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1.
Clin Anat ; 33(6): 975-976, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32533563

RESUMO

Severe acute respiratory syndrome (SARS CoV-2/COVID-19) is a highly contagious and deadly disease caused by a virus belonging to the coronaviridae family. Researchers working in histopathology laboratories, dealing with morbid samples, are particularly vulnerable to infection unless they have very strong immunity. Hence, a proper precautionary protocol is required for the safety of the laboratory staff. The current review highlights the biological and physical agents that can be used to inactivate the virus and disinfect the surrounding environment in the laboratory.


Assuntos
Betacoronavirus/efeitos dos fármacos , Infecções por Coronavirus/prevenção & controle , Desinfetantes/farmacologia , Desinfecção/métodos , Histologia , Laboratórios/normas , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Infecções por Coronavirus/epidemiologia , Humanos , Controle de Infecções/métodos , Pessoal de Laboratório Médico , Serviço Hospitalar de Patologia/organização & administração , Serviço Hospitalar de Patologia/normas , Pneumonia Viral/epidemiologia
2.
Int. j. morphol ; 38(3): 634-639, June 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1098299

RESUMO

Dental education is in a continuous state of development to adapt to the changing learning strategies of the millennial students. The introduction of electronic learning resources (ELR) is a good example, which has added immense value to the traditional methods of teaching oral histology. The main purpose of the study was to analyze the dental students' perception of learning oral histology the way it was taught. A pre-approved questionnaire survey was electronically distributed to 129 students who had completed the oral histology course. The majority of the students viewed oral histology course to be difficult and irrelevant to their dental career. Similarly, most of them preferred studying alone and used ELR in place of books and atlases. More than three quarters believed using a microscope in practical training sessions will make the oral histology course easier and more interesting. The results of this study will be used to formulate recommendations to be implemented in oral histology course.


La educación dental se encuentra en un estado de desarrollo continuo para adaptarse a las cambiantes estrategias de aprendizaje de los estudiantes milenios. La introducción de recursos de aprendizaje electrónico (RAE) es un buen ejemplo, el cual ha agregado un valor importante a los métodos tradicionales de enseñanza de histología oral. El objetivo principal del estudio fue analizar la percepción de los estudiantes de odontología de aprender histología oral de la forma en que se enseñó. Una encuesta de cuestionario preaprobada se distribuyó electrónicamente a 129 estudiantes que habían completado el curso de histología oral. La mayoría de los estudiantes consideraron que el curso de histología oral era difícil e irrelevante para su carrera dental. Del mismo modo, la mayoría de ellos preferían estudiar solos y usaban RAE en lugar de libros y atlas. Más de las tres cuartas partes creen que usar un microscopio en sesiones de capacitación práctica, permitiría que el curso de histología oral sea más fácil e interesante. Los resultados de este estudio se utilizarán para formular recomendaciones que se implementarán en el curso de histología oral.


Assuntos
Humanos , Estudantes de Odontologia/psicologia , Educação em Odontologia/métodos , Boca/anatomia & histologia , Inquéritos e Questionários , Avaliação Educacional , Histologia/educação
3.
Sci Data ; 7(1): 165, 2020 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-32471976

RESUMO

The North Sea plaice, Pleuronectes platessa (Linnaeus, 1758), is a commonly studied commercial flatfish with poorly known ovarian histology. The following dataset is a collection of female plaice gonad images and their corresponding histological slides, collected during a complete season of the plaice's reproduction cycle. Stereology was used to determine the percentage of different structures found throughout the ovaries. Inter-agent calibrations were accomplished in order to harmonize the stereological readings, and were based on a comprehensive reading protocol and histological lexicon that were specifically written for the plaice's ovaries. The distribution and homogeneity of the different cell types found throughout the ovaries were also evaluated. This dataset can be used to automate the stereological reading process (through statistical learning methods for example) or to objectively determine the plaice's maturity phase, and link that information to either macroscopic measurements or through image analysis of the full ovaries.


Assuntos
Linguado , Imageamento Tridimensional , Ovário/anatomia & histologia , Animais , Feminino , Histologia , Ovário/citologia
4.
Rev. argent. mastología ; 39(141): 15-33, mar. 2020. graf
Artigo em Espanhol | LILACS | ID: biblio-1104364

RESUMO

Objetivos El objetivo principal del siguiente trabajo es relacionar los hallazgos imagenológicos con los resultados histopatológicos de lesiones mamarias que requirieron biopsia. El primer objetivo secundario es evaluar el grado de subestimación de aquellas lesiones de riesgo a las que se le realizó Biopsia Radioquirúrgica (brq). El segundo objetivo secundario es conocer la sensibilidad y especificidad del bi-rads como método de diagnóstico para nuestra muestra. Material y método Se incluyeron 403 pacientes que fueron biopsiadas en el Centro de Diagnóstico y Tratamiento de Enfermedades Mamarias (cedytem) de la Ciudad de Santa Fe, en el período de tiempo comprendido entre el 1/1/15 y el 31/12/17. Los nódulos fueron punzados bajo guía ecográfica y las microcalcificaciones, asimetrías y distorsiones arquitecturales bajo guía estereotáxica. La concordancia entre los hallazgos imagenológicos e histopatológicos fue analizada teniendo en cuenta la clasificación de Parikh y Tickman. Resultados Se constató una buena relación entre las categorías bi-rads y la probabilidad de malignidad de las lesiones acorde a lo establecido por el Colegio Americano de Radiólogos (acr). Se observó un 100% de concordancia entre las lesiones categorizadas como benignas (bi-rads 3) y un 97% entre las categorizadas como malignas (bi-rads 5). Las lesiones categorizadas en el grupo de moderada a alta sospecha de malignidad (bi-rads 4B y bi-rads 4C) que resultaron benignas fueron reevaluadas para decidir nuevo control en 6 meses, repetir la biopsia o indicar la realización de brq. Se diagnosticaron 17 lesiones de riesgo (4,2%), de las cuales 12 fueron reevaluadas con brq, evidenciando un porcentaje de subestimación del 42%. Conclusiones A partir de los datos obtenidos, se calculó una sensibilidad del bi-rads como método de diagnóstico del 95% y una especificidad del 74%


Objectives The primary aim of the following work is to relate the imaging findings to the histopathological results of breast lesions that required biopsy. The second aim is to evaluate the degree of underestimation of those risk lesions that underwent a radio-surgical biopsy. The last objective is to know the sensibility and specificity of bi-rads as a diagnosis method for our sample. Materials and method This work included four hundred and three patients who were biopsied at Center for the Diagnosis and Treatment of Breast Diseases (cedytem) in the city of Santa Fe, in the time period between 1/1/15 and 12/31/17. The nodules were punctured under ultrasound guidance while microcalcifications, asymmetries and architectural distortions under stereotactic guidance. Results A good relationship was observed between the bi-rads categories and the probability of malignancy of the lesions according to what is established by the American College of Radiologists (acr). The imaging-histopathological findings correlation was evaluated taking into account the classification of Parikh and Tickman. A 100% correlation was observed between lesions categorized as benign (bi-rads 3), and 97% among those categorized as malignant (bi-rads 5). The lesions categorized as moderate or high suspicion of malignancy (birads 4B and bi-rads 4C) which turned out to be benign were re-evaluated to decide on having a new control in 6 months, repeating the biopsy or indicating the radio-surgical biopsy. Seventeen risk lesions (4.2%) were diagnosed of which twelve were reassessed with radio-surgical biopsy, evidencing an underestimation percentage of 42%. Conclusions Based on the data obtained, it was calculated for our sample a 95% sensitivity of the bi-rads and a 74% of specificity as a diagnostic method.


Assuntos
Biópsia , Mama , Doenças Mamárias , Histologia
5.
Int. j. morphol ; 38(1): 38-42, Feb. 2020. tab
Artigo em Inglês | LILACS | ID: biblio-1056394

RESUMO

Students' perceptions and feedback have a significant impact on academic progress. The aim of this study was to determine the perceptions of medical students regarding the cumulative effects of the first year general histology course and the sophomore pathology introductory course, in addition to their perceptions regarding the curricular integration of histology and pathology. In this cross-sectional study, a questionnaire was given to second-year and third-year medical students in the middle of second semester. The questionnaire comprised several items on students' attitudes toward anatomic pathology, their feedback on the first year general histology and the sophomore pathology courses, and their perceptions regarding the integration of histology and pathology courses. A five-point Likert scale was used. Data were analyzed using Statistical Package for the Social Science (SPSS) v 20 software. Two hundreds and fourteen of the 236 questionnaires distributed were analyzed (response rate = 90.7 %). More than 51 % of the respondents reported that they couldn't identify the normal tissue counterpart of most practical pathology cases. Only 31.3 % thought their practical histology knowledge was beneficial for them in practical pathology. More than 87 % agreed or strongly agreed that pathology cases need to be copresented with normal tissue examples. A significant proportion of the respondents (60.7 %) were with merging histology and pathology in integrated courses. Pathology was of career choices for only 15.4 % of the participants. The curricular integration of histology and pathology in the first year needs to be tested, and much effort is needed to increase students' affinity for anatomic pathology.


Las percepciones y comentarios de los estudiantes tienen un impacto significativo en el progreso académico. El objetivo de este estudio fue determinar las percepciones de los estudiantes de medicina con respecto a los efectos acumulativos del curso de Histología general de primer año y del curso introductorio de Patología de segundo año, además de sus percepciones con respecto a la integración curricular de Histología y Patología. En este estudio transversal, se entregó un cuestionario a estudiantes de medicina de segundo y tercer año, a mediados del segundo semestre. Asimismo, el cuestionario comprendió varios aspectos referente a la actitud de los estudiantes hacia Patología Anatómica, sus comentarios sobre la Histología general en el primer año y los cursos de Patología de segundo año. Además se incorporaron las percepciones de los estudiantes con respecto a la integración de los cursos de Histología y Patología. Se utilizó una escala de Likert de cinco puntos. Los datos se analizaron utilizando el paquete Statistical Package for Social Science (SPSS) v 20. Se analizaron 214 de los 236 cuestionarios distribuidos (tasa de respuesta = 90,7 %). Más del 51 % de los encuestados indicaron estar de acuerdo o totalmente de acuerdo, en que no lograron identificar el tejido normal, en la mayoría de los casos de Patología práctica. Solo el 31,3 % observó que su conocimiento de Histología práctica era beneficioso para ellos durante la Patología práctica. Más del 87 % estuvo de acuerdo o muy de acuerdo en que los casos de Patología deben ser analizados con muestras de tejido normal. Un grupo importante de los encuestados (60,7 %) consideraba incorporar la Histología y la Patología en cursos integrados. Patología fue de elección en la carrera para el 15,4 % de los participantes. La integración curricular de Histología y Patología en el primer año, debe ser evaluada con el propósito de incrementar la afinidad de los estudiantes con la Patología Anatómica.


Assuntos
Humanos , Masculino , Feminino , Adulto , Adulto Jovem , Patologia/educação , Estudantes de Medicina/psicologia , Currículo , Histologia/educação , Percepção , Estudos Transversais , Inquéritos e Questionários , Avaliação Educacional
6.
Int. j. morphol ; 38(1): 56-60, Feb. 2020. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1056397

RESUMO

La lectura comprensiva de los términos médicos es relevante para un proceso de aprendizaje exitoso. Una de las estrategias para lograrlo es el conocimiento y aprendizaje de los orígenes latín y griego de las palabras, así el objetivo del presente trabajo fue investigar el significado y evolución del lexema griego plasma πλασµα y analizar la Terminologia Histologica en relación a un grupo de términos histológicos derivados de este lexema.


Comprehensive reading of medical terms is relevant to a successful learning process. One of the strategies to achieve this is the knowledge and learning of the Latin and Greek root of words. The aim of the present work was to investigate the meaning and evolution of the Greek lexeme plasma πλασµα, and to analyze the Terminologia Histologica in relation to a group of histological terms derived from this lexeme.


Assuntos
Humanos , Plasma , Histologia , Terminologia como Assunto
7.
J Agric Food Chem ; 68(10): 2880-2890, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-31603670

RESUMO

As a result of the high variability of fruit properties in the European plum Prunus domestica, a histochemical analysis of fruits at different stages of development was performed to understand the ripening process in cv. 'Colora' (yellow-red skinned) and cv. 'Topfive' (purple skinned). Histological analysis showed that carotenoids in the fruit had two different origins. In the fruit flesh, they derived from chloroplasts that turned into chromoplasts, whereas carotenoids in the fruit skin derived probably from proplastids. Flavan-3-ols and proanthocyanidins showed differential localization during ripening. They were visible in the vacuole in different fruit tissues or organized in tannosomes in the fruit flesh. Tanninoplasts were observed only in hypodermal cells of 'Colora'. Toward maturity, anthocyanins were detected in the epidermis and later in the hypodermis of both cultivars. The study forms a basis for the analysis of the biosynthesis of secondary metabolites in European plums and their biological effects.


Assuntos
Antocianinas/análise , Flavonoides/análise , Frutas/química , Proantocianidinas/análise , Prunus domestica/crescimento & desenvolvimento , Cloroplastos/química , Frutas/crescimento & desenvolvimento , Histologia , Plastídeos/química , Prunus domestica/química , Coloração e Rotulagem , Vacúolos/química
8.
Neotrop. ichthyol ; 18(1): e190092, 2020. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1098404

RESUMO

Cardinal tetra Paracheirodon axelrodi and bloodfin tetra Aphyocharax anisitsi are two species of characids with high trade value as ornamental fish in South America. Although both species inhabit middle water layers, cardinal neon exhibits a tropical distribution and bloodfin tetra a subtropical one. Generally, these species are difficult to grow, so it becomes essential to know some key components of the neuroendocrine system to achieve their reproduction in captivity. Considering the importance of deepening the knowledge of the reproductive physiology through functional morphology, for the first time in this work we performed an anatomical, morphological and immunohistochemical analysis of the pituitary gland of these two species. In both species, a leptobasic type pituitary is found in the ventral zone of the hypothalamus and it is characterized by a neurohypophysis which has a well-developed pituitary stalk and a globular adenohypophysis. The pituitary components, characterized by histochemistry and immunohistochemistry, shows a distribution pattern of cells types similar to other teleost species, with only slight differences in the distribution of βFSH and βLH for P. axelrodi.(AU)


El cardenal tetra Paracheirodon axelrodi y el tetra Aphyocharax anisitsi son dos especies de carácidos con alto valor comercial como peces ornamentales en América del Sur. Aunque ambas especies habitan en las capas medias de agua, el neón cardenal exhibe una distribución tropical, mientras que el tetra cola roja una distribución subtropical. En general estas especies son difíciles de cultivar, por lo que es esencial conocer algunos componentes clave de los sistemas neuroendocrinos para lograr su reproducción en cautiverio. Considerando la importancia de profundizar en el conocimiento de la fisiología reproductiva a través de la morfología funcional, en este trabajo realizamos, por primera vez, un análisis anatómico, morfológico e inmunohistoquímico de la glándula pituitaria de estas dos especies. En ambas especies, la hipófisis, del tipo leptobásica, se encontró en la zona ventral del hipotálamo y se caracteriza por una neurohipófisis con un tallo hipofisario bien desarrollado y una adenohipófisis globular. Los componentes hipofisarios, caracterizados por la histoquímica y la inmunohistoquímica, mostraron un patrón de distribución de tipos de células similares a otras especies de teleósteos, con solo pequeñas diferencias en la distribución de βFSH y βLH para P. axelrodi.(AU)


Assuntos
Animais , Hipófise/enzimologia , Imuno-Histoquímica/veterinária , Caraciformes/anatomia & histologia , Caraciformes/imunologia , Histologia
10.
Int. j. morphol ; 37(4): 1469-1474, Dec. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1040155

RESUMO

Histology belongs to the discipline of medical morphology. The knowledge is scattered and abstract in this discipline. It is difficult to the medical students beginner. Leading to poor effect on histology teaching. This study aimed to introduce medical students to the histology using barrier-based learning (BBL) method or traditional teaching method. We recruited 4 clinical medical classes, including two 5-years classes and two 7-years clinical medical classes, each of these classes randomly assigned to 1 of 2 groups. The control group received an introductory traditional teaching mode in histology. The experiment group received BBL method. Using final exam average scores, pass rate, excellent rates and phase tests to evaluate the teaching effect of these two teaching method. BBL teaching method is more effective than traditional teaching method, The application of BBL in histology is more easier to learning for the beginners of medical students.


La histología es una disciplina de la morfología médica. El conocimiento es disperso y abstracto en esta disciplina. Es una asignatura que resulta difícil para los estudiantes principiantes de medicina. Este estudio tuvo como objetivo presentar a los estudiantes de medicina la histología mediante el método de aprendizaje basado en barreras (BBL), comparándolo al método de enseñanza tradicional. Reclutamos estudiantes de 4 clases de medicina clínica, incluidas dos clases de quinto año y dos clases de medicina clínica de séptimo año, cada una de estas clases asignadas al azar a los grupos. El grupo de control recibió un método de enseñanza tradicional de introducción en histología. El grupo experimental recibió el método BBL. Usando los puntajes promedio del examen final, la tasa de aprobación, las tasas de excelencia y las pruebas de fase para evaluar el efecto de enseñanza de estos dos métodos de enseñanza, se determinó que el método de enseñanza de BBL es más efectivo que el método de enseñanza tradicional. La aplicación de BBL en histología permite un aprendizaje más sencillo para los estudiantes principiantes de medicina.


Assuntos
Aprendizagem Baseada em Problemas/métodos , Educação Médica/métodos , Histologia/educação , China
11.
Rev. bras. ortop ; 54(6): 692-696, Nov.-Dec. 2019. graf
Artigo em Inglês | LILACS | ID: biblio-1057962

RESUMO

Abstract Objective The present work aimed to evaluate the systemic effect of H. speciosa latex on bone neoformation. Methods For this, the latex was collected and diluted to 3% and 50%. A total of 28 Wistar rats were submitted to surgery to create a 5 mm diameter defect in the parietal bone. This experiment was conducted in 2 different periods: 1 and 2. For each period, the rats were divided into 3 groups: Control Group, Latex3 Group, and Latex50 Group, which received, respectively, daily administrations of 0.5 mL of distilled water, latex to 3% and latex to 50% by gavage, orally. The rats of periods 1 and 2 were euthanized, respectively, 15 and 30 days after the surgery, and the calvaria was collected. The results were analyzed using the ANOVA and Tukey tests; the significance level was 0.05. Results We show that, in each analyzed period, the experimental groups had the same amount of newly formed bone in the calvaria defect. Conclusion We conclude that daily and oral administrations of H. speciosa latex to 3% and to 50% over a period of 15 and 30 days does not contribute to the increase of the area of the newly formed bone in the calvaria defect.


Resumo Objetivo Este trabalho objetivou avaliar o efeito sistêmico do látex de H. speciosas obre a neoformação óssea. Métodos Para isso, o látex foi coletado e diluído a 3% e a 50%. Um total de 28 ratos Wistar foi submetido a cirurgia para a criação de um defeito de 5 mm de diâmetro no osso parietal. Esse experimento foi conduzido em dois períodos distintos: 1 e 2. Para cada período, os ratos foram divididos em 3 grupos: Grupo Controle, Grupo Látex3 e Grupo Látex50 que receberam, respectivamente, administrações diárias de 0,5 mL de água destilada, látex a 3% e látex a 50% por gavagem, via oral. Os ratos dos períodos 1 e 2 foram eutanasiados, respectivamente, 15 e 30 dias após a cirurgia e a calvária foi coletada. Os resultados foram analisados utilizando os testes ANOVA e Tukey; o nível de significância estabelecido foi 0,05. Resultados Mostramos que, em cada período analisado, os grupos experimentais tiveram a mesma quantidade de osso neoformado no defeito da calvária. Conclusão Portanto, concluímos que administrações diárias e orais do látex de H. speciosa a 3% e a 50% durante um período de 15 e 30 dias não contribui para o aumento da área do osso neoformado no defeito da calvária.


Assuntos
Animais , Ratos , Osso e Ossos , Terapias Complementares , Administração Oral , Apocynaceae , Hematoxilina , Histologia , Látex
12.
Educ. med. (Ed. impr.) ; 20(5): 280-283, sept.-oct. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-191830

RESUMO

INTRODUCCIÓN: La microscopia virtual se ha convertido en un recurso educativo alternativo para la enseñanza de la organización estructural de células, tejidos y órganos. Su uso mediante el acceso a páginas web de distintas instituciones actúa como refuerzo y sirve como material adicional combinado con el uso de la microscopia óptica convencional en las prácticas de la asignatura de Histología. OBJETIVO: Evaluar el interés de los alumnos de la asignatura Histología de Sistemas del 2.° curso del grado en Medicina por las imágenes virtuales procedentes de páginas web como complemento educativo. MATERIAL Y MÉTODO: El estudio se realizó sobre una muestra de 156 estudiantes del 2.° curso de Medicina mediante un acercamiento cuantitativo para realizar un análisis descriptivo. RESULTADOS: Los conceptos con las valoraciones más altas fueron para utilidad y mejora en comparación con los atlas convencionales. CONCLUSIONES: Los datos presentados en este estudio muestran una preferencia, todavía tímida, de estudio de la Histología mediante aprendizaje electrónico, aunque junto con los recursos tradicionales


INTRODUCTION: Virtual microscopy has become an educational resource alternative for teaching the structural organization of cells, tissues and organs. Its use through access to web pages of different institutions acts as reinforcement and additional material combined with the use of conventional optical microscopy in histology practices. OBJECTIVE: To evaluate the interest of the second-year medical students for virtual images from educational web pages as a complement during histology study. MATERIAL AND METHOD: The study was conducted on 156 second-year medical students through a quantitative approach to perform a descriptive analysis. RESULTS: Usefulness and improvement related to conventional atlas achieved the highest scores. CONCLUSIONS: The data presented in this study demonstrate a preference, still timid, for the study of histology through electronic learning nevertheless along with the traditional educational tools


Assuntos
Humanos , Alfabetização Digital , Histologia/educação , Aprendizagem , Educação Pré-Médica/métodos , Técnicas Histológicas/instrumentação , Estudantes Pré-Médicos/estatística & dados numéricos , Processamento de Imagem Assistida por Computador/métodos , Inquéritos e Questionários
13.
Rev. bras. ortop ; 54(5): 524-530, Sept.-Oct. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1057938

RESUMO

Abstract Objective Lung cancer is the leading cause of death by cancer, and the bones are one of the most common sites of metastasis from this condition. This study aimed to evaluate the influence of lung carcinoma histology on the frequency of bone metastases. Methods This retrospective study evaluated the medical records of 407 patients diagnosed with lung cancer between 2003 and 2012. The prevalence of bone metastases and their association with histological subtypes were evaluated using chi-squared tests, odds ratios (ORs) and 95% confidence intervals (CIs). The overall survival was evaluated using the Kaplan-Meier method. Results The prevalence of bone metastases was 28.2% (n = 115), and the spine was the most frequently affected site (98 metastases; 32.1%). Adenocarcinoma was the most common histological subtype of lung carcinoma (46.7%), and it was significantly more frequent among patients with bone metastases (58.3% versus 42.1%; p = 0.003; OR = 1.92; 95% CI: 1.29-2.97). Squamous cell carcinoma was significantly less frequent among patients with bone metastases (13.0% versus 29.8%; p = 0.0004; OR = 0.35; 95% CI: 0.19-0.64). The median survival time after the first bone metastasis diagnosis was 4 months. Conclusion Adenocarcinoma was the most common histological subtype of lung carcinoma, and it was significantly associated with a higher risk of developing bone metastases.


Resumo Objetivo O câncer de pulmão é a principal causa de morte por neoplasia, e os ossos são os principais locais de metástases desse tipo de câncer. O objetivo deste estudo foi avaliar a influência do tipo histológico do carcinoma de pulmão na frequência das metástases ósseas. Métodos Foram avaliados retrospectivamente os registros médicos de 407 pacientes diagnosticados com câncer de pulmão entre 2003 e 2012. A prevalência de metástases ósseas e suas associações com os subtipos histológicos foram avaliadas com o teste qui-quadrado, razão de probabilidade (RP), e intervalos de confiança (IC) de 95%. A sobrevida global foi avaliada com o método de Kaplan-Meier. Resultados A prevalência das metástases ósseas foi de 28,2% (n = 115), e a coluna vertebral foi o local mais frequente (98 metástases: 32,1%). O adenocarcinoma foi o subtipo histológico mais comum de carcinoma pulmonar (46,7%) e foi significativamente mais frequente entre os pacientes com metástases ósseas (13,0% versus 29,8%; p = 0,0004; OR = 0,35; 95% IC: 0,19-0,64). O tempo médio de sobrevida após o diagnóstico da primeira metástase óssea foi de 4 meses. Conclusão O adenocarcinoma foi o subtipo histológico mais comum de carcinoma pulmonar e foi significativamente associado a um maior risco de desenvolvimento de metástases ósseas.


Assuntos
Humanos , Masculino , Feminino , Osso e Ossos , Carcinoma de Células Escamosas , Adenocarcinoma , Estudos Retrospectivos , Morbidade , Causas de Morte , Histologia , Neoplasias Pulmonares , Metástase Neoplásica
14.
J Histotechnol ; 42(3): 150-162, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31492093

RESUMO

In clinical, research and veterinary laboratories of North America, large format histology has more recently been improved with newer equipment and better methodology. Large tissue specimens are frequently sliced in the grossing room and processed in multiple smaller, standard size tissue cassettes. Justifiably, submitting more blocks inherently lends itself to a greater confidence in the accuracy of the diagnosis, yet guidelines for tissue sampling often suggest taking fewer samples. For example, large tumor specimen protocols recommend taking one standard-sized tissue block for each cm diameter of tumor. However, cancers are the culmination of many complex changes in cell metabolism and often appear dissimilar at different tissue locations. As these changes have an uncertain behavior, many other tissue samples are often taken from areas that appear to have either a variable texture or color. Consequently, at microscopy, the complete tissue sample may need to be reassembled like a jigsaw puzzle as the stained sections are frequently presented over many slides. This problem has easily been overcome by using large format cassettes since the entire cross-section of the tissue sample can often be viewed on a single slide. Because these cassettes can effectively hold up to 10 times the volume of conventional standard size cassettes, they are a more efficient way of assessing large areas of tissue samples. This system is easily adapted for all tissue types and has become the established method for assessing large tissue samples in many laboratory settings.


Assuntos
Inclusão do Tecido/instrumentação , Inclusão do Tecido/métodos , Desenho de Equipamento , Histologia , Humanos , Laboratórios
15.
Balkan Med J ; 36(6): 347-353, 2019 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-31525846

RESUMO

Background: Predominant histologic subtypes have been reported as predictors of survival of patients with pulmonary adenocarcinoma. Aims: To evaluate the predictive value of histologic classification in resected lung adenocarcinoma using the classification systems proposed by the International Association for the Study of Lung Cancer, American Thoracic Society, European Respiratory Society, and World Health Organization (2015). Study Design: Cross-sectional study. Methods: The histologic classification of a large cohort of 491 patients with resected lung adenocarcinoma (stages I-III) was retrospectively analyzed. The tumors were classified according to their predominant component (lepidic, acinar, papillary, solid, micropapillary, and mucinous), and their predictive values were assessed for clinicopathologic characteristics and overall survival. Results: The patient cohort comprised 158 (32.2%) patients with solid predominant, 150 (30.5%) with acinar predominant, 80 (16.3%) with papillary predominant, 75 (15.3%) with lepidic predominant, 22 (4.5%) with mucinous, and 5 (1.0%) with micropapillary subtype, and 1 (0.2%) with adenocarcinoma in situ. Overall 5-year survival of 491 patients was found to be 51.8%. Patients with lepidic, acinar, and mucinous adenocarcinoma had 70.9%, 59.0%, and 66.6% 5-year survival, respectively, and there was no statistically significant difference between them. Whereas patients with solid, papillary, and micropapillary predominant adenocarcinoma had 41.0%, 40.5%, and 0.0% 5-year survival, respectively. Compared to other histologic subtypes, patients with solid and papillary predominant adenocarcinoma had significantly lower survival than those with lepidic (p<0.001, p=0.002), acinar (p<0.001, p=0.008), and mucinous (p=0.048, p=0.048) subtypes, respectively. The survival difference between patients with solid subtype and those with papillary subtype was not statistically significant (p=0.67). Conclusion: Solid and papillary histologic subtypes are poor prognostic factors in resected invasive lung adenocarcinoma.


Assuntos
Adenocarcinoma de Pulmão/diagnóstico , Adenocarcinoma de Pulmão/patologia , Histologia/classificação , Prognóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Estudos Retrospectivos
16.
APMIS ; 127(11): 727-730, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31418929

RESUMO

Aortic valve tissue excised during stenotic valve replacement surgery commonly exhibits histopathologic changes including prominent calcification of variable severity. We present briefly a case of a 78-year-old man with aortic valve stenosis and coronary artery disease undergoing aortic valve replacement and coronary artery bypass grafting. After pathologic examination of excised tissue, the aortic valve was determined to have nodular calcification and myxoid degeneration, as well as evidence of prominent, contiguous fatty infiltration of the valve's spongiosa layer. Although osseous and chondroid metaplasia have been described within excised cardiac valves, a significant constituent of adipose tissue contiguous through the length of a valve and not representing a discrete mass-forming, neoplastic lesion has been only described in isolated case reports.


Assuntos
Tecido Adiposo/patologia , Estenose da Valva Aórtica/cirurgia , Valva Aórtica/patologia , Idoso , Valva Aórtica/cirurgia , Estenose da Valva Aórtica/patologia , Ponte de Artéria Coronária , Próteses Valvulares Cardíacas , Histologia , Humanos , Masculino
17.
Clin Neuropathol ; 38(6): 285-293, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31296286

RESUMO

INTRODUCTION: Traditional teacher-centered histopathology training is based on theoretical lectures and practical tutorials. We hypothesize that learning outcomes improve if students are activated by demonstrating cardinal features of slides to each other and discussing their pathogenesis. Buzz groups (BGs) could facilitate this. The aim of this study was to investigate the effectiveness of student-centered BGs, i.e., peer-teaching, versus traditional teacher-centered histopathology teaching. Furthermore, we compared digital with analog microscopy. MATERIALS AND METHODS: In addition to traditional guided instruction and explanation of slides, neighboring students demonstrated to each other histopathological features and discussed associated pathogenesis. RESULTS: After only 4 course lessons, the BG students did much better than the control group (ANCOVA p = 0.002; F = 9.7). Then the control group also applied the BG technique. After another 4 lessons, the control group was able to catch up almost completely (ANCOVA p = 0.36; F = 0.9). Overall, there was no difference in time on task. DISCUSSION: Collaborative BGs improve the learning of histopathological competencies. They motivate and activate students to learn. The course also increased the appreciation of students for histopathology. For BGs, digital microscopy was better suited than traditional analog microscopy. The application of BGs in the context of analyzing microscopic images should be disseminated and studied on larger cohorts.


Assuntos
Educação de Graduação em Medicina/métodos , Histologia/educação , Patologia/educação , Humanos , Estudantes
19.
J Foot Ankle Surg ; 58(4): 713-716, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31256899

RESUMO

To compare the incidence of osteomyelitis based on different operational definitions using the gold standard of bone biopsy, we prospectively enrolled 35 consecutive patients who met the criteria of ≥21 years of age and a moderate or severe infection based on the Infectious Diseases Society of America classification. Bone samples were obtained from all patients by percutaneous bone biopsy or intraoperative culture if the patient required surgery. Bone samples were analyzed for conventional culture, histology, and 16S ribosomal RNA genetic sequencing. We evaluated 5 definitions for osteomyelitis: 1) traditional culture, 2) histology, 3) genetic sequencing, 4) traditional culture and histology, and 5) genetic sequencing and histology. There was variability in the incidence of osteomyelitis based on the diagnostic criteria. Traditional cultures identified more cases of osteomyelitis than histology (68.6% versus 45.7%, p = .06, odds ratio [OR] 2.59, 95% confidence interval [CI] 0.98 to 6.87), but the difference was not significant. In every case that histology reported osteomyelitis, bone culture was positive using traditional culture or genetic sequencing. The 16S ribosomal RNA testing identified significantly more cases of osteomyelitis compared with histology (82.9% versus 45.7%, p = .002, OR 5.74, 95% CI 1.91 to 17.28) and compared with traditional cultures but not significantly (82.9% versus 68.6%, p = .17, OR 2.22, 95% CI 0.71 to 6.87). When both histology and traditional culture (68.6%) or histology and genetic sequencing cultures (82.9%) were used to define osteomyelitis, the incidence of osteomyelitis did not change. There is variability in the incidence of osteomyelitis based on how the gold standard of bone biopsy is defined in diabetic foot infections.


Assuntos
Pé Diabético/complicações , Erros de Diagnóstico , Ossos do Pé/microbiologia , Ossos do Pé/patologia , Osteomielite/diagnóstico , Adulto , Biópsia , Técnicas de Cultura , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Diabetes Mellitus Tipo 2/complicações , Feminino , Histologia , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Osteomielite/etiologia , Osteomielite/microbiologia , Análise de Sequência de DNA
20.
Ann Anat ; 226: 16-22, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31330306

RESUMO

At first sight, the issue of morphological terminology may seem to be a "closed and unchanging chapter", as many of the structures within the human body have been known for decades or even centuries. However, the exact opposite is true. The initial knowledge of the microscopic structure of the human body has been continuously broadening thanks to the development of new specialized staining techniques, discovery of the electron microscope, or later application of histochemical and immunohistochemical methods into routine tissue examination. Contrary to popular belief, histology has a status of constantly developing scientific discipline, with continuous influx of new knowledge, resulting in an unavoidable necessity to revise the histological nomenclature at regular intervals. The team of experts of the Federative International Programme on Anatomical Terminology, a working group of the International Federation of Associations of Anatomists, published in 2008 the First Edition of Terminologia Histologica. Terminologia Histologica (TH) is the best and most extensive of all the histological nomenclatures ever issued. However, here we suggest that several terms of important histological structures are still missing while several other terms are disputable. First, we present some clinically important terms of cells and tissue structures for inclusion in the next TH and, in a second part, we refer to some new terms in the current edition of the TH which are not yet mentioned in current histology textbooks (e.g., fusocellular connective tissue, bundle bone as the third type of bone tissue, spongy layer of vagina or arteria vaginata from the splenic white pulp). With this article we hope to start a wide scientific discussion which will lead to an inambiguous definition and demonstration of typical examples of all terms in the TH, with the result that the new edition of the Terminologia Histologica will become an internationally accepted communication tool for all practitioners and teachers of histology alike.


Assuntos
Histologia/normas , Terminologia como Assunto , Anatomistas , Anatomia/normas , Células/classificação , Histologia/tendências , Humanos , Obras Médicas de Referência
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