Hidrolisados de Proteína/química , Hidrolisados de Proteína/farmacologia , Animais , Antioxidantes , Apoptose , Linhagem Celular Tumoral , Galinhas , Humanos , Peptídeos/química
6.
J Pharmacol Sci ; 143(1): 52-55, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32115365

RESUMO

Recently, we has reported that AMPK activator has antidepressant effect. Previous our study suggested that liver hydrolysate (LH) activated adenosine monophosphate-activated protein kinase (AMPK) in periphery. However, the effect of LH on depression is unclear. Therefore, we examines whether LH has antidepressant effect on olfactory bulbectomized (OBX) mice. OBX mice showed depressive-like behavior in tail-suspension test and reduction of hippocampal neurogenesis, while these changes were reversed by LH. LH enhanced hippocampal phosphate-AMPK, brain-derived neurotrophic factor (BDNF) and phosphate-cyclic adenosine monophosphate response element-binding protein (CREB) in OBX mice. These data indicate that LH may produce antidepressant effects via hippocampal AMPK/BDNF/CREB signaling.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Transtorno Depressivo/tratamento farmacológico , Transtorno Depressivo/etiologia , Hipocampo/fisiologia , Neurogênese , Bulbo Olfatório/fisiologia , Bulbo Olfatório/cirurgia , Hidrolisados de Proteína/farmacologia , Hidrolisados de Proteína/uso terapêutico , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Animais , Transtorno Depressivo/genética , Modelos Animais de Doenças , Masculino , Camundongos Endogâmicos
7.
J Dairy Sci ; 103(3): 2053-2064, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31882211

RESUMO

We evaluated the acute (single-dose) and subacute (repeated-dose) oral toxicity of alcalase-hydrolyzed whey protein concentrate. Our acute study revealed no death or treatment-related complications, and the median lethal dose of whey protein concentrate hydrolysate was >2,500 mg/kg. In the subacute study, when the hydrolysate was fed at 3 different concentrations (200, 400, and 800 mg/kg), no groups showed toxicity changes compared with controls. Then, whey protein concentrate hydrolysate was orally administered to spontaneously hypertensive rats. Results revealed significant reductions in blood pressure in a dose-dependent manner, and dosing at 400 mg/kg led to significant blood pressure reduction (-47.8 mm Hg) compared with controls (blood pressure maintained) and the findings of previous work (-21 mm Hg). Eight peptides-RHPEYAVSVLLR, GGAPPAGRL, GPPLPRL, ELKPTPEGDL, VLSELPEP, DAQSAPLRVY, RDMPIQAF, and LEQVLPRD-were sequentially identified and characterized. Of the peptides, VLSELPEP and LEQVLPRD showed the most prominent in vitro angiotensin-I converting enzyme inhibition with half-maximal inhibitory concentrations of 0.049 and 0.043 mM, respectively. These findings establish strong evidence for the in vitro and in vivo potential of whey protein concentrate hydrolysate to act as a safe, natural functional food ingredient that exerts antihypertensive activity.


Assuntos
Anti-Hipertensivos/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Proteínas do Soro do Leite/farmacologia , Inibidores da Enzima Conversora de Angiotensina/química , Animais , Anti-Hipertensivos/química , Anti-Hipertensivos/toxicidade , Feminino , Hidrólise , Masculino , Peptídeos/farmacologia , Hidrolisados de Proteína/química , Hidrolisados de Proteína/farmacologia , Hidrolisados de Proteína/toxicidade , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Subtilisinas/metabolismo , Proteínas do Soro do Leite/química , Proteínas do Soro do Leite/toxicidade
8.
J Sci Food Agric ; 100(1): 59-73, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31435933

