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1.
Biomed Pharmacother ; 133: 111085, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33378981

RESUMO

Obesity is a significant global health and socio-economic challenge, and considered an important risk factor for poor health outcomes including male reproductive dysfunction and infertility. As excess adiposity causes testicular dysfunction and infertility, novel therapeutic strategies require investigation. Nigella sativa (Ns) seed oil and metformin have both demonstrated a potential positive effect on obesity, although both remain poorly investigated in male fertility. Therefore, this study aimed to determine the effect of Ns oil and metformin on total body weight (TBW), mitochondrial membrane potential (MMP), serum testosterone and semen parameters in an obese animal model. Wistar rats (n = 54) were divided into six groups: normal chow (NC), high sugar diet (HSD) only, HSD and saline, HSD and metformin (75 mg/Kg/day), HSD and Ns (200 mg/Kg/day) (NS200), HSD and Ns (400 mg/Kg/day) (NS400). Intervention was force fed for the last 8 weeks of the 14 weeks dietary exposures. Results showed that the HSD increased TBW (P = 0.001) and reduced sperm concentration (P = 0.013) and progressive motility (P = 0.009) compared to the NC group. Metformin, NS200 and NS400 improved TBW (P = 0.035, P = 0.006 and P = 0.005, respectively) and testosterone (P < 0.001) compared to the HSD saline group, where metformin and NS400 improved sperm concentration (P < 0.001 and P = 0.049, respectively) and MMP (P < 0.001). There were no changes in sperm motility and viability for all experimental exposures, although NS400 (P = 0.047) negatively affected sperm viability. Metformin and Ns may be novel treatment options in obesity-induced infertility, although a potential negative impact on viability is cautioned for high dose Ns. These results warrant further investigation of Ns and Metformin for the management of obese infertile males.


Assuntos
Fármacos Antiobesidade/farmacologia , Fármacos para a Fertilidade Masculina/farmacologia , Fertilidade/efeitos dos fármacos , Infertilidade Masculina/prevenção & controle , Metformina/farmacologia , Obesidade/tratamento farmacológico , Óleos Vegetais/farmacologia , Espermatozoides/efeitos dos fármacos , Testosterona/sangue , Animais , Biomarcadores/sangue , Modelos Animais de Doenças , Infertilidade Masculina/sangue , Infertilidade Masculina/patologia , Infertilidade Masculina/fisiopatologia , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Obesidade/sangue , Obesidade/patologia , Ratos Wistar , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/metabolismo , Espermatozoides/patologia
2.
Fertil Steril ; 114(2): 233-238, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32650948

RESUMO

OBJECTIVE: To investigate the presence of viral RNA in human semen of patients with severe acute-respiratory syndrome coronavirus 2 (SARS-CoV-2) and to evaluate its presence and relevance in semen parameters. DESIGN: Pilot cohort study. SETTING: University hospital. PATIENT(S): Thirty-four men were distributed as: 1) patients in convalescence (patients with confirmed SARS-CoV-2 infection in pharyngeal swab according to reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); 2) negative control group (no antibodies); and 3) patients with an acute infection (detection of SARS-CoV-2 in pharyngeal swab). INTERVENTION: Semen and a blood sample were collected from each individual. MAIN OUTCOME MEASURE(S): Analysis of semen quality according to the World Health Organization standards. Detection of SARS-CoV-2 by RT-PCR in the native semen sample and after density gradient preparation. Confirmation of immunoglobulin (Ig) A und IgG antibodies in the blood. RESULT(S): Eighteen semen samples from recovered men were obtained 8-54 days after absence of symptoms, 14 from control subjects, and 2 from patients with an active COVID-19 infection. No RNA was detected by means of RT-PCR in the semen, including semen samples from two patients with an acute COVID-19 infection. Subjects with a moderate infection showed an impairment of sperm quality. CONCLUSION(S): A mild COVID-19 infection is not likely to affect testis and epididymis function, whereas semen parameters did seem impaired after a moderate infection. SARS-CoV-2 RNA could not be detected in semen of recovered and acute COVID-19-positive men. This suggests no viral transmission during sexual contact and assisted reproductive techniques, although further data need to be obtained.


