Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 5.611
Filtrar
2.
Theriogenology ; 141: 168-172, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31542520

RESUMO

This study evaluated embryo production after superovulation (SO) with a reduced number of FSH applications and increased eCG dose in 26 Bos taurus × Bos indicus donors. On Day 0, donors received an intravaginal device (CIDR) with 1.9 g of progesterone plus 2.5 mg of estradiol benzoate and 50 mg of progesterone via IM. On Day 4, donors were randomly allotted to one of three SO treatments: 1) 455 IU of Folltropin +400 IU of eCG (n = 9), 2) 350 IU of Folltropin +600 IU of eCG (n = 9), and 3) 500 IU of Pluset + 600 IU of eCG (n = 8). In treatment 455 IU of Folltropin +400 IU of eCG, donors received eight IM Folltropin injections in decreasing dose 12 h apart from Day 4 to Day 7. On Day 6, at the same time as the Folltropin, donors received via IM 25 mg of dinoprost tromethamine (PGF2a). On Day 7, the CIDR was removed, and together with the Folltropin, donors received 200 IU of eCG via IM. In treatment 350 IU of Folltropin +600 IU of eCG, donors received four IM Folltropin injections in decreasing dose 12 h apart on Days 4 and 5. On Day 6, donors received via IM 600 IU of eCG in the morning and two doses of 25 mg of PGF2a 12 h apart. On Day 7, the CIDR was removed. Donors from treatment 500 IU of Pluset +600 IU of eCG received four IM Pluset injections in decreasing dose 12 h apart on Days 4 and 5. On Day 6, donors received via IM 600 IU of eCG in the morning and two doses of 25 mg of PGF2a 12 h apart. On Day 7, the CIDR was removed. In the morning of Day 8, donors from the three treatments received 0.25 mg of GnRH via IM. Artificial insemination was performed on Day 8 (pm) and Day 9 (am). Embryos were collected on Day 15. Variables evaluated were number of CL before embryo collection, number of structures recovered, transferable embryos, degenerate embryos and unfertilized oocytes, recovery rate, and viability rate. There was no difference in any variable among treatments (P > 0.05). In conclusion, replacement of four Folltropin or Pluset injections from a conventional eight FSH-injection SO protocol, by a single injection of 600 IU of eCG, is a good alternative to reduce donor handling without decreasing yield of transferable embryos.


Assuntos
Bovinos/embriologia , Gonadotropina Coriônica/farmacologia , Hormônio Foliculoestimulante/farmacologia , Superovulação/efeitos dos fármacos , Animais , Gonadotropina Coriônica/administração & dosagem , Técnicas de Cultura Embrionária , Feminino , Fertilização In Vitro , Hormônio Foliculoestimulante/administração & dosagem , Doadores de Tecidos
3.
Theriogenology ; 142: 291-295, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31711702

RESUMO

The effectiveness of different treatments with recombinant equine FSH to stimulate follicular growth, multiple ovulations and embryo production in seasonally anovulatory mares was evaluated. During mid-winter season (July-August in Argentina, South America) forty light breed donor mares, presenting follicles <10 mm in diameter and no CL at ultrasound examination (deep-anestrus), were randomly assigned (n = 10/group) to one of the following treatments: Group 1: twice daily intramuscular (IM) injections of 0.65 mg reFSH (AspenBio Pharma, CO), Group 2: once daily IM injection of 1.3 mg reFSH, Group 3: twice daily IM injection of 0.32 mg reFSH, and Group 4: once daily IM injection of saline (control). Treatment was administered until a follicle of 35 mm was observed or for a total period of 10 days. When the largest follicle reached ≥35 mm in diameter, treatment was discontinued and 2500 IU hCG was injected intravenously (IV) 36 h later. Mares receiving hCG were inseminated with fresh semen every 48 h until ovulation(s) were detected or one dose of frozen semen (250 × 106 motile sperm) after the first ovulation was detected. Eight days after first ovulation, transcervical embryo recovery was performed. Recovered embryos were non-surgically transferred to anovulatory estrogen/progesterone treated recipients and pregnancy diagnosed by ultrasonography 7, 14 and 21 days later. All mares receiving reFSH, but none receiving saline control, responded to the treatment with follicular growth. On average, 6.5 days of reFSH treatment were required for mares to develop follicles of ovulatory size (>35 mm). Ovulations were detected in 80% of mares in Groups 1 and 2, 50% of mares in Group 3 and in none of Group 4 (Control). Among ovulating mares, no differences in number of ovulations, number of embryos recovered, or pregnancy rates were observed among reFSH treatments. Of treated mares, 6, 7, and 5 produced embryos in Groups 1, 2, and 3, respectively. The average embryo recovery rate per ovulated mare was 88%. The average embryo recovery rate per ovulation was 43%. Overall, a 59% pregnancy rate was achieved. These results indicate that treatment with reFSH during deep anestrus results in follicular development, ovulation of fertile oocytes, and production of embryos that established viable pregnancies after transfer. Also, a single daily administration of reFSH was as effective as two daily administrations, which allows for a simplified administration regimen.


