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1.
Arch Virol ; 165(10): 2317-2322, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32643035

RESUMO

Canine kobuviruses (CaKoV) have been found in healthy and diarrheic dogs as well as asymptomatic wild carnivores in various countries. In order to investigate the prevalence and evolution of CaKoV in Tangshan, China, 82 dog fecal samples from pet hospitals in Tangshan were subjected to RT-PCR targeting a segment of the 3D gene of CaKoV. Using this method, we identified CaKoV in 14 samples (17.07%, 14/82). Of the CaKoV-positive samples, 78.57% (11/14) and 50% (7/14) were positive for canine parvovirus and canine coronavirus, respectively. The nucleotide sequences of the 14 strains 96.6%-100% identical to each other and 77.6%-99.2% identical to representative sequences from the NCBI GenBank database. We also amplified the 14 VP1 gene sequences and found that they were 93.3%-99.6% identical to each other and 73.3%-97.8% identical to representative sequences from the NCBI GenBank database. Phylogenetic analysis revealed that the 14 CaKoV strains from Tangshan are closely related to those identified in China and Thailand and display less similarity to those found in Africa, the United States, and Europe. Our data suggest that CaKoV circulated in young pet dogs in Tangshan and displays a high co-infection rate with CCoV and CPV. However, the relationship between the three viruses and their roles in the host requires further investigation.


Assuntos
Doenças do Cão/epidemiologia , Kobuvirus/classificação , Kobuvirus/genética , Infecções por Picornaviridae/veterinária , Animais , China/epidemiologia , Coinfecção/epidemiologia , Coinfecção/veterinária , Coinfecção/virologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/veterinária , Infecções por Coronavirus/virologia , Coronavirus Canino/genética , Doenças do Cão/virologia , Cães/virologia , Feminino , Genes Virais , Masculino , Epidemiologia Molecular , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/veterinária , Infecções por Parvoviridae/virologia , Parvovirus Canino/genética , Animais de Estimação/virologia , Filogenia , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/virologia , Prevalência , Proteínas Estruturais Virais/genética
2.
Arch Virol ; 165(9): 2087-2089, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32524264

RESUMO

A novel picornavirus, referred to as Duck/FC22/China/2017, was isolated from breeding ducks in China and genetically characterized by conducting metagenomics studies. The complete genome consists of a single-stranded, positive-sense RNA made up of 7448 nucleotides (nt) and follows the common picornavirus genome layout: 5' UTR-VP0-VP3-VP1-2A-2B-2C-3A-3B-3C-3D-3' UTR. A typical type-IV internal ribosomal entry site and a conserved 'barbell-like' structure were identified in the 5' UTR and 3' UTR, respectively. The unique 6423-nt open reading frame was predicted to encode a 2141-amino-acid (aa) polyprotein precursor. A pairwise aa sequence identity comparison and phylogenetic analysis revealed that Duck/FC22/China/2017 is closely related to duck aalivirus, duck hepatitis A virus, turkey avisivirus, and red-crowned crane picornavirus.


Assuntos
Genoma Viral , Infecções por Picornaviridae/veterinária , Picornaviridae/isolamento & purificação , Doenças das Aves Domésticas/virologia , Animais , Sequência de Bases , China , Patos/virologia , Filogenia , Picornaviridae/classificação , Picornaviridae/genética , Infecções por Picornaviridae/virologia , Proteínas Virais/genética
3.
Vet Clin North Am Exot Anim Pract ; 23(2): 249-261, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32327034

RESUMO

Recently, multiple infectious organisms have been identified as the cause of emerging diseases in lagomorphs. The most important of these emerging diseases is rabbit hemorrhagic disease virus (RHDV) type 2, a new variant with differences in pathogenicity to classical RHDV. Hepatitis E is considered an emerging zoonotic infectious disease, with widespread prevalence in many different rabbit populations. Mycobacteriosis has been recently reported in other captive domestic rabbit populations. This article provides a recent review of the published literature on emerging infectious diseases in rabbits, including farmed, laboratory, and pet rabbits, some of which have zoonotic potential.


Assuntos
Infecções por Caliciviridae/veterinária , Doenças Transmissíveis Emergentes/veterinária , Hepatite E/veterinária , Infecções por Mycobacterium/veterinária , Infecções por Parvoviridae/veterinária , Infecções por Picornaviridae/veterinária , Animais , Infecções por Caliciviridae/epidemiologia , Doenças Transmissíveis Emergentes/epidemiologia , Vírus da Doença Hemorrágica de Coelhos/isolamento & purificação , Vírus da Doença Hemorrágica de Coelhos/patogenicidade , Hepatite E/epidemiologia , Humanos , Infecções por Mycobacterium/epidemiologia , Infecções por Parvoviridae/epidemiologia , Infecções por Picornaviridae/epidemiologia , Coelhos , Zoonoses
4.
Res Vet Sci ; 130: 207-211, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32200161

RESUMO

Senecavirus A (SVA), also known as Seneca Valley virus (SVV), is an emerging infectious pathogen which have been detected in swine herds from the Brazil, USA, Colombia, Thailand, Canada and some provinces in China, suggesting an increasing geographic distribution of this novel virus. Here, we isolated and characterized a SVV, designated SVA CH-GX-01-2018, thought to be responsible for typical vesicular lesions on the snouts and hooves of finishing pigs from a swine herds in Guangxi province, China, in August 2018. Phylogenetic analysis and sequence alignment indicated that this SVA CH-GX-01-2018 strain was closely related to the strains isolated in 2017 in Guangdong province, a neighboring province of Guangxi, South China, with 98.6% identity at the genome nucleotide level. Our findings characterized a novel SVV infection in pigs from South China and emphasize the importance of surveillance, reinforcing biosecurity measures and developing vaccines to prevent the spread of this viral pathogen.


Assuntos
Infecções por Picornaviridae/veterinária , Picornaviridae/isolamento & purificação , Doenças dos Suínos/virologia , Animais , China , Filogenia , Picornaviridae/classificação , Infecções por Picornaviridae/virologia , RNA Viral/análise , Análise de Sequência de RNA/veterinária , Suínos
5.
Arch Virol ; 165(4): 993-1001, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32037488

RESUMO

Teschovirus A is currently the sole species in the genus Teschovirus, whose members are divided into 13 subtypes: porcine teschovirus (PTV) 1-13. However, recent discoveries of novel PTV genotypes have suggested that a new species, "Teschovirus B", should be established. Here, we have identified six of the 19 known genotypes and two novel genotypes (PTV 17-18), revealing the high genetic diversity of the PTV subpopulation in indigenous pigs of western Jiangxi, China. Moreover, we determined the nearly complete genome sequences of PTV 17-SG9 and PTV 18-SG10. Together with PTV 1-13, these novel genotypes were confirmed to be members of the species Teschovirus A based on phylogenetic and genetic divergence analysis. Consequently, the species Teschovirus A now includes at least 15 PTV genotypes.


Assuntos
Infecções por Picornaviridae/veterinária , Doenças dos Suínos/virologia , Teschovirus/genética , Teschovirus/isolamento & purificação , Animais , Sequência de Bases , China , Genoma Viral , Genótipo , Filogenia , Infecções por Picornaviridae/virologia , Suínos , Teschovirus/classificação
6.
Arch Virol ; 165(2): 355-366, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31845156

RESUMO

Picornaviruses infect a wide range of mammals including livestock such as cattle and swine. As with other picornavirus genera such as Aphthovirus, there is emerging evidence of a significant economic impact of livestock infections caused by members of the genera Enterovirus and Kobuvirus. While the human-infecting enteroviruses and kobuviruses have been intensively studied during the past decades in great detail, research on livestock-infecting viruses has been mostly limited to the genomic characterization of the viral strains identified worldwide. Here, we extend our previous studies of the structure and function of the complexes composed of the non-structural 3A proteins of human-infecting enteroviruses and kobuviruses and the host ACBD3 protein and present a structural and functional characterization of the complexes of the following livestock-infecting picornaviruses: bovine enteroviruses EV-E and EV-F, porcine enterovirus EV-G, and porcine kobuvirus AiV-C. We present a series of crystal structures of these complexes and demonstrate the role of these complexes in facilitation of viral replication.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Infecções por Enterovirus/metabolismo , Enterovirus Bovino/patogenicidade , Enterovirus Suínos/patogenicidade , Kobuvirus/patogenicidade , Proteínas de Membrana/metabolismo , Infecções por Picornaviridae/metabolismo , Animais , Bovinos , Linhagem Celular , Infecções por Enterovirus/veterinária , Infecções por Enterovirus/virologia , Enterovirus Suínos/genética , Células HEK293 , Humanos , Kobuvirus/genética , Infecções por Picornaviridae/veterinária , Infecções por Picornaviridae/virologia , Suínos , Proteínas Virais/genética , Proteínas Virais/metabolismo , Replicação Viral/genética
7.
Res Vet Sci ; 128: 286-292, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31869594

RESUMO

Recent results on the detection and genetic characterization of stool-associated RNA viruses from different species have increased the knowledge about the extreme genetic diversity of picornaviruses. This study aimed to investigate the presence of unclassified porcine stool-associated RNA viruses (posaviruses) in South Korea and to elucidate the molecular evolution of the viruses. By RT-PCR, posaviruses 1 and 3 were exclusively found in fecal samples and consistently detected in three consecutive years in six of eight provinces, with 148/697 (21.2%) and 33/84 (39.3%) positive samples and farms, respectively. Every age group but the older age groups (finisher, sow) had significantly higher positive rates of posavirus 1 than posavirus 3. An analysis of the RNA-dependent RNA polymerase sequences by likelihood mapping and maximum-likelihood-based phylogenetic analysis revealed that stool-associated RNA viruses formed four supergroups that were well separated from all recognized families of the order Picornavirales. Five genomes of Korean posaviruses generated in this study were phylogenetically grouped with posavirus 1 and posavirus 3 and were predicted to have the typical genome organization of picornaviruses.


Assuntos
Infecções por Picornaviridae/veterinária , Picornaviridae , Doenças dos Suínos/virologia , Animais , Fezes/virologia , Genes Virais , Genoma Viral , Filogenia , Picornaviridae/classificação , Picornaviridae/genética , Picornaviridae/isolamento & purificação , RNA Replicase/genética , República da Coreia , Suínos
8.
J Gen Virol ; 101(2): 175-187, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31859611

RESUMO

Senecavirus A (SVA) is an emerging picornavirus that causes vesicular disease (VD) in swine. The virus has been circulating in swine in the United Stated (USA) since at least 1988, however, since 2014 a marked increase in the number of SVA outbreaks has been observed in swine worldwide. The factors that led to the emergence of SVA remain unknown. Evolutionary changes that accumulated in the SVA genome over the years may have contributed to the recent increase in disease incidence. Here we compared full-genome sequences of historical SVA strains (identified before 2010) from the USA and global contemporary SVA strains (identified after 2011). The results from the genetic analysis revealed 6.32 % genetic divergence between historical and contemporary SVA isolates. Selection pressure analysis revealed that the SVA polyprotein is undergoing selection, with four amino acid (aa) residues located in the VP1 (aa 735), 2A (aa 941), 3C (aa 1547) and 3D (aa 1850) coding regions being under positive/diversifying selection. Several aa substitutions were observed in the structural proteins (VP1, VP2 and VP3) of contemporary SVA isolates when compared to historical SVA strains. Some of these aa substitutions led to changes in the surface electrostatic potential of the structural proteins. This work provides important insights into the molecular evolution and epidemiology of SVA.


Assuntos
Doenças Transmissíveis Emergentes , Infecções por Picornaviridae/veterinária , Picornaviridae/genética , Doenças dos Suínos/virologia , Substituição de Aminoácidos/genética , Animais , Doenças Transmissíveis Emergentes/veterinária , Doenças Transmissíveis Emergentes/virologia , Surtos de Doenças , Evolução Molecular , Variação Genética , Genoma Viral , Filogenia , Infecções por Picornaviridae/epidemiologia , Suínos , Doenças dos Suínos/epidemiologia , Estados Unidos/epidemiologia , Proteínas Virais/genética , Proteínas Estruturais Virais/genética
9.
J Vet Diagn Invest ; 31(6): 909-912, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31650901

RESUMO

Swine diarrhea can be caused by multiple agents, including porcine epidemic diarrhea virus (PEDV), porcine sapelovirus (PSV), and porcine sapovirus (SaV). We designed a one-step triplex reverse-transcription PCR (RT-PCR) detection method including 3 pairs of primers that focused on the S1 gene of PEDV, a conserved gene of PSV, and the VP1 gene of SaV. The optimal concentrations of upstream and downstream primers in the triplex RT-PCR were 0.24 µM for PEDV, 0.15 µM for PSV, and 0.2 µM for SaV, and the optimal annealing temperature was 55.5°C. Triplex RT-PCR assessment of 402 piglet diarrhea samples was compared with conventional individual RT-PCR. Concordance rates in both tests for individual viruses were 100%, 97.6%, and 94.4% for PEDV, PSV, and SaV, respectively. PEDV, PSV, and SaV were detected in 57.2%, 10.4%, and 9.0% of the samples, respectively. The high sensitivity and specificity of this triplex RT-PCR-based detection method for PEDV, PSV, and SaV could allow rapid detection and analysis of mixed infections by these 3 viruses.


Assuntos
Infecções por Caliciviridae/veterinária , Infecções por Coronavirus/veterinária , Diarreia/veterinária , Reação em Cadeia da Polimerase Multiplex/veterinária , Infecções por Picornaviridae/veterinária , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Doenças dos Suínos/diagnóstico , Animais , Infecções por Caliciviridae/diagnóstico , Infecções por Coronavirus/diagnóstico , Diarreia/diagnóstico , Reação em Cadeia da Polimerase Multiplex/métodos , Picornaviridae/isolamento & purificação , Infecções por Picornaviridae/diagnóstico , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Sapovirus/isolamento & purificação , Suínos , Doenças dos Suínos/virologia
10.
Can J Vet Res ; 83(4): 242-247, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31571722

RESUMO

The objective of this study was to investigate whether a virulent Canadian isolate of Senecavirus A (SVA) causes idiopathic vesicular disease (IVD) in pigs. Senecavirus A, which was first isolated in the United States in 2002 as Seneca Valley Virus, was linked to cases of porcine idiopathic vesicular disease in Canada in 2007 and in the United States in 2010. Since 2014, SVA outbreaks in Brazil, the US, Canada, China, Thailand, and Colombia point to an expanding global distribution and the need to study the pathogenicity of the virus. Unlike the prototype virus, recent US isolates of SVA have been shown to cause vesicular disease in pigs. We report vesicular disease in pigs following experimental inoculation with a 2016 Canadian isolate of SVA. All inoculated pigs developed vesicular lesions regardless of route of inoculation. Virus was detected in blood and oral fluids as well as on oral and fecal swabs. In addition, all pigs seroconverted to SVA by 6 days post-inoculation (DPI). This study confirms that recent Canadian isolates of SVA cause vesicular disease in pigs and highlights the importance of monitoring SVA for increased virulence.


Assuntos
Infecções por Picornaviridae/veterinária , Picornaviridae/patogenicidade , Doenças dos Suínos/virologia , Animais , Anticorpos Antivirais/sangue , Canadá/epidemiologia , Ensaio de Imunoadsorção Enzimática , Testes de Neutralização , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/patologia , Infecções por Picornaviridae/virologia , Suínos , Doenças dos Suínos/patologia , Virulência
11.
Acta Vet Hung ; 67(3): 463-476, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31549549

RESUMO

The involvement of picornaviruses in calf diarrhoea was evaluated by the analysis of 127 faecal samples collected from diarrhoeic calves during 2014-2016. Virus detections were carried out by PCR using generic or specific primer pairs. One-third of the faecal samples (33.86%) were found to be positive for one or more of the studied viruses. Bovine kobuvirus was detected in 22.83%, bovine hungarovirus in 11.02%, while bovine enterovirus 1 in 5.51% of the samples. The sequences of the PCR products indicated the existence of novel variants in all the three virus species. When comparing the partial sequences, the nucleotide sequence identities between our newly detected viruses and those previously deposited to the GenBank ranged between 76 and 99%. Phylogenetic analyses revealed a novel lineage within the species Hunnivirus A. Our findings suggest that these viruses should be regarded as possible aetiological agents of calf diarrhoea. Based on the newly determined sequences, we designed and tested a new generic PCR primer set for the more reliable detection of bovine hungaroviruses. This is the first report on the molecular detection of the presence of bovine hungarovirus, bovine kobuvirus and bovine enterovirus 1 in the faecal samples of diarrhoeic calves in Turkey.


Assuntos
Doenças dos Bovinos/virologia , Diarreia/veterinária , Infecções por Picornaviridae/veterinária , Picornaviridae/isolamento & purificação , Animais , Bovinos , Diarreia/virologia , Enterovirus Bovino/genética , Enterovirus Bovino/isolamento & purificação , Kobuvirus/genética , Kobuvirus/isolamento & purificação , Picornaviridae/genética , Infecções por Picornaviridae/virologia , Turquia
12.
Microb Pathog ; 136: 103695, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31449854

RESUMO

Seneca Valley virus (SVV) has been identified as the causative agent of SVV-associated vesicular disease (SAVD). To investigate the pathogenicity of two newly isolated SVV strains (GD-S5/2018 and GD04/2017) in China, experimental infections of pigs were performed. In pig experiments, both SVV strains successfully infected all animals, evidenced by presence of virus shedding and robust protective antibody responses. SVV GD-S5/2018 infection resulted in characteristic clinical signs, and ulcerative lesions on the tongue and gums. However, SVV GD04/2017 did not cause any clinical symptoms except depression in pigs during the experiment. Taken together, these results demonstrate that SVV GD-S5/2018 is a virulent strain for pigs, whereas SVV GD04/2017 is nearly avirulent. The established animal models for SVV infection will be utilized to dissect the immunity and pathogenesis, and develop vaccines and antivirals.


Assuntos
Infecções por Picornaviridae/veterinária , Picornaviridae/patogenicidade , Doença Vesicular Suína/patologia , Doença Vesicular Suína/virologia , Animais , China , Picornaviridae/isolamento & purificação , Infecções por Picornaviridae/patologia , Infecções por Picornaviridae/virologia , Suínos , Virulência
13.
Vet Microbiol ; 235: 319-326, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31383319

RESUMO

Porcine kobuviruses are widely distributed in swine, but the clinical significance of these viruses remains unclear, since they have been associated with both diarrheic and healthy pigs. In addition, there is a paucity of data on Kobuvirus prevalence in Canadian pig herds. In this study, a total of 181 diarrheic and healthy piglets were monitored and sampled on four occasions, intended to represent the different stages of production. The piglets were sampled at the nursing farms (birth to weaning stage), at the nursery farms (post-weaning stage), and at finishing farms (at the beginning and the end of the fattening stage). Fecal and environmental samples were collected during each life stage. Following viral extraction, Kobuvirus detection by RT-PCR was conducted, and positive samples were sequenced. During the late-nursing stage (6-21 days old), piglets with diarrhea shed more Kobuvirus than healthy individuals. Piglets shed more Kobuvirus during the post-weaning stage (nursery farms) than during any of the other life stages. This was evidenced in individual samples as well as in environmental samples. Over 97% of the sampled piglets shed Kobuvirus at least once in their lifetime. All piglets shedding a Kobuvirus strain or mix of strains at the nursing stage did not appear to shed another porcine kobuvirus strain at a later life stage. Overall, our findings throw light on Kobuvirus shedding dynamics and their potential role in neonatal diarrhea at the nursing stage, which appears to be the point of entry for kobuviruses into swine production systems.


Assuntos
Diarreia/veterinária , Kobuvirus/fisiologia , Infecções por Picornaviridae/veterinária , Doenças dos Suínos/virologia , Eliminação de Partículas Virais , Fatores Etários , Animais , Diarreia/virologia , Fazendas , Fezes/virologia , Kobuvirus/isolamento & purificação , Filogenia , Infecções por Picornaviridae/virologia , RNA Viral , Análise de Sequência de DNA , Suínos , Desmame
14.
Poult Sci ; 98(12): 6333-6339, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31393586

RESUMO

Duck hepatitis A virus (DHAV) is one of the pathogens that cause fatal duck viral hepatitis (DVH) in ducklings, which is an acute and contagious disease with a high mortality rate. Despite a continuing official duck vaccination program, DHAV infection remains a major threat to the duck industry. Considerable changes were observed in the epidemiology of DHAV-1/-3 in China over time. Therefore, comparing the pathogenicity of different DHAV serotypes can provide a theoretical basis for the diagnosis and prevention of DVH. In this study, we systematically investigated the effects of infection with DHAV-1/-3 field strains on clinical signs, gross lesions, histopathological changes, viral RNA detection, enzymatic systems, and metabolite concentrations. The results demonstrated that the major macroscopic and microscopic lesions in ducks infected with DHAV-1/-3 in the liver, brain, spleen, pancreas, and kidneys exhibited no significant differences. After 24 h of infection, DHAV quickly appeared in blood and major organs. Significant changes in clinical chemical markers together with histopathological lesions and viral RNA detection indicated that the liver is the major target organ for both viruses, resulting in impaired of liver integrity and function. In addition, we found that both viruses were able to invade both central and peripheral immune organs. Also lipase plasma activity was substantially affected by DHAV-1/-3, indicating that the integrity and function of the pancreas was compromised. However, there was no significant difference in pathogenicity between DHAV-1 and -3. The results of this study provide new insights into the pathogenesis of DHAV-1/3, two viruses that cause serious depression, metabolic disorders, and immunosuppression.


Assuntos
Patos , Vírus da Hepatite do Pato/fisiologia , Vírus da Hepatite do Pato/patogenicidade , Hepatite Viral Animal/virologia , Infecções por Picornaviridae/veterinária , Doenças das Aves Domésticas/virologia , Animais , Hepatite Viral Animal/patologia , Infecções por Picornaviridae/patologia , Infecções por Picornaviridae/virologia , RNA Viral/isolamento & purificação , Virulência
15.
PLoS One ; 14(8): e0219750, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31369566

RESUMO

BACKGROUND: The cocirculation of duck hepatitis A virus subtypes 1 (DHAV-1) and 3 (DHAV-3) in ducklings has resulted in significant economic losses. Ducklings with DHAV-1 or DHAV-3 infection show similar clinical signs and gross lesions; hence, it is important to identify the viral subtypes in infected ducklings as early as possible for better clinical management. METHODS AND RESULTS: Based on multiple 5' noncoding region (5'-NCR) sequences of DHAV-1 and DHAV-3 strain alignments, universal and type-specific primers were designed and synthesized. With three primers in one-tube reverse transcription-PCR (RT-PCR), reference DHAV-1 and DHAV-3 isolates ranging over 60 years and across many different countries were successfully amplified, indicating that the primer sequences were completely conserved. The sequence results and the sizes of amplicons from reference DHAV-1 and DHAV-3 isolates are completely correlated with their subtypes. Moreover, with this one-tube RT-PCR system, amplicon sizes from liver samples of reference DHAV-1- or DHAV-3-infected birds fit closely with their subtypes, which was determined by virus isolation and neutralization testing. No other duck-origin RNA viruses were detected. The sensitivity of viral RNA detection was 10 pg. With this system, 20% subtype 1, 45% subtype 3, and 9% coinfection of two subtypes were detected in 55 clinical samples. CONCLUSIONS AND SIGNIFICANCE: This novel approach could be used for rapidly typing DHAV-1 or DHAV-3 infection in routine clinical surveillance or epidemiological screening.


Assuntos
Patos/virologia , Vírus da Hepatite do Pato/classificação , Vírus da Hepatite do Pato/genética , Hepatite Viral Animal/diagnóstico , Infecções por Picornaviridae/veterinária , Doenças das Aves Domésticas/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Animais , Genótipo , Vírus da Hepatite do Pato/isolamento & purificação , Hepatite Viral Animal/virologia , Infecções por Picornaviridae/diagnóstico , Infecções por Picornaviridae/virologia , Doenças das Aves Domésticas/virologia
16.
BMC Vet Res ; 15(1): 254, 2019 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-31324182

RESUMO

BACKGROUND: Canine Kobuvirus (CaKoV) has been detected both in healthy and diarrheic dogs and in asymptomatic wild carnivores. In this study, we conducted a survey of CaKoV at small animal hospitals in Bangkok and vicinity of Thailand during September 2016 to September 2018. RESULTS: Three hundred and seven rectal swab samples were collected from healthy dogs (n = 55) and dogs with gastroenteritis symptoms (n = 252). Of 307 swab samples tested by using one-step RT-PCR specific to 3D gene, we found CaKoV positivity at 17.59% (54/307). CaKoVs could be detected in both sick (19.44%) and healthy (9.09%) animals. In relation to age group, CaKoV could be frequently detected in younger dogs (25.45%). Our result showed no seasonal pattern of CaKoV infection in domestic dogs. In this study, we characterized CaKoVs by whole genome sequencing (n = 4) or 3D and VP1 gene sequencing (n = 8). Genetic and phylogenetic analyses showed that whole genomes of Thai CaKoVs were closely related to Chinese CaKoVs with highest 99.5% amino acid identity suggesting possible origin of CaKoVs in Thailand. CONCLUSIONS: In conclusion, this study was the first to report the detection and genetic characteristics of CaKoVs in domestic dogs in Thailand. CaKoVs could be detected in both sick and healthy dogs. The virus is frequently detected in younger dogs. Thai CaKoVs were genetically closely related and grouped with Chinese CaKoVs. Our result raises the concerns to vet practitioners that diarrhea in dogs due to canine Kobuvirus infection should not be ignored.


Assuntos
Doenças do Cão/virologia , Kobuvirus/genética , Infecções por Picornaviridae/veterinária , Sequência de Aminoácidos , Animais , Doenças do Cão/epidemiologia , Cães , Gastroenterite/veterinária , Genoma Viral , Kobuvirus/isolamento & purificação , Filogenia , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/virologia , Tailândia/epidemiologia
17.
Avian Dis ; 63(1): 68-74, 2019 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31251521

RESUMO

During 2015, duck farms (n = 27) in Sharkia Province, Egypt, experienced several disease outbreaks leading to mortality and nervous manifestations. Upon necropsy, the affected ducklings showed liver lesions, such as hemorrhage or necrosis, suggestive of duck virus hepatitis (DVH). Reverse transcription-PCR (RT-PCR), on the basis of the 3D gene, found duck livers from 21 farms to be positive for duck hepatitis A virus serotype 1 (DHAV-1). All duck breeds (Pekin, Mallard, and Muscovy) were infected. The virus was isolated in embryonated chicken eggs, which showed embryonic mortality (40%-80%) within 5-7 days, stunting or dwarfing (69.6%), and necrotic liver foci (60.9%). The VP1 gene of 11 DHAV-1 strains was characterized by RT-PCR and Sanger sequencing. All study strains were clustered in a monophyletic branch within subclade B2 of Group 4 and were separated from the Egyptian vaccine strain. Several amino acid (aa) residues, such as V129, S142 (only in four strains), L181, G184, and K217, were related to virus attenuation. However, two aa residues (N193 and E205), found in virulent DHAV-1 strains, were also observed in our strains. This study confirms the circulation of DHAV-1 (subclade B2) in Lower Egypt and elucidates the phylogenetic characters of the VP1 genes, which will be useful in following the local trends of DHAV-1 infections. Further studies are indicated to determine the correlation between these mutations and the virulence of the Egyptian DHAV-1 isolates.


Assuntos
Surtos de Doenças/veterinária , Patos , Vírus da Hepatite do Pato/fisiologia , Hepatite Viral Animal/epidemiologia , Infecções por Picornaviridae/veterinária , Doenças das Aves Domésticas/epidemiologia , Animais , Egito/epidemiologia , Hepatite Viral Animal/virologia , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/virologia , Doenças das Aves Domésticas/virologia
19.
J Fish Dis ; 42(9): 1233-1240, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31210360

RESUMO

A mortality event involving an estimated 1,000 adult bluegills (Lepomis macrochirus) was observed in an ice-covered backwater lake of the upper Mississippi River near Alma, Wisconsin, in December of 2017. Macroscopic signs of disease included abdominal distension due to fluid accumulation within the internal organs as well as external and internal haemorrhaging. Histological evaluation revealed chronic peritonitis with peritoneal adhesions in all fish examined. Kidney, spleen and ascites fluid samples were collected from diseased bluegills and examined for the presence of pathogens. Bluegill picornavirus (BGPV) was isolated using tissue cell culture methods utilizing a recently developed, uncharacterized bluegill fry cell line (BF-4), and the presence of this virus was confirmed through molecular identification. The current geographic range, known susceptible hosts as well as historical epizootics associated with BPGV is discussed. The ability of BGPV to cause significant mortality in wild fish further emphasizes the importance of monitoring both wild and hatchery populations for this pathogen.


Assuntos
Doenças dos Peixes/mortalidade , Perciformes , Infecções por Picornaviridae/veterinária , Picornaviridae/isolamento & purificação , Animais , Doenças dos Peixes/virologia , Infecções por Picornaviridae/mortalidade , Infecções por Picornaviridae/virologia , Rios , Wisconsin/epidemiologia
20.
Infect Genet Evol ; 74: 103939, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31247336

RESUMO

In this study, 96 diarrheic and 77 non-diarrheic fecal samples from dairy calves were collected from 14 dairy farms in 4 provinces to investigate the molecular prevalence and genomic characteristics of Bovine Kobuvirus (BKoV) in China. The results showed that the BKoV positive rate for the diarrheic feces (35.42%) was significantly higher than that for the non-diarrheic feces (11.69%, p < 0.001). Interestingly, three potential novel VP1 lineages were identified from 15 complete VP1 sequences, and a unique triple nucleotide insertion which can result in an aa insertion, was first observed in the 11/12 VP0 fragments with 660 bp long in this study, compared with known BKoV VP0 sequences. Moreover, the first Chinese BKoV genome was successfully obtained from a diarrheic fecal sample, named CHZ/CHINA. The open reading frame (ORF) of the genome from strain CHZ/China shares 87.4%-88.3% nucleotide (nt) and 93.7%-96.4% amino acid (aa) identity, compared with the three known genomes of BKoV. Interestingly, phylogenetic tree based on aa sequences of these genomes showed that CHZ/CHINA was clustered into an independent branch, suggesting the strain may represent a novel BKoV strain. The findings contribute to better understanding the molecular characteristics and evolution of BKoV.


Assuntos
Doenças dos Bovinos/virologia , Diarreia/veterinária , Kobuvirus/classificação , Infecções por Picornaviridae/veterinária , Sequenciamento Completo do Genoma/métodos , Animais , Estudos de Casos e Controles , Bovinos , Diarreia/virologia , Fezes/virologia , Tamanho do Genoma , Genoma Viral , Kobuvirus/genética , Kobuvirus/isolamento & purificação , Filogenia , Infecções por Picornaviridae/virologia , RNA Viral/genética
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