RESUMO

BACKGROUND: Jiuzao is the residue after Bajiu distillation which is usually used as forage for livestock. However, it is not fully utilized yet considering the content of protein remained. The present study aimed to isolate antioxidant peptides from Jiuzao protein hydrolysates, then add these peptides into Baijiu product to enhance the healthy value of Baiju. Meanwhile environmental pollution caused by massive Jiuzao can be mitigated indirectly. RESULTS: Four peptides Ala-Tyr-Ile(Leu) (AYI(L)) and Asp-Arg-Glu-Ile(Leu) (DREI(L)) were identified from Jiuzao protein hydrolysates, the extraction contents of AYI + AYL and DREI + DREL were 896.10 and 110.51 mg kg-1 Jiuzao, respectively. On the one hand, antioxidant activities of these peptides were investigated. For in vitro antioxidant assays, AYI, AYL and DREI exhibited strong capacities in oxygen radical absorbance capacity (ORAC) assay. Furthermore, three levels of four peptides were assessed by 2,2'-azobis(2-methylpropanimidamidine) (AAPH)-induced HepG2 cells model. The results showed that these peptides exerted a degree of antioxidant activities in cells. Meanwhile, selected peptides concentrations according to cell assays remined at effective doses after in vitro digestion. On the other hand, the influence of these four peptides on the characteristic aroma compounds in Baijiu was studied. Most characteristic aroma compounds releases were increased with the addition of peptides. CONCLUSION: In the study, antioxidant activities of peptides were evaluated, the feasibility of utilizing Jiuzao protein hydrolysates to obtain beneficial peptides was also proved. Healthy effect of Baijiu or other food can be increased by adding these functional substances. The findings might contribute to food application and Baijiu industries. © 2019 Society of Chemical Industry.


Assuntos
Antioxidantes/química , Peptídeos/química , Hidrolisados de Proteína/química , Resíduos/análise , Vinho/análise , Antioxidantes/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Hep G2 , Humanos , Peptídeos/farmacologia , Hidrolisados de Proteína/farmacologia
9.
Carbohydr Polym ; 229: 115472, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31826419

RESUMO

TEMPO oxidized cellulose nanofibers (T-CNF) were prepared from cellulose pulp which is extracted from bagasse. Soy protein hydrolysate (SPH) was grafted on T-CNF via amidation of carboxylic groups. Biomineralization was, then, assessed via calcium phosphates (CaP) precipitation in twice-simulated body fluid until formation of a new bioactive material. Protein was efficiently grafted without alteration of morphology and nanofibrils packing as reported by Fourier Transform infrared analysis /X Ray Diffraction /Scanning and Transmission Electron Microscopy / Atomic Force Microscopy. Highly crystalline calcium phosphate deposits - ca. 22.1% - were detected, with a Ca/P ratio equal to 1.63, in agreement with native bone apatite composition. In vitro response of human Mesenchymal Stem Cells confirmed the biocompatibility. No significant differences in terms of cell adhesion were recognized while a significant increase in cell proliferation was detected until 7 days. The presence of calcium phosphates tends to cover the nanofibrillar pattern, inducing the inhibition of cell proliferation and promoting the ex-novo precipitation of mineral phases. All the results suggest a promising use of these biomaterials in the repair and/or the regeneration of hard tissues such as bone.


Assuntos
Materiais Biocompatíveis/farmacologia , Calcificação Fisiológica/efeitos dos fármacos , Celulose/farmacologia , Nanofibras/química , Hidrolisados de Proteína/farmacologia , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/toxicidade , Fosfatos de Cálcio/síntese química , Fosfatos de Cálcio/farmacologia , Fosfatos de Cálcio/toxicidade , Proliferação de Células/efeitos dos fármacos , Celulose/análogos & derivados , Celulose/toxicidade , Óxidos N-Cíclicos/química , Géis/síntese química , Géis/farmacologia , Géis/toxicidade , Humanos , Nanocompostos/química , Nanocompostos/toxicidade , Nanofibras/toxicidade , Oxirredução , Hidrolisados de Proteína/química , Hidrolisados de Proteína/toxicidade , Soja/química
10.
J Food Sci ; 84(12): 3330-3340, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31834967

