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1.
Vet Pathol ; 56(5): 789-793, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31221032

RESUMO

Chlamydia pneumoniae is a ubiquitous pathogen causing disease in humans, mammals, birds, reptiles, and amphibians. Since 2012, C. pneumoniae infection has caused neurologic disease and mortality in a breeding colony of endangered Houston toads (Anaxyrus houstonensis) at the Houston Zoo. The purpose of this report is to present the histopathologic and ultrastructural characteristics of C. pneumoniae infection in Houston toads. Fourteen cases were evaluated by histopathology and 1 case was evaluated by electron microscopy. The major histopathologic finding was necrotizing and histiocytic polioencephalomyelitis and ganglionitis. Bacteria formed intracytoplasmic inclusions within neurons but frequently extended into the surrounding tissue from necrotic cells. Ultrastructural evaluation showed the bacteria formed reticulate and elementary bodies characteristic of Chlamydia spp.


Assuntos
Bufonidae/microbiologia , Infecções por Chlamydophila/veterinária , Chlamydophila pneumoniae , Encefalomielite/veterinária , Animais , Animais de Zoológico , Infecções por Chlamydophila/microbiologia , Encefalomielite/microbiologia
3.
APMIS ; 127(3): 131-138, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30746791

RESUMO

Obligate intracellular bacterium Chlamydia pneumoniae causes respiratory tract infections such as community-acquired pneumonia. During infection, C. pneumoniae induces inflammatory responses in host cells and the oxygen concentration at the infection sites subsequently decreases. Because hypoxic conditions influence further inflammatory responses and reduced antibacterial effects, this may exacerbate the C. pneumoniae infection. Here, we show inflammatory responses and drug sensitivity in C. pneumoniae-infected cells under hypoxic conditions. First, we confirmed the enhanced growth of C. pneumoniae under hypoxia, which indicates that the hypoxic condition we used could adequately reproduce past reports. We then demonstrated a significant increase in production of the pro-inflammatory cytokine Interleukin 8 (IL-8) in C. pneumoniae-infected cells under hypoxic conditions. Furthermore, hypoxia decreased the antibacterial effects of azithromycin against C. pneumoniae compared with normoxic conditions. Together, our data suggest that inflammatory responses and drug sensitivity may have been underestimated in C. pneumoniae infection in previous studies. Thus, to accurately understand the Chlamydia infection, it may be necessary to perform in vitro experiments under hypoxic conditions.


Assuntos
Azitromicina/farmacologia , Hipóxia Celular/imunologia , Infecções por Chlamydophila , Chlamydophila pneumoniae/efeitos dos fármacos , Chlamydophila pneumoniae/fisiologia , Interações Hospedeiro-Patógeno , Interleucina-8/metabolismo , Antibacterianos/farmacologia , Linhagem Celular , Infecções por Chlamydophila/imunologia , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/crescimento & desenvolvimento , Farmacorresistência Bacteriana/fisiologia , Humanos , Testes de Sensibilidade Microbiana
4.
J Int Med Res ; 47(2): 635-640, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30392431

RESUMO

OBJECTIVE: Osteoarthritis (OA) is a common cause of disability affecting millions of people of all ages worldwide. The pathogenesis involves an inflammatory component, but the cause of the inflammation remains incompletely understood. The intracellular bacteria Chlamydia trachomatis and C. pneumoniae have been demonstrated in patients with reactive arthritis. Both of these microorganisms can cause chronic and persistent infections, with C. trachomatis being the most common cause of reactive arthritis. This study was performed to investigate the presence of C. pneumoniae in a large number of patients with primary OA. METHODS: The study included 75 patients who underwent total knee arthroplasty. During surgery, a synovial biopsy was performed and synovial fluid drawn. Real-time polymerase chain reaction (PCR) of C. pneumoniae was run on all patients, and real-time PCR of bacterial 16S rDNA was conducted on 30 of the 75 patients to screen for the presence of other bacteria. RESULTS: Real-time PCR showed no evidence of the presence of C. pneumoniae in the patients' specimens, nor were other bacteria detected. CONCLUSIONS: Although an inflammatory component is part of the pathogenesis of OA, we found no evidence indicating that C. pneumoniae is a stimulator of that inflammation.


