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1.
J Immunother Cancer ; 10(1)2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-35091456

RESUMO

INTRODUCTION: Immune checkpoint inhibitors (ICI) are a novel cancer therapeutic that have been successful in treating advanced malignancies; however, they also cause immune-related adverse events (irAE). Given that some irAE are clinically similar to traditional autoimmune diseases, autoantibodies have been suggested as possible biomarkers of irAE. However, there are very little data on autoantibody investigation prior to ICI. Our aim was to determine if specific baseline autoantibodies were associated with irAE and see if changes in autoantibody concentration corresponded with irAE development. METHODS: This study used data from an oncologic clinical trial of adaptive dosing combination ICI therapy in patients with advanced melanoma. Plasma was collected at baseline and 6 weeks after ICI initiation and tested in a microarray of 120 autoantigens commonly associated with autoimmune disease, as well as antinuclear antibody (ANA), rheumatoid factor (RF), and anti-cyclic citrullinated peptide antibody (anti-CCP). Autoantibody concentrations were compared between patients experiencing an organ-specific event versus not. Heatmaps, volcano plots and hierarchical clustering were used to determine autoantibody concentration differences among irAE patient clusters as defined by signal intensity of autoantibodies. Kaplan-Meier curves were created and a log-rank test was performed to assess differences in survival. RESULTS: The microarray analysis demonstrated that patients who experienced specific irAE had fewer differentially expressed autoantibodies at baseline than those that did not have those specific irAE, and a greater fold change (FC) in antibody concentration from baseline to 6 weeks corresponded with specific irAE development. However, no autoantibodies were identified as being predictive of specific events. Time to first irAE was less than 6 weeks in 69% of patients, and these patients had less autoantibodies at baseline. Considering ANA, RF and CCP autoantibodies, there were no significant differences between the seropositive and seronegative patients in irAE development, severity, timing or survival. CONCLUSION: Patients with low autoantibody concentrations at baseline as well as a greater FC in autoantibody concentration over 6 weeks developed more distinct organ-specific irAE. This may suggest differences in the balance of cellular immunity and humoral pathways that are relevant in the pathogenesis of irAE, though further investigation is needed.


Assuntos
Autoanticorpos/sangue , Inibidores de Checkpoint Imunológico/efeitos adversos , Melanoma/tratamento farmacológico , Idoso , Anticorpos Anti-Proteína Citrulinada/sangue , Anticorpos Antinucleares/sangue , Feminino , Humanos , Imunoglobulinas/sangue , Masculino , Melanoma/imunologia , Pessoa de Meia-Idade , Fator Reumatoide/sangue
2.
J Nutr Biochem ; 101: 108947, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35017006

RESUMO

In this study, cyclophosphamide (Cy) was used to treat mice to establish an immunosuppressant model in mice, and the regulatory effects of polysaccharides from Fuzhuan brick tea (FBTPSs) including crude FBTPSs (CFBTPSs) and the purified fraction (FBTPSs-3) on the immune function and gut microbiota of mice were investigated. The results showed that CFBTPSs and FBTPSs-3 restored the levels of body weight, feed intake, immune organ index, cytokine and immunoglobulin A in mice. The Cy-induced injury of gut including intestinal morphology and expression of tight junction proteins were also restored. Furthermore, CFBTPSs and FBTPSs-3 could significantly modulate gut microbiota by increasing the relative abundance of Muribaculaceae and reduceing the relative abundances of Lachnospiraceae, Helicobacteraceae, Clostridaceae, Desulfovibrionaceae and Deferribacteraceae. Moreover, the gut microbiota derived short-chain fatty acids might play an important role in improvement of immune function by FBTPSs. Our results showed that FBTPSs could regulate the immune function of mice, which provided evidences for the development of FBTPSs as potentially functional foods to improve human health.


Assuntos
Microbioma Gastrointestinal , Sistema Imunitário/fisiologia , Polissacarídeos/administração & dosagem , Chá , Animais , Bactérias/classificação , Bactérias/isolamento & purificação , Bactérias/metabolismo , Peso Corporal , Ciclofosfamida , Citocinas/biossíntese , Ingestão de Alimentos , Ácidos Graxos Voláteis/metabolismo , Alimento Funcional , Imunoglobulinas/sangue , Imunossupressores , Intestinos/metabolismo , Intestinos/microbiologia , Intestinos/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Proteínas de Junções Íntimas/metabolismo
3.
Toxicology ; 465: 153047, 2022 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-34838595

RESUMO

Recent evidences suggest the role of chronic lead (Pb) exposure in altering immunological parameters. Present study aimed to systematically review existing literature and synthesize quantitative evidence on the association between chronic Pb exposure and changes in immunological markers. Observational studies reporting immunological markers such as leukocyte derivative counts (CD3+, CD4+, CD8+, CD45+, CD56+, lymphocyte, and total leukocyte), cytokine, Immunoglobulin (Igs), C-reactive protein (CRP) among Pb-exposed and unexposed controls were systematically searched from PubMed, Scopus and Embase digital databases from inception to January 2021. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were adhered during systematic review. Mean differences in the immunological markers between Pb-exposed and control groups were pooled using random-effects model. The heterogeneity was assessed using Cochran-Q test and I2 statistic. The review included forty studies reporting immunological markers in Pb-exposed and unexposed control groups. The occupational Pb-exposed group exhibited significantly higher BLL, impaired immunological markers, characterized by a marginal lowering in lymphocyte count, lymphocyte subsets (CD3+, CD4+, CD4+/CD8+ ratio), IFN-γ and IgG levels, while CD8+, IgM, IgA, IgE, and cytokines (IL-4, IL-6, IL-10, and TNF-α) exhibited a trend of higher values in comparison to the control group. Further, inflammatory marker viz., total leukocyte count was significantly higher among Pb-exposed. The included studies exhibited high levels of heterogeneity. In conclusion, Occupational Pb exposure alters the immunological markers such as the circulating cytokines and leukocyte counts. However, high-quality, multicentered studies are required to strengthen present observations and further understand the Pb's role on the immune system. Prospero Registration ID: CRD42021228252.