Assuntos
Anovulação , Hormônio Foliculoestimulante/farmacologia , Recuperação de Oócitos , Indução da Ovulação/métodos , Taxa de Gravidez , Superovulação/efeitos dos fármacos , Doadores de Tecidos , Animais , Anovulação/tratamento farmacológico , Anovulação/patologia , Transferência Embrionária/métodos , Transferência Embrionária/veterinária , Feminino , Cavalos , Recuperação de Oócitos/estatística & dados numéricos , Recuperação de Oócitos/veterinária , Indução da Ovulação/veterinária , Gravidez , Proteínas Recombinantes/farmacologia , Estações do Ano
4.
Anim Reprod Sci ; 210: 106174, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31635775

RESUMO

Optimal results in cattle embryo transfer are limited by the variation in ova recovery, fertilization rate and embryo quality experienced with superovulation. Inflammation and immune dysregulation may be contributing factors. This study, evaluated feeding OmniGen-AF® (OG), a nutritional supplement that reduces inflammation and supports immune health, on superovulatory response and serum progesterone and cortisol concentrations in embryo donors treated with two different doses of Folltropin®-V (FSH). Angus cross-bred beef cows (n = 24) were assigned to four groups, fed OG at 0 or 56 g/animal/day for 49 days and were treated with 200 or 400 mg FSH to induce superovulation. Treatments for superovulation started after feeding OG for 28 days and ova were non-surgically recovered 7 days after estrus and graded for quality. More transferrable embryos (P < 0.05) were recovered from cows fed 56 g OG and treated with 400 compared with 200 mg FSH. Percent degenerate embryos recovered from cows treated with the 400 mg FSH dose was threefold greater (P < 0.05) when fed no OG compared with 56 g OG. Serum progesterone on day of embryo collection was greater (P < 0.05) in OG-supplemented cows and cows treated with 200 mg FSH. Serum cortisol was not affected (P > 0.10) by FSH dose or OG-feeding, but was greatest (P <  0.05) on Days 0 and 42 of the feeding period. In summary, the improvement in embryo quality with OG-feeding may relate to a greater serum progesterone concentration.


Assuntos
Bovinos/fisiologia , Suplementos Nutricionais , Hidrocortisona/sangue , Progesterona/sangue , Superovulação/efeitos dos fármacos , Doadores de Tecidos , Ração Animal , Animais , Dinoprosta/farmacologia , Técnicas de Cultura Embrionária/veterinária , Transferência Embrionária/veterinária , Sincronização do Estro , Feminino , Hormônio Foliculoestimulante/farmacologia , Inflamação/prevenção & controle , Inflamação/veterinária , Óvulo , Coleta de Tecidos e Órgãos
5.
Reprod Domest Anim ; 54 Suppl 4: 65-68, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31625245

RESUMO

The serine proteases, tissue- and urokinase-type plasminogen activators (PLAT and PLAU) and their inhibitors SERPINE1/2 are regulators of plasminogen to plasmin conversion. They are widely expressed in ovarian tissues, including granulosa and cumulus cells, and their expression is regulated by gonadotropins. The aim of this work was to assess the effect of serine protease inhibitors (aprotinin and AEBSF) and SERPINE1/2 on FSH-induced cumulus cell expansion, the production of prostaglandin E2 (PGE2) and retention of hyaluronic acid (HA) in expanding cumulus. The serine protease activity proved to be essential for the production of PGE2 and also for the retention of HA; the inhibition of plasminogen activators by SERPINE1/2 had the same effect. Collectively, these data indicate that plasmin is required for proper function of expanding cumulus cells in vitro and presumably also in vivo in the pre-ovulatory follicles.


Assuntos
Células do Cúmulo/efeitos dos fármacos , Dinoprostona/metabolismo , Oócitos/efeitos dos fármacos , Inibidor 1 de Ativador de Plasminogênio/farmacologia , Inibidores de Serino Proteinase/farmacologia , Serpina E2/farmacologia , Animais , Aprotinina/farmacologia , Células do Cúmulo/citologia , Células do Cúmulo/metabolismo , Feminino , Hormônio Foliculoestimulante/farmacologia , Ácido Hialurônico/metabolismo , Oócitos/citologia , Oócitos/metabolismo , Sulfonas/farmacologia , Suínos
6.
Zygote ; 27(5): 347-349, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31405397

