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1.
Ter Arkh ; 92(7): 100-103, 2020 Sep 01.
Artigo em Russo | MEDLINE | ID: mdl-33346452

RESUMO

Here we provide a review of the literature and a description of our own clinical case. The patient was a 32-year-old woman who had been infected with HIV for 6 years without antiretroviral therapy. The test results showed CD4 87 cells/l, viral load 3750 copies/ml. Normochromic normocytic anemia and reticulocytopenia developed soon. In the myelogram, all erythroblasts were 0.5%. The viral load of parvovirus B19 DNA according to PCR was more than 9 million IU/ml. Pure red cell aplasia associated with parvovirus B19 was diagnosed. We started antiretroviral therapy with efavirenz, lamevudine and tenofovir. In addition to blood transfusions, we administered intravenous donor immunoglobulin with a dose increase from 5000 mg to 20 000 mg per day. After discontinuing of intravenous immunoglobulins, the laboratory test results were stable over the next 5 months: hemoglobin was more than 115 g/L, reticulocytes more than 3%, in the myelogram all erythroblasts were 21%. However, the elimination of parvovirus B19 wasnt achieved. The maximum decrease in viral load for parvovirus B19 was down to 720 IU/ml. A typical feature of the case was the lack of pure red cell aplasia of the bone marrow with the existing viral load of parvovirus B19. HIV infection progressed: 44 cells/l, viral load not determined. The case ended lethally.


Assuntos
Eritema Infeccioso , Infecções por HIV , Infecções por Parvoviridae , Parvovirus B19 Humano , Aplasia Pura de Série Vermelha , Adulto , Feminino , Infecções por HIV/complicações , Infecções por HIV/diagnóstico , Infecções por HIV/tratamento farmacológico , Humanos , Imunoglobulinas Intravenosas , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/diagnóstico
2.
Medicine (Baltimore) ; 99(36): e22079, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32899080

RESUMO

RATIONALE: Parvovirus B19 has been linked to polyarteritis nodosa (PAN), but there is some controversy about its pathogenesis regarding whether it is triggered by the immune complex or by the activated immune cells that phagocytose viruses. PATIENT CONCERNS: A 38-year-old woman was admitted with fever and bicytopenia. She also complained of a painful palpable nodule in the left forearm. DIAGNOSIS: Her bone marrow aspirate revealed erythroblasts in abnormal megaloblastic changes, some of which presented with pseudopods, and parvovirus B19 was positive in a PCR analysis of her blood, which was compatible with parvovirus B19-induced hemophagocytic syndrome. Skin excisional biopsy of the nodule on the left forearm revealed a heavy inflammatory cell infiltrate throughout whole layers of a medium-sized vessel, the characteristic feature of PAN. PCR analysis of the vasculitis tissue showed a positive result for parvovirus B19. INTERVENTIONS: Her symptoms spontaneously resolved with supportive care. OUTCOMES: She underwent regular follow-up without recurrence of vasculitis-associated symptoms. LESSONS: This case highlights the presence of parvovirus B19 DNA in vasculitis tissues, which can support the role of cellular immune response in the pathogenesis of parvovirus-associated PAN.


Assuntos
Linfo-Histiocitose Hemofagocítica/complicações , Linfo-Histiocitose Hemofagocítica/virologia , Infecções por Parvoviridae/complicações , Poliarterite Nodosa/complicações , Poliarterite Nodosa/virologia , Adulto , DNA Viral , Feminino , Humanos , Parvovirus B19 Humano
4.
Vet Microbiol ; 242: 108590, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32122594

RESUMO

Recombinant Muscovy duck parvovirus (rMDPV) has been recently identified as a novel pathogen circulating in Chinese Muscovy duck flocks in the past two decades. Different from classical MDPV, rMDPV infection can form embolism in the intestinal tract of deceased Muscovy ducklings. However, whether rMDPV acts as the sole causative agent involved in the formation of the characteristic embolism in Muscovy ducklings remains unclear. In this study, an infectious plasmid clone pZW containing the complete genome of strain ZW, a previously characterized rMDPV isolate, was constructed, and a single nucleotide mutation was then introduced in the VP1 gene within pZW as the genetic marker. Transfection of pZW in 11-day-old embryonated Muscovy duck eggs via the chorioallantoic membrane route resulted in the rescue of the infectious virus. The rescued virus exhibited similar biological characteristics to its parental strain ZW, as evaluated by the median embryo lethal dose and the replication kinetics in embryonated Muscovy duck eggs. Muscovy duckling infection tests showed that the rescued virus and parental strain can kill all Muscovy ducklings within 7 days post-infection. Postmortem examination revealed that embolism can be observed in the intestinal tracts of deceased ducklings in the rescued and parental virus infection groups. Collectively, the present study demonstrated that sole rMDPV infection of Muscovy ducklings, without participation of other pathogens, is enough to form characteristic embolism in the intestinal tract.


Assuntos
Patos/virologia , Embolia/veterinária , Intestinos/patologia , Infecções por Parvoviridae/veterinária , Parvovirinae/genética , Parvovirinae/patogenicidade , Animais , Embolia/virologia , Genoma Viral , Intestinos/virologia , Cinética , Dose Letal Mediana , Óvulo/virologia , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/mortalidade , Doenças das Aves Domésticas/virologia , Transfecção
5.
BMJ Case Rep ; 13(3)2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32193179

RESUMO

Malignancies are often associated with autoimmune diseases, which are addressed by treating the underlying cancer. However, there are rare malignancies that can cause autoimmune diseases even after appropriate treatment. Our patient is a 39-year-old Hispanic man with a malignant thymoma recently treated with chemotherapy and radiation who presented with syncope and dyspnoea. He was found to be both anaemic and thrombocytopenic. His labs were consistent with autoimmune haemolytic anaemia (AIHA), except his reticulocyte count was unexpectedly low. Bone marrow biopsy supported a diagnosis of Evans syndrome, a rare autoimmune condition characterised by (AIHA) combined with immune thrombocytopenia. He was also found to have an acute parvovirus B19 infection. He was treated with steroids and RBC transfusion. His blood counts gradually returned to baseline, with improvement in symptoms. This patient's thymoma treatment and active parvovirus B19 infection likely both played a role in the development of Evans syndrome.


Assuntos
Anemia Hemolítica Autoimune/etiologia , Infecções por Parvoviridae/complicações , Trombocitopenia/etiologia , Timoma/complicações , Neoplasias do Timo/complicações , Adulto , Anemia Hemolítica Autoimune/terapia , Diagnóstico Diferencial , Transfusão de Eritrócitos , Glucocorticoides/uso terapêutico , Humanos , Masculino , Infecções por Parvoviridae/terapia , Parvovirus B19 Humano , Prednisona/uso terapêutico , Trombocitopenia/terapia , Timoma/terapia
6.
Artigo em Inglês | MEDLINE | ID: mdl-32046345

RESUMO

Human bocavirus (HBoV) is a causative agent of respiratory and gastrointestinal diseases worldwide. Four HBoV species (HBoV1-4) have been identified so far. Although a previous report has documented the HBoV association with acute gastroenteritis (AGE) in Taiwan, their epidemiology, genetic diversity, and phylogenetic relationships remain unclear. In this study, we focused on an investigation of these unsolved issues, which will help to reveal molecular epidemiology and phylogeny of the circulating HBoV2 in Taiwan. A total of 176 stool samples were collected from children with AGE for this study. PCR amplification and sequencing on the VP1 gene region were used to identify species. Phylogenetic analysis was conducted by maximum-likelihood and neighbor-joining methods. Selection pressure was also estimated to obtain HBoV evolutionary information. Our results showed the prevalence of HBoV in AGE children was 8.5%, of which HBoV1 was the predominant species (6.3%), followed by HBoV2 (2.3%). Phylogenetic analysis showed those Taiwanese HBoV2 strains have significant genetic variability and can be divided into two clusters. One belongs to HBoV2 genotype A and the other forms an independent unclassified cluster. The nucleotide distance between that independent cluster and the known HBoV2 genotypes was more than 5%, suggesting a new HBoV2 genotype. No positive selection site was found and the virus was under purifying selection. This is the first report to reveal HBoV2 genetic diversity and phylogenetic relationships among AGE children in Taiwan. We find that HBoV2 may have been introduced into the country by multiple origins, and a potential new HBoV2 genotype is proposed.


Assuntos
Gastroenterite/virologia , Variação Genética , Bocavirus Humano/genética , Infecções por Parvoviridae/virologia , Filogenia , Doença Aguda , Criança , Pré-Escolar , Fezes/virologia , Feminino , Gastroenterite/epidemiologia , Genótipo , Bocavirus Humano/isolamento & purificação , Humanos , Lactente , Masculino , Epidemiologia Molecular , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/epidemiologia , Prevalência , Taiwan/epidemiologia
7.
Artigo em Alemão | MEDLINE | ID: mdl-32059250

RESUMO

Canine parvovirus infection remains to represent a worldwide and commonly occurring infectious disease leading to severe morbidity especially in puppies. The main therapeutic approach is primarily based on symptomatic treatment, especially addressing acute gastrointestinal signs as well as treating and preventing potential sepsis due to bacterial translocation. Besides antibiotic and essential fluid therapy, the use of efficient antiemetic and pain medication is required. In addition, early enteral nutrition should be attempted as this has been shown to be associated with a shorter time to recovery. Modulation of the intestinal microbiome could improve clinical signs and possibly aide in avoiding long-term sequelae such as chronic gastrointestinal disease. Treatment with recombinant feline interferon-omega resulted in a lower mortality and a more rapid improvement of clinical signs in several experimental and clinical studies and thus is considered to be effective.


Assuntos
Doenças do Cão/terapia , Infecções por Parvoviridae/veterinária , Parvovirus Canino , Analgésicos/uso terapêutico , Animais , Antibacterianos/uso terapêutico , Antieméticos/uso terapêutico , Antivirais/uso terapêutico , Doenças do Cão/virologia , Cães , Hidratação , Microbioma Gastrointestinal/efeitos dos fármacos , Microbioma Gastrointestinal/imunologia , Hipoglicemia/terapia , Hipoglicemia/veterinária , Hipoproteinemia/terapia , Hipoproteinemia/veterinária , Fatores Imunológicos/uso terapêutico , Apoio Nutricional , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/terapia , Potássio/administração & dosagem , Choque/tratamento farmacológico , Choque/prevenção & controle , Choque/veterinária
8.
Praxis (Bern 1994) ; 109(1): 42-46, 2020 Jan.
Artigo em Alemão | MEDLINE | ID: mdl-31910759

RESUMO

CME Rheumatology 20: Parvovirus B19-Induced Tenosynovitis? Abstract. In this case presentation we discuss diagnostic algorithms and differential diagnoses in undifferentiated tenosynovitis. We present a case of a patient with chronic tenosynovitis in the 4th extensor tendon compartment. With unremarkable anamnesis and due to normal laboratory results a «seronegative (RF), ACPA (CCP)-negative tenosynovitis without arthritis¼ had been reported. Diagnostic and therapeutic tenosynovectomy was performed. Histologic processing revealed a positive PCR for parvovirus B19. We discuss articular and extraarticular manifestations. A parvovirus B19-associated manifestation in the musculoskeletal system is usually self-limiting. The therapy should be carried out symptomatically. In our patient there was a marked local finding, so that the complete tenosynovectomy followed by a single steroid injection led to a persistent restitutio ad integrum.


Assuntos
Artrite , Infecções por Parvoviridae , Parvovirus B19 Humano , Tenossinovite , Diagnóstico Diferencial , Humanos , Infecções por Parvoviridae/complicações , Reação em Cadeia da Polimerase , Tenossinovite/virologia
10.
J Investig Med High Impact Case Rep ; 7: 2324709619883698, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31635495

RESUMO

Hemophagocytic lymphohistiocytosis (HLH) is a rare and life-threatening condition characterized by widespread inflammation due to massive immune activation and cytokine release. It is of 2 types, primary or familial and secondary or acquired. Diagnosis is made by fulfilling 5 of 8 criteria as determined by the Histiocyte Society. Treatment includes etoposide, dexamethasone, with or without intrathecal methotrexate in the presence of neurologic involvement as well as treating the underlying cause in secondary HLH. We present a case of a 23-year-old female with congenital human immunodeficiency virus (HIV) infection who presents with nonspecific signs and symptoms of cough, fever, leukopenia, and anemia, and a high-serum parvovirus B19 DNA, later diagnosed with HLH and treated with etoposide and dexamethasone. She made clinical improvements and was successfully discharged to home after 26 days of admission.


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/diagnóstico , Linfo-Histiocitose Hemofagocítica/virologia , Infecções por Parvoviridae/complicações , Parvovirus B19 Humano , Síndrome de Imunodeficiência Adquirida/congênito , Dexametasona/administração & dosagem , Dexametasona/uso terapêutico , Quimioterapia Combinada , Etoposídeo/administração & dosagem , Etoposídeo/uso terapêutico , Feminino , Humanos , Linfo-Histiocitose Hemofagocítica/diagnóstico , Linfo-Histiocitose Hemofagocítica/tratamento farmacológico , Linfo-Histiocitose Hemofagocítica/etiologia , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/etiologia , Infecções por Parvoviridae/virologia , Adulto Jovem
11.
Pathog Dis ; 77(5)2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-31603501

RESUMO

H1 parvovirus (H1PV) infection in rats is of concern to the research community as infection may compromise rodent-based experiments. The aim of this study was to evaluate the influence of H1PV infection on rat gut microbiota. Inbred Wistar rats were infected with H1PV by routine gavage and clinical signs were recorded. Gross anatomical and histopathological examination of the gut was performed, as was immune cytokine analysis. The cecal contents were also collected for 16S rRNA sequencing. Gross anatomical examination showed distention of the ileum associated with flatulence after infection, while histopathological examination showed hyperemia and inflammatory cell infiltration in the ileum. Upregulation of the interleukin-6 in sera in H1PV infected rats was also detected. The gut microbiota had been significantly changed in H1PV infected rats: there was a reduction in several bacteria species including probiotic bacteria from the genera Parabacteroides and Butyricicoccus, while others were increased, including those from the genera Methanobrevibacter and Syntrophococcus. Taken together, these results demonstrate that chronic H1PV infection in rats leads to gastrointestinal inflammation with flatulence. The gut microbiota alterations were associated with decreased polymorphisms, reduced abundance of probiotic bacteria and increased abundance of methane-producing bacteria.


Assuntos
Bactérias/classificação , Disbiose/veterinária , Microbioma Gastrointestinal , Infecções por Parvoviridae/veterinária , Parvovirus/crescimento & desenvolvimento , Doenças dos Roedores/patologia , Animais , Bactérias/genética , Análise por Conglomerados , Citocinas/sangue , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Disbiose/patologia , Íleo/patologia , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/patologia , Filogenia , RNA Ribossômico 16S/genética , Ratos Wistar , Doenças dos Roedores/microbiologia , Doenças dos Roedores/virologia , Análise de Sequência de DNA
12.
Medicine (Baltimore) ; 98(35): e16993, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464949

RESUMO

RATIONALE: Parvovirus B19 (PV) infection is usually symptomless and can cause benign, short-lived conditions. Anemia associated with PRCA is the most representative hematologic manifestation, but neutropenia and thrombocytopenia have been rarely reported. PATIENT CONCERNS: Three patients were admitted to the hospital with neutropenia and thrombocytopenia. The accompanying symptoms were fever, myalgia, rash, or arthralgia, and all patients were previously healthy. DIAGNOSIS: Patients were positive for PV PCR and diagnosed with PV infection. Before the diagnosis of PV infection, 2 patients underwent BM study and almost absence of erythroid progenitor cells in BM aspiration were a clue for the PV infection. Other BM findings were hypocellular marrow and a few hemophagocytic histiocytes. INTERVENTIONS: Patients received supportive care with follow-up of CBC. OUTCOMES: All 3 patients spontaneously recovered from neutropenia and thrombocytopenia within 3 weeks without severe complications. LESSONS: The evaluation of PV infection should be considered in situations where there is neutropenia and thrombocytopenia in healthy individuals even without anemia as a differential diagnosis.


Assuntos
Neutropenia/complicações , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/diagnóstico , Trombocitopenia/complicações , Adulto , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Infecções por Parvoviridae/virologia , Parvovirus B19 Humano , Reação em Cadeia da Polimerase
13.
Transplant Proc ; 51(8): 2693-2696, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31351772

RESUMO

Parvovirus B19 (PVB19) has tropism to red blood cell progenitors and can be reactivated after organ transplantation. The aim of study was to describe clinical manifestations, laboratory findings, treatments used, and effectiveness in kidney recipients at Viet Duc hospital. A retrospective descriptive study was performed on 663 kidney recipients who were on regular follow-up from 2000 to 2018. PVB19 was detected by polymerase chain reaction PVB19-DNA. Effectiveness of therapy was assessed by Hemoglobin level. Nine out of 663 kidney recipients (1.4%) were diagnosed with PVB19-associated anemia. Eight of these 9 (89%) were diagnosed within the first 3 months following transplantation. All patients had normoscopic anemia; the average reticulocyte proportion and count were 0.15 ± 0.04% and 0.0039 ± 0.0011T/L, respectively. Graft dysfunction was observed in 4/9 (45%) patients. Treatment included reduction of immunosuppression, intravenous immunoglobulin (IVIG), and blood transfusion. All patients responded well to treatment except 1 (11%), who experienced relapse after using low dose of IVIG. PVB19-associated anemia usually occurred early after transplantation and was associated with very low reticulocyte proportion and count. Actual treatment was effective, but the risk of relapse was present.


Assuntos
Anemia/virologia , Transplante de Rim , Infecções por Parvoviridae , Adulto , Feminino , Humanos , Hospedeiro Imunocomprometido/imunologia , Transplante de Rim/efeitos adversos , Masculino , Pessoa de Meia-Idade , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/imunologia , Parvovirus B19 Humano , Estudos Retrospectivos
14.
Emerg Infect Dis ; 25(8): 1600-16002, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31310208

RESUMO

Cutavirus was previously found in cutaneous melanoma. We detected cutavirus DNA in only 2/185 melanoma biopsies and in 0/52 melanoma metastases from patients in Germany. Viral DNA was localized in the upper epidermal layers. Swab specimens from healthy skin were cutavirus positive for 3.8% (9/237) of immunocompetent and 17.1% (35/205) of HIV-positive men.


Assuntos
Melanoma/epidemiologia , Melanoma/etiologia , Infecções por Parvoviridae/complicações , Parvovirus , Biópsia , DNA Viral , Alemanha/epidemiologia , Humanos , Melanoma/diagnóstico , Estadiamento de Neoplasias , Infecções por Parvoviridae/virologia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/epidemiologia , Neoplasias Cutâneas/etiologia , Carga Viral
15.
PLoS One ; 14(5): e0216799, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31086415

RESUMO

Evidence has indicated that viral infection increases the risk of developing asthma. Although the association of human parvovirus B19 (B19V) or human bocavirus (HBoV) with respiratory diseases has been reported, little is known about the influence of the B19V-VP1u and HBoV-VP1u proteins on the symptoms of asthma. Herein, we investigated the systemic influence of subcutaneously injected B19V-VP1u and HBoV-VP1u recombinant proteins in an OVA-sensitized asthmatic mouse model. A significantly higher Penh ratio and IgE level were detected in the serum, bronchoalveolar lavage fluid (BALF) and the supernatant of a lymphocyte culture from mice treated with HBoV-VP1u or B19V-VP1u than in a lymphocyte culture from OVA-sensitized mice. Significantly higher levels of serum and BALF IgE, total IgG, IgG1, OVA-specific IgE and OVA-specific IgG1 were detected in mice treated with HBoV-VP1u or B19V-VP1u than in OVA-sensitized mice. Conversely, a significantly lower IgG2a level was detected in mice from the HBoV-VP1u or B19V-VP1u groups than in mice from the OVA group. The mice treated with HBoV-VP1u or B19V-VP1u exhibited more significant lung inflammatory indices, including elevated serum and BALF IL-4, IL-5, IL-10 and IL-13 levels; BALF lymphocyte, neutrophil and eosinophil counts, MMP-9 and MMP-2 activity; and the amount of lymphocyte infiltration, relative to those in the control mice or in those sensitized with OVA. These findings demonstrate that the subcutaneous injection of HBoV-VP1u or B19V-VP1u proteins in OVA-sensitized mice result in elevated asthmatic indices and suggest that human parvoviruses may increase the risk of developing airway inflammation in a mouse model of asthma.


Assuntos
Asma/virologia , Proteínas do Capsídeo/imunologia , Bocavirus Humano/imunologia , Infecções por Parvoviridae/complicações , Parvovirus B19 Humano/imunologia , Animais , Asma/etiologia , Asma/imunologia , Proteínas do Capsídeo/química , Modelos Animais de Doenças , Feminino , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Infecções por Parvoviridae/imunologia
16.
J Med Case Rep ; 13(1): 104, 2019 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-31014402

RESUMO

BACKGROUND: There are reports of the familial occurrence of Kawasaki disease but only a few reports described Kawasaki disease in siblings. However, the familial cases were not simultaneous. In these patients the idea of infective agents as trigger must be considered. CASE PRESENTATION: We describe two siblings with atypical presentations of Kawasaki disease; the sister was first diagnosed as having parvovirus infection with anemia and the brother was diagnosed as having myocarditis. The first patient was a 9-month-old Caucasian girl with fever, conjunctivitis, rash, and pharyngitis, and later she had cervical adenopathy, diarrhea and vomiting, leukocytosis, and anemia, which were explained by positive immunoglobulin M against parvovirus. However, coronary artery lesions with aneurysms were documented at day 26 after fever onset. An infusion of intravenous immunoglobulin and high doses of steroids were not efficacious to resolve the coronary lesions. She was treated with anakinra, despite a laboratory test not showing inflammation, with prompt and progressive improvement of coronary lesions. Her 7-year-old Caucasian brother presented vomiting and fever at the same time as she was unwell, which spontaneously resolved after 4 days. Four days later, he again presented with fever with abdominal pain, associated with tachypnea, stasis at the pulmonary bases, tachycardia, gallop rhythm, hypotension, secondary anuria, and hepatomegaly. An echocardiogram revealed a severe hypokinesia, with a severe reduction of the ejection fraction (20%). He had an increase of immunoglobulin M anti-parvovirus, tested for the index case of his sister, confirming the suspicion of viral myocarditis. He received dopamine, dobutamine, furosemide plus steroids, with a progressive increase of the ejection fraction to 50%. However, evaluating his sister's history, the brother showed a myocardial dysfunction secondary to Kawasaki shock syndrome. CONCLUSIONS: We report on familial Kawasaki disease in two siblings which had the same infectious trigger (a documented parvovirus infection). The brother was diagnosed as having post-viral myocarditis. However, in view of the two different and simultaneous evolutions, the girl showed Kawasaki disease with late coronary artery lesions and aneurysms, whereas the brother showed Kawasaki shock syndrome with myocardial dysfunction. We stress the effectiveness of anakinra in non-responder Kawasaki disease and the efficacy on coronary aneurysms.


Assuntos
Aneurisma Coronário/virologia , Fatores Imunológicos/uso terapêutico , Infecções por Parvoviridae/complicações , Parvovirus/isolamento & purificação , Choque/virologia , Irmãos , Cardiotônicos/uso terapêutico , Criança , Aneurisma Coronário/tratamento farmacológico , Aneurisma Coronário/fisiopatologia , Dobutamina/uso terapêutico , Dopamina/uso terapêutico , Ecocardiografia , Feminino , Humanos , Imunossupressores/uso terapêutico , Lactente , Proteína Antagonista do Receptor de Interleucina 1/uso terapêutico , Masculino , Infecções por Parvoviridae/tratamento farmacológico , Infecções por Parvoviridae/fisiopatologia , Choque/fisiopatologia , Volume Sistólico , Resultado do Tratamento
19.
J Vet Intern Med ; 33(1): 251-257, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30520162

RESUMO

BACKGROUND: Three flaviviruses (equine pegivirus [EPgV]; Theiler's disease-associated virus [TDAV]; non-primate hepacivirus [NPHV]) and equine parvovirus (EqPV-H) are present in equine blood products; the TDAV, NPHV, and EqPV-H have been suggested as potential causes of serum hepatitis. OBJECTIVE: To determine the prevalence of these viruses in horses with equine serum hepatitis. ANIMALS: Eighteen horses diagnosed with serum hepatitis, enrolled from US referral hospitals. METHODS: In the prospective case study, liver, serum, or both samples were tested for EPgV, TDAV, NPHV, and EqPV-H by PCR. RESULTS: Both liver tissue and serum were tested for 6 cases, serum only for 8 cases, and liver only for 4 cases. Twelve horses received tetanus antitoxin (TAT) 4-12.7 weeks (median = 8 weeks), 3 horses received commercial equine plasma 6-8.6 weeks, and 3 horses received allogenic stem cells 6.4-7.6 weeks before the onset of hepatic failure. All samples were TDAV negative. Two of 14 serum samples were NPHV-positive. Six of 14 serum samples were EPgV-positive. All liver samples were NPHV-negative and EPgV-negative. EqPV-H was detected in the serum (N = 8), liver (N = 4), or both samples (N = 6) of all 18 cases. The TAT of the same lot number was available for virologic testing in 10 of 12 TAT-associated cases, and all 10 samples were EqPV-H positive. CONCLUSIONS AND CLINICAL IMPORTANCE: We demonstrated EqPV-H in 18 consecutive cases of serum hepatitis. EPgV, TDAV, and NPHV were not consistently present. This information should encourage blood product manufacturers to test for EqPV-H and eliminate EqPV-H-infected horses from their donor herds.


Assuntos
Infecções por Flavivirus/veterinária , Hepatite C/veterinária , Hepatite Viral Animal/virologia , Doenças dos Cavalos/virologia , Infecções por Parvoviridae/veterinária , Animais , Feminino , Flavivirus , Infecções por Flavivirus/complicações , Infecções por Flavivirus/virologia , Hepacivirus , Hepatite C/complicações , Hepatite C/virologia , Hepatite Viral Animal/sangue , Hepatite Viral Animal/patologia , Doenças dos Cavalos/sangue , Doenças dos Cavalos/patologia , Cavalos , Fígado/patologia , Fígado/virologia , Masculino , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/virologia , Parvovirus , Estudos Prospectivos , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Theilovirus
20.
J S Afr Vet Assoc ; 89(0): e1-e6, 2018 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-30551702

RESUMO

Salmonellosis is a disease of major zoonotic importance and canine parvovirus is a potentially fatal cause of canine enteritis with a world-wide distribution. Persistent isolation of Salmonella during routine environmental sampling surveys of a hospital ward, reserved for the treatment of dogs with canine parvovirus infection, prompted investigation into a possible source. We hypothesised that dogs affected by canine parvovirus would have a higher prevalence of faecal salmonellae compared to an apparently healthy cohort. Seventy-four client-owned dogs naturally infected with canine parvovirus and 42 apparently healthy client-owned dogs were included in the study. This prospective, longitudinal, observational study was conducted over an 18-month period. Fresh faecal samples were collected from dogs aged 6 weeks to 9 months diagnosed with canine parvovirus infection and admitted for treatment, and from apparently healthy dogs presented for vaccination or routine hospital procedures. Faeces were submitted for the isolation, antimicrobial susceptibility testing and serotyping of salmonellae. The prevalence of faecal Salmonella shedding was 22% and 31% for the affected and apparently healthy dogs, respectively, which was not statistically different. No significant associations between Salmonella status and possible risk factors or continuous variables such as age, body weight and duration of hospitalisation were identified. All the Salmonella isolates (n = 32) were resistant to penicillin G, lincomycin and tylosin. Salmonellae from nine different serotypes were identified. The prevalence of Salmonella shedding in both groups was higher than that commonly reported, yet similar to those in previous reports on young dogs, shelter dogs or dogs fed a raw meat diet.


Assuntos
Doenças do Cão/virologia , Enterite/veterinária , Infecções por Parvoviridae/veterinária , Salmonelose Animal/microbiologia , Salmonella/isolamento & purificação , Envelhecimento , Animais , Estudos de Casos e Controles , Estudos de Coortes , Coinfecção , Doenças do Cão/microbiologia , Cães , Enterite/complicações , Fezes/microbiologia , Estudos Longitudinais , Infecções por Parvoviridae/complicações , Prevalência , Estudos Prospectivos , Salmonelose Animal/complicações
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