RESUMO

There is a growing demand in reusing agro-industrial waste to enrich food proteins and develop new protein-related products. Brewer's spent grain (BSG), the byproducts generated from the processing of beer-brewing industry, is rich in abundant protein (26 to 30%) with good physicochemical properties and nutritional benefits. BSG is mainly composed of four proteins including hordein, gluten, globulin, and albumin. The methods for extracting protein from BSG mainly include alkali extraction, ultrasonic-assisted extraction, and organic solvent extraction, among others. However, little researches have described the functional properties of Brewer's spent grain protein (BSGP) and how it can be improved by enzymatic modification. Additionally, BSG protein hydrolysates (BSGPHs) have good bioactivities, mainly including antioxidant, antiinflammatory, and angiotensin-I-converting enzyme inhibitory activities, among others. Based on the above situation, this review provides a comprehensive overview about the isolation, physicochemical features, application of the BSGP. In addition, the functional properties and biological activities of BSGPHs are also emphasized. The purpose of this review is to provide an up-to-date summary of BSGP research, and to broaden the market potential of this emerging protein in food industry. Besides, this review provides a reference to study the use of BSGP or hydrolysates for a variety of purposes in-depth, including pharmaceutical, food, and industrial applications.


Assuntos
Grão Comestível/química , Proteínas de Grãos/química , Hidrolisados de Proteína/química , Resíduos/análise , Animais , Cerveja/análise , Indústria Alimentícia , Proteínas de Grãos/isolamento & purificação , Proteínas de Grãos/farmacologia , Humanos , Resíduos Industriais/análise , Hidrolisados de Proteína/isolamento & purificação , Hidrolisados de Proteína/farmacologia
11.
Mar Drugs ; 17(12)2019 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-31817914

RESUMO

BACKGROUND: Krill (Euphausia superba) represent the largest animal biomass on earth, and are a rich source of high-quality protein with essential amino acids. Krill-derived peptides are renowned for their antioxidant activities. Hence, these peptides may have protective effects against oxidative stress. Alcoholic liver disease is a prevalent cause of death worldwide. The present study explores the hepatoprotective effects of krill peptide hydrolysate fractions against ethanol-induced liver damage in BALB/c mice. METHODS: Hydrolysis was carried out by mimicking the gastrointestinal digestion environment and the filtrate was fractionated based on molecular weight (<1 kDa, 1-3 kDa, and >3 kDa). The 1-3 kDa fraction (KPF), which indicated the highest antioxidant effect, was further investigated for its effect on weight and survival rate increase in mice and its influence on serum glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and liver cholesterol levels. Moreover, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels were measured, followed by Nrf2 and HO-1 expression. Histopathology studies were conducted to assess hepatic tissue damage. RESULTS: KPF enhanced the weight and survival rate of mice while reducing serum glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and liver cholesterol levels. Moreover, KPF upregulated SOD, CAT, and GPx in liver tissues, while downregulating tumor necrosis factor α and interleukin-6 mRNA expression. KPF further increased Nrf2 and HO-1 expression and suppressed ethanol-induced apoptotic proteins in the liver. Histopathology of KPF-treated mice showed less hepatic tissue damage compared to ethanol-treated mice. CONCLUSIONS: Hydrolysates and bioactive peptides prepared from krill can be employed as functional foods to enhance liver function and health. Further investigations of KPF could lead to the development of functional foods.


Assuntos
Euphausiacea/química , Hepatopatias Alcoólicas/prevenção & controle , Estresse Oxidativo/efeitos dos fármacos , Hidrolisados de Proteína/farmacologia , Animais , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Modelos Animais de Doenças , Etanol/toxicidade , Alimento Funcional , Fígado/efeitos dos fármacos , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Peptídeos/isolamento & purificação , Peptídeos/farmacologia , Hidrolisados de Proteína/isolamento & purificação
12.
Vopr Pitan ; 88(4): 18-24, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31722137