Assuntos
Infecções por Chlamydophila/complicações , Chlamydophila pneumoniae/isolamento & purificação , Osteoartrite/microbiologia , Líquido Sinovial/microbiologia , Membrana Sinovial/microbiologia , Sinovite/diagnóstico , Idoso , Artroplastia , Infecções por Chlamydophila/microbiologia , DNA Bacteriano/genética , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite/genética , Osteoartrite/cirurgia , Reação em Cadeia da Polimerase , Prognóstico , RNA Ribossômico 16S/genética , Sinovite/genética , Sinovite/microbiologia
5.
Microbes Infect ; 21(2): 104-108, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30292879

RESUMO

The host immune responses that mediate Chlamydia-induced chronic disease sequelae are incompletely understood. The role of TNF-α, TNF receptor 1 (TNFR1), and TNF receptor 2 (TNFR2), in Chlamydia pneumoniae (CPN)-induced atherosclerosis was studied using the high-fat diet-fed male C57BL/6J mouse model. Following intranasal CPN infection, TNF-α knockout (KO), TNFR1 KO, TNFR2 KO, and TNFR 1/2 double-knockout, displayed comparable serum anti-chlamydial antibody response, splenic antigen-specific cytokine response, and serum cholesterol profiles compared to wild type (WT) animals. However, atherosclerotic pathology in each CPN-infected KO mouse group was reduced significantly compared to WT mice, suggesting that both TNFR1 and TNFR2 promote CPN-induced atherosclerosis.


Assuntos
Aterosclerose/imunologia , Infecções por Chlamydophila/imunologia , Chlamydophila pneumoniae/imunologia , Receptores Tipo II do Fator de Necrose Tumoral/metabolismo , Receptores Tipo I de Fatores de Necrose Tumoral/metabolismo , Administração Intranasal , Animais , Anticorpos Antibacterianos/sangue , Aterosclerose/microbiologia , Aterosclerose/patologia , Infecções por Chlamydophila/microbiologia , Infecções por Chlamydophila/patologia , Colesterol/sangue , Citocinas/metabolismo , Dieta Hiperlipídica , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptores Tipo II do Fator de Necrose Tumoral/genética , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
6.
Vet Microbiol ; 225: 31-33, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30322529

RESUMO

Chlamydia abortus is responsible for enzootic abortion (known as ovine enzootic abortion (OEA) and enzootic abortion of ewes (EAE)) in both sheep and goats and has major economic implications for the farming industry worldwide. A virulence-attenuated mutant strain of C. abortus (strain 1B) is currently commercially available as a live attenuated vaccine for immunization of sheep and goats in several European countries. Following an abortion storm in a French flock of 200 ewes that occurred two years after vaccination of 36 replacement ewes with the commercial 1B vaccine strain, the vaginal swabs of 3 vaccinated and 7 unvaccinated aborted ewes and 12 of the 13 dead fetuses were found to be positive for C. abortus by real-time PCR. Genotyping of the samples, using vaccine-specific SNP markers, identified all as positive for the vaccine-type strain. The recent vaccination of this flock with the attenuated commercial vaccine strain, the large number of abortion cases observed in ewes irrespective of vaccination status, the high C. abortus load detected in vaginal swabs or abortion tissues and the identification of specific vaccine-type markers in these samples strongly suggest that the 1B strain has been transmitted from vaccinated to naïve animals, thus mimicking a natural wild-type infection.


Assuntos
Feto Abortado/microbiologia , Aborto Animal/epidemiologia , Vacinas Bacterianas/efeitos adversos , Infecções por Chlamydophila/veterinária , Vacinação/efeitos adversos , Aborto Animal/microbiologia , Aborto Animal/prevenção & controle , Animais , Vacinas Bacterianas/administração & dosagem , Chlamydophila/genética , Infecções por Chlamydophila/microbiologia , Infecções por Chlamydophila/mortalidade , Infecções por Chlamydophila/prevenção & controle , Feminino , França/epidemiologia , Mutação , Reação em Cadeia da Polimerase , Gravidez , Reação em Cadeia da Polimerase em Tempo Real , Ovinos/imunologia , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/prevenção & controle , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/efeitos adversos , Vacinas Atenuadas/imunologia , Vagina/microbiologia , Sequenciamento Completo do Genoma
7.
J Med Microbiol ; 67(9): 1410-1415, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30074473

RESUMO

PURPOSE: The antimicrobial activity of N-chlorotaurine (NCT), an endogenous long-lived oxidant applied topically, was tested against Chlamydiae in vitro. METHODOLOGY: Elementary bodies of Chlamydia pneumoniae strain CV-6 and Chlamydia trachomatis serovars A and D were incubated in 0.01, 0.1 and 1 % (w/v) NCT solution at pH 7.1 and 37 °C. After different incubation times, aliquots were removed and grown in cell culture. The number of inclusion forming units was quantified by immunofluorescence and real-time qPCR.Results/Key findings.Chlamydia pneumoniae and Chlamydia trachomatis were inactivated by 1 and 0.1 % NCT within 1 min. Moreover, 0.025-0.1 % NCT significantly reduced the number of intracellularly growing C. pneumoniae within 30 min. CONCLUSIONS: This is the first study demonstrating the antimicrobial activity of NCT against Chlamydiae. Clinical implications of these findings have to be investigated in further trials.


Assuntos
Anti-Infecciosos Locais/farmacologia , Chlamydia trachomatis/efeitos dos fármacos , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/efeitos dos fármacos , Taurina/análogos & derivados , Infecções por Chlamydia , Chlamydia trachomatis/crescimento & desenvolvimento , Chlamydophila pneumoniae/genética , Chlamydophila pneumoniae/crescimento & desenvolvimento , Chlamydophila pneumoniae/metabolismo , Humanos , Taurina/farmacologia
8.
J Gen Appl Microbiol ; 64(5): 253-257, 2018 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-29760350

RESUMO

Chlamydia pneumoniae is an obligate intracellular pathogen responsible for respiratory diseases, including pneumonia and bronchitis, and is highly involved in chronic diseases, including atherosclerosis, asthma, and Alzheimer's disease. We previously showed that the host apoptotic factor caspase-9 played a crucial role for chlamydial multiplication and host apoptosis inhibition by chlamydial infection. To identify chlamydial genes interacting with human caspase-9, yeast two-hybrid screening was performed and 5 chlamydial genes, including Cpj0838 and pmpG were isolated from the C. pneumoniae genomic library. Pull-down experiments showed that caspase-9 physically bound to the Cpj0838 product and chlamydial cells, which contain PmpG proteins. This study could provide a clue to understanding host-Chlamydia interactions, especially the apoptosis repression by Chlamydia infection.


Assuntos
Apoptose , Proteínas da Membrana Bacteriana Externa/metabolismo , Caspase 9/metabolismo , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/genética , Proteínas da Membrana Bacteriana Externa/genética , Chlamydophila pneumoniae/metabolismo , Interações Hospedeiro-Patógeno , Humanos , Técnicas do Sistema de Duplo-Híbrido
9.
Biochem Biophys Res Commun ; 497(2): 742-748, 2018 03 04.
Artigo em Inglês | MEDLINE | ID: mdl-29462613

RESUMO

Migration of monocytes into the subendothelial layer of the intima is one of the critical events in early atherosclerosis. Chlamydia pneumoniae (C. pneumoniae) infection has been shown to promote monocyte transendothelial migration (TEM). However, the exact mechanisms have not yet been fully clarified. In this study, we tested the hypothesis that C. pneumoniae infection increases vascular endothelial cell (VEC) permeability and subsequent monocyte TEM through stimulating the tyrosine phosphorylation of vascular endothelial-cadherin (VE-cadherin). Here, we demonstrated that C. pneumoniae infection promoted monocyte TEM in a TEM assay possibly by increasing the permeability of a VEC line EA.hy926 cell as assessed by measuring the passage of FITC-BSA across a VEC monolayer. Subsequently, Western blot analysis showed that C. pneumoniae infection induced VE-cadherin internalization. Our further data revealed that Src-mediated VE-cadherin phosphorylation at Tyr658 was involved in C. pneumoniae infection-induced internalization of VE-cadherin, VEC hyperpermeability and monocyte TEM. Taken together, our data indicate that C. pneumoniae infection promotes monocyte TEM by increasing VEC permeability via the tyrosine phosphorylation and internalization of VE-cadherin in VECs.


Assuntos
Antígenos CD/metabolismo , Caderinas/metabolismo , Permeabilidade Capilar , Infecções por Chlamydophila/metabolismo , Chlamydophila pneumoniae/fisiologia , Monócitos/microbiologia , Migração Transendotelial e Transepitelial , Células Cultivadas , Infecções por Chlamydophila/microbiologia , Infecções por Chlamydophila/patologia , Endotélio Vascular/metabolismo , Endotélio Vascular/microbiologia , Endotélio Vascular/patologia , Interações Hospedeiro-Patógeno , Humanos , Monócitos/citologia , Monócitos/patologia , Fosforilação
11.
Respir Med ; 132: 122-131, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29229084

RESUMO

INTRODUCTION: Hajj pilgrimage is the biggest and longest mass gathering in the Muslim world. Annually, about 50% of more than 2.5 million pilgrims participating in this ritual get involved in severe devastating coughs. Most coughs continue, so the pilgrims turn back home and transmit them to family members and other people. Despite the high prevalence of coughs for many years, what causes them remains unknown. Considering the pertussis-like clinical picture of the so-called "hajj coughs", the researchers conducted a study to measure antibodies against three known common atypical bacteria, namely Bordetella Pertussis, Chlamydia Pneumonia and Mycoplasma Pneumonia. PATIENTS AND METHODS: The study was done on three out of eleven groups of pilgrims from Yazd province, central Iran. The sample was selected randomly and consisted of 202 pilgrims who completed an informed consent. Their blood samples were taken, and the plasma was separated and then stored at -70 °C. After turning back from the journey, the pilgrims had their second blood samples taken. As many as 52 pilgrims failed to come for the second sampling, and two samples were broken during transportation. The final analysis was performed on the remaining 148 pairs of samples. RESULTS: Antibodies were already elevated in many pilgrims before the journey probably due to their old age (causing more exposure to pathogens) or unplanned pertussis vaccination. After their return, antibody elevation was only mild, again probably due to the old age of the participants (i.e. due to their weaker immune systems). Some antibodies even fell down without any known reason. In this study, previous hajj journey was assumed as a prophylactic factor, due to acquisition of immunity. Coughs with a prolonged pertussis-like picture were also presumed to be more related than other types of coughs to atypical pathogens. Statistical tests showed that the history of previous journeys had no prophylactic effect. Also, no correlation was found between the clinical pictures of coughs and infection with atypical bacteria. CONCLUSION: Even though some rises and falls occurred in the antibodies titer, the variations could hardly be attributed to coughs in this study. Indeed, the variation of antibodies had no meaningful relationship with clinical factors. In this regard, further studies are needed to clarify the reason for the so-called "hajj coughs", but epidemiological studies will be difficult to do until easier and more reliable methods become available for accurate diagnosis.


Assuntos
Infecções por Chlamydophila/epidemiologia , Tosse/epidemiologia , Islamismo , Pneumonia por Mycoplasma/epidemiologia , Viagem , Coqueluche/epidemiologia , Adulto , Fatores Etários , Anticorpos Antibacterianos/imunologia , Bordetella pertussis/imunologia , Infecções por Chlamydophila/imunologia , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/imunologia , Coinfecção/epidemiologia , Coinfecção/imunologia , Coinfecção/microbiologia , Tosse/imunologia , Tosse/microbiologia , Feminino , Seguimentos , Humanos , Irã (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Mycoplasma pneumoniae/imunologia , Vacina contra Coqueluche/uso terapêutico , Pneumonia por Mycoplasma/imunologia , Pneumonia por Mycoplasma/microbiologia , Estudos Soroepidemiológicos , Coqueluche/imunologia , Coqueluche/microbiologia , Coqueluche/prevenção & controle
12.
Biomed Res Int ; 2017: 3120138, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29057257

RESUMO

OBJECTIVE: To understand the prevalence and distribution of Chlamydia pneumoniae (CP) and Mycoplasma pneumoniae (MP) in the population and to provide a basis for the prevention and treatment of respiratory tract infection. METHODS: This study included a total of 4500 healthy subjects who were given physical examination in Shenzhen People's Hospital from January to December in 2016. Venous blood was drawn from people to detect the MP- and CP-specific IgG and IgM in the serum using chemiluminescence immunoassay (CLIA). The relationship of MP and CP infections with patient age, seasons, and percentage of infections was analyzed. CONCLUSION: CP and MP cause high rate of asymptomatic infection, which may be associated with the high incidence of CP and MP infection, especially in children and the elderly population. Therefore, the implementation of effective and practical prevention measures has become an urgent need. MP culture and drug sensitivity test should be performed as early as possible in patients with manifested MP infections in order to ensure timely and proper treatment and to reduce the emergence of drug-resistant strains.


Assuntos
Infecções por Chlamydophila/sangue , Chlamydophila pneumoniae/isolamento & purificação , Mycoplasma pneumoniae/isolamento & purificação , Pneumonia por Mycoplasma/sangue , Adolescente , Adulto , Idoso , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/isolamento & purificação , Criança , Pré-Escolar , China/epidemiologia , Infecções por Chlamydophila/tratamento farmacológico , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/efeitos dos fármacos , Chlamydophila pneumoniae/patogenicidade , Farmacorresistência Bacteriana/genética , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mycoplasma pneumoniae/efeitos dos fármacos , Mycoplasma pneumoniae/patogenicidade , Pneumonia por Mycoplasma/tratamento farmacológico , Pneumonia por Mycoplasma/microbiologia , Infecções Respiratórias/sangue , Infecções Respiratórias/tratamento farmacológico , Infecções Respiratórias/microbiologia , Testes Sorológicos
13.
Epidemiol Infect ; 145(14): 3076-3084, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28879822

RESUMO

Several infections have been linked to telomere shortening and in some cases these associations have varied by sex. We assessed the association between seropositivity to four persistent pathogens (cytomegalovirus (CMV), herpes simplex virus-1, Helicobacter pylori, Chlamydia pneumoniae), and total pathogen burden on leukocyte telomere length in a diverse US sample. Data came from the Multi-Ethnic Study of Atherosclerosis, a population-based cohort study. We utilized cross-sectional survey data, and biological samples from participants tested for pathogens and telomere length (N = 163). Linear regression was used to examine the association between seropositivity for individual pathogens as well as total pathogen burden and telomere length, adjusting for various confounders. CMV seropositivity and increased total pathogen burden level were significantly associated with shorter telomere length among females (ß = -0·1204 (standard error (s.e.) 0·06), P = 0·044) and (ß = -0·1057 (s.e. = 0·05), P = 0·033), respectively. There was no statistically significant association among males. Our findings suggest that prevention or treatment of persistent pathogens, in particular CMV, may play an important role in reducing telomere shortening over the life course among women. Future research is needed to confirm these novel findings in larger longitudinal samples.


Assuntos
Carga Bacteriana , Leucócitos/fisiologia , Encurtamento do Telômero , Carga Viral , Idoso , Idoso de 80 Anos ou mais , Aterosclerose/etiologia , Infecções por Chlamydophila/epidemiologia , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/fisiologia , Citomegalovirus/fisiologia , Infecções por Citomegalovirus/epidemiologia , Infecções por Citomegalovirus/virologia , Feminino , Infecções por Helicobacter/epidemiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/fisiologia , Herpes Simples/epidemiologia , Herpes Simples/virologia , Herpesvirus Humano 1/fisiologia , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Estudos Soroepidemiológicos , Estados Unidos/epidemiologia
14.
PLoS One ; 12(7): e0180551, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28678871

RESUMO

Following infection, the balance between protective immunity and immunopathology often depends on the initial infectious load. Several studies have investigated the effect of infectious dose; however, the mechanism by which infectious dose affects disease outcomes and the development of a protective immune response is not known. The aim of this study was to investigate how the infectious dose modulates the local and systemic humoral and the cellular immune responses during primary ocular chlamydial infection in the guinea pig animal model. Guinea pigs were infected by ocular instillation of a Chlamydophila caviae-containing eye solution in the conjunctival sac in three different doses: 1×102, 1×104, and 1×106 inclusion forming units (IFUs). Ocular pathology, chlamydial clearance, local and systemic C. caviae-specific humoral and cellular immune responses were assessed. All inocula of C. caviae significantly enhanced the local production of C. caviae-specific IgA in tears, but only guinea pigs infected with the higher doses showed significant changes in C. caviae-specific IgA levels in vaginal washes and serum. On complete resolution of infection, the low dose of C. caviae did not alter the ratio of CD4+ and CD8+ cells within guinea pigs' submandibular lymph node (SMLN) lymphocytes while the higher doses increased the percentages of CD4+ and CD8+ cells within the SMLN lymphocytes. A significant negative correlation between pathology intensity and the percentage of CD4+ and CD8+ cells within SMLN lymphocyte pool at selected time points post-infection was recorded for both 1×104, and 1×106 IFU infected guinea pigs. The relevance of the observed dose-dependent differences on the immune response should be further investigated in repeated ocular chlamydial infections.


Assuntos
Infecções por Chlamydophila/imunologia , Infecções por Chlamydophila/microbiologia , Chlamydophila , Imunidade Celular , Imunidade Humoral , Animais , Chlamydophila/patogenicidade , Relação Dose-Resposta Imunológica , Infecções Oculares , Cobaias , Virulência/imunologia
15.
Biomed Res Int ; 2017: 3642301, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28691023

RESUMO

Chlamydia pneumoniae (C. pneumoniae) may be a mediator in the pathogenesis of atherosclerosis. For its growth C. pneumoniae depends on iron (Fe), but how Fe changes in tissues during persistent infection or affects bacterial replication in tissues is unknown. C. pneumoniae-infected C57BL/6J mice were sacrificed on days 4, 8, 20, and 40. Mice had bacteria in the lungs and liver on all days. Inflammatory markers, chemokine Cxcl2 and interferon-gamma, were not affected in the liver on day 40. The copper (Cu)/zinc (Zn) ratio in serum, another marker of infection/inflammation, increased on day 4 and tended to increase again on day 40. The Fe markers, transferrin receptor (TfR), Hepcidin (Hamp1), and ferroportin 1 (Fpn1), increased in the liver on day 4 and then normalized except for TfR that tended to decrease. TfR responses were similar to Fe in serum that increased on day 4 but tended to decrease thereafter. In the liver, Fe was increased on day 4 and also on day 40. The reappearing increases in Cu/Zn on day 40 concomitant with the increase in liver Fe on day 40, even though TfR tended to decrease, and the fact that viable C. pneumoniae was present in the lungs and liver may indicate the early phase of activation of recurrent infection.


Assuntos
Infecções por Chlamydophila/metabolismo , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/fisiologia , Homeostase , Ferro/metabolismo , Especificidade de Órgãos , Animais , Biomarcadores/metabolismo , Peso Corporal , Quimiocinas/genética , Quimiocinas/metabolismo , Feminino , Regulação da Expressão Gênica , Inflamação/patologia , Fígado/metabolismo , Fígado/microbiologia , Fígado/patologia , Pulmão/metabolismo , Pulmão/microbiologia , Pulmão/patologia , Camundongos Endogâmicos C57BL , Oligoelementos/sangue , Oligoelementos/metabolismo
16.
BMC Microbiol ; 17(1): 153, 2017 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-28693414

RESUMO

BACKGROUND: Chlamydia pneumoniae (C. pneumoniae) is pathogenic to humans, by causing pulmonary inflammation or bronchitis in both adolescents and young adults. However, the molecular signals linking C. pneumoniae components to inflammation remain elusive. This study was to investigate the effect of Chlamydia-specific Cpn0423 of C. pneumoniae on C. pneumoniae-mediated inflammation. RESULTS: Cpn0423 was detected outside of C. pneumoniae inclusions, which induced production of several cytokines including macrophage inflammatory protein-2 (MIP-2) and interleukins (ILs). Production of the Cpn0423-induced cytokines was markedly reduced in cells pretreated with NOD2-siRNA, but not with negative control oligonucleotides. Mice treated with Cpn0423 through intranasal administration exhibited pulmonary inflammation as evidenced by infiltration of inflammatory cells, increased inflammatory scores in the lung histology, recruitment of neutrophils and increased cytokines levels in the BALF. CONCLUSION: Cpn0423 could be sensed by NOD2, which was identified as an essential element in a pathway contributing to the development of C. pneumoniae -mediated inflammation.


Assuntos
Proteínas de Bactérias/imunologia , Infecções por Chlamydophila/imunologia , Chlamydophila pneumoniae/imunologia , Mediadores da Inflamação/imunologia , Proteína Adaptadora de Sinalização NOD2/imunologia , Pneumonia Bacteriana/microbiologia , Animais , Proteínas de Bactérias/genética , Quimiocina CXCL2/genética , Quimiocina CXCL2/imunologia , Infecções por Chlamydophila/genética , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/genética , Humanos , Interleucinas/genética , Interleucinas/imunologia , Pulmão/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Proteína Adaptadora de Sinalização NOD2/genética , Pneumonia Bacteriana/genética , Pneumonia Bacteriana/imunologia
17.
Methods Mol Biol ; 1616: 171-181, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28600769

RESUMO

The advances in molecular biology of the last decades have dramatically improved the field of diagnostic bacteriology. In particular, PCR-based technologies have impacted the diagnosis of infections caused by obligate intracellular bacteria such as pathogens from the Chlamydiacae family. Here, we describe a real-time PCR-based method using the Taqman technology for the diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus infection. The method presented here can be applied to various clinical samples and can be adapted on opened molecular diagnostic platforms.


Assuntos
Infecções por Chlamydia/diagnóstico , Infecções por Chlamydophila/diagnóstico por imagem , Reação em Cadeia da Polimerase em Tempo Real/métodos , Animais , Infecções por Chlamydia/microbiologia , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/patogenicidade , Chlamydophila psittaci/patogenicidade , Humanos , Técnicas de Diagnóstico Molecular/instrumentação , Técnicas de Diagnóstico Molecular/métodos , Psitacose/diagnóstico , Psitacose/microbiologia
18.
Int J Med Microbiol ; 307(4-5): 276-286, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28377051

RESUMO

Chlamydia pneumoniae (C. pneumoniae) infection plays a potential role in angiogenesis. However, it is still an enigma how C. pneumoniae is involved in this process. Therefore, we investigated the effect of C. pneumoniae infection on angiogenesis, and then explored the roles of IQGAP1-related signaling in C. pneumoniae infection-induced angiogenesis. C. pneumoniae infection significantly enhanced angiogenesis as assessed by the tube formation assay possibly by inducing vascular endothelial cell (VEC) migration in the wound healing and Transwell migration assays. Subsequently, immunoprecipitation, Western blot and tube formation assay results showed that the phosphorylation of both IQGAP1 and N-WASP was required for the angiogenesis induced by C. pneumoniae infection. Our co-immunoprecipitation study revealed that IQGAP1 physically associated with N-WASP after C. pneumoniae infection of VECs. Actin polymerization assay further showed that in C. pneumoniae-infected VECs, both IQGAP1 and N-WASP were recruited to filamentous actin, and shared some common compartments localized at the leading edge of lamellipodia, which was impaired after the depletion of IQGAP1 by using the small interference RNA. Moreover, the knockdown of IQGAP1 also significantly decreased N-WASP phosphorylation at Tyr256 induced by C. pneumoniae infection. We conclude that C. pneumoniae infection promotes VEC migration and angiogenesis presumably through the IQGAP1-related signaling pathway.


Assuntos
Células Endoteliais/citologia , Neovascularização Patológica/microbiologia , Transdução de Sinais , Proteínas Ativadoras de ras GTPase/metabolismo , Actinas/genética , Actinas/metabolismo , Linhagem Celular , Movimento Celular , Infecções por Chlamydophila/complicações , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae , Células Endoteliais/microbiologia , Humanos , Fosforilação , Proteína Quinase C/metabolismo , RNA Interferente Pequeno/genética , Proteína Neuronal da Síndrome de Wiskott-Aldrich/metabolismo , Quinases da Família src/metabolismo
19.
Pathog Dis ; 75(4)2017 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-28387800

RESUMO

Chlamydia pneumoniae is a respiratory pathogen associated with chronic inflammatory diseases such as asthma and atherosclerosis, and its detection in human carotid and coronary atheroma suggests some support for its involvement in atherogenesis. The main objective of our study was to evaluate the association between Chlamydia pneumoniae and atherosclerosis in Moroccan patients through a case-control approach and detected strain genotyping. A total of 137 cases and 124 controls were enrolled, nested PCR was performed for Chlamydia pneumoniae screening of the peripheral blood mononuclear cells (PBMCs) of both cases and controls as well as atheroma plaques from 37 cases, and positive samples were subjected to sequencing for genotyping and phylogenetic analysis. The results showed 54% and 18%, respectively, for positivity in cases and control PBMCs and 86.5% in atheroma plaques, the difference being significant between the two groups (P < 0.001, ORa = 8.580, CI, 95% [3.273-22.491]). Strain sequence analyses showed more than 98% similarity with human reference strains, and revealed various genotypes. This study supports the involvement of Chlamydia pneumoniae in atherosclerosis in the studied population and genotyping revealed that detected strains were identical to human strains circulating worldwide.


Assuntos
Aterosclerose/microbiologia , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/genética , DNA Bacteriano/genética , Filogenia , Placa Aterosclerótica/microbiologia , Adulto , Idoso , Aterosclerose/patologia , Técnicas de Tipagem Bacteriana , Sequência de Bases , Estudos de Casos e Controles , Infecções por Chlamydophila/patologia , Chlamydophila pneumoniae/classificação , Chlamydophila pneumoniae/isolamento & purificação , Feminino , Humanos , Leucócitos Mononucleares/metabolismo , Leucócitos Mononucleares/microbiologia , Masculino , Pessoa de Meia-Idade , Marrocos , Placa Aterosclerótica/patologia , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico
20.
Pathog Dis ; 75(1)2017 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-28158541

RESUMO

Chlamydia pneumoniae has been suggested as a stimulator of the atherosclerotic process. Mice fed a normal diet were infected intranasally with C. pneumoniae and given one intraperitoneal injection of 14C-cholesterol tracer per day for 12 days. Bacteria were demonstrated in the aorta in the early phase of infection and in lungs and liver throughout the study period of 20 days. 14C-cholesterol was not affected in the heart but increased in the blood, liver and aorta on day 4 when the infection was clinically most severe. Furthermore, on day 20 14C-cholesterol tended to be increased in the aorta. Accordingly, copper- and zinc levels and expressions of the infection biomarkers Cxcl2 and Ifng increased in the liver on day 4 with a tendency of increased of copper, zinc and Ifng on day 20. In mice where bacteria could be cultivated from the lungs, expressions of cholesterol transporters Abca1 and Idol were both increased in the liver on day 4. The increased levels of 14C-cholesterol in blood and aorta together with increased Abca1 and Idol in the liver during C. pneumoniae infection in mice fed a normal diet suggest that this pathogen may have a role in the initiation of the atherosclerotic process.


Assuntos
Aorta/metabolismo , Aorta/patologia , Infecções por Chlamydophila/metabolismo , Infecções por Chlamydophila/patologia , Chlamydophila pneumoniae , Colesterol/metabolismo , Transportador 1 de Cassete de Ligação de ATP/genética , Transportador 1 de Cassete de Ligação de ATP/metabolismo , Animais , Aorta/microbiologia , Transporte Biológico , Biomarcadores , Infecções por Chlamydophila/genética , Infecções por Chlamydophila/microbiologia , Chlamydophila pneumoniae/genética , Chlamydophila pneumoniae/imunologia , Cobre/metabolismo , Feminino , Interações Hospedeiro-Patógeno/genética , Interações Hospedeiro-Patógeno/imunologia , Mediadores da Inflamação/metabolismo , Fígado/imunologia , Fígado/metabolismo , Fígado/microbiologia , Fígado/patologia , Camundongos , Zinco/metabolismo
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