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1.
Medicine (Baltimore) ; 99(40): e22223, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33019396

RESUMO

RATIONALE: Mixed gonadal dysgenesis is a rare disorder of sex development, and typically contains a mosaic 45,X/46,XY karyotype. PATIENT CONCERNS: We reported here a case of a 42-year-old man with infertility for 6 years and inability to ejaculate during intercourse. DIAGNOSIS: Physical examination confirmed that the external genitalia was male. The right testis of this patient was resected and the left testis had intrascrotal calcification. Hormone test showed that the level of follicle-stimulating hormone was 20.14 IU/L (normal range, 1.27-19.26 IU/L). No deletion or mutation was found on the sex-determining region Y. H&E staining revealed seminiferous tubule dysgenesis. The karyotyping in peripheral blood and testicular tissue was 45,X/46,XY and 45,X/47,XYY/46,XY, respectively. Based on these results, the patient was diagnosed with 45,X/46,XY or 45,X/47,XYY/46,XY mosaicism and gonadal dysgenesis. INTERVENTIONS: In vitro fertilization and embryo transfer technology were used to help his wife to achieve pregnancy. OUTCOMES: A normal baby boy was born at 36 weeks of gestation with a karyotype 46, XY. LESSONS: We reported a rare case of a karyotype 45,X/46,XY in blood cells and 45,X/47, XYY/46,XY in testicular tissue. In vitro fertilization and embryo transfer technology can help to achieve pregnancy.


Assuntos
Disgenesia Gonadal Mista/genética , Injeções de Esperma Intracitoplásmicas/métodos , Adulto , Feminino , Disgenesia Gonadal Mista/complicações , Disgenesia Gonadal Mista/diagnóstico , Humanos , Infertilidade Masculina/etiologia , Masculino , Mosaicismo , Gravidez , Resultado da Gravidez , Recuperação Espermática
2.
Khirurgiia (Mosk) ; (10): 44-48, 2020.
Artigo em Russo | MEDLINE | ID: mdl-33047585

RESUMO

OBJECTIVE: To reveal the association of tension-free inguinal hernia repair and pathospermia in fertile men. MATERIAL AND METHODS: We have retrospectively analyzed medical records of 512 men who appealed to andrologist with complaints of the absence of pregnancy in wife in 2018. We evaluated duration and features of infertility, presence/absence of previous inguinal hernia repair, spermogram data (according to WHO criteria, 2010) in all patients. RESULTS: Duration of infertility in men after inguinal hernia repair persists for 4.2±2.1 years. Right-sided hernia repair was performed in 36 (48.6%) patients, left-side - 23 (31%), bilateral repair - 15 (20.2%) patients. Men with impaired sperm motility prevailed among patients after right-sided inguinal hernia repair (17 (47.2%) people). Left-sided hernia repair was followed by asthenozoospermia in 8 (34.7%) cases, bilateral hernia repair - in 3 (20%) cases. The most severe abnormalities in semen analysis (azoospemia) develop after bilateral hernia repair. CONCLUSION: Inguinal tension-free hernia repair is a risk factor for male infertility in 14.4% of cases. It is very important to examine a man in case of infertile marriage. Previous surgical interventions including inguinal hernia repair should be considered.


Assuntos
Hérnia Inguinal/cirurgia , Herniorrafia/efeitos adversos , Infertilidade Masculina/etiologia , Astenozoospermia/diagnóstico , Astenozoospermia/etiologia , Azoospermia/diagnóstico , Azoospermia/etiologia , Herniorrafia/métodos , Humanos , Infertilidade Masculina/diagnóstico , Masculino , Estudos Retrospectivos , Fatores de Risco , Análise do Sêmen
3.
Artigo em Inglês | MEDLINE | ID: mdl-33006953

RESUMO

Severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) that causes COVID-19 infections penetrates body cells by binding to angiotensin-converting enzyme-2 (ACE2) receptors. Evidence shows that SARS-CoV-2 can also affect the urogenital tract. Hence, it should be given serious attention when treating COVID-19-infected male patients of reproductive age group. Other viruses like HIV, mumps, papilloma and Epstein-Barr can induce viral orchitis, germ cell apoptosis, inflammation and germ cell destruction with attending infertility and tumors. The blood-testis barrier (BTB) and blood-epididymis barrier (BEB) are essential physical barricades in the male reproductive tract located between the blood vessel and seminiferous tubules in the testes. Despite the significant role of these barriers in male reproductive function, studies have shown that a wide range of viruses can still penetrate the barriers and induce testicular dysfunctions. Therefore, this mini-review highlights the role of ACE2 receptors in promoting SARS-CoV-2-induced blood-testis/epididymal barrier infiltration and testicular dysfunction.


Assuntos
Barreira Hematotesticular/enzimologia , Barreira Hematotesticular/patologia , Infecções por Coronavirus/enzimologia , Infecções por Coronavirus/patologia , Infertilidade Masculina/etiologia , Infertilidade Masculina/patologia , Peptidil Dipeptidase A/metabolismo , Pneumonia Viral/enzimologia , Pneumonia Viral/patologia , Humanos , Infertilidade Masculina/enzimologia , Masculino , Pandemias , Testículo/metabolismo
5.
PLoS One ; 15(9): e0232306, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32986707

RESUMO

Tsetse eradication continues to be a top priority for African governments including that of Senegal, which embarked on a project to eliminate Glossina palpalis gambiensis from the Niayes area, following an area-wide integrated pest management approach with an SIT component. A successful SIT programme requires competitive sterile males of high biological quality. This may be hampered by handling processes including irradiation and the release mechanisms, necessitating continued improvement of these processes, to maintain the quality of flies. A new prototype of an automated chilled adult release system (Bruno Spreader Innovation, (BSI™)) for tsetse flies was tested for its accuracy (in counting) and release rate consistency. Also, its impact on the quality of the released sterile males was evaluated on performance indicators, including flight propensity, mating competitiveness, premating and mating duration, insemination rate of mated females and survival of male flies. The BSITM release system accurately counted and homogenously released flies at the lowest motor speed set (0.6 rpm), at a consistent rate of 60±9.58 males/min. Also, the release process, chilling (6 ± 1°C) and passing of flies through the machine) had no significant negative impact on the male flight propensity, mating competitiveness, premating and mating durations and the insemination rates. Only the survival of flies was negatively affected whether under feeding or starvation. The positive results of this study show that the BSI™ release system is promising for use in future tsetse SIT programmes. However, the negative impact of the release process on survival of flies needs to be addressed in future studies and results of this study confirmed under operational field conditions in West Africa.


Assuntos
Temperatura Baixa/efeitos adversos , Infertilidade Masculina/veterinária , Controle Biológico de Vetores/métodos , Tripanossomíase Africana/prevenção & controle , Moscas Tsé-Tsé/fisiologia , Animais , Vetores de Doenças , Feminino , Voo Animal/fisiologia , Humanos , Infertilidade Masculina/etiologia , Gado/parasitologia , Masculino , Controle Biológico de Vetores/instrumentação , Senegal , Comportamento Sexual Animal/fisiologia , Trypanosoma/patogenicidade , Tripanossomíase Africana/parasitologia , Tripanossomíase Africana/veterinária , Moscas Tsé-Tsé/parasitologia
6.
Medicine (Baltimore) ; 99(28): e20813, 2020 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32664073

RESUMO

INTRODUCTION: As one of the most common causes of male infertility, asthenozoospermia mainly shows low sperm motility, accounting for 81.84% of male infertility patients. Recently, there has been a notable increase for relationship between genetic testing and asthenozoospermia. In this report, we design to provide clues to prove relationship between dynein heavy chain gene 5 (DNAH5) gene alterations and asthenozoospermia. This also provides a reference for patients to choose a reasonable treatment plan or genetic counseling to assist reproductive reproduction. PATIENTS CONCERN: In the present study, we screened 143 patients with asthenozoospermia for variants in DNAH5 gene. We used high-throughput targeted gene sequencing technology and the data were assessed by bioinformatics analysis. DIAGNOSIS: We found 1 of 143 asthenozoospermia patients was detected as carrying DNAH5 compound heterozygous variants (c.3502G>A and c.2578-11_2578-7del). OUTCOMES: The variation c.2578-11_2578-7del was predicted in silico to not affect the splicing by HSF3. The variation c.3502G > A (p.E1168K) may cause disease by Mutationtaster software. They may contribute to a risk of male infertility in Chinese patients. CONCLUSIONS: We discussed the possible association between mutations in DNAH5 and asthenospermia for the first time in Chinese people. If confirmed in larger samples and different races, this result was meaningful for a better diagnosis of asthenospermia patients.


Assuntos
Astenozoospermia/genética , Dineínas/metabolismo , Mutação/genética , Adulto , Grupo com Ancestrais do Continente Asiático/etnologia , Grupo com Ancestrais do Continente Asiático/genética , Dineínas do Axonema/genética , Biologia Computacional/instrumentação , Humanos , Infertilidade Masculina/etiologia , Masculino , Polimorfismo de Nucleotídeo Único/genética , Motilidade Espermática/genética
8.
Am J Physiol Endocrinol Metab ; 319(2): E291-E304, 2020 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-32603600

RESUMO

Several studies have reported that health problems occur in assisted reproductive technology (ART)-conceived offspring. Recently, investigations have demonstrated that paternal environmental conditions influence offspring health. However, it is unclear whether the factors that cause male infertility per se affect offspring health and contribute to health problems in ART-born children. Scrotal heat stress represents a common cause for oligoasthenozoospermia, and in these cases, in vitro fertilization-embryo transfer (IVF-ET) is typically recommended for those individuals trying to conceive. We exposed C57BL/6J male mice to frequent and mild scrotal heat stress (fmSHS) (39°C for 30 min once weekly for 5 consecutive wk). Sperm was subjected to IVF-ET with oocytes of untreated C57BL/6J females to produce offspring mice. Glucose intolerance and insulin resistance was observed in the male offspring mice derived from fmSHS-exposed fathers. Islets, after evaluation, remained unchanged. Genes involved in glucose metabolism, especially, those in insulin signaling pathways, showed dysregulation in the liver of the fmSHS-derived male offspring. Differentially methylated regions were found in the sperm of fmSHS-exposed mice by whole genome bisulfite sequencing. Interestingly, abnormal methylation of some genes with altered expression in offspring was observed in both the sperm of fmSHS fathers and the liver of their male offspring. Our results suggest that the factors that cause male infertility can affect male offspring health by an epigenetic mechanism.


Assuntos
Epigênese Genética/fisiologia , Glucose/metabolismo , Temperatura Alta , Infertilidade Masculina/genética , Exposição Paterna/efeitos adversos , Escroto/fisiopatologia , Animais , Metilação de DNA , Transferência Embrionária , Feminino , Fertilização In Vitro , Intolerância à Glucose/etiologia , Intolerância à Glucose/patologia , Infertilidade Masculina/etiologia , Resistência à Insulina/genética , Ilhotas Pancreáticas/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Membro 1 do Grupo A da Subfamília 4 de Receptores Nucleares/genética , Transdução de Sinais/genética
9.
Med Hypotheses ; 143: 110083, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32679425

RESUMO

The outbreak of CoronaVirus Disease19 (COVID19) in December 2019 posed a serious threat to public safety, and its rapid spread caused a global health emergency. Clinical data show that in addition to respiratory system damage, some male patients with COVID-19 are also accompanied by abnormal renal function and even renal damage. As the main receptor of syndrome coronavirus 2 (SARS-CoV-2), angiotensin converting enzyme 2 (ACE2) is also found to be highly expressed not only in respiratory mucosa and alveolar epithelial cells, but also in renal tubule cells, testicular Leydig cells and seminiferous tubule cells. This suggests that SARS-CoV-2 has the possibility of infecting the male reproductive system, and the recent detection of SARS-CoV-2 in the patient's semen further confirms this theory. In previous studies, it has been found that ACE2 has the ability to regulate autophagy. Not only that, recent studies have also found that SARS-CoV-2 infection can also lead to a reduction in autophagy. All of these associate SARS-CoV-2 with autophagy. Furthermore, autophagy has been shown to have an effect on male reproduction in many studies. Based on these, we propose the hypothesis that SARS-CoV-2 affects male reproductive function by regulating autophagy. This hypothesis may provide a new idea for future treatment of COVID-19 male patients with reproductive function injury, and it can also prompt medical staff and patients to consciously check their reproductive function.


Assuntos
Autofagia/fisiologia , Betacoronavirus , Infecções por Coronavirus/fisiopatologia , Pneumonia Viral/fisiopatologia , Reprodução/fisiologia , Betacoronavirus/patogenicidade , Infecções por Coronavirus/complicações , Infecções por Coronavirus/patologia , Genitália Masculina/patologia , Genitália Masculina/fisiopatologia , Genitália Masculina/virologia , Humanos , Infertilidade Masculina/etiologia , Infertilidade Masculina/patologia , Infertilidade Masculina/fisiopatologia , Masculino , Modelos Biológicos , Pandemias , Peptidil Dipeptidase A/fisiologia , Pneumonia Viral/complicações , Pneumonia Viral/patologia , Espermatozoides/patologia , Espermatozoides/virologia
11.
Lasers Med Sci ; 35(8): 1671-1680, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32483749

RESUMO

Male infertility is a worldwide critical condition that affects about the 7.5% of males in Europe leading to an increment of the couples referring to reproductive medicine units to achieve pregnancy. Moreover, in the recent years, an increased number of patients have required to freeze their gametes in order to preserve their fertility. Photobiomodulation (PBM) therapy is a potential treatment that has been used for different clinical application basically aimed at biostimulating cells and tissues. Here, we report a deep overview of the published studies, focusing on PBM mechanism of action, with the aim of expanding the knowledge in the field of laser light for a rational utilization of irradiation in the clinical practice. In the field of reproductive science, PBM was employed to increment spermatozoa's metabolism, motility, and viability, due to its beneficial action on mitochondria, leading to an activation of the mitochondrial respiratory chain and to the ATP production. This treatment can be particularly useful to avoid the use of chemicals in the spermatozoa culture medium as well as to promote the spermatozoa survival and movement especially after thawing or in largely immotile sperm samples.


Assuntos
Infertilidade Masculina/radioterapia , Terapia com Luz de Baixa Intensidade , DNA/efeitos da radiação , Humanos , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/etiologia , Masculino , Motilidade Espermática/efeitos da radiação , Espermatozoides/efeitos da radiação
13.
PLoS One ; 15(4): e0232357, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32353040

RESUMO

One of the main molecular causes that contributes to varicocele-related male infertility is excess production of reactive oxygen species (ROS). It is believed that hypoxia is an important stimulator of ROS in this condition. Recently, the significant roles of long non-coding RNAs (lncRNAs) in hypoxia response have emerged. Despite the investigation of hypoxia, there is scant information about the role of hypoxia-responding lncRNAs in varicocele-related male infertility. In the present study, we deduced eight hypoxia-responding lncRNAs based on high-throughput RNA sequencing data from two Gene Expression Omnibus (GEO) datasets. We used qRT-PCR to assess the expression levels of some of these lncRNAs in 42 ejaculated spermatozoa samples from 25 infertile men with varicocele and 17 fertile men as controls. We identified significant increases in expression levels of hypoxia-related lncRNAs, MIR210HG and MLLT4-AS1 in ejaculated spermatozoa of infertile men with varicocele. These lncRNAs also showed significant positive correlations with ROS levels and meaningful negative correlations with sperm parameters (count and motility). Besides, in silico studies identified several hypoxia response elements (HREs) within selected lncRNAs promoters. Delineation of hypoxia-related lncRNAs in varicocele-related infertility provides a valuable insight into male infertility.


Assuntos
Infertilidade Masculina/genética , RNA Longo não Codificante/genética , Varicocele/complicações , Adulto , Hipóxia Celular , Humanos , Infertilidade Masculina/etiologia , Infertilidade Masculina/patologia , Masculino , RNA Longo não Codificante/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Elementos de Resposta , Motilidade Espermática , Espermatozoides/metabolismo , Espermatozoides/fisiologia
14.
Metabolism ; 107: 154229, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32289345

RESUMO

Chronic energy deficiency can impair the hypothalamic-pituitary-gonadal (HPG) axis and lead to hypothalamic anovulation in underweight women. This review presents the syndromes related to underweight status that are associated with infertility, summarizes the underlying mechanisms, and reviews the available treatment options. Eating disorders, such as anorexia nervosa (AN), constitute the most common cause of infertility in underweight women, who, in addition, experience miscarriages, and sexual dysfunction. The relative energy deficiency in sports (RED-S; former terminology: athlete's triad) involves menstrual dysfunction due to low energy availability, which results in anovulation. Moreover, lipodystrophies, malnutrition, starvation, systematic illnesses (malignancies, endocrinopathies, infectious diseases, advanced chronic diseases, neurologic illnesses), and the utilization of drugs can cause excessive weight loss. They may result in fertility problems due to the loss of adipose tissue and the subsequent hormonal disturbances. Each of these conditions requires multidisciplinary management. Nutritional counseling should target the restoration of energy balance by increasing intake and reducing output. Medical treatment, recommended only for patients who did not respond to standard treatment, may include antipsychotics, antidepressants, or leptin administration. Finally, psychiatric treatment is considered an integral part of the standard treatment.


Assuntos
Infertilidade Feminina/etiologia , Infertilidade Masculina/etiologia , Reprodução , Magreza/complicações , Anorexia Nervosa , Transtornos da Alimentação e da Ingestão de Alimentos/complicações , Transtornos da Alimentação e da Ingestão de Alimentos/epidemiologia , Transtornos da Alimentação e da Ingestão de Alimentos/fisiopatologia , Feminino , Humanos , Infertilidade Feminina/epidemiologia , Infertilidade Masculina/epidemiologia , Masculino , Magreza/epidemiologia , Magreza/fisiopatologia
15.
Georgian Med News ; (299): 26-29, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32242839

RESUMO

According to the statistics of the Russian Federation, the number of patients with male infertility increased by 60.3% over the past 12 years. Objective - to evaluate the activity of peripheral blood leukocytes, the rate of their production of reactive oxygen species, and the DNA fragmentation index in men with non-obstructive azoospermia. To solve this problem, we examined 65 men with non-obstructive idiopathic azoospermia. A control group consisted of 20 healthy fertile men. All patients underwent examination of ejaculate with measurement of levels of reactive oxygen species (ROS) and DNA fragmentation. To identify additional links in the pathogenesis of infertility, we determined the functional activity of peripheral blood leukocytes by registering luminol-dependent chemiluminescence (LCH) of leukocytes. The functional activity of leukocytes in men with azoospermia was found to be 4.5 times higher than in healthy men (p<0.01). We found that with such leukocyte activity the level of ROS and DNA fragmentation was 6 times higher in patients with infertility compared to the control group.


Assuntos
Azoospermia , Fragmentação do DNA , Infertilidade Masculina/etiologia , Leucócitos/fisiologia , Espermatozoides/metabolismo , Humanos , Masculino , Espécies Reativas de Oxigênio , Federação Russa , Motilidade Espermática/fisiologia
16.
BMC Public Health ; 20(1): 461, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32252717

RESUMO

BACKGROUND: Iodine intake is essential in the production of thyroid hormone but very few foods are rich in it. Iodine deficiency or excess iodine level may both lead to thyroid disorders, which further affects human fertility function. The objective of this study is to investigate the relationship between iodine intake and seminal parameters among fertile men in China. METHODS: A total of 1098 couples were recruited by trained physicians at different family planning service stations in 2015. Semen and iodine samples were obtained from male respondents. A questionnaire survey inquired about demographic information from couples. The main outcome variables of semen quality were semen volume, semen concentration, semen motility, and sperm count, and time to pregnancy. Urinary iodine concentration (UIC) was used to measure iodine levels for male respondents. Ordinary least squared regressions and logistic regressions were performed to estimate the association between iodine intake level and semen quality parameters. RESULTS: Male respondents with deficient or excess iodine levels had a 5% higher semen volume relative to those with optimal iodine intake (p < 0.1). Suboptimal iodine intake was negatively associated with semen concentration and semen counts (p < 0.01). Longer time of pregnancy was observed in iodine deficiency and excess group than those in the optimal group (p < 0.01). CONCLUSION: In general, iodine deficiency and excess were both associated with decreasing semen quality parameters in male respondents.


Assuntos
Ingestão de Alimentos/fisiologia , Fertilidade/efeitos dos fármacos , Iodo/urina , Análise do Sêmen , Adulto , China , Dieta/efeitos adversos , Feminino , Humanos , Infertilidade Masculina/etiologia , Iodo/deficiência , Análise dos Mínimos Quadrados , Modelos Logísticos , Masculino , Estado Nutricional , Gravidez
17.
Urol Clin North Am ; 47(2): 129-138, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32272984

RESUMO

A male factor is a contributor in 50% of cases of infertility. Although assisted reproductive techniques can often bypass the need to improve semen parameters, the evaluation of the infertile man remains critical. Current methods for evaluating the infertile man are discussed, beginning with the basic workup that all suspected infertile men should undergo, followed by subsequent evaluation steps. Although the fundamental components of the evaluation have remained consistent, several new tools are available to assist in identifying the underlying etiology. As our understanding of male fertility expands, the technologies available to diagnose and ultimately treat it continue to evolve.


Assuntos
Infertilidade Masculina/diagnóstico , Infertilidade Masculina/etiologia , Testes Genéticos , Humanos , Infertilidade Masculina/genética , Masculino , Anamnese , Exame Físico , Análise do Sêmen
19.
Urol Clin North Am ; 47(2): 165-174, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32272988

RESUMO

Sperm DNA damage reduces pregnancy rates in couples undergoing in vitro fertilization (IVF). Because it has been shown that testicular sperm have lower DNA damage than ejaculated sperm, it is an attractive idea to consider using testicular sperm for IVF for men with high sperm DNA damage. In fact, there are multiple centers throughout the world now offering sperm retrieval for IVF to manage this condition. However, there is insufficient evidence to conclude that testicular sperm improves pregnancy/live birth rates. Further studies are required before offering sperm retrieval as a standard of care to manage high sperm DNA damage.


Assuntos
Dano ao DNA , Fertilização In Vitro/métodos , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/terapia , Recuperação Espermática , Aborto Espontâneo/etiologia , Humanos , Infertilidade Masculina/etiologia , Masculino , Espermatozoides , Testículo/patologia
20.
Urol Clin North Am ; 47(2): 175-183, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32272989

RESUMO

From a fertility perspective, men with azoospermia represent a challenging patient population. When no mature spermatozoa are obtained during a testicular sperm extraction, patients are often left with limited options, such as adoption or the use of donor sperm. However, it has been reported that round spermatids can be successfully injected into human oocytes and used as an alternative to mature spermatozoa. This technique is known as round spermatid injection (ROSI). Despite the limitations of ROSI and diminished clinical success rates, the use of round spermatids for fertilization may have potential as a treatment modality for men with azoospermia.


Assuntos
Azoospermia/patologia , Infertilidade Masculina/terapia , Injeções de Esperma Intracitoplásmicas , Recuperação Espermática , Espermátides/patologia , Azoospermia/etiologia , Azoospermia/fisiopatologia , Feminino , Fertilização In Vitro , Humanos , Infertilidade Masculina/etiologia , Infertilidade Masculina/patologia , Infertilidade Masculina/fisiopatologia , Masculino , Microdissecção , Gravidez , Resultado da Gravidez , Análise do Sêmen , Espermatogênese/fisiologia
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