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1.
J Am Med Inform Assoc ; 27(11): 1716-1720, 2020 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-33067628

RESUMO

OBJECTIVE: Reducing risk of coronavirus disease 2019 (COVID-19) infection among healthcare personnel requires a robust occupational health response involving multiple disciplines. We describe a flexible informatics solution to enable such coordination, and we make it available as open-source software. MATERIALS AND METHODS: We developed a stand-alone application that integrates data from several sources, including electronic health record data and data captured outside the electronic health record. RESULTS: The application facilitates workflows from different hospital departments, including Occupational Health and Infection Control, and has been used extensively. As of June 2020, 4629 employees and 7768 patients and have been added for tracking by the application, and the application has been accessed over 46 000 times. DISCUSSION: Data captured by the application provides both a historical and real-time view into the operational impact of COVID-19 within the hospital, enabling aggregate and patient-level reporting to support identification of new cases, contact tracing, outbreak investigations, and employee workforce management. CONCLUSIONS: We have developed an open-source application that facilitates communication and workflow across multiple disciplines to manage hospital employees impacted by the COVID-19 pandemic.


Assuntos
Infecções por Coronavirus/transmissão , Gerenciamento de Dados , Pessoal de Saúde , Saúde do Trabalhador , Sistemas de Identificação de Pacientes/métodos , Pneumonia Viral/transmissão , Software , Fluxo de Trabalho , Boston , Surtos de Doenças , Hospitais de Veteranos , Humanos , Transmissão de Doença Infecciosa do Paciente para o Profissional/prevenção & controle , Pandemias , Integração de Sistemas , Estados Unidos
2.
Stud Health Technol Inform ; 273: 3-20, 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-33087589

RESUMO

Multidisciplinary and highly dynamic pHealth ecosystems according to the 5P Medicine paradigm require careful consideration of systems integration and interoperability within the domains knowledge space. The paper addresses the different aspects or levels of knowledge representation (KR) and management (KM) from cognitive theories (theories of knowledge) and modeling processes through notation up to processing, tooling and implementation. Thereby, it discusses language and grammar challenges and constraints, but also development process aspects and solutions, so demonstrating the limitation of data level considerations. Finally, it presents the ISO 23903 Interoperability and Integration Reference Architecture to solve the addressed problems and to correctly deploy existing standards and work products at any representational level including data models as well as data model integration and interoperability.


Assuntos
Ecossistema , Integração de Sistemas , Idioma
3.
Sportis (A Coruña) ; 6(3): 408-425, sept. 2020. tab
Artigo em Espanhol | IBECS | ID: ibc-197056

RESUMO

Los métodos educativos de integración del movimiento son reconocidos como potenciadores de la actividad física, de las funciones ejecutivas y del aprendizaje. Sin embargo, el impacto de estas metodologías sobre la cognición y la actividad física en Educación Infantil ha sido poco estudiado. El objetivo de la investigación fue analizar el efecto de un programa educativo de integración del movimiento basado en el juego en esta etapa, evaluando sus efectos sobre los niveles de actividad física de los niños. Participaron 134 alumnos de 5 años de la provincia de Málaga. Se diseñó e implementó un programa de integración del movimiento basado en actividades de juego motor semi-dirigido que cambiaban cada quince minutos, en las cuales se trabajaron contenidos académicos. Se evaluó la cantidad e intensidad de actividad física mediante acelerometría (Actigraph GT3X). Durante su participación en el programa, los niños realizaron una media de 45,65 minutos de actividad física moderada-vigorosa. El porcentaje más alto corresponde a la actividad sedentaria y a la actividad moderada-vigorosa respectivamente, lo cual concuerda con estudios que afirman que la actividad de los niños pequeños consiste en ráfagas cortas de actividad moderada-vigorosa intercaladas con períodos de menor intensidad. Los datos muestran que el juego como método de integración del movimiento contribuye a alcanzar los niveles de actividad recomendados por las organizaciones internacionales. Nuestros resultados apoyan el uso del juego semi-dirigido como una herramienta especialmente útil para la mejora del proceso de enseñanza-aprendizaje en Educación Infantil y para el aumento de la actividad física del alumnado


