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1.
Artigo em Inglês | MEDLINE | ID: mdl-34204255

RESUMO

The purpose of this research is to determine whether users' social interaction tie and trust have a mediating effect on the willingness to use the online healthcare community (OHC) platform on an ongoing basis to respond to food safety crises and monitor food safety practices. During the three-month survey, we conducted an online investigation of users who had experience sharing on the OHC platform and were concerned about food safety. Thereby, three hundred and fifty-two valid questionnaires were received and partial least squares was adopted in this study to test the proposed hypotheses. The empirical results show that perceived critical mass, image, and para-social interaction strengthen the social interaction tie between users and the food safety platform. In addition, this study found that social interaction tie and trust of OHC platform users increased users' willingness to continue using the OHC platform. This research provides OHC platform managers with an in-depth understanding of online social interactions on food safety pages. Moreover, the results of this study can help food business owners, government regulators, hospitals, and physicians to improve the way they use the Web for opinion-led food safety crises and provide insight into the intent of promoting the ongoing use of OHC platforms.


Assuntos
Inocuidade dos Alimentos , Intenção , Atenção à Saúde , Aplicação da Lei , Inquéritos e Questionários
2.
Biosensors (Basel) ; 11(6)2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-34201167

RESUMO

Food detection technologies play a vital role in ensuring food safety in the supply chains. Conventional food detection methods for biological, chemical, and physical contaminants are labor-intensive, expensive, time-consuming, and often alter the food samples. These limitations drive the need of the food industry for developing more practical food detection tools that can detect contaminants of all three classes. Raman spectroscopy can offer widespread food safety assessment in a non-destructive, ease-to-operate, sensitive, and rapid manner. Recent advances of Raman spectroscopic methods further improve the detection capabilities of food contaminants, which largely boosts its applications in food safety. In this review, we introduce the basic principles of Raman spectroscopy, surface-enhanced Raman spectroscopy (SERS), and micro-Raman spectroscopy and imaging; summarize the recent progress to detect biological, chemical, and physical hazards in foods; and discuss the limitations and future perspectives of Raman spectroscopic methods for food safety surveillance. This review is aimed to emphasize potential opportunities for applying Raman spectroscopic methods as a promising technique for food safety detection.


Assuntos
Contaminação de Alimentos , Inocuidade dos Alimentos/métodos , Análise Espectral Raman/métodos , Suplementos Nutricionais
3.
Se Pu ; 39(2): 162-172, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-34227349

RESUMO

Food safety is closely related to human health and life. Contaminated foods may result in illness or poisoning. For example, perfluorinated compounds can concentrate in the human body, or they can be transferred to the baby during breastfeeding, thus leading to serious health risks. Phthalate esters may cause damage to the liver, lungs, and kidneys. Therefore, food safety has become a hot topic at a global level. Poisonous and harmful substances in foods are derived from the environment, planting or breeding, food contacting materials, and food processing, or due to unsuitable storage conditions. Residues of pesticides and veterinary drugs, organic pollutants, additives, heavy metals, and biotoxins often hamper food safety, causing diseases or even death. The diversity of available food species, complexity of the sample matrix, and lack of information about the source of pollutants render the direct determination of food contaminants difficult. Pretreatment is vital for the accurate analysis of trace toxins in foods. Optimal pretreatment can not only improve the extract efficiency and determination sensitivity, but also prevent instrument contamination. Pretreatment techniques have played an important role in trace determination for complex matrices. Pretreatment methods can be classified as solvent-based and adsorption-based methods. Adsorption-based techniques such as solid-phase extraction, magnetic solid-phase extraction, and solid-phase microextraction are simple and efficient, and hence, are widely used. In these pretreatment techniques, adsorbents play a key role in the extraction effect. In the last few years, metal organic frameworks, metal oxide materials, carbon nanotubes, graphene, and magnetic nanoparticles, as well as a combination of these materials, have been used as adsorbents. These materials are porous and have a large surface area; they are used to enrich trace targets and eliminate interferents. Covalent organic polymers (COPs) are a class of organic porous materials constructed from organic monomers via covalent bonding. Given their excellent characteristics such as light density, good stability, high surface area, structural controllability, and ease of modification, COPs are potential adsorbents. COPs are often synthesized by solvent thermal methods. However, these methods are time-consuming and require toxic solvents and harsh reaction conditions. As alternatives, room-temperature methods, mechanical chemical methods, microwave-assisted methods, and UV-assisted methods have been developed. This has facilitated the synthesis of a wide range of COPs. In this article, the recent applications of COPs in sample pretreatment for food safety analysis are reviewed. COPs can be used in solid-phase extraction by simple packing into columns, polymerization, or chemical bonding in the capillary. Magnetic compounds have been prepared by one-pot synthesis, in situ growth, in situ reduction, or coprecipitation methods and used in magnetic solid-phase extraction. Coatings of solid-phase microextraction fibers are fabricated by physical methods, chemical bonding, sol-gel methods, or in situ growth. Toxic and harmful substances in foods and foodstuffs are efficiently extracted by exploiting the high adsorbent capacities and specificity of COPs. Future development prospects and challenges in sample pretreatment are also discussed herein. There is increased focus on the development of simple, efficient, and environment-friendly methods to synthesize COPs with specific functions; further, high-throughput, sensitive analytical methods may be established. In the future, more specific COPs will be prepared in a cost-effective manner for widespread use in sample pretreatment.


Assuntos
Análise de Alimentos/métodos , Inocuidade dos Alimentos , Estruturas Metalorgânicas , Nanotubos de Carbono , Análise de Perigos e Pontos Críticos de Controle , Polímeros , Extração em Fase Sólida
4.
Biosensors (Basel) ; 11(6)2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34207580

RESUMO

Foodborne disease caused by foodborne pathogens is a very important issue in food safety. Therefore, the rapid screening and sensitive detection of foodborne pathogens is of great significance for ensuring food safety. At present, many research works have reported the application of biosensors and signal amplification technologies to achieve the rapid and sensitive detection of pathogenic bacteria. Thus, this review summarized the use of biosensors coupled with signal amplification technology for the detection of pathogenic bacteria, including (1) the development, concept, and principle of biosensors; (2) types of biosensors, such as electrochemical biosensors, optical biosensors, microfluidic biosensors, and so on; and (3) different kinds of signal amplification technologies applied in biosensors, such as enzyme catalysis, nucleic acid chain reaction, biotin-streptavidin, click chemistry, cascade reaction, nanomaterials, and so on. In addition, the challenges and future trends for pathogenic bacteria based on biosensor and signal amplification technology were also discussed and summarized.


Assuntos
Bactérias , Técnicas Biossensoriais , Microbiologia de Alimentos , Inocuidade dos Alimentos , Nanoestruturas , Ácidos Nucleicos , Tecnologia
5.
Ecotoxicol Environ Saf ; 220: 112404, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34111660

RESUMO

Cadmium (Cd), chromium (Cr), lead (Pb), mercury (Hg) and arsenic (As) are potent toxicants to human health via dietary intake. It is imperative to establish accurate soil thresholds based on soil-plant transfer models and food safety standards for safe agricultural production. This study takes rice genotypes and soil properties into account to derive soil thresholds for five heavy metal(loid)s using the bioconcentration factors (BCF) and species sensitivity distribution (SSD) based on the food safety standard. The BCF generated from two paddy soils was calculated to investigate the sensitivity of heavy metal accumulation in nine rice cultivars in a greenhouse pot experiment. Then, empirical soil-plant transfer models were developed from a middle-sensitivity rice cultivar (Denong 2000, one selected from nine rice) grown in nineteen paddy soils with various soil properties under a proper exogenously metal(loid)s concentration gradient. After normalization, hazardous concentrations from the fifth percentile (HC5) were calculated from the SSD curves, and the derived soil thresholds were obtained from HC5 prediction models that based on the combination of pH and organic carbon (OC) or cation exchange capacity (CEC). The soil Cd threshold derived based on pH and organic carbon (pH < 7.5, OC ≥ 20 g kg-1) was 1.3-fold of those only considering pH, whereas the Pb threshold (pH > 6, CEC ≥ 20 cmolc kg-1) was 3.1 times lower than the current threshold. The derived thresholds for five elements were validated to be reliable through literature data and field experiments. The results suggested that deriving soil heavy metal(loid)s threshold using SSD method and local food safety standards is feasible and also applicable to other crops as well as other regions with potential health risks of toxic elements contamination in agricultural production.


Assuntos
Metais Pesados/normas , Oryza/crescimento & desenvolvimento , Poluentes do Solo/normas , Solo/normas , Arsênio/análise , Arsênio/normas , Cádmio/análise , Cádmio/normas , Cromo/análise , Cromo/normas , Produtos Agrícolas/crescimento & desenvolvimento , Produtos Agrícolas/normas , Inocuidade dos Alimentos , Humanos , Chumbo/análise , Chumbo/normas , Mercúrio/análise , Mercúrio/normas , Metais Pesados/análise , Oryza/química , Oryza/genética , Solo/química , Poluentes do Solo/análise
6.
Artigo em Inglês | MEDLINE | ID: mdl-34071295

RESUMO

Historically, chemicals exceeding maximum allowable exposure levels have been disastrous to underdeveloped countries. The global food industry is primarily affected by toxic chemical substances because of natural and anthropogenic factors. Food safety is therefore threatened due to contamination by chemicals throughout the various stages of food production. Persistent Organic Pollutants (POPs) in the form of pesticides and other chemical substances such as Polychlorinated Biphenyls (PCBs) have a widely documented negative impact due to their long-lasting effect on the environment. This present review focuses on the chemical contamination pathways along the various stages of food production until the food reaches the consumer. The contamination of food can stem from various sources such as the agricultural sector and pollution from industrialized regions through the air, water, and soil. Therefore, it is imperative to control the application of chemicals during food packaging, the application of pesticides, and antibiotics in the food industry to prevent undesired residues on foodstuffs. Ultimately, the protection of consumers from food-related chemical toxicity depends on stringent efforts from regulatory authorities both in developed and underdeveloped nations.


Assuntos
Praguicidas , Bifenilos Policlorados , Monitoramento Ambiental , Poluição Ambiental , Contaminação de Alimentos/análise , Inocuidade dos Alimentos , Praguicidas/análise , Praguicidas/toxicidade , Bifenilos Policlorados/análise , Solo
7.
Molecules ; 26(11)2021 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-34071841

RESUMO

Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.


Assuntos
Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Contaminação de Alimentos/análise , Microbiologia de Alimentos , Inocuidade dos Alimentos , Animais , DNA , Impedância Elétrica , Escherichia coli/metabolismo , Humanos , Listeria monocytogenes/efeitos dos fármacos , Microfluídica , Conformação Molecular , Oxirredução , Peptídeos/química , Potenciometria , Controle de Qualidade
8.
Biosens Bioelectron ; 190: 113386, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34119839

RESUMO

Tailored to the increasing demands for sensing technologies, the fabrication of dual-modal sensing technologies through combining two signal transduction channels into one method has been proposed and drawn considerable attention. The integration of two sensing signals not only promotes the analytical efficiency with reduced assumption, but also improves the analytical performances with enlarged detection linear range, enhanced accuracy, and boosted application flexibility. The two top-rated output signals for developing dual-modal sensors are colorimetric and fluorescent signals because of their outstanding merits for point of care applications and real-time sensitive sensing. Given the rapid development of material chemistry and nanotechnology, the recent decade has witnessed great advance in colorimetric/fluorimetric signal based dual-modal sensing technologies. The new sensing strategy leads to a broad avenue for various applications in disease diagnosis, environmental monitoring and food safety because of the complementary and synergistic effects of the two output signals. In this state-of-the-art review, we comprehensively summarize different types of colorimetric/fluorimetric dual-modal sensing methods by highlighting representative research in the last 5 years, digging into their sensing methodologies, particularly the working principles of the signal transduction systems. Then, the challenges and future prospects for boosting further development of this research field are discussed.


Assuntos
Técnicas Biossensoriais , Colorimetria , Fluorometria , Inocuidade dos Alimentos , Nanotecnologia
9.
J Chromatogr A ; 1651: 462261, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34126375

RESUMO

Contamination of food with chemicals migrating from food contact materials (FCMs) is an important area of food safety. This study was aimed to investigate migration of chemicals from plastic FCMs used for microwave and conventional oven heating. Migration tests were conducted for samples of microwave trays, microwave oven bags, and oven bags. GC- and LC-Orbitrap mass spectrometry (MS) was used for non-targeted screening and identification of chemicals with mass error <5 ppm. A non-targeted identification approach was validated with isotopically labeled chemicals to establish acceptable criteria for identification of migrated compounds. A total of 74 migrated compounds were tentatively identified: 24 chemicals by GC-Orbitrap MS with electron ionization (EI), plus 35 and 19 by LC-Orbitrap MS electrospray ionization (ESI) with positive and negative polarities, respectively. Four migrated chemicals were identified by more than one instrumental analysis. Both intentionally added substances (IAS), i.e. additives used in the production of polymeric materials and plastics, and non-intentionally added substances (NIAS), i.e. derivatives and degradation/oxidation products of IAS, were identified among the migrated chemicals. The levels of 25 migrated chemicals were significantly different (p < 0.05) between microwave treatments and conventional oven treatments, where 20 migrants had higher levels for microwave compared with 5 for conventional oven treatments. For several identified chemicals, no previous reports on their migration from FCMs were found.


Assuntos
Contaminação de Alimentos/análise , Embalagem de Alimentos , Alimentos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Micro-Ondas , Plásticos/análise , Cromatografia Líquida , Inocuidade dos Alimentos , Análise de Componente Principal , Reprodutibilidade dos Testes
10.
Food Res Int ; 145: 110415, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-34112418

RESUMO

Heat treatment is an effective method for ensuring food safety and quality by controlling microbial contamination. However, food poisoning outbreaks have continuously occurred in heat-treated products due to improper thermal treatment and/or post-contamination of foodborne pathogens. This study proposes a novel strategy combining thermostable bacteriophages with thermal processing of food production plants to control foodborne pathogens and even bacterial contamination. Typically, bacteriophages' susceptibility to heat is a major challenge to their application with thermal processing, we isolated thermostable bacteriophages by a modified isolation method of applying heat to samples and characterized the thermostable bacteriophages. Furthermore, we optimized the bacteriophage cocktail components to expand the controllable host range and reduce the risk of bacteriophage resistance development. Finally, we verified this antibacterial strategy by combining heat treatment with thermostable bacteriophages in model systems, including milk and chicken breast. After the phage cocktail and heat treatment, we artificially contaminated the food products to mimic the post-contamination event. Surprisingly, the remaining bacteriophages that withstood heat treatment significantly reduced the number of post-contaminated Salmonella. Altogether, thermostable phages could be applied as complementary tools to control post-contamination after thermal processing of food products.


Assuntos
Bacteriófagos , Doenças Transmitidas por Alimentos , Microbiologia de Alimentos , Inocuidade dos Alimentos , Temperatura Alta , Humanos
11.
Biosensors (Basel) ; 11(5)2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34062907

RESUMO

The development of sensitive methods for the determination of potential bacterial contamination is of upmost importance for environmental monitoring and food safety. In this study, we present a new method combining a fast pre-enrichment step using a microporous cryogel and a detection and identification step using antimicrobial peptides (AMPs) and labelled antibodies, respectively. The experimental method consists of: (i) the capture of large amounts of bacteria from liquid samples by using a highly porous and functionalized cryogel; (ii) the detection and categorisation of Gram-positive and Gram-negative bacteria by determining their affinities toward a small set of AMPs; and (iii) the identification of the bacterial strain by using labelled detection antibodies. As proof of concept, the assessment of the three steps of the analysis was performed by using Escherichia coli and Bacillus sp. as models for Gram-negative and Gram-positive bacteria, respectively. The use of AMPs with broad specificity combined with labelled antibodies enabled the detection and potential categorization of a large spectrum of unknown or unexpected bacteria.


Assuntos
Bactérias/isolamento & purificação , Microbiologia da Água , Bacillus , Monitoramento Ambiental , Escherichia coli , Inocuidade dos Alimentos
12.
Sci Rep ; 11(1): 11898, 2021 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-34099828

RESUMO

Here we document 47,381 individuals from 38 species, including 31 protected species sold between May 2017 and November 2019 in Wuhan's markets. We note that no pangolins (or bats) were traded, supporting reformed opinion that pangolins were not likely the spillover host at the source of the current coronavirus (COVID-19) pandemic. While we caution against the misattribution of COVID-19's origins, the wild animals on sale in Wuhan suffered poor welfare and hygiene conditions and we detail a range of other zoonotic infections they can potentially vector. Nevertheless, in a precautionary response to COVID-19, China's Ministries temporarily banned all wildlife trade on 26th Jan 2020 until the COVID-19 pandemic concludes, and permanently banned eating and trading terrestrial wild (non-livestock) animals for food on 24th Feb 2020. These interventions, intended to protect human health, redress previous trading and enforcement inconsistencies, and will have collateral benefits for global biodiversity conservation and animal welfare.


Assuntos
Animais Selvagens/virologia , COVID-19/etiologia , SARS-CoV-2/patogenicidade , Zoonoses/epidemiologia , Animais , COVID-19/virologia , China , Quirópteros/virologia , Comércio , Inocuidade dos Alimentos , Humanos , Pangolins/microbiologia
13.
Molecules ; 26(10)2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-34063344

RESUMO

Safety and quality are key issues for the food industry. Consequently, there is growing demand to preserve the food chain and products against substances toxic, harmful to human health, such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices, such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long analyses, expensive and complex procedures, and skilled personnel. Therefore, developing performant electrochemical biosensors can significantly support the screening of food chains and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in food screening and their challenges.


Assuntos
Técnicas Biossensoriais/instrumentação , Técnicas Eletroquímicas/instrumentação , Inocuidade dos Alimentos/métodos , Antibacterianos/análise , Cadeia Alimentar , Humanos , Praguicidas/análise , Toxinas Biológicas/análise
15.
Sci Total Environ ; 789: 147948, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34051502

RESUMO

Irrigation water coming from freshwater bodies that suffer toxic cyanobacterial blooms causes adverse effects on crop productivity and quality and raises concerns regarding food contamination and human exposure to toxins. The common agricultural practice of spray irrigation is an important exposure route to cyanotoxins, yet its impact on crops has received little attention. In the present study we attempted an integrated approach at the macro- and microscopic level to investigate whether spray or drip irrigation with microcystins (MCs)-rich water differently affect spinach performance. Growth and functional features, structural characteristics of stomata, and toxin bioaccumulation were determined. Additionally, the impact of irrigation method and water type on the abundance of leaf-attached microorganisms was assessed. Drip irrigation with MCs-rich water had detrimental effects on growth and photosynthetic characteristics of spinach, while spray irrigation ameliorated to various extents the observed impairments. The stomatal characteristics were differently affected by the irrigation method. Drip-irrigated spinach leaves showed significantly lower stomatal density in the abaxial epidermis and smaller stomatal size in the adaxial side compared to spray-irrigation treatment. Nevertheless, the latter deteriorated traits related to fresh produce quality and safety for human consumption; both the abundance of leaf-attached microorganisms and the MCs bioaccumulation in edible tissues well exceeded the corresponding values of drip-irrigated spinach with MC-rich water. The results highlight the significance of both the use of MCs-contaminated water in vegetable production and the irrigation method in shaping plant responses as well as health risk due to human and livestock exposure to MCs.


Assuntos
Microcistinas , Spinacia oleracea , Irrigação Agrícola , Inocuidade dos Alimentos , Humanos , Microcistinas/análise , Microcistinas/toxicidade , Folhas de Planta/química , Água
16.
Food Res Int ; 143: 110246, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33992358

RESUMO

The global burden of foodborne diseases is substantial and foodborne pathogens are the major cause for human illnesses. In order to prevent the spread of foodborne pathogens, detection methods are constantly being updated towards rapid, portable, inexpensive, and multiplexed on-site detection. Due to the nature of the small size and low volume, microfluidics has been applied to rapid, time-saving, sensitive, and portable devices to meet the requirements of on-site detection. Simultaneous detection of multiple pathogens is another key parameter to ensure food safety. Multiplexed detection technology, including microfluidic chip design, offers a new opportunity to achieve this goal. In this review, we introduced several sample preparation and corresponding detection methods on microfluidic devices for multiplexed detection of foodborne pathogens. In the sample preparation section, methods of cell capture and enrichment, as well as nucleic acid sample preparation, were described in detail, and in the section of detection methods, amplification, immunoassay, surface plasmon resonance and impedance spectroscopy were exhaustively illustrated. The limitations and advantages of all available experimental options were also summarized and discussed in order to form a comprehensive understanding of cutting-edge technologies and provide a comparative assessment for future investigation and in-field application.


Assuntos
Doenças Transmitidas por Alimentos , Dispositivos Lab-On-A-Chip , Inocuidade dos Alimentos , Humanos , Microfluídica , Ressonância de Plasmônio de Superfície
17.
Food Chem ; 361: 130039, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34022482

RESUMO

This study aimed to obtain a reliable evaluation about addition of tert-butylhydroquinone (TBHQ), and distribution of TBHQ and 2-tert-butyl-1,4-benzoquinone (TBBQ) contents in typical edible oils and oleaginous foods marketed in Hangzhou City. Briefly, the probability of labeled with addition of TBHQ in foods decreased from 36.45 ± 2.6% to 28.78 ± 3.7% in the period from 2018 to 2020. In the 135 analyzed samples, TBHQ contents were far less than the maximum legal additive amount, and TBBQ contents ranged from below its limit of quantification (LOQ) to 13.54 ± 1.15 mg/kg. The conversion rate from TBHQ to TBBQ in edible oils was 2.94 ± 1.17%, much lower than that in other food categories. Further research determined that the process method and food composition were the main factors for different conversion rates from TBHQ to TBBQ in various food categories. In addition, oil consumption was found to be the primary source of dietary intake of TBHQ and TBBQ.


Assuntos
Benzoquinonas/análise , Hidroquinonas/análise , Óleos Vegetais/análise , China , Análise de Alimentos , Inocuidade dos Alimentos
18.
Food Chem ; 361: 130037, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34029909

RESUMO

In this study, the concentration of furan and 2-methylfuran in espresso coffee (EC) obtained from Arabica and Robusta coffee varieties was determined as a function of specific particle size. The particle size and coffee variety significantly influenced the level of furan and 2-methylfuran. In Arabica variety, furan and 2-methylfuran level increased with increasing particle size. Particularly, from C<200µm to C>425µm fractions, furan increased from 68.27 to 91.48 ng mL-1 while 2-methylfuran from 404.31 to 634.64 ng mL-1. In Robusta variety, the highest concentration of furan and 2-methylfuran occurred in ECs prepared using C300-425µm fraction showing values of 116.39 ng mL-1 and 845.14 ng mL-1, respectively, for furan and 2-methylfuran. On the basis of this experiment, it is possible to establish a mitigation strategy by manipulating the particle size and coffee variety in order to reduce the level of furan and 2-methylfuran in EC up to 11.4% and 18.8%, respectively.


Assuntos
Café/química , Furanos/análise , Inocuidade dos Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Tamanho da Partícula
19.
Food Res Int ; 144: 110341, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34053537

RESUMO

Fermented and/or protein-rich foods, the most widely consumed worldwide, are the most susceptible to the presence of high levels of biogenic amines (BAs). Many reviews have focused on BAs toxicity and presence in foods; however, technological strategies such as evaluation of physical parameters, the addition of natural or synthetic compounds or the use of specific starter cultures of BAs reduction, and quick detection methods have been scarcely approached. In current research, there has been a focus on fast detection of BAs through colorimetric methods that allow these compounds to be quickly and easily identified by consumers. To reduce BAs presence in food, several alternatives have been developed and investigated with the aim of preventing negative effects caused by their intake, which can be applied before, during, or after processing. Food safety is one of the most important concerns of consumer and sanitary authorities. Therefore, detecting toxins such as BAs in food has become a priority for research. Recent reports that focus on the development of rapid detection methods of BAs are reviewed in this analysis. These methods have been successfully applied to food matrices with little to no sample pretreatment. Several alternatives for BAs reduction in food was also summarized. These findings will help the food industry to improve its processes for developing safe food.


Assuntos
Aminas Biogênicas , Manipulação de Alimentos , Aminas Biogênicas/análise , Fermentação , Inocuidade dos Alimentos
20.
Food Res Int ; 144: 110368, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34053561

RESUMO

Without standardized methods for rapidly detecting in food matrices viable T. cruzi, foodborne outbreaks remain neglected. In this work, a reverse-transcriptase real-time PCR (RT-qPCR) mRNA-based technique was developed for the rapid and specific detection and quantification of viable Trypanosoma cruzi in açai fruits and juice. The method uses specific primer targeting region on the cyt b gene. The maximum recovery rate of T. cruzi from inoculated açai juice was 82.50%. The limit of detection and quantification in açai juice was 10 parasites/mL for RT-qPCR (mRNA-based) and qPCR (DNA-based). The RT-qPCR efficiency was estimated at 97.27% with an R2 of 0.994. The RT-qPCR was shown to be able to discriminate between viable and nonviable cells. This method provides a useful tool for rapid assessment of low concentrations of viable T. cruzi in naturally contaminated food samples, and can be applied industrially as a quality and security method.


Assuntos
Doença de Chagas , Trypanosoma cruzi , Doença de Chagas/epidemiologia , Surtos de Doenças , Inocuidade dos Alimentos , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Trypanosoma cruzi/genética
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