Assuntos
Poluentes Ambientais/efeitos adversos , Sistema Imunitário/efeitos dos fármacos , Chumbo/efeitos adversos , Subpopulações de Linfócitos/efeitos dos fármacos , Exposição Ocupacional/efeitos adversos , Saúde do Trabalhador , Antígenos CD/sangue , Biomarcadores/sangue , Citocinas/sangue , Humanos , Sistema Imunitário/imunologia , Sistema Imunitário/metabolismo , Imunoglobulinas/sangue , Mediadores da Inflamação/sangue , Subpopulações de Linfócitos/imunologia , Subpopulações de Linfócitos/metabolismo , Medição de Risco
4.
Toxicology ; 465: 153057, 2022 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-34864091

RESUMO

The large conjugated π bond in the molecular structure of carbon nanotubes (CNTs) interacts with the benzene ring structure in di (n-butyl) phthalates (DBP) through a π - π bond. Compounds of CNTs and DBP form easily, becoming another environmental pollutant of concern. We explore whether CNTs entering animals slow down the degradation of the DBP adsorbed in the CNT cavity, thereby prolonging the "hormonal activity" of DBP. In our study, male BALb/c mice were used as experimental subjects divided into four groups: the control group; the multi-walled carbon nanotubes (MWCNTs) exposure group (10mg/kg/d); the DBP exposure group (2.15 mg/kg/d); and the compound exposure group (MWCNTs + DBP). After 30 days of exposure, the mice were sacrificed and their spleens used for immunotoxicology study. The results showed that the exposure groups exhibited splenomegaly and suffered severe oxidative damage to the spleen. In the compound exposure group: levels of IgA and IgG in the serum of the mice changed, and were significantly different from levels in both the MWCNTs and DBP exposure groups (p <0.05); the pathological sections of the spleen showed that the boundary between the white pulp area (WP) and the red pulp area (RP) was blurred, that the cell arrangement was loose, and that more red blood cells were retained in the spleen. Proteomics mass spectrometry analysis showed that compared with the control group, 70 proteins were up-regulated and 27 proteins were down-regulated in the MWCNTs group, 36 proteins were up-regulated and 23 proteins were down-regulated in the DBP group, 87 proteins were up-regulated and 21 proteins were down-regulated in the compound exposure group. The results of GO enrichment analysis and KEGG enrichment analysis of the differentially expressed proteins showed that the compound exposure harmed the spleen antigen recognition, processing, and presentation, inhibited the activation and proliferation of B cells and T cells, and hindered the adaptive immune responses. Our results showed that MWCNTs and DBP compounds can damage the spleen, and impair the innate and adaptive immune functions of the body.


Assuntos
Dibutilftalato/toxicidade , Poluentes Ambientais/toxicidade , Nanotubos de Carbono/toxicidade , Baço/efeitos dos fármacos , Esplenomegalia/induzido quimicamente , Imunidade Adaptativa/efeitos dos fármacos , Animais , Redes Reguladoras de Genes , Imunidade Inata/efeitos dos fármacos , Imunoglobulinas/sangue , Masculino , Camundongos Endogâmicos BALB C , Estresse Oxidativo/efeitos dos fármacos , Proteoma/efeitos dos fármacos , Proteoma/metabolismo , Medição de Risco , Baço/imunologia , Baço/metabolismo , Baço/patologia , Esplenomegalia/imunologia , Esplenomegalia/metabolismo , Esplenomegalia/patologia , Transcriptoma/efeitos dos fármacos
5.
Gastroenterol Hepatol ; 45(1): 47-58, 2022 Jan.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-34029644

RESUMO

INTRODUCTION: Correctly identifying patients with biliary atresia (BA), while avoiding invasive diagnostic methods is challenging. The purpose of this study was to determine the value of serum immune indicators for distinguishing BA from other causes of cholestasis in infants. PATIENTS AND METHODS: The data of infants with a surgical/histological diagnosis of BA and those with other causes of cholestatic jaundice were retrospectively analyzed. Patients were divided into a BA group and a cholestasis control (CC) group. Biochemical parameters, major lymphocyte subsets, immunoglobin and C3 and C4 complement levels were compared between the groups. RESULTS: A total of 129 infants with BA and 63 with other causes of cholestasis (CC control group) with a median age of 2.2 months were included in the analysis. The levels of CD3+ T cells, CD3+CD4+ T cells, and premature T cells and the levels of C3 and C4 were all significantly higher in the BA group compared to the CC group (all P<0.05). Pair-wise correlation analyses indicated that C3 and C4 had a significant positive correlation with γ-GT in the BA group, but not in the CC group. Five indices were found to be significantly associated with BA: stool color, globulin, γ-GT, C3 and C4. A model incorporating stool color, gamma-glutamyl transpeptidase level, and C3 level exhibited an area under the ROC curve (AUC) of 0.93, and a sensitivity of 93% and specificity of 83% for the diagnosis of BA. CONCLUSIONS: Models incorporating serum C3 levels may be useful for accurately diagnosing BA in infants.


Assuntos
Atresia Biliar/sangue , Atresia Biliar/diagnóstico , Complemento C3/análise , Área Sob a Curva , Atresia Biliar/complicações , Complemento C4/análise , Feminino , Humanos , Imunoglobulinas/sangue , Lactente , Icterícia Obstrutiva/etiologia , Subpopulações de Linfócitos , Masculino , Estudos Retrospectivos , Sensibilidade e Especificidade , gama-Glutamiltransferase/sangue
6.
Clin Immunol ; 235: 108791, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34214649

RESUMO

Coronavirus Disease 2019 (COVID-19) emerged as a global pandemic resulting in significant mortality and morbidity. COVID-19 vaccines have been shown to be highly effective in preventing COVID-19 infections and significantly reducing disease severity and mortality. We report on a novel COVID-19 antibody assay using a unique platform to rapidly detect SARS-CoV-2 antibodies with a drop of fingerstick blood in a subject following COVID-19 vaccination. We show early detection of SARS-CoV-2 antibodies post vaccination and persistence of detectable antibodies for at least 6 months. Rapid point of care COVID-19 antibody tests might have a role in assessing the appearance and durability of immune response following COVID-19 vaccination.