Educational movement integration methods have been recognized as enhancers of physical activity, executive functions and learning. However, the impact of these methodologies on cognition and physical activity in Early Childhood Education has been little studied. The aim of this study was to analyze the effect of an educational play-based movement integration program at this stage, evaluating its effects on children's physical activity levels. A total of 134 5-year-old students from the province of Malaga participated. We designed and implemented a movement integration program based on semi-directed motor play activities that changed every fifteen minutes, in which academic content was worked on. The amount and intensity of physical activity was evaluated using accelerometry (Actigraph GT3X). During their participation in the program, children carried out an average of 45.65 minutes of moderate-vigorous physical activity. The highest percentage corresponds to sedentary activity and moderate-vigorous activity respectively, which is consistent with other studies that state the activity of young children consists of short bursts of moderate-vigorous activity interspersed with less-intensity periods. Data shows that play as a movement integration method contributes to reaching the activity levels recommended by international organizations. Our results support the use of semi-directed play as an especially useful tool for the improvement of the teaching-learning process in Early Childhood Education and for increasing students' physical activity


Assuntos
Humanos , Masculino , Feminino , Criança , Adulto , Jogos e Brinquedos , Movimento/fisiologia , Atividade Motora/fisiologia , Educação em Saúde/métodos , Inclusão Escolar/métodos , Integração de Sistemas , Promoção da Saúde , Acelerometria/métodos
5.
Med Teach ; 42(10): 1123-1127, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32776858

RESUMO

The current global crises, including climate, COVID-19, and environmental change, requires global collective action at all scales. These broad socio-ecological challenges require the engagement of diverse perspectives and ways of knowing and the meaningful engagement of all generations and stages of personal and professional development. The combination of systems thinking, change management, quality improvement approaches and models, appreciative/strength-based approaches, narratives, storytelling and the strengths of Indigenous knowledges, offer synergies and potential that can set the stage for transformative, strengths-based education for sustainable healthcare (ESH). The need for strong leadership to enact a vision for ESH is outlined here with the intent to enable and nurture the conditions for change, ultimately improving health and well-being across generations.


Assuntos
Infecções por Coronavirus/epidemiologia , Assistência à Saúde/organização & administração , Educação Médica/organização & administração , Pneumonia Viral/epidemiologia , Betacoronavirus , Humanos , Relações Interprofissionais , Modelos Organizacionais , Pandemias , Integração de Sistemas
6.
Am J Infect Control ; 48(10): 1244-1247, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32763351

RESUMO

The coronavirus disease 2019 (COVID-19) pandemic has created many challenges for health care providers. At the forefront is the need to balance optimal patient care with the safety of those providing that care. This is especially true during resuscitations where life-saving procedures cause widespread aerosolization of the virus. Efforts to mitigate this exposure to front-line providers are therefore paramount. We share how we used simulation to prepare our pediatric emergency department for COVID-19 resuscitations.


Assuntos
Infecções por Coronavirus/terapia , Serviço Hospitalar de Emergência , Pessoal de Saúde/educação , Pneumonia Viral/terapia , Ressuscitação/educação , Treinamento por Simulação/métodos , Betacoronavirus , Criança , Humanos , Pandemias , Integração de Sistemas
7.
PLoS One ; 15(7): e0235690, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32634158

RESUMO

The identification of potential super-spreader nodes within a network is a critical part of the study and analysis of real-world networks. Motivated by a new interpretation of the "shortest path" between two nodes, this paper explores the properties of the heatmap centrality by comparing the farness of a node with the average sum of farness of its adjacent nodes in order to identify influential nodes within the network. As many real-world networks are often claimed to be scale-free, numerical experiments based upon both simulated and real-world undirected and unweighted scale-free networks are used to illustrate the effectiveness of the proposed "shortest path" based measure with regards to its CPU run time and ranking of influential nodes.


Assuntos
Algoritmos , Teoria de Sistemas , Simulação por Computador , Sistemas Computacionais , Métodos Epidemiológicos , Integração de Sistemas
8.
Stud Health Technol Inform ; 271: 153-160, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32578558

RESUMO

The use of standards and Integrating the Healthcare Enterprise (IHE) is a crucial technique for supporting, monitoring and controlling the integration profiles in healthcare organization systems. The risk of "no-interoperability" increases when several standards are used. Lack of experience in developing countries, particularly in Algeria, increases this risk. This article aims to suggest and present a methodology for selecting standards and IHE integration profiles in field of E-health in order to build a base-frame of the Algerian E-Health system. In fact, this base-frame must comply with international standards and perfectly adapted to medical processes, thru respecting IHE integration profiles.