RESUMO

Among various food proteins, soybean proteins have the greatest traditions of application for the dietary correction and prevention of lipid metabolism disorders and related complications. Aim. In an in vivo experiment using male Wistar rats, the lipid-lowering properties of soy protein and its enzymatic hydrolysate were tested to evaluate their possible use as ingredients of specialized foods. Material and methods. Animals were randomly divided into 3 groups: control group G1 and 2 experimental groups G2 and G3. The total duration of the experiment was 70 days. The animals of the control group G1 were fed with high-lipid semi-synthetic diet. Animals of the experimental groups G2 and G3 received the same high-fat semi-synthetic diet, but with a 50% replacement of casein with soy protein isolate (SPI) and enzymatic hydrolyzate of SPI (EHSPI), respectively. The blood glucose was measured once per 2 weeks. At the end of the experiment on the 71st day the level of glycated hemoglobin was determined in the blood; the levels of triglycerides, cholesterol, high density lipoproteins (HDL), low density lipoproteins (LDL) and the concentration of malon dialdehyde were determined in the serum. Results and discussion. Starting from the 6th week of the experiment and prior to its completion, the average food intake of animals from the G3 group was significantly (р<0.05) lower compared to animals of the G1 control group. The food intake of animals of group G2 was significantly (р<0.05) reduced compared with this indicator for animals of group G1, starting from the week 8 of the experiment and prior to its completion. The monitoring of the body weight gain did not reveal significant differences between all groups of animals, despite differences in the food intake. Replacing casein in the diet by 50% with SPI had a pronounced antioxidant and cholesterol-lowering effect. The total cholesterol content (1.65±0.05 mmol/l) decreased significantly (р<0.05) due to a decrease in LDL (0.90±0.03 mmol/l), and malon dialdehyde level lowered (3.7±0.5 µmol/l, р<0.05) in the serum of group G2 rats compared with animals of the control group G1 (2.01±0.13 and 1.12±0.09 mmol/l; 5.1±0.4 µmol/l, respectively). Replacing casein by 50% with EHSPI in the diet of G3 rats was unfavorable, significantly (р<0.05) increasing the level of total cholesterol (2.76±0.16 mmol/l) and cholesterol in LDL (1.66±0.12 mmol/l) in blood of these animals compared with animals of both comparison groups G1 and G2. Conclusion. A preclinical comparative study of the cholesterol-lowering and antioxidant properties of the SPI substantiates the prospect of its following clinical trials with the aim of including into the composition of specialized foods for prevention and diet therapy of the disorders of endogenous cholesterol homeostasis.


Assuntos
Gorduras na Dieta/efeitos adversos , Síndrome Metabólica , Obesidade , Hidrolisados de Proteína/farmacologia , Proteínas de Soja/farmacologia , Animais , Gorduras na Dieta/farmacologia , Masculino , Síndrome Metabólica/induzido quimicamente , Síndrome Metabólica/dietoterapia , Síndrome Metabólica/metabolismo , Síndrome Metabólica/patologia , Obesidade/induzido quimicamente , Obesidade/dietoterapia , Obesidade/metabolismo , Obesidade/patologia , Ratos , Ratos Wistar
13.
Drug Discov Ther ; 13(5): 244-247, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31611487

RESUMO

Silkworms are useful for evaluating substances that suppress postprandial hyperglycemia by oral administration. In this study, orally administered whey protein hydrolysate (WPH), obtained by enzymatic treatment of whey protein, suppressed sucrose-induced hyperglycemia in silkworms in a dose-dependent manner. WPH also inhibited glucose-induced hyperglycemia in silkworms. These findings suggest that WPH contains a bioactive peptide that inhibits glucose uptake from the intestinal tract and thereby suppresses sucrose-induced hyperglycemia.


