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1.
Pharm Res ; 37(3): 35, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31950282

RESUMO

PURPOSE: Epicutaneous immunotherapy (EPIT) involving the skin's immune system is easy to use, painless and has a low risk of systemic side effects; it can be applied to food allergies that have a high morbidity rate in children. In this study, we evaluated the safety and efficacy of hydrophilic gel patch (HG) for EPIT. METHODS: Milk protein concentrate (MPC)-containing HG was applied to the skin that maintained a barrier function or formed puncture holes with microneedle, and MPC-specific antibodies were measured. The clinical study was conducted involving patients with severe milk allergy. RESULTS: No specific immune response was induced when immunizing to intact skin, and antibody production was observed by forming puncture holes. It was suggested that MPC contained in HG has immunogenicity and a very small amount of MPC was delivered to intact skin. In the clinical study, the symptom induction threshold increased in four of eight subjects, allowing them to consume milk and switch to oral immunotherapy. Although local skin reactions and temporary elevation of specific IgE antibodies were observed, no systemic side effects appeared throughout the study. CONCLUSIONS: EPIT using HG is a safe method to enable oral administration even in patients with severe milk allergies.


Assuntos
Resinas Acrílicas/química , Imunoglobulina E/imunologia , Hipersensibilidade a Leite/imunologia , Proteínas do Leite/imunologia , Adesivo Transdérmico , Administração Cutânea , Animais , Bovinos , Criança , Pré-Escolar , Modelos Animais de Doenças , Feminino , Humanos , Interações Hidrofóbicas e Hidrofílicas , Imunoglobulina E/análise , Imunoterapia , Masculino , Camundongos , Microinjeções , Proteínas do Leite/administração & dosagem , Agulhas , Projetos Piloto , Pele/metabolismo , Resultado do Tratamento
2.
Allergol. immunopatol ; 47(6): 579-584, nov.-dic. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-186551

RESUMO

Background: In Portugal, the pollen types most implicated in respiratory allergy are grasses, olive and parietaria. The knowledge of sensitizations to molecular allergens in children and adults can contribute to better diagnosis and treatment of this pathology. Methods: ImmunoCAP singleplex technology was used for molecular allergens and Phadia 250(R) automatic equipment. g205 (Phl p1); g215 (Phl p5b); g210 (Phl p7); and g212 (Phl p12) allergen determinations were made in 45 patients with positive grass sensitization tests. Results: The majority of patients are sensitized to Phl p1 (91%) and Phl p1+/Phl p5-/Phl p7-/Phl p12- was the most dominant profile (40%). In the adult group, the IgE averages for Phl p1 were approximately 10.46, while they were 8.43 for Phl p5, 0.69 for Phl p7, and 0.06 for Phl p12. In the child group, these values were higher: 22.49, 20.23, 3.89, and 0.35, respectively. For allergens Phl p1, Phl p5, and Phl p7, these differences between the child and adult population were not statistically significant (p = 0.754, p = 0.806 and p = 0.102, respectively), but for Phl p12, a statistically significant difference (p = 0.018) was observed. Conclusions: IgE antibodies Phl p1 is the most important allergic marker and sensitivities caused by Phl p12 give rise to higher IgE values in children


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Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Alérgenos/administração & dosagem , Alérgenos/imunologia , Imunização/métodos , Pólen/classificação , Técnicas de Imunoadsorção , Hipersensibilidade/diagnóstico , Imunoglobulina E/análise , Portugal , Hipersensibilidade/imunologia , Pólen/imunologia , Imunoterapia
3.
Medicina (Kaunas) ; 55(8)2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31405131

RESUMO

Background and Objectives: Cow's milk protein allergy (CMA) is the most common allergy in children. The natural history of CMA is generally favorable and the majority of children reach tolerance during childhood, even if studies show variable results. Atopic dermatitis (AD) is a complex disease from an immunological point of view. It is characterized by an impaired skin barrier function and is often the first clinical manifestation of the so-called "atopic march". The aim of our study is to evaluate, in a cohort of children with CMA, if the presence of AD in the first months of life can influence the atopic status of patients, the tolerance acquisition to cow's milk, the level of specific IgE (sIgE), and the sensitization towards food and/or inhalant allergens. Materials and Methods: We enrolled 100 children with a diagnosis of CMA referred to our Pediatric Allergology Unit, aged 1-24 months at the time of the first visit. Results: 71 children had AD and 29 did not. The mean follow-up was 5.28 years. The CMA manifestations were mainly cutaneous, especially in children with AD (91.6% vs. 51.7%; P < 0.001). Patients with AD showed higher rates of polysensitization to foods and higher levels of both total IgE and sIgE for milk, casein, wheat, peanuts, and cat dander at different ages when compared to patients without AD. We analyzed the presence of IgE sensitization for the main foods and inhalants at various ages in the two groups of patients: a statistically significant difference emerged in the two groups of patients for milk, yolk and egg white, hazelnut, peanuts, soybean, grass pollen and cat dander. Meanwhile, we did not find significant differences in terms of tolerance acquisition toward cow's milk, which was nonetheless reached around 5 years of age in 61% of patients. The level of cow's milk sIgE at the age of 5 years was significantly higher in the group of patients who did not acquire tolerance (38.38 vs. 5.22 kU/L; P < 0.0001). Conclusions: An early barrier deficiency appears to promote the development of allergic sensitization, but does not seem to influence the acquisition of tolerance.


Assuntos
Dermatite Atópica/complicações , Hipersensibilidade a Leite/etiologia , Animais , Bovinos , Criança , Pré-Escolar , Dermatite Atópica/imunologia , Dermatite Atópica/fisiopatologia , Humanos , Imunoglobulina E/análise , Imunoglobulina E/sangue , Lactente , Leite/efeitos adversos , Leite/imunologia , Hipersensibilidade a Leite/imunologia , Hipersensibilidade a Leite/fisiopatologia
4.
Medicina (Kaunas) ; 55(7)2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31340608

RESUMO

Gluten-related disorders are very common in pediatric patients. Wheat allergy is triggered by an immunoglobulin E (IgE)-dependent mechanism; its prevalence varies according to the age and region, and in Europe has been estimated to be lower than 1%. Many studies investigated the potential role of several external factors that can influence the risk to developing wheat allergy, but results are still inconclusive. It can be responsible for several clinical manifestations depending on the route of allergen exposure: food-dependent exercise-induced anaphylaxis (FDEIA), occupational rhinitis or asthma (also known as baker's asthma), and contact urticaria. The prognosis of IgE-mediated wheat allergy in children is generally favorable, with the majority of children becoming tolerant by school age. Patients who experienced an anaphylactic reaction prior to 3 years of age and patients with higher level of wheat- or ω-5 gliadin-specific IgE antibodies seem to be at higher risk of persistent wheat allergy. The current management of patients is dietary avoidance. Nowadays, oral immunotherapy has been proposed for wheat allergy with promising results, even if further studies are necessary to establish the best protocol in order to promote tolerance in wheat-allergic children.


Assuntos
Hipersensibilidade a Trigo/fisiopatologia , Criança , Pré-Escolar , Europa (Continente)/epidemiologia , Feminino , Humanos , Imunoglobulina E/efeitos adversos , Imunoglobulina E/análise , Imunoterapia/métodos , Imunoterapia/tendências , Masculino , Triticum/efeitos adversos , Triticum/imunologia , Hipersensibilidade a Trigo/epidemiologia
5.
PLoS Negl Trop Dis ; 13(6): e0007415, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31170141

RESUMO

BACKGROUND: Strongyloides stercoralis is an intestinal nematode unique in its ability to replicate in the human host, allowing ongoing cycles of autoinfection, persisting for decades within the same host. Although usually asymptomatic, overwhelming infections can occur in Strongyloides and HTLV-1 co-infected individuals (SS/HTLV-1). Regulatory T cells (Tregs) are able to blunt specific Th2 responses necessary to control the parasite. We previously reported that peripheral blood Tregs are increased in SS/HTLV-1 and correlate with low Th2 responses. We hypothesized that Tregs are also increased at the site of infection in duodenal mucosa. METHODS: Paraffin embedded duodenal biopsies were obtained from 10 SS/HTLV-1 patients, 3 controls with non-parasitic chronic duodenitis, and 2 healthy controls. Immunohistochemistry was performed using monoclonal antibodies against human CD3, CD8, IgE and FoxP3. The number of cells were counted using a conventional light microscope. The number of CD3+, CD8+, FoxP3+ and IgE positive cells per 0.35 mm2 was measured using ImagePro Plus software comparing areas adjacent or distant from parasite material. RESULTS: In patients with SS/HTLV-1, T lymphocyte counts and CD8+ cells were lower in areas adjacent to the parasite compared to non-adjacent areas (CD3+: adjacent: 6.5 [Interquartile range (IQR: 2.8-12.3)]; non-adjacent: 24.5 [IQR: 20.9-34.4]; Mann-Whitney p = 0.0003; CD8+: adjacent: 4.5 [IQR: 2.3-11.8]; non-adjacent: 21 [IQR: 15.3-42.9]; Mann-Whitney p = 0.0011). Tregs cells in the intestines (FoxP3+ expressing cells) were increased in patients with SS/HTLV-1 compared with patients with chronic duodenitis (SS/HTLV-1: 1.5 [IQR: 0.7-2.3]; duodenitis controls: 0 [range 0-0.7]; healthy controls: 0; Mann-Whitney p = 0.034). There was also a trend towards fewer eosinophils adjacent to the parasites. Among SS/HTLV-1 patients the number of IgE expressing cells was increased for in areas not adjacent to the parasite compared to non-adjacent areas (ANOVA, p = 0.001). CONCLUSIONS: Our data shows increased Treg cell numbers localized adjacent to the parasites in the duodenum SS/HTLV-1 patients. In addition, other T lymphocytes and IgE expressing cells were decreased adjacent to the parasites, suggesting an important role for Tregs in down-regulating local parasite effector responses.


Assuntos
Coinfecção/patologia , Duodeno/patologia , Infecções por HTLV-I/patologia , Imunoglobulina E/análise , Fatores Imunológicos/análise , Estrongiloidíase/patologia , Linfócitos T Reguladores/imunologia , Adolescente , Adulto , Biópsia , Coinfecção/complicações , Feminino , Expressão Gênica , Infecções por HTLV-I/complicações , Humanos , Imuno-Histoquímica , Mucosa Intestinal/patologia , Masculino , Pessoa de Meia-Idade , Estrongiloidíase/complicações , Adulto Jovem
6.
Nanoscale ; 11(26): 12573-12581, 2019 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-31219127

RESUMO

Quantifying interactions between biomolecules subject to various environmental conditions is essential for applications such as drug discovery and precision medicine. This paper presents an investigation of the kinetics of environmentally dependent biomolecular binding using an electrolyte-gated graphene field-effect transistor (GFET) nanosensor. In this approach, biomolecular binding occurring on and in the vicinity of a graphene surface induces a change in carrier concentration, whose resulting conductance change is measured. This allows a systematic study of the kinetic properties of the binding system. We apply this approach to the specific binding of human immunoglobulin E (IgE), an antibody involved in parasite immunity, with an aptamer at different ionic strengths (Na+ and Mg2+) and temperatures. Experimental results demonstrate increased-rate binding kinetics at higher salt-ion concentrations and temperatures. In particular, the divalent cation Mg2+ yields more pronounced changes in the conformational structure of the aptamer than the monovalent cation Na+. In addition, the dissociation of the aptamer-protein complex at room temperature is found to be characterized by large unfavorable changes in the activation enthalpy and entropy.


Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais , Grafite/química , Imunoglobulina E/análise , Transistores Eletrônicos , Humanos , Cinética
7.
Rhinology ; 57(4): 313-320, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31129685

RESUMO

BACKGROUND: The diagnostic value of serum specific Immunoglobulin E (sIgE) and nasal allergen provocation test (NAPT) has been well investigated in local allergic rhinitis (LAR). We hypothesized that nasal local sIgE could be used for the diagnosis of LAR instead of NAPT. METHODS: This was a prospective single center study. Overall, 212 chronic rhinitis patients were screened, of whom 73 were recruited based on negative findings for serum IgE and positive findings for local eosinophils. Ten healthy subjects were also recruited as controls. All participants completed questionnaires at recruitment to record their demographic data, nasal symptom severity, and physician-diagnosed comorbid asthma. Symptom severity was recorded using a visual analogue scale (VAS) of 10 cm and allergic status was assessed by serum sIgE. Nasal secretions were collected for analysis of local sIgE and eosinophils, and NAPT was performed for confirmation of LAR. RESULTS: Overall, 14 patients demonstrated positive local sIgE results. Twelve of these patients had significantly higher local sIgE levels compared to controls, and also demonstrated positive NAPT results. The VAS scores, nasal airway resistance measured by active rhinomanometry, and the levels of local sIgE, ECP, histamine and leukotriene C4 were significantly increased from baseline values following NAPT. Sensitivity, specificity, and diagnostic accuracy of local sIgE for diagnosis of LAR were 91.7% respectively. CONCLUSIONS: The measurement of local sIgE levels in nasal secretion is a reliable and effective diagnostic method for LAR.


Assuntos
Imunoglobulina E , Rinite Alérgica , Alérgenos , Secreções Corporais/química , Humanos , Imunoglobulina E/análise , Testes de Provocação Nasal , Nariz/química , Estudos Prospectivos , Rinite Alérgica/diagnóstico
8.
PLoS One ; 14(4): e0214745, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30995240

RESUMO

Given the growing incidence and prevalence of life-threatening food allergies, health concerns have raised new perspectives for in vivo and in vitro diagnostic methodologies, pointing to saliva as a promising material, already used to diagnose other pathologies. Based on the above considerations, this study aimed to verify the possible use of saliva for the detection of IgE and IgG1 in the diagnosis of food allergy. This was a randomized, cross-sectional clinical study with a quantitative approach, developed at a hospital referral center in allergy in the state of Ceará, from January to July 2015. The sample consisted of 36 children of both sexes, aged between 1 and 60 months, with a diagnosis of cow's milk protein allergy (CMPA) by the RAST test. Children hospitalized or under immunosuppressive drugs were excluded from the study. Serum and saliva samples of the participants were collected and subsequently subjected to the indirect immunoenzymatic assay (ELISA) for the detection of specific serum and salivary immunoglobulins for food: corn, papaya, cow's milk, egg white, wheat, soybeans, peanuts, nuts, kiwi, cacao, fish, shrimp, bananas and tomatoes. For comparison of serum and saliva results, the T-test of independent samples and Mann-Whitney were adopted, for samples with normal and non-normal distribution respectively. A confidence interval of 95% was adopted for significant results. It was observed that 100% (n = 36) of the participants presented cow's milk allergy through the indirect ELISA, detecting IgE or IgG1 in serum and saliva. When serum IgE and IgG1 concentrations were compared, there was no statistical difference (p > 0.05) in 12 of the 14 foods evaluated. The same amount (n = 12) of non-significant differences (p > 0.05) was observed in the comparison of the 14 foods under IgE and IgG1 contractions in saliva. In the verification of the average values of IgE present in the serum and saliva of the foods, only cow's milk, fish and papaya showed statistically significant differences (p < 0.05). Of the total food evaluated, only the average levels of IgG1 present in serum and saliva showed a significant value (p < 0.05) in banana and tomato. These findings indicate that the detection of IgE and IgG1 in saliva proves to be as efficient as in the serum. The use of the salivary technique for use in the diagnosis of food allergy is suggested.


Assuntos
Hipersensibilidade Alimentar/diagnóstico , Imunoglobulina E/análise , Imunoglobulina G/análise , Saliva/metabolismo , Animais , Bovinos , Pré-Escolar , Estudos Transversais , Feminino , Hipersensibilidade Alimentar/tratamento farmacológico , Humanos , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Imunossupressores/uso terapêutico , Lactente , Recém-Nascido , Masculino , Leite/imunologia , Leite/metabolismo , Hipersensibilidade a Leite/diagnóstico , Hipersensibilidade a Leite/tratamento farmacológico , Estatísticas não Paramétricas
9.
Clin Rev Allergy Immunol ; 57(2): 261-271, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30734159

RESUMO

Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergy that has been well-characterized clinically, yet it is still poorly understood. Acute FPIES is characterized by vomiting 1-4 h and/or diarrhea within 24 h after ingestion of a culprit food. Chronic FPIES is the result of chronic exposure to an offending food that can result in chronic watery diarrhea, intermittent vomiting, and failure to thrive. FPIES typically presents in infancy and self-resolves by school age in most patients. Adult-onset FPIES is rare, but it has been reported. Cow's milk and soy are the most common triggering foods in infants in the US, and as solids are introduced in the diet, FPIES reactions to grains (rice, oat) increase in prevalence. Variability in common trigger foods exists depending on the geographical origin-for example, fish is a frequent trigger in Spanish and Italian patients. Heavy reliance on a detailed history is required for the diagnosis as physical exam findings, laboratory tests, and/or imaging studies are suggestive and not specific for FPIES. Oral food challenges remain the gold standard for confirming diagnosis, and the challenge protocol may be for an individual depending on risk of reaction, prior reaction severity, and positive-specific IgE status. The recent development of diagnostic criteria in 2017 will serve to increase recognition of the disorder and allow for early implementation of management strategies. Acute management during reactions includes IV hydration, anti-emetics, and IV corticosteroids. Reaction prevention strategies include strict food avoidance until the physician deems a food reintroduction challenge clinically appropriate. Future efforts in FPIES research should be aimed at elucidating the underlying disease mechanisms and possible treatment targets.


Assuntos
Enterocolite/epidemiologia , Enterocolite/imunologia , Hipersensibilidade Alimentar/epidemiologia , Hipersensibilidade Alimentar/imunologia , Proteínas/imunologia , Adolescente , Corticosteroides/uso terapêutico , Adulto , Alérgenos/imunologia , Antieméticos/uso terapêutico , Criança , Pré-Escolar , Diagnóstico Diferencial , Dieta , Enterocolite/diagnóstico , Enterocolite/terapia , Feminino , Hidratação , Humanos , Imunoglobulina E/análise , Incidência , Lactente , Masculino , Ondansetron/uso terapêutico , Testes do Emplastro
10.
Forensic Sci Int ; 297: e11-e13, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30772126

RESUMO

Postmortem biochemical investigations in vitreous humor samples collected before and after performing multiphase postmortem computed tomography angiography were performed in the past and demonstrated that specific contrast material injection allowed perfusion and radiological identification of the main vessels of the eye to be obtained without any changes in vitreous humor composition. In the study presented herein, we aimed to test whether the injection of the same contrast material using the same postmortem angiography protocol might influence pericardial fluid composition. Postmortem biochemical investigations were performed on pericardial fluid samples collected from bodies that underwent postmortem angiography (n = 16) prior to and post angiography. Two pericardial fluid samples were analyzed. No statistically significant differences were noticed among levels of any tested markers (urea nitrogen, creatinine, uric acid, C-reactive protein, procalcitonin, beta-hydroxybutyrate, and total IgE levels) in pericardial fluid samples collected prior to and post angiography, leading to the conclusion that pericardial fluid sampling can be delayed until after postmortem angiography when a specific contrast material injection is used.


Assuntos
Angiografia , Meios de Contraste , Líquido Pericárdico/química , Mudanças Depois da Morte , Ácido 3-Hidroxibutírico/análise , Nitrogênio da Ureia Sanguínea , Proteína C-Reativa/análise , Creatinina/análise , Feminino , Humanos , Imunoglobulina E/análise , Masculino , Pessoa de Meia-Idade , Pró-Calcitonina/análise , Estudos Prospectivos , Ácido Úrico/análise
11.
Occup Environ Med ; 76(4): 269-278, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30804164

RESUMO

OBJECTIVES: To determine the test performance parameters for the retrievable range of high-molecular-weight (HMW) and low-molecular-weight (LMW) occupational allergens and to evaluate the impact of allergenic components and the implementation of measures for test validation. METHODS: A protocol with predefined objectives and inclusion criteria was the basis of an electronic literature search of MEDLINE and EMBASE (time period 1967-2016). The specific inhalation challenge and serial peak flow measurements were the reference standards for the specific IgE (sIgE) test parameters. All of the review procedures were reported according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses. RESULTS: Seventy-one studies were selected, and 62 entered meta-analysis. Pooled pairs analysis indicated a sensitivity of 0.74(95% CI 0.66 to 0.80) and specificity of 0.71(95% CI 0.63 to 0.77) for HMW allergens and a sensitivity of 0.28(95% CI 0.18 to 0.40) and specificity of 0.89(95% CI 0.77 to 0.95) for LMW allergens. Component-specific analysis improved the test parameters for some allergens. Test validation was handled heterogeneously among studies. CONCLUSION: sIgE test performance is rather satisfactory for a wide range of HMW allergens with the potential for component-specific approaches, whereas sensitivity for LMW allergens is considerably lower, indicating methodological complications and/or divergent pathomechanisms. A common standard for test validation is needed.


Assuntos
Alérgenos/imunologia , Asma Ocupacional/sangue , Asma Ocupacional/diagnóstico , Imunoglobulina E/análise , Alérgenos/metabolismo , Animais , Área Sob a Curva , Biomarcadores/análise , Bovinos , Grão Comestível/imunologia , Grão Comestível/metabolismo , Humanos , Imunoglobulina E/sangue , Hipersensibilidade ao Látex/diagnóstico , Hipersensibilidade ao Látex/imunologia , Curva ROC
12.
Am J Rhinol Allergy ; 33(2): 178-183, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30656948

RESUMO

BACKGROUND: Specific immunoglobulin E (sIgE) within the nasal airway is likely to be the most ideal marker of allergic status, but little is known of the normative values in asymptomatic patients and those with rhinitis. OBJECTIVE: The aim of this study was to assess the diagnostic characteristics of inferior turbinate tissue biopsy sIgE in asymptomatic and rhinitic patients. METHODS: A diagnostic cross-sectional study was undertaken, involving patients who underwent inferior turbinate surgery with or without other surgical interventions. Inferior turbinate tissue biopsy was performed during surgery and was assessed for allergen sIgE (dust mite, grass [temperate or subtropical], and animal epithelium) using an automated immunoassay. Tissue sIgE was assessed among asymptomatic patients and those with nasal symptoms. Data were presented as median (interquartile range). A receiver operating curve was used to predict the diagnostic utility of turbinate tissue sIgE in determining allergic rhinitis. RESULTS: A total of 160 patients (41.89 ± 14.65 years, 36.9% females) were included. The median tissue sIgE concentration among the asymptomatic nonatopic group of patients was 0.09 (0.08-0.10) kUA/L and tissue sIgE > 0.10 kUA/L was determined as a positive threshold. Inferior turbinate tissue sIgE was shown to be a predictive test for allergic rhinitis (area under curve: 0.87, 95% confidence interval: 0.84-0.90) with 90% sensitivity and 89% negative predictive value. CONCLUSION: Inferior turbinate tissue biopsy sIgE is a sensitive tool to predict allergic rhinitis. The threshold value of 0.1 kUA/L corresponded well with the asymptomatic nonatopic group of patients. This method detects sIgE in the nasal mucosa and may be a useful test for allergic rhinitis in future research.


Assuntos
Imunoglobulina E/análise , Rinite Alérgica/diagnóstico , Conchas Nasais/imunologia , Adulto , Alérgenos/imunologia , Estudos Transversais , Feminino , Humanos , Imunoglobulina E/imunologia , Masculino , Pessoa de Meia-Idade , Mucosa Nasal/imunologia , Curva ROC , Rinite/diagnóstico , Testes Cutâneos , Conchas Nasais/patologia , Conchas Nasais/cirurgia
13.
Med Sci Monit ; 25: 333-340, 2019 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-30633736

RESUMO

BACKGROUND The aim of this study was to determine the efficacy of sublingual administration of Dermatophagoides farinae drops for the treatment of allergic rhinitis (AR) accompanied by adenoid hypertrophy and the effect on immune function in children. MATERIAL AND METHODS Eosinophil counts in peripheral blood before and after treatment were determined; serum levels of immunoglobulin E (IgE), total IgE (T-IgE), immunoglobulin G4 (IgG4), interleukin-2 (IL-2), and interleukin-6 (IL-6) before and after treatment were detected by enzyme-linked immunosorbent assay. RESULTS The total effective rate in the study group was significantly higher than that in the control group (P<0.05). In both the study and control groups, symptom scores, medication scores, eosinophil counts in the peripheral blood, and serum levels of IgE, T-IgE, and IL-6 were significantly lower than those before treatment (P<0.05), while the serum levels of IgG4 and IL-2 were significantly higher than those before treatment (P<0.05). After treatment, symptom scores, medication scores, eosinophil counts in the peripheral blood, and serum levels of IgE, T-IgE, and IL-6 in the study group were significantly lower than those in the control group (P<0.05), while the serum levels of IgG4 and IL-2 were significantly higher in the study group than those in the control group (P<0.05). CONCLUSIONS Sublingual administration of D. farinae drops improved the clinical symptoms of pediatric AR caused by Dermatophagoides mites and improved the immune functions in children.


Assuntos
Dermatophagoides farinae/imunologia , Dessensibilização Imunológica/métodos , Rinite Alérgica/terapia , Tonsila Faríngea/efeitos dos fármacos , Tonsila Faríngea/fisiopatologia , Administração Sublingual , Animais , Asma/imunologia , Criança , Pré-Escolar , Eosinófilos/efeitos dos fármacos , Feminino , Humanos , Imunoglobulina E/análise , Imunoglobulina E/sangue , Imunoglobulina G/análise , Imunoglobulina G/sangue , Interleucina-2/análise , Interleucina-2/sangue , Interleucina-6/análise , Interleucina-6/sangue , Masculino , Estudos Retrospectivos , Imunoterapia Sublingual/métodos , Resultado do Tratamento
14.
Rev Mal Respir ; 36(2): 135-141, 2019 Feb.
Artigo em Francês | MEDLINE | ID: mdl-30686558

RESUMO

INTRODUCTION: In tropical Africa, allergies are not well documented. The objective of this work was to evaluate, by two methods, the sensitization to mites in children followed for respiratory allergy. METHODS: Skin prick-test and IgE assay by REAST test with 3 mites: Dermatophagoides pteronyssinus (D. pteronyssinus), Dermatophagoides farinae (D. farinae) and Blomia tropicalis (B. tropicalis) were carried out in children from 3 to 15 years followed up for asthma and/or allergic rhinitis. The positive results of the two tests were compared. RESULTS: Of the 130 (100%) children included, all eligible for the assay, 119 (91.5%) had the prick-test. The mean age and sex ratio (M/F) were 7±1 year, and 1.6. The association of rhinitis and asthma was the most frequent and found in 66 (55.6%). The sensitivity frequencies for the prick-test and assay were respectively 79% versus 36.1% for B. tropicalis, 71.4% versus 33.4% for D. pteronyssinus and 38.7% versus 37.8% for D. farinae. A moderate correlation between mean papule diameter and mean IgE concentration was observed. CONCLUSION: In African tropical environments, dust mite sensitization in children followed for respiratory allergy is frequent, with the order of frequency being: B. tropicalis, D. pteronyssinus, and D. farinae. The prick-test had better sensitivity than the assay for its evaluation.


Assuntos
Dermatophagoides farinae/imunologia , Dermatophagoides pteronyssinus/imunologia , Pyroglyphidae/imunologia , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/epidemiologia , Adolescente , Animais , Antígenos de Dermatophagoides/imunologia , Asma/diagnóstico , Asma/epidemiologia , Asma/etiologia , Benin/epidemiologia , Criança , Pré-Escolar , Feminino , Humanos , Imunoglobulina E/análise , Masculino , Prevalência , Hipersensibilidade Respiratória/imunologia , Rinite Alérgica/diagnóstico , Rinite Alérgica/epidemiologia , Rinite Alérgica/genética , Testes Cutâneos , Clima Tropical
15.
PLoS One ; 14(1): e0209860, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30608947

RESUMO

Immuno-PCR (iPCR) is one of the methods used for the detection of a wide range of analytes and features the high sensitivity of the polymerase chain reaction (PCR) method. iPCR uses antibodies coupled to DNA, followed by the amplification of the attached DNA using RT-PCR. Two major types of antibody-DNA conjugates are currently used, which are obtained as a result of non-covalent (biotin-streptavidin) or covalent interactions. Using a strain-promoted azide-alkyne cycloaddition (SPAAC), we synthesized covalent DNA-antibody conjugates, optimized the reaction conditions, and developed an efficient protocol for the purification of conjugates, with which all unreacted antibodies and oligonucleotides are separated. Covalent DNA-antibody conjugates were tested with iPCR assays that were previously developed for the detection of IgE and IgM antibodies with the use of the supramolecular complex of 5'- and 3'-biotinylated DNA and streptavidin. The results show that the modification of antibodies with amino groups did not allow us to obtain monolabeled antibodies or antibodies with a strictly defined number of DNA-labels. The degree of labeling determined by the dyes introduced through the azido group reflects the actual labeling degree statistically. If the average labeling degree for azido groups is 1.1, the conjugates contain 25% mono-labeled antibodies, 50% double-labeled antibodies, and 25% unlabeled ones. The specificity of the monoclonal antibody to human IgE (BE5) changed after conjugation with the oligonucleotide. The sensitivity of iPCR in the detection of IgM antibodies produced against the LeC disaccharide using a covalent conjugate was similar to that of a supramolecular complex of 5'- and 3'-biotinylated DNA and streptavidin, but the new procedure is two steps shorter.


Assuntos
Imunoglobulina E/genética , Imunoglobulina M/genética , Reação em Cadeia da Polimerase/métodos , Animais , Anticorpos Monoclonais/genética , Antígenos/imunologia , Biotina , Biotinilação , DNA/genética , Humanos , Imunoglobulina E/análise , Imunoglobulina M/análise , Camundongos , Oligonucleotídeos/genética , Sensibilidade e Especificidade , Estreptavidina
19.
J. investig. allergol. clin. immunol ; 29(3): 213-221, 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-184087

RESUMO

Background: Alcohol consumption is associated with enhanced TH2 immune responses. Objective: To investigate the frequency of false-positive results in serological tests for allergy in alcoholic patients. Methods: A total of 138 alcoholic patients consecutively admitted to hospital underwent a panel of allergy tests that included serum total IgE, a multiallergen IgE test (UniCAP Phadiatop), and skin prick tests to relevant aeroallergens in the area, which were considered the standard reference for atopy. In selected cases with positive specific IgE (sIgE) to cross-reactive carbohydrate determinants (CCDs) on ImmunoCAP, we determined sIgE to hymenoptera venom components (ADVIA Centaur) and a microarray of 103 allergen components (ISAC).Results: Increased serum total IgE (>170 IU/mL) was observed in 59/110 (54%) of nonatopic (skin prick test-negative) patients. The result of the multiallergen IgE test was positive in 46 nonatopic patients (42%). This finding was closely associated with high serum concentrations of total IgE and sIgE to CCDs. The vast majority of patients with positive CCD-sIgE showed positivity to glycosylated plant and hymenoptera allergen components on ISAC and ADVIA Centaur. Only 1 out of 26 patients with positive sIgE to CCD and hymenoptera venom developed honeybee venom allergy after a median follow-up of 166 months. Correlations between measurements of sIgE to CCD markers on ImmunoCAP, ADVIA Centaur, and ISAC were imperfect. Conclusions: Serological tests for allergy should be interpreted with caution in alcoholic patients, who frequently have increased levels of total IgE and CCD-sIgE and subsequent positivity of sIgE to glycosylated allergen components, irrespective of the method used


Antecedentes: El consumo de alcohol se asocia con respuestas inmunes aumentadas de tipo Th2.Objetivo: Investigar la frecuencia de falsos positivos en los tests serológicos de alergia en alcohólicos. Métodos: En un total de 138 pacientes alcohólicos ingresados en el hospital de forma consecutiva se realizó un panel de pruebas de alergia que incluyó la determinación de IgE sérica total, un test de IgE específica multialergeno (UniCAP Phadiatop) y pruebas cutáneas en prick a una batería de aeroalérgenos relevantes en el área, cuya positividad se consideró la referencia para clasificar a los pacientes como atópicos. En casos seleccionados con positividad de IgE específica (sIgE) frente a carbohidratos con reactividad (CCDs) en el ImmunoCAP, se determinó la sIgE a componentes del veneno de hymenópteros (ADVIA Centaur) y a un microarray de 103 componentes alergénicos (ISAC).Resultados: Se observó un aumento de las concentraciones de IgE sérica total (>170 IU/mL) en 59/110 (54%) de los alcohólicos no atópicos (prick test-negativos). Cuarenta y seis alcohólicos no atópicos (42%) presentaban un test de IgE específica multialérgeno positivo. Este hallazgo estuvo estrechamente asociado con la presencia de concentraciones elevadas de IgE total y de sIgE a CCDs. La gran mayoría de los alcohólicos con positividad de sIgE a CCDs mostraron positividad con componentes moleculares glicosilados de plantas e himenópteros en el ISAC y el ADVIA Centaur. Sólo uno de los 26 pacientes con positividad de sIgE a CCDs e himenópteros desarrolló alergia clínica a picadura de abeja tras un seguimiento mediano de 166 meses. La correlación de las determinaciones de sIgE a marcadores de CCD en ImmunoCAP, ADVIA Centaur e ISAC fue imperfecta. Conclusiones: Los tests serológicos de alergia se deben interpretar con precaución en pacientes alcohólicos, que frecuentemente muestran elevación de IgE total, positividad de sIgE a CCDs y, consecuentemente, positividad de sIgE a componentes alergénicos glicosilados, independientemente del método utilizado


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Hipersensibilidade/diagnóstico , Alcoolismo/complicações , Apresentação Cruzada/imunologia , Imunoglobulina E/análise , Reações Falso-Positivas , Testes Sorológicos/estatística & dados numéricos , Erros de Diagnóstico/estatística & dados numéricos
20.
Pol Merkur Lekarski ; 47(282): 217-220, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31945022

RESUMO

Atopic dermatitis is one of the most common inflammatory dermatoses in children and adults. The disorder is associated with significant worsening of quality of life. Due to the frequently suggested pathogenetic relationship with atopic dermatitis, immunoglobulin E seems to be an adequate indicator of the disease. AIM: The aim of the study was to assess the association between the total serum immunoglobulin E levels and the grade of skin lesions measured with the SCORAD and oSCORAD scales in the population of adult patients suffering from severe atopic dermatitis. MATERIALS AND METHODS: The study was performed in the group of 31 patients suffering from atopic dermatitis since early childhood (17 women and 14 men) in 2017. Total IgE was measured by the fluoroenzymatic immunoassay. The Spearman's rank correlation coefficient was used to determine the power of the relationship between the concentration of total IgE and atopic dermatitis severity. The Kruskal-Wallis and Mann- Whitney's tests were used to compare the distribution of IgE concentration and the distribution of atopic dermatitis grade. RESULTS: The statistically significant correlation between SCORAD/ oSCORAD and the measured IgE levels was found in the patients with the IgE level above 3500 IU/ml (19 subjects). CONCLUSIONS: The present study demonstrated that in the patients with severe extrinsic atopic dermatitis, the concentration of total IgE was correlated with the severity of the disease. This suggests an opportunity of employing IgE as an atopic dermatitis biomarker.


Assuntos
Dermatite Atópica , Imunoglobulina E , Adulto , Biomarcadores , Criança , Pré-Escolar , Dermatite Atópica/diagnóstico , Feminino , Humanos , Imunoglobulina E/análise , Masculino , Qualidade de Vida , Índice de Gravidade de Doença
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