Assuntos
Telemedicina , Argélia , Assistência à Saúde , Programas Governamentais , Integração de Sistemas
9.
Stud Health Technol Inform ; 270: 13-17, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32570337

RESUMO

Healthcare 4.0 demands healthcare data to be shaped into a common standardized and interoperable format for achieving more efficient data exchange. Most of the techniques addressing this domain are dealing only with specific cases of data transformation through the translation of healthcare data into ontologies, which usually result in clinical misinterpretations. Currently, ontology alignment techniques are used to match different ontologies based on specific string and semantic similarity metrics, where very little systematic analysis has been performed on which semantic similarity techniques behave better. For that reason, in this paper we are investigating on finding the most efficient semantic similarity technique, based on an existing approach that can transform any healthcare dataset into HL7 FHIR, through the translation of the latter into ontologies, and their matching based on syntactic and semantic similarities.


Assuntos
Ontologias Biológicas , Recursos em Saúde , Semântica , Assistência à Saúde , Registros Eletrônicos de Saúde , Integração de Sistemas
10.
Stud Health Technol Inform ; 270: 267-271, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32570388

RESUMO

Information relevant to pharmacogenomics studies is available in several open databases, which makes it difficult to synthetize the available data. Within the PractikPharma project, several databases were integrated to PGxLOD, a resource dedicated to the generation and verification of pharmacogenomic influence on drug responses. The Comparative Toxicogenomic Database (CTD) describes the toxic effects of many chemicals on living species based on the literature. Since drugs are peculiar chemicals and side effects are peculiar toxic effects, we aimed at extracting information from CTD that matches drug side effects in the human specie.


Assuntos
Doença/etiologia , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Substâncias Perigosas/toxicidade , Farmacogenética , Toxicogenética , Bases de Dados Factuais , Doença/genética , Humanos , Pesquisa , Integração de Sistemas
11.
Stud Health Technol Inform ; 270: 402-406, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32570415

RESUMO

The integration of health data systems is an open problem. Most of the active initiatives are based on the use of standards, each one proposed for a concrete use, without integrating other needs or standards allowing on homogenous use. We propose an alternative to get an unified view of health related data, valid for several uses, that can integrate heterogeneous sources. The proposal set the framework to integrate the developments made so far to automatically learn the extraction and conversion of the data. All the sources are integrated under a single access point. We present the main concepts of EHRagg as a middleware between systems that can incorporate several sources giving an unified access following the FAIR principles.


Assuntos
Registros Eletrônicos de Saúde , Integração de Sistemas
12.
PLoS One ; 15(5): e0231872, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32374724

RESUMO

BACKGROUND: Depression is highly prevalent among patients newly starting antiretroviral treatment (ART) in Malawi and many other countries. Untreated depression at ART initiation can disrupt the HIV care continuum. Effective approaches for depression screening and treatment exist for low-resource settings, but they are rarely applied. Identifying effective implementation strategies are critical. METHODS: A pilot program integrated depression screening and treatment into routine HIV care using existing staff at two public health clinics in Malawi in two phases; a screening-only "control" phase and an active "intervention" phase. During the intervention phase, providers prescribed antidepressants or referred patients for Friendship Bench problem-solving therapy. We evaluated the program's impact on retention in HIV care, viral suppression, and depression remission at 6 months using tabular comparisons and log-binomial models to estimate adjusted risk ratios and mean differences among the intervention group relative to the control group. RESULTS: Nearly all consenting participants were screened for depression appropriately and 25% had mild to severe depressive symptoms. During the intervention phase, 86% of participants with mild depressive symptoms started Friendship Bench therapy and 96% of participants with moderate to severe depressive symptoms started antidepressants. Few participants in the intervention group received consistent depression treatment over their first 6 months in care. In the adjusted main analysis, program exposure did not demonstrably affect most HIV or mental health outcomes, though the probability of currently being on ART at 6 months was significantly lower among the intervention group than the control group [RR 0.6(95%CI: 0.4-0.9)]. CONCLUSIONS: While it is feasible to integrate depression screening and treatment initiation into ART initiation, providing ongoing depression treatment over time is challenging. Similar implementation science studies focused on maintaining depression management will be increasingly important as we strive to understand and test the best ways to implement evidence-based depression treatment within HIV care.


