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1.
Vet Microbiol ; 239: 108495, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31767098

RESUMO

Bovine gammaherpesvirus type 4 (BoHV-4) is increasingly related with reproductive disease in cattle, but its epidemiology is not fully understood. We monitored the serological response and shedding of BoHV-4 in a positive dairy cattle farm with metritis. First, we performed an ELISA to detect BoHV-4 antibodies in all the animals (n = 104). Afterwards, ten seronegative heifers introduced in the production lot and sera samples were monthly taken for four months and then 6-10 months after introduction to detect BoHV-4 antibodies by ELISA. Moreover, a vaginal swab was taken after calving to detect BoHV-4 by PCR. Concurrently, a weekly collection of vaginal and nasal swabs and milk was performed during the first month post-partum in multiparous cows with metritis (n = 14), heifers with metritis (n = 4), heifers without metritis but positive to BoHV-4 (ELISA or PCR) (n = 2) and multiparous cows without metritis (n = 3). Seropositivity was higher in older animals and in the production lot. Three heifers which shed BoHV-4 after parturition resulted seronegative at first but eventually seroconverted. In the same vein, most heifers seroconverted after 6-10 months in the production lot (8/10). Multiparous cows shed virus by various routes: 13/14 (93 %) in vaginal secretions, 7/14 (50 %) in nasal exudates and 7/14 (50 %) in milk. However, in the other groups, shedding was only detected in vaginal swabs from the first week post-partum. Our study describes BoHV-4 shedding in field conditions. Seronegative animals may become horizontally infected when moved to a contaminated environment.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 4/fisiologia , Infecções Tumorais por Vírus/veterinária , Eliminação de Partículas Virais , Animais , Bovinos , Feminino , Infecções por Herpesviridae/sangue , Infecções por Herpesviridae/imunologia , Infecções por Herpesviridae/virologia , Período Pós-Parto , Soroconversão , Infecções Tumorais por Vírus/sangue , Infecções Tumorais por Vírus/imunologia , Infecções Tumorais por Vírus/virologia , Vagina/virologia
2.
Vet Microbiol ; 237: 108418, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31585637

RESUMO

The prevalence of Marek's disease (MD) caused by Gallid herpesvirus-2 (GaHV-2) has been increasing in chickens in China despite universal vaccination. Among the possible reasons for this trend, of Reticuloendotheliosis virus (REV) contamination in vaccines could lead to co-infection and reduce the vaccine efficacy. Here, we report the epidemiological findings of our continuous surveillance of MD, and an examination of the effects of REV and/or GaHV-2 co-infection. A total of 1230 samples were collected between 2011 and 2015 from 305 flocks covering many of the chicken-raising regions of China. Among these, 606 samples were determined to be GaHV-2-positive, 13.0% of which were found to be co-infected with REV from 18.8% of the flocks. One GaHV-2 strain (HS/1412), a REV strain (HS/1412R), and a GaHV-2 and REV-co-infected strain (HS/1412 GR) were isolated from different chickens of a GaHV-2 and REV co-infected flock. Pathogenicity tests showed that HS/1412 and HS/1412 GR caused disease in all chickens and that HS/1412R induced morbidity in 84.6% of the infected chickens. HS/1412 GR induced 100% mortality and 76.9% tumor formation, which were significantly higher frequencies than those observed with strain HS/1412 (38.5% and 15.4%, respectively) and HS/1412R (0% and 0%). These results indicate that co-infection with GaHV-2 and REV might explain the persistent, sporadic outbreaks of neoplastic disease in some commercial flocks, resulting in a significant economic burden to the poultry industry of China.


Assuntos
Galinhas , Coinfecção/veterinária , Doença de Marek/complicações , Neoplasias/veterinária , Doenças das Aves Domésticas/virologia , Infecções por Retroviridae/veterinária , Animais , China/epidemiologia , Coinfecção/epidemiologia , Coinfecção/virologia , Herpesvirus Galináceo 2/isolamento & purificação , Doença de Marek/epidemiologia , Neoplasias/epidemiologia , Neoplasias/virologia , Vírus da Reticuloendoteliose/isolamento & purificação , Infecções por Retroviridae/complicações , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/virologia , Infecções Tumorais por Vírus/complicações , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/veterinária , Infecções Tumorais por Vírus/virologia
3.
J Vet Diagn Invest ; 31(5): 761-765, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31378167

RESUMO

We investigated the histologic findings and viral antigen distribution in 3 cases of natural coinfection of layer hens with subgroup J avian leukosis virus (ALV-J), Marek's disease virus (MDV), and reticuloendotheliosis virus (REV) in hens. At autopsy, diseased hens were found to have hepatosplenomegaly and thickened proventriculi, with white tumor nodules in the liver, spleen, lung, kidney, and ovary. Microscopically, most tissues had been infiltrated by neoplastic lymphocytes; the spleen, lung, proventriculus, heart, and liver had been infiltrated by both neoplastic lymphocytes and myeloblastic cells and/or primitive reticular cells. Fluorescence multiplex immunohistochemistry staining revealed ALV-J, MDV, and REV antigens co-expressed in the same tissue, even the same cell.