Assuntos
Anticorpos Antivirais/imunologia , Coleta de Amostras Sanguíneas/métodos , COVID-19/imunologia , Imunoglobulinas/imunologia , SARS-CoV-2/imunologia , Anticorpos Antivirais/sangue , COVID-19/epidemiologia , COVID-19/virologia , Teste Sorológico para COVID-19/métodos , Dedos , Humanos , Imunoglobulinas/sangue , Masculino , Pessoa de Meia-Idade , Pandemias/prevenção & controle , Reprodutibilidade dos Testes , SARS-CoV-2/fisiologia , Vacinação
7.
J Allergy Clin Immunol ; 149(1): 315-326.e9, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34146579

RESUMO

BACKGROUND: Noninfectious complications are the greatest cause of morbidity and mortality in common variable immunodeficiency (CVID), but their pathogenesis remains poorly defined. OBJECTIVE: Using high-throughput approaches, we aimed to identify, correlate, and determine the significance of immunologic features of CVID with noninfectious complications (CVIDc). METHODS: We simultaneously applied proteomics, RNA sequencing, and mass cytometry to a large cohort with primary antibody deficiency. RESULTS: CVIDc is differentiated from uncomplicated CVID, other forms of primary antibody deficiency, and healthy controls by a distinct plasma proteomic profile. In addition to confirming previously reported elevations of 4-1BB, IL-6, IL-18, and IFN-γ, we found elevations of colony-stimulating factor 1, IL-12p40, IL-18R, oncostatin M, TNF, and vascular endothelial growth factor A to differentiate CVIDc. This cytokine dysregulation correlated with deficiency of LPS-specific antibodies and increased soluble CD14, suggesting microbial translocation. Indicating potential significance of reduced LPS-specific antibodies and resultant microbial-induced inflammation, CVIDc had altered LPS-induced gene expression matching plasma proteomics and corresponding with increased CD14+CD16- monocytes, memory T cells, and tissue inflammation ameliorated by T-cell-targeted therapy. Unsupervised machine learning accurately differentiated subjects with CVIDc and supported cytokine dysregulation, antibody deficit, and T-cell activation as defining and convergent features. CONCLUSIONS: Our data expand understanding of CVIDc proteomics, establish its link with deficiency of IgA and LPS-specific antibodies, and implicate altered LPS-induced gene expression and elevated monocytes and T cells in this cytokine dysregulation. This work indicates that CVIDc results when insufficient antibody neutralization of pathogen-associated molecular patterns, like LPS, occurs in those with a heightened response to these inflammatory mediators, suggesting a 2-hit model of pathogenesis requiring further exploration.


Assuntos
Imunodeficiência de Variável Comum/imunologia , Citocinas/imunologia , Imunoglobulinas/deficiência , Adulto , Células Cultivadas , Imunodeficiência de Variável Comum/sangue , Feminino , Expressão Gênica , Humanos , Imunoglobulinas/sangue , Leucócitos Mononucleares/imunologia , Receptores de Lipopolissacarídeos/sangue , Lipopolissacarídeos/imunologia , Masculino , Pessoa de Meia-Idade
8.
Behav Brain Res ; 416: 113532, 2022 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-34416302

RESUMO

Predation risk can program offspring behavior, physiology, and fitness through maternal effect, but most studies have mainly focused on this effect during pregnancy; little is known about the effect of postpartum predation risk on offspring's phenotype. Here, we compared the antipredator behaviors of adult offspring (approximately 90 days old) produced by female Brandt's voles (Lasiopodomys brandtii) exposed to one of three treatments: cat odor (CO), rabbit odor (RO), and distilled water (DW) for 60 min daily from postpartum day 1-18. Basal levels of plasma adrenocorticotropic hormone (ACTH) and corticosterone (CORT), hypothalamic corticotrophin releasing hormone (CRH), as well as spleen immunoglobulins (IgA, IgM, and IgG) were also measured. Our data showed that the offspring of CO-exposed mothers displayed less head-out behavior to acute 15-min CO exposure, and female offspring showed more freezing behavior. CO offspring showed significantly lower basal ACTH and CORT levels than the RO and DW offspring. Additionally, female but not male CO offspring had higher hypothalamic CRH expression and spleen IgG levels than controls, showing a sex-specific effect. These findings demonstrate that postpartum maternal predator risk exposure promotes a passive-avoidant response to these cues in adult offspring, showing a cross-generational maternal effect of postpartum predation risk. Further, these changes may be associated with alterations in the hypothalamic-pituitary-adrenal axis and immune function.


Assuntos
Arvicolinae , Imunoglobulinas/sangue , Exposição Materna , Odorantes , Período Pós-Parto/imunologia , Comportamento Predatório/fisiologia , Hormônio Adrenocorticotrópico/sangue , Animais , Arvicolinae/imunologia , Arvicolinae/fisiologia , Corticosterona/sangue , Corticosterona/fisiologia , Hormônio Liberador da Corticotropina/metabolismo , Feminino , Sistema Hipotálamo-Hipofisário/metabolismo , Masculino , Sistema Hipófise-Suprarrenal/metabolismo
9.
Cell Mol Biol (Noisy-le-grand) ; 67(3): 11-23, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34933737