Assuntos
Hiperglicemia/prevenção & controle , Hidrolisados de Proteína/farmacologia , Proteínas do Soro do Leite/farmacologia , Animais , Bombyx , Relação Dose-Resposta a Droga , Glucose , Hiperglicemia/induzido quimicamente , Sacarose
14.
Food Funct ; 10(10): 6792-6802, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31577280

RESUMO

Skin photoaging induced by consecutive exposure of skin to ultraviolet radiation is primarily responsible for skin aging and preparation of food-derived ingredients with anti-aging functions has been the hot topic worldwide. Dietary consumption of food supplements has been found to modulate skin functions and can be useful in the prevention of skin aging. To evaluate the effect of walnut protein hydrolysate (WPH) on photoaged skin, Sprague-Dawley rats (SD rats) were orally administered with WPH and then were regularly exposed to ultraviolet radiation (UV-R). After a consecutive UV-R for 18 weeks, the delaying efficiency of WPH against elasticity degradation was examined and the mechanical mechanism was explored subsequently. The contents of hydroxyproline (Hyp) and hyaluronic acid (HA) in the extracellular matrix (ECM) were measured by biochemical reactions and color rendering procedures; the levels of types I and III collagen (Col I and III) and the activity of matrix metalloproteinase-1 (MMP-1) were detected by enzyme-linked immunosorbent assay (ELISA); the protein levels of elastin and fibrillin-1 were examined by western blotting. Moreover, the histological change in the skin structure was illustrated by hematoxylin & eosin (HE) and Masson staining. The results revealed that WPH evidently enhanced the elasticity of photoaged skin and stimulated the biosynthesis of ECM components Col I, Hyp and HA in the dermal layer; meanwhile WPH inhibited the MMP-1 activity, alleviated epidermal hyperplasia, and repaired the damaged skin mechanical structure in a dose-dependent manner. In particular, in comparison with the UV-R group, the WPH group in which WPH was administered at a high-dose level showed significantly improved skin appearance, ECM components and structure (P < 0.05). Taken together, the elasticity improvement caused by WPH against the skin photoaging process can be attributed to the regulation of the metabolism of the components and repair of the damaged mechanical structure of the ECM. This research proved the potential of WPH as a functional ingredient for the development of anti-photoaging foods.


Assuntos
Juglans/química , Hidrolisados de Proteína/farmacologia , Envelhecimento da Pele/efeitos dos fármacos , Envelhecimento da Pele/efeitos da radiação , Pele/efeitos dos fármacos , Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Animais , Colágeno/metabolismo , Dieta , Suplementos Nutricionais , Elasticidade , Elastina/efeitos dos fármacos , Feminino , Fibrilina-1/metabolismo , Ácido Hialurônico/farmacologia , Hidroxiprolina , Masculino , Metaloproteinase 1 da Matriz/metabolismo , Modelos Animais , Ratos , Ratos Sprague-Dawley , Pele/patologia , Envelhecimento da Pele/patologia
15.
Food Funct ; 10(10): 6732-6739, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31576391

RESUMO

Hemp (Cannabis sativa L.) seeds are well known for their potential use as a source of nutrients, fiber, and bioactive compounds. A hemp protein isolate, prepared from defatted hemp flour, was hydrolyzed by alcalase and flavourzyme under specific conditions. The resulting hydrolysates were evaluated for the selection of potentially bioactive hemp protein hydrolysates (HPHs) owing to their DPPH scavenging and ferric reducing antioxidant power activity. In vitro cell-free experiments led to the identification of two bioactive HPHs, HPH20A and HPH60A + 15AF, which were used at 50 and 100 µg mL-1 on BV-2 microglial cells in order to evaluate the anti-neuroinflammatory activities. Our results showed that HPH20A and HPH60A + 15AF down-regulated TNF-α, IL-1ß, and IL-6 mRNA transcriptional levels in LPS-stimulated BV-2 microglial cells. In addition, HPH20A and HPH60A + 15AF up-regulated the gene expression of anti-inflammatory cytokine IL-10. This study suggests for the first time that HPHs may improve the neuroinflammatory and inflammatory states, supporting the nutraceutical value of hemp seeds.


Assuntos
Antioxidantes/farmacologia , Cannabis/química , Fármacos Neuroprotetores/farmacologia , Hidrolisados de Proteína/farmacologia , Sementes/química , Aminoácidos/análise , Animais , Antioxidantes/química , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Suplementos Nutricionais/análise , Endopeptidases/metabolismo , Farinha/análise , Regulação da Expressão Gênica , Hidrólise , Interleucina-10/genética , Interleucina-10/metabolismo , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Interleucina-6/genética , Interleucina-6/metabolismo , Camundongos , Microglia/citologia , Microglia/metabolismo , Fármacos Neuroprotetores/química , Proteínas de Plantas/química , Proteínas de Plantas/farmacologia , Hidrolisados de Proteína/química , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Subtilisinas/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
16.
J Dairy Sci ; 102(12): 10711-10723, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31548055

RESUMO

The objective of this work was to obtain casein hydrolysates with aspartic proteinases present in extracts from the artichoke flower (Cynara scolymus L.) and evaluate their antioxidant, antimicrobial, and angiotensin-I converting enzyme (ACE) inhibitory activity in vitro. The casein hydrolysates produced by the action of C. scolymus had elevated antihypertensive and antioxidant activity due to their high hydrophobic peptide content (93.84, 96.58, and 90.54% at 2, 4, and 16 h of hydrolysis, respectively). Hydrolysis time and molecular weight (<3 kDa) had a significant influence on the hypertensive and antioxidant activity of the hydrolysates, which were greater at hydrolysis times of 4 and 16 h and corresponding to the <3 kDa fractions. The <3 kDa fraction of the 16 h hydrolysate had an ACE inhibitory activity with a half-maximal inhibitory concentration (IC50) of 71.77 µg peptides per mL; DPPH and ABTS•+ radical scavenging activities of 6.27 µM and 6.21 mM Trolox equivalents per mg of peptides, respectively; and iron (II) chelation activity with an IC50 of 221.49 µg of peptides per mL. Antimicrobial activity against Enterococcus faecalis was also observed in the hydrolysates. From the peptide sequences identified in the hydrolysates, we detected 22 peptides (from the BIOPEP database) that were already in their bioactive form (AMKPWIQPK, AMKPWIQPKTKVIPYVRYL, ARHPHPHLSFM, DAQSAPLRVY, FFVAPFPEVFGK, GPVRGPFPII, KVLPVPQK, LLYQEPVLGPVRGPFPIIV, MAIPPKKNQDK, NLHLPLPLL, PAAVRSPAQILQ, RELEELNVPGEIVESLSSSEESITR, RPKHPIKHQ, RPKHPIKHQGLPQEVLNENLLRF, SDIPNPIGSENSEK, TPVVVPPFLQP, VENLHLPLPLL, VKEAMAPK, VLNENLLR, VYPFPGPIH, VYQHQKAMKPWIQPKTKVIPYVRY, VYQHQKAMKPWIQPKTKVIPYVRYL) and are reported to display antioxidant, antimicrobial, and ACE inhibitory activity. We also identified 12,116, 14,513, and 25,169 peptide sequences in the hydrolysates at 2, 4, and 16 h, respectively, that were contained in the primary sequence, and these are reported to display ACE inhibitory, antioxidant, dipeptidyl peptidase IV inhibition, antithrombotic, opioid, immunomodulation, antiamnesic, anticancer, chelating, and hemolytic bioactivity.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/farmacologia , Anti-Infecciosos/farmacologia , Antioxidantes/farmacologia , Caseínas/farmacologia , Cynara scolymus/enzimologia , Peptídeo Hidrolases/metabolismo , Hidrolisados de Proteína/farmacologia , Inibidores da Enzima Conversora de Angiotensina/química , Inibidores da Enzima Conversora de Angiotensina/isolamento & purificação , Animais , Anti-Infecciosos/isolamento & purificação , Anti-Hipertensivos/isolamento & purificação , Anti-Hipertensivos/farmacologia , Antioxidantes/química , Caseínas/isolamento & purificação , Bovinos , Peso Molecular , Hidrolisados de Proteína/química , Hidrolisados de Proteína/isolamento & purificação
17.
Mol Biol Rep ; 46(6): 6079-6085, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31493283

RESUMO

Food proteins from different sources can provide beneficial effects on human health by releasing the bioactive peptides that are integral part of their native structure. In this study, we tested the biological potential of hempseed protein hydrolysates (HPHs) obtained from hempseed cake protein isolate. The HPHs were prepared by enzyme hydrolysis using three different proteases of microbial origin: Alcalase®, Neutrase® and Protamex®. The antioxidant activity of the obtained hydrolysates was determined by oxygen radical absorbance capacity (ORAC) assay, while the proliferative effects on normal (HaCaT) and cancer (HeLa) cells were determined by the CellTiter 96® AQueous One Solution Reagent (MTS) assay. HPHs showed dose-dependent antiproliferative effects on HeLa cells and stimulatory effects on the proliferation of HaCaT cells. HPH obtained by Neutrase® (HPH-N) showed the highest antioxidant activity expressed as an ORAC value. The protective effect of HPH-N on H2O2-induced oxidative stress in normal and cancer cells was evaluated and 1 mg/mL of HPH-N significantly reduced the formation of intracellular reactive oxygen species (ROS) in both cell lines. The obtained results indicate the benefits of HPHs as potential natural antioxidants for the food industry and contribute to the growing trend of utilizing hempseed by-products.


Assuntos
Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Hidrolisados de Proteína/farmacologia , Antioxidantes/metabolismo , Cannabis/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Endopeptidases/metabolismo , Células HeLa , Humanos , Peróxido de Hidrogênio/farmacologia , Hidrólise , Peptídeo Hidrolases , Peptídeos/metabolismo , Extratos Vegetais/metabolismo , Hidrolisados de Proteína/metabolismo , Espécies Reativas de Oxigênio/metabolismo
18.
Food Chem Toxicol ; 133: 110799, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31493463

RESUMO

Aluminum (Al) is toxic for humans and animals. Here, we have tested the potential for Egg White Hydrolysate (EWH) to protect against cardiovascular changes in rats exposed to both high and low dietary levels of Al. Indeed, EWH has been previously shown to improve cardio metabolic dysfunctions induced by chronic exposure to heavy metals. Male Wistar rats received orally: Group 1) Low aluminum level (AlCl3 at a dose of 8.3 mg/kg b.w. during 60 days) with or without EWH treatment (1 g/kg/day); Group 2) High aluminum level (AlCl3 at a dose of 100 mg/kg b.w. during 42 days) with or without EWH treatment. After Al treatment, rats co-treated with EWH did not show vascular dysfunction or increased blood pressure as was observed in non EWH-cotreated animals. Indeed, co-treatment with EWH prevented the following effects observed in both aorta and mesenteric arteries: the increased vascular responses to phenylephrine (Phe), the decreased ACh-induced relaxation, the reduction on endothelial modulation of vasoconstrictor responses and the nitric oxide bioavailability, as well as the increased reactive oxygen species production from NAD(P)H oxidase. Altogether, our results suggest that EWH could be used as a protective agent against the harmful vascular effects after long term exposure to Al.


Assuntos
Antioxidantes/farmacologia , Proteínas do Ovo/farmacologia , Clara de Ovo/química , Hidrolisados de Proteína/farmacologia , Doenças Vasculares/prevenção & controle , Alumínio , Animais , Antioxidantes/química , Ciclo-Oxigenase 2/metabolismo , Proteínas do Ovo/química , Endotélio Vascular/efeitos dos fármacos , Hidrólise , Masculino , NADPH Oxidases/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos Wistar , Tromboxano-A Sintase/metabolismo , Doenças Vasculares/induzido quimicamente , Vasoconstrição/efeitos dos fármacos
19.
J Dairy Sci ; 102(11): 9598-9604, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31521365

RESUMO

Gastrointestinal conditions in which the transit of contents is altered may benefit from nutritional approaches to influencing health outcomes. Milk proteins modulate the transit of contents along different regions, suggesting that they have varying effects on neuromuscular function to alter gastrointestinal motility. We tested the hypothesis that bovine whey and casein milk protein hydrolysates could have direct modulatory effects on colonic motility patterns in isolated rat large intestine. Casein protein hydrolysate (CPH), whey protein concentrate (WPC), whey protein hydrolysate (WPH), and a milk protein hydrolysate (MPH; a hydrolyzed blend of 60% whey to 40% casein) were compared for their effects on spontaneous contractile waves. These contractions propagate along the length of the isolated intact large intestine (22 cm) between the proximal colon and rectum and were detected by measuring activity at 4 locations. Milk proteins were perfused through the tissue bath, and differences in contraction amplitude and frequency were quantified relative to pretreatment controls. Propagation frequency was decreased by CPH, increased by MPH, and unaffected by intact whey proteins. The reduced motility with CPH and increased motility with MPH indicate a direct action of these milk proteins on colon tissue and provide evidence for differential modulation by hydrolysate type. These findings mirror actions on lower gastrointestinal transit reported in vivo, with the exception of WPH, suggesting that other factors are required.


Assuntos
Caseínas/farmacologia , Colo/efeitos dos fármacos , Trânsito Gastrointestinal , Contração Muscular/efeitos dos fármacos , Proteínas do Soro do Leite/farmacologia , Animais , Bovinos , Intestino Grosso , Masculino , Hidrolisados de Proteína/farmacologia , Ratos , Ratos Sprague-Dawley , Reprodução
20.
Nutrients ; 11(9)2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31509959

RESUMO

Being averse to bitter taste is a common phenomenon for humans and other animals, which requires the pharmaceutical and food industries to source compounds that can block bitterness intensity and increase consumer acceptability. In this work, beef protein alcalase hydrolysates (BPAH) and chymotrypsin hydrolysates (BPCH) were reacted with glucose to initiate Maillard reactions that led to the formation of glycated or advanced glycation end products (AGEs), BPAH-AGEs and BPCH-AGEs, respectively. The degree of glycation was higher for the BPAH-AGEs (47-55%) than the BPCH-AGEs (30-38%). Analysis by an electronic tongue instrument showed that BPAH-AGEs and BPCH-AGEs had bitterness scores that were significantly (p < 0.05) less than quinine. The addition of BPAH-AGEs or BPCH-AGEs to quinine led to significant (p < 0.05) reductions (up to 38%) in bitterness intensity of quinine. The use of 3% hydrolysate to react with glucose yielded glycated peptides with a stronger ability to reduce quinine bitterness than when 1% was used. Calcium release from HEK293T cells stably expressing the T2R4 human bitter taste receptor was significantly (p < 0.05) attenuated by BPAH-AGEs (up to 96%) and BPCH-AGEs (up to 92%) when compared to the BPAH (62%) and BPCH (3%) or quinine (0%). We concluded that BPAH-AGEs and BPCH-AGEs may be used as bitter taste blockers to formulate better tasting foods.


Assuntos
Aromatizantes/farmacologia , Produtos Finais de Glicação Avançada/farmacologia , Proteínas de Carne/farmacologia , Hidrolisados de Proteína/farmacologia , Paladar/efeitos dos fármacos , Sinalização do Cálcio/efeitos dos fármacos , Quimotripsina , Nariz Eletrônico , Aromatizantes/metabolismo , Produtos Finais de Glicação Avançada/metabolismo , Glicosilação , Células HEK293 , Humanos , Reação de Maillard , Proteínas de Carne/metabolismo , Hidrolisados de Proteína/metabolismo , Receptores Acoplados a Proteínas-G/antagonistas & inibidores , Receptores Acoplados a Proteínas-G/metabolismo , Subtilisinas/metabolismo
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