Assuntos
Leucose Aviária/virologia , Galinhas , Coinfecção/veterinária , Doença de Marek/virologia , Doenças das Aves Domésticas/virologia , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Antígenos Virais/análise , Leucose Aviária/imunologia , Leucose Aviária/patologia , Vírus da Leucose Aviária/fisiologia , Coinfecção/imunologia , Coinfecção/patologia , Coinfecção/virologia , Feminino , Herpesvirus Galináceo 2/fisiologia , Doença de Marek/imunologia , Doença de Marek/patologia , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/patologia , Vírus da Reticuloendoteliose/fisiologia , Infecções por Retroviridae/imunologia , Infecções por Retroviridae/patologia , Infecções por Retroviridae/virologia , Infecções Tumorais por Vírus/imunologia , Infecções Tumorais por Vírus/patologia , Infecções Tumorais por Vírus/virologia
4.
J Avian Med Surg ; 33(2): 141-149, 2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-31251501

RESUMO

Avian polyomavirus disease and psittacine beak and feather disease (PBFD) are both contagious viral diseases in psittacine birds with similar clinical manifestations and characterized by abnormal feathers. To determine the prevalence of Aves polyomavirus 1 (APyV) and beak and feather disease virus (BFDV) in captive, exotic psittacine birds in Chile, feathers from 250 psittacine birds, representing 17 genera, were collected and stored during the period 2013-2016. Polymerase chain reaction testing was used to detect APyV and BFDV were detected in feather bulb samples. The results indicated that 1.6% (4/250) of the samples were positive for APyV, 23.2% (58/250) were positive to BFDV, and 0.8% (2/250) were positive to both APyV and BFDV. This is the first report, to our knowledge, of APyV and BFDV prevalence in captive, exotic psittacine birds in South America. Analysis of 2 Chilean partial sequences of the gene encoding agnoprotein 1a (APyV) and the replication-associated protein (BFDV) extends the knowledge of genomic variability for both APyV and BFDV isolates and their spectrum of hosts. No geographical marker was detected for the local isolates.


Assuntos
Doenças das Aves/virologia , Infecções por Circoviridae/veterinária , Circovirus/isolamento & purificação , Animais de Estimação/virologia , Polyomavirus/isolamento & purificação , Psittaciformes , Animais , Doenças das Aves/epidemiologia , Chile/epidemiologia , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/virologia , Circovirus/genética , Filogenia , Polyomavirus/classificação , Infecções por Polyomavirus/epidemiologia , Infecções por Polyomavirus/veterinária , Infecções por Polyomavirus/virologia , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/veterinária , Infecções Tumorais por Vírus/virologia
5.
Res Vet Sci ; 125: 89-93, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31176263

RESUMO

Feline Leukemia Virus (FeLV) and Feline Immunodeficiency Virus (FIV) are two prevalent transmittable diseases for domestic cats. This paper reports the frequency of these two diseases compared globally across Gross Domestic Product (GDP) at purchasing power parity per capita (PPP). Information around FeLV and FIV rates of infection in specific locations around the world was analyzed from 47 published articles. Results show that based on the data available, the statistical model indicates that the highest percentage of FeLV or FIV infected cats live in areas of lower PPP (p ≤.001) with a decreasing rate of infection of FeLV and FIV with increasing income. Two theories for this could be that the lower PPP locations in this study were also in areas of greater feral cat and cat colony populations, as well as were areas with less emphasis on animal welfare and animal control programs. Additional research should be conducted to strengthen the study size in South America and Africa before further conclusions can be drawn.


Assuntos
Doenças do Gato/epidemiologia , Guanosina Difosfato , Vírus da Imunodeficiência Felina/fisiologia , Infecções por Lentivirus/veterinária , Vírus da Leucemia Felina/fisiologia , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Doenças do Gato/virologia , Gatos , Incidência , Infecções por Lentivirus/epidemiologia , Infecções por Lentivirus/virologia , Prevalência , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/virologia , Estudos Soroepidemiológicos , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologia
6.
J Comp Pathol ; 169: 1-4, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31159945

RESUMO

An 11-month-old female Japanese macaque (Macaca fuscata), born in captivity in a research institute, suddenly died without clinical signs. Necropsy examination revealed a nodular mass protruding from the left ventral aspect of the larynx, compressing the epiglottis anteriorly. Histopathologically, the laryngeal mass was comprised of medium- to large-sized atypical cells. Immunohistochemically, these were positive for CD20 and partially positive for CD79α. Among the atypical cells were CD3+ T cells and CD68+ histiocytes. Based on the findings, this case was diagnosed as T-cell/histiocyte-rich large B-cell lymphoma. Epstein-Barr virus (EBV)-encoded small RNAs were frequently detected in the atypical cells by in-situ hybridization, which was consistent with the finding that the macaque was seropositive for EBV antigen. This is the first report showing the potential association of simian lymphocryptovirus, the simian homologue of EBV, with lymphoma in a juvenile non-human primate.


Assuntos
Infecções por Herpesviridae/veterinária , Linfoma Difuso de Grandes Células B/veterinária , Doenças dos Primatas/patologia , Doenças dos Primatas/virologia , Infecções Tumorais por Vírus/veterinária , Animais , Feminino , Histiócitos/patologia , Lymphocryptovirus , Linfócitos T/patologia
7.
Artigo em Inglês | MEDLINE | ID: mdl-30961813

RESUMO

A cross-sectional study was conducted in 274 cats for determination of FeLV antigenemia and FIV seropositivity and factors associated with those infections in cats presented at the Veterinary Hospital of the Santa Catarina State University - UDESC (Brazil). Apparent prevalence for sick cats at the hospital population was 28.41% (95%CI 21.88-34.94%) for FeLV, 7.65% (95%CI 3.71-11.50%) for FIV and 2.18% (95%CI 0.56-5.47%) for both viruses. For healthy cats, the apparent prevalence was 9.89% (95%CI 3.75-16.02%) for FeLV, 2.20% (95%CI 0.34-7.75%) for FIV by immunoassay (ELISA). Average age for FeLV- and FIV-positive individuals was 38.32 and 64.25 months, respectively. Behavior such as aggressiveness and sex (male) were both associated with increased odds of result positivity test for FeLV and FIV; older animals were also associated with FIV test results. A very small proportion of the animals were vaccinated against FeLV and none against FIV. Most of the animals were adopted from shelters or rescued from streets, living with multiple cats that had access to outdoors. The high prevalence of FeLV suggests a need for better control strategies against this disease.


Assuntos
Doenças do Gato/epidemiologia , Síndrome de Imunodeficiência Adquirida Felina/epidemiologia , Vírus da Imunodeficiência Felina/imunologia , Vírus da Leucemia Felina/imunologia , Leucemia Felina/epidemiologia , Infecções Tumorais por Vírus/veterinária , Animais , Antígenos Virais/sangue , Antígenos Virais/imunologia , Comportamento Animal/fisiologia , Brasil/epidemiologia , Doenças do Gato/virologia , Gatos , Estudos Transversais , Ensaio de Imunoadsorção Enzimática/veterinária , Síndrome de Imunodeficiência Adquirida Felina/virologia , Feminino , Leucemia Felina/virologia , Masculino , Prevalência , Fatores de Risco
8.
Comp Immunol Microbiol Infect Dis ; 63: 97-103, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30961825

RESUMO

Bovine gammaherpesvirus 4 (BoHV4) is a member of the family Herpesviridae. In Argentina, BoHV4 was isolated and characterized in 2007 from samples of aborted cows. Argentinean isolates are highly divergent and are classified as: Genotype 1(Movar-like), Genotype 2 (DN599-like) and Genotype 3 (a novel group). The aim of this study was to comparatively evaluate the biological characteristics of six Argentinean BoHV4 field isolates in cell lines from different origins. All strains induced productive infection in the cell lines used, with different degrees of permissiveness. A direct relationship among the times of appearance of cytopathic effect, the growth kinetics, the size of the lysis plaques and the virulent-like behaviour in vitro could not be established. However, although slight, there are differences in the biological behaviour of the BoHV4 fields isolates analyzed. This variability is independent of their genetic classification but would be conditioned by the nature of the infected cells.


Assuntos
Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 4/fisiologia , Infecções Tumorais por Vírus/veterinária , Replicação Viral/fisiologia , Animais , Argentina , Bovinos , Linhagem Celular , Efeito Citopatogênico Viral , Cães , Células HeLa , Células Hep G2 , Herpesvirus Bovino 4/genética , Herpesvirus Bovino 4/isolamento & purificação , Humanos , Células Madin Darby de Rim Canino , Células Vero
9.
Trop Anim Health Prod ; 51(7): 2095-2098, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30997630

RESUMO

Enzootic nasal tumor (ENT) is a contagious neoplasm associated with enzootic nasal tumor virus (ENTV), which may induce disease in sheep (ENTV-1) and goats (ENTV-2). This study aimed to describe the occurrence of ENT in two Texel sheep (Ovis aries) from a 75-sheep flock, located in the city of Gravataí, southern Brazil. Animals used to be purchased from different origins, and no specific tests for disease monitoring or quarantine procedure were performed. Affected animals presented respiratory distress, anorexia with severe weight loss, and mucopurulent unilateral nasal discharge. Necropsy was performed in both animals and nasal cavity masses were observed. Histopathological analysis demonstrated an epithelial neoplasm compatible with nasal adenocarcinoma. PCR using a protocol that amplifies a 591 bp sequence of 5'LTR-gag region of ENTV1 was performed followed by DNA sequencing. Both samples were positive, and the sequences obtained presented highest identity (97%) with ENTV strain TN28 (GenBank accession number MH899613) detected in a Texel sheep from Scotland. This is the first report of ENTV-1 leading to enzootic nasal tumor in sheep in Latin America, which confirms the presence of the retrovirus in sheep flocks in the Brazilian territory.


Assuntos
Neoplasias Nasais/diagnóstico , Neoplasias Nasais/veterinária , Infecções Tumorais por Vírus/diagnóstico , Infecções Tumorais por Vírus/veterinária , Animais , Betaretrovirus , Brasil , Doenças das Cabras/virologia , Cabras/virologia , Neoplasias Nasais/virologia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Ovinos/virologia , Doenças dos Ovinos/virologia , Infecções Tumorais por Vírus/virologia
10.
Arq. bras. med. vet. zootec. (Online) ; 71(2): 447-454, mar.-abr. 2019. tab, ilus
Artigo em Português | LILACS, VETINDEX | ID: biblio-1011258

RESUMO

FIV e FeLV são retrovírus associados principalmente com neoplasias. Dois testes rápidos são disponibilizados no Brasil para o diagnóstico dessas infecções: um kit de imunocromatografia de fluxo bidirecional (SNAP® Combo IDEXX) e um kit de imunocromatografia de fluxo lateral unidirecional (ALERE/BIONOTE Anigen Rapid). O objetivo deste estudo foi comparar o teste SNAP® com o teste ALERE. Amostras de sangue de 178 gatos foram testadas utilizando-se ambos os kits. A reação em cadeia de polimerase em tempo real (qPCR) foi empregada como método confirmatório para todos os resultados. O teste SNAP® apresentou sensibilidade e especificidade de 100% para FIV; a sensibilidade e a especificidade do teste ALERE foram de 96,15% e 98,68%, respectivamente. A sensibilidade e a especificidade para o FeLV foram de 93,02% e 96,30% para o teste SNAP® e de 90,70% e 97,78% para o teste ALERE. Ainda em relação ao FeLV, três amostras com resultado positivo na qPCR obtiveram resultado falso-negativo em ambos os testes. Não houve diferença estatisticamente significante entre os métodos. Considerando a qPCR como padrão-ouro, o teste SNAP® apresentou maior sensibilidade e especificidade para o FIV, e o teste ALERE apresentou maior especificidade para o FeLV. Os resultados mostraram uma boa correlação entre os testes.(AU)


FIV and FeLV are Retrovirus associated mainly with feline neoplasms. Two point-of-care tests are commercially available in Brazil for diagnosis of these infections: a bidirectional flow immunochromatography kit (IDEXX SNAP ® Combo) and a lateral unidirectional flow immunochromatography kit (ALERE/BIONOTE Anigen Rapid). The aim of this study was to compare SNAP ® and ALERE tests. Blood samples obtained from 178 cats were evaluated using both tests. Quantitative real-time polymerase chain reaction (qPCR) was used as confirmatory test for all samples. The sensitivity and specificity of SNAP ® test was 100% for FIV, and for ALERE test was 96.15% and 98.68%, respectively. The sensitivity and specificity for FeLV was 93.02% and 96.30% for SNAP ® test and 90.70% and 97.78% for ALERE test. Three samples with a qPCR positive result for FeLV obtained a false negative result in both SNAP ® and ALERE tests. There was no statistically significant difference between the two methods. Considering qPCR as gold standard method, the SNAP® test showed higher sensitivity and specificity for FIV, and the ALERE test presented higher specificity for FeLV. The results showed good agreement among the tests.(AU)


Assuntos
Animais , Gatos , Infecções Tumorais por Vírus/diagnóstico , Infecções Tumorais por Vírus/veterinária , Testes Sorológicos/veterinária , Doenças do Gato/diagnóstico , Infecções por Lentivirus/diagnóstico , Leucemia Felina/diagnóstico , Infecções por Retroviridae/diagnóstico , Infecções por Retroviridae/veterinária , Reação em Cadeia da Polimerase/veterinária , Cromatografia de Afinidade/veterinária , Gammaretrovirus , Vírus da Imunodeficiência Felina
11.
Vet Res ; 50(1): 13, 2019 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-30777128

RESUMO

Equine herpesvirus type 5 (EHV5) is a ubiquitous, yet obscure pathogen in the horse population and is commonly associated with fatal equine multinodular pulmonary fibrosis (EMPF). To date, little is known about the precise pathogenesis of EHV5. Here, we evaluated the dynamics of EHV5 infection in representative ex vivo and in vitro equine models, using immunofluorescence staining and virus titration. EHV5 was unable to infect epithelial cells lining the mucosa of nasal and tracheal explants. Similarly, primary equine respiratory epithelial cells (EREC) were not susceptible to EHV5 following inoculation at the apical or basolateral surfaces. Upon direct delivery of EHV5 particles to lung explants, few EHV5-positive cell clusters were observed at 72 hours post-inoculation (hpi). These EHV5-positive cells were identified as cytokeratin-positive alveolar cells. Next, we examined the potential of EHV5 to infect three distinct equine PBMC populations (CD172a+ monocytes, CD3+ T lymphocytes and Ig light chain+ B lymphocytes). Monocytes did not support EHV5 replication. In contrast, up to 10% of inoculated equine T and B lymphocytes synthetized intracellular viral antigens 24 hpi and 72 hpi, respectively. Still, the production of mature virus particles was hampered, as we did not observe an increase in extracellular virus titer. After reaching a peak, the percentage of infected T and B lymphocytes decayed, which was partly due to the onset of apoptosis, but not necrosis. Based on these findings, we propose a model for EHV5 pathogenesis in the horse. Uncovering EHV5 pathogenesis is the corner step to finally contain or even eradicate the virus.


Assuntos
Gammaherpesvirinae/fisiologia , Infecções por Herpesviridae/veterinária , Doenças dos Cavalos/virologia , Infecções Tumorais por Vírus/veterinária , Animais , Linhagem Celular , Células Epiteliais , Imunofluorescência/veterinária , Infecções por Herpesviridae/virologia , Cavalos , Técnicas In Vitro , Infecções Tumorais por Vírus/virologia , Carga Viral/veterinária
12.
Vet Res ; 50(1): 11, 2019 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-30736853

RESUMO

Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that is widespread in cattle. However, only a few studies about the pathogenesis of BoHV-4 primary infection have been reported. In the present study, ex vivo models with bovine nasal and tracheal mucosa explants were used to study the cellular BoHV-4-host interactions. Infection was observed in nasal but not in tracheal epithelial cells. To find a possible correlation between the integrity and restricted infection of the respiratory epithelium, both nasal mucosal and tracheal explants were treated with EGTA, a drug that disrupts the intercellular junctions, before inoculation. The infection was analyzed based on the number of plaques, plaque latitude and number of infected single cells, as determined by immunofluorescence. BoHV-4 infection in nasal mucosal explants was enhanced upon opening the tight junctions with EGTA. Infection in tracheal explants was only found after treatment with EGTA. In addition, primary bovine respiratory epithelial cells (BREC) were isolated, grown at the air-liquid interface and infected either at the apical or basolateral side by BoHV-4. The results showed that BoHV-4 preferentially bound to and entered BREC at the basolateral surfaces of both nasal and tracheal epithelial cells. The percentage of BoHV-4 infection was significantly increased both from nasal and tracheal epithelial cells after treatment with EGTA, which indicates that the BoHV-4 receptor is mainly located at the basolateral surface of these cells. Thus, our findings demonstrate that integrity of the respiratory epithelium is crucial in the host's innate defense against primary BoHV-4 infections.


Assuntos
Doenças dos Bovinos/fisiopatologia , Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 4/fisiologia , Infecções Tumorais por Vírus/veterinária , Animais , Bovinos , Doenças dos Bovinos/virologia , Infecções por Herpesviridae/fisiopatologia , Infecções por Herpesviridae/virologia , Mucosa Respiratória/fisiopatologia , Mucosa Respiratória/virologia , Infecções Tumorais por Vírus/fisiopatologia , Infecções Tumorais por Vírus/virologia
13.
J Comp Pathol ; 166: 20-28, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30691602

RESUMO

Lymphoma is the most important haemopoietic tumour in cats and has been associated with feline leukaemia virus (FeLV) infection. In Brazil, no studies have established a correlation between FeLV infection and lymphoma. The aim of this study was to characterize lymphomas arising in cats in Brazil anatomically and microscopically, and to correlate these data with FeLV infection as determined by immunohistochemistry for the FeLV gp70 antigen. Fifty-three cats with lymphoma were evaluated. The mean age of junior, prime, mature, senior and geriatric cats was 1.65 years, 4.34 years, 8 years, 12.14 years and 15.5 years, respectively. The anatomical types of lymphoma were multicentric (43.4%, 23/53), mediastinal (33.96%, 18/53), renal (11.32%, 6/53), hepatic (5.66%, 3/53), nodal (3.77%, 2/53) and alimentary (1.89 %, 1/53). The histological types were small noncleaved-cell (33.96%, 18/53), mixed diffuse (22.64%, 12/53), immunoblastic (15.11%, 8/53), lymphoblastic (11, 32%, 6/53), small lymphocytic (9.43%, 5/53), small cleaved-cell (3.77%, 2/53) and large cell lymphomas (3.77%, 2/3). Immunopositivity for FeLV was observed in 56.6% (30/53) of the samples. FeLV positivity was equally distributed between the genders, but predominated in junior and prime cats. The degree of association between lymphoma and FeLV infection in Brazil was higher than that found in other countries, demonstrating the need to prevent and control the factors associated with infection.


Assuntos
Doenças do Gato/virologia , Linfoma/veterinária , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Brasil , Gatos , Feminino , Vírus da Leucemia Felina , Masculino
14.
Poult Sci ; 98(6): 2432-2438, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30668827

RESUMO

Reticuloendotheliosis virus (REV) causes an immunosuppressive, runting, and oncogenic disease in poultry, posing a significant threat to the poultry industry. In Thailand, an unidentified disease associated with runting-stunting syndrome and neoplasia, resembling REV infection, has been continuously observed in several chicken farms. However, REV infection in Thailand has never been reported. In this study, we investigated the occurrence and genetic characteristics of REVs in chickens in Thailand from 2013 to 2016. Of the 130 clinical samples obtained from 29 chicken farms from 9 provinces located in the major chicken-raising regions of Thailand, including the central, eastern, northern, and northeastern parts of Thailand, 51 samples (39.23%) and 21 farms (72.41%) were REV-positive. REV-positive samples were detected in all 9 provinces tested. Our results demonstrated that REV was extensively distributed in the major chicken-raising regions of Thailand. Phylogenetic analysis of the whole genome sequence showed that Thai REV was most closely related to Chinese, Taiwanese, and the US REV strains isolated from different avian species and clustered into REV subtype III. This finding indicates that REV subtype III was predominantly circulated in Thai chicken flocks. This study is the first report on REV infection in chickens in Thailand. Our findings raise the awareness of REV as another causative agent of runting and oncogenic disease in chickens in Thailand and highlight the wide distribution of REV infection among chickens worldwide.


Assuntos
Galinhas , Doenças das Aves Domésticas/epidemiologia , Vírus da Reticuloendoteliose/fisiologia , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Variação Genética , Genoma Bacteriano , Filogenia , Doenças das Aves Domésticas/virologia , Prevalência , Vírus da Reticuloendoteliose/classificação , Vírus da Reticuloendoteliose/genética , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/virologia , Estudos Soroepidemiológicos , Tailândia/epidemiologia , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologia
15.
Arch Virol ; 164(3): 767-774, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30663022

RESUMO

Fecal samples collected from free-ranging Atlantic bottlenose dolphins (BDs) in the Indian River Lagoon of Florida were processed for viral discovery using a next-generation sequencing (NGS) approach. A 693-bp contig identified in the NGS data was nearly identical to the partial L1 gene sequence of a papillomavirus (PV) previously found in a penile papilloma in a killer whale (Orcinus orca). Based on this partial bottlenose dolphin papillomavirus (BDPV) sequence, a nested inverse PCR and primer-walking strategy was employed to generate the complete genome sequence. The full BDPV genome consisted of 7299 bp and displayed a typical PV genome organization. The BDPV E6 protein contained a PDZ-binding motif, which has been shown to be involved in carcinogenic transformation involving high-risk genital human PVs. Screening of 12 individual fecal samples using a specific endpoint PCR assay revealed that the feces from a single female BD displaying a genital papilloma was positive for the BDPV. Genetic analysis indicated that this BDPV (Tursiops truncatus papillomavirus 8; TtPV8) is a new type of Dyopipapillomavirus 1, previously sequenced from an isolate obtained from a penile papilloma in a harbor porpoise (Phocoena phocoena). Although only a partial L1 sequence has been determined for a PV detected in a killer whale genital papilloma, our finding of a nearly identical sequence in an Atlantic BD may indicate that members of this viral species are capable of host jumping. Future work is needed to determine if this virus is a high-risk PV that is capable of inducing carcinogenic transformation and whether it poses a significant health risk to wild delphinid populations.


Assuntos
Golfinho Nariz-de-Garrafa/virologia , Papillomaviridae/genética , Infecções Tumorais por Vírus/veterinária , Animais , Feminino , Florida , Genômica , Papillomaviridae/classificação , Papillomaviridae/isolamento & purificação , Filogenia , Rios/virologia , Infecções Tumorais por Vírus/virologia , Proteínas Virais/genética
16.
Vet Microbiol ; 229: 130-137, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30642588

RESUMO

In the past, bovine herpesvirus 4 (BoHV-4) has been suggested to be associated with metritis and endometritis. However, not many field studies investigated the association between BoHV-4 and subclinical endometritis (SCE). In the present study, the association between the intrauterine presence of BoHV-4 and SCE diagnosed during artificial insemination (AI) was examined on two dairy farms in Belgium. An immunoperoxidase monolayer assay (IPMA) and an enzyme-linked immuno sorbent assay (ELISA) were used to screen the serum for anti-BoHV-4 antibodies. A SYBR green based one step real time qPCR was used to detect and quantify BoHV-4 (ORF20) in nasal, uterine and vaginal samples collected at AI. A reverse transcription qPCR (RT-qPCR) was used to detect mRNA (gB) as proof of a productive BoHV-4 infection. BoHV-4 was detected in 39.4% (farm A)/23.8% (farm B) of the nasal samples, 48.5% (farm A)/19.0% (farm B) of the uterine samples and 51.5% (farm A)/42.9% (farm B) of the vaginal samples. Active replication was only detected in farm A in 38.5% of the BoHV-4 positive nasal samples and in 5.9% positive cases of the vaginal samples. The prevalence of SCE diagnosed at AI was 45.5% and 42.9% in farm A and farm B, respectively. The presence of SCE was associated with a reduced pregnancy outcome at artificial insemination (AI) (P<0.001). The occurrence of SCE at AI was not associated with the presence of latent or productive BoHV4 infections in the uterus nor in the vagina and nose (P>0.05).


Assuntos
Doenças dos Bovinos/virologia , Endometrite/veterinária , Infecções por Herpesviridae/veterinária , Herpesvirus Bovino 4/isolamento & purificação , Inseminação Artificial/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Bélgica/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/transmissão , DNA Viral/isolamento & purificação , Endometrite/epidemiologia , Endometrite/virologia , Feminino , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/virologia , Inseminação Artificial/efeitos adversos , Estudos Soroepidemiológicos , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologia
17.
Arch Virol ; 164(3): 707-716, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30604242

RESUMO

Enzootic nasal adenocarcinoma (ENA) is a contagious neoplasm of sheep and goats, associated with the oncogenic retroviruses enzootic nasal tumor virus (ENTV) 1 and 2, respectively. It appears to be common in countries with substantial small ruminant-production. ENA diagnosis in goats is based on autopsy and histopathology, and there is no real-time PCR method available for ENTV-2 detection. Here, a novel one-tube real-time RT-PCR (RT-qPCR) method for the detection and quantification of ENTV-2 in nasal swabs is presented. The method targets the env gene/U3 region. For the design of ENTV-2-specific oligonucleotides, molecular characterization of seven Greek ENTV-2 strains was performed. Phylogenetic analysis revealed three distinct phylogenetic clades of ENTV-2 that correlate with the country of sample collection. Evaluation of the analytical performance of the RT-qPCR revealed an amplification efficiency of 92.8% and a linear range of quantification between 2 × 108 and 2 × 102 RNA transcripts. Analysis of nasal swabs from 23 histopathologically confirmed, naturally occurring ENA cases via RT-qPCR yielded positive results. Moreover, modification of the method for use in a real-time PCR (qPCR) assay enables detection of proviral DNA in tumor specimens. Both methods are highly specific and can be used for the confirmation of ENA-suspected cases. Future applications could include ante-mortem diagnosis, verification of the ENTV-2-free status in animal trade, disease surveillance, and control programs.


Assuntos
Betaretrovirus/isolamento & purificação , Doenças das Cabras/virologia , Neoplasias Nasais/veterinária , Reação em Cadeia da Polimerase em Tempo Real/métodos , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Betaretrovirus/classificação , Betaretrovirus/genética , Doenças das Cabras/diagnóstico , Cabras , Neoplasias Nasais/diagnóstico , Neoplasias Nasais/virologia , Filogenia , Infecções por Retroviridae/diagnóstico , Infecções por Retroviridae/virologia , Infecções Tumorais por Vírus/diagnóstico , Infecções Tumorais por Vírus/virologia
18.
J Wildl Dis ; 55(1): 113-122, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30124393

RESUMO

The successful reintroduction of Wild Turkeys ( Meleagris gallopavo) to Ontario, Canada, has led to established populations in southern portions of the province and currently allows for biannual hunting seasons. These populations geographically overlap Domestic Turkey farms, an important sector of the provincial agri-food industry. Potential pathogen transmission between Wild Turkeys and Domestic Turkeys ( Meleagris gallopavo) is a concern, because they are susceptible to infection with many of the same pathogens and have direct and indirect contact in outdoor or open farm settings and contaminated environmental substrates. However, data concerning potential poultry pathogens in Wild Turkeys in Canada are scarce. Thus, we assessed the prevalence and geographic distribution of geographically relevant viruses in Ontario Wild Turkeys. Oropharyngeal and cloacal swabs were tested for avian influenza viruses (AIV) by real-time reverse transcriptase (RT)-PCR ( n=207), pooled tissues for lymphoproliferative disease virus (LPDV; n=183) and reticuloendotheliosis virus ( n=119) by PCR, and gross skin lesions by real-time RT-PCR for avian poxvirus ( n=8). We sequenced a fragment of the gag polyprotein (p31) gene of LPDV on a subset ( n=10) of LPDV-positive samples for phylogenetic analysis and tested additional upland game bird species ( n=39) and domestic fowl for LPDV ( n=17). To the best of our knowledge, we document the first detection of LPDV in Wild Turkeys in Canada, with a prevalence of 65% (119/183). Phylogenetic analysis revealed that LPDV sequences from Ontario were genetically similar to other North American strains and did not group into separate clades. Reticuloendotheliosis virus was detected in 4% (5/119) of LPDV-positive Wild Turkeys. Grossly evident skin lesions from five Wild Turkeys tested positive for poxvirus, and all turkeys tested negative for AIV. This study provides evidence of LPDV circulation in Canada and provides a baseline for comparison with future Wild Turkey pathogen surveillance and monitoring in Ontario and elsewhere.


Assuntos
Alpharetrovirus/isolamento & purificação , Doenças das Aves/virologia , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Perus/virologia , Envelhecimento , Animais , Animais Selvagens , Doenças das Aves/epidemiologia , Ontário/epidemiologia , Vigilância da População , Vírus da Reticuloendoteliose Aviária , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/virologia , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologia
19.
Mol Cell Probes ; 43: 80-83, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30414896

RESUMO

Budgerigar fledgling disease virus (BFDV) infection causes sudden death, abdominal distention, and feather abnormality in psittacine birds. In this study, we developed a TaqMan Real-time PCR assay to detect BFDV by targeting a conserved region in VP1 gene. The detection limit of the assay was 30 DNA gene copies, 1000 times more sensitive than conventional PCR. The coefficients of variation were less than 1.09% in either intra- or inter-assays, indicating high reproducibility. By using this method, the prevalence of BFDV in China was evaluated. 56 feces samples were collected from four psittacine birds breeding facilities in China. The results showed 28 out of 56 samples were positive for BFDV in Real-Time PCR assay, while only 19 samples were positive in PCR assay. Three facilities were positive for BFDV with positive rates from 60% to 87.5%. Further sequence analysis of VP1 genes from the positive samples indicated that VP1 genes fell into two different lineages in phylogenetic tree, suggesting that different genotypes BFDV are co-circulating in China.


Assuntos
Melopsittacus/virologia , Infecções por Polyomavirus/epidemiologia , Infecções por Polyomavirus/veterinária , Polyomavirus/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/veterinária , Animais , Fezes/virologia , Infecções por Polyomavirus/virologia , Vigilância da População , Padrões de Referência , Reprodutibilidade dos Testes , Infecções Tumorais por Vírus/virologia
20.
Emerg Infect Dis ; 25(1): 92-101, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30561312

RESUMO

The endangered Florida panther (Puma concolor coryi) had an outbreak of infection with feline leukemia virus (FeLV) in the early 2000s that resulted in the deaths of 3 animals. A vaccination campaign was instituted during 2003-2007 and no additional cases were recorded until 2010. During 2010-2016, six additional FeLV cases were documented. We characterized FeLV genomes isolated from Florida panthers from both outbreaks and compared them with full-length genomes of FeLVs isolated from contemporary Florida domestic cats. Phylogenetic analyses identified at least 2 circulating FeLV strains in panthers, which represent separate introductions from domestic cats. The original FeLV virus outbreak strain is either still circulating or another domestic cat transmission event has occurred with a closely related variant. We also report a case of a cross-species transmission event of an oncogenic FeLV recombinant (FeLV-B). Evidence of multiple FeLV strains and detection of FeLV-B indicate Florida panthers are at high risk for FeLV infection.


Assuntos
Surtos de Doenças/veterinária , Genoma Viral/genética , Vírus da Leucemia Felina/genética , Puma/virologia , Infecções por Retroviridae/veterinária , Infecções Tumorais por Vírus/veterinária , Animais , Gatos , Espécies em Perigo de Extinção , Florida/epidemiologia , Vírus da Leucemia Felina/isolamento & purificação , Filogenia , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/transmissão , Infecções por Retroviridae/virologia , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/transmissão , Infecções Tumorais por Vírus/virologia
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