RESUMO

The use of plant extracts represents a promising approach for the synthesis of silver nanoparticles (AgNPs). This study reports the low-cost, green synthesis of AgNPs using the extract of clove and black seeds. The biosynthesized AgNPs were confirmed and characterized by analysis of the spectroscopy profile of the UV-visible spectrophotometer. The purpose of the present study is to evaluate the inhibitory effect concentration (MIC) of AgNPs, clove, and black cumin seed extracts on the growth and swarming of P. mirabilis. Clinical isolates of P. mirabilis were isolated from patients suffering from urinary tract infections. Thirteen types of antibiotics were used in the present study to detect their ability to inhibit P. mirabilis's resistance. Immunological findings included the determination of serum levels of IgG, IgM, IgA and complement protein C3 and C4. Results showed that IgG and IgA concentrations significantly increased (1311.13 ± 72.54 and 279 ± 21.31) respectively in UTI patients in comparison to the healthy control group which was 1089.88 ± 37.33 and 117.611 ± 4.19 respectively, While IgM concentrations were increased non significantly in UTI patients (153.331 ± 6.45) in comparison to healthy control (145.2 ± 13.49). Complement components C3 showed a significant increase in UTI patients with mean values of 125.95 ± 6.22 compared to the control group with mean values of 55.191 ± 9.64, while C4 showed statically non-significant among UTI patients in comparison with the control group (35.195 ± 2.34 and 34.371 ± 1.22) respectively.


Assuntos
Proteínas do Sistema Complemento/metabolismo , Imunoglobulinas/sangue , Nanopartículas Metálicas/administração & dosagem , Extratos Vegetais/farmacologia , Proteus mirabilis/efeitos dos fármacos , Prata/administração & dosagem , Infecções Urinárias/sangue , Antibacterianos/administração & dosagem , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/administração & dosagem , Antioxidantes/farmacologia , Biofilmes/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Humanos , Nanopartículas Metálicas/química , Testes de Sensibilidade Microbiana , Nigella sativa/química , Extratos Vegetais/administração & dosagem , Proteus mirabilis/genética , Proteus mirabilis/fisiologia , Prata/química , Espectrofotometria/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Syzygium/química , Infecções Urinárias/metabolismo , Infecções Urinárias/microbiologia
10.
Anal Chem ; 93(46): 15236-15242, 2021 11 23.
Artigo em Inglês | MEDLINE | ID: mdl-34762405

RESUMO

In multiple myeloma (MM) disease, malignant plasma cells produce excessive quantities of a monoclonal immunoglobulin (Ig), known as M-protein. M-protein levels are measured in the serum of patients with MM using electrophoresis techniques to determine the response to treatment. However, therapeutic monoclonal antibodies, such as isatuximab, may confound signals using electrophoresis assays. We developed a robust assay based on immunocapture and liquid chromatography coupled to high-resolution mass spectrometry (IC-HPLC-HRMS) in order to eliminate this interference. Following immunocapture of Ig and free light chains (LC) in serum, heavy chains (HC) and LC were dissociated using dithiothreitol, sorted by liquid chromatography and analyzed using HRMS (Q-Orbitrap). This method allowed the M-proteins to be characterized and the signals from isatuximab and M-proteins to be discriminated. As M-protein is specific to each patient, no standards were available for absolute quantification. We therefore used alemtuzumab (an IgG kappa mAb) as a surrogate analyte for the semiquantification of M-protein in serum. This assay was successfully validated in terms of selectivity/specificity, accuracy/precision, robustness, dilution linearity, and matrix variability from 10.0 to 200 µg/mL in human serum. This method was used for clinical assessment of samples and eliminated potential interference due to isatuximab when monitoring patients with MM.


Assuntos
Anticorpos Monoclonais , Imunoglobulinas/sangue , Mieloma Múltiplo/diagnóstico , Anticorpos Monoclonais Humanizados , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Humanos , Espectrometria de Massas
11.
Front Immunol ; 12: 738123, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34650561

RESUMO

The diversity of B cell subsets and their contribution to vaccine-induced immunity in humans are not well elucidated but hold important implications for rational vaccine design. Prior studies demonstrate that B cell subsets distinguished by immunoglobulin (Ig) isotype expression exhibit divergent activation-induced fates. Here, the antigen-specific B cell response to tetanus toxoid (TTd) booster vaccination was examined in healthy adults, using a dual-TTd tetramer staining flow cytometry protocol. Unsupervised analyses of the data revealed that prior to vaccination, IgM-expressing CD27+ B cells accounted for the majority of TTd-binding B cells. 7 days following vaccination, there was an acute expansion of TTd-binding plasmablasts (PB) predominantly expressing IgG, and a minority expressing IgA or IgM. Frequencies of all PB subsets returned to baseline at days 14 and 21. TTd-binding IgG+ and IgA+ memory B cells (MBC) exhibited a steady and delayed maximal expansion compared to PB, peaking in frequencies at day 14. In contrast, the number of TTd-binding IgM+IgD+CD27+ B cells and IgM-only CD27+ B cells remain unchanged following vaccination. To examine TTd-binding capacity of IgG+ MBC and IgM+IgD+CD27+ B cells, surface TTd-tetramer was normalised to expression of the B cell receptor-associated CD79b subunit. CD79b-normalised TTd binding increased in IgG+ MBC, but remained unchanged in IgM+IgD+CD27+ B cells, and correlated with the functional affinity index of plasma TTd-specific IgG antibodies, following vaccination. Finally, frequencies of activated (PD-1+ICOS+) circulating follicular helper T cells (cTFH), particularly of the CXCR3-CCR6- cTFH2 cell phenotype, at their peak expansion, strongly predicted antigen-binding capacity of IgG+ MBC. These data highlight the phenotypic and functional diversity of the B cell memory compartment, in their temporal kinetics, antigen-binding capacities and association with cTFH cells, and are important parameters for consideration in assessing vaccine-induced immune responses.


Assuntos
Vacina contra Difteria e Tétano/administração & dosagem , Imunização Secundária , Imunoglobulinas/sangue , Memória Imunológica/efeitos dos fármacos , Toxina Tetânica/administração & dosagem , Antígenos CD79/metabolismo , Vacina contra Difteria e Tétano/efeitos adversos , Vacina contra Difteria e Tétano/imunologia , Voluntários Saudáveis , Humanos , Proteína Coestimuladora de Linfócitos T Induzíveis/metabolismo , /metabolismo , Fenótipo , Receptor de Morte Celular Programada 1/metabolismo , Células T Auxiliares Foliculares/imunologia , Células T Auxiliares Foliculares/metabolismo , Toxina Tetânica/efeitos adversos , Toxina Tetânica/imunologia , Membro 7 da Superfamília de Receptores de Fatores de Necrose Tumoral/metabolismo
12.
PLoS One ; 16(10): e0258500, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34644359

RESUMO

Chlamydial infections, caused by a group of obligate, intracellular, gram-negative bacteria, have health implications for animals and humans. Due to their highly infectious nature and zoonotic potential, staff at wildlife rehabilitation centers should be educated on the clinical manifestations, prevalence, and risk factors associated with Chlamydia spp. infections in raptors. The objectives of this study were to document the prevalence of chlamydial DNA shedding and anti-chlamydial antibodies in raptors admitted to five wildlife rehabilitation centers in California over a one-year period. Chlamydial prevalence was estimated in raptors for each center and potential risk factors associated with infection were evaluated, including location, species, season, and age class. Plasma samples and conjunctiva/choana/cloaca swabs were collected for serology and qPCR from a subset of 263 birds of prey, representing 18 species. Serologic assays identified both anti-C. buteonis IgM and anti-chlamydial IgY antibodies. Chlamydial DNA and anti-chlamydial antibodies were detected in 4.18% (11/263) and 3.14% (6/191) of patients, respectively. Chamydial DNA was identified in raptors from the families Accipitridae and Strigidae while anti-C.buteonis IgM was identified in birds identified in Accipitridae, Falconidae, Strigidae, and Cathartidae. Two of the chlamydial DNA positive birds (one Swainson's hawk (Buteo swainsoni) and one red-tailed hawk (Buteo jamaicensis)) were necropsied, and tissues were collected for culture. Sequencing of the cultured elementary bodies revealed a chlamydial DNA sequence with 99.97% average nucleotide identity to the recently described Chlamydia buteonis. Spatial clusters of seropositive raptors and raptors positive for chlamydial DNA were detected in northern California. Infections were most prevalent during the winter season. Furthermore, while the proportion of raptors testing positive for chlamydial DNA was similar across age classes, seroprevalence was highest in adults. This study questions the current knowledge on C. buteonis host range and highlights the importance of further studies to evaluate the diversity and epidemiology of Chlamydia spp. infecting raptor populations.


Assuntos
Doenças das Aves/epidemiologia , Infecções por Chlamydia/epidemiologia , Chlamydia/isolamento & purificação , Aves Predatórias/microbiologia , Animais , Animais Selvagens , Anticorpos Antibacterianos/sangue , Doenças das Aves/imunologia , Doenças das Aves/microbiologia , California/epidemiologia , Chlamydia/classificação , Chlamydia/genética , Chlamydia/imunologia , Infecções por Chlamydia/imunologia , Infecções por Chlamydia/microbiologia , Cloaca/microbiologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Bacteriano/metabolismo , Imunoglobulina M/sangue , Imunoglobulinas/sangue , Filogenia , Prevalência , Centros de Reabilitação , Fatores de Risco , Análise de Sequência de DNA
13.
Toxicology ; 463: 152970, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34606951

RESUMO

Intrauterine exposure to particulate matter (PM) has been associated with an increased risk of asthma development, which may differ by the age of asthma onset, sex, and pollutant concentration. To investigate the pulmonary effects of in utero exposure to concentrated urban ambient particles (CAPs) in response to house dust mite (HDM) sensitization in juvenile mice. Mice were exposed to CAPs (600 µg/m3 PM2.5) during the gestational period. Twenty-two-day postnatal mice were sensitized with HDM (100 µg, intranasally, 3 times per week). Airway responsiveness (AHR), serum immunoglobulin, and lung inflammation were assessed after 43 days of the postnatal period. Female (n = 47) and male (n = 43) mice were divided into four groups as follows: (1) FA: not exposed to CAPs; (2) CAPs: exposed to CAPs; (3) HDM: sensitized to HDM; and (4) CAPs+HDM: exposed to CAPs and HDM-sensitized. PM2.5 exposure did not worsen lung hyperresponsiveness or allergic inflammation in sensitized animals. The levels of the lung cytokines IL-4, TNF-α, and IL-2 were differentially altered in male and female animals. Males presented hyporesponsiveness and increased lung macrophagic inflammation. There were no epigenetic changes in the IL-4 gene. In conclusion, intrauterine exposure ambient PM2.5 did not worsened allergic pulmonary susceptibility but affected the pulmonary immune profile and lung function, which differed by sex.


Assuntos
Pulmão/imunologia , Exposição Materna/efeitos adversos , Material Particulado/toxicidade , Efeitos Tardios da Exposição Pré-Natal/imunologia , Animais , Citocinas/imunologia , Feminino , Imunoglobulinas/sangue , Imunoglobulinas/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Material Particulado/imunologia , Pneumonia/imunologia , Gravidez , Pyroglyphidae/imunologia , Hipersensibilidade Respiratória/imunologia
14.
Ann Hematol ; 100(12): 3007-3016, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34477951

RESUMO

Secondary immunodeficiencies are frequently observed after allo-HSCT. The efficacy of subcutaneous IgG preparations in this population is unknown. A retrospective single-institution study involved 126 adult patients transplanted in 2012-2019 for hematological malignancies. Patients were tested every 2-3 weeks for plasma IgG concentration during the 1st year after transplantation and supplemented with facilitated subcutaneous immunoglobulin when they either had IgG concentration < 500 mg/dl or between 500 and 700 mg/dl and recurrent infection. The IgG concentration < 500 mg/dL was diagnosed in 41 patients, while 500-700 mg/dL in 25 and altogether 53 patients received IgG supplementation. The median number of IgG administrations was 2. The median time to the first IgG administration after allo-HSCT was 4.1 months, while to the next administration (if more than one was required) 53 days (prophylactic group) and 32 days (group with infections). We did not observe any significant toxicity. Two situations were associated with increased probability of meeting criteria for IgG supplementation: diagnosis of either acute lymphoblastic leukemia (ALL) or chronic lymphocytic leukemia (CLL) (83.8% versus 39.3% for other diagnosis, p = 0.000) and the systemic use of corticosteroids (64.2% versus 31.5% for patients without systemic corticosteroids, p = 0.005). Over 40% of the adult recipients may require at least incidental immunoglobulin supplementation during the first year after allo-HSCT. Low IgG concentrations are associated with inferior outcomes. The subcutaneous route of IgG administration appeared to be safe and may allow for long persistence.


