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1.
J Trop Pediatr ; 67(4)2021 08 27.
Artigo em Inglês | MEDLINE | ID: mdl-34478546

RESUMO

BACKGROUND AND OBJECTIVES: Assessing the co-infections with COVID-19 is crucial to delineate its true clinical impact. Pediatric information in this aspect is limited. Our study aims to analyze the spectrum of co-infections in pediatric COVID-19 patients and determine the clinical as well as laboratory parameters predicting co-infection. METHODOLOGY: In this prospective observational study conducted from June to December 2020 in a single tertiary care institution, data pertaining to demographic, illness and treatment-related variables were analyzed among two subsets of pediatric patients of age 1 month-12 years with RT-PCR-confirmed COVID-19 infection-Group A: those with confirmed co-infection and Group B: moderate to severe disease without co-infection. Among Group A, etiology of co-infection was characterized through relevant microbiological examination within 48 h admission. RESULT: Among our study population, 15.03% and 20.6% had co-infections and moderate to severe disease respectively. Among those with confirmed co-infection, 32.5%, 11.6% and 6.97% recorded blood culture, respiratory secretion and CSF growth, respectively, the picture being dominated by Methicillin resistant and sensitive Staphylococcus aureus. Serum serology demonstrated Scrub typhus infection to be most prevalent. Concurrent respiratory viral infections were seen in 11.6%. Children with co-infection had significantly higher morbidity and need for supportive therapy. Predictors of co-infection were localization of infection, Neutrophil count ≥10×109, age-specific lymphopenia, CRP > 100 mg/dl and hyperferritinemia. CONCLUSION: Co-infections are an important factor prognosticating pediatric COVID infection. Their early detection, prompt and appropriate treatment is of paramount importance.


Assuntos
COVID-19 , Coinfecção , Infecções Estafilocócicas , Criança , Coinfecção/epidemiologia , Humanos , Estudos Prospectivos , SARS-CoV-2 , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/epidemiologia
2.
Nanoscale ; 13(29): 12546-12552, 2021 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-34477613

RESUMO

A metal-organic framework (MOF) material was prepared from 2-aminoterephthalic acid and aluminum chloride with a solvothermal synthesis protocol. The as-prepared MOF material named NH2-MIL-53(Al) emitted a very intensive fluorescent (FL) signal after it was hydrolyzed in alkaline solution for releasing numerous FL ligands NH2-H2BDC. Thus it can be considered as a sensitive FL probe for studying biorecognition events. In this proof-of-principle work, a double-site recognition method was established to quantify Staphylococcus aureus (S. aureus) relying on the alkaline hydrolysis property of the MOF material. In particular, magnetic beads (MBs) modified with pig IgG were adopted for binding S. aureus based on the strong affinity between pig IgG and protein A on the bacterial surface. Meanwhile, MOF NH2-MIL-53(Al)-tagged teicoplanin (TEI) was adopted for tracing the target bacteria. By hydrolyzing the MOF material bound on the MBs to trigger the FL signal, S. aureus can be quantified with a dynamic range of 3.3 × 103-3.3 × 107 CFU mL-1 and a detection limit of 5.3 × 102 CFU mL-1 (3σ). The method can exclude efficiently the interference from other common bacteria. It has been applied to quantify S. aureus in saliva, pomegranate green tea, glucose injection and milk samples with satisfactory results, verifying the application potential for analyzing various types of real samples contaminated with S. aureus.


Assuntos
Estruturas Metalorgânicas , Infecções Estafilocócicas , Animais , Corantes Fluorescentes , Hidrólise , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus , Suínos
3.
Pan Afr Med J ; 39: 84, 2021.
Artigo em Francês | MEDLINE | ID: mdl-34466186

RESUMO

The treatment of acute osteomyelitis is becoming more challenging since the emergence of community-acquired methicillin-resistant Staphylococcus aureus. We collected data on all patients with acute osteomyelitis caused by this germ over a period of 21 years (January 1995-December 2016) and we analyzed the peculiarities of this disorder. Our case series includes 15 children, with an average age of 9 years. All patients had affected lower limb. Local trauma was reported in 8 cases and skin carriage in 4 cases. Acute onset was reported in 12 cases associated with pseudo-paralysis of the affected limb. One patient had Staphylococcus aureus pulmonary infection with signs of septicopyemia. Blood culture was positive in 8 cases. In one case PCR assay for detection of Panton-Valentine leukocidin was performed with positive result. All these patients underwent surgical debridement and received secondarily adapted empirical antibiotic therapy. Outcome was good in 8 cases and poor in the other cases, with transition to a chronic state in 6 cases and one case of death. Pathological fracture was reported in 3 cases. Osteomyelitis cause by community-acquired methicillin-resistant Staphylococcus aureus is associated with a pejorative outcome. Recognizing the clinical and paraclinical signs of these infections is essential for a specific and early therapeutic management.


Assuntos
Infecções Comunitárias Adquiridas/diagnóstico , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Osteomielite/diagnóstico , Infecções Estafilocócicas/diagnóstico , Doença Aguda , Adolescente , Antibacterianos/administração & dosagem , Criança , Pré-Escolar , Terapia Combinada , Infecções Comunitárias Adquiridas/microbiologia , Infecções Comunitárias Adquiridas/terapia , Desbridamento/métodos , Feminino , Humanos , Masculino , Osteomielite/microbiologia , Osteomielite/terapia , Estudos Retrospectivos , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/terapia
4.
Cas Lek Cesk ; 160(4): 139-142, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34416816

RESUMO

Staphylococcus aureus (SAB) bacteremia is very serious and often fatal infection with the high incidence and lethality. Diagnosis of SAB must be followed by an appropriate diagnostic and therapeutic process. From the point of view of proper SAB management, it is essential to find the primary source of infection, which can be skin and soft tissue infections, catheter infections, infectious endocarditis, osteomyelitis, pneumonia or abscesses with hematogenous spread. After the SAB has been identified, it is crucial to determine the appropriate examination and treatment procedure in close collaboration with an infectious disease specialist, clinical microbiologist and clinical pharmacist.


Assuntos
Bacteriemia , Infecções Estafilocócicas , Bacteriemia/diagnóstico , Bacteriemia/tratamento farmacológico , Humanos , Incidência , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus
5.
BMJ Case Rep ; 14(8)2021 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-34344645

RESUMO

A 50-year-old man with no medical history of note presented with new onset of confusion and dyspnoea. He tested positive for coronavirus (COVID-19), and subsequently, was admitted to the intensive care unit due to severe sepsis and acute renal failure requiring haemodialysis. Shortly afterwards, he was intubated due to haemodynamic instability. His blood culture was positive for Staphylococcus aureus bacteraemia, and echocardiogram showed evidence of vegetation in the aortic valve area. He was commenced on intravenous antibiotics for infective endocarditis (IE). Following extubation, he underwent an MRI of the spine due to increasing back pain. This was suggestive of L5-S1 discitis, likely secondary to septic emboli from IE. A few days later, he developed acute ischaemia of the left toes and extensive thrombosis of the right cubital and left iliac veins. Following a prolonged hospital admission, he was discharged home and later underwent an elective forefoot amputation from which he made a good recovery.


Assuntos
Bacteriemia , COVID-19 , Endocardite Bacteriana , Endocardite , Infecções Estafilocócicas , Endocardite Bacteriana/complicações , Endocardite Bacteriana/diagnóstico , Endocardite Bacteriana/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , SARS-CoV-2 , Infecções Estafilocócicas/complicações , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus
6.
Front Cell Infect Microbiol ; 11: 634215, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34381737

RESUMO

Bloodstream infections (BSIs), the presence of microorganisms in blood, are potentially serious conditions that can quickly develop into sepsis and life-threatening situations. When assessing proper treatment, rapid diagnosis is the key; besides clinical judgement performed by attending physicians, supporting microbiological tests typically are performed, often requiring microbial isolation and culturing steps, which increases the time required for confirming positive cases of BSI. The additional waiting time forces physicians to prescribe broad-spectrum antibiotics and empirically based treatments, before determining the precise cause of the disease. Thus, alternative and more rapid cultivation-independent methods are needed to improve clinical diagnostics, supporting prompt and accurate treatment and reducing the development of antibiotic resistance. In this study, a culture-independent workflow for pathogen detection and identification in blood samples was developed, using peptide biomarkers and applying bottom-up proteomics analyses, i.e., so-called "proteotyping". To demonstrate the feasibility of detection of blood infectious pathogens, using proteotyping, Escherichia coli and Staphylococcus aureus were included in the study, as the most prominent bacterial causes of bacteremia and sepsis, as well as Candida albicans, one of the most prominent causes of fungemia. Model systems including spiked negative blood samples, as well as positive blood cultures, without further culturing steps, were investigated. Furthermore, an experiment designed to determine the incubation time needed for correct identification of the infectious pathogens in blood cultures was performed. The results for the spiked negative blood samples showed that proteotyping was 100- to 1,000-fold more sensitive, in comparison with the MALDI-TOF MS-based approach. Furthermore, in the analyses of ten positive blood cultures each of E. coli and S. aureus, both the MALDI-TOF MS-based and proteotyping approaches were successful in the identification of E. coli, although only proteotyping could identify S. aureus correctly in all samples. Compared with the MALDI-TOF MS-based approaches, shotgun proteotyping demonstrated higher sensitivity and accuracy, and required significantly shorter incubation time before detection and identification of the correct pathogen could be accomplished.


Assuntos
Bacteriemia , Infecções Estafilocócicas , Bacteriemia/diagnóstico , Candida albicans , Escherichia coli , Humanos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus
7.
Rev Med Liege ; 76(7-8): 595-597, 2021 Jul.
Artigo em Francês | MEDLINE | ID: mdl-34357710

RESUMO

We report a clinical case of a patient hospitalized for community-acquired Staphylococcus aureus pneumonia. A 26-year-old patient with no medical history went to the emergency department for fever. He quickly developed acute respiratory failure and community-acquired Staphylococcus aureus pneumonia as well as bacteremia were confirmed. This pulmonary infection is rare but can affect all age groups and occur in a variety of ways. Patients with community-acquired Staphylococcus aureus pneumonia have more severe clinical outcomes than those with community-acquired pneumonia caused by other germs. The article discusses the main characteristics of community-acquired Staphylococcus aureus pneumonia and recalls the recommendations in case of bacteremia with Staphylococcus aureus.


Assuntos
Infecções Comunitárias Adquiridas , Pneumonia Estafilocócica , Infecções Estafilocócicas , Adulto , Infecções Comunitárias Adquiridas/diagnóstico , Serviço Hospitalar de Emergência , Febre , Humanos , Masculino , Pneumonia Estafilocócica/diagnóstico , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus
8.
Methods Mol Biol ; 2341: 117-125, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34264467

RESUMO

Secreted bacterial proteins are difficult to identify directly from an infection site due to a limited amount of bacteria and presence of a large quantity of host proteins. Here we describe a rat model of orthopedic implant that allows us to harvest bacterial biofilm materials sufficient for identification of bacterial proteins in the biofilm matrix by liquid chromatography-tandem MS (GeLC-MS/MS) analysis.


Assuntos
Proteínas de Bactérias/isolamento & purificação , Biofilmes/crescimento & desenvolvimento , Infecções Relacionadas à Prótese/microbiologia , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus/fisiologia , Animais , Cromatografia Líquida , Modelos Animais de Doenças , Masculino , Próteses e Implantes/microbiologia , Ratos , Staphylococcus aureus/metabolismo , Espectrometria de Massas em Tandem
9.
BMJ Case Rep ; 14(7)2021 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-34301708

RESUMO

Mandibular osteomyelitis in paediatric population presents as painful swelling with progressive trismus. Often the aetiology for this form of progressive osteomyelitis is inconclusive. The infective aetiology in this condition is difficult to assign as bone tissue culture is found to be seldom positive. We present a case of an 11-year-old girl, with hearing and speech impairment, who presented with report of painful right-sided mandibular swelling with progressive trismus. Clinical, radiographic and histopathological findings were suggestive of sclerotic osteomyelitis. Bone tissue culture grew Methicillin-resistant Staphylococcus aureus, indicating a rare infective variant. Unlike the common belief, where osteomyelitic mandible needs a combination of medical and surgical management, our case was managed conservatively with only antibiotic therapy. Our patient responded well to the treatment with reduction in pain and gradual improvement in the mouth opening. Radiographically the mandibular involvement also showed complete regression.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Osteomielite , Infecções Estafilocócicas , Antibacterianos/uso terapêutico , Criança , Feminino , Humanos , Mandíbula/diagnóstico por imagem , Osteomielite/diagnóstico por imagem , Osteomielite/tratamento farmacológico , Infecções Estafilocócicas/complicações , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico
10.
Am J Case Rep ; 22: e930136, 2021 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-34253705

RESUMO

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) pneumonia has well-defined characteristics. We present a case of cavitary pneumonia due to MRSA in a patient who had undergone a recent outpatient gastroscopic procedure. CASE REPORT A 32-year-old man presented at the Emergency Department with tonic-clonic seizures of 2 min durations. He had a history of seizures without current treatment or use of psychostimulant drugs. His personal history referred to hypothyroidism treated with levothyroxine, morbid type 3 obesity, gastritis with a gastric ulcer, penicillin allergies, and an ambulatory endoscopy with a biopsy (7 days ago) for erosive gastropathy. On the 3rd day of admission to the Intensive Care Unit (ICU), a bronchoscopy was performed, which showed a reddened mucosa with hemorrhagic points and a cavitary area in the right main bronchus. Multiple polymerase chain reaction and mass spectrometry analyses of samples of bronchioalveolar lavage from the bronchus revealed MRSA with a mechanism of resistance to the mecA gene (1×105 colony-forming unit/mL). The laboratory results for the cerebrospinal fluid were negative for bacterial growth. CONCLUSIONS This is a rare case of cavitary pneumonia due to MRSA of clinical and epidemiological characteristics, which is unusual after an outpatient endoscopic procedure.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Pneumonia Estafilocócica , Infecções Estafilocócicas , Adulto , Antibacterianos/uso terapêutico , Endoscopia , Humanos , Unidades de Terapia Intensiva , Masculino , Pneumonia Estafilocócica/diagnóstico , Pneumonia Estafilocócica/tratamento farmacológico , Reação em Cadeia da Polimerase , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico
11.
Acta Derm Venereol ; 101(8): adv00515, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34219178

RESUMO

Hand eczema is frequently colonized with Staphylococcus aureus. Some patients with hand eczema wear occlusive gloves regularly; however, the effect of this on the density of S. aureus is unexplored. The aim of this study is to examine the effect of occlusive gloves on the density of S. aureus sampled from the hands of patients with hand eczema. In an experimental set-up, patients with moderate-to-severe hand eczema wore an occlusive glove on one hand for 4 h with a 30-min break. Bacterial swabs were collected from the most severe eczema lesion on the hand before and immediately after glove exposure. S. aureus colony-forming units were counted and log-transformations used for comparison of before- and after-values. Among 30 patients, 19 (63%) were colonized with S. aureus. After glove occlusion S. aureus colony-forming units increased by a factor of 1.72 (p < 0.01). In conclusion, the density of sampled S. aureus on eczematous skin after prolonged wearing of occlusive gloves is greatly increased.


Assuntos
Eczema , Dermatoses da Mão , Infecções Estafilocócicas , Eczema/diagnóstico , Luvas Protetoras , Mãos , Dermatoses da Mão/diagnóstico , Humanos , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus
12.
Clin Lab ; 67(7)2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34258960

RESUMO

BACKGROUND: Staphylococcus aureus is a gram-positive coccus forming grape like clusters performing both aerobic and anaerobic respiration. Most strains of S. aureus ferment mannitol and they form characteristic golden yellow colonies. They produce catalase, coagulase, and extracellular cell clumping factor. Some strains can also produce capsules. It is a major commensal bacterium and a human pathogen that causes a wide range of clinical infections including abscesses of various organs, pneumonia, osteomyelitis, endocarditis, arthritis, and sepsis. S. aureus is the key organism for food poisoning and it is the third most important cause of food borne disorders in the world. METHODS: We reviewed all the relevant literature available on PubMed, Web of Science, and Google Scholar. We selected different scientific studies and reports published in English language which addressed prevalence, pathogenesis, burden and laboratory diagnosis methods of S. aureus to compile the current review. RESULTS: Staphylococcus aureus has an outstanding ability to acquire resistance to most classes of antimicrobial agents. This successful and adaptable resistance has made treatment and control of staphylococcal infections increasingly difficult. Expression of virulence factors of S. aureus is controlled by bacterial cell density and many environmental factors such as pH, oxygen, and carbon dioxide. There are different mechanisms that microorganisms use to prevent attack by antimicrobial agents. These include limiting uptake of the drug, modification of the drug target, inactivation of the drug, and active efflux of the drug. Specimens collected for diagnosis of S. aureus infection depend on the type of infection. The samples for diagnosis are pus, sputum, blood, feces, vomit and the remains of suspected food, and nasal swab for the detection of carriers. Gram stain, culture, biochemical tests, serological tests and molecular techniques are the common laboratory diagnosis methods. CONCLUSIONS AND RECOMMENDATIONS: Multidrug resistant S. aureus strains are emerging and current antibiotics are not efficacious against such strains. Both active and passive immunization strategies have thus far failed to show efficacy in humans. Therefore, infection preventive measures, and further research is required to develop vaccines and antibiotics to target this pathogen.


Assuntos
Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Antibacterianos/uso terapêutico , Coagulase , Atenção à Saúde , Humanos , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/epidemiologia , Staphylococcus aureus
14.
BMJ Case Rep ; 14(6)2021 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-34155010

RESUMO

Septic arthritis of the acromioclavicular joint (ACJ) is a rare pathology with very few documented case reports available in the literature. In addition, ACJ septic arthritis secondary to methicillin-resistant Staphylococcus aureus (MRSA) is a rarer pathology with only two previously documented case reports in the medical literature to our knowledge. We present the case of a 46-year-old man who was diagnosed with a confirmed ACJ septic arthritis secondary to MRSA following admission to the local trauma and orthopaedic service at a district general hospital. We aim to give an insight into this pathology and highlight the importance of the relationship between orthopaedics and microbiology in optimising patient care, particularly in a period of rising rates of antimicrobial resistance.


Assuntos
Articulação Acromioclavicular , Artrite Infecciosa , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Articulação Acromioclavicular/diagnóstico por imagem , Antibacterianos/uso terapêutico , Artrite Infecciosa/diagnóstico , Artrite Infecciosa/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico
15.
BMJ Case Rep ; 14(6)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34162615

RESUMO

A 46-year-old woman with a history of end-stage renal disease on chronic haemodialysis presented with 1 week of fever, chills, altered mental status and hand pain. She was febrile and ill-appearing on presentation with a pulse rate of 102 beats per minute. She had a tunnelled dialysis catheter in her right neck. Hand examination demonstrated a swollen, erythematous and tender wrist. Cardiovascular examination demonstrated no murmurs. CT of the hand showed abscesses involving the left forearm. Blood and abscess cultures grew methicillin-resistant Staphylococcus aureus (MRSA). Transesophageal echocardiography (TEE) showed a 1.0×1.0 cm mobile vegetation involving the eustachian valve (EV), confirming EV endocarditis. She remained bacteraemic for 18 days despite being on vancomycin with appropriate blood levels. Vancomycin was switched to daptomycin and ceftaroline, which cleared her cultures. Repeat TEE showed improved vegetation size. Our case highlights the rarity and management of EV endocarditis and the importance of synergy for treatment of persistent MRSA bacteraemia.


Assuntos
Endocardite Bacteriana , Endocardite , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Antibacterianos/uso terapêutico , Endocardite/tratamento farmacológico , Endocardite Bacteriana/diagnóstico por imagem , Endocardite Bacteriana/tratamento farmacológico , Feminino , Humanos , Pessoa de Meia-Idade , Diálise Renal , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico
16.
J Vet Diagn Invest ; 33(4): 655-663, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34075814

RESUMO

The use of oral fluid (OF) to detect zoonotic pathogens in pigs has been only scarcely assessed. We evaluated OF as a potential specimen for detection by culture of methicillin-resistant Staphylococcus aureus (MRSA) and Yersinia enterocolitica, and the detection of antibodies against Salmonella spp. and hepatitis E virus (HEV) using commercial ELISAs. Samples from 33 pig farms were collected at the beginning and end of the fattening period. Results of the OF samples were compared with the results of serum samples and nasal swabs from individual pigs and pen floor fecal samples, using the Cohen kappa (κ) and the McNemar test. For Salmonella spp. antibodies, OF samples were negative, although the corresponding serum samples were positive. The detection of HEV antibodies in sera and OF had agreement at the first sampling, and poor and significant agreement at the second sampling (κ = 0.185, McNemar p = 0.238; κ = 0.088, McNemar p < 0.001). At both sampling times, the detection of MRSA in nasal swabs and OF showed agreement (κ = 0.466, McNemar p = 0.077; κ = 0.603, McNemar p = 1); agreement was seen for the detection of Y. enterocolitica in fecal and OF samples (κ = 0.012, McNemar p = 0.868; κ = 0.082, McNemar p = 0.061, respectively). According to the McNemar test, the use of pen-based OFs is more feasible for the detection of MRSA and Y. enterocolitica by culture than is detection of antibodies by commercial ELISA.


Assuntos
Hepatite E/veterinária , Saliva/microbiologia , Salmonelose Animal/epidemiologia , Infecções Estafilocócicas/veterinária , Doenças dos Suínos/epidemiologia , Yersiniose/veterinária , Animais , Hepatite E/diagnóstico , Hepatite E/epidemiologia , Hepatite E/microbiologia , Vírus da Hepatite E/isolamento & purificação , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Prevalência , Salmonella/isolamento & purificação , Salmonelose Animal/diagnóstico , Salmonelose Animal/microbiologia , Estudos Soroepidemiológicos , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Sus scrofa , Suínos , Doenças dos Suínos/diagnóstico , Doenças dos Suínos/microbiologia , Suíça/epidemiologia , Yersiniose/diagnóstico , Yersiniose/epidemiologia , Yersiniose/microbiologia , Yersinia enterocolitica/isolamento & purificação
17.
BMC Musculoskelet Disord ; 22(1): 530, 2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34107951

RESUMO

Septic arthritis (SA) represents a medical emergency that needs immediate diagnosis and urgent treatment. Despite aggressive treatment and rapid diagnosis of the causative agent, the mortality and lifelong disability, associated with septic arthritis remain high as close to 11%. Moreover, with the rise in drug resistance, the rates of failure of conventional antibiotic therapy have also increased. Among the etiological agents frequently isolated from cases of septic arthritis, Staphylococcus aureus emerges as a dominating pathogen, and to worsen, the rise in methicillin-resistant S. aureus (MRSA) isolates in bone and joint infections is worrisome. MRSA associated cases of septic arthritis exhibit higher mortality, longer hospital stay, and higher treatment failure with poorer clinical outcomes as compared to cases caused by the sensitive strain i.e methicillin-sensitive S. aureus (MSSA).In addition to this, equal or even greater damage is imposed by the exacerbated immune response mounted by the patient's body in a futile attempt to eradicate the bacteria. The antibiotic therapy may not be sufficient enough to control the progression of damage to the joint involved thus, adding to higher mortality and disability rates despite the prompt and timely start of treatment. This situation implies that efforts and focus towards studying/understanding new strategies for improved management of sepsis arthritis is prudent and worth exploring.The review article aims to give a complete insight into the new therapeutic approaches studied by workers lately in this field. To the best of our knowledge studies highlighting the novel therapeutic strategies against septic arthritis are limited in the literature, although articles on pathogenic mechanism and choice of antibiotics for therapy, current treatment algorithms followed have been discussed by workers in the past. The present study presents and discusses the new alternative approaches, their mechanism of action, proof of concept, and work done so far towards their clinical success. This will surely help to enlighten the researchers with comprehensive knowledge of the new interventions that can be used as an adjunct therapy along with conventional treatment protocol for improved success rates.


Assuntos
Artrite Infecciosa , Staphylococcus aureus Resistente à Meticilina , Infecções Estafilocócicas , Antibacterianos/uso terapêutico , Artrite Infecciosa/diagnóstico , Artrite Infecciosa/tratamento farmacológico , Artrite Infecciosa/epidemiologia , Humanos , Estudos Retrospectivos , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/epidemiologia , Staphylococcus aureus
19.
Anal Chem ; 93(24): 8544-8552, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34097376

RESUMO

Bacterial infection is one of the major causes of human death worldwide. To prevent bacterial infectious diseases from spreading, it is of critical importance to develop convenient, ultrasensitive, and cost-efficient methods for bacteria detection. Here, an electrochemical detector of a functional two-dimensional (2D) metal-organic framework (MOF) nanozyme was developed for the sensitive detection of pathogenic Staphylococcus aureus. A dual recognition strategy consisting of vancomycin and anti-S. aureus antibody was proposed to specifically anchor S. aureus. The 2D MOFs with excellent peroxidase-like activity can efficiently catalyze o-phenylenediamine to 2,2-diaminoazobenzene, which is an ideal electrochemical signal readout for monitoring the bacteria concentration. Under optimal conditions, the present bioassay provides a wide detection range of 10-7.5 × 107 colony-forming units CFU/mL with a detection limit of 6 CFU/mL, which is better than most of the previous reports. In addition, the established electrochemical sensor can selectively and accurately identify S. aureus in the presence of other bacteria. The present work provides a new pathway for sensitive and selective detection of S. aureus and presents a promising potential in the realm of clinical diagnosis.


Assuntos
Técnicas Biossensoriais , Estruturas Metalorgânicas , Infecções Estafilocócicas , Catálise , Técnicas Eletroquímicas , Humanos , Limite de Detecção , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus , Vancomicina
20.
BMJ Case Rep ; 14(5)2021 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-33975834

RESUMO

A woman in her 60s with diabetes presented to our institution with altered mental status. Preceding symptoms included headaches, nausea and vomiting. One month prior to presentation, she cut her left thumb and developed a pustule, which she occasionally manipulated with a non-sterile needle. On arrival, the patient was in shock, with a Glasgow Coma Scale of 3, requiring emergent intubation and intensive care unit admission. Her initial imaging studies revealed a large pericardial effusion and cerebral subcortical hypodensities. She suffered from a cardiopulmonary arrest with return of spontaneous circulation, with bedside echocardiogram revealing cardiac tamponade. She underwent emergent pericardiocentesis which revealed purulent drainage. Blood, pericardial fluid, cerebrospinal fluid, sputum and urine cultures returned positive for methicillin-sensitive Staphylococcus aureus The hospital course was further complicated by refractory septic shock and fulminant multiorgan failure, ultimately leading to her demise.


Assuntos
Tamponamento Cardíaco , Infecções Estafilocócicas , Feminino , Humanos , Meticilina , Pericardiocentese , Infecções Estafilocócicas/complicações , Infecções Estafilocócicas/diagnóstico , Staphylococcus aureus
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