Assuntos
Antirretrovirais/uso terapêutico , Assistência à Saúde , Depressão/diagnóstico , Infecções por HIV/tratamento farmacológico , Infecções por HIV/psicologia , Programas de Rastreamento/organização & administração , Adulto , Antidepressivos/uso terapêutico , Assistência à Saúde/métodos , Assistência à Saúde/organização & administração , Depressão/complicações , Depressão/epidemiologia , Depressão/terapia , Estudos de Viabilidade , Feminino , HIV , Infecções por HIV/complicações , Infecções por HIV/epidemiologia , Humanos , Ciência da Implementação , Malaui/epidemiologia , Masculino , Programas de Rastreamento/métodos , Saúde Mental , Participação do Paciente/estatística & dados numéricos , Projetos Piloto , Avaliação de Programas e Projetos de Saúde , Psicoterapia/métodos , Psicoterapia/organização & administração , Indução de Remissão , Integração de Sistemas , Resultado do Tratamento , Carga Viral/efeitos dos fármacos , Adulto Jovem
14.
Nat Med ; 26(4): 511-518, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32251406

RESUMO

Cellular immunity is critical for controlling intracellular pathogens, but individual cellular dynamics and cell-cell cooperativity in evolving human immune responses remain poorly understood. Single-cell RNA-sequencing (scRNA-seq) represents a powerful tool for dissecting complex multicellular behaviors in health and disease1,2 and nominating testable therapeutic targets3. Its application to longitudinal samples could afford an opportunity to uncover cellular factors associated with the evolution of disease progression without potentially confounding inter-individual variability4. Here, we present an experimental and computational methodology that uses scRNA-seq to characterize dynamic cellular programs and their molecular drivers, and apply it to HIV infection. By performing scRNA-seq on peripheral blood mononuclear cells from four untreated individuals before and longitudinally during acute infection5, we were powered within each to discover gene response modules that vary by time and cell subset. Beyond previously unappreciated individual- and cell-type-specific interferon-stimulated gene upregulation, we describe temporally aligned gene expression responses obscured in bulk analyses, including those involved in proinflammatory T cell differentiation, prolonged monocyte major histocompatibility complex II upregulation and persistent natural killer (NK) cell cytolytic killing. We further identify response features arising in the first weeks of infection, for example proliferating natural killer cells, which potentially may associate with future viral control. Overall, our approach provides a unified framework for characterizing multiple dynamic cellular responses and their coordination.


Assuntos
Comunicação Celular , Infecções por HIV/genética , Infecções por HIV/imunologia , Imunidade Celular/fisiologia , Análise de Célula Única/métodos , Doença Aguda , Reação de Fase Aguda/genética , Reação de Fase Aguda/imunologia , Reação de Fase Aguda/patologia , Adolescente , Adulto , Comunicação Celular/genética , Comunicação Celular/imunologia , Feminino , Perfilação da Expressão Gênica , Redes Reguladoras de Genes/imunologia , Infecções por HIV/patologia , HIV-1/genética , HIV-1/patogenicidade , Humanos , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Leucócitos Mononucleares/metabolismo , Leucócitos Mononucleares/patologia , Estudos Longitudinais , Análise de Sequência de RNA/métodos , Integração de Sistemas , Linfócitos T Citotóxicos/imunologia , Linfócitos T Citotóxicos/metabolismo , Carga Viral/genética , Carga Viral/imunologia , Adulto Jovem
15.
Neuron ; 106(3): 388-403.e18, 2020 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-32142648

RESUMO

Structural rules underlying functional properties of cortical circuits are poorly understood. To explore these rules systematically, we integrated information from extensive literature curation and large-scale experimental surveys into a data-driven, biologically realistic simulation of the awake mouse primary visual cortex. The model was constructed at two levels of granularity, using either biophysically detailed or point neurons. Both variants have identical network connectivity and were compared to each other and to experimental recordings of visual-driven neural activity. While tuning these networks to recapitulate experimental data, we identified rules governing cell-class-specific connectivity and synaptic strengths. These structural constraints constitute hypotheses that can be tested experimentally. Despite their distinct single-cell abstraction, both spatially extended and point models perform similarly at the level of firing rate distributions for the questions we investigated. All data and models are freely available as a resource for the community.


Assuntos
Modelos Neurológicos , Neurônios/fisiologia , Córtex Visual/fisiologia , Animais , Camundongos , Sinapses/fisiologia , Integração de Sistemas , Córtex Visual/citologia
16.
BMC Med Inform Decis Mak ; 20(1): 53, 2020 03 11.
Artigo em Inglês | MEDLINE | ID: mdl-32160884

RESUMO

BACKGROUND: Informatics tools to support the integration and subsequent interrogation of spatiotemporal data such as clinical data and environmental exposures data are lacking. Such tools are needed to support research in environmental health and any biomedical field that is challenged by the need for integrated spatiotemporal data to examine individual-level determinants of health and disease. RESULTS: We have developed an open-source software application-FHIR PIT (Health Level 7 Fast Healthcare Interoperability Resources Patient data Integration Tool)-to enable studies on the impact of individual-level environmental exposures on health and disease. FHIR PIT was motivated by the need to integrate patient data derived from our institution's clinical warehouse with a variety of public data sources on environmental exposures and then openly expose the data via ICEES (Integrated Clinical and Environmental Exposures Service). FHIR PIT consists of transformation steps or building blocks that can be chained together to form a transformation and integration workflow. Several transformation steps are generic and thus can be reused. As such, new types of data can be incorporated into the modular FHIR PIT pipeline by simply reusing generic steps or adding new ones. We validated FHIR PIT in the context of a driving use case designed to investigate the impact of airborne pollutant exposures on asthma. Specifically, we replicated published findings demonstrating racial disparities in the impact of airborne pollutants on asthma exacerbations. CONCLUSIONS: While FHIR PIT was developed to support our driving use case on asthma, the software can be used to integrate any type and number of spatiotemporal data sources at a level of granularity that enables individual-level study. We expect FHIR PIT to facilitate research in environmental health and numerous other biomedical disciplines.


Assuntos
Registros Eletrônicos de Saúde , Exposição Ambiental , Interoperabilidade da Informação em Saúde/normas , Design de Software , Software , Nível Sete de Saúde , Humanos , Análise Espaço-Temporal , Integração de Sistemas , Fluxo de Trabalho
17.
PLoS One ; 15(2): e0228400, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32027667

RESUMO

Obesity is a multifactorial disease caused by complex interactions between genes and dietary factors. Salt-rich diet is related to the development and progression of several chronic diseases including obesity. However, the molecular basis of how salt sensitivity genes (SSG) contribute to adiposity in obesity patients remains unexplored. In this study, we used the microarray expression data of visceral adipose tissue samples and constructed a complex protein-interaction network of salt sensitivity genes and their co-expressed genes to trace the molecular pathways connected to obesity. The Salt Sensitivity Protein Interaction Network (SSPIN) of 2691 differentially expressed genes and their 15474 interactions has shown that adipose tissues are enriched with the expression of 23 SSGs, 16 hubs and 84 bottlenecks (p = 2.52 x 10-16) involved in diverse molecular pathways connected to adiposity. Fifteen of these 23 SSGs along with 8 other SSGs showed a co-expression with enriched obesity-related genes (r ≥ 0.8). These SSGs and their co-expression partners are involved in diverse metabolic pathways including adipogenesis, adipocytokine signaling pathway, renin-angiotensin system, etc. This study concludes that SSGs could act as molecular signatures for tracing the basis of adipogenesis among obese patients. Integrated network centered methods may accelerate the identification of new molecular targets from the complex obesity genomics data.


Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Redes Reguladoras de Genes/efeitos dos fármacos , Obesidade Pediátrica/genética , Estresse Salino/genética , Cloreto de Sódio na Dieta/farmacologia , Adiposidade/efeitos dos fármacos , Adiposidade/genética , Adolescente , Estudos de Casos e Controles , Epistasia Genética/efeitos dos fármacos , Feminino , Perfilação da Expressão Gênica/métodos , Humanos , Análise em Microsséries/métodos , Obesidade Pediátrica/metabolismo , Obesidade Pediátrica/patologia , Estresse Salino/efeitos dos fármacos , Integração de Sistemas , Adulto Jovem
18.
Sci Rep ; 10(1): 1224, 2020 01 27.
Artigo em Inglês | MEDLINE | ID: mdl-31988326

RESUMO

The genetic etiology of childhood cancers still remains largely unknown. It is therefore essential to develop novel strategies to unravel the spectrum of pediatric cancer genes. Statistical network modeling techniques have emerged as powerful methodologies for enabling the inference of gene-disease relationship and have been performed on adult but not pediatric cancers. We performed a deep multi-layer understanding of pan-cancer transcriptome data selected from the Treehouse Childhood Cancer Initiative through a co-expression network analysis. We identified six modules strongly associated with pediatric tumor histotypes that were functionally linked to developmental processes. Topological analyses highlighted that pediatric cancer predisposition genes and potential therapeutic targets were central regulators of cancer-histotype specific modules. A module was related to multiple pediatric malignancies with functions involved in DNA repair and cell cycle regulation. This canonical oncogenic module gathered most of the childhood cancer predisposition genes and clinically actionable genes. In pediatric acute leukemias, the driver genes were co-expressed in a module related to epigenetic and post-transcriptional processes, suggesting a critical role of these pathways in the progression of hematologic malignancies. This integrative pan-cancer study provides a thorough characterization of pediatric tumor-associated modules and paves the way for investigating novel candidate genes involved in childhood tumorigenesis.


Assuntos
Perfilação da Expressão Gênica/métodos , Redes Reguladoras de Genes/genética , Neoplasias/genética , Biomarcadores Tumorais/genética , Criança , Pré-Escolar , Biologia Computacional/métodos , Simulação por Computador , Bases de Dados Genéticas , Feminino , Expressão Gênica/genética , Regulação da Expressão Gênica/genética , Regulação Neoplásica da Expressão Gênica/genética , Predisposição Genética para Doença/genética , Genômica/métodos , Humanos , Masculino , Modelos Estatísticos , Neoplasias/etiologia , Mapeamento de Interação de Proteínas/métodos , Mapas de Interação de Proteínas/genética , Mapas de Interação de Proteínas/fisiologia , Integração de Sistemas , Transcriptoma/genética
19.
J Am Acad Child Adolesc Psychiatry ; 59(3): 348-349, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31926223

RESUMO

Unraveling the altered brain-behavior relationships in autism spectrum disorder (ASD) has been challenging because of the limitations of sample size and methodologies that are still evolving. Recently, pediatric neuroimaging has undergone considerable advancement by harnessing resting-state functional magnetic resonance imaging (rfMRI),1 in which methodologies can be applied to quantify functional connectivity (FC) from spontaneous fluctuations of brain activity. Benefiting from relatively easy data collection from clinical samples and the ability to harmonize these samples, rfMRI has supported the emergence of open pediatric neuroimaging science (OPENS) through the pooling and sharing of large-scale neuroimaging data by and for the research community (eg, the Autism Brain Imaging Data Exchange [ABIDE]2,3). Big data OPENS ASD studies have revealed functional impairments in both sensory and cognitive brain networks. However, whether these impairments reflect a miswired connectome of network interplay in ASD remains to be elucidated.


Assuntos
Transtorno do Espectro Autista , Conectoma , Transtorno do Espectro Autista/diagnóstico por imagem , Encéfalo/diagnóstico por imagem , Mapeamento Encefálico , Criança , Cognição , Humanos , Imagem por Ressonância Magnética , Vias Neurais/diagnóstico por imagem , Integração de Sistemas
20.
Anal Bioanal Chem ; 412(3): 611-620, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31900539

RESUMO

Infections caused by foodborne microorganisms are a great threat to the global environment and public healthcare today. Thus, rapid, portable and sensitive assays that can realize the identification of foodborne bacteria are highly desired. In this study, a smart fluorescent and colorimetric dual-readout sensing system has been established for simple and rapid E. coli determination by utilizing the Cu2+-triggered oxidation of o-phenylenediamine (OPD). Initially, Cu2+ can oxidize OPD to OPDox, resulting in an orange-yellow fluorescence and visible pale-yellow color. However, E. coli can effectively reduce Cu2+ into Cu+, inhibiting the Cu2+-triggered oxidation of OPD to OPDox. Consequently, the introduction of E. coli can turn off both the fluorescence and the UV-vis absorbance signals of the OPD-Cu2+ system, illustrating an original mechanism for fluorescent and colorimetric dual-channel detection of E. coli. Moreover, a filter paper-based visual sensor was built and coupled with OPD-Cu2+ solution under the assistance of a UV lamp. The as-prepared sensor can detect E. coli quantitatively with the help of a typical smartphone color-scanning application (APP). Thus, this study offers a valid dual-mode assay for sensitive and on-site visible detection of E. coli, guaranteeing the reliability of the results and is more attractive for practical use. Graphical Abstract Schematic illustration of the smartphone-integrated sensing system for fluorescent and colorimetric dual-channel detection of E. coli based on the Cu2+-OPD system.


Assuntos
Bactérias/isolamento & purificação , Colorimetria/métodos , Corantes Fluorescentes/química , Microbiologia de Alimentos , Papel , Smartphone , Espectrometria de Fluorescência/métodos , Integração de Sistemas , Bactérias/patogenicidade , Técnicas Biossensoriais , Reprodutibilidade dos Testes , Espectrofotometria Ultravioleta/métodos
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