RESUMO

The aim of this study was to investigate if there is an adverse effect of multiple controlled ovarian stimulation (COS) on the maturity of oocytes (MI and MII), fertilization rate, embryo developmental qualities and clinical pregnancy rates in donation cycles. In total, 65 patients undergoing oocyte donation cycles multiple times were included in this study. Patients were grouped as group A that consisted of donors with ≤2 stimulation cycles while B consisted of donors with ≥3 stimulation cycles; and group C included donors who had ≤15 oocytes, while group D had donors with ≥16 oocytes. Numbers of oocytes obtained, MI and MII oocytes, fertilization, embryo quality and clinical pregnancy outcomes were compared. Significant statistical differences were observed in total number of oocytes obtained, maturity of oocytes (MI and MII), fertilization rate, embryo qualities and clinical pregnancy outcomes of donors in groups A-D. Donors with ≤2 ovarian stimulation cycles had lower numbers of immature oocytes than donors with three or more stimulation cycles. However, donors with ≥3 stimulation cycles had higher numbers of mature oocytes, zygotes, with better day 3 embryo qualities and higher clinical pregnancy rates than donors with ≤2 stimulation cycles. Repeated COS does not seem to have any adverse effect on ovarian response to higher dose of artificial gonadotropin, as quality of oocytes collected and their embryological developmental potential were not affected by the number of successive stimulation cycles. The effect of multiple COS on the health of the oocyte donor needs to be assessed for future purpose.


Assuntos
Doação de Oócitos/métodos , Oócitos/fisiologia , Ovário/fisiologia , Indução da Ovulação/métodos , Doadores de Tecidos , Adolescente , Adulto , Blastocisto/citologia , Blastocisto/fisiologia , Implantação do Embrião , Feminino , Fertilização In Vitro , Hormônio Foliculoestimulante/farmacologia , Gonadotropinas/farmacologia , Humanos , Doação de Oócitos/efeitos adversos , Oócitos/citologia , Ovário/efeitos dos fármacos , Indução da Ovulação/efeitos adversos , Gravidez , Taxa de Gravidez , Adulto Jovem
7.
Reprod Domest Anim ; 54(11): 1443-1448, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31381183

RESUMO

To improve the efficiency of somatic cell nuclear transfer (SCNT) in sheep, we investigated the effects of recipient oocyte source, number of transferred embryos and season on the pregnancy and live lamb rates for sheep somatic cell nuclear transfer embryos. Follicle-stimulating hormone (FSH)-stimulated ovaries produced significantly more oocytes both in total and of suitable quality for maturation culture than those without FSH treatment (from slaughterhouse). However, their in vitro maturation rates were similar. Embryos were reconstructed using adult fibroblast cells into enucleated MII oocytes. The pregnancy and term rates were significantly higher in the FSH-stimulated group than in the slaughterhouse one. Oocytes from FSH-stimulated ovaries were enucleated as recipient cytoplasm for nuclear transfer in the following experiments. The transfer of 7-9 and 11-13 embryos produced significantly higher pregnancy rates than that of six embryos. However, the former groups exhibited similar live lamb rates. FSH-stimulated ovaries produced significantly more oocytes in November and December (winter) than in May to July (summer), but the associated maturation rate did not increase. Pregnancy and term rates were significantly higher when transfer occurred in winter than in summer. In conclusion, FSH treatment produced significant benefit regarding the number and quality of collected oocytes and also for the pregnancy and live lamb rates for reconstructed embryos. However, the transfer of an appropriate number of embryos (7-13) and at an appropriate season (winter) increased pregnancy and term rates.


Assuntos
Transferência Embrionária/veterinária , Técnicas de Transferência Nuclear/veterinária , Estações do Ano , Carneiro Doméstico/fisiologia , Animais , Feminino , Hormônio Foliculoestimulante/farmacologia , Técnicas de Maturação in Vitro de Oócitos/veterinária , Oócitos , Ovário/efeitos dos fármacos , Gravidez , Taxa de Gravidez
8.
Reprod Domest Anim ; 54(9): 1291-1303, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31339602

RESUMO

Adiponectin is an adipocyte-derived hormone regulating energy metabolism, insulin sensitivity and recently found to regulate reproduction. The current study was carried out to investigate gene and protein expression, immunolocalization of adiponectin and its receptors AdipoR1 and AdipoR2 in ovarian follicles of different developmental stages in water buffalo (Bubalus bubalis) and to investigate the effect of adiponectin on steroid production in cultured bubaline granulosa cells. qPCR, western blotting and immunohistochemistry were applied to demonstrate mRNA expression, protein expression and immunolocalization, respectively. The results indicate that adiponectin, AdipoR1 and AdipoR2 were present in granulosa cells (GC) and theca interna (TI) of ovarian follicles and the expression of adiponectin, AdipoR1, AdipoR2 in GC and AdipoR1 and AdipoR2 in TI increased with increase in follicle size (p < .05). Expression of adiponectin was high in small and medium size follicles in TI. The adiponectin and its receptors were immunolocalized in the cytoplasm of GC and TI cells. Further, in the in-vitro study, GCs were cultured and treated with recombinant adiponectin each at 0, 1 and 10 µg/ml alone or with follicle stimulating hormone (FSH) at 30 ng/ml) or Insulin-like growth factor I (IGF-I) at 10 ng/ml for 48 hr after obtaining 75%-80%s confluency. Adiponectin at 10 µg/ml increased IGF-I-induced estradiol (E2 ) and progesterone (P4 ) secretion and FSH-induced E2 secretion from GC and also increased the abundance of factors involved in E2 and P4 production (cytochrome P45019A1 [CYP19A1] and 3-beta-hydroxysteroid dehydrogenase [3ß-HSD]). In conclusion, this study provides novel evidence for the presence of adiponectin and its receptors in ovarian follicles and modulatory role of adiponectin on steroid production in buffalo.


Assuntos
Adiponectina/fisiologia , Búfalos/metabolismo , Receptores de Adiponectina/fisiologia , Adiponectina/genética , Adiponectina/farmacologia , Animais , Células Cultivadas , Estradiol/metabolismo , Feminino , Hormônio Foliculoestimulante/farmacologia , Regulação da Expressão Gênica , Células da Granulosa/metabolismo , Fator de Crescimento Insulin-Like I/farmacologia , Folículo Ovariano/metabolismo , Progesterona/metabolismo , Receptores de Adiponectina/genética , Células Tecais/metabolismo
9.
Eur J Pharmacol ; 860: 172560, 2019 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-31344364

RESUMO

Plants, fruits, and vegetables containing the bioflavonoid quercetin are widely used in food, beverages, and medicines; however, the effects of quercetin on reproductive processes and the possible mechanisms of quercetin action require extensive investigation. The aim of our study was to examine the direct effects of quercetin on basic ovarian cell functions and their response to follicle-stimulating hormone (FSH) and insulin-like growth factor I (IGF-I), known hormonal stimulators of reproduction. We analyzed the effects of quercetin alone (0, 1, 10, and 100 ng/ml) on cultured porcine ovarian granulosa cells or isolated ovarian follicles; or of quercetin (10 ng/ml) in combination with FSH (0, 0.01, 0.1, or 1 IU/ml) or IGF-I (0, 1, 10, or 100 ng/ml) on cultured porcine granulosa cells. The expression of proliferative (PCNA, cyclin B1) and apoptotic (BAX) markers, as well as markers for release of progesterone (P4), testosterone (T), and leptin (L), were measured by quantitative immunocytochemistry, Western immunoblotting, RT-qPCR, and EIA/RIA. Addition of quercetin reduced the accumulation of PCNA and cyclin B1, as well as their transcript levels, promoted the accumulation of BAX, decreased the release of P4 and L, and increased the release of T in cultured granulosa cells. In ovarian follicles, quercetin reduced the levels of both P4 and T. Exposure to FSH stimulated PCNA and decreased BAX accumulation, and increased the release of P4, T, and L. Quercetin inhibited and even reversed the effects of FSH. Like FSH, IGF-I also promoted granulosa cell proliferation and suppressed apoptosis. Quercetin did not modify IGF-I effects. These data suggest that the plant molecule quercetin can directly down-regulate basal ovarian cell functions (proliferation, apoptosis, and release of ovarian steroid and peptide hormones) and their response to the stimulatory activity of the upstream hormonal stimulator FSH.


Assuntos
Hormônio Foliculoestimulante/farmacologia , Células da Granulosa/citologia , Células da Granulosa/efeitos dos fármacos , Quercetina/farmacologia , Animais , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ciclina B1/metabolismo , Relação Dose-Resposta a Droga , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Células da Granulosa/metabolismo , Fator de Crescimento Insulin-Like I/farmacologia , Leptina/metabolismo , Progesterona/metabolismo , Antígeno Nuclear de Célula em Proliferação/genética , RNA Mensageiro/genética , Suínos , Testosterona/metabolismo
10.
Trop Anim Health Prod ; 51(8): 2641-2644, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31222711

RESUMO

The objective of the study was to evaluate embryo production in middle-aged and mature Bos taurus × Bos indicus cows induced to multiple ovulation (MO) in a tropical environment. Twenty-eight cows were assigned into two groups: (1) middle-aged cows = 4-6 years old (n = 13), and (2) mature cows = 8-12 years old (n = 15). All donors received the same MO protocol with follicle-stimulating hormone in decreasing dose during 4 days and two artificial insemination services. Total numbers of corpora lutea at embryo collection, structures collected, and viable embryos obtained, as well as recovery rate, were higher in middle-aged cows compared with mature cows (P < 0.05). A total number of degenerate embryos and unfertilized oocytes, as well as viability rate, were similar in both groups (P > 0.05). In conclusion, the mature cows responded to the MO treatment, but the average of viable embryos recovered per donor was lower than in middle-aged cows. Therefore, the inclusion of cows ≥ 8 years old as donors in MO programs in tropical environments should be avoided.


Assuntos
Bovinos/fisiologia , Embrião de Mamíferos/embriologia , Hormônio Foliculoestimulante/farmacologia , Inseminação Artificial/veterinária , Indução da Ovulação/veterinária , Ovulação/efeitos dos fármacos , Fatores Etários , Animais , Feminino , Hibridização Genética , México
11.
J Med Invest ; 66(1.2): 165-171, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31064932

RESUMO

As the follicular environment transits from being activin dominant to inhibin dominant during folliculogenesis, it is assumed that activin plays an important role in the early stage of follicular growth. We examined the effects of activin on morphological, biochemical and molecular changes in isolated preantral follicles. Preantral follicles were mechanically isolated from 14-day old female C57BL/6 mice. Each follicle was cultured and observed for 14 days usingan in vitro follicle culture system containing FSH, FSH + activin A and FSH + inhibin in the culture medium. We subsequently examined FSH receptor (FSH-R) mRNA expression in isolated follicle cultures with or without activin on days 0 and 2. Activin was observed to significantly stimulate follicle enlargement on days 2, 4, 6 and 8, accelerate morphological changes and increase estradiollevels in culture medium on days 4, 12 and 14. In contrast, inhibin did not alter follicular growth. Additionally, activin stimulated the expression of FSH-R mRNA in isolated granulosa cells. It was demonstrated that activin stimulated the growth of preantral follicles, mainly during the early stage of folliculogenesis, by inducing FSH-R expression, in an isolated follicle culture system. J. Med. Invest. 66 : 165-171, February, 2019.


Assuntos
Ativinas/farmacologia , Folículo Ovariano/efeitos dos fármacos , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Feminino , Hormônio Foliculoestimulante/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Folículo Ovariano/fisiologia , Receptores do FSH/análise , Receptores do FSH/genética
12.
Syst Biol Reprod Med ; 65(5): 350-356, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31099269

RESUMO

Information on the role of resistin on steroidogenesis is limited to animal studies. The aim of this study was to investigate the effect of various doses of resistin on estradiol and progesterone secretion from human luteinized granulosa cells in culture. Granulosa cells were obtained from follicular fluid aspirated from 50 women undergoing in vitro fertilization (IVF) treatment. The cells were cultured for 48 h after a 24 h pre-incubation period. The effect of resistin at dosages 1, 10 and 100 ng/ml alone or in combinations with FSH (10 and 100 ng/ml) on steroidogenesis was investigated. Estradiol and progesterone were measured by radioimmunoassays in culture supernatants at 24 h and 48 h. FSH treatment increased both estradiol and progesterone secretion. Resistin suppressed basal estradiol (at 1 ng/ml) and progesterone secretion (at all concentrations tested). When resistin (all concentrations) was combined with FSH (100 ng/ml), it eliminated the stimulatory effect of FSH on the secretion of estradiol and progesterone. This study indicates an inhibitory effect of resistin on the secretion of estradiol and progesterone by human luteinized granulosa cells in vitro. It is likely that this adipokine locally affects ovarian function in women. Abbreviations: 3ß-HSD: 3ß-hydroxysteroid dehydrogenase; CAP1: cyclase-associated protein 1; DCN: decorin; FIZZ: Found in Inflammatory Zones; hCG: human chorionic gonadotropin; IGF1: insulin-like growth factor type 1; IVF: in vitro fertilization; PCOS: polycystic ovary syndrome; RIA: radioimmunoassay; ROR1: receptor tyrosine kinase-like orphan receptor-1; TLR4: Toll-like receptor 4.


Assuntos
Estradiol/metabolismo , Células da Granulosa/efeitos dos fármacos , Células da Granulosa/metabolismo , Progesterona/metabolismo , Resistina/farmacologia , Adulto , Células Cultivadas , Relação Dose-Resposta a Droga , Feminino , Fertilização In Vitro , Hormônio Foliculoestimulante/farmacologia , Humanos
13.
Arch Gynecol Obstet ; 300(2): 441-446, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30976971

RESUMO

BACKGROUND: Although exogenous follicle-stimulating hormone (FSH) has been used for decades and millions of cycles have been performed worldwide until now, criteria for selecting the proper FSH starting dose have not been clearly identified. The aim of this study was to elaborate a formula based on markers of ovarian reserve for the calculation of the appropriate starting dose of FSH. METHODS: A total of 931 patients underwent in vitro fertilization (IVF) treatment using long GnRH agonist protocol was retrospectively identified and reviewed. 673 cases of them with a normal ovarian response (4-14 retrieved oocytes) were used to analysis the predictive formula. All follicles 4-7 mm in diameter were counted in the same day of blood sample in both ovaries using transvaginal ultrasound scan. The modified protocol of each patient was recorded and analyzed in the same center. In another center were the numbers of retrieved oocytes of 750 validated patients recorded and analyzed. RESULTS: A formula model based on age, AMH, and antral follicle count (AFC) was able to accurately predict the ovarian sensitivity and accounted for 57.2% of the variability of ovarian response to FSH. When tested in the same total population used to elaborate the model it predicts a high 46.88% rate of step-down protocol in higher-starting FSH dose group and about 57.92% of patients had their dose step-up modified in lower-starting FSH dose group during their treatment, respectively. And when tested in different population from another center used to elaborate the model it predicts a high 64.40% rate of ≥ 15 retrieved oocytes in higher-starting FSH dose group and about 22.50% of patients had ≤ 7 retrieved oocytes in lower-starting FSH dose group during their treatment, respectively. CONCLUSIONS: In the present study we demonstrated that the individualized FSH starting dose may be calculated on the basis of a woman's age, AMH and AFC. The formula model might be a useful, immediate, and easily applicable tool for clinicians to predict the tailored starting dose of FSH during their daily clinical practice.


Assuntos
Fertilização In Vitro/métodos , Hormônio Foliculoestimulante/uso terapêutico , Reserva Ovariana/fisiologia , Injeções de Esperma Intracitoplásmicas/métodos , Adulto , Feminino , Hormônio Foliculoestimulante/sangue , Hormônio Foliculoestimulante/farmacologia , Humanos , Estudos Retrospectivos , Resultado do Tratamento
14.
Environ Toxicol ; 34(7): 844-852, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30951242

RESUMO

Our goals were to investigate whether environmentally relevant doses of T-2 toxin can affect human ovarian granulosa cells' function and to reveal the potential mechanism of T-2 toxin's action. Results showed that T-2 toxin strongly attenuated luteinizing hormone/choriogonadotropin receptor (LHCGR) mRNA expression in follicle-stimulating hormone (FSH)-stimulated human cumulus granulosa cells. Addition of human chorionic gonadotropin was not able to elicit maximal response of ovulatory genes amphiregulin, epiregulin, and progesterone receptor. T-2 toxin reduced mRNA levels of CYP19A1 and steroidogenic acute regulatory protein (STAR) and lowered FSH-stimulated estradiol and progesterone production. Mechanistic experiments demonstrated that T-2 toxin decreased FSH-stimulated cyclic adenosine monophosphate (cAMP) production. Addition of total PDE inhibitor 3-isobutyl-1-methylxanthine prevented T-2 toxin's action on LHCGR, STAR, and CYP19A1 mRNA expression in FSH-stimulated human cumulus granulosa cells. Furthermore, T-2 toxin partially decreased 8-bromoadenosine 3'5'-cyclic monophosphate (8-Br-cAMP)-stimulated LHCGR and STAR, but did not affect 8-Br-cAMP-stimulated CYP19A1 mRNA expression in human cumulus granulosa cells. Overall, our data indicate that environmentally relevant dose of T-2 toxin decreases steroidogenesis and ovulatory potency in human cumulus granulosa cells probably through activation of PDE, thus posing a significant risk for female fertility.


Assuntos
Aromatase/genética , Células do Cúmulo/efeitos dos fármacos , AMP Cíclico/metabolismo , Hormônios Esteroides Gonadais/biossíntese , Fosfoproteínas/genética , Receptores do LH/genética , Toxina T-2/farmacologia , Adulto , Aromatase/metabolismo , Células Cultivadas , Gonadotropina Coriônica/metabolismo , Células do Cúmulo/metabolismo , Regulação para Baixo/efeitos dos fármacos , Regulação para Baixo/genética , Estradiol/metabolismo , Feminino , Hormônio Foliculoestimulante/metabolismo , Hormônio Foliculoestimulante/farmacologia , Células da Granulosa/efeitos dos fármacos , Células da Granulosa/metabolismo , Humanos , Fosfoproteínas/metabolismo , Progesterona/metabolismo , RNA Mensageiro/metabolismo , Receptores do LH/metabolismo , Adulto Jovem
15.
Endocrinology ; 160(7): 1561-1572, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-30942852

RESUMO

WNT signaling regulates a variety of ovarian processes, including follicle development, granulosa cell (GC) proliferation and differentiation, steroidogenesis, and ovulation. The secreted frizzled-related proteins (SFRPs) comprise a family of WNT signaling antagonists. Sfrp4 expression was previously reported to be induced in ovarian GCs and cumulus cells in vivo following human chorionic gonadotropin treatment, suggesting that it may play key roles in cumulus expansion, ovulation/luteinization, and corpus luteum (CL) function. In this study, we aimed to define the physiological roles of Sfrp4 in the ovary by gene targeting. Sfrp4-null female mice were found to produce larger litters than did their wild-type littermates. Although previous studies had suggested roles of Sfrp4 in luteal cell survival, no differences in CL formation, morphology, steroidogenesis, involution, or luteal cell apoptosis were found in Sfrp4-null mice. Likewise, cumulus expansion occurred normally in Sfrp4-null mice, with minimal changes in cumulus cell gene expression. Hyperfertility in the Sfrp4-null model was ultimately attributed to decreased antral follicle atresia, leading to an enhanced ovulatory rate. Increased expression of FSH- and LH-responsive genes was found in GCs from Sfrp4-null mice, and GCs isolated from Sfrp4-null mice were found to be hyperresponsive to FSH and LH in vitro. Although Sfrp2 was found to be overexpressed in the GCs of Sfrp4-null mice (suggesting a compensatory mechanism), Sfrp2-null mice had normal fertility and ovulatory rates, and Sfrp2/4 double knockout mice did not differ from Sfrp4-null mice. Taken together, our results suggest that SFRP4 acts to attenuate GC responsiveness to gonadotropins, thereby decreasing follicle survival, ovulatory rate, and fertility.


Assuntos
Fertilidade/fisiologia , Folículo Ovariano/metabolismo , Ovulação/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Animais , Apoptose/fisiologia , Corpo Lúteo/metabolismo , Células do Cúmulo/metabolismo , Feminino , Hormônio Foliculoestimulante/farmacologia , Células da Granulosa/efeitos dos fármacos , Células da Granulosa/metabolismo , Tamanho da Ninhada de Vivíparos , Hormônio Luteinizante/farmacologia , Camundongos , Camundongos Knockout , Folículo Ovariano/crescimento & desenvolvimento , Proteínas Proto-Oncogênicas/genética , Transdução de Sinais/fisiologia
16.
Theriogenology ; 132: 27-35, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30986612

RESUMO

Basic fibroblast growth factor (bFGF) and follicle-stimulating hormone (FSH) both play important roles in primordial follicle development. Here we investigated the reciprocal stimulation effects of a cytokine bFGF and FSH on primordial follicle development in the chicken and considered a possible signaling mechanism involving protein kinase B (AKT) and extracellular regulated protein kinase (ERK) pathways. 4-day-old chicken ovaries were treated with bFGF and FSH for 3 days in culture to investigate the effects of bFGF and FSH on primordial follicle development. Methods included HE staining, immunohistochemistry, quantitate real-time PCR, Western blot and immunofluorescence. A correlated change of bFGF receptor (FGFR1) mRNA expression and time course of primordial follicle activation was revealed in the early chick ovaries. A reciprocal stimulation effect on primordial follicle activation was demonstrated for bFGF and FSH, along with accelerated granulosa cells proliferation and decreased cell apoptosis. The promoting effect of bFGF was attenuated by the FGFR1 inhibitor SU5402 where the percentage of growing follicles had decreased. AKT and ERK signaling pathways mediated the action of bFGF and FSH in their promotion of primordial follicle activation. Cytokine bFGF and FSH imposed reciprocal stimulating effects on granulosa cell proliferation and anti-apoptosis to promote primordial follicle activation via the PI3K-AKT and ERK signaling pathways in early chick ovaries.


Assuntos
Galinhas , Fator 2 de Crescimento de Fibroblastos/farmacologia , Hormônio Foliculoestimulante/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Folículo Ovariano/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Feminino , Regulação da Expressão Gênica/fisiologia , Folículo Ovariano/fisiologia , Transdução de Sinais/efeitos dos fármacos
17.
Int J Mol Sci ; 20(5)2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30866587

RESUMO

The maturation of mammalian oocytes in vitro can be stimulated by gonadotropins (follicle-stimulating hormone, FSH) or their intrafollicular mediator, epidermal growth factor (EGF)-like peptide-amphiregulin (AREG). We have shown previously that in pig cumulus-oocyte complexes (COCs), FSH induces expression and the synthesis of AREG that binds to EGF receptor (EGFR) and activates the mitogen-activated protein kinase 3/1 (MAPK3/1) signaling pathway. However, in this study we found that FSH also caused a rapid activation of MAPK3/1 in the cumulus cells, which cannot be explained by the de novo synthesis of AREG. The rapid MAPK3/1 activation required EGFR tyrosine kinase (TK) activity, was sensitive to SRC proto-oncogene non-receptor tyrosine kinase (SRC)-family and protein kinase C (PKC) inhibitors, and was resistant to inhibitors of protein kinase A (PKA) and metalloproteinases. AREG also induced the rapid activation of MAPK3/1 in cumulus cells, but this activation was only dependent on the EGFR TK activity. We conclude that in cumulus cells, FSH induces a rapid activation of MAPK3/1 by the ligand-independent transactivation of EGFR, requiring SRC and PKC activities. This rapid activation of MAPK3/1 precedes the second mechanism participating in the generation and maintenance of active MAPK3/1-the ligand-dependent activation of EGFR depending on the synthesis of EGF-like peptides.


Assuntos
Anfirregulina/metabolismo , Células do Cúmulo/citologia , MAP Quinases Reguladas por Sinal Extracelular/genética , Hormônio Foliculoestimulante/farmacologia , Oócitos/citologia , Animais , Células Cultivadas , Células do Cúmulo/efeitos dos fármacos , Células do Cúmulo/metabolismo , Receptores ErbB/metabolismo , Feminino , Técnicas de Maturação in Vitro de Oócitos , Proteína Quinase 1 Ativada por Mitógeno/genética , Proteína Quinase 3 Ativada por Mitógeno/genética , Oócitos/efeitos dos fármacos , Oócitos/metabolismo , Transdução de Sinais/efeitos dos fármacos , Suínos , Ativação Transcricional , Quinases da Família src/metabolismo
18.
Behav Neurol ; 2019: 7694503, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30891100

RESUMO

Objective: To compare baseline and 72-hour hormone levels in women with traumatic brain injury (TBI) and controls. Setting: Hospital emergency department. Participants: 21 women ages 18-35 with TBI and 21 controls. Design: Repeated measures. Main Measures: Serum samples at baseline and 72 hours; immunoassays for estradiol (E2), progesterone (PRO), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and cortisol (CORT); and health history. Results: Women with TBI had lower E2 (p = 0.042) and higher CORT (p = 0.028) levels over time. Lower Glasgow Coma Scale (GSC) and OCs were associated with lower FSH (GCS p = 0.021; OCs p = 0.016) and higher CORT (GCS p = 0.001; OCs p = 0.008). Conclusion: Acute TBI may suppress E2 and increase CORT in young women. OCs appeared to independently affect CORT and FSH responses. Future work is needed with a larger sample to characterize TBI effects on women's endogenous hormone response to injury and OC use's effects on post-TBI stress response and gonadal function, as well as secondary injury.


Assuntos
Fatores Etários , Lesões Encefálicas Traumáticas/metabolismo , Lesões Encefálicas/metabolismo , Hormônio Foliculoestimulante/farmacologia , Hormônio Luteinizante/farmacologia , Adolescente , Adulto , Estradiol/metabolismo , Feminino , Hormônio Foliculoestimulante/metabolismo , Humanos , Hormônio Luteinizante/metabolismo , Progesterona/metabolismo , Adulto Jovem
19.
Theriogenology ; 129: 168-177, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30856402

RESUMO

Understanding regulators of folliculogenesis remains limited in the domestic dog (Canis familiaris), which challenges our ability to develop in vitro follicle culture systems for canid genome rescue efforts. Here, we investigated the influence of activin on dog follicle development and survival, oocyte quality, and FSH receptor expression in culture. Preantral (150 - ≤230 µm diameter), early antral (231 - ≤330 µm), and antral (>330-550 µm) stage follicles were encapsulated in a fibrin-alginate hydrogel with 0, 100, or 200 ng/ml rhActivin plus 0, 0.1, 1, or 10 µg/ml FSH for 12 or 21 d of in vitro culture. All follicle groups increased in diameter (P < 0.05) with activin acting synergistically with FSH to improve (P < 0.05) growth and antral cavity expansion (to >630 µm) in early antral and antral cohorts. This complementary effect was not linked to changes in FSHR mRNA expression (P > 0.05). Although not influencing (P > 0.05) follicle survival or transzonal projection (TZP) density in shorter term 12 d culture, activin in the presence of 1 ng/ml FSH maintained TZP density from the 12-21 d interval. Activin also increased oocyte diameter and improved nuclear integrity compared to un-supplemented controls. These results indicate that activin acts synergistically with FSH to promote growth and antral cavity expansion of the dog follicle in vitro, information useful to formulating an effective culture microenvironment for this species.


Assuntos
Ativinas/farmacologia , Cães/fisiologia , Folículo Ovariano/efeitos dos fármacos , Animais , Técnicas de Cultura de Células/veterinária , Feminino , Hormônio Foliculoestimulante/farmacologia , Oócitos/crescimento & desenvolvimento , Folículo Ovariano/crescimento & desenvolvimento , Receptores do FSH/metabolismo , Regulação para Cima
20.
Theriogenology ; 129: 61-69, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30822644

RESUMO

This study analyzed IGF-1 protein immunostaining in sheep ovaries, the effect of IGF-1 alone or associated with FSH on the culture of secondary follicles, and the immunostaining of LHR protein in antral follicles before and after culture. Ovaries were collected for IGF-1 protein analysis. In experiment 1, secondary follicles were cultured in α-MEM+ (control) or α-MEM+ supplemented with IGF-1 (10, 50 or 100 ng/mL). In experiment 2, follicles were cultured in the same media of experiment 1 plus 750 ng/mL FSH. Moreover, LHR immunostaining was analyzed in fresh antral follicles and after culture in 50 ng/mL IGF-1 + FSH. The IGF-1 protein was immunolocalized in oocytes from all stages of follicle development and in the granulosa cells from secondary and antral follicles. IGF-1 did not influence (P > 0.05) follicular viability and growth (experiment 1). However, in experiment 2, 50 ng/mL IGF-1 + FSH stimulated oocyte growth (P < 0.05) and LHR immunostaining in antral follicles. Control medium, 10 or 50 ng/mL IGF-1 + FSH showed similar levels of reactive oxygen species, glutathione and active mitochondria (P > 0.05). In conclusion, the IGF-1 protein is present in all ovarian follicle stages in sheep. Moreover, the association between 50 ng/mL IGF-1 and FSH has a synergistic effect in vitro, increasing the percentage of fully grown oocytes and the intensity of immunostaining of LHR protein in oocytes and granulosa cells of cultured antral follicles.


Assuntos
Hormônio Foliculoestimulante/farmacologia , Fator de Crescimento Insulin-Like I/análise , Folículo Ovariano/crescimento & desenvolvimento , Ovário/metabolismo , Receptores do LH/análise , Ovinos , Animais , Técnicas de Cultura de Células/veterinária , Feminino , Glutationa/metabolismo , Imuno-Histoquímica/veterinária , Técnicas de Maturação in Vitro de Oócitos/métodos , Técnicas de Maturação in Vitro de Oócitos/veterinária , Fator de Crescimento Insulin-Like I/farmacologia , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/metabolismo , Espécies Reativas de Oxigênio/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA