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1.
Anticancer Res ; 41(9): 4395-4400, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34475059

RESUMO

BACKGROUND/AIM: The aim of this study was to evaluate the cancer detection rate (CDR) using magnetic resonance imaging-transrectal ultrasound (MRI-TRUS) fusion-guided transperineal targeted biopsy (TB). PATIENTS AND METHODS: We included 401 consecutive patients, of which 161 were biopsy-naïve. All underwent prebiopsy bi-parametric MRI; patients with positive MRI [prostate imaging reporting and data system (PI-RADS≥3)] underwent TB. Biopsy-naïve patients with positive MRI underwent TB and systematic biopsies (SBs). MRI-negative patients underwent SBs. Clinically significant prostate cancer (csPCa) was defined as ISUP ≥2. The added value of SB was defined as an upgrade from a negative biopsy or ISUP of 1 in TB to csPCa in SB. RESULTS: The median (interquartile range) age was 69 (range=63-74) years, and PSA was 6.9 (range=4.5-11) ng/ml. The overall CDR was 65%, with csPCa occurring in 48%. In cases of PI-RADS 5, CDR was 91%, and csPCa was 77%. The added value of SB was 2%. CONCLUSION: Transperineal TB biopsies using MRI-TRUS fusion yield a high CDR.


Assuntos
Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico/métodos , Imageamento por Ressonância Magnética Multiparamétrica/métodos , Neoplasias da Próstata/diagnóstico por imagem , Neoplasias da Próstata/patologia , Idoso , Humanos , Masculino , Pessoa de Meia-Idade , Imagem Multimodal , Estudos Prospectivos , Antígeno Prostático Específico/metabolismo , Neoplasias da Próstata/metabolismo , Sensibilidade e Especificidade
2.
Nanoscale ; 13(33): 14049-14066, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34477686

RESUMO

Sonodynamic therapy (SDT) is a highly promising approach for cancer therapy, but its efficacy is severely hampered by the low specificity of sonosensitizers and the unfavorable characteristics of the tumor microenvironment (TME), such as hypoxia and glutathione (GSH) overexpression. To solve these problems, in this work, we encapsulated IR780 and MnO2 in PLGA and linked Angiopep-2 (Ang) to synthesize a multifunctional nanozyme (Ang-IR780-MnO2-PLGA, AIMP) to enhance SDT. With Ang functionalization to facilitate blood-brain barrier (BBB) penetration and glioma targeting, and through the function of IR780, these nanoparticles (NPs) showed improved targeting of cancer cells, especially mitochondria, and spread deep into tumor centers. Upon low-intensity focused ultrasound (LIFU) irradiation, reactive oxygen species (ROS) were produced and induced tumor cell apoptosis. Combined with the specific mitochondria-targeting ability of IR780, the sonodynamic effects were amplified because mitochondria are sensitive to ROS. In addition, MnO2 exhibited enzyme-like activity, reacting with the high levels of hydrogen protons (H+), H2O2 and GSH in the TME to continuously produce oxygen and consume GSH, which further enhanced the effect of SDT. Moreover, Mn2+ can be released in response to TME stimulation and used as a magnetic resonance (MR) contrast agent. In addition, IR780 has photoacoustic (PA)/fluorescence (FL) imaging capabilities. Our results demonstrated that AIMP NPs subjected to LIFU triggering maximally enhanced the therapeutic effect of SDT by multiple mechanisms, including multiple targeting, deep penetration, oxygen supply in situ and GSH depletion, thereby significantly inhibiting tumor growth and distal metastasis without systemic toxicity. In summary, this multifunctional nanozyme provides a promising strategy for cancer diagnosis and treatment under the intelligent guidance of multimodal imaging (PA/FL/MR) and may be a safe clinical translational method.


Assuntos
Nanopartículas , Terapia por Ultrassom , Linhagem Celular Tumoral , Peróxido de Hidrogênio , Compostos de Manganês/farmacologia , Imagem Multimodal , Óxidos , Microambiente Tumoral
3.
Curr Cardiol Rep ; 23(10): 138, 2021 08 19.
Artigo em Inglês | MEDLINE | ID: mdl-34410510

RESUMO

PURPOSE OF THE REVIEW: Percutaneous closure of sinus venosus atrial septal defects (ASD) using covered stent implantation is a new and promising minimally invasive technique. New imaging tools are used to ensure preoperative anatomical characterization and preoperative guidance, which are key procedural success factors. Here we will describe and analyze these recent developments. RECENT FINDINGS: Sinus venosus ASDs present a wide variety of anatomical features which must be described and analyzed using various imaging tools, including 3D technology. Percutaneous closure is challenging, but can hasten clinical recovery compared to the gold-standard conventional open-heart surgery. The feasibility of percutaneous closure relies on precise preoperative anatomical study and on real-time guidance using a multimodal fusion imaging process. Three-dimensional modeling of sinus venosus ASD is essential to understand the large anatomical panel encountered in this pathology. Multimodal fusion imaging guidance is very useful for performing sinus venosus ASD percutaneous closure in selected patients.


Assuntos
Procedimentos Cirúrgicos Cardíacos , Comunicação Interatrial , Cateterismo Cardíaco , Ecocardiografia Transesofagiana , Comunicação Interatrial/diagnóstico por imagem , Comunicação Interatrial/cirurgia , Humanos , Imagem Multimodal , Stents , Resultado do Tratamento
4.
Curr Cardiol Rep ; 23(10): 134, 2021 08 19.
Artigo em Inglês | MEDLINE | ID: mdl-34410523

RESUMO

PURPOSE OF REVIEW: Cardiac amyloidosis is an increasingly recognized condition with a growing range of targeted therapies, but diagnosis requires a high index of suspicion and multimodality imaging expertise. Early diagnosis remains key to improving quality of life and survival. This article reviews the multimodality imaging approach to the diagnosis, differentiation, and prognosis of cardiac amyloidosis. RECENT FINDINGS: Recent advances in multimodality cardiac imaging have allowed for earlier diagnosis of cardiac amyloidosis resulting in earlier initiation of life-saving therapy in cases of light chain amyloidosis and life-prolonging therapy in transthyretin amyloidosis. With these advances in multimodality imaging, it is important for cardiologists and cardiac imagers to be aware of the subtleties of early disease, the appropriate diagnostic approach as well as understanding the practicalities and pitfalls that are encountered with each modality.


Assuntos
Neuropatias Amiloides Familiares , Cardiomiopatias , Cardiomiopatias/diagnóstico por imagem , Diagnóstico Precoce , Coração , Humanos , Imagem Multimodal , Qualidade de Vida
5.
Sensors (Basel) ; 21(16)2021 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-34451117

RESUMO

This paper presents an application of neural networks operating on multimodal 3D data (3D point cloud, RGB, thermal) to effectively and precisely segment human hands and objects held in hand to realize a safe human-robot object handover. We discuss the problems encountered in building a multimodal sensor system, while the focus is on the calibration and alignment of a set of cameras including RGB, thermal, and NIR cameras. We propose the use of a copper-plastic chessboard calibration target with an internal active light source (near-infrared and visible light). By brief heating, the calibration target could be simultaneously and legibly captured by all cameras. Based on the multimodal dataset captured by our sensor system, PointNet, PointNet++, and RandLA-Net are utilized to verify the effectiveness of applying multimodal point cloud data for hand-object segmentation. These networks were trained on various data modes (XYZ, XYZ-T, XYZ-RGB, and XYZ-RGB-T). The experimental results show a significant improvement in the segmentation performance of XYZ-RGB-T (mean Intersection over Union: 82.8% by RandLA-Net) compared with the other three modes (77.3% by XYZ-RGB, 35.7% by XYZ-T, 35.7% by XYZ), in which it is worth mentioning that the Intersection over Union for the single class of hand achieves 92.6%.


Assuntos
Procedimentos Cirúrgicos Robóticos , Robótica , Algoritmos , Humanos , Imageamento Tridimensional , Imagem Multimodal
6.
Radiol Clin North Am ; 59(5): 813-833, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34392921

RESUMO

This review article summarizes the clinical applications of established and emerging PET tracers in the evaluation of the 5 most common gynecologic malignancies: endometrial, ovarian, cervical, vaginal, and vulvar cancers. Emphasis is given to 2-deoxy-2-[18F]fluoro-d-glucose as the most widely used and studied tracer, with additional clinical tracers also explored. The common imaging protocols are discussed, including standard dose ranges and uptake times, established roles, as well as the challenges and future directions of these imaging techniques. The key points are emphasized with images from selected cases.


Assuntos
Neoplasias dos Genitais Femininos/diagnóstico por imagem , Neoplasias dos Genitais Femininos/patologia , Imageamento por Ressonância Magnética/tendências , Imagem Multimodal/tendências , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada/tendências , Feminino , Fluordesoxiglucose F18 , Humanos , Compostos Radiofarmacêuticos
7.
Radiol Clin North Am ; 59(5): 853-874, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34392923

RESUMO

PET/MR imaging is in routine clinical use and is at least as effective as PET/CT for oncologic and neurologic studies with advantages with certain PET radiopharmaceuticals and applications. In addition, whole body PET/MR imaging substantially reduces radiation dosages compared with PET/CT which is particularly relevant to pediatric and young adult population. For cancer imaging, assessment of hepatic, pelvic, and soft-tissue malignancies may benefit from PET/MR imaging. For neurologic imaging, volumetric brain MR imaging can detect regional volume loss relevant to cognitive impairment and epilepsy. In addition, the single-bed position acquisition enables dynamic brain PET imaging without extending the total study length which has the potential to enhance the diagnostic information from PET.


Assuntos
Imageamento por Ressonância Magnética/tendências , Imagem Multimodal/tendências , Tomografia por Emissão de Pósitrons/tendências , Imagem Corporal Total/tendências , Humanos , Compostos Radiofarmacêuticos
8.
ACS Appl Mater Interfaces ; 13(33): 39042-39054, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34375073

RESUMO

In developing a cluster-nanocarrier design, as a magnetic resonance imaging contrast agent, we have investigated the enhanced relaxivity of a manganese and iron-oxo cluster grafted within a porous polystyrene nanobead with increased relaxivity due to a higher surface area. The synthesis of the cluster-nanocarrier for the cluster Mn8Fe4O12(O2CC6H4CH═CH2)16(H2O)4, cross-linked with polystyrene (the nanocarrier), under miniemulsion conditions is described. By including a branched hydrophobe, iso-octane, the resulting nanobeads are porous and ∼70 nm in diameter. The increased surface area of the nanobeads compared to nonporous nanobeads leads to an enhancement in relaxivity; r1 increases from 3.8 to 5.2 ± 0.1 mM-1 s-1, and r2 increases from 11.9 to 50.1 ± 4.8 mM-1 s-1, at 9.4 teslas, strengthening the potential for T1 and T2 imaging. Several metrics were used to assess stability, and the porosity produced no reduction in metal stability. Synchrotron X-ray fluorescence microscopy was used to demonstrate that the nanobeads remain intact in vivo. In depth, physicochemical characteristics were determined, including extensive pharmacokinetics, in vivo imaging, and systemic biodistribution analysis.


Assuntos
Materiais Biocompatíveis/química , Meios de Contraste/química , Ferro/química , Manganês/química , Nanopartículas/química , Compostos Organometálicos/química , Poliestirenos/química , Animais , Materiais Biocompatíveis/farmacocinética , Linhagem Celular Tumoral , Permeabilidade da Membrana Celular , Sobrevivência Celular/efeitos dos fármacos , Meios de Contraste/farmacocinética , Reagentes para Ligações Cruzadas/química , Humanos , Imageamento por Ressonância Magnética , Camundongos Endogâmicos BALB C , Imagem Multimodal , Porosidade , Espectrometria por Raios X , Distribuição Tecidual
9.
BMJ Case Rep ; 14(8)2021 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-34353836

RESUMO

Retinoma or retinocytoma is a spontaneously arrested or spontaneously regressed variant of retinoblastoma. With the advent of the latest non-invasive imaging techniques, it is possible to evaluate the microstructural and microvascular changes associated with this tumour. Although there are a few reports which describe the imaging findings in retinocytoma, information regarding retinocytoma on the multicolour imaging is lacking. Here, we describe the multimodal imaging features in a patient with classic features of retinocytoma with special emphasis on its multicolour imaging features.


Assuntos
Neoplasias Oculares , Doenças Retinianas , Neoplasias da Retina , Retinoblastoma , Humanos , Imagem Multimodal , Neoplasias da Retina/diagnóstico por imagem , Retinoblastoma/diagnóstico por imagem
10.
Medicina (Kaunas) ; 57(7)2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34356978

RESUMO

Neuroretinitis is a rare clinical entity, characterized by optic nerve edema and star-shape hard exudate around fovea. The clinical features include acute unilateral visual loss, dyschromatopsia, relative afferent pupillary defect and visual field abnormalities. Increased vascular permeability of the optic disc is the main pathophysiology. As it is a not fully known clinical entity, diagnosis is challenging. In this case, we use multimodal imaging to reveal pathophysiology and anatomical change of early mild neuroretinitis. Case presentation: A 28-year-old healthy woman presented to the clinic with mild blurred vision in her left eye. After complete ophthalmic examination, outer retinal thickening of the temporal peripapillary area and optic disc edema were observed. Two days after diagnosis, the retinal edema and visual symptoms were aggravated. A hard exudate, maybe a part of macular star, was observed. Multimodal imaging including optical coherence tomography (OCT), swept-source OCT angiography (SS-OCTA), fluorescein angiography, and indocyanine green angiography visualized choroidal thinning and insufficient circulation beneath the outer retinal edema. Following steroid pulse therapy, the retinal edema and blurred vision were completely resolved. Conclusions: Multimodal imaging suggested that unilateral optic disc edema and early macular star help the diagnosis of neuroretinitis. In SS-OCTA, we found focal choroidal insufficiency. The focal insufficient choroidal circulation might be a contribution factor for idiopathic neuroretinitis. Multimodal imaging including SS-OCTA may be a valuable tool for detecting and monitoring disease progression.


Assuntos
Disco Óptico , Retinite , Adulto , Feminino , Angiofluoresceinografia , Humanos , Imagem Multimodal , Retinite/diagnóstico por imagem , Tomografia de Coerência Óptica
11.
Epilepsy Behav ; 122: 108124, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34237676

RESUMO

Sudden death in epilepsy or SUDEP is a fatal condition that accounts for more than 4000 deaths each year. Limited clinical and preclinical data on sudden death suggest critical contributions from autonomic, cardiac, and respiratory pathways. A potential mechanism for such sudden and severe cardiorespiratory dysregulation may be linked to acid reflux-induced laryngospasm. Here, we expand on our previous investigations and utilize a novel multimodal approach to provide visual evidence of acid reflux-initiated cardiorespiratory distress and subsequent sudden death in seizing rats. We used systemic kainic acid to acutely induce seizure activity in Long Evans rats, under urethane anesthesia. We recorded electroencephalography (EEG), electrocardiography (ECG), chest plethysmography, and esophageal pH signals through a multimodal recording platform, during simultaneous fast MRI scans of the rat stomach and esophagus. MRI images, in correlation with electrophysiology data were used to identify seizure progression, stomach acid movement up the esophagus, cardiorespiratory changes, and sudden death. In all cases of sudden death, esophageal pH recordings alongside MRI images visualized stomach acid movement up the esophagus. Severe cardiac (ST segment elevation), respiratory (intermittent apnea) and brain activity (EEG narrowing due to hypoxia) changes were observed only after acid reached larynx, which strongly suggested onset of laryngospasm following acid reflux. The complementary information coming from electrophysiology and fast MRI scans provided insight into the mechanism of esophageal reflux, laryngospasm, obstructive apnea, and subsequent sudden death in seizing animals. The results carry clinical significance as it outlines a potential mechanism that may be relevant to SUDEP in humans.


Assuntos
Epilepsia , Morte Súbita Inesperada na Epilepsia , Animais , Morte Súbita/etiologia , Imagem Multimodal , Ratos , Ratos Long-Evans
12.
Adv Healthc Mater ; 10(16): e2100656, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34212539

RESUMO

AGuIX are emerging radiosensitizing nanoparticles (NPs) for precision radiotherapy (RT) under clinical evaluation (Phase 2). Despite being accompanied by MRI thanks to the presence of gadolinium (Gd) at its surface, more sensitive and quantifiable imaging technique should further leverage the full potential of this technology. In this study, it is shown that 89 Zr can be labeled on such NPs directly for positron emission tomography (PET) imaging with a simple and scalable method. The stability of such complexes is remarkable in vitro and in vivo. Using a glioblastoma orthotopic rat model, it is shown that injected 89 Zr-AGuIX is detectable inside the tumor for at least 1 week. Interestingly, the particles seem to efficiently infiltrate the tumor even in necrotic areas, which places great hope for the treatment of radioresistant tumor. Lastly, the first PET/MR whole-body imaging is performed in non-human primate (NHP), which further demonstrates the translational potential of these bimodal NP.


Assuntos
Glioblastoma , Nanopartículas , Animais , Meios de Contraste , Glioblastoma/diagnóstico por imagem , Humanos , Macaca , Imageamento por Ressonância Magnética , Imagem Multimodal , Ratos
14.
Methods Mol Biol ; 2350: 105-123, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34331282

RESUMO

Early detection of malignant tumors, micrometastases, and disseminated tumor cells is one of the effective way of fighting cancer. Among the many existing imaging methods like computed tomography (CT), ultrasound (US), magnetic resonance imaging (MRI), positron emission tomography (PET), and single-photon emission computed tomography (SPECT), optical imaging with fluorescent probes is one of the most promising alternatives because it is fast, inexpensive, safe, sensitive, and specific. However, traditional fluorescent probes, based on organic fluorescent dyes, suffer from the low signal-to-noise ratio. Furthermore, conventional organic fluorescent dyes are unsuitable for deep tissue imaging because of the strong visible light absorption by biological tissues. The use of fluorescent semiconductor nanocrystals, or quantum dots (QDs), may overcome this limitation due to their large multiphoton cross section, which ensures efficient imaging of thick tissue sections inaccessible with conventional fluorescent probes. Moreover, the lower photobleaching and higher brightness of fluorescence signals from QDs ensures a much better discrimination of positive signals from the background. The use of fluorescent nanoprobes based on QDs conjugated to uniformly oriented high-affinity single-domain antibodies (sdAbs) may significantly increase the sensitivity and specificity due to better recognition of analytes and deeper penetration into tissues due to small size of such nanoprobes.Here, we describe a protocol for the fabrication of nanoprobes based on sdAbs and QDs, preparation of experimental xenograft mouse models for quality control, and multiphoton imaging of deep-tissue solid tumors, micrometastases, and disseminated tumor cells.


Assuntos
Imunofluorescência/métodos , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Neoplasias/diagnóstico por imagem , Neoplasias/patologia , Pontos Quânticos , Anticorpos de Domínio Único , Linhagem Celular Tumoral , Imunofluorescência/normas , Humanos , Imunoconjugados/química , Imuno-Histoquímica/métodos , Sondas Moleculares , Imagem Multimodal/métodos , Nanopartículas , Micrometástase de Neoplasia , Imagem Óptica/métodos
15.
J Card Surg ; 36(10): 3862-3864, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34216031

RESUMO

We report a rare clinical case of multilobulated subvalvular aortic aneurysm located in the left ventricular outflow tract, involving the membranous ventricular septum, and associated with mild prolapse of the noncoronary aortic leaflet, emphasizing the importance of multimodality imaging approach, and multidisciplinary discussion.


Assuntos
Aneurisma Aórtico , Septo Interventricular , Aneurisma Aórtico/diagnóstico por imagem , Aneurisma Aórtico/cirurgia , Ecocardiografia , Humanos , Imagem Multimodal , Septo Interventricular/diagnóstico por imagem , Septo Interventricular/cirurgia
16.
Echocardiography ; 38(8): 1474-1477, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34286882

RESUMO

A primary cardiac angiosarcoma (PCA) is very rare, highly aggressive, and metastatic in nature. The manifestations are often nonspecific, and the overall prognosis is extremely poor. The diagnosis of PCA can be complex and remains challenging. The key for diagnosis is echocardiography, and multimodality imaging is a more advantageous modality. Here, we present a rare case of a 41-year-old man who was diagnosed with PCA with multiple lung and bone metastases using multimodal imaging technology. Our case emphasizes the value of multimodality imaging in diagnosing PCA and suggests that patients showing intra-cardiac thrombus and hemorrhagic effusion on echocardiography without a clear explanation should be further investigated or closely followed.


Assuntos
Neoplasias Cardíacas , Hemangiossarcoma , Adulto , Ecocardiografia , Neoplasias Cardíacas/diagnóstico por imagem , Hemangiossarcoma/diagnóstico por imagem , Humanos , Masculino , Imagem Multimodal , Prognóstico
18.
BMC Ophthalmol ; 21(1): 284, 2021 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-34301208

RESUMO

BACKGROUND: To report the first Italian case of hypotrichosis with juvenile macular dystrophy complicated by macular neovascularization diagnosed through multimodal imaging. CASE PRESENTATION: An 11-year-old boy was referred to our Institution for bilateral maculopathy of unknown origin. Multimodal imaging helps the diagnosis of Juvenile Macular Dystrophy with Hypotrichosis (HJMD). Fundus examination showed several alterations of the retinal pigment epithelium and circular pigmented area of chorioretinal atrophy. Structural spectral domain optical coherence tomography (OCT) showed some backscattering phenomenon with several alterations of retinal pigment epithelium and photoreceptor layer in both eyes. Moreover, OCT showed hyperreflective lesion beneath the neuroepithelium in left eye. OCT angiography (OCT-A) revealed a pathologic neovascular network in choriocapillaris plexus, probably the result of a fibrovascular membrane. Multifocal electroretinograms (MfERGs) showed functional alterations in 12.22° of the central retina. In order to confirm the suspicion of HJMD, the child and both parents underwent genetic testing. Both parents resulted to be heterozygous healthy carriers of a single variation. CONCLUSION: Multimodal imaging, in particular OCT-A, is a useful aid, along to clinical findings and genetics, for the diagnosis of inherited retinal dystrophies.


Assuntos
Hipotricose , Degeneração Macular , Distrofias Retinianas , Criança , Angiofluoresceinografia , Humanos , Masculino , Imagem Multimodal , Distrofias Retinianas/diagnóstico , Distrofias Retinianas/genética , Epitélio Pigmentado da Retina , Tomografia de Coerência Óptica
19.
Biomater Sci ; 9(16): 5652-5664, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34259244

RESUMO

Pathological angiogenesis is a critical contributor to atherosclerotic plaque rupture. However, there are few effective theranostic strategies to stabilize plaques by suppressing neovascularization. In this study, we fabricated a polymeric nanosystem using 3 nm manganese ferrite (MnFe2O4) and perfluorohexane (PFH) stabilized by polylactic acid-glycolic acid (PLGA) shells and conjugated to the surface of an anti-vascular endothelial growth factor receptor 2 (VEGFR2) antibody [ramucirumab (Ram)]. The PFH@PLGA/MnFe2O4-Ram nanoparticles (NPs) were used as atherosclerotic plaque angiogenesis theranostics for multimodal imaging-guided photothermal therapy (PTT). Three-nanometer MnFe2O4 is an excellent magnetic resonance imaging T1 and photoacoustic imaging contrast agent. Upon exposure to near-infrared (NIR) light, MnFe2O4 in the NPs could transform NIR light into thermal energy for the photothermal elimination of plaque angiogenesis. Additionally, optical droplet vaporization of PFH in the NPs triggered by the thermal effect to form gas bubbles enhanced ultrasound imaging. Our in vitro experiments revealed that PFH@PLGA/MnFe2O4-Ram NPs actively accumulated in rabbit aortic endothelial cells, and NP-mediated PTT promoted endothelial cell apoptosis while inhibiting their proliferation, migration, and tubulogenesis. Notably, the PFH@PLGA/MnFe2O4-Ram NPs possessed excellent photostability and biocompatibility. In the rabbit advanced atherosclerotic plaque model, PFH@PLGA/MnFe2O4-Ram NP-guided PTT significantly induced apoptosis of neovascular endothelial cells and improved the hypoxia status in the plaque 3 days after treatment. On day 28, PTT significantly reduced the density of neovessels and subsequently stabilized rabbit plaques by inhibiting plaque hemorrhage and macrophage infiltration. Collectively, these results suggest that PFH@PLGA/MnFe2O4-Ram NP-guided PTT is a safe and effective theranostic strategy for inhibiting atherosclerotic plaque angiogenesis.


Assuntos
Nanopartículas , Placa Aterosclerótica , Animais , Linhagem Celular Tumoral , Células Endoteliais , Compostos Férricos , Imagem Multimodal , Neovascularização Patológica/diagnóstico por imagem , Neovascularização Patológica/tratamento farmacológico , Fototerapia , Placa Aterosclerótica/diagnóstico por imagem , Coelhos , Nanomedicina Teranóstica
20.
EBioMedicine ; 69: 103462, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34229278

RESUMO

BACKGROUND: Gastric inflammation is a major risk factor for gastric cancer. Current endoscopic methods are not able to efficiently detect and characterize gastric inflammation, leading to a sub-optimal patients' care. New non-invasive methods are needed. Reflectance mucosal light analysis is of particular interest in this context. The aim of our study was to analyze reflectance light and specific autofluorescence signals, both in humans and in a mouse model of gastritis. METHODS: We recruited patients undergoing gastroendoscopic procedure during which reflectance was analysed with a multispectral camera. In parallel, the gastritis mouse model of Helicobacter pylori infection was used to investigate reflectance from ex vivo gastric samples using a spectrometer. In both cases, autofluorescence signals were measured using a confocal microscope. FINDINGS: In gastritis patients, reflectance modifications were significant in near-infrared spectrum, with a decrease between 610 and 725 nm and an increase between 750 and 840 nm. Autofluorescence was also modified, showing variations around 550 nm of emission. In H. pylori infected mice developing gastric inflammatory lesions, we observed significant reflectance modifications 18 months after infection, with increased intensity between 617 and 672 nm. Autofluorescence was significantly modified after 1, 3 and 6 months around 550 and 630 nm. Both in human and in mouse, these reflectance data can be considered as biomarkers and accurately predicted inflammatory state. INTERPRETATION: In this pilot study, using a practical measuring device, we identified in humans, modification of reflectance spectra in the visible spectrum and for the first time in near-infrared, associated with inflammatory gastric states. Furthermore, both in the mouse model and humans, we also observed modifications of autofluorescence associated with gastric inflammation. These innovative data pave the way to deeper validation studies on larger cohorts, for further development of an optical biopsy system to detect gastritis and finally to better surveil this important gastric cancer risk factor. FUNDING: The project was funded by the ANR EMMIE (ANR-15-CE17-0015) and the French Gastroenterology Society (SNFGE).


Assuntos
Gastrite/diagnóstico por imagem , Gastroscopia/métodos , Imagem Multimodal/métodos , Imagem Óptica/métodos , Adulto , Idoso , Animais , Feminino , Fluorescência , Gastrite/microbiologia , Gastrite/patologia , Helicobacter pylori/patogenicidade , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Imagem Multimodal/instrumentação , Imagem Óptica/instrumentação , Gravação em Vídeo/métodos
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