Assuntos
Agamaglobulinemia/etiologia , Agamaglobulinemia/terapia , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Imunoglobulinas/uso terapêutico , Administração Cutânea , Adulto , Agamaglobulinemia/sangue , Gerenciamento Clínico , Feminino , Neoplasias Hematológicas/terapia , Humanos , Imunoglobulinas/administração & dosagem , Imunoglobulinas/sangue , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Retrospectivos , Transplante Homólogo/efeitos adversos , Adulto Jovem
15.
Rev. bras. med. esporte ; 27(5): 464-467, July-Sept. 2021. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1288615

RESUMO

ABSTRACT Introduction: Immunity is closely related to health. When the body's immunity is strong, it is healthy. On the contrary, various diseases appear. Sports dance is an entertainment and fitness sports project that integrates sports, music, aesthetics, and dance, the body movement dance as the necessary content and two-person or collective exercises as the primary form of exercise. Studies have shown that long-term adherence to Tai Chi exercise can significantly increase the serum immunoglobulin IgA, IgG, and IgM levels. Objective: The paper explores the effect of physical dance exercise on serum immunoglobulin and T lymphocyte subsets of college students. Methods: The thesis randomly selected 16 male and female students in the first-grade physical dance optional course of public physical education as the experimental group. They performed physical dance exercises three times a week, 40 minutes each time, and the training intensity was controlled at a heart rate of 135-150 beats/min. Ten weeks; besides, 16 male and female students in the first grade were selected as the control group, and no physical dance exercise was performed; all the subjects were drawn from the elbow venous blood on an empty stomach at the same time before and after the experiment to measure serum immunoglobulin and T lymph Cell subpopulation content. Results: After ten weeks of sports dance training, the serum immunoglobulin IgG of both men and women in the experimental group increased significantly (P<0.01), and the CD4+% and CD4+/CD8+ ratio of T lymphocyte subgroups showed extremely significant and significant increases (P <0.01, P<0.05), serum IgM tended to increase, IgA, CD8+% tended to decrease, but there was no significant change. Conclusions: Long-term physical dance exercise can improve the body's immune function. Level of evidence II; Therapeutic studies - investigation of treatment results.


RESUMO Introdução: la inmunidad está estrechamente relacionado con la salud. Cuando la inmunidad del cuerpo es fuerte éste está sano. En caso contrario, aparecen diversas enfermedades. La danza deportiva é um projeto de entretenimiento y fitness que integra el deporte, la música, la estética y la danza, el movimiento corporal como contenido necesario y los ejercicios de das personas ou colectivos como forma principal de ejercicio. Los estudios han demonstraram que a adesão a largo plazo al ejercicio de Tai Chi pode aumentar também os niveles séricos de imunoglobulina IgA, IgG e IgM. Objetivo: O artigo explora o efeito do exercício físico de danza em subconjuntos de imunoglobulina sérica e linfocitos T de estudiantes universitários. Métodos: Para que a tese seja selecionada aleatoriamente, 16 ex-alunos e ex-alunos do curso optativo de danza física de primer grado de educação física pública como grupo experimental. Realizaron ejercicios de danza física tres veces por semana, 40 minutes cada vez, y la intensidad del entrenamiento se controló a una frecuencia cardíaca de 135-150 latidos/min. durante as semanas; además, se selecionar 16 alumnos y alumnas de primer grado como group de control, el que no realizó ningún ejercicio físico de danza. A todos os sujetos se les extrajo sangre venosa del codo com o estómago vacío, durante, antes e depois do experimento para medir o conteúdo da subpoblación de células T y de imunoglobulina sérica. Resultados: Después de diez semanas de entrenamiento de danza deportiva, la imunoglobulina IgG sérica de hombres y mujeres en el grupo experimental aumentó complementar (P <0,01), y la proporción CD4 +% y CD4 +/CD8 + de los subgrupos de linfocitos T mostró incrementos extremadamente provados. (P <0,01,P <0,05), la IgM sérica tendió a aumentar, la IgA, CD8 +% tendió a disminuir, mas nenhum hubo cambio projetado. Conclusiones: El ejercicio de danza física a largo plazo puede mejorar la función inmunológica del cuerpo. Nivel de evidencia II; Estudios terapéuticos: investigación de los results del tratamiento.


