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1.
Allergol. immunopatol ; 47(4): 350-356, jul.-ago. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-186506

RESUMO

Introduction and objectives: Biological aerosols play a vital role in the interactions between the atmosphere, biosphere, climate and public health and fungal spores are a component with allergic importance. We constructed a database in Castile & Leon (Spain) and carry out molecular-level component-resolved diagnosis to complete the air quality study carried out since 2006 by our aerobiological network (RACYL) to aid clinical diagnosis and treatment. Methods: We reviewed a database of 19,774 patients (adults and children) with allergic respiratory disease treated in our unit during the last 12 years. We also made a component-resolved diagnosis of the molecules involved in the pathology in a randomly selected population of 150 patients. Results: The dimeric glycoprotein Alt a1 from Alternaria is the most prevalent and most useful allergen in the diagnosis of patients with allergy to fungi in our area (94.4%), followed by enolase Alt a 6 (Alternaria), ribonuclease Asp f 1 of Aspergillus and mannitol dehydrogenase from Cla h 8 (Cladosporium). Conclusions: Our results have helped determine which spore molecules are most-closely associated with allergies. Molecular analysis will be useful to determine more accurate and useful immunotherapy in these patients


No disponible


Assuntos
Humanos , Masculino , Feminino , Criança , Adulto , Alternariose/epidemiologia , Aspergilose/epidemiologia , Hipersensibilidade Respiratória/epidemiologia , Esporos Fúngicos/imunologia , Tinha/epidemiologia , Alérgenos/imunologia , Alternaria/imunologia , Antígenos de Fungos/imunologia , Aspergilose/imunologia , Cladosporium/imunologia , Bases de Dados Factuais , Espanha/epidemiologia
2.
Rev Mal Respir ; 36(7): 889-901, 2019 Sep.
Artigo em Francês | MEDLINE | ID: mdl-31303366

RESUMO

Exposure to mould is a potential risk factor for asthma in both adults and children. In adult, the relation between exposure, sensitization and symptoms has been demonstrated in severe asthmatics sensitized to Alternaria. For children, exposure to mould in childhood is a risk factor for asthma in both atopic and non-atopic individuals. Exposure or sensitization to moulds are a risk factor for severe asthma and/or exacerbations in children. There appears to be a causal relationship between exposure and asthma. This link seems less significant in adults. However, in adults mould sensitive asthma seems to determine a phenotype of severe asthma associated with more marked obstructive lung disease. Moulds can stimulate either innate or the acquired immunity. They are responsible for a marked Th2 inflammation leading to more severe asthma. Besides the immunological mechanisms, toxic mechanisms can also intervene. It is therefore not correct to reduce the effect of moulds, particularly in respiratory symptoms, to only allergic mechanisms.


Assuntos
Poluição do Ar em Ambientes Fechados/efeitos adversos , Características da Família , Fungos , Pneumopatias Fúngicas/etiologia , Hipersensibilidade Respiratória , Adulto , Poluição do Ar em Ambientes Fechados/estatística & dados numéricos , Asma/epidemiologia , Asma/etiologia , Criança , Exposição Ambiental/efeitos adversos , Exposição Ambiental/estatística & dados numéricos , Humanos , Pneumopatias Fúngicas/epidemiologia , Material Particulado/efeitos adversos , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/etiologia , Fatores de Risco
3.
Aust Vet J ; 97(9): 343-350, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31286483

RESUMO

OBJECTIVE: To investigate the relationship between bronchoalveolar lavage fluid cytology, particularly mast cells, and airway hyper-reactivity in athletic horses presented for poor performance that included a respiratory tract evaluation in two disparate locations in Australia. DESIGN: Multi-centre, retrospective and prospective cross-sectional study METHODS: Eighty four adult horses underwent both pulmonary function testing and histamine bronchoprovocation with a commercial flowmetric plethysmography system. A bronchoalveolar lavage was performed four to twelve hours later. Bronchoalveolar lavage fluid cytology was categorised using two differing classification systems to define mild equine asthma. Statistical analysis was used to assess associations between bronchoalveolar lavage fluid relative inflammatory cell percentages, and airway hyper-reactivity and their associated categorisations. RESULTS: Sixty four percent (54/84) of horses displayed airway hyper-reactivity, as defined by PC35 < 6 mg/ml of histamine. A relative mastocytosis was the most common bronchoalveolar lavage fluid cytological abnormality. Horses with a sole mast cell response of ≥ 5% within their bronchoalveolar lavage fluid displayed airway hyper-reactivity at a lower dose of nebulized histamine than horses with normal bronchoalveolar lavage fluid cytology. Horses with mixed cell responses (relative mast cell percentage > 2% and/or relative neutrophil percentage > 5% and/or eosinophil relative cell percentage ≥ 1%) displayed airway hyper-reactivity at a lower dose of nebulized histamine than horses with normal bronchoalveolar lavage fluid cytology. CONCLUSION: In the Australian context, recently revised increased bronchoalveolar lavage fluid cytology relative cell percentage cut offs appear appropriate for sole mast cell responses. The historical lower cut offs appear to be appropriate for mixed inflammatory cell responses.


Assuntos
Asma/veterinária , Líquido da Lavagem Broncoalveolar/citologia , Doenças dos Cavalos/fisiopatologia , Animais , Asma/epidemiologia , Asma/fisiopatologia , Desempenho Atlético/fisiologia , Austrália/epidemiologia , Lavagem Broncoalveolar , Estudos Transversais , Doenças dos Cavalos/epidemiologia , Cavalos , Testes de Função Respiratória/veterinária , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/fisiopatologia , Hipersensibilidade Respiratória/veterinária
6.
Environ Int ; 128: 63-69, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31029980

RESUMO

BACKGROUND: It has been suggested that adults who use cleaning agents in their homes have a higher risk of asthma and allergic symptoms. The associations of asthma and allergic symptoms with household use of cleaning agents in adolescents have not been investigated yet. OBJECTIVES: To examine the associations of household cleaning agents use with the prevalence of asthma, rhinitis and eczema in adolescents. METHODS: In this cross-sectional analysis, we included participants of the PIAMA birth cohort study with data on household use of 10 types of cleaning agents and information on asthma, rhinitis and/or eczema from parent-completed questionnaires at age 14 (N = 2333). For the cleaning agents, we developed a composite score ranging from 0 (no exposure) to 30 points (household use on 4-7 days per week for all 10 types of cleaning agents). Logistic regression was used to analyse associations between household cleaning agents use (composite score and specific cleaning agents) and outcomes, adjusting for potential confounders. RESULTS: Seven, 13 and 11% of the participants had asthma, rhinitis and eczema, respectively, at age 14. The composite score for household use of cleaning agents was not associated with asthma, rhinitis and eczema. For instance, adjusted odds ratios (95% confidence interval) for the prevalence of asthma, rhinitis and eczema comparing those with the highest use of cleaning agents (≥10 points) to those with never/seldom use (0-4 points) were 0.95 (0.56, 1.63), 1.23 (0.82, 1.82) and 0.95 (0.56, 1.63), respectively. For individual cleaning agents, we only found the use of ammonia to be significantly associated with a lower risk of rhinitis [0.60, (0.44, 0.82)]. CONCLUSIONS: There was no indication of an increased prevalence of asthma, rhinitis or eczema among adolescents living in households within the highest category of cleaning agents use.


Assuntos
Detergentes , Exposição Ambiental/estatística & dados numéricos , Hipersensibilidade Respiratória/epidemiologia , Adolescente , Estudos Transversais , Eczema/epidemiologia , Humanos
7.
BMC Public Health ; 19(1): 303, 2019 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-30866869

RESUMO

BACKGROUND: Health information on the dimensions of asthma and allergic conditions in Khuzestan Province, as a major industrial and polluted area in Iran as and the Middle East, is inadequate. This study was performed to measure the prevalence of asthma and other allergic conditions in adults in Khuzestan Province. METHODS: This population-based cross-sectional study was carried out in 17 villages and 27 cities of Khuzestan Province during the years 2017-2018 on 20 to 65 year old respondents. Two-stage cluster sampling was used. The ECRHS (European Community Respiratory Health Survey) questionnaire was completed for individuals with additional questions regarding other allergic conditions. RESULTS: In the study, 5720 questionnaires were distributed of which 5708 were returned. The prevalence of current asthma was 8.5% and that of asthma-like symptoms was 19.0%. The most common symptoms of asthma were nocturnal cough (13.6%), chest tightness (12.3%) and wheezing (13.1%). The prevalence of allergic rhinitis (AR), eczema and airway hyperresponsiveness were 27.2, 10.7, and 38.7%, respectively. The prevalence of current asthma was strongly correlated with age, current location (city, village), and the smoking status of respondents (p < 0.05). CONCLUSION: The prevalence of current asthma and asthma-like symptoms in Khuzestan Province is almost twice as high as in Iran. Given the high prevalence of symptoms of airway hyperresponsiveness in the entire province, it is necessary to take environmental measures to mitigate the emergence of new cases of asthma among the residents. In addition, surveillance studies are necessary to monitor the trends in the prevalence of asthma in this province.


Assuntos
Asma/epidemiologia , Eczema/epidemiologia , Hipersensibilidade Respiratória/epidemiologia , Rinite Alérgica/epidemiologia , Adulto , Idoso , Estudos Transversais , Feminino , Inquéritos Epidemiológicos , Humanos , Irã (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Prevalência , Adulto Jovem
8.
Dermatitis ; 30(1): 62-66, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30640765

RESUMO

BACKGROUND: Atopic dermatitis (AD) associated with respiratory atopy may represent a form of systemic contact dermatitis (SCD), whereby AD flares after ingestion or inhalation of allergens. OBJECTIVE: The aim of the study was to compare the prevalence of positive patch tests to allergens known to cause SCD in AD patients with and without respiratory atopy. METHODS: This is a retrospective study of patients with AD patch tested to 23 allergens known to cause SCD. Positive patch tests were compared between AD patients with and without respiratory atopy, stratified by age and wet or dry work occupation. CONCLUSIONS: Children and adolescents, but not adults, with AD and respiratory atopy were more likely than age-matched AD patients without respiratory atopy to have positive patch tests to these allergens (odds ratio, 2.33; 95% confidence interval, 1.13-4.79). Moreover, AD patients with respiratory atopy and engaging in wet work, but not dry work, occupations were more likely than AD patients without respiratory atopy to have positive patch tests to allergens known to cause SCD (odds ratio, 1.47; 95% confidence interval, 1.05-2.06). Thus, respiratory atopy and wet work are associated with sensitization to allergens known to cause SCD in patients with AD, and patch testing may be valuable in identifying systemic triggers of dermatitis in these patients.


Assuntos
Alérgenos/efeitos adversos , Asma/epidemiologia , Dermatite Alérgica de Contato/epidemiologia , Exposição Ocupacional/estatística & dados numéricos , Rinite Alérgica/epidemiologia , Adolescente , Adulto , Asma/imunologia , Dermatite Alérgica de Contato/etiologia , Dermatite Alérgica de Contato/imunologia , Feminino , Humanos , Hipersensibilidade Imediata/epidemiologia , Hipersensibilidade Imediata/imunologia , Imunização , Masculino , Razão de Chances , Testes do Emplastro , Prevalência , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/imunologia , Estudos Retrospectivos , Rinite Alérgica/imunologia , Adulto Jovem
9.
Rev Mal Respir ; 36(2): 135-141, 2019 Feb.
Artigo em Francês | MEDLINE | ID: mdl-30686558

RESUMO

INTRODUCTION: In tropical Africa, allergies are not well documented. The objective of this work was to evaluate, by two methods, the sensitization to mites in children followed for respiratory allergy. METHODS: Skin prick-test and IgE assay by REAST test with 3 mites: Dermatophagoides pteronyssinus (D. pteronyssinus), Dermatophagoides farinae (D. farinae) and Blomia tropicalis (B. tropicalis) were carried out in children from 3 to 15 years followed up for asthma and/or allergic rhinitis. The positive results of the two tests were compared. RESULTS: Of the 130 (100%) children included, all eligible for the assay, 119 (91.5%) had the prick-test. The mean age and sex ratio (M/F) were 7±1 year, and 1.6. The association of rhinitis and asthma was the most frequent and found in 66 (55.6%). The sensitivity frequencies for the prick-test and assay were respectively 79% versus 36.1% for B. tropicalis, 71.4% versus 33.4% for D. pteronyssinus and 38.7% versus 37.8% for D. farinae. A moderate correlation between mean papule diameter and mean IgE concentration was observed. CONCLUSION: In African tropical environments, dust mite sensitization in children followed for respiratory allergy is frequent, with the order of frequency being: B. tropicalis, D. pteronyssinus, and D. farinae. The prick-test had better sensitivity than the assay for its evaluation.


Assuntos
Dermatophagoides farinae/imunologia , Dermatophagoides pteronyssinus/imunologia , Pyroglyphidae/imunologia , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/epidemiologia , Adolescente , Animais , Antígenos de Dermatophagoides/imunologia , Asma/diagnóstico , Asma/epidemiologia , Asma/etiologia , Benin/epidemiologia , Criança , Pré-Escolar , Feminino , Humanos , Imunoglobulina E/análise , Masculino , Prevalência , Hipersensibilidade Respiratória/imunologia , Rinite Alérgica/diagnóstico , Rinite Alérgica/epidemiologia , Rinite Alérgica/genética , Testes Cutâneos , Clima Tropical
10.
J Med Entomol ; 56(2): 347-352, 2019 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-30462230

RESUMO

House dust mites (HDMs) are the major constituents of house dust (HD). HD and HDM sensitization is well documented worldwide. Storage mite (SM) sensitization is presently lacking from India. The present study evaluated the sensitization of both HDM and SM among 372 allergic rhinitis patients reported to the Allergy and Asthma Research Center of Kolkata metropolitan, India. HD samples were collected from the patients' home and analyzed for the major constituent mites. HD and six constituent mites Dermatophagoides pteronyssinus (DP), Dermatophagoides farina Hughes (Acari: Pyroglyphidae) (DF), Blomia tropicalis (BT), Acarus siro Linnaeus (Acari: Acaridae) (AS), Lepidoglyphus destructor (LD), and Tyrophagus putrescentiae (Schrank) (Acari: Acaridae) (TP) are tested for the allergenic potential through Skin Prick Test (SPT). Three SMs, namely AS, LD, and TP, were newly included in the mite SPT extract for the first time in Kolkata. In total, 330 patients showed significant positive SPT toward any one allergen tested. HD was the major elicitor exhibiting 92.42% response. Individuals of age group 15-40 were the worst sufferers. DF showed the highest sensitization (87.87%) among the dust mites. The SMs also contributed significantly to prove their sensitizing potential. SPT rates for AS, LD, and TP were 33, 25, and 18%, respectively. SPT grades and total Immunoglobulin E (IgE) were positively correlated for each of the allergens. Most of the patients were multi-sensitized (95%) and represented markedly high total IgE levels (>500 IU/ml). Three SMs proved to be significant allergens for the studied population. The sensitization toward these SMs is first time reported from India and can be recommended for inclusion of routine SPT for better outcome in the future.


Assuntos
Acaridae/imunologia , Pyroglyphidae/imunologia , Hipersensibilidade Respiratória/etiologia , Adolescente , Adulto , Animais , Feminino , Humanos , Imunoglobulina E/sangue , Índia/epidemiologia , Masculino , Hipersensibilidade Respiratória/sangue , Hipersensibilidade Respiratória/epidemiologia , Adulto Jovem
11.
J Allergy Clin Immunol ; 143(5): 1752-1759.e6, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30445065

RESUMO

BACKGROUND: Few data exist on the predictors of asthma remission by early adulthood in North America. OBJECTIVE: The predictors of adult asthma remission were determined in a multiethnic population of patients with mild-to-moderate persistent childhood asthma. METHODS: Asthma remission in early adulthood was measured by using 2 definitions: a clinical and a strict definition. Both included normal lung function and the absence of symptoms, exacerbations, and medication use. The strict definition also included normal airways responsiveness. Predictors were identified from 23 baseline measures by using multivariate logistic regression. The probability of remission was modeled by using decision tree analysis. RESULTS: In 879 subjects the mean ± SD baseline age was 8.8 ± 2.1 years, 59.4% were male, and 68.7% were white. By adulthood, 229 (26.0%) of 879 participants were in clinical remission, and 111 (15.0%) of 741 participants were in strict remission. The degree of FEV1/forced vital capacity (FVC) ratio impairment was the largest predictor of asthma remission. More than half of boys and two thirds of girls with baseline FEV1/FVC ratios of 90% or greater were in remission at adulthood. Decreased airways responsiveness was also a predictor for both remission definitions (clinical remission odds ratio, 1.23 [95% CI, 1.09-1.39]; strict remission odds ratio, 1.52 [95% CI, 1.26-1.84]). The combination of normal FEV1/FVC ratio, airways responsiveness, and serum eosinophil count at baseline yielded greater than 80% probability of remission by adulthood. CONCLUSION: A considerable minority of patients with persistent childhood asthma will have disease remission by adulthood. Clinical prognostic indicators of asthma remission, including baseline lung function, can be seen from an early age.


Assuntos
Asma/diagnóstico , Eosinófilos/patologia , Hipersensibilidade Respiratória/diagnóstico , Adulto , Asma/epidemiologia , Criança , Grupos Étnicos , Feminino , Humanos , Contagem de Leucócitos , Masculino , Valor Preditivo dos Testes , Prognóstico , Testes de Função Respiratória , Hipersensibilidade Respiratória/epidemiologia , Resultado do Tratamento , Estados Unidos/epidemiologia
12.
Allergol Immunopathol (Madr) ; 47(4): 350-356, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30578002

RESUMO

INTRODUCTION AND OBJECTIVES: Biological aerosols play a vital role in the interactions between the atmosphere, biosphere, climate and public health and fungal spores are a component with allergic importance. We constructed a database in Castile & Leon (Spain) and carry out molecular-level component-resolved diagnosis to complete the air quality study carried out since 2006 by our aerobiological network (RACYL) to aid clinical diagnosis and treatment. METHODS: We reviewed a database of 19,774 patients (adults and children) with allergic respiratory disease treated in our unit during the last 12 years. We also made a component-resolved diagnosis of the molecules involved in the pathology in a randomly selected population of 150 patients. RESULTS: The dimeric glycoprotein Alt a1 from Alternaria is the most prevalent and most useful allergen in the diagnosis of patients with allergy to fungi in our area (94.4%), followed by enolase Alt a 6 (Alternaria), ribonuclease Asp f 1 of Aspergillus and mannitol dehydrogenase from Cla h 8 (Cladosporium). CONCLUSIONS: Our results have helped determine which spore molecules are most-closely associated with allergies. Molecular analysis will be useful to determine more accurate and useful immunotherapy in these patients.


Assuntos
Alternariose/epidemiologia , Aspergilose/epidemiologia , Hipersensibilidade Respiratória/epidemiologia , Esporos Fúngicos/imunologia , Tinha/epidemiologia , Adulto , Alérgenos/imunologia , Alternaria/imunologia , Antígenos de Fungos/imunologia , Aspergillus/imunologia , Criança , Cladosporium/imunologia , Bases de Dados Factuais , Feminino , Proteínas Fúngicas/imunologia , Humanos , Masculino , Espanha/epidemiologia
13.
Braz J Med Biol Res ; 51(12): e7558, 2018 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-30365724

RESUMO

The association between pet ownership and the development of allergic and respiratory diseases has been the aim of several studies, however, the effects of exposure in adults remain uncertain. The aims of the present study were to investigate the prevalence of asthma and lung function status among dog and cat owners. This cross-sectional study was performed at two universities with students and workers who were allocated into 3 groups according to pet ownership in the previous year: cat owners, dog owners, and no pets (control group). Subjects underwent spirometry, bronchial challenge test with mannitol, skin prick tests, and questionnaires about animal exposures and respiratory symptoms. Control group comprised 125 subjects; cat owner group, 51 subjects; and dog owner group, 140 subjects. Cat owners had increased asthma prevalence (defined by symptoms and positive bronchial challenge test), but no changes in lung function compared to the control group. The dog owner group had lower spirometry values (forced expiratory volume in one second and lower forced vital capacity), but similar asthma prevalence, compared to the control group. In the cat owner group, excess of asthma may have an immunological basis, since we found an association with atopy. Although we did not have endotoxin data from volunteers' households, we postulated that low values of lung function were associated to exposure to endotoxins present in environments exposed to dogs.


Assuntos
Asma/epidemiologia , Asma/etiologia , Gatos , Cães , Pulmão/fisiopatologia , Propriedade/estatística & dados numéricos , Animais de Estimação , Adolescente , Adulto , Alérgenos/efeitos adversos , Análise de Variância , Animais , Asma/fisiopatologia , Brasil/epidemiologia , Criança , Estudos Transversais , Exposição Ambiental/efeitos adversos , Feminino , Volume Expiratório Forçado/fisiologia , Humanos , Masculino , Prevalência , Valores de Referência , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/etiologia , Medição de Risco , Fatores de Risco , Testes Cutâneos , Espirometria , Capacidade Vital/fisiologia , Adulto Jovem
14.
Respir Res ; 19(1): 208, 2018 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-30373568

RESUMO

Rhinovirus (RV) exposure has been implicated in childhood development of wheeze evoking asthma and exacerbations of underlying airways disease. Studies such as the Copenhagen Prospective Studies on Asthma in Childhood (COPSAC) and Childhood Origins of ASThma (COAST) have identified RV as a pathogen inducing severe respiratory disease. RVs also modulate airway hyperresponsiveness (AHR), a key characteristic of such diseases. Although potential factors underlying mechanisms by which RV induces AHR have been postulated, the precise mechanisms of AHR following RV exposure remain elusive.A challenge to RV-related research stems from inadequate models for study. While human models raise ethical concerns and are relatively difficult in terms of subject recruitment, murine models are limited by susceptibility of infection to the relatively uncommon minor group (RV-B) serotypes, strains that are generally associated with infrequent clinical respiratory virus infections. Although a transgenic mouse strain that has been developed has enhanced susceptibility for infection with the common major group (RV-A) serotypes, few studies have focused on RV in the context of allergic airways disease rather than understanding RV-induced AHR. Recently, the receptor for the virulent RV-C CDHR3, was identified, but a dearth of studies have examined RV-C-induced effects in humans.Currently, the mechanisms by which RV infections modulate airway smooth muscle (ASM) shortening or excitation-contraction coupling remain elusive. Further, only one study has investigated the effects of RV on bronchodilatory mechanisms, with only speculation as to mechanisms underlying RV-mediated modulation of bronchoconstriction.


Assuntos
Hipersensibilidade Respiratória/fisiopatologia , Hipersensibilidade Respiratória/virologia , Rhinovirus/isolamento & purificação , Rhinovirus/fisiologia , Poluentes Atmosféricos/efeitos adversos , Animais , Asma/epidemiologia , Asma/fisiopatologia , Asma/virologia , Técnicas de Cocultura , Humanos , Hipersensibilidade Respiratória/epidemiologia
15.
Clin Exp Allergy ; 48(8): 1050-1058, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29700921

RESUMO

BACKGROUND: Various allergenic proteins are produced by house dust mites (HDM). However, the allergenicity and clinical implications of these allergens are unknown. OBJECTIVE: The purpose of this study was to identify allergens in Dermatophagoides farinae and elucidate the sensitization profiles to these in Korean patients suffering from respiratory (allergic rhinitis and/or asthma) and atopic dermatitis symptoms. METHODS: IgE reactivities in sera from 160 HDM allergy patients were analysed by one- and two-dimensional gel electrophoresis and immunoblotting. IgE-reactive components were identified by liquid chromatography-coupled electrospray ionization-tandem mass spectrometry. Nine recombinant mite allergens (Der f 1, Der f 2, Der f 10, Der f 11, Der f 13, Der f 14, Der f 30, Der f 32 and Der f Alt a 10) were produced, and the IgE reactivity in sera to each was determined by ELISAs. RESULTS: Der f 1 and Der f 2 were recognized by IgE in serum samples from 88.1% and 78.1% of all patients, respectively. Patients with respiratory allergies were mainly sensitized to these major allergens, whereas patients with atopic dermatitis symptoms showed polysensitization to major and minor allergen components (including Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10). CONCLUSIONS: Patients with respiratory allergic disease sensitize to major allergen components of HDM. Those with atopic dermatitis were sensitized to a broader range of minor allergen components of HDM (Der f 11, Der f 13, Der f 14, Der f 32 and Der f Alt a 10).


Assuntos
Alérgenos/imunologia , Antígenos de Dermatophagoides/imunologia , Dermatite Atópica/imunologia , Pyroglyphidae/imunologia , Hipersensibilidade Respiratória/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Especificidade de Anticorpos/imunologia , Biomarcadores , Criança , Dermatite Atópica/complicações , Dermatite Atópica/diagnóstico , Dermatite Atópica/epidemiologia , Diagnóstico Diferencial , Feminino , Humanos , Imunização , Imunoensaio , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Masculino , Pessoa de Meia-Idade , Vigilância em Saúde Pública , República da Coreia/epidemiologia , Hipersensibilidade Respiratória/complicações , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/epidemiologia , Testes Cutâneos , Adulto Jovem
16.
Allergol. immunopatol ; 46(2): 112-118, mar.-abr. 2018. tab
Artigo em Inglês | IBECS | ID: ibc-172168

RESUMO

Background: The objective was to estimate the incidence of asthma in young adults from 13-15 years old to 23-25 years old, and associated factors. Methods: In 2012, a population-based prospective cohort study was carried out in Castellon from the cohort who had participated in the International Study of Asthma and Allergy in Childhood in 1994 and 2002. A telephone survey was undertaken using the same questionnaires. A new case of asthma was defined as a participant free of the disease in 2002 who suffered asthma, was diagnosed with asthma, or took medications against asthma based on self-report from 2002 to 2012. Results: The mean age of participants was 24.9 ± 0.6 with a follow-up of 79.1%. Asthma cumulative incidence was 3.4%: 44 new cases occurred among 1280 participants. The incidence was higher in females than males with relative risk (RR) =2.02 (95% confidence interval [CI] 1.1-3.8). A significant decrease of asthma incidence density was observed (8.2 cases to 3.5 cases per 1000 person/year). Factors associated with the incidence of asthma were allergic rhinitis (RR = 4.05; 95% CI 1.7-9.6), bronchitis (RR = 2.13; 95% CI 1.0-4.5), mother's age at time of birth (RR=0.87; 95% CI 0.8-0.9) and a pet other than a dog or cat (RR = 0.42; 95% CI 0.2-0.9). For gender, some variations in the risk factors were observed. Conclusions: A significant decrease in the incidence of asthma was observed. Several risk and protective factors were found (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Asma/epidemiologia , Hipersensibilidade Respiratória/epidemiologia , Antiasmáticos/uso terapêutico , Estudos de Coortes , Estudos Prospectivos , Autorrelato , Fatores de Risco
17.
Allergol. immunopatol ; 46(2): 119-126, mar.-abr. 2018. tab
Artigo em Inglês | IBECS | ID: ibc-172169

RESUMO

Background: The presence of atopy is considered as a risk factor for severe respiratory symptoms in children. The objective of this study was to examine the effect of atopy on the course of disease in children hospitalised with viral pneumonia. Methods: Children between the ages of 1 and 6 years hospitalised due to viral pneumonia between the years of 2013 and 2016 were included to this multicentre study. Patients were classified into two groups as mild-moderate and severe according to the course of pneumonia. Presence of atopy was evaluated with skin prick tests. Groups were compared to evaluate the risk factors associated with severe viral pneumonia. Results: A total of 280 patients from nine centres were included in the study. Of these patients, 163 (58.2%) were male. Respiratory syncytial virus (29.7%), Influenza A (20.5%), rhinovirus (18.9%), adenovirus (10%), human metapneumovirus (8%), parainfluenza (5.2%), coronavirus (6%), and bocavirus (1.6%) were isolated from respiratory samples. Eighty-five (30.4%) children had severe pneumonia. Atopic sensitisation was found in 21.4% of the patients. Ever wheezing (RR: 1.6, 95% CI: 1.1-2.4), parental asthma (RR: 1.5, 95% CI: 1.1-2.2), other allergic diseases in the family (RR: 1.8, 95% CI: 1.2-2.9) and environmental tobacco smoke (RR: 1.6, 95% CI: 1.1-3.5) were more common in the severe pneumonia group. Conclusions: When patients with mild-moderate pneumonia were compared to patients with severe pneumonia, frequency of atopy was not different between the two groups. However, parental asthma, ever wheezing and environmental tobacco smoke exposure are risk factors for severe viral pneumonia in children (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Hipersensibilidade Imediata/complicações , Pneumonia Viral/complicações , Fatores de Risco , Criança Hospitalizada/estatística & dados numéricos , Asma/epidemiologia , Rinite Alérgica/epidemiologia , Hipersensibilidade Respiratória/epidemiologia , Estudos Prospectivos , Testes Cutâneos
18.
Allergol. immunopatol ; 46(2): 160-166, mar.-abr. 2018. tab, graf
Artigo em Inglês | IBECS | ID: ibc-172175

RESUMO

Background: This paper sought to evaluate individual exposure to air pollution by quantifying the carbon in alveolar macrophages (AMs) and its relationship to lung function. We also examined the proximity of participants' residences to the Presidente Dutra highway (PDH) in adolescents with asthma from Taubaté, São Paulo, Brazil. Methods: This descriptive study examined fifty 13- to 14-year-old adolescents with asthma identified by the International Study of Asthma and Allergies in Childhood (ISAAC) in Taubaté. These adolescents underwent spirometry and sputum induction via the inhalation of 3% hypertonic saline (HSS). Sputum was collected after each nebulisation, and forced expiratory flow in one second (FEV1) was measured. The collected sputum was stored and transported to the laboratory; it was then processed and analysed for ultrafine particles (≤100 nm). This analysis was correlated with the residence location and FEV1 of each adolescent. Results: A total of 39 adolescents completed the study. The comparison of the carbon fraction within macrophages (CA/MA) showed no differences according to residence in relation to the PDH (p=0.758). After adjustment, a mixed linear model with FEV1 as the dependent variable and CA/MA, location, and evaluation condition as the predictors found that the interactions among the variables were not significant. Conclusions:T he amount of carbon present within the AMs of adolescents with asthma was not correlated with either lung function or residence location. Evaluations of the topograghy and local climatic conditions in Taubaté should be considered in future studies (AU)


No disponible


Assuntos
Humanos , Adolescente , Poluição do Ar/análise , Material Particulado/isolamento & purificação , Asma/epidemiologia , Fuligem/isolamento & purificação , Poluição do Ar/efeitos adversos , Fenômenos Fisiológicos Respiratórios , Hipersensibilidade Respiratória/epidemiologia , Fuligem/efeitos adversos , Geografia Médica
19.
PLoS One ; 13(3): e0194775, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29566093

RESUMO

The immunological profiles of human specific IgE (sIgE) and specific IgG4 (sIgG4) vary by genetic predisposition, living conditions in different geographical locations and patient's age. The aim of our study was to analyze sIgE and sIgG4 patterns and their age-dependent changes in patients from the Moscow region. For identifying sIgE and sIgG4 profiles the blood samples from 513 patients aged 6 months to 17 years who were showing symptoms of allergic diseases were analyzed using microarrays containing 31 allergens. The highest sIgE prevalence was observed for birch pollen (32%) among pollen allergens, cat dander (24%) among indoor allergens, and egg whites (21%) among food allergens. The most common sIgG4 response was developed toward egg whites (80% of patients). Age-related elevation was identified for patients with increased sIgE to pollen allergens and indoor allergens (cat or dog dander and house dust mites). For each allergen, the proportion of cases with significant levels of sIgG4 appeared to increase with patients' age. The data on allergen-specific sIgE and sIgG4 prevalence show both general trends and some local special aspects that are indicative for the Moscow region. This information should be useful in terms of epidemiology of allergic diseases.


Assuntos
Alérgenos/análise , Hipersensibilidade Alimentar/diagnóstico , Hipersensibilidade Alimentar/epidemiologia , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/epidemiologia , Adolescente , Alérgenos/classificação , Testes de Provocação Brônquica/métodos , Testes de Provocação Brônquica/estatística & dados numéricos , Criança , Pré-Escolar , Feminino , Hipersensibilidade Alimentar/imunologia , Hipersensibilidade Alimentar/metabolismo , Humanos , Lactente , Recém-Nascido , Masculino , Análise em Microsséries , Moscou/epidemiologia , Hipersensibilidade Respiratória/imunologia , Hipersensibilidade Respiratória/metabolismo , Federação Russa/epidemiologia
20.
Thorax ; 73(12): 1170-1173, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-29563159

RESUMO

In the non-selected birth cohort Tucson Children's Respiratory Study, early sensitisation to Alternaria was associated with increased airway hyper-responsiveness (AHR) into adult life among non-asthmatics. The increase in AHR was of a similar magnitude to that seen for Alternaria sensitised asthmatics and was primarily evident among those who were overweight or obese. In contrast, there was no significant association between early sensitisation to aeroallergens other than Alternaria and AHR among non-asthmatics. Why this group of Alternaria sensitised individuals without asthma demonstrated increased AHR of a magnitude similar to asthmatics is unknown and requires further investigation.


Assuntos
Alternaria/imunologia , Antígenos de Fungos , Asma/imunologia , Hipersensibilidade Respiratória/epidemiologia , Hipersensibilidade Respiratória/imunologia , Adolescente , Adulto , Arizona/epidemiologia , Asma/fisiopatologia , Criança , Volume Expiratório Forçado , Humanos , Obesidade/epidemiologia , Hipersensibilidade Respiratória/fisiopatologia , Testes Cutâneos , Espirometria , Adulto Jovem
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