Assuntos
Betacoronavirus/metabolismo , Infecções por Coronavirus/sangue , Pneumonia Viral/sangue , RNA Viral/sangue , Sêmen/metabolismo , Sêmen/virologia , Adulto , Estudos de Coortes , Infecções por Coronavirus/diagnóstico , Humanos , Infertilidade Masculina/sangue , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/virologia , Masculino , Pessoa de Meia-Idade , Pandemias , Projetos Piloto , Pneumonia Viral/diagnóstico , Estudos Prospectivos , Adulto Jovem
5.
Fertil Steril ; 113(4): 865-875, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32164925

RESUMO

OBJECTIVE: To assess the association between psychological stress and male factor infertility as well as testicular function (semen quality, serum reproductive hormones) and erectile dysfunction. DESIGN: Cross-sectional study. SETTING: University Hospital-based research center. PATIENTS: Men with impaired semen quality were included from infertile couples, and men with naturally conceived pregnant partners were used as a reference population. INTERVENTIONS: Participants completed a questionnaire on health and lifestyle, including a 14-item questionnaire about self-rated psychological stress symptoms and stressful life event (SLEs), had a physical examination performed, delivered a semen sample and had a blood sample drawn. MAIN OUTCOMES: Differences in stress scores (calculated from self-reported stress symptoms) and SLEs between infertile and fertile men were assessed in crude and fully adjusted linear regression models. Secondary outcomes were semen quality, serum reproductive hormones, and erectile dysfunction. RESULTS: Of 423 men, 176 (41.6%) experienced at least one SLE in the 3 months prior to inclusion (50.4%/36.9%: infertile/fertile men, P = .03); ß-coefficient and 95% confidence interval for the difference between the groups on the transformed scale in fully adjusted linear regression models was 0.18 (0.06, 0.30). However, there were no differences in psychological stress symptoms between the two groups (ß-coefficient and 95% confidence interval) on the transformed scale (0.14; -0.02, 0.30). No association between stress (self-reported stress symptoms and SLEs) and testicular function or with erectile dysfunction was found in any of the men. CONCLUSION: Infertile men reported a higher number of SLEs than fertile men but did not report more psychological stress symptoms. Distress and SLEs were not associated with reduced male reproductive function.


Assuntos
Infertilidade Masculina/diagnóstico , Infertilidade Masculina/psicologia , Análise do Sêmen/métodos , Estresse Psicológico/diagnóstico , Estresse Psicológico/psicologia , Testículo/fisiologia , Adulto , Estudos Transversais , Humanos , Infertilidade Masculina/sangue , Acontecimentos que Mudam a Vida , Masculino , Pessoa de Meia-Idade , Estresse Psicológico/sangue , Inquéritos e Questionários , Adulto Jovem
6.
Nutr Health ; 26(1): 53-64, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31992124

RESUMO

BACKGROUND: Studies have shown that the amount of protein in the diet affects the hypothalamic-pituitary-testis axis and sub-optimal quantity reduces male fertility potential in both animals and humans. However, individual research reports on the factors associated with male infertility are collectively uncharacterized. AIM: We systematically reviewed, and meta-analysed animal (rats) studies on the effect of low protein diet on factors associated with male infertility. METHODS: PubMed Central, EMBASE and Scopus databases were searched from inception to 30 March 2019 for the study concepts and related keywords in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol. Data on the outcome measures were extracted and pooled across trials using random-effects model and expressed as mean differences (MD) at a 95% confidence interval (CI). RESULTS: Twelve trials identified from 3327 studies, met our inclusion criteria in the comparison of a low protein diet (2-10% protein) vs control protein diet (17-23% protein). The results showed that a low protein diet caused a significant reduction in the body weight (P = 0.0001) testis weight (P = 0.0001), seminal vesicle weight (P = 0.0003), epididymis weight P = 0.02), serum testosterone (P = 0.001) and follicle-stimulating hormone (FSH) concentrations (P = 0.04) compared with the control treatments. No effect on luteinizing hormone (LH) plasma concentration (P = 0.13) was observed. CONCLUSION: This study revealed that low protein diet caused significant reductions in body weight, testis, epididymis and seminal vesicle weights, serum testosterone and FSH concentration in rats. We infer that sub-optimal protein consumption reduces the gonadal and endocrine function, and consequently male infertility.


Assuntos
Dieta com Restrição de Proteínas/métodos , Infertilidade Masculina/epidemiologia , Animais , Ensaios Clínicos Veterinários como Assunto , Proteínas na Dieta/administração & dosagem , Modelos Animais de Doenças , Hormônio Foliculoestimulante/sangue , Humanos , Infertilidade Masculina/sangue , Hormônio Luteinizante/sangue , Masculino , Ratos , Testículo/metabolismo , Testosterona/sangue
7.
Andrologia ; 52(1): e13433, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31773771

RESUMO

Infertility is somewhat more prevalent in men who are obese. They are also reported to have low sperm concentration, higher fraction of spermatozoa that look morphologically abnormal, higher DNA fragmentation index and evidence of oxidative stress. The precise cause for this remains uncertain. Leptin levels in serum and percentage body fat correlate positively, and obese men therefore usually have elevated serum leptin levels. Although leptin is important for normal reproductive function, but when present in excess, leptin could seriously affect reproductive function in men. Reports on the findings of sperm parameters in obese men, particularly those who are subfertile or infertile, seem to be similar to those reported from studies on normal-weight rats treated with leptin. Collectively, the observations reported in human and experimental animal studies point to leptin as a possible link between infertility and obesity. Herein, we review some findings on sperm function in obese subfertile or infertile men and those from animal studies following leptin treatment, and discuss the possible link between leptin and reproductive dysfunction in obese men. The large amounts of leptin secreted by the adipose tissue and its higher circulating levels could indeed be responsible for the higher prevalence of infertility in obese men.


Assuntos
Infertilidade Masculina/etiologia , Leptina/metabolismo , Obesidade/complicações , Animais , Modelos Animais de Doenças , Humanos , Infertilidade Masculina/sangue , Infertilidade Masculina/epidemiologia , Infertilidade Masculina/metabolismo , Leptina/sangue , Masculino , Obesidade/sangue , Obesidade/metabolismo , Prevalência , Ratos , Contagem de Espermatozoides , Motilidade Espermática
8.
J Urol ; 203(4): 832-840, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31642739

RESUMO

PURPOSE: One of the concerns surrounding cryptorchidism is the risk of impaired fertility. Current guidelines recommend orchiopexy at age 6 to 12 months to optimize fertility outcome. We evaluated the fertility potential of boys with nonsyndromic cryptorchidism who underwent orchiopexy within the recommended age range to clarify the need for eventual supplemental treatment modalities. MATERIALS AND METHODS: We retrospectively evaluated mini-puberty hormones (follicle-stimulating hormone, luteinizing hormone and inhibin B) and testicular biopsies from boys with cryptorchidism who underwent orchiopexy within the first year of life between 2010 and 2019. We histologically analyzed germ cell number and type A dark spermatogonia number per seminiferous tubule cross-section in relation to normal values. RESULTS: Of the 333 boys with nonsyndromic cryptorchidism 83 (25%, 21% with bilateral cryptorchidism) had a reduced number of germ cells. A total of 70 boys (21%) had low serum inhibin B, of whom 32 (46%) had a decreased number of germ cells and 23 (33%) had a decreased number of type A dark spermatogonia (p <0.01). Overall, 75 boys (23%) had no type A dark spermatogonia present. CONCLUSIONS: Despite early and successful orchiopexy, 20% to 25% of boys with cryptorchidism may be at risk for infertility based on hormonal and histological data. Blood test and testicular biopsy are mandatory to identify boys at high risk for infertility, in whom additional treatment modalities and followup may be needed.


Assuntos
Criptorquidismo/cirurgia , Fertilidade/fisiologia , Infertilidade Masculina/epidemiologia , Orquidopexia , Espermatogônias/patologia , Biópsia , Pré-Escolar , Criptorquidismo/complicações , Criptorquidismo/fisiopatologia , Humanos , Lactente , Infertilidade Masculina/sangue , Infertilidade Masculina/etiologia , Infertilidade Masculina/patologia , Inibinas/sangue , Masculino , Estudos Retrospectivos , Medição de Risco , Túbulos Seminíferos/citologia , Túbulos Seminíferos/patologia , Maturidade Sexual/fisiologia , Espermatogônias/citologia , Resultado do Tratamento
9.
Endocr Regul ; 53(4): 231-236, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31734656

