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1.
J Surg Res ; 281: 97-103, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36137358

RESUMO

INTRODUCTION: Cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) are accepted treatment options for patients with cancers associated with the abdominal region. Currently, there are insufficient data that evaluate the effectiveness of these therapeutic options in young patients who have peritoneal metastasis (PM) originating from colorectal cancer. METHODS: In this investigation, we evaluated perioperative data, clinicopathological manifestations, and survival consequences in 46 younger (≤50 y) patients who underwent CRS and HIPEC therapy between 2017 June and 2019 June and then compared these data with 94 older patients (aged >50 y). RESULTS: Compared with older patients, younger patients had a higher synchronous PM incidence (78.3% versus 51.1%, P < 0.001) and were more likely to exhibit signet ring histology and mucinous (29.8% versus 60.9%; P < 0.001). The cancer-specific survival rates after CRS and HIPEC treatment were similar in both age sets. Multivariate Cox regression revealed that mucinous/signet adenocarcinoma (hazard ratio 2.20, 95% confidence interval 1.02-4.74; P = 0.044) and rectal origin (hazard ratio 2.51, 95% confidence interval 1.11-5.67; P = 0.027) were independent risk factors for a lower cancer-specific survival rate. CONCLUSIONS: Younger (age ≤50 y) patients who have PM of colorectal cancer origin often present synchronous PM, which is less commonly observed in older patients. Tumors in younger patients are more aggressive; however, post-CRS and HIPEC treatment, the benefits are similar to those observed in older cohorts.


Assuntos
Neoplasias Colorretais , Hipertermia Induzida , Neoplasias Peritoneais , Humanos , Idoso , Procedimentos Cirúrgicos de Citorredução , Neoplasias Peritoneais/terapia , Neoplasias Peritoneais/secundário , Quimioterapia Intraperitoneal Hipertérmica , Quimioterapia do Câncer por Perfusão Regional , Neoplasias Colorretais/patologia , Taxa de Sobrevida , Terapia Combinada , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Prognóstico
2.
Carbohydr Polym ; 300: 120246, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36372507

RESUMO

This work represents a biocompatible magnetic nanobiocomposite prepared by the composition of chitosan (CS) hydrogel, silk fibroin (SF), graphene oxide (GO), and Fe3O4 NPs. Terephthaloyl thiourea was applied as a cross-linking agent to cross-link the CS strings. The CS hydrogel/SF/GO/Fe3O4 nanobiocomposite with many characteristics, such as high structural uniformity, thermal stability, biocompatibility, and stability in an aqueous solution. Various characteristics of this novel magnetic nanobiocomposite were distinguished by FT-IR, EDX, FE-SEM, XRD, TGA, and VSM analysis. The FE-SEM images were taken to evaluate the size distribution of the magnetic nanoparticles (MNPs) between 39.9 and 73.3 nm as well. The performance of the prepared nanobiocomposite was assessed by the magnetic fluid hyperthermia process. Under the alternating magnetic field (AMF), the mean value of the specific absorption rate (SAR) was determined at 43.15 w/g.


Assuntos
Quitosana , Fibroínas , Hipertermia Induzida , Quitosana/química , Fibroínas/química , Hidrogéis/química , Espectroscopia de Infravermelho com Transformada de Fourier , Fenômenos Magnéticos
3.
Clin Adv Hematol Oncol ; 20(11): 673-682, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36331405

RESUMO

Gastric cancer (GC) with peritoneal carcinomatosis (PC) progresses rapidly and has historically dismal survival rates. Given the aggressive tumor biology and poor survival outcomes of patients with GC/PC, additional treatments beyond systemic chemotherapy are needed. Cytoreductive surgery and intraperitoneal chemotherapy have been effective management options for peritoneal surface malignancies, with increasing data to support their use in GC/PC. This review highlights the evolution of the surgical treatment of GC/PC, and discusses critical studies supporting the role of cytoreductive surgery, appropriate patient selection, and various methods in the delivery of intraperitoneal chemotherapy for patients with GC/PC.


Assuntos
Hipertermia Induzida , Neoplasias Peritoneais , Neoplasias Gástricas , Humanos , Procedimentos Cirúrgicos de Citorredução/métodos , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/cirurgia , Hipertermia Induzida/métodos , Neoplasias Peritoneais/tratamento farmacológico , Neoplasias Peritoneais/cirurgia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
4.
J Adv Res ; 41: 23-38, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36328751

RESUMO

INTRODUCTION: The advanced features of plasmonic nanomaterials enable initial high accuracy detection with different therapeutic intervention. Computational simulations could estimate the plasmonic heat generation with a high accuracy and could be reliably compared to experimental results. This proposed combined theoretical-experimental strategy may help researchers to better understand other nanoparticles in terms of plasmonic efficiency and usability for future nano-theranostic research. OBJECTIVES: To develop innovative computationally-driven approach to quantify any plasmonic nanoparticles photothermal efficiency and effects before their use as therapeutic agents. METHODS: This report introduces drug free plasmonic silver triangular nanoprisms coated with polyvinyl alcohol biopolymer (PVA-SNT), for in vivo photoacoustic imaging (PAI) guided photothermal treatment (PTT) of triple-negative breast cancer mouse models. The synthesized PVA-SNT nanoparticles were characterized and a computational electrodynamic analysis was performed to evaluate and predict the optical and plasmonic photothermal properties. The in vitro biocompatibility and in vivo tumor abalation study was performed with MDA-MB-231 human breast cancer cell line and in nude mice model. RESULTS: The drug free 140 µg∙mL-1 PVA-SNT nanoparticles with 1.0 W∙cm-2 laser irradiation for 7 min proved to be an effective and optimized theranostic approach in terms of PAI guided triple negative breast cancer treatment. The PVA-SNT nanoparticles exhibits excellent biosafety, photostability, and strong efficiency as PAI contrast agent to visualize tumors. Histological analysis and fluorescence-assisted cell shorter assay results post-treatment apoptotic cells, more importantly, it shows substantial damage to in vivo tumor tissues, killing almost all affected cells, with no recurrence. CONCLUSION: This is a first complete study on computational simulations to estimate the plasmonic heat generation followed by drug free plasmonic PAI guided PTT for cancer treatment. This computationally-driven theranostic approach demonstrates an innovative thought regarding the nanoparticles shape, size, concentration, and composition which could be useful for the prediction of photothermal heat generation in precise nanomedicine applications.


Assuntos
Hipertermia Induzida , Neoplasias , Técnicas Fotoacústicas , Animais , Humanos , Camundongos , Nanomedicina Teranóstica/métodos , Fototerapia/métodos , Prata/uso terapêutico , Técnicas Fotoacústicas/métodos , Terapia Fototérmica , Hipertermia Induzida/métodos , Camundongos Nus , Neoplasias/tratamento farmacológico
5.
Biointerphases ; 17(6): 061004, 2022 11 11.
Artigo em Inglês | MEDLINE | ID: mdl-36368941

RESUMO

Magnetic nanoparticle (MNP) induced magnetic hyperthermia has been demonstrated as a promising technique for the treatment of brain tumor. However, lower heating efficiency resulting from low intratumoral accumulation of magnetic nanomaterials is still one of the significant limitations for their thermotherapeutic efficacy. In this study, we have designed a nanobubble structure with MNPs decorated on the shell, which leads to the improvement of magnetocaloric performance under an alternating magnetic field. First, the phospholipid coupled with MNPs as the shell to be self-assembled magnetic nanobubbles (MNBs) was fabricated by a temperature-regulated repeated compression self-assembly approach. Then, the optimal magnetic heating concentration, electric current parameters for producing the magnetic field, and the number of magnetic heating times were investigated for tuning the better magnetoenergy conversion. Finally, the well-defined geometrical orientation of MNPs on the nanobubble structure enhanced hypothermia effect was investigated. The results demonstrate that the MNBs could promote the endocytosis of magnetic nanoparticles by glioma cells, resulting in better therapeutic effect. Therefore, the controlled assembly of MNPs into well-defined bubble structures could serve as a new hyperthermia agent for tumor therapy.


Assuntos
Glioma , Hipertermia Induzida , Nanopartículas de Magnetita , Humanos , Nanopartículas de Magnetita/uso terapêutico , Nanopartículas de Magnetita/química , Hipertermia Induzida/métodos , Glioma/terapia , Fenômenos Magnéticos
6.
ACS Appl Bio Mater ; 5(11): 5386-5393, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36350576

RESUMO

Monodispersed core@shell γ-Fe2O3@MnxOy nanoparticles have been prepared through thermolysis of iron and manganese oleate. Further, these prepared nanoparticles are coated with biocompatible substances such as silica and polyethylene glycol. These particles are highly biocompatible for different cell lines such as normal and cancer cell lines. The nanoparticles are used as hyperthermia agents, and successful hyperthermia treatment in cancer cells is carried out. As compared to γ-Fe2O3@SiO2, γ-Fe2O3@MnxOy@SiO2 shows the enhanced killing of cancer cells through hyperthermia. In order to make them potential candidates for targeting to cancer cells, folic acid (FA) is tagged to the nanoparticles. Fluorescein isothiocyanate (FITC) is also tagged onto these nanoparticles for imaging. The developed γ-Fe2O3@MnxOy@SiO2 nanoparticle can act as a single entity for therapy through AC magnetic field, imaging through FITC and targeting through folic acid simultaneously. This is the first report on this material, which is highly biocompatible for hyperthermia, imaging, and targeting.


Assuntos
Hipertermia Induzida , Nanopartículas , Humanos , Dióxido de Silício , Fluoresceína-5-Isotiocianato , Hipertermia , Nanopartículas/uso terapêutico , Ácido Fólico , Fluoresceína
7.
Neuro Oncol ; 24(Suppl 6): S42-S51, 2022 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-36322099

RESUMO

Magnetic resonance imaging-guided laser interstitial thermal therapy (LITT) is an ablative procedure using heat from a laser to provide cytoreduction in tissue. It is a minimally invasive procedure that has been used in intracranial pathologies such as high-grade gliomas, metastatic lesions, epilepsy, and other lesions. While LITT may offer a more acceptable complication profile compared to open surgery, the role of laser therapy for intracranial lesions in current treatment paradigms continues to evolve. This review will focus on the background and application of LITT, the current evidence for its use, and future directions for the technology.


Assuntos
Neoplasias Encefálicas , Glioma , Hipertermia Induzida , Terapia a Laser , Humanos , Neoplasias Encefálicas/cirurgia , Glioma/cirurgia , Terapia a Laser/métodos , Hipertermia Induzida/métodos , Imageamento por Ressonância Magnética , Lasers
8.
ACS Appl Mater Interfaces ; 14(45): 50557-50568, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36322879

RESUMO

Single ionizing radiation at a tolerable dose is ineffectual in eliminating malignancies but readily generates harmful effects on surrounding normal tissues. Herein, we intelligently fabricated novel wolfram-doped polypyrrole (WPPy) through a simple oxidative polymerization method with WCl6 as an oxidizing catalyst, which possessed good biocompatibility, high photothermal conversion, and intensive radiosensitivity capacities to concurrently serve as a photothermal reagent and a radiosensitizer for hyperthermia-synergized radiotherapy (RT) against a malignant tumor. In comparison with traditional polypyrrole without noble metal doping, the innovative introduction of WCl6 not only successfully launched the polymerization of a pyrrole monomer but also endowed WPPy with additional radiosensitization. More importantly, after further decoration with an active targeted component (SP94 polypeptide), the obtained WPPy@SP94 significantly increased tumor internalization and accumulation in vitro and in vivo and induced obvious DNA damage as well as robust ROS generation under X-ray irradiation, which meanwhile synergized with strong photonic hyperthermia to effectively inhibit tumor growth by single drug injection. Moreover, such biocompatible WPPy@SP94 showed negligible adverse effects on normal cells and tissues. WPPy@SP94 developed in this study not only expands the category of polypyrrole chemical syntheses but also sheds light on WPPy@SP94-based radiosensitizers for cancer RT.


Assuntos
Hipertermia Induzida , Neoplasias , Radiossensibilizantes , Humanos , Polímeros , Pirróis , Tungstênio , Neoplasias/radioterapia , Radiossensibilizantes/farmacologia , Hipertermia , Linhagem Celular Tumoral
10.
Int J Hyperthermia ; 39(1): 1421-1439, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36396127

RESUMO

BACKGROUND: A necessary precondition for a successful microwave hyperthermia (HT) treatment delivered by phased arrays is the ability of the HT applicator to selectively raise the temperature of the entire tumor volume. SAR-based treatment plan (HTP) optimization methods exploit the correlation between specific absorption rate (SAR) and temperature increase in order to determine the set of steering parameters for optimal focusing, while allowing for lower model complexity. Several cost functions have been suggested in the past for this optimization problem. However, their correlation with high and homogeneous tumor temperatures remains sub-optimal in many cases. Previously, we proposed the hot-to-cold spot quotient (HCQ) as a novel cost function for SAR-based HTP optimization and showed its potential to address these issues. MATERIALS AND METHODS: In this work, we validate the HCQ on a standard ESHO patient repository within single and multi-frequency contexts. We verify its correlation with clinical SAR and temperature indexes, and compare it to HTPs obtained using a commonly accepted cost-function for SAR-based HTP (hot-spot to target quotient, HTQ). RESULTS AND DISCUSSION: The results show that low HCQ values produce better SAR (TC50, TC75) and temperature metrics (T50, T90) than HTQ in most patient models and frequency settings. For the deep-seated tumors, the correlation between the clinical indicators and 1/HCQ is more favorable than the correlation exhibited by 1/HTQ. CONCLUSION: The validation confirms the ability of HCQ to promote target coverage and hot-spot suppression in SAR-based HTP optimization, resulting in higher SAR and temperature indexes for deep-seated tumors.


Assuntos
Hipertermia Induzida , Neoplasias , Humanos , Micro-Ondas/uso terapêutico , Hipertermia Induzida/métodos , Hipertermia , Temperatura Baixa , Neoplasias/terapia
11.
Zhonghua Wei Chang Wai Ke Za Zhi ; 25(11): 947-954, 2022 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-36396367

RESUMO

Peritoneal metastasis is one of the common metastasis of gastrointestinal malignancy. Intraperitoneal chemotherapy (including hyperthermic intraperitoneal chemotherapy and intraoperative intraperitoneal chemotherapy) not only can effectively increase the peritoneal drug concentration, but also can reduce side effects of systemic chemotherapy. It can significantly prolong the long term survival of patients with peritoneal metastasis and improve the quality of life. In order to standardize the popularization and application of intraperitoneal chemotherapy, relevant professional committees have formulated expert consensus on intraperitoneal chemotherapy. However, there is no systematic guidance on the prevention and treatment of related complications of intraperitoneal chemotherapy. Under the guidance of the Complications Management Committee of the Colorectal Cancer Professional Committee of the Chinese Medical Association and the Colorectal Cancer Professional Committee of the Chinese Anti-cancer Association, we organized domestic experts in relevant fields to formulate this consensus according to the procedural specifications and relevant literature reports. This consensus aims to summarize the causes of common complications of intraperitoneal chemotherapy such as pneumonia, intraperitoneal hemorrhage, fever, peritonitis, ileus, intestinal dysfunction, anastomotic bleeding, anastomotic leakage, leakage or infection of perfusion tube, nausea and vomiting, myelosuppression, hepatic or nephritic dysfunction. After repeatedly soliciting the opinions of domestic authoritative experts and their discussion and modification, a consensus was formed to provide effective reference for the prevention and treatment of complications.


Assuntos
Neoplasias Colorretais , Neoplasias Gastrointestinais , Hipertermia Induzida , Neoplasias Peritoneais , Humanos , Neoplasias Peritoneais/secundário , Consenso , Qualidade de Vida , Terapia Combinada , Neoplasias Gastrointestinais/terapia , Perfusão , Neoplasias Colorretais/cirurgia , China
12.
Artigo em Inglês | MEDLINE | ID: mdl-36361500

RESUMO

Throughout history a variety of therapeutic tools have been studied as possible enhancers of sports activities. This study proposes the use of Capacitive-Resistive Electric Transfer (CRET) as a performance booster to paralympic athletes, specifically those belonging to the Spanish Paralympic swimming committee. The study was a randomized, single-blind, and observer-blind, crossover clinical trial. Six athletes were randomly assigned to three groups: one treated with CRET (A); a placebo group (B) and a control group (C). The CRET group attended a twenty-minute session before being subjected to pool trials at distances of 50 and 100 m at maximum performance. Measurements were in two dimensions: time in seconds and the Borg scale for perceived exertion. Comparisons between groups were made with respect to distance and the main variables. In the case of perceived exertion, no significant changes were observed in any of the distances; however, in the case of the time variable, a significant difference was observed between Group A vs. Personal Record at 100 m distance (76.3 ± 6.8 vs. 68.4 ± 3.3). The proposed protocol and level of hyperthermia applied suggest refusal of CRET use for the 100-m distance a few minutes before sports practice. Our analysis suggests the need to modify the presented protocol. ClinicalTrials.gov identifier under NCT number: NCT04336007.


Assuntos
Desempenho Atlético , Hipertermia Induzida , Humanos , Método Simples-Cego , Natação , Atletas
13.
Int J Mol Sci ; 23(21)2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36361892

RESUMO

Metal nanoparticles (NPs), particularly gold nanorods (AuNRs), appear as excellent platforms not only to transport and deliver bioactive cargoes but also to provide additional therapeutic responses for diseased cells and tissues and/or to complement the action of the carried molecules. In this manner, here, we optimized a previous developed metal-based nanoplatform composed of an AuNR core surrounded by a polymeric shell constructed by means of the layer-by-layer approach, and in which very large amounts of the antineoplasic drug doxorubicin (DOXO) in a single loading step and targeting capability thanks to an outer hyaluronic acid layer were incorporated by means of an optimized fabrication process (PSS/DOXO/PLL/HA-coated AuNRs). The platform retained its nanometer size with a negative surface charge and was colloidally stable in a range of physiological conditions, in which only in some of them some particle clustering was noted with no precipitation. In addition, the dual stimuli-responsiveness of the designed nanoplatform to both endogenous proteases and external applied light stimuli allows to perfectly manipulate the chemodrug release rates and profiles to achieve suitable pharmacodynamics. It was observed that the inherent active targeting abilities of the nanoplatfom allow the achievement of specific cell toxicity in tumoral cervical HeLa cells, whilst healthy ones such as 3T3-Balb fibroblast remain safe and alive in agreement with the detected levels of internalization in each cell line. In addition, the bimodal action of simultaneous chemo- and photothermal bioactivity provided by the platform largely enhances the therapeutic outcomes. Finally, it was observed that our PSS/DOXO/PLL/HA-coated AuNRs induced cell mortality mainly through apoptosis in HeLa cells even in the presence of NIR light irradiation, which agrees with the idea of the chemo-activity of DOXO predominating over the photothermal effect to induce cell death, favoring an apoptotic pathway over necrosis for cell death.


Assuntos
Hipertermia Induzida , Nanotubos , Neoplasias , Humanos , Ouro , Células HeLa , Doxorrubicina/farmacologia , Sistemas de Liberação de Medicamentos , Fototerapia
14.
J Mater Chem B ; 10(44): 9249-9257, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36321642

RESUMO

Incomplete tumor ablation and subsequent tumor metastasis usually occur during photothermal anti-tumor processes. The combination of photothermal and immunotherapy has proven to be a promising method to conquer technical challenges. Inhibiting the programmed death ligand-1 (PD-L1)/programmed cell death protein 1 (PD-1) immune pathway represents one of the most successful immunotherapy strategies. Whereas, the PD-L1 expression level significantly differs, leading to a relatively low response rate to the immune checkpoint blockade (ICB) approaches. Therefore, improving the expression level of PD-L1 becomes one potential method to enhance the response rate. Herein, NIH 3T3 cells were educated to steadily express PD-1 protein. Furthermore, the synthesized molybdenum nitride was then coated with PD-1 protein-modified cytomembrane, which endows it with immune checkpoint blocking capability. Moreover, under the irradiation of near-infrared light, the local mild heat released from the molybdenum nitride causes the apoptosis of tumor cells. More importantly, the elevated temperature simultaneously helps elevate the expression level of PD-L1, further enhancing the response rate of ICB. Finally, the PD-1 cytomembrane coatings interact with the upregulated PD-L1, leading to the activation of the immune system. In summary, we confirmed that the PD-1 protein-coated molybdenum nitride could synergistically ablate tumors and avoid metastasis.


Assuntos
Neoplasias da Mama , Hipertermia Induzida , Camundongos , Animais , Humanos , Feminino , Antígeno B7-H1/metabolismo , Receptor de Morte Celular Programada 1/metabolismo , Neoplasias da Mama/terapia , Molibdênio/farmacologia , Imunoterapia , Fatores Imunológicos
16.
Sensors (Basel) ; 22(22)2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36433476

RESUMO

The characterization of nanoparticles is crucial in several medical applications, such as hyperthermic therapy, which heats superparamagnetic nanoparticles with an external electromagnetic field. The knowledge of heating ability (magnetic losses) in AC magnetic field frequency function allows for selecting the optimal excitation. A hybrid system for the characterization of superparamagnetic nanoparticles was designed and tested. The proposed setup consists of an excitation coil and two sensing probes: calorimetric and magnetic. The measurements of the imaginary part of the complex magnetic susceptibility of superparamagnetic nanoparticles are possible in the kilohertz range. The system was verified using a set of nanoparticles with different diameters. The measurement procedure was described and verified. The results confirmed that an elaborated sensor system and measuring procedures could properly characterize the magnetic characteristics of nanoparticles. The main advantage of this system is the ability to compare both characteristics and confirm the selection of optimal excitation parameters.


Assuntos
Hipertermia Induzida , Nanopartículas , Magnetismo , Hipertermia Induzida/métodos , Campos Magnéticos , Nanopartículas Magnéticas de Óxido de Ferro
17.
Int J Mol Sci ; 23(22)2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36430620

RESUMO

Superparamagnetic nanoparticles are of high interest for therapeutic applications. In this work, nanoparticles of calcium-doped manganese ferrites (CaxMn1-xFe2O4) functionalized with citrate were synthesized through thermally assisted oxidative precipitation in aqueous media. The method provided well dispersed aqueous suspensions of nanoparticles through a one-pot synthesis, in which the temperature and Ca/Mn ratio were found to influence the particles microstructure and morphology. Consequently, changes were obtained in the optical and magnetic properties that were studied through UV-Vis absorption and SQUID, respectively. XRD and Raman spectroscopy studies were carried out to assess the microstructural changes associated with stoichiometry of the particles, and the stability in physiological pH was studied through DLS. The nanoparticles displayed high values of magnetization and heating efficiency for several alternating magnetic field conditions, compatible with biological applications. Hereby, the employed method provides a promising strategy for the development of particles with adequate properties for magnetic hyperthermia applications, such as drug delivery and cancer therapy.


Assuntos
Hipertermia Induzida , Nanopartículas , Manganês , Cálcio , Hipertermia Induzida/métodos , Nanopartículas/química , Cálcio na Dieta , Campos Magnéticos , Estresse Oxidativo
18.
Int J Mol Sci ; 23(22)2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36430744

RESUMO

Photothermal therapy is a treatment technique that has attracted attention as an alternative to conventional surgical techniques. It is based on the photothermal effect, wherein light energy is converted into thermal energy, and facilitates rapid recovery after treatment. This study employed various laser irradiation conditions and presented conditions with the optimal treatment effects through a numerical analysis based on heat transfer. A skin layer comprising four stages containing squamous cell carcinoma was targeted, and the treatment effect was confirmed by varying the heating conditions of the laser and volume fraction of gold nanoparticles. The therapeutic effect was confirmed through both the apoptosis retention ratio, which quantitatively estimated the degree of maintenance of the apoptosis temperature range within the tumor, and the thermal hazard retention value, which quantitatively calculates the amount of thermal damage to the surrounding normal tissues. Finally, the optimal treatment conditions were determined based on the laser intensity, cooling time ratio, and volume fraction of injected gold nanoparticles through numerical analysis.


Assuntos
Hipertermia Induzida , Nanopartículas Metálicas , Terapia Fototérmica , Ouro/uso terapêutico , Nanopartículas Metálicas/uso terapêutico , Lasers , Hipertermia Induzida/métodos
19.
Nano Lett ; 22(22): 8852-8859, 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36346801

RESUMO

Hyperthermia of superparamagnetic nanoparticles driven by Néel relaxation in an alternating magnetic field (AMF) has been studied in biomedical areas; however, Brownian motion, induced by another magnetic relaxation mechanism, has not been explored extensively despite its potential in intracellular mechanoresponsive applications. We investigated whether superparamagnetic cage-shaped iron oxide nanoparticles (IO-nanocages), previously demonstrated to carry payloads inside their cavities for drug delivery, can generate Brownian motion by tuning the nanoparticle size at 335 kHz AMF frequency. The motivation of this work is to examine the magnetically driven Brownian motion for the delivery of nanoparticles allowing escape from endosomes before digestion in lysosomes and efficient delivery of siRNA cargoes to the cytoplasm. Superconducting quantum interference device (SQUID) measurements reveal the nanocage size dependence of Brownian relaxation, and a magnetic Brownian motion of 20 nm IO-nanocages improved the efficiency of siRNA delivery while endosomal membranes were observed to be compromised to release IO-nanocages in AMFs during the delivery process.


Assuntos
Compostos Férricos , Hipertermia Induzida , RNA Interferente Pequeno/genética , Campos Magnéticos , Movimento (Física)
20.
ACS Nano ; 16(11): 18376-18389, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36355037

RESUMO

Currently, one of the main reasons for the ineffectiveness of tumor treatment is that the abnormally high tumor interstitial pressure (TIP) hinders the delivery of drugs to the tumor center and promotes intratumoral cell survival and metastasis. Herein, we designed a "nanomotor" by in situ growth of Ag2S nanoparticles on the surface of ultrathin WS2 to fabricate Z-scheme photocatalytic drug AWS@M, which could rapidly enter tumors by splitting water in interstitial liquid to reduce TIP, along with O2 generation. Moreover, the O2 would be further converted to reactive oxygen species (ROS), accompanied by increased local temperature of tumors, and the combination of ROS with thermotherapy could eliminate the deep tumor cells. Therefore, the "nanomotor'' could effectively reduce the TIP levels of cervical cancer and pancreatic cancer (degradation rates of 40.2% and 36.1%, respectively) under 660 nm laser irradiation, further enhance intratumor drug delivery, and inhibit tumor growth (inhibition ratio 95.83% and 87.61%, respectively), and the related mechanism in vivo was explored. This work achieves efficiently photocatalytic water-splitting in tumor interstitial fluid to reduce TIP by the nanomotor, which addresses the bottleneck problem of blocking of intratumor drug delivery, and provides a general strategy for effectively inhibiting tumor growth.


Assuntos
Hipertermia Induzida , Nanopartículas , Neoplasias , Humanos , Espécies Reativas de Oxigênio/metabolismo , Sistemas de Liberação de Medicamentos , Nanopartículas/uso terapêutico , Neoplasias/tratamento farmacológico , Água , Linhagem Celular Tumoral
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