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1.
Medicine (Baltimore) ; 98(44): e17839, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31689874

RESUMO

INTRODUCTION: The incidence of malignant melanoma has been rising steadily over the past decades and Merkel cell carcinoma is a highly aggressive neuroendocrine skin tumor with a high mortality rate. Sentinel lymph node (SLN) biopsy is a recommended prognostic tool in primary cutaneous malignant melanomas of intermediate thickness and in all clinically node-negative Merkel cell carcinomas. The gold standard method for identification of SLNs is lymphoscintigraphy, which involves radioactive tracers. Indocyanine green-based near-infrared fluorescence imaging (NIRFI) has been also used for intraoperative SLN identification. We aim to evaluate the diagnostic sensitivity of the VisionSense VS3 NIRFI device for SNL identification. This device uses stereoscopic 3D high definition for both fluorescence and visible light imaging. Our hypothesis is that SLNs may be identified transcutaneously using fluorescent dye injections and NIRFI; therefore, obviating the need for lymphoscintigraphy in the future. METHODS AND ANALYSIS:: lymph node identification in skin malignancy using indocyanine green transcutaneously is a prospective diagnostic sensitivity study conducted at the Department of Plastic and Hand Surgery at the University Hospital Berne, Inselspital, Switzerland. The study aims at recruiting 93 patients (start date September 2017) to compare the accuracy of VisionSense VS3 camera at identifying SLNs transcutaneously with the current gold standard, lymphoscintigraphy. Moreover, a secondary objective is to determine if anatomical location of the SLN and patient factors (eg, body mass index, age) have an impact on the ability of VisionSense to detect SLNs when compared with the same gold standard. TRIAL REGISTRATION NUMBER: ClinicalTrials.gov NCT03545334.


Assuntos
Corantes Fluorescentes , Verde de Indocianina , Imagem Óptica/instrumentação , Imagem Óptica/métodos , Linfonodo Sentinela/diagnóstico por imagem , Neoplasias Cutâneas/diagnóstico por imagem , Administração Cutânea , Adulto , Carcinoma de Célula de Merkel/diagnóstico por imagem , Carcinoma de Célula de Merkel/patologia , Humanos , Melanoma/diagnóstico por imagem , Melanoma/patologia , Estudos Prospectivos , Método Simples-Cego , Neoplasias Cutâneas/patologia
2.
Inorg Chem ; 58(18): 12422-12432, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31483641

RESUMO

Fluorescence imaging is a powerful tool in biomedical research. It has been frequently used to uncover or better understand physiological mechanisms in disease-related processes such as cancer. The majority of chromophores used for imaging are based on a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) scaffold. However, their applications are limited due to their poor water solubility as well as poor cancer cell selectivity. To circumvent these drawbacks, we present herein the use of bis(dipyrrinato)zinc(II) complexes. As this class of compounds is associated with a quenching effect of the excited state in water, the lead compound of this study (3) was encapsulated in a polymer matrix with biotin as a targeting moiety (3-NP). This encapsulation improved the water solubility, overcame the quenching effects in water, as well as allowed selective accumulation in the lysosomes with a bright fluorescence signal in monolayer cells as well as 3D multicellular tumor spheroids (MCTS). As a benefit from the biotin targeting moiety, the nanoparticles were majorly taken up by the sodium dependent multivitamin transporter (SMVT) which is overexpressed in various cancers cells and selectively accumulated in cancerous cells over noncancerous cells.


Assuntos
Corantes Fluorescentes/química , Lisossomos/patologia , Nanopartículas/química , Neoplasias/patologia , Polímeros/química , Pirróis/química , Zinco/química , Células A549 , Linhagem Celular , Complexos de Coordenação/química , Células HeLa , Humanos , Lisossomos/ultraestrutura , Microscopia Confocal/métodos , Neoplasias/diagnóstico por imagem , Imagem Óptica/métodos
3.
Zhonghua Zhong Liu Za Zhi ; 41(9): 654-658, 2019 Sep 23.
Artigo em Chinês | MEDLINE | ID: mdl-31550854

RESUMO

Objective: This study aims to explore the clinical value of fusion indocyanine green fluorescence imaging (FIGFI) in total laparoscopic radical resection for right colon cancer. Methods: From October, 2018 to December, 2018, 15 patients who underwent total laparoscopic radical resection for right colon cancer using FIGFI in Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College were retrospectively enrolled in this study. Data regarding surgical outcomes, postoperative recovery, pathological outcomes and complications were collected and analyzed. Results: All patients successfully underwent total laparoscopic radical resection for right colon cancer using FIGFI. 1 patients (6.7%) received extended resection of bowel due to poor blood supply after mesentery excision. The average operation time was 133.7 minutes and intraoperative blood loss was 26.7 ml. The average time to ground activities, fluid diet intake, first flatus and postoperative hospitalization were 19.1 h, 11.7 h, 32.5 h and 5.0 d, respectively. The average length of tumor was 4.5 cm. The average proximal and distal resection margins were 14.9 cm and 12.1 cm, respectively. The average number of lymph nodes retrieved was 29.3 per patient. Only one patient suffered from incisional fat liquefaction after surgery and was managed effectively by regular dressing change. No severe complications such as indocyanine green allergy, anastomotic stenosis, anastomotic leakage, abdominal bleeding, bowel obstruction, pulmonary infection, and abdominal infection occurred in any patients. Conclusions: FIGFI is helpful to judge the blood supply of intestinal segments and anastomotic stoma in total laparoscopic radical resection for right colon cancer quickly. It is a safe and feasible technique with satisfactory short-term effect.


Assuntos
Anastomose Cirúrgica/métodos , Colectomia/métodos , Colo/irrigação sanguínea , Neoplasias do Colo/cirurgia , Corantes/administração & dosagem , Verde de Indocianina/administração & dosagem , Imagem Óptica/métodos , Fístula Anastomótica/prevenção & controle , Colo/cirurgia , Procedimentos Cirúrgicos do Sistema Digestório/métodos , Estudos de Viabilidade , Humanos , Laparoscopia/métodos , Estudos Retrospectivos , Resultado do Tratamento
4.
Medicine (Baltimore) ; 98(36): e16935, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31490376

RESUMO

BACKGROUND: Although several previous studies demonstrated the feasibility and efficacy of indocyanine green (ICG) for thyroid cancer surgery, ICG was administered through venous injection and focused on parathyroid gland protection. We thus aimed to study the feasibility of imaging using ICG combined with carbon nanoparticles (CNs) in the identification of sentinel lymph nodes (SLNs) in patients diagnosed with papillary thyroid microcarcinoma (PTMC). METHODS: Two approaches were applied to detect lymph nodes in PTMC surgery. Patients were randomized into 2 groups. ICG and CNs were injected into the thyroid in Group A. In Group B, only CNs was injected. Black-stained or fluorescent nodes observed using near-infrared fluorescence imaging systems were defined as SLNs. SLN and central lymph node (CLN) dissection was completed in both groups. The pathological and postoperative outcomes were compared between 2 groups. RESULTS: There were 40 patients in Group A and 60 in Group B. A total of 138 SLNs were identified; 72 and 66 SLNs were detected and dissected in Groups A and B, respectively. The number of SLNs identified (per patient) in Group A was higher than that in Group B (P = .027). The number of harvested CLNs was 161 and 192 in Groups A and B, respectively, out of which 45 and 48 lymph nodes with metastasis were confirmed by permanent pathology. The CLN metastatic rate in Group A was higher than that in Group B (P = .048). CONCLUSION: Imaging using ICG combined CNs is feasible and safe for SLN identification in PTMC patients. Compared with using only CNs, more SLNs can be removed and more metastatic lymph nodes can be confirmed when using the combined method. Although the combined method appears to accurately stage tumors, further research is needed.


Assuntos
Carbono/administração & dosagem , Carcinoma Papilar/patologia , Verde de Indocianina/administração & dosagem , Excisão de Linfonodo/métodos , Nanopartículas/administração & dosagem , Linfonodo Sentinela/cirurgia , Neoplasias da Glândula Tireoide/patologia , Adulto , Carbono/química , Carcinoma Papilar/cirurgia , Corantes/administração & dosagem , Estudos de Viabilidade , Feminino , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Nanopartículas/química , Imagem Óptica/métodos , Linfonodo Sentinela/patologia , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia/métodos
5.
Braz Oral Res ; 33: e0088, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31531566

RESUMO

The main of the study was quantify the effect of two ceramics with two underlying resin cements on apparent fluorescence levels. Buccal surfaces of two bovine incisors were ground flat producing one enamel and one dentin substrate. The veneers were fabricated (0.5 and 1.0 mm thickness) using two ceramics (IPSe.max Press and IPSe.max Zirpress, Ivoclar Vivadent). Veneers were cemented using either light-cured (Variolink II, Ivoclar Vivadent) or self-adhesive dual (Rely X U200, 3M ESPE) cement. The layered Control group materials had no cement application. Semi-quantitative fluorescence image analysis (Matlabs software, Matworks) involved processing the images as captured under each daylight (DL, Gretagmacbeth) and ultraviolet illuminants (UVA, Sylvania) within a neutral-gray lightbox (Macbeth Spectral Light). Statistical analysis of the quantitative fluorescence values was performed using two-way ANOVA and Tukey's test (p < 0.05). The e.max Zirpress on the dentin substrate produced greater fluorescence (p < 0.05) when subjected to UV illumination and more fluorescence (p < 0.05) than e.max Press in both cement groups. Light-cured cement produced higher (p < 0.05) fluorescence than the dual-cement with e.max Press on enamel under UV illumination. The fluorescence for e.max Press on the dentin substrate was greater (p < 0.05) than for e.max Zirpress using dual self-adhesive cement subjected to daylight illumination. Thus, it is possible to conclude that the combination of ceramic and cement produce definite, significant effects on the apparent fluorescence, vital quality for restorative dentistry.


Assuntos
Cerâmica/química , Facetas Dentárias , Imagem Óptica/métodos , Cimentos de Resina/química , Animais , Bovinos , Esmalte Dentário/química , Esmalte Dentário/efeitos dos fármacos , Dentina/química , Dentina/efeitos dos fármacos , Luz , Teste de Materiais , Valores de Referência , Reprodutibilidade dos Testes , Propriedades de Superfície
6.
Chem Commun (Camb) ; 55(82): 12352-12355, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31559405

RESUMO

We report a new biofunctionalized nanoplatform based on hyaluronic acid-coated gold-nano-dot-decorated hollow carbon nanospheres (AuHCNs-HA) for microRNA imaging in living cells. Importantly, the HA-coated nanoplatform could be internalized into target cells via CD44 receptor-mediated endocytosis. It can be further applied for intracellular miR-21 imaging in CD44-positive colorectal cancer cells.


Assuntos
Neoplasias Colorretais/metabolismo , Ouro/química , Nanopartículas Metálicas/química , MicroRNAs/análise , Imagem Óptica/métodos , Receptores de Hialuronatos/metabolismo , Ácido Hialurônico/química , Imagem Óptica/instrumentação , Tamanho da Partícula , Porosidade , Propriedades de Superfície
7.
Chem Commun (Camb) ; 55(71): 10615-10618, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31428753

RESUMO

A programmable molecular beacon (MB) with a good discrimination capability for mature and precursor microRNAs was loaded onto the surface of Mo2C quantum dots (QDs) for accurate detection of intracellular mature microRNAs.


Assuntos
Corantes Fluorescentes/química , MicroRNAs/análise , Molibdênio/química , Pontos Quânticos/química , Animais , Técnicas Biossensoriais/métodos , Linhagem Celular Tumoral , Humanos , Limite de Detecção , Camundongos , Imagem Óptica/métodos , Precursores de RNA/análise , Propriedades de Superfície
8.
Chem Commun (Camb) ; 55(67): 9967-9970, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31367705

RESUMO

Herein, a seesaw ratiometric (SR) probe is designed which integrates fluorescence and surface enhanced Raman scattering (SERS) technology. Fluorescence imaging enables tracking of the spatiotemporal dynamic behaviour of telomerase. Meanwhile, SERS reverse ratiometric measurement can enable sensitive detection of telomerase activity in single living cells.


Assuntos
Corantes/química , Imagem Óptica/métodos , Análise de Célula Única/métodos , Análise Espectral Raman/métodos , Telomerase/metabolismo , DNA/química , Transferência Ressonante de Energia de Fluorescência/métodos , Ouro/química , Humanos , Células MCF-7 , Nanopartículas Metálicas/química
9.
Chem Commun (Camb) ; 55(67): 10015-10018, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31378791

RESUMO

We report the first neutral and water-soluble polymer capable of strong mitochondrial targeting in vitro and in vivo, zwitterionic poly[2-(N-oxide-N,N-diethylamino)ethyl methacrylate] (OPDEA). OPDEA is quickly internalized via macropinocytosis by various cancer cells and transferred into the mitochondria, which slightly lowers the mitochondrial membrane potential as determined by the JC-1 assay.


Assuntos
Mitocôndrias/metabolismo , Ácidos Polimetacrílicos/química , Animais , Linhagem Celular Tumoral , Corantes Fluorescentes/química , Humanos , Concentração de Íons de Hidrogênio , Potencial da Membrana Mitocondrial , Camundongos , Imagem Óptica/métodos , Permeabilidade , Ácidos Polimetacrílicos/metabolismo , Solubilidade , Água
10.
Chem Commun (Camb) ; 55(73): 10920-10923, 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31441463

RESUMO

The development of new NIR-II fluorophores, particularly those with facile syntheses, high fluorescence quantum yields, and stable and tunable photophysical properties, is challenging. Herein, we report a new class of small molecular NIR-II fluorophores based on aza-dipyrromethene boron difluoride (aza-BODIPY) dyes. We demonstrate promising photophysical properties of these dyes, such as large Stokes shift, superior photostability, and good fluorescence brightness as nanoparticles in aqueous solution. Because of these properties and high resolution and deep penetration NIR-II imaging ability, the aza-BODIPY based dyes show great potential as NIR-II imaging agents.


Assuntos
Compostos de Boro/química , Corantes Fluorescentes/química , Imagem Óptica/métodos , Pirróis/química , Animais , Compostos de Boro/síntese química , Compostos de Boro/efeitos da radiação , Compostos de Boro/toxicidade , Linhagem Celular Tumoral , Teoria da Densidade Funcional , Desenho de Drogas , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Corantes Fluorescentes/toxicidade , Raios Infravermelhos , Camundongos , Modelos Químicos , Nanopartículas/química , Poloxâmero/química , Pirróis/síntese química , Pirróis/efeitos da radiação , Pirróis/toxicidade
11.
Chemistry ; 25(55): 12712-12718, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31433877

RESUMO

Arylated cyclobutanes were accessed by a versatile palladium-catalyzed secondary C(sp3 )-H activation, exploiting chelation assistance by modular triazoles. The C-H arylation led to cyclobutane natural product derivatives in a highly regioselective fashion, setting the stage for the easy access to novel fluorogenic boron-dipyrrin (BODIPY)-labeled probes for live-cell imaging.


Assuntos
Rastreamento de Células/métodos , Ciclobutanos/química , Imagem Óptica/métodos , Triazóis/química , Boro , Compostos de Boro , Catálise , Quelantes/química , Estrutura Molecular , Paládio/química
12.
Nat Methods ; 16(8): 675, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31363210
14.
Chem Commun (Camb) ; 55(64): 9444-9447, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31287465

RESUMO

A new mitochondrion targetable molecular probe for carbon monoxide (CO)-specific detection based on palladium-free mediated opening of spirolactam was designed. The turn-on red fluorescence caused by CO enables a safe and powerful method for unravelling the function of CO in biological systems to be established.


Assuntos
Monóxido de Carbono/análise , Corantes Fluorescentes/química , Mitocôndrias/química , Imagem Óptica/métodos , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Lactamas/química , Espectroscopia de Prótons por Ressonância Magnética , Frações Subcelulares/química
15.
J Food Sci ; 84(8): 2234-2241, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31313313

RESUMO

In order to rapidly and nondestructively identify tea grades, fluorescence hyperspectral imaging (FHSI) technology was proposed in this paper. A total of 309 Tieguanyin tea samples with three different grades were collected and the fluorescence hyperspectral data was acquired by hyperspectrometer (400 to 1000 nm). The characteristic wavelengths were respectively selected by Bootstrapping Soft Shrinkage (BOSS), Variable Iterative Space Shrinkage Approach (VISSA) and Model Adaptive Space Shrinkage (MASS) algorithms. Then, Support Vector Machine (SVM) was applied to establishing the relationship between the characteristic peaks, the full spectra, three characteristic spectra and the labels of tea grades. The results showed that VISSA-SVM model had the best classification performance, but the model precision can still be improved. Thus, Artificial Bee Colony (ABC) algorithm was introduced to optimize the parameters of SVM model. The accuracy and Kappa coefficient of test set of VISSA-ABC-SVM model were improved to 97.436% and 0.962, respectively. Therefore, the combination of fluorescence hyperspectra with VISSA-ABC-SVM model can accurately identify the grade of Tieguanyin tea. PRACTICAL APPLICATION: The rapid and accurate nondestructive tea grade identification method contributes to the construction of the tea online grade detection system. FHSI technology can solve the shortcomings of the reported methods and improved the identification accuracy of tea grades. It can be applied to the rapid detection of tea quality by tea companies, tea market, tea farmers and other demanders.


Assuntos
Algoritmos , Camellia sinensis/química , Imagem Óptica/métodos , Camellia sinensis/classificação , Análise Discriminante , Fluorescência , Folhas de Planta/química , Folhas de Planta/classificação , Máquina de Vetores de Suporte , Chá/química
16.
Biomater Sci ; 7(9): 3855-3865, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31305807

RESUMO

Fluorogens with aggregation-induced emission (AIE) characteristics (AIEgens) possess unique optical properties, design flexibility, and multi-functional capabilities and have established their niche as smart materials since their discovery in 2001. In recent years, AIEgens have found varied applications in sensing, imaging, and therapy in biomedical research. In this work, we systematically and comprehensively investigate the in vitro anticancer activity of AIEgens. We report the high cytotoxicity of AIEgens against cancer cells, especially against cancer stem cells (CSCs) which show high resistance to existing therapeutic drug regimens. Furthermore, we explore the role of AIEgens as novel image-guided chemotherapy agents that offer a new avenue for efficient cancer treatment.


Assuntos
Antineoplásicos/química , Corantes Fluorescentes/química , Fármacos Fotossensibilizantes/química , Estilbenos/química , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Ciclo Celular , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Corantes Fluorescentes/farmacologia , Hemólise , Humanos , Invasividade Neoplásica , Células-Tronco Neoplásicas/citologia , Células-Tronco Neoplásicas/efeitos dos fármacos , Imagem Óptica/métodos , Fotoquimioterapia , Estilbenos/farmacologia , Nanomedicina Teranóstica
17.
Biomater Sci ; 7(9): 3609-3613, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31361290

RESUMO

Development of versatile phototheranostics is highly desirable for cancer theranostics. Herein, a novel organic conjugated polymer (named DPP-TT) with excellent optical properties was designed and prepared. Based on single-component DPP-TT, single DPP-TT NPs as versatile phototheranostics were developed by a simple nanoprecipitation method. The obtained NPs exhibited good water solubility, excellent biocompatibility, outstanding photostability, strong NIR-I light absorption, and appropriate NIR-II fluorescence emission. Importantly, such NPs presented high photothermal conversion efficiency. From the investigations performed in vitro and in vivo, it was observed that DPP-TT NPs show remarkable performance for cancer theranostics, benefiting from single 808 nm laser-induced tri-modal (NIR-II fluorescence/photoacoustic/thermal) imaging-guided photothermal therapy.


Assuntos
Nanopartículas/química , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fototerapia/métodos , Animais , Materiais Biocompatíveis/química , Células HeLa , Humanos , Hipertermia Induzida , Camundongos , Imagem Multimodal/métodos , Imagem Óptica/métodos , Técnicas Fotoacústicas/métodos , Polímeros/química , Solubilidade , Nanomedicina Teranóstica
18.
Isr Med Assoc J ; 21(6): 390-393, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31280507

RESUMO

BACKGROUND: The sentinel lymph node (SLN) biopsy procedure is a well-known method for identifying solid tumors such as breast cancer, vulvar cancer, and melanoma. In endometrial and cervical cancer, SLN has recently gained acceptance. OBJECTIVES: To evaluate the detection rate of SLN with an indocyanine green and near-infrared fluorescent imaging (ICG/NIR) integrated laparoscopic system in clinically uterine-confined endometrial or cervical cancer. METHODS: Patients with clinically early-stage endometrial or cervical cancer were included in this retrospective study. ICG was injected into the uterine cervix and an ICG/NIR integrated laparoscopic system was used during the surgeries. The National Comprehensive Cancer Network (NCCN) protocol was followed. SLN and/or suspicious lymph nodes were resected. Side-specific lymphadenectomy was performed when mapping was unsuccessful. Systematic lymphadenectomy was completed in patients with high-grade histology or deep myometrial invasion. Enhanced pathology using ultra-staging and immunohistochemistry were performed in all cases. RESULTS: We analyzed 46 eligible patients: 39 endometrial and 7 cervical cancers. Of these, 44 had at least one SLN (93.6%). In 41 patients (89%) we detected bilateral SLN, in 3 (7%) only unilateral, and in 2 (4%) none were detected. Seven patients presented with lymph node metastasis. All were detected by NCCN/SLN protocol. Of these cases, two were detected with only pathological ultra-staging. CONCLUSIONS: SLN mapping in endometrial and cervical cancer can easily be performed with a high detection rate by integrating ICG/NIR into a conventional laparoscopic system. Precision medicine in patients evaluated by SLN biopsy changes the way patients with endometrial or cervical cancer are managed.


Assuntos
Neoplasias do Endométrio/patologia , Metástase Linfática/diagnóstico por imagem , Imagem Óptica/métodos , Linfonodo Sentinela/diagnóstico por imagem , Neoplasias do Colo do Útero/patologia , Colo do Útero , Feminino , Humanos , Verde de Indocianina , Laparoscopia , Pessoa de Meia-Idade , Medicina de Precisão , Estudos Retrospectivos
19.
Nat Commun ; 10(1): 3087, 2019 07 12.
Artigo em Inglês | MEDLINE | ID: mdl-31300648

RESUMO

The dorsal root ganglia (DRG) contain the somas of first-order sensory neurons critical for somatosensation. Due to technical difficulties, DRG neuronal activity in awake behaving animals remains unknown. Here, we develop a method for imaging DRG at cellular and subcellular resolution over weeks in awake mice. The method involves the installation of an intervertebral fusion mount to reduce spinal movement, and the implantation of a vertebral glass window without interfering animals' motor and sensory functions. In vivo two-photon calcium imaging shows that DRG neuronal activity is higher in awake than anesthetized animals. Immediately after plantar formalin injection, DRG neuronal activity increases substantially and this activity upsurge correlates with animals' phasic pain behavior. Repeated imaging of DRG over 5 weeks after formalin injection reveals persistent neuronal hyperactivity associated with ongoing pain. The method described here provides an important means for in vivo studies of DRG functions in sensory perception and disorders.


Assuntos
Gânglios Espinais/diagnóstico por imagem , Microscopia Intravital/métodos , Percepção da Dor/fisiologia , Células Receptoras Sensoriais/fisiologia , Animais , Técnicas de Observação do Comportamento/instrumentação , Técnicas de Observação do Comportamento/métodos , Comportamento Animal/fisiologia , Cálcio/química , Feminino , Formaldeído/administração & dosagem , Formaldeído/toxicidade , Gânglios Espinais/citologia , Gânglios Espinais/fisiologia , Proteínas de Fluorescência Verde/química , Proteínas de Fluorescência Verde/genética , Microscopia Intravital/instrumentação , Masculino , Camundongos , Camundongos Transgênicos , Modelos Animais , Imagem Óptica/instrumentação , Imagem Óptica/métodos , Dor/induzido quimicamente , Dor/fisiopatologia , Fótons , Vigília
20.
Ann R Coll Surg Engl ; 101(7): 508-513, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31305127

RESUMO

INTRODUCTION: Near-infrared imaging may facilitate intraoperative identification of parathyroid glands by causing autofluorescence but its clinical value has not been established. Inadvertent parathyroidectomy occurs in 5-22% of thyroidectomies and is associated with temporary and permanent hypoparathyroidism. The aim of this study was to determine whether near-infrared imaging prevents inadvertent parathyroidectomy and early hypocalcaemia as a surrogate for permanent hypoparathyroidism. MATERIALS AND METHODS: Near-infrared imaging was used in a prospective cohort of consecutive thyroidectomies. Thyroidectomies performed prior to the introduction of near-infrared imaging formed a control group. The thyroid bed and specimen were scanned with near-infrared imaging. Areas of autofluorescence on the specimen were examined and any parathyroid tissue found was autotransplanted. Inadvertent parathyroidectomy was therefore recorded as established intraoperatively by near-infrared imaging (allowing autotransplantation) or on subsequent histology (missed). Serum calcium and parathyroid hormone were measured on day one and at two weeks and six months postoperatively. RESULTS: A total of 269 patients were included: 106 near-infrared imaging and 163 controls. Inadvertent parathyroidectomy was detected by near-infrared imaging in two (and autotransplantation performed) and histologically (i.e. missed by near-infrared imaging in 13, 12.3% vs 17, 10.4% controls). Neither result was statistically significant (P = 0.08, 0.89). There was no significant difference in serum calcium or parathyroid hormone between near-infrared imaging and control groups at one day, two weeks or thereafter. DISCUSSION: Near-infrared imaging may detect inadvertent parathyroidectomy and may allow autotransplantation. It did not, however, reduce the incidence of missed inadvertent parathyroidectomy and no difference was seen in early hypocalcaemia or late hypoparathyroidism. Current near-infrared imaging technology does not appear to confer a clinical benefit sufficient to justify its use.


Assuntos
Hipocalcemia/prevenção & controle , Hipoparatireoidismo/prevenção & controle , Complicações Intraoperatórias/prevenção & controle , Imagem Óptica/métodos , Glândulas Paratireoides/diagnóstico por imagem , Tireoidectomia/efeitos adversos , Adulto , Estudos de Viabilidade , Feminino , Fluoroscopia , Humanos , Hipocalcemia/epidemiologia , Hipocalcemia/etiologia , Hipoparatireoidismo/epidemiologia , Hipoparatireoidismo/etiologia , Incidência , Complicações Intraoperatórias/epidemiologia , Complicações Intraoperatórias/etiologia , Masculino , Pessoa de Meia-Idade , Glândulas Paratireoides/lesões , Glândulas Paratireoides/transplante , Estudos Prospectivos , Glândula Tireoide/cirurgia , Transplante Autólogo
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