RESUMO

OBJECTIVE: There is no reliable treatment for men with idiopathic infertility, but the relationship between severe sperm production and the ratio of estrogen to testosterone levels has been shown. Aromatase is an enzyme that plays an important role in converting testosterone to estradiol and androstenedione to estrogen. Aromatase inhibitors can increase testosterone and androgen production without increasing the amount of estrogen in circulation. The aim of this study was to evaluate the effect of aromatase inhibitor letrozole on the male infertility. METHOD: This pre- and post-quasi-experimental clinical trial was carried out on 41 men with an infertility diagnosis. The basic hormonal profile included FSH, LH, testosterone, and estradiol. The ratio of testosterone to serum estradiol was also calculated and recorded. The sperm analysis was performed before the treatment and the seminal parameters were evaluated and recorded. Patients were then treated with letrozole 2.5 mg daily for 4 months. At the end of 4th months, the hormonal profile was studied and seminal analysis performed and recorded. RESULTS: The levels of FSH, LH, testosterone, and estradiol, and the ratio of testosterone to estradiol increased significantly after letrozole treatment. The sperm concentration, sperm motility, and sperm forward motion significantly increased after letrozole treatment. Sperm morphology only lightly altered. CONCLUSION: The ratio of testosterone to estradiol levels in infertile men treated with aromatase inhibitor improved and caused changes in sperm parameters. Letrozole may be used to improve sperm parameters in infertile men with low serum testosterone to estradiol ratio.


Assuntos
Estradiol/sangue , Infertilidade Masculina/tratamento farmacológico , Letrozol/farmacologia , Sêmen/efeitos dos fármacos , Testosterona/sangue , Adulto , Inibidores da Aromatase/farmacologia , Inibidores da Aromatase/uso terapêutico , Humanos , Infertilidade Masculina/sangue , Infertilidade Masculina/fisiopatologia , Irã (Geográfico) , Letrozol/uso terapêutico , Masculino , Pessoa de Meia-Idade , Análise do Sêmen , Motilidade Espermática/efeitos dos fármacos , Resultado do Tratamento
10.
Andrologia ; 51(10): e13423, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31571259

RESUMO

The aim of our study was to evaluate the relationship between maternal tobacco consumption during pregnancy and sperm parameters and sexual hormonal levels of their sons in adulthood. We conducted a cross-sectional study in four medical institutions in Argentina, between June 1999 and June 2015, among male partners of couples consulting for infertility. At inclusion, a structured interview was conducted to obtain information on the basic demographic, medical, surgical and reproductive history, personal tobacco consumption and that of their parents during pregnancy. Two semen analyses at an interval of 2-4 weeks and a blood hormone evaluation (FSH, LH, prolactin, total testosterone and oestradiol) were then ordered. Analyses using multivariate models adjusted for potential confounders were performed for 537 men. Maternal tobacco consumption during pregnancy was associated with a significantly higher risk of reduced sperm count and elevated total testosterone levels. We did not find any significant association between maternal smoking and other sperm parameters nor other hormone levels. Our study adds evidence concerning the association between maternal tobacco consumption during pregnancy and reduced sperm counts of their sons in adulthood. The results showing an association between elevated total testosterone levels and maternal tobacco consumption need to be replicated.


Assuntos
Infertilidade Masculina/diagnóstico , Exposição Materna/efeitos adversos , Efeitos Tardios da Exposição Pré-Natal/diagnóstico , Análise do Sêmen/estatística & dados numéricos , Fumar/efeitos adversos , Adulto , Estudos Transversais , Estradiol/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Infertilidade Masculina/sangue , Infertilidade Masculina/etiologia , Hormônio Luteinizante/sangue , Masculino , Exposição Materna/estatística & dados numéricos , Gravidez , Efeitos Tardios da Exposição Pré-Natal/sangue , Efeitos Tardios da Exposição Pré-Natal/etiologia , Prolactina/sangue , Fumar/epidemiologia , Testosterona/sangue
11.
Int J Surg ; 71: 101-109, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31561004

RESUMO

PURPOSE: A number of studies have examined the association between vitamin D, fertility and semen quality, however, findings have been inconclusive. Herein, we systematically reviewed available observational studies to elucidate the overall relationship between vitamin D, fertility and semen quality in adult population. METHODS: PubMed, Cochrane's Library, Science Direct, Scopus, Google Scholar and ISI Web of Science databases were searched until December 2018 for all available studies evaluating the association between vitamin D, fertility and semen quality. The Newcastle-Ottawa Quality Assessment Scale was used to examine the quality of each study. RESULTS: A total of 18 studies out of 1843 met our inclusion criteria and were included in our systematic review and meta-analysis. Serum 25(OH)D3 was significantly higher in fertile subjects compared to infertile ones (WMD -0.63; 95% CI, -1.06 to -0.21; P = 0.003). Furthermore, there was a significant association between serum 25(OH)D, sperm motility (WMD -5.84; 95% CI, -10.29 to -1.39; P = 0.01) and sperm progressive motility (WMD -5.24; 95% CI, -8.71 to -1.76; P = 0.003). CONCLUSION: Our findings add to the existing literature supporting the concept that nutrition, especially vitamin D, plays an important role in men's sexual health. It should be noted that because of significant heterogeneity among the included studies, caution is warranted when interpreting the results. Further well-designed prospective cohort studies and clinical trials are needed for better understanding of the relationship between vitamin D and fertility and its components.


Assuntos
Fertilidade/fisiologia , Infertilidade Masculina/sangue , Infertilidade/sangue , Sêmen/fisiologia , Vitamina D/sangue , Adulto , Humanos , Masculino , Estado Nutricional , Estudos Observacionais como Assunto , Estudos Prospectivos , Projetos de Pesquisa , Análise do Sêmen
12.
Andrologia ; 51(10): e13409, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31502328

RESUMO

Human infertility is a worldwide health issue and is the inability to conceive following twelve months of unprotected sexual intercourse. Consistent studies reiterated tobacco abuse to be an important risk factor which adversely effects male fertility. This study aims to determine the correlation of kisspeptin and total testosterone levels in smokeless tobacco, smoking tobacco users and healthy controls. A total of 180 subjects were selected using random sampling technique. Non-fasting blood samples (5 ml) were drawn, and ELISA technique was used for the evaluation of plasma levels of kisspeptin and total testosterone. Total testosterone was found to be significantly high in smokers and smokeless tobacco users, while the level of kisspeptin was found to be significantly high in smokeless tobacco users only as compared to control group. Furthermore, the level of cholesterol was found to be significantly low, whereas HDL and triglycerides were found to be significantly high in smokeless tobacco users relative to control subjects. Findings of this study suggest that tobacco use has impact on HPG axis by affecting kisspeptin level. The increase in kisspeptin level can affect hypothalamic function leading to pituitary and gonadal dysfunction along with impairment of reproduction. The finding that smokeless tobacco significantly raises kisspeptin strengthens the idea that smokeless tobacco use has more potent effects centrally compared to smoking.


Assuntos
Infertilidade Masculina/sangue , Kisspeptinas/sangue , Testosterona/sangue , Fumar Tabaco/efeitos adversos , Tabaco sem Fumaça/efeitos adversos , Adulto , Estudos Transversais , Voluntários Saudáveis , Humanos , Infertilidade Masculina/etiologia , Masculino , Fatores de Risco
13.
Anim Reprod Sci ; 208: 106115, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405477

RESUMO

In this study, there was investigation of the effect of heavy metals on the fertility of dromedary camels. Fourteen camels at the Camel Research Center, King Faisal University, and 41 infertile dromedaries admitted to the Veterinary Teaching Hospital were used for semen evaluation during the breeding season. Seminal plasma and blood serum were collected from all males until analysis. Concentrations of three heavy metals [arsenic (As), cadmium (Cd), and lead (Pb)] were determined in the seminal plasma and serum using an atomic absorption spectrophotometer. The results indicate there are differences (P < 0.05 - P < 0.01) in pH, sperm motility, sperm concentration, and sperm abnormalities between the fertile and infertile male camels. In seminal plasma, there were marked differences (P < 0.01- P < 0.0001) between the control and infertile male camels in As, Cd, and Pb concentrations. In serum, there were differences (P < 0.01 - P < 0.001) between the fertile and infertile camels in serum As, Cd, and Pb concentrations. There was a positive correlation (P < 0.05; r = 0.77 and r = 0.94, respectively) between serum and seminal plasma concentrations of both As and Cd in the infertile dromedaries. In the control group, there was a positive correlation (P < 0.05; r = 0.70) between seminal plasma concentrations of Cd and percent sperm abnormalities. In conclusion, relatively greater seminal plasma and serum concentrations of As, Cd, and Pb are associated with lesser values for semen quality variables and infertility in dromedary camels.


Assuntos
Arsênico/sangue , Cádmio/sangue , Camelus/sangue , Infertilidade Masculina/veterinária , Chumbo/toxicidade , Testículo , Animais , Poluentes Ambientais/sangue , Infertilidade Masculina/sangue , Masculino , Análise do Sêmen
15.
Andrologia ; 51(9): e13367, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31286559

RESUMO

Protamines are considered the most important structure in the sperm nucleus, and they are proteins with a significantly large amount of amino acids carrying a positive charge, which allows the formation of the tight package of the genomic DNA in the spermatozoa. Many authors studied the abnormalities in the protamine 1 (PRM1) and/or protamine 2 (PRM2) genes and reported their possible association with male infertility. The chromosome 16 (16p13.2) carries these genes containing multiple undiscovered single nucleotide polymorphisms. The aim of the present study was to investigate the association of c.-190 C>A transversions that occur in PRM1 with idiopathic infertility in a sample of Egyptian men. It was a case-control study, and blood samples were collected from sixty male patients complaining of idiopathic infertility and forty healthy fertile males. The c.-190 C>A transversion in promotor region protamine 1 gene (rs2301365) was assessed by 5' nuclease assay, using Rotor-Gene Q real-time PCR system. The results of the present study revealed that CA and AA genotypes in PRM1 gene were associated significantly with low sperm concentration and decreased sperm motility (p = 0.001). Cases carrying A allele had a 6.05-fold increased risk for idiopathic infertility than cases carrying the C allele (OR: 6.05, 95% CI: 2.038-17.98 p statistically significant ≤0.05). Analysis of the results revealed that the c.-190 C>A transversion may be involved in the development of male infertility.


Assuntos
Predisposição Genética para Doença , Infertilidade Masculina/genética , Regiões Promotoras Genéticas/genética , Protaminas/genética , Adulto , Alelos , Estudos de Casos e Controles , Egito/epidemiologia , Voluntários Saudáveis , Humanos , Infertilidade Masculina/sangue , Infertilidade Masculina/epidemiologia , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Medição de Risco , Fatores de Risco , Contagem de Espermatozoides , Motilidade Espermática/genética , Adulto Jovem
16.
PLoS One ; 14(7): e0219179, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31276533

RESUMO

Semen abnormality (SA) male infertility has become a worldwide reproductive health problem. The invasive tests (e.g., testicular biopsy) and labor-intensive methods of semen collection severely inhibit diagnosis of male infertility. In addition, the pathogenesis and biological interpretation of male infertility are still obscure. In this report, a total of 84 semen abnormality (SA) patients, diagnosed as teratozoospermia (TE, n = 21), asthenozoospermia (AS, n = 23), oligozoospermia (OL, n = 20), azoospermia (AZ, n = 20), and age-matched healthy controls (HC, n = 29) were analyzed by GC-MS for discrimination analysis and discovery of potential biomarkers. Twenty-three biomarkers were obtained by multivariate statistical method (partial least squares-discriminant analysis, PLS-DA) and univariate statistical method (analysis of variance, ANOVA) with comparisons of TE versus HC, AS versus HC, OL versus HC and AZ versus HC. Based on those biomarkers, the most relevant pathways were mainly associated with the metabolism of carbohydrates, amino acids, and lipids. The principal metabolic alternations in SA male infertility included increased levels of energy-related metabolisms, such as tricarboxylic acid cycle, pyruvate metabolism, glyoxylate and dicarboxylate metabolism, glycine, serine, threonine metabolism and saturated fatty acid metabolism. Furthermore, increased levels of glutathione metabolism were related to oxidative stress. Finally, decreased levels of arginine and proline metabolism and inositol phosphate metabolism were observed. In conclusion, blood plasma metabolomics is powerful for characterizing metabolic disturbances in SA male infertility. From metabolic pathway analysis, energy production, oxidation stress and the released enzyme during spermatogenesis take the primary responsibilities for SA male infertility.


Assuntos
Infertilidade Masculina/metabolismo , Plasma/metabolismo , Sêmen/metabolismo , Adulto , Astenozoospermia/metabolismo , Biomarcadores/metabolismo , China , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Infertilidade Masculina/sangue , Metabolismo dos Lipídeos/fisiologia , Masculino , Redes e Vias Metabólicas/fisiologia , Metabolômica/métodos , Oligospermia/metabolismo , Estresse Oxidativo/fisiologia , Análise do Sêmen/métodos
17.
Andrologia ; 51(9): e13370, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31332817

RESUMO

Kisspeptin, a peptide hormone, plays a pivotal role in fertility and neuroendocrine regulation of hypothalamo-pituitary-gonadal axis. Increased kisspeptin and reproductive hormones are responsible for fertility in male and females. This study aimed to explore the role of kisspeptin on hypothalamo-pituitary-gonadal axis by comparing the levels of kisspeptin in fertile and infertile subjects and identifying single nucleotide polymorphisms (SNPs) of KISS1 gene in exon 2 and exon 3 of infertile male and female cohorts. A cross-sectional study was carried out on 80 males (44 infertile and 36 fertile) and 88 females (44 in each group). Significantly high levels of kisspeptin (KP), follicle-stimulating hormone (FSH), luteinizing hormone and testosterone were observed in fertile male and female subjects except low FSH levels in comparison with infertile female subjects. One polymorphism in exon 2 (E1225K [G/A 3673]) and three in exon 3 (P1945A [C/G 5833]; Insertion of T at 6075; G2026G [C/G 6078]) in infertile group were detected, with low KP and hormonal levels. Male subjects had abnormal sperm parameters and unsuccessful attempt of intracytoplasmic sperm injection in females. Expression of SNP in exon 2 and exon 3 of KISS1 could be responsible for alteration in release of reproductive hormones and gonadal functions, hence causing infertility.


Assuntos
Sistema Hipotálamo-Hipofisário/fisiopatologia , Infertilidade Feminina/genética , Infertilidade Masculina/genética , Kisspeptinas/genética , Adulto , Estudos Transversais , Éxons/genética , Feminino , Hormônio Foliculoestimulante/sangue , Humanos , Infertilidade Feminina/sangue , Infertilidade Feminina/epidemiologia , Infertilidade Masculina/sangue , Infertilidade Masculina/epidemiologia , Kisspeptinas/sangue , Hormônio Luteinizante/sangue , Masculino , Pessoa de Meia-Idade , Mutação , Paquistão/epidemiologia , Polimorfismo de Nucleotídeo Único , Testosterona/sangue
18.
Andrologia ; 51(9): e13343, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31332821

RESUMO

The aim of this systematic review and meta-analysis was to evaluate the effect of vitamin D supplementation on total testosterone (TT) and sex hormone-binding globulin (SHBG) in men. We searched PubMed, Scopus and Web of Science for randomized, controlled trials of vitamin D supplementation in men ≥18 years old up to September 2018, without language restrictions. Meta-analysis was based on a random effects model. The systematic review was registered as CRD42018094498. We identified 3,402 articles, of which eight studies with 10 effect sizes met the inclusion criteria. Vitamin D daily dose equivalents ranged from 600 to 4,000 per day to 60,000 IU per week; duration was 6 weeks to 36 months. In general, vitamin D supplementation had no significant effect on TT (MD = 0.20, 95% CI: -0.20, 0.60, p = 0.336) and SHBG (MD = 1.56, 95% CI: -0.85, 3.97, p = 0.204). Subgroup analysis conducted with duration of prescription, type (daily or weekly), dosing frequency and baseline vitamin D and TT concentration showed that vitamin D did not significantly affect TT. The present study did not find any evidence to support beneficial effect of vitamin D supplementation on TT and SHBG in men. Thus, further large-scale randomised controlled trials are required to evaluate the effects of vitamin D supplementation on androgen in men.


Assuntos
Infertilidade Masculina/prevenção & controle , Globulina de Ligação a Hormônio Sexual/análise , Testosterona/sangue , Deficiência de Vitamina D/tratamento farmacológico , Vitamina D/administração & dosagem , Ensaios Clínicos Controlados como Assunto , Suplementos Nutricionais , Relação Dose-Resposta a Droga , Esquema de Medicação , Humanos , Infertilidade Masculina/sangue , Infertilidade Masculina/etiologia , Masculino , Resultado do Tratamento , Deficiência de Vitamina D/sangue , Deficiência de Vitamina D/complicações
19.
Andrology ; 7(5): 654-661, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31161683

RESUMO

BACKGROUND: Lipid metabolic disorders (dyslipidemia) are constantly increasing in occidental societies and lead to the development of pathologies such as obesity, diabetes, and metabolic syndrome. It has been demonstrated that dyslipidemia can alter the reproductive function. Animal models have recently been used to show that the offspring of dyslipidemic males could also develop such pathologies and that the transgenerational transmission involved post-testicular sperm maturation. These data targeted the essential role of male gamete epididymal maturation and its importance for the health of the offspring. OBJECTIVES: This publication summarizes in the first place experimental data obtained using a mouse model of dyslipidemia-induced post-testicular infertility, knockout mice for the two isoforms of the 'Liver X Receptors' (Lxrα;ß-/- ), the major regulators of cholesterol homeostasis. The impact of a high cholesterol diet (HCD) on the protein YWHAZ (14-3-3 ζ or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein Zeta) was also investigated in our model. MATERIALS AND METHODS: In our mouse model, when young fertile Lxrα;ß-/- males aged three months were fed four weeks with a HCD, they developed an epididymal phenotype leading to infertility. The level of sperm YWHAZ was evaluated by Western blot and its tyrosine phosphorylation state by immunoprecipitation followed by Western blot. RESULTS: Our data revealed that sperm lipid composition and structure were altered, leading to defects of the capacitation-associated signaling pathway. They also showed that both the level and the tyrosine phosphorylation state of YWHAZ were affected by the HCD in sperm cells from Lxrα;ß-/- males. DISCUSSION AND CONCLUSION: YWHAZ could be a new important regulator of capacitation-associated tyrosine phosphorylation and a marker of dyslipidemia-induced infertility.


Assuntos
Proteínas 14-3-3/metabolismo , Colesterol na Dieta/efeitos adversos , Colesterol/sangue , Dislipidemias/patologia , Capacitação Espermática/fisiologia , Maturação do Esperma/fisiologia , Animais , Colesterol/metabolismo , Epididimo/metabolismo , Infertilidade Masculina/sangue , Infertilidade Masculina/patologia , Receptores X do Fígado/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Transdução de Sinais/fisiologia , Espermatozoides/metabolismo
20.
Nutrients ; 11(7)2019 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-31252555

RESUMO

Obesity plays an important role in human fertility in both genders. The same is true for vitamin D, for which accumulating evidence from observational human studies suggests a key role for both male and female fertility. In the latter case, however, robust data from relevant interventional studies are currently lacking. It is also not clear whether obesity and vitamin D deficiency, besides their independent effect on human infertility, act in synergy. Several pathogenetic mechanisms may be proposed as a linkage between vitamin D deficiency and obesity, with respect to infertility. In any case, the independent contribution of vitamin D deficiency in obese infertile states needs to be proven in interventional studies focusing on either vitamin D supplementation in obese or weight loss strategies in vitamin D-deficient infertile patients.


Assuntos
Fertilidade , Infertilidade Feminina/epidemiologia , Infertilidade Masculina/epidemiologia , Obesidade/epidemiologia , Deficiência de Vitamina D/epidemiologia , Vitamina D/sangue , Animais , Biomarcadores/sangue , Suplementos Nutricionais , Feminino , Humanos , Infertilidade Feminina/sangue , Infertilidade Feminina/fisiopatologia , Infertilidade Feminina/terapia , Infertilidade Masculina/sangue , Infertilidade Masculina/fisiopatologia , Infertilidade Masculina/terapia , Masculino , Obesidade/sangue , Obesidade/fisiopatologia , Obesidade/terapia , Gravidez , Prognóstico , Técnicas de Reprodução Assistida , Medição de Risco , Fatores de Risco , Vitamina D/uso terapêutico , Deficiência de Vitamina D/sangue , Deficiência de Vitamina D/tratamento farmacológico , Deficiência de Vitamina D/fisiopatologia
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