RESUMEN Introducción: la inmunidad está estrechamente relacionada con la salud. Cuando la inmunidad del cuerpo es fuerte éste está sano. En caso contrario, aparecen diversas enfermedades. La danza deportiva es un proyecto de entretenimiento y fitness que integra el deporte, la música, la estética y la danza, el movimiento corporal como contenido necesario y los ejercicios de dos personas o colectivos como forma principal de ejercicio. Los estudios han demostrado que la adherencia a largo plazo al ejercicio de Tai Chi puede aumentar significativamente los niveles séricos de inmunoglobulina IgA, IgG e IgM. Objetivo: El artículo explora el efecto del ejercicio físico de danza en subconjuntos de inmunoglobulina sérica y linfocitos T de estudiantes universitarios. Métodos: Para la tesis se seleccionaron aleatoriamente a 16 alumnos y alumnas del curso optativo de danza física de primer grado de educación física pública como grupo experimental. Realizaron ejercicios de danza física tres veces por semana, 40 minutos cada vez, y la intensidad del entrenamiento se controló a una frecuencia cardíaca de 135-150 latidos/min. durante diez semanas; además, se seleccionaron 16 alumnos y alumnas de primer grado como grupo de control, el que no realizó ningún ejercicio físico de danza. A todos los sujetos se les extrajo sangre venosa del codo con el estómago vacío, durante, antes y después del experimento para medir el contenido de la subpoblación de células T y de inmunoglobulina sérica. Resultados: Después de diez semanas de entrenamiento de danza deportiva, la inmunoglobulina IgG sérica de hombres y mujeres en el grupo experimental aumentó significativamente (P <0.01), y la proporción CD4 +% y CD4 + / CD8 + de los subgrupos de linfocitos T mostró incrementos extremadamente significativos. (P <0.01, P <0.05), la IgM sérica tendió a aumentar, la IgA, CD8 +% tendió a disminuir, pero no hubo cambios significativos. Conclusiones: El ejercicio de danza física a largo plazo puede mejorar la función inmunológica del cuerpo. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Universidades , Imunoglobulinas/sangue , Dança/fisiologia , Imunidade/fisiologia , Serviços de Saúde para Estudantes , Fatores de Tempo , Contagem de Linfócitos
16.
Sci Rep ; 11(1): 17015, 2021 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-34426626

RESUMO

Almost 50% of the world's esophageal cancer (EC) cases occur in China, and the impact of cancer screening has long been a controversial topic. The study was designed to evaluate the biological correlates of EC screening and subsequent diagnosis in China. Based on the national cohort of esophageal cancer program, a prospective multicenter study in high-risk regions was conducted from 2017 to 2019. 61 participants received twice esophageal endoscopy screening and pathological biopsy successively (with a mean follow-up of 14.03 months). Box-Cox-power transformation and two-way repeated measures ANOVA were used to evaluate hormone cortisol and immunoglobulin (IgA, IgG, IgM) levels in plasma, reflecting their stress, immune function, and biological correlates before screening and after knowing the diagnosis. The median of cortisol, IgA, IgG, and IgM in pre-screening was 15.46 ug/dL, 1.86 g/L, 12.14 g/L, and 0.91 g/L, corresponding value at post-diagnosis was 15.30 ug/dL, 2.00 g/L, 12.79 g/L, and 0.94 g/L, respectively. No significant differences in biological indicators were found between normal and esophagitis and low-grade intraepithelial neoplasia before screening and after diagnosis. After normality transformation, cortisol, IgA, IgG and IgM levels were (0.25 ± 0.04) U/mL, (0.72 ± 0.13) (g/L), (2.44 ± 0.22) (g/L) and (0.98 ± 0.25) (g/L) before screening, (0.25 ± 0.05) U/mL, (0.70 ± 0.13) (g/L), (2.48 ± 0.21) (g/L) and (1.00 ± 0.25) (g/L) after diagnosis, respectively. Repeated Measures ANOVA showed that the main effects were significant on IgA levels between pre-screening and post-diagnosis (P = 0.019). No interaction effects on biological levels between pre-post screening and esophageal pathology, anxiety states (all P > 0.05). Little biological correlates were found both before screening and after diagnosis. Cortisol and IgA dropped less significantly, while IgM and IgA were increased slightly after diagnosis. Further multi-round longitudinal studies are needed to validate these results.


Assuntos
Detecção Precoce de Câncer , Neoplasias Esofágicas/diagnóstico , Ansiedade/sangue , Neoplasias Esofágicas/sangue , Neoplasias Esofágicas/patologia , Esôfago/patologia , Humanos , Hidrocortisona/sangue , Imunoglobulinas/sangue
17.
Life Sci Alliance ; 4(9)2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34226277

RESUMO

Here, we recorded serum proteome profiles of 33 severe COVID-19 patients admitted to respiratory and intensive care units because of respiratory failure. We received, for most patients, blood samples just after admission and at two more later time points. With the aim to predict treatment outcome, we focused on serum proteins different in abundance between the group of survivors and non-survivors. We observed that a small panel of about a dozen proteins were significantly different in abundance between these two groups. The four structurally and functionally related type-3 cystatins AHSG, FETUB, histidine-rich glycoprotein, and KNG1 were all more abundant in the survivors. The family of inter-α-trypsin inhibitors, ITIH1, ITIH2, ITIH3, and ITIH4, were all found to be differentially abundant in between survivors and non-survivors, whereby ITIH1 and ITIH2 were more abundant in the survivor group and ITIH3 and ITIH4 more abundant in the non-survivors. ITIH1/ITIH2 and ITIH3/ITIH4 also showed opposite trends in protein abundance during disease progression. We defined an optimal panel of nine proteins for mortality risk assessment. The prediction power of this mortality risk panel was evaluated against two recent COVID-19 serum proteomics studies on independent cohorts measured in other laboratories in different countries and observed to perform very well in predicting mortality also in these cohorts. This panel may not be unique for COVID-19 as some of the proteins in the panel have previously been annotated as mortality markers in aging and in other diseases caused by different pathogens, including bacteria.


Assuntos
COVID-19/sangue , COVID-19/mortalidade , Proteoma/metabolismo , Índice de Gravidade de Doença , Idoso , COVID-19/virologia , Estudos de Coortes , Feminino , Hospitalização , Humanos , Imunoglobulinas/sangue , Masculino , SARS-CoV-2/fisiologia , Sobreviventes
18.
Dev Comp Immunol ; 123: 104159, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34081944

RESUMO

IFN-γ plays a key role in T-cell activation and the establishment of the adaptive immune response, which has a potential as a cytokine adjuvant in the context of vaccination. In this study, we evaluated the immune adjuvant effects of two forms of flounder (Paralichthys olivaceus) IFN-γ, including pcDNA3.1-IFN-γ (pcIFN-γ) and recombinant IFN-γ (rIFN-γ), and comparatively analyzed the immune responses of flounder to E. tarda subunit vaccine rOmpV. The results showed that vaccination with rOmpV plus pcIFN-γ or rIFN-γ produced a relative percent survival of 57% and 71%, respectively, which were significantly higher than that of the control groups, rOmpV plus pcN3 (36%) or rHis (40%). Compared with the two control groups, vaccination with rOmpV plus pcIFN-γ or rIFN-γ could induce significantly higher levels of specific serum antibodies and sIg + lymphocytes in peripheral blood, spleen and head kidney, and significantly higher upregulated expressions of CD4-1, CD8α, IgM, MHC Ⅰα, MHC Ⅱα, IL-1ß and TNF-α were also detected in rOmpV plus pcIFN-γ or rIFN-γ vaccinated fish. In addition, compared with pcIFN-γ, rOmpV co-vaccination with rIFN-γ elicited higher levels of sIg + lymphocytes, specific serum antibodies and several immune-related genes expressions in vaccinated flounder. These results demonstrated that rOmpV co-vaccination with rIFN-γ or pcIFN-γ could both boost the immune responses and evoke highly protective effects against E. tarda, indicating that flounder IFN-γ is a promising adjuvant candidate for fish vaccination via an injection administering route.


Assuntos
Vacinas Bacterianas/imunologia , Edwardsiella tarda/fisiologia , Infecções por Enterobacteriaceae/imunologia , Linguado/imunologia , Interferon gama/metabolismo , Imunidade Adaptativa , Adjuvantes Imunológicos , Animais , Anticorpos Antibacterianos/sangue , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Imunoglobulinas/sangue , Mediadores da Inflamação/metabolismo , Interferon gama/genética , Ativação Linfocitária , Transgenes/genética , Vacinação , Vacinas de Subunidades
19.
J Occup Health ; 63(1): e12235, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34089209

RESUMO

OBJECTIVE: To evaluate the immunotoxicity and effects of noise and/or low-concentration carbon monoxide (CO) exposure on immune organs and immune functions in rats. METHODS: Male Wistar rats exposed to 98 dB(A) white noise and/or 100 ppm CO 4 h/d for 30 d were used to determine the pathological changes in the thymus and spleen, and variations in leukocyte counts, inflammatory factors, and immunoglobulin (Ig) concentrations. RESULTS: The boundaries of the cortex and medulla of the thymus were unclear following noise and combined exposure. The pathological changes in spleen after CO and combined exposure included blurred boundaries of red-pulp and white-pulp, disappearance of normal splenic nodules and neutrophil infiltration. After exposure to noise and in combination, leukocyte and lymphocyte counts decreased significantly. After exposure to low-concentration CO and in combination, serum IgM and IgG levels decreased significantly, but the levels of tumor necrosis factor-α and interferon-γ levels increased significantly. Eosinophils and IgA levels decreased significantly following exposure to noise and/or low concentration of CO, while the level of interleukin-1 increased significantly. Monocytes increased significantly only under noise or CO exposure, but not under combined exposure. CONCLUSIONS: Noise and/or low-concentration CO exposure may suppress innate and adaptive immune functions and induce inflammatory responses. Noise exposure mainly affected the innate immune function of rats, whereas low-concentration CO exposure mainly affected adaptive immune functions. Combined exposure presented higher immunotoxicity than noise or CO alone, suggesting that exposure to noise and low-concentration CO in the living and working environments can affect the immune system.


Assuntos
Monóxido de Carbono/toxicidade , Exposição Ambiental/efeitos adversos , Imunidade , Imunotoxinas/toxicidade , Ruído/efeitos adversos , Imunidade Adaptativa , Animais , Imunidade Inata , Imunoglobulinas/sangue , Mediadores da Inflamação/sangue , Contagem de Leucócitos , Masculino , Ratos , Ratos Wistar , Baço/patologia , Timo/patologia
20.
Am J Physiol Regul Integr Comp Physiol ; 321(2): R125-R138, 2021 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-34105357

RESUMO

The pregnant Dahl salt-sensitive (S) rat is an established preclinical model of superimposed spontaneous preeclampsia characterized by exacerbated hypertension, increased urinary protein excretion, and increased fetal demise. Because of the underlying immune system dysfunction present in preeclamptic pregnancies in humans, we hypothesized that the pregnant Dahl S rat would also have an altered immune status. Immune system activation was assessed during late pregnancy in the Dahl S model and compared with healthy pregnant Sprague-Dawley (SD) rats subjected to either a sham procedure or a procedure to reduce uterine perfusion pressure (RUPP). Circulating immunoglobulin and cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA) and Milliplex bead assay, respectively, and percentages of circulating, splenic, and placental immune cells were determined using flow cytometry. The pregnant Dahl S rat exhibited an increase in CD4+ T cells, and specifically TNFα+CD4+ T cells, in the spleen compared with virgin Dahl S rats. The Dahl also had increased neutrophils and decreased B cells in the peripheral blood as compared with Dahl virgin rats. SD rats that received the RUPP procedure had increases in circulating monocytes and increased IFN-É£+CD4+ splenic T cells. Together these findings suggest that dysregulated T cell activity is an important factor in both the pregnant Dahl S rats and SD rats after the RUPP procedure.


Assuntos
Linfócitos T CD4-Positivos/imunologia , Citocinas/sangue , Imunoglobulinas/sangue , Placenta/imunologia , Pré-Eclâmpsia/imunologia , Baço/imunologia , Animais , Linfócitos B/imunologia , Linfócitos B/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Modelos Animais de Doenças , Feminino , Idade Gestacional , Neutrófilos/imunologia , Neutrófilos/metabolismo , Fenótipo , Placenta/metabolismo , Pré-Eclâmpsia/sangue , Gravidez , Ratos Endogâmicos Dahl , Ratos Sprague-Dawley , Baço/metabolismo
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