Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 422
Filtrar
1.
Talanta ; 206: 120177, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514882

RESUMO

Two highly selective OFF-ON isomer fluorescent probes (1 and 2) for homo-/cysteine were designed and synthesized. The pyrene modified tetraphenylethylene derivative with AIE was used as luminescent group while maleimide was used as recognition group. These two isomer probes were found to be nearly nonfluorescent when treated with GSH. However, upon interaction with Cys or Hcy, the fluorescence was enhanced by 2000 folds in a wide pH range from 3 to 10. Experimental results and DFT calculation have demonstrated that the fluorescence OFF-ON switch of such thiol probes is resulted from the termination of the PET (photo-induced electron transfer) effect through the Michael addition reaction of maleimide unit and thiols. In addition, probe 1 and 2 exhibit excellent selectivity and sensitivity towards Cys, Hcy over GSH and other amino acids, which was confirmed by mass MS. We suggested that Michael addition reaction of these probes with GSH was prevented because of the stereo-hindrance effect. Furthermore, these two isomer probes were successfully used for imaging biothiols in living H1299 lung cancer cells.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Glutationa/química , Homocisteína/análise , Linhagem Celular Tumoral , Cisteína/química , Teoria da Densidade Funcional , Fluorescência , Corantes Fluorescentes/síntese química , Homocisteína/química , Humanos , Maleimidas/síntese química , Maleimidas/química , Microscopia de Fluorescência/métodos , Modelos Químicos , Estilbenos/síntese química , Estilbenos/química
2.
Medicine (Baltimore) ; 98(31): e16539, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31374015

RESUMO

BACKGROUND: Chronic stable angina (CSA) is a cardiovascular disease with high prevalence. At present, drug treatment is still the main measure of stable angina pectoris. Traditional Chinese medicine has a long history in the treatment of CSA. Qi stagnation and Blood stasis syndrome is a common syndrome of CSA. Xinnaoning (XNN) capsule is considered as an effective adjuvant treatment for CSA with the efficacy of promoting qi and blood circulation but lack of high-quality clinical evidence. The purpose of this study is to evaluate the efficacy and safety of XNN capsule compared with placebo by clinical trial. METHODS: This multicenter, randomized, double-blind, placebo-controlled trial will be conducted with a total of 240 participants diagnosed with chronic stable angina (qi stagnation and blood stasis syndrome). The participants will be randomized (1:1) into groups receiving either XNN or placebo for 12 weeks. After a 2-week run-in period, they will receive either XNN or placebo (3 pills, 3 times daily) for 12 weeks on the basis of conventional therapy. The primary outcomes include changes in the integral scores of angina symptoms. The secondary outcome measures include changes in the total score of traditional Chinese medicine syndrome, severity grading of angina pectoris, the number of angina pectoris per week, nitroglycerin dosage, score of seattle angina scale, serum homocysteine, incidence of cardiovascular events. Safety outcomes will also be assessed. Adverse events will be monitored throughout the trial. RESULTS: This study will investigate whether XNN capsule can alleviate clinical symptoms, and improve quality of life of patients with chronic stable angina (qi stagnation and blood stasis syndrome). The results of this study will provide clinical evidence for the application of XNN capsule in the treatment of chronic stable angina. TRIAL REGISTRATION: ClinicalTrials.gov: NCT03914131.


Assuntos
Angina Estável/terapia , Adulto , Idoso , Análise de Variância , Distribuição de Qui-Quadrado , Angiografia Coronária/métodos , Método Duplo-Cego , Feminino , Homocisteína/análise , Homocisteína/sangue , Humanos , Masculino , Medicina Tradicional Chinesa/métodos , Medicina Tradicional Chinesa/normas , Pessoa de Meia-Idade , Placebos , Inquéritos e Questionários , Resultado do Tratamento
3.
Molecules ; 24(17)2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31461829

RESUMO

In this work, a novel 7-hydroxybenzoxazinone-based fluorescent probe (PBD) for the selective sensing of biothiols is reported. Upon treatment with biothiols, PBD shows a strong fluorescence enhancement (up to 70-fold) and a large Stokes shift (155 nm). Meanwhile, this probe exhibits high resistance to interference from other amino acids and competing species. PBD features good linearity ranges with a low detection limit of 14.5 nM for glutathione (GSH), 17.5 nM for cysteine (Cys), and 80.0 nM for homocysteine (Hcy), respectively. Finally, the potential utility of this probe for biothiol sensing in living HeLa cells is demonstrated.


Assuntos
Benzoxazinas/química , Técnicas Biossensoriais/métodos , Corantes Fluorescentes/química , Cisteína/análise , Glutationa/análise , Células HeLa , Homocisteína/análise , Humanos , Limite de Detecção , Imagem Óptica
4.
Chem Commun (Camb) ; 55(63): 9311-9314, 2019 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-31310244

RESUMO

Discrimination between cysteine and homocysteine at the single-molecule level is achieved within a K238Q mutant aerolysin nanopore, which provides a confined space for high spatial resolution to identify the amino acid difference with a 5'-benzaldehyde poly(dA)4 probe. Our strategy allows potential detection and characterization of various amino acids and their modifications, and provides a crucial step towards developing nanopore protein sequencing devices.


Assuntos
Toxinas Bacterianas/química , Cisteína/análise , Homocisteína/análise , Nanoporos , Proteínas Citotóxicas Formadoras de Poros/química , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , Cromatografia Líquida de Alta Pressão , Mutagênese Sítio-Dirigida , Poli A/química , Proteínas Citotóxicas Formadoras de Poros/genética , Proteínas Citotóxicas Formadoras de Poros/metabolismo , Espectrometria de Massas por Ionização por Electrospray
5.
Analyst ; 144(17): 5075-5080, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31305811

RESUMO

Herein, we report the design of a novel fluorescent probe consisting of a naphthalimide fluorophore and a silicone small molecule for the reversible detection of hypochlorous acid and biothiol amino acids. The response mechanism of BSi-1 is based on the concept of the S-based oxidation/reduction. The probe was found to be suitable for imaging HOCl in HeLa, RAW 264.7 cells and zebrafish, demonstrating its utility in biological applications.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Ácido Hipocloroso/análise , Naftalimidas/química , Animais , Cisteína/química , Glutationa/química , Células HeLa , Homocisteína/química , Humanos , Limite de Detecção , Camundongos , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos , Estrutura Molecular , Oxirredução , Células RAW 264.7 , Peixe-Zebra
6.
Anal Chim Acta ; 1074: 123-130, 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31159932

RESUMO

Abnormal levels of Cys, Hcy and GSH are associated with various diseases, thus monitoring biothiols is of great significance. In this work, a dual-emission responsive near-infrared fluorescent probe NIR-NBD for detecting Hcy and Cys/GSH was developed based on the conjugation of a dicyanoisophorone based fluorophore (NIR-OH) and 7-nitrobenzofurazan (NBD). To our surprise, the addition of Hcy induced significant fluorescence enhancement at both 549 and 697 nm; while Cys/GSH resulted in major fluorescence emission at 697 nm. The detection limit was determined to be 33.2 nM for Cys, 33.5 nM for Hcy, and 34.4 nM for GSH. Therefore, the probe can be used for discriminative detection of Hcy and Cys/GSH. Moreover, fluorescence imaging of HeLa cells indicated that the probe was cell membrane permeable and could be used for visualizing Hcy and Cys/GSH in living cells.


Assuntos
4-Cloro-7-nitrobenzofurazano/análogos & derivados , Aminofenóis/química , Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , 4-Cloro-7-nitrobenzofurazano/síntese química , 4-Cloro-7-nitrobenzofurazano/toxicidade , Aminofenóis/síntese química , Aminofenóis/toxicidade , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/toxicidade , Células HeLa , Humanos , Limite de Detecção , Microscopia Confocal/métodos , Microscopia de Fluorescência/métodos
7.
Spectrochim Acta A Mol Biomol Spectrosc ; 222: 117262, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31212195

RESUMO

A fluorescent probe, 4-(benzothiazole-2-ylthio)-7-nitro-2,1,3-benzoxadiazole (TBT-NBD) was developed for cysteine (Cys) and homocysteine (Hcy). The reaction mechanism was based on the Cys/Hcy-induced nucleophilic substitution of benzothiazole thioether then Smiles rearrangement reaction to form corresponding amino-nitrobenzoxadiazole, which emitted yellow-green fluorescence and guaranteed the high selectivity for Cys/Hcy over glutathione (GSH). TBT-NBD could detect Cys/Hcy within 5 min in the presence of high concentration of GSH and other amino acids. Moreover, TBT-NBD had been exploited to identify intracellular Cys/Hcy in living cells in light of its low toxicity.


Assuntos
Benzotiazóis/química , Cisteína/análise , Corantes Fluorescentes/química , Homocisteína/análise , Sulfetos/química , Linhagem Celular Tumoral , Humanos , Microscopia de Fluorescência/métodos , Imagem Óptica/métodos , Espectrometria de Fluorescência/métodos
8.
Analyst ; 144(14): 4258-4265, 2019 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-31215916

RESUMO

Biothiols such as cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H2S) are widely found in mammalian cells. They are closely related to the production and metabolic pathways and play very important roles in physiological and pathological activities. Therefore, the quantitative detection of these biothiols is of great significance. Although many fluorescent probes have been successfully used to track biothiols in biological samples, the fluorescence method for simultaneously detecting these biothiols using separated fluorescence emission channels under single wavelength excitation is still immature. In this work, we prepared the conjugate of seminaphthorhodafluor (SNARF) dye and 7-nitro-1,2,3-benzoxadiazole (NBD) using as a simple long-wavelength fluorescent probe SNARF-NBD for specific detection of biothiols. Cys/Hcy and GSH/H2S were identified by two separated fluorescence emission channels under single wavelength excitation, which showed good selectivity and sensitivity. In addition, SNARF-NBD has low cytotoxicity and shows good imaging ability in living cells and zebrafish.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Sulfeto de Hidrogênio/análise , Animais , Corantes Fluorescentes/toxicidade , Camundongos , Imagem Óptica/métodos , Oxidiazóis/química , Oxidiazóis/toxicidade , Células RAW 264.7 , Rodaminas/química , Rodaminas/toxicidade , Espectrometria de Fluorescência/métodos , Peixe-Zebra
9.
Analyst ; 144(15): 4520-4525, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31241076

RESUMO

Using a simple fluorogenic reaction and the inner filter effect, a label-free ratiometric method for biothiol detection is developed. The reaction between dopamine and resorcinol can generate azamonardine with strong fluorescence. Since the absorption spectrum of azamonardine overlaps with the excitation spectrum of the mercaptopropionic acid-stabilized CdTe quantum dots (MPA-CdTe QDs), the emission of QDs can be quenched via the inner filter effect in the presence of azamonardine. Biothiols can inhibit the above fluorogenic reaction, resulting in the weakening of the fluorescence of azamonardine and the recovery of the emission of MPA-CdTe QDs. The fluorescence intensity ratio of azamonardine to MPA-CdTe QDs can be used to detect biothiols. This approach exhibited a linear trend to the biothiol concentrations in the range of 2 µM to 12 µM, with a limit of detection of 0.6 µM (S/N = 3). This ratiometric analysis strategy was further applied to detect biothiols in human serum samples, showing their great application potentials in real biological samples. The constructed method is simple, easy to prepare, and low-cost.


Assuntos
Cisteína/sangue , Corantes Fluorescentes/química , Pontos Quânticos/química , Espectrometria de Fluorescência/métodos , Compostos de Cádmio/química , Dopamina/química , Glutationa/análise , Homocisteína/análise , Humanos , Limite de Detecção , Resorcinóis/química , Telúrio/química
10.
Analyst ; 144(15): 4575-4581, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31233043

RESUMO

Although a large number of fluorescent probes have been developed, the simultaneous quantitative analysis of intracellular thiols is still difficult due to the spectral overlap and the complexity of the intracellular environment. In this study, a multi-signal fluorescent probe was employed for the simultaneous quantification of intracellular glutathione (GSH), cysteine (Cys) and homocysteine (Hcy). As the feature variables of the target components, the Tchebichef image moments (TMs) calculated from the grayscale images of the 3D fluorescence spectra were used to establish the quantitative linear models by stepwise regression. The intra-day and inter-day precisions of the proposed method were less than 5.6% and 8.7%, respectively. The recoveries ranged from 97.0% to 105.9%. In addition, the proposed approach was applied to the simultaneous quantitative determination of Cys, GSH and Hcy in the MCF-10A cell (a type of normal cell) and MDA-MB-231 cancer cell. The obtained results indicated that the concentrations of the three thiols in the cancer cell were higher than those in the normal cell. This study not only provides an effective approach for the quantification of multi-target bio-molecules in complicated intracellular environments, but also further extends the applications of multi-signal fluorescent probes, which will promote the design of new multi-signal fluorescent probes.


Assuntos
Benzotiazóis/química , Cumarínicos/química , Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Linhagem Celular Tumoral , Humanos , Análise dos Mínimos Quadrados , Limite de Detecção , Modelos Químicos , Análise de Componente Principal , Espectrometria de Fluorescência/métodos
11.
Analyst ; 144(11): 3676-3684, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31086902

RESUMO

Biothiols, including cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and H2S, play important roles in human physiological processes. However, it is a great difficulty to distinguish biothiols from each other because of their similar chemical properties. Based on Nile red, we have designed and synthesized a near-infrared fluorescent probe for discriminating Cys/Hcy from GSH/H2S by a dual-channel detection method. Using an ether bond, near-infrared Nile red was attached to 7-nitrobenzofurazan to construct the probe. Due to the photo-induced electron transfer, the probe showed almost no fluorescence from the green to red emission band. But upon the addition of Cys (0-150 µM) or Hcy (0-200 µM), the probe exhibited a noteworthy fluorescence "turn-on" signal in two unique emission bands (Green and Red) with a fast response (within 30 min). In contrast, the probe displayed an increase in fluorescence only in the red channel when encountering GSH (0-70 µM) or H2S (0-50 µM), and GSH/H2S could be tested respectively by different response time. The limit of detection was calculated to be 0.09 µM (Cys), 0.30 µM (Hcy), 0.24 µM (GSH), and 0.04 µM (H2S), respectively (based on S/N = 3). The desirable dual-channel detection could be achieved in serum samples and living cells. Moreover, the probe could be applied for bioimaging in mice, which indicated its potential application in the clinic.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Sulfeto de Hidrogênio/análise , 4-Cloro-7-nitrobenzofurazano/análogos & derivados , 4-Cloro-7-nitrobenzofurazano/síntese química , 4-Cloro-7-nitrobenzofurazano/toxicidade , Animais , Linhagem Celular Tumoral , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/toxicidade , Humanos , Concentração de Íons de Hidrogênio , Limite de Detecção , Camundongos Nus , Imagem Óptica/métodos , Oxazinas/síntese química , Oxazinas/química , Oxazinas/toxicidade , Espectrometria de Fluorescência
12.
Chem Asian J ; 14(13): 2220-2224, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31081238

RESUMO

Water-soluble ratiometric AIE-active fluorescent organic nanoparticles 2OA-FON for the specific sensing of cysteine over other biothiols are reported. The obtained amphiphilic probe included olefin aldehyde as recognizing unit, tetraphenylethylene as fluorescence reporter and lactose moiety as a hydrophilic group. This work provides a general design strategy based on the introduction of a sugar moiety into a hydrophobic AIEgen to develop ratiometric water-soluble fluorescent organic nanoparticles.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Nanopartículas/química , Estilbenos/química , Dimerização , Células Hep G2 , Humanos , Microscopia Confocal , Nanopartículas/ultraestrutura , Imagem Óptica , Solubilidade , Espectrometria de Fluorescência , Água/química
13.
Spectrochim Acta A Mol Biomol Spectrosc ; 218: 171-177, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-30991293

RESUMO

In this work, a novel probe D-HBT-NBD (1O) based on diarylethene to detect biothiols (including Cys, Hcy and GSH) was synthesized and the relative colorimetric and fluorescent properties were tested. The probe exhibited excellent photochromic properties and showed apparent colorimetric and fluorescent signals for Cys, Hcy and GSH. The probe can selectively detect Cys, Hcy and GSH by naked eyes for its open-ring isomer 1O and can discriminate Cys from Hcy/GSH by apparent color change from light orange to dark pink at the closed-ring state under the irradiation of UV light. At the excitation wavelength of 465 nm, the probe could be used to discriminate GSH from Cys/Hcy with no fluorescent emission at 570 nm. Taking advantage of the photochromic property of the diarylethene moiety and the different fluorescent properties of NBD derivatives of GSH and Cys/Hcy, 1O could be used as a novel probe to discriminate Cys, Hcy and GSH from each other simultaneously. Meanwhile, a logic gate was constructed based on the colorimetric and fluorescent properties of 1O.


Assuntos
Cisteína/análise , Etilenos/química , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Técnicas de Química Sintética , Colorimetria/métodos , Etilenos/síntese química , Corantes Fluorescentes/síntese química , Espectrometria de Fluorescência/métodos
14.
Electrophoresis ; 40(15): 1913-1920, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30892703

RESUMO

The enantiomeric separation of 9-fluorenylmethoxycarbonyl chloride (FMOC)-homocysteine (Hcy) by CE was investigated using γ-CD and the chiral ionic liquid (R)-(1-hydroxybutan-2-yl)(trimethyl)azanium-bis(trifluoromethanesulfon)imidate (also called (R)-N,N,N-trimethyl-2-aminobutanol-bis(trifluoromethane-sulfon)imidate) (EtCholNTf2 ) as chiral selectors. Using 2 mM γ-CD and 5 mM EtCholNTf2 in 50 mM borate buffer (pH 9), FMOC-Hcy enantiomers were separated with a resolution value of 3.8. A reversal in the enantiomer migration order in comparison with the single use of γ-CD in the separation buffer was obtained. Then, NMR experiments were carried out to elucidate the interactions taking place in the enantiomeric separation of FMOC-Hcy. NMR analyses highlighted the formation of an inclusion complex since the hydrophobic group of FMOC-Hcy was inserted into the γ-CD cavity. Moreover, interactions between EtCholNTf2 and γ-CD were also observed, suggesting that the chiral ionic liquid would also enter the cavity of the γ-CD.


Assuntos
Eletroforese Capilar/métodos , Homocisteína/isolamento & purificação , Líquidos Iônicos/química , Espectroscopia de Ressonância Magnética/métodos , gama-Ciclodextrinas/química , Homocisteína/análise , Homocisteína/química , Imidazóis/química , Estereoisomerismo
15.
Talanta ; 195: 197-203, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30625532

RESUMO

Biological thiols (biothiols), such as glutathione (GSH), cysteine (Cys) and homocysteine (Hcy), play a vital role in the process of reversible redox reactions in physiological systems. In this work, flow cytometry-based fluorescent sensor is for the first time developed for the detection of biothiols in a fluorescence "turn on" manner. The probe which we name "Polystyrene/Quantum Dots/Gold Nanoparticles" or (PS/QDs/Au) is constructed by immobilizing QDs onto the surface of PS microbeads to obtain fluorescent microbeads. The probe (PS/QDs/Au) is constructed by immobilizing QDs onto the surface of PS microbeads to obtain fluorescent microbeads, followed by gold NPs absorption through electrostatic interaction to quench their fluorescence. In the presence of biothiols, the fluorescence of our probe can be restored in less than 5 min, and the detection limits for GSH, Cys and Hcy are 0.5 µM, 0.1 µM and 0.3 µM, respectively. Most importantly, the fluorescence signal of each of our probe microbeads can be collected individually by flow cytometry, realizing single microbead-based biothiols detection for the first time. Moreover, the probe is successfully applied to imaging of intracellular biothiols in A549 cells, demonstrating its potential in biological application.


Assuntos
Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Células A549 , Sobrevivência Celular/efeitos dos fármacos , Cisteína/química , Citometria de Fluxo , Fluorescência , Corantes Fluorescentes/toxicidade , Glutationa/química , Ouro/química , Ouro/toxicidade , Homocisteína/química , Humanos , Nanopartículas Metálicas/química , Nanopartículas Metálicas/toxicidade , Microesferas , Poliestirenos/química , Poliestirenos/toxicidade , Pontos Quânticos/química , Pontos Quânticos/toxicidade
16.
Talanta ; 195: 281-289, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30625544

RESUMO

Biothiols, such as glutathione (GSH), homocysteine (Hcy) and cysteine (Cys), are important biomarkers and play crucial roles in many physiological processes. Thus, the detection of biothiols is highly important for early diagnosis of diseases and evaluation of disease progression. Herein, new types of BODIPY-based fluorescent probes (probe 1, probe 2 and probe 3) capable of cysteine (Cys)/homocysteine (Hcy) sensing with high selectivity over other amino acids were developed. In addition, we further studied the influence of different electronegativity substituents on these probes to sensing Cys/Hcy. Ultimately, we concluded that the electron withdrawing group on probe 1 can accelerate the probe response to Cys/Hcy, and probe 1 was successfully applied for selective imaging Cys/Hcy in living cells.


Assuntos
Compostos de Boro/química , Cisteína/análise , Corantes Fluorescentes/química , Homocisteína/análise , Cisteína/química , Células HeLa , Homocisteína/química , Humanos , Espectrometria de Fluorescência
17.
Med Sci Monit ; 25: 240-247, 2019 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-30617247

RESUMO

BACKGROUND This study observed the incidence of in-stent restenosis (ISR) after percutaneous coronary intervention (PCI) and discusses the risk factors of ISR based on clinical data, coronary angiography, and stent features, to provide a theoretical basis for the prevention and treatment of ISR. MATERIAL AND METHODS We selected 1132 cases who received stent implantation at the Shaanxi People's Hospital from June 2014 to June 2016 and were followed up by coronary angiography within 1 year. Based on coronary angiography, the cases were divided into ISR and non-ISR groups. ISR was defined as a reduction in lumen diameter by over 50% after PCI. The ISR group consisted of 93 cases and the non-ISR group consisted of 1039 cases. Medical history, biochemical indicators, features of coronary artery lesions, and stent status were analyzed retrospectively. Risk factors of ISR were identified by univariate and multivariate logistic regression analyses. RESULTS Among 1132 cases, 93 cases had ISR, with the overall incidence of 8.21%. Univariate and multivariate logistic regression analyses indicated that postoperative hypersensitive C-reactive protein (hs-CRP) levels (OR=2.309, 1.579-3.375 mg/L), postoperative homocysteine (HCY) levels (OR=2.202, 1.268-3.826 µmol/L), history of diabetes (OR=1.955,1.272-3.003), coronary bifurcation lesions (OR=3.785, 2.246-6.377), and stent length (OR=1.269, 1.179-1.365 mm) were independent risk factors of ISR after PCI (P<0.05). CONCLUSIONS Elevated hs-CRP and HCY levels after PCI, history of diabetes, coronary bifurcation lesions, and greater stent length were associated with a higher risk of ISR. Patients with a higher risk of ISR should receive routine follow-up and intense medication management after PCI to control the risk factors and to reduce ISR.


Assuntos
Reestenose Coronária/etiologia , Intervenção Coronária Percutânea/efeitos adversos , Stents/efeitos adversos , Idoso , Proteína C-Reativa/análise , China , Angiografia Coronária/efeitos adversos , Doença da Artéria Coronariana/complicações , Vasos Coronários/fisiopatologia , Feminino , Seguimentos , Homocisteína/análise , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco
18.
Talanta ; 196: 145-152, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30683344

RESUMO

A long-wavelength fluorescent probe NR-CY was developed for simultaneous identification of cysteine/glutathione and sulphide by combining the derivative of Nile red with 7-nitrobenzofurazan. The response of NR-CY to thiols is regulated by intramolecular charge transfer and photoinduced electron transfer mechanisms. For sulphide at 560 nm, cysteine at 475 nm and glutathione at 425 nm, different absorbance increases can be observed. NR-CY can detect cysteine at fluorescence emission 543 nm and distinguish sulphide from other analytes by kinetic experiments at 636 nm. The probe showed a rapid response to these thiols (cysteine was 90 s and sulphide was 30 s). In addition, NR-CY has been successfully applied to live MCF-7 cell imaging.


Assuntos
4-Cloro-7-nitrobenzofurazano/química , Cisteína/análise , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Oxazinas/química , Cisteína/química , Glutationa/química , Homocisteína/química , Humanos , Células MCF-7
19.
J Microbiol Biotechnol ; 29(1): 114-126, 2019 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-30518019

RESUMO

This paper introduces three ways to determine host-guest complexation of cucurbit[7]uril (CB[7]) with homocysteine (Hcy). After preincubating Hcy and cysteine (Cys) with CB[7], Ellman's reagent (DTNB) was used to detect Hcy and Cys. Only Cys reacted with DTNB and Hcy gave a retarded color change. This suggests that the -SH group of Hcy is buried inside CB[7]. Human cystathionine γ-lyase (hCGL) decreased the level of Hcy degradation after preincubating Hcy and CB[7]. These results suggest that the amount of free Hcy available was decreased by the formation of a Hcy-CB[7] complex. The immunological signal of anti-Hcy monoclonal antibody was decreased significantly by preincubating CB[7] with Hcy. The ELISA results also show that ethanethiol group (-CH2CH2SH) of Hcy, which is an epitope of anti-Hcy monoclonal antibody, was blocked by the cavity in CB[7]. Overall, CB[7] can act as a host by binding selectively with Hcy, but not Cys. The calculated half-complexation formation concentration of CB[7] was 58.2 nmol using Ellman's protocol, 97.9 nmol using hCGL assay and 87.7 nmol using monoclonal antibody. The differing binding abilities of Hcy and Cys towards the CB[7] host may offer a simple and useful method for determining the Hcy concentration in plasma or serum.


Assuntos
Bioensaio/métodos , Hidrocarbonetos Aromáticos com Pontes/química , Homocisteína/análise , Homocisteína/química , Imidazóis/química , Anticorpos Monoclonais/imunologia , Cistationina gama-Liase/química , Cisteína/química , Ácido Ditionitrobenzoico/química , Epitopos/imunologia , Homocisteína/imunologia , Humanos , Modelos Moleculares , Estrutura Molecular , Reagentes de Sulfidrila/química
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 209: 223-227, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30412847

RESUMO

Cysteine (Cys) is not only the central matter of sulfur metabolism in cells but also the only amino acid with reduced thiol group in 20 kinds of natural amino acids. In animal cells, Cys is taking part in many important and essential biological functions including protein synthesis, detoxification and metabolism. The development and application of fluorescent probes for the detection of Cys have attracted more and more attention and interest. Herein, we report a new fluorescent probe NFA that utilized naphthyl carboxy fluorescein as fluorophore and acryloyl group as reaction site for Cys specific detection. The probe essentially has weak fluorescence. Cys addition to NFA containing system induced distinct enhanced fluorescence emission which was attributed to the nucleophilic reaction of cysteine and acryloyl to release the fluorophore. The signal fluorescent response detection system allows NFA to be a reliable tool for Cys detection with low detection limit (0.58 µM). And NFA has been successfully applied for Cys imaging specifically in live Hela cells, which promotes the probe as a potential tool to understand the pathology of Cys related diseases.


Assuntos
Técnicas Biossensoriais/métodos , Cisteína/análise , Fluorescência , Corantes Fluorescentes/química , Glutationa/análise , Homocisteína/análise , Imagem Molecular/métodos , Células HeLa , Humanos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA