Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 977
Filtrar
1.
Food Chem ; 315: 126202, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32028197

RESUMO

The identification and quantification of soluble- and insoluble-bound phenolics in lentil hulls were studied using HPLC-DAD-ESI-MSn and their antioxidant potential determined using DPPH radical scavenging ability (DRSA), reducing power (RP), and hydroxyl radical scavenging ability (HRSA) assays to test their electron and hydrogen donating abilities. A number of soluble phenolics such as phenolic acids, flavonoids, and proanthocyanidins were found, which lead to the remarkable antioxidant potential as reflected in DRSA, RP, and HRSA. Meanwhile, insoluble-bound phenolics displayed a relatively lower number of peaks and contents than their corresponding soluble phenolics, leading to a lower antioxidant potential than that of soluble phenolics. Moreover, dihydrokaempferol dimer and carboxylated kaempferol diglucoside were identified for the first time in the insoluble-bound form in lentils. This study offers important data for the identification of phenolic compounds derived from lentils and their antioxidant potential.


Assuntos
Antioxidantes/análise , Cromatografia Líquida de Alta Pressão/métodos , Lens (Planta)/química , Fenóis/análise , Espectrometria de Massas em Tandem/métodos , Antioxidantes/química , Flavonoides/análise , Depuradores de Radicais Livres/análise , Hidroxibenzoatos/análise , Radical Hidroxila/química , Proantocianidinas/análise , Sementes/química , Solubilidade , Espectrometria de Massas por Ionização por Electrospray
2.
Food Chem ; 315: 126270, 2020 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-32028199

RESUMO

An electrochemistry coupled to online quadrupole time-of-flight tandem mass spectrometry (EC/Q-TOF/MS) was applied to investigate the oxidative transformation and metabolic pathway of five phenolic acids in Danshen sample. Simulation of the phase I oxidative metabolism was carried out in an electrochemical reactor equipped with a glassy carbon working electrode. The phase II reactivity of the generated oxidative products towards biomolecules (such as glutathione) was investigated by ways of covalent adduct formation experiments. The results obtained by EC/MS were compared with well-known in vitro studies by conducting rat liver microsome incubations. Structures of the electrochemically produced metabolites were identified by accurate mass measurement and previously results in vivo metabolites. It was indicated that the electrochemical oxidation was in good accordance with similar products found in vivo experiments. In conclusion, this work confirmed that EC/Q-TOF/MS was a promising analytical tool in the prediction of metabolic transformations of functional foods.


Assuntos
Técnicas Eletroquímicas/métodos , Hidroxibenzoatos/farmacocinética , Salvia miltiorrhiza/química , Espectrometria de Massas em Tandem/métodos , Animais , Eletrodos , Glutationa/metabolismo , Hidroxibenzoatos/análise , Hidroxibenzoatos/metabolismo , Masculino , Microssomos Hepáticos/metabolismo , Oxirredução , Ratos Sprague-Dawley
3.
J Photochem Photobiol B ; 203: 111768, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31931383

RESUMO

In vitro callus cultures of Verbena officinalis L. were maintained on solid Murashige and Skoog medium, enriched with 1 mg dm-3 BA and 1 mg dm-3 IBA under LED lights (red, blue, red/blue 70%/30%), in darkness and under control fluorescent lamps. The measurements of 2 phenylpropanoid glycosides (verbascoside and isoverbascoside) and 23 phenolic acids were performed in methanolic extracts from the biomass collected after 2-, 3- and 4-week growth cycles using the HPLC-DAD method. The presence of verbascoside, isoverbascoside and additionaly 7 phenolic acids (protocatechuic, chlorogenic, vanillic, caffeic, ferulic, o-coumaric and m-coumaric acids) was confirmed in all extracts. Blue and red/blue lights stimulated the accumulation of verbascoside (max. of 6716 and 6023 mg 100 g-1 DW after a 4-week growth cycle) and isoverbascoside (max. 333 and 379 mg 100 g-1 DW also after 4 weeks). The maximum amounts of verbascoside and isoverbascoside were respectively 1.8- and 7.0-fold higher than under the control conditions. Phenolic acids were accumulated in different amounts, and the maximum total amounts ranged from 36 to 65 mg 100 g-1 DW. LED lights also stimulated their accumulation in comparison with darkness and control. The main phenolic acids included: m-coumaric acid (max. 39 mg 100 g-1 DW), ferulic acid (max. 12 mg 100 g-1 DW), and protocatechuic acid (max. 13 mg 100 g-1 DW). Additionally, the quantities of photosynthetic pigments (chlorophyll a, b and carotenoids) were estimated in acetonic extracts using spectrophotometry. Red/blue light stimulated the biosynthesis of pigments (max. total content 287 µg g-1 FW after 4-week growth cycles). This is the first study describing the effect of LED lights on the production of phenylpropanoid glycosides and phenolic acids in V. officinalis callus cultures. Very high amounts of verbascoside and isoverbascoside are interesting from a practical point of view.


Assuntos
Glucosídeos/metabolismo , Hidroxibenzoatos/metabolismo , Luz , Fenóis/metabolismo , Verbena/metabolismo , Biomassa , Carotenoides/análise , Clorofila/análise , Clorofila A/análise , Cromatografia Líquida de Alta Pressão , Glucosídeos/análise , Hidroxibenzoatos/análise , Fenóis/análise , Fotossíntese/efeitos da radiação , Análise de Componente Principal , Análise de Regressão , Espectrofotometria
4.
Food Chem ; 312: 126046, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31911354

RESUMO

The impact of crop level and leaf removal on Istrian Malvasia (Vitis vinifera L.) white wine aroma and phenolic acids composition was studied over two vine-growing seasons. Two crop levels were combined with leaf removal or untreated control in two-factorial design. Crop level showed limited impact on aroma compounds in wine and the concentration of some esters was increased by higher crop level. In contrast, leaf removal increased the concentration of several aroma compounds and especially monoterpenes and esters. The concentration of hydroxycinnamic acids in wine was enhanced only by leaf removal, while no consistent impact of the investigated factors on hydroxybenzoic acids was observed. The obtained results suggest that in cases where environmental conditions are not limiting, increasing the crop level under adequate microclimate in fruit zone has no detrimental effects on white wine aroma and hydroxycinnamic acids composition, potentially leading to economically more sustainable grape production.


Assuntos
Hidroxibenzoatos/análise , Odorantes/análise , Vitis/química , Vinho/análise , Éteres/análise , Frutas/química , Monoterpenos/análise
5.
Sci Total Environ ; 704: 135462, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-31810671

RESUMO

The responses in growth and defense after tissue damage are highly variable in plants depending on species, damaged-tissue type and the intensity of damage. The prevailing abiotic conditions can also influence these responses. In this study, our aim was to examine how the removal of lateral vegetative buds affects the growth and accumulation of phenolics in saplings of the dioecious Populus tremula grown under simulated climate change. For three growing seasons, the saplings were grown under ambient conditions (control), elevated temperature (+2°C) and elevated UV radiation (30%) (UVB and UVA as its control), or a combination of these. In the fourth growing season, all saplings were grown under ambient conditions. The bud removal was performed twice - in summer and autumn - in the third year. The responses of growth and the accumulation of phenolics to the bud removal were measured at the end of the fourth growing season. Removal of 5% of the lateral buds resulted in higher leaf, stem and total plant biomass in both sexes of P. tremula saplings, compared to intact plants. The effects were greater in the temperature-treated plants, especially in the temperature-treated females. The concentrations of flavonoids and condensed tannins were higher in the bud-removed individuals. The concentration of condensed tannins was also higher in the males than in the females, opposite to the concentration of phenolic acids. There was no significant interaction between bud removal and UVB treatment on either growth or phenolics. Our results suggest that plants can allocate resources to both growth and defense simultaneously in response to tissue loss, and that global warming can modify the responses to some extent.


Assuntos
Mudança Climática , Hidroxibenzoatos/análise , Populus , Aquecimento Global , Fenóis/análise , Folhas de Planta/química , Estações do Ano , Temperatura , Raios Ultravioleta
6.
Chem Biodivers ; 17(1): e1900394, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31618522

RESUMO

We aimed in the present study to investigate the chemical composition, the antioxidant capacities as well as the in vitro fermentation properties of Salvia officinalis leaves aqueous extract (SOLAE) grown in four regions of northwestern Tunisia. Our data firstly indicated a spatial variation (P<0.05) in condensed tannins, total lipids, polyphenols and flavonoids contents. The HPLC-PDA-ESI-MS/MS-LC/HR-ESI-MS technique allowed to the identification of 13 phenolic compounds and showed that protocatechuic acid is the major constituent of the plant leaves grown in Tabarka, Ain Draham and Testour. The SOLAE of the plant grown in Tabarka presents the most potent scavenging activity against DPPH radical and had the highest percentage of inhibition. More importantly, we found in the present study that the digestibility of dry matter and in vitro fermentation showed a significant variation between the regions and the animal species. Also, we showed a very positive correlation between antioxidant properties and phenolic compounds contents. In conclusion, we suggest that SOLAE had potential beneficial effects owing in part to its antioxidant and ROS scavenging activities. Therefore, S. officinalis can be proposed as an additive food for animals' nutrition and health.


Assuntos
Antioxidantes/farmacologia , Compostos de Bifenilo/antagonistas & inibidores , Fermentação , Compostos Fitoquímicos/farmacologia , Picratos/antagonistas & inibidores , Salvia officinalis/metabolismo , Animais , Antioxidantes/análise , Antioxidantes/metabolismo , Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão , Avaliação Pré-Clínica de Medicamentos , Cabras , Hidroxibenzoatos/análise , Hidroxibenzoatos/metabolismo , Hidroxibenzoatos/farmacologia , Espectrometria de Massas , Fenol/análise , Fenol/metabolismo , Fenol/farmacologia , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/metabolismo , Picratos/metabolismo , Folhas de Planta/química , Folhas de Planta/metabolismo , Salvia officinalis/química , Salvia officinalis/crescimento & desenvolvimento , Ovinos , Tunísia
7.
Biomed Chromatogr ; 34(1): e4714, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31633806

RESUMO

Eucommia ulmoides Oliv. (E. ulmoides) is a valuable and nourishing medicinal herb in China that has been used in the treatment of hypertension. Given the fact that most traditional Chinese medicine is mainly used to treat disease, investigating the pharmacokinetics of traditional Chinese medicines in the pathological state is more useful than that in the normal state. However, the differences in the absorption kinetics of active ingredients of E. ulmoides extract between pathological and physiological conditions have not been reported. Therefore, in this study, the rat intestinal in situ circulatory perfusion model was used to investigate the differences in absorption kinetics of seven active ingredients of E. ulmoides extract in normal and spontaneously hypertensive rats, namely, genipinic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, (+)-pinoresinol di-O-ß-D-glucopyranoside and (+)-pinoresinol 4'-O-ß-D-glucopyranoside. Our results indicate that the pathological state of spontaneous hypertension may change the absorption of active components of E. ulmoides extracts, and these findings may provide a reference for improving the rational use of E. ulmoides in the clinic.


Assuntos
Eucommiaceae , Absorção Intestinal , Extratos Vegetais , Animais , Anti-Hipertensivos/análise , Anti-Hipertensivos/farmacocinética , Líquidos Corporais/química , Ácido Clorogênico/análogos & derivados , Ácido Clorogênico/análise , Ácido Clorogênico/farmacocinética , Furanos/análise , Furanos/farmacocinética , Hidroxibenzoatos/análise , Hidroxibenzoatos/farmacocinética , Lignanas/análise , Lignanas/farmacocinética , Extratos Vegetais/análise , Extratos Vegetais/farmacocinética , Ratos , Ratos Endogâmicos SHR , Ratos Wistar
8.
Chem Biodivers ; 17(1): e1900465, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31701649

RESUMO

We performed comparative profiling of four specialized metabolites in the lichen Evernia prunastri, collected at three different geographic locations, California and Maine, USA, and Yoshkar Ola, Mari El, Russia. Among the compounds produced at high concentrations that were identified in all three specimens, evernic acid, usnic acid, lecanoric acid and chloroatranorin, evernic acid was the most abundant. Two depsidones, salazinic acid and physodic acid, were detected in the Yoshkar-Ola collection only. The crystalline structure of evernic acid (2-hydroxy-4-[(2-hydroxy-4-methoxy-6-methylbenzoyl)oxy]-6-methylbenzoate) (hmb) revealed two crystallographically and conformationally distinct hmb anions, along with two monovalent sodium atoms. One hmb moiety contained an exotetradentate binding mode to sodium, whereas the other exhibited an exohexadentate binding mode to sodium. Embedded edge-sharing {Na2 O8 }n sodium-oxygen chains connected the hmb anions into the full three-dimensional crystal structure of the title compound. The crystal used for single-crystal X-ray diffraction exhibited non-merohedral twinning. The data suggest the importance of the acetyl-polymalonyl pathway products to processes of maintaining integrity of the lichen holobiont community.


Assuntos
Benzofuranos/análise , Hidroxibenzoatos/análise , Líquens/química , Salicilatos/análise , Benzofuranos/metabolismo , Hidroxibenzoatos/metabolismo , Líquens/metabolismo , Modelos Moleculares , Salicilatos/metabolismo
9.
Food Chem ; 306: 125644, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31610329

RESUMO

Far infrared radiation (FIR) and hot air drying (HA) were applied to unpolished and polished pigmented rice varieties. Ferulic acid is a major phenolic acid in pigmented rice, being three-fold greater in bound form than in soluble form. Overall, FIR increased total phenolic and flavonoid contents, anthocyanin, tocopherols as well as antioxidant capacity, whereas the opposite results were observed for HA in all samples. Gallic, and ferulic acids were significantly (p < 0.05) increased in FIR dried samples. Quercetin content was significantly increased in unpolished rice dried by FIR and HA. Cyanidin-3-glucoside was greatly increased by FIR. Amino acids were decreased less by HA than did FIR. Our findings suggest that HA and FIR may have a significant effect on the internal structure of the grain, which may increase the yield of extraction of some components, including the bioactives.


Assuntos
Aminoácidos/química , Oryza/química , Ar , Aminoácidos/metabolismo , Antocianinas/análise , Antioxidantes/análise , Ácidos Cumáricos/análise , Grão Comestível/química , Hidroxibenzoatos/análise , Raios Infravermelhos
10.
Food Chem ; 311: 125940, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31864187

RESUMO

Wheat (Triticum spp. L.) is an important source of nutrients and bioactive compounds with recognized beneficial effects. Wheat undergoes several processes with the final aim of separating the endosperm from the outer layers, usually discarded. In this study, free and bound phenolic acids (PAs) profile, betaine and choline contents were quantified in six different wheat species (durum and bread wheat, turanicum wheat, einkorn, emmer and spelt), the corresponding milling by-products (bran, middlings, aleurone and I, II and III steps of debranning) and flour/semolina, using UHPLC-MS/MS methods. The bound form of phenolics was the component present in higher concentration (80% of the total, in average) and ferulic acid was the most abundant compounds, representing between 67 and 73 % of total PAs. Among the species, bread wheat grain totalized the highest content of total PAs (1209.31 ± 7.3 µg g-1 d.w.). Betaine and choline are abundantly present in wheat species. In general, the highest content of bioactive compounds was found in bran (3 times higher than whole grains), emphasizing the good nutritional profile of these by-products. The milling process leads to a severe reduction of phenolic acids and methyl-donors in the end-products.


Assuntos
Betaína/análise , Colina/análise , Manipulação de Alimentos/métodos , Hidroxibenzoatos/análise , Triticum/química , Pão/análise , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos/análise , Farinha/análise , Fenóis/análise , Sementes/química , Espectrometria de Massas em Tandem , Resíduos/análise , Grãos Integrais/química
11.
Food Chem ; 311: 125969, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31864186

RESUMO

The polyphenolic content in date seeds, a promising functional ingredient for food, was characterised in three forms viz., date seed powder (DSP), date seed pita bread (DSB) and date seed extract (DSE). Bioaccessibility of the polyphenols from the samples was assessed by in-vitro digestion coupled with transport using Caco-2 cells. HPLC-ESI-UV/MS/MS-(IT) analysis recorded the presence of phenolic acids, flavanols, flavonols and flavones. Flavan-3-ols was the most significant group with the highest concentration in DSP, 47.91 ± 0.13 g/kg, after depolymerisation. Phenolic acids such as protocatechuic acid, vanillic acid and caffeoylshikimic acid were recovered from DSP and DSE after in-vitro digestion. In comparison, the recovery was significantly lower in the bread sample. Similarly, transport of protocatechuic acid, p-hydroxybenzoic acid, caffeoylshikimic acid, p-coumaric acid, syringic acid hexoside and diosmin through Caco-2 monolayer was observed in DSP and DSE, while protocatechuic acid and p-hydroxybenzoic acid were the only polyphenols transported from digested DSB.


Assuntos
Cromatografia Líquida de Alta Pressão , Phoeniceae/química , Polifenóis/análise , Espectrometria de Massas em Tandem , Células CACO-2 , Flavonas/análise , Flavonóis/análise , Humanos , Hidroxibenzoatos/análise , Phoeniceae/metabolismo , Polifenóis/metabolismo , Sementes/química , Sementes/metabolismo
12.
J Oleo Sci ; 68(11): 1063-1071, 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31611511

RESUMO

In this study, some physico-chemical properties, amino acids, fatty acids, sugars and mineral contents of sorghum, millet and lupin seeds. Sorghum (red, white and yellow) and millet seeds were purchased from market in Saudi Arabia (Riyadh). Lupin seeds were provided from in Turkey (Konya). Protein contents of seed samples ranged from 8.6% (yellow sorghum) to 37.7% (lutop) (p < 0.05). The extractable phenolics contents for gallic acid equivalent (GAE) of grains ranged between 1.43 mgGAE/g (white sorghum) to 8.23 mgGAE/g (red sorghum), and hydrolysable phenolics contents for GAE of grains varied between 1.48 mgGAE/g (white sorghum) to 26.10 mgGAE/g (red sorghum (p < 0.05). Total phenol contents of seeds were found between 2769 mg GAE/g (bablon) to 6087 mgGAE/g (yellow sorghum) (p < 0.05). Amino acid contents of millet changed between 0.02% (ornithine) and 2.07% (glutamic acid), while amino acid contents of yellow sorghum range from 0.02% (hydroxyproline) to 1.71% (glutamic acid), amino acid values of white sorghum changed between 0.02% (hydroxyproline) and 2.21% (glutamic acid), amino acid values of lutop seed changed between 0.02% (ornithine) and 6.77% (glutamic acid) (p < 0.05).While the oleic acid contents change between 25.27% (white sorghum) and 53.50% (Bablone), linoleic acid contents ranged from 14.60% (Bablone) to 42.67% (Millet) (p < 0.05). However, the amount of potassium in the seeds varied between 1831.34 mg/kg (while sorghum) and 11895.8 mg/kg (Lutop). Generally, protein, oleic acid, amino acid and mineral contents of lupin varieties were higher as compared to those of millet phenol, anthocyanin and sorghum seeds.


Assuntos
Aminoácidos/análise , Carboidratos/análise , Ácidos Graxos/análise , Lupinus/química , Milhetes/química , Minerais/análise , Sementes/química , Sorghum/química , Fenômenos Químicos , Ácido Gálico/análise , Hidroxibenzoatos/análise , Proteínas de Plantas/análise , Arábia Saudita , Turquia
13.
J Biotechnol ; 305: 61-70, 2019 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-31494211

RESUMO

In vitro cultures and raw materials (fruits and leaves) of the valuable medicinal plant species - Schisandra chinensis cultivar Sadova No. 1 (SchS) - were evaluated for the production of two groups of phenolic compounds, phenolic acids and flavonoids, and their antioxidant potential. A series of experiments was conducted, aimed at optimizing culture conditions for maximum growth and phenolic production in SchS microshoots. Different concentrations of plant growth regulators (6-benzyladenine - BA and 1-naphthaleneacetic acid - NAA, from 0 to 3 mg/l) in Murashige-Skoog (MS) medium were tested in several cultivation systems (agar, agitated, bioreactor) over various growth periods (10, 20, 30, 40, 50 and 60 days). Furthermore, an elicitation experiment was conducted in which the bioreactor-grown microshoots were exposed to yeast extract. HPLC-DAD analyses confirmed the presence of eight phenolic acids - chlorogenic, cryptochlorogenic, gallic, neochlorogenic, protocatechuic, salicylic, syringic and vanillic, and two flavonoids: kaempferol and quercitrin, in the in vitro biomasses. The highest total phenolic acid (357.93 mg/100 g DW) and flavonoid (105.07 mg/100 g DW) contents were obtained in agar culture extracts cultivated for 30 days on MS medium containing 2 mg/l BA and 0.5 mg/l NAA and for 50 days on MS medium containing 0.1 mg/l BA and 2 mg/l NAA, respectively. These amounts were 1.59- and 5.95-fold lower than in parent plant leaf extracts (569.66 mg/100 g DW), and 4.30- and 1.25-fold higher than in fruit extracts (83.17 mg/100 g DW), respectively. Microshoots grown in a Plantform bioreactor also proved to be a good source of phenolic compounds, however, the elicitor treatment had no noticeable effect on their accumulation. Antioxidant capacity assessed by the Folin-Ciocalteu, FRAP, DPPH and CUPRAC assays revealed significantly higher potential in extracts from in vitro biomass and leaves of the parent plant, as compared to the parent plant fruit extracts.


Assuntos
Flavonoides/análise , Hidroxibenzoatos/análise , Schisandra/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos/métodos , Ágar , Reatores Biológicos , Meios de Cultura/química , Meios de Cultura/farmacologia , Reguladores de Crescimento de Planta/farmacologia , Brotos de Planta/crescimento & desenvolvimento , Plantas Medicinais/química , Plantas Medicinais/crescimento & desenvolvimento , Schisandra/química , Leveduras/química
14.
Sci Total Environ ; 694: 133387, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31386951

RESUMO

Baobab (Adansonia digitata) fruit is a part of the baobab tree, a revered multi-purpose tree native to Africa with a myriad of potentials in providing shelter, food, medicine, clothing and as a valuable source of natural antioxidants. A massive quantity of baobab fruit shells (BFS) is generated as a waste from the baobab fruit processing representing significant economic and environmental challenges at the same time an opportunity for its valorization and commercial utilization. The present study explored the potentials of BFS as a source of phenolic compounds. For this purpose, the phenolic constituents of BFS were identified by LC-MS/QTOF analysis. Also, the Total Phenolic Chromatographic Index (TPCI), TPC, TFC, and antioxidant capacity were compared with the baobab seeds and pulp. The antidiabetic potential through α-amylase and α-glucosidase inhibitory activities was also compared with that of acarbose. The LC-MS/QTOF analysis led to the identification of 45 compounds, including quercetin, kaempferol, proanthocyanidins, phenolic acids and their derivatives, several of which had never been reported in baobab fruit. Moreover, the BFS showed higher TPC, TFC, and antioxidant capacity than the baobab seeds and pulp and inhibited α-amylase and α-glucosidase enzymes activities with much higher potency than acarbose. This research demonstrated the promising potentials of BFS as a good source of phenolic compounds that can further be utilized for food and pharmaceutical applications.


Assuntos
Adansonia/química , Frutas/química , Hidroxibenzoatos/análise , África , Fenóis/análise , Extratos Vegetais/química , Folhas de Planta/química
15.
Molecules ; 24(15)2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31387251

RESUMO

The Artemisia argyi leaf (AL) has been used as a traditional medicine and food supplement in China and other Asian countries for hundreds of years. Phytochemical studies disclosed that AL contains various bioactive constituents. Among bioactive constituents, phenolic acids have been recognized as the main active compounds in AL. To the best of our knowledge, no research has been focused on extraction method for the bioactive phenolic acids from AL. Nowadays, deep eutectic solvents (DESs) are emerging as a new type of green and sustainable solvent for efficient extraction of bioactive compounds from natural products. In the present study, an environmentally friendly extraction method based on DESs was established to extract bioactive phenolic acids from ALs. Diverse tailor-made solvents, including binary and ternary DESs, were explored for simultaneous extraction of four phenolic acids (3-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid) from AL. The results indicated that the ternary DES composed of a 2:1:2 molar ratio of choline chloride, malic acid, and urea showed enhanced extraction yields for phenolic acids compared with conventional organic solvents and other DESs. Subsequently, the extraction parameters for the four phenolic acids by selected tailor-made DESs, including liquid-solid ratios, water content (%) in the DESs, and extraction time, were optimized using response surface methodology and the optimal extraction conditions were: extraction time, 23.5 min; liquid-solid ratio, 57.5 mL/g (mL of DES/g dry weight of plant material); water content, 54%. The research indicated that DESs were efficient and sustainable green extraction solvents for extraction of bioactive phenolic acids from natural products. Compared to the conventional organic solvents, the DESs have a great potential as possible alternatives to those organic solvents in health-related areas such as food and pharmaceuticals.


Assuntos
Artemisia/química , Hidroxibenzoatos/química , Extratos Vegetais/química , Folhas de Planta/química , Solventes/química , Análise de Variância , Cromatografia , Hidroxibenzoatos/análise , Estrutura Molecular , Extratos Vegetais/análise , Análise Espectral
16.
Food Chem ; 301: 125228, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31377613

RESUMO

Hexaploid tritordeum is the amphidiploid cereal derived from the cross between wild barley and durum wheat. The present study compares two cultivars of tritordeum with other cereals grown in the same experimental area to weigh up its potential use as ingredient for health-valued foods. Tritordeum shows 2.5-fold higher concentration of lutein than common wheat and barley, and 1.2-fold higher than durum wheat, while the concentration of ß-glucans is 5 folds lower than the one observed for barley. Based on the distribution of bioactive compounds in pearled fractions, the use of whole-grain flours seems the best way to exploit the antioxidant potential of tritordeum. Nevertheless, the internal layers of the kernel of this cereal are characterized on average by high concentrations of antioxidants (32.0 mg/kg and 518 mg/kg soluble and cell wall-bound phenolic acids, respectively), making tritordeum interesting also for the production of refined flours rich in bioactive compounds.


Assuntos
Triticum/química , Antioxidantes/análise , Farinha/análise , Hidroxibenzoatos/análise , Luteína/análise , Solubilidade , Grãos Integrais/química , beta-Glucanas/análise
17.
Food Chem ; 299: 125129, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31299518

RESUMO

Three varieties of sweetpotato flour (Orange Sunset (OS), Purple Dawn (PD), and Red) were treated by high hydrostatic pressure (HHP). Thermal analysis showed that complete starch gelatinization occurred in PD and Red subjected to 600 MPa. Starch of OS was partially gelatinized at 600 MPa. The pressure of 600 MPa caused significant decreases in peak viscosity, breakdown and setback, and an increase in pasting temperature. Compared with native samples, HHP-treated samples showed higher in vitro starch digestibility in uncooked conditions but lower digestibility in cooked conditions. HHP significantly improved the extractability of bioactive compounds from sweetpotato flour. The changes in ß-carotene content, total phenolic content, in vitro antioxidant activities, individual phenolic acids, and anthocyanins profiles were investigated. This study suggests the potential of HHP as a non-thermal processing tool to modify the functional properties of sweetpotato flour.


Assuntos
Farinha , Ipomoea batatas/química , Farinha/análise , Indústria de Processamento de Alimentos/métodos , Gelatina/química , Pressão Hidrostática , Hidroxibenzoatos/análise , Especificidade da Espécie , Amido/química , Temperatura , Viscosidade , beta Caroteno/análise
18.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2059-2064, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355561

RESUMO

To construct a quality management model for the whole industry chain of compound Danshen Tablets,and quality control system for all key links in the production of compound Danshen Tablets. In this paper,with salvianolic acid B as internal reference substance,three batches of mix standards were prepared,and three sets of relative correlation factors between salvianolic acid B and other phenolic acids were calculated in parallel. Finally,the correlation factors are obtained on average. The quality transfer process was studied by optimizing the concentration of Salvia miltiorrhiza extract. The results showed that RSD among three sets of relative correlation factors ranged between 1. 7%-4. 1%,with no significant difference between the quantitative result of two methods. In addition,the quality transfer study showed that with the rise of the concentration temperature,the content of phenolic acid components changed,which had a significant effect on the salvianolic acid B at more than 80 ℃. It was suggested to rationally control the concentration temperature during the industrial production. The results of this study provide a methodology for the establishment of the quality control system for the whole industry chain of compound Danshen Tablets,and quality control methods for the improvement of the quality of medicinal materials and finished medicine products.


Assuntos
Medicamentos de Ervas Chinesas/química , Hidroxibenzoatos/análise , Salvia miltiorrhiza/química , Cromatografia Líquida de Alta Pressão , Controle de Qualidade , Comprimidos
19.
Zhongguo Zhong Yao Za Zhi ; 44(12): 2499-2510, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31359717

RESUMO

Ten batches of Angelica sinensis from three producing areas( Tuoxiang,Minxian and Weiyuan of Gansu province) were selected as the research objects,and processed into raw A. sinensis,A. sinensis with alcohol,and A. sinensis with soil respectively through the standard processing methods. Ultra-high performance liquid chromatography( UPLC) was used to establish fingerprint for three processed products of A. sinensis,and determine the contents of 9 phenolic acids and phthalide compounds. The similarity was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine,which showed that the chromatographic peaks of the same processed samples of A. sinensis were basically similar,with all similarities greater than 0. 950. The difference between different processed products and their control spectra was not obvious,with all similarities also higher than 0. 950.On the basis of using principal component analysis( PCA) and OPLS-DA to seek the difference components between groups,the improved distance coefficient method can be used to effectively distinguish the three processed products of A. sinensis by fingerprint similarity. At the same time,the determination method of nine phenolic acids and phthalide in A. sinensis was established by UPLC,and the comparison between different processed products was carried out. The results showed that the content of various components was changed as compared with the raw A. sinensis. The contents of coniferyl ferulate and ligustilide in the A. sinensis with alcohol were increased significantly,and the content of coniferyl ferulate was obviously increased in A. sinensis with soil. The method established in this paper can effectively distinguish different processed products of A. sinensis and determine the content of the main components in them.


Assuntos
Angelica sinensis/química , Medicamentos de Ervas Chinesas/análise , 4-Butirolactona/análogos & derivados , 4-Butirolactona/análise , Benzofuranos/análise , Cromatografia Líquida de Alta Pressão , Ácidos Cumáricos/análise , Hidroxibenzoatos/análise , Medicina Tradicional Chinesa , Análise de Componente Principal
20.
J Dairy Sci ; 102(8): 6790-6801, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31178182

RESUMO

This study proposed the General Health Index of Cheese (GHIC) as an indicator for the presence of health-promoting compounds in cheese and compared the antioxidant capacity and phenolic and fatty acid contents of cheeses from goats consuming 7 forage species. Ninety-one homogeneous Red Syrian goats were randomly assigned to 1 of 7 feeding treatments (Festuca arundinacea, Hordeum vulgare, Triticosecale, Pisum sativum, Trifolium alexandrinum, Vicia sativa, and Vicia faba minor). The housed goat groups received the scheduled forage ad libitum. Forage species affected the antioxidant capacity, the phenolic and fatty acid contents, the Health Promoting Index, and the GHIC. Trifolium alexandrinum, Triticosecale, and Hordeum vulgare showed a clear advantage in terms of beneficial fatty acids content in goat cheese. Cheese from the Triticosecale group also showed a high antioxidant capacity value even if its polyphenol content was intermediate compared with others. Trifolium alexandrinum and Triticosecale had the highest value of the new index GHIC. This comparison suggests that there are important differences in fatty acid profile and polyphenol content among cheeses from goats fed grasses and legumes commonly used in the Mediterranean area. In this first approach, GHIC index, which combines the positive components found in cheese, seems to be a useful tool to provide an indication concerning the general health value of the product.


Assuntos
Ração Animal , Queijo/análise , Cabras/metabolismo , Animais , Antioxidantes/análise , Ácidos Graxos/análise , Festuca , Hordeum , Hidroxibenzoatos/análise , Leite/química , Ervilhas , Polifenóis , Trifolium